ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.87 0.82 0.82 0.7 0.71 0.54 0.5 0.61 0.51 0.43 0.38 0.38 0.97 0.97 0.95 0.49 0.47 0.52 0.69 0.61 0.45 0.78 0.73 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.07 0.07 0.21 0.27 0.52 0.59 0.34 0.52 0.67 0.72 0.73 0 0 0 0.05 0 0 0.05 0 0.24 0 0 0
Nucleus 0 0 0 0 0 0.05 0.06 0.05 0 0 0.06 0 0 0 0 0.15 0.15 0.12 0.06 0.11 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.18 0.19 0.21 0.11 0.13 0.09 0.12 0.13 0.1 0.09 0.08 0 0.07 0.09 0.39 0.38 0.43 0.12 0.15 0.12 0.08 0.09 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 12 1 6 8
Bud 1 0 2 1 7 12 10 4 20 16 16 17 0 0 0 0 0 1 1 2 11 2 9 18
Bud Neck 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 3 5 2 6 5
Bud Site 0 0 0 0 1 3 1 0 2 4 12 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 0 0 1 1 1 2 1 3 3 0 0 0 0 0 0 0 1 2 0 2 0
Cytoplasm 454 204 504 202 290 414 397 491 436 389 282 258 394 348 499 77 83 79 385 183 306 262 386 431
Endoplasmic Reticulum 3 1 0 0 1 2 1 1 2 0 0 0 1 0 2 10 13 6 2 1 9 0 1 3
Endosome 0 0 15 7 8 3 5 26 9 3 2 2 4 4 9 11 9 11 7 1 14 1 4 7
Golgi 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 1 2 1 1 2 7 0 1 2
Mitochondria 66 17 45 61 112 400 472 271 449 608 538 492 0 0 0 8 3 6 26 9 163 5 20 24
Nucleus 4 7 4 9 10 37 45 38 33 30 44 26 1 0 1 23 27 18 32 31 25 16 18 8
Nuclear Periphery 2 1 0 0 0 3 0 0 2 1 6 6 0 0 1 0 0 0 1 0 2 0 2 2
Nucleolus 0 1 0 0 0 1 1 1 2 2 1 1 0 1 0 1 0 0 0 1 1 0 0 0
Peroxisomes 0 0 1 0 0 1 0 0 0 0 0 1 0 1 0 0 1 1 0 0 1 0 0 0
SpindlePole 0 0 2 2 1 4 2 21 12 10 11 11 1 0 0 1 1 0 13 10 18 6 14 17
Vac/Vac Membrane 46 45 118 62 47 96 74 97 109 91 68 55 18 26 49 62 67 66 65 44 82 28 47 43
Unique Cell Count 523 249 613 289 409 767 795 806 857 905 746 676 405 358 523 157 175 152 563 299 686 337 528 590
Labelled Cell Count 576 277 694 344 478 978 1009 951 1078 1155 984 875 420 380 561 194 206 189 563 299 686 337 528 590


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.3 4.5 4.4 4.4 3.5 3.4 3.5 3.3 3.1 2.9 2.8 5.8 5.8 5.5 6.3 5.9 6.2 5.7 5.9 6.1
Std Deviation (1e-4) 0.5 0.9 4.8 2.1 2.1 1.6 1.5 1.1 1.0 0.8 1.0 0.6 1.2 1.5 1.2 1.2 1.2 1.6 1.6 1.3 1.6
Intensity Change (Log2) – – – -0.04 -0.05 -0.36 -0.4 -0.38 -0.44 -0.55 -0.65 -0.69 0.36 0.36 0.28 0.48 0.37 0.46 0.34 0.37 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.3 5.2 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1721 1.0874 0.6932 0.4308 0.446 0.6499 0.013 1.0546 0.7315 0.3056 0.4628 0.8046 -0.5384 0.5372 0.1056 0.0997 -0.3676 0.2387
Actin 0.0047 0.001 0.0049 0.0003 0.0002 0.0003 0.0008 0.0004 0.003 0.0003 0.0009 0.0008 0.0003 0.0003 0.0006 0.0005 0.0005 0.0013
Bud 0.0001 0.0043 0.0024 0.0001 0.0001 0.0011 0.0002 0.0003 0.0005 0.0003 0.0002 0.0003 0.0006 0.004 0.0019 0.0001 0.0001 0.0003
Bud Neck 0.0002 0.0004 0.0004 0.0004 0.0012 0.0024 0.0052 0.001 0.0007 0.0013 0.002 0.01 0.0048 0.0008 0.0004 0.0004 0.0003 0.0067
Bud Periphery 0.0001 0.0019 0.0006 0.0001 0 0.0003 0.0001 0.0001 0.0006 0.0003 0.0003 0.0003 0.0004 0.0009 0.0012 0 0.0001 0.0003
Bud Site 0.0002 0.0083 0.004 0.0002 0.0002 0.0005 0.0011 0.0031 0.0019 0.0005 0.0002 0.0005 0.0098 0.0084 0.0015 0.0003 0.0004 0.0003
Cell Periphery 0.0001 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0.0002 0.0001 0 0.0001 0
Cytoplasm 0.2816 0.3442 0.3101 0.3292 0.3779 0.3338 0.2963 0.3452 0.2398 0.3683 0.248 0.3198 0.3441 0.3776 0.3404 0.4564 0.2063 0.4299
Cytoplasmic Foci 0.0116 0.0176 0.0123 0.0094 0.0109 0.0255 0.0111 0.0081 0.0092 0.0129 0.0119 0.0123 0.0107 0.0066 0.0118 0.0113 0.0184 0.0114
Eisosomes 0.0002 0.0002 0.0002 0.0001 0 0.0001 0 0.0001 0.0001 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0041 0.0052 0.005 0.0053 0.0065 0.0035 0.0041 0.0035 0.0035 0.0027 0.0029 0.0038 0.0059 0.0051 0.0036 0.0055 0.0041 0.0045
Endosome 0.0057 0.0095 0.0101 0.0078 0.0108 0.0071 0.0054 0.0065 0.0042 0.0081 0.0078 0.0073 0.0036 0.0068 0.0064 0.0082 0.0078 0.0071
Golgi 0.0006 0.0004 0.0011 0.0002 0.0001 0.0002 0.0003 0.0003 0.0003 0.0002 0.0003 0.0002 0.0001 0.0002 0.0003 0.0001 0.0003 0.0004
Lipid Particles 0.0036 0.0045 0.0012 0.0003 0.0003 0.0007 0.0014 0.0004 0.0005 0.0005 0.0003 0.0004 0.0002 0.0003 0.0013 0.0002 0.0003 0.0003
Mitochondria 0.0004 0.0011 0.0017 0.001 0.0007 0.0008 0.0008 0.0008 0.0013 0.001 0.0018 0.0008 0.0005 0.0007 0.0005 0.0011 0.0004 0.0016
None 0.5649 0.4344 0.5223 0.5556 0.318 0.4546 0.4611 0.3932 0.4594 0.2747 0.3434 0.3366 0.4849 0.4074 0.4084 0.2524 0.5484 0.2613
Nuclear Periphery 0.0049 0.0145 0.0098 0.0069 0.0144 0.0055 0.0096 0.008 0.0121 0.0108 0.0104 0.0096 0.0128 0.0093 0.0065 0.0392 0.0037 0.0087
Nucleolus 0.0006 0.0024 0.0017 0.001 0.0008 0.0015 0.0018 0.0038 0.0025 0.0046 0.0085 0.0039 0.001 0.0014 0.0009 0.0009 0.0005 0.0023
Nucleus 0.0992 0.129 0.0943 0.0732 0.2448 0.1413 0.166 0.1775 0.2077 0.2636 0.2673 0.2342 0.1037 0.1479 0.202 0.166 0.2005 0.2297
Peroxisomes 0.0096 0.0006 0.0011 0.0001 0.0001 0.0003 0.0009 0.0003 0.0017 0.0006 0.0007 0.0003 0.0001 0.0001 0.0004 0.0002 0.0003 0.0009
Punctate Nuclear 0.0047 0.0156 0.0129 0.0048 0.0091 0.018 0.0309 0.0441 0.0491 0.0451 0.0912 0.0562 0.0127 0.0177 0.0095 0.0535 0.0061 0.0304
Vacuole 0.0025 0.004 0.0029 0.0034 0.0031 0.0021 0.0022 0.003 0.0016 0.0035 0.0014 0.002 0.0029 0.0037 0.0018 0.0026 0.0013 0.0021
Vacuole Periphery 0.0003 0.0007 0.0007 0.0005 0.0006 0.0003 0.0004 0.0004 0.0004 0.0005 0.0004 0.0004 0.0006 0.0005 0.0003 0.0009 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.9963 13.0072 18.7675 15.7956 17.2931 24.2372 14.9781 9.8627 11.0687 14.6119
Translational Efficiency 0.979 1.4634 0.7755 0.8061 0.7188 0.5755 0.9244 1.0196 0.7704 0.8081

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1927 799 2076 1124 1517 2604 48 2782 3444 3403 2124 3906

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 684.72 728.92 816.84 838.74 652.36 727.68 968.06 926.82 670.47 727.97 820.26 901.47
Standard Deviation 74.84 104.58 94.07 134.13 72.61 86.46 129.19 139.23 75.59 91.04 97.63 143.43
Intensity Change Log 2 — 0.090246 0.254539 0.292710 — 0.157636 0.569428 0.506621 — 0.123519 0.416757 0.401040

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000080 0.000989 0.000201 0.000881 0.000061 0.000529 0.000756 0.001093 0.000071 0.000637 0.000213 0.001032
Bud Neck 0.008182 0.022819 0.010398 0.015575 0.004419 0.025272 0.002389 0.023716 0.006525 0.024696 0.010217 0.021373
Bud Site 0.001626 0.014979 0.006116 0.025572 0.001243 0.012207 0.001601 0.025215 0.001458 0.012858 0.006014 0.025318
Cell Periphery 0.000108 0.000389 0.000117 0.000227 0.000133 0.000143 0.000149 0.000171 0.000119 0.000200 0.000118 0.000188
Cytoplasm 0.406216* 0.332689* 0.119504* 0.252586* 0.389571* 0.396439* 0.039847 0.345466* 0.398885* 0.381471* 0.117704* 0.318738*
Cytoplasmic Foci 0.111401 0.173675 0.001269 0.005581 0.116075 0.086650 0.003355 0.003715 0.113460 0.107083 0.001316 0.004252
Eisosomes 0.000073 0.000116 0.000015 0.000038 0.000081 0.000057 0.000088 0.000019 0.000077 0.000070 0.000017 0.000025
Endoplasmic Reticulum 0.000671 0.002129 0.002486 0.008666 0.000679 0.000945 0.012167 0.001414 0.000674 0.001223 0.002705 0.003501
Endosome 0.003209 0.014163 0.000585 0.004207 0.002980 0.007213 0.003009 0.003172 0.003108 0.008845 0.000639 0.003470
Golgi 0.000622 0.003214 0.000037 0.003470 0.000522 0.002596 0.000117 0.002916 0.000578 0.002741 0.000039 0.003075
Lipid Particles 0.006126 0.007333 0.000358 0.001132 0.007344 0.004212 0.003074 0.000452 0.006663 0.004945 0.000419 0.000648
Mitochondria 0.005344 0.013404 0.001172 0.006819 0.002453 0.012103 0.000477 0.006511 0.004071 0.012409 0.001156 0.006600
Mitotic Spindle 0.002094 0.012202 0.003893 0.034833 0.001918 0.017042 0.001081 0.038466 0.002016 0.015906 0.003829 0.037421
None 0.003623 0.010194 0.004339 0.006633 0.006732 0.004197 0.004053 0.012691 0.004992 0.005605 0.004332 0.010948
Nuclear Periphery 0.000983 0.000828 0.001529 0.004020 0.000832 0.001348 0.001741 0.001613 0.000917 0.001226 0.001533 0.002306
Nuclear Periphery Foci 0.000380 0.000880 0.000181 0.003498 0.000812 0.000400 0.003538 0.000225 0.000571 0.000513 0.000257 0.001167
Nucleolus 0.001340 0.004146 0.000537 0.000683 0.002052 0.002399 0.001471 0.000904 0.001653 0.002809 0.000558 0.000841
Nucleus 0.425875* 0.314772* 0.837694* 0.564318* 0.444507* 0.387092* 0.861845* 0.501480* 0.434082* 0.370112* 0.838239* 0.519562*
Peroxisomes 0.004065 0.016221 0.000149 0.000461 0.002800 0.007417 0.000346 0.000549 0.003508 0.009484 0.000153 0.000524
Vacuole 0.017600 0.053208 0.009287 0.055981 0.014137 0.030503 0.058670 0.027776 0.016075 0.035834 0.010403 0.035893
Vacuole Periphery 0.000380 0.001651 0.000134 0.004819 0.000650 0.001236 0.000225 0.002433 0.000499 0.001334 0.000136 0.003120

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.22 -5.09 -12.17 0.15 -9.46 -3.45 -3.96 -3.41 -0.36 1.85 -4.89 -6.64 -5.47 -0.41 -3.65
Bud Neck -6.13 -1.97 -3.64 3.17 -1.61 -13.23 -0.70 -13.03 2.77 -10.41 -13.31 -3.37 -11.56 4.03 -4.85
Bud Site -6.73 -4.29 -6.51 -0.31 -4.18 -9.28 -2.78 -11.44 -1.60 -6.63 -11.21 -4.61 -12.96 -1.58 -6.60
Cell Periphery -1.65 -0.47 -5.74 1.13 -5.03 -0.51 -1.89 2.16 1.63 2.35 -1.94 0.50 -2.57 1.49 -2.33
Cytoplasm 7.02 42.69 25.01 12.85 -9.35 -0.64 14.49 16.75 19.88 -8.69 2.97 50.75 28.27 24.11 -23.41
Cytoplasmic Foci -7.98 31.21 30.35 24.84 -6.19 5.87 25.66 27.76 30.71 1.32 1.66 41.86 41.33 38.42 -4.51
Eisosomes -7.38 17.80 4.72 11.06 -16.92 4.04 -2.51 10.36 12.88 5.70 1.61 17.98 14.35 15.63 -9.56
Endoplasmic Reticulum -7.42 -14.43 -19.05 -15.46 -13.71 -2.80 -5.69 -16.08 -11.78 5.08 -6.40 -15.56 -22.70 -18.53 -5.03
Endosome -8.35 8.78 1.62 8.86 -6.24 -5.96 -0.25 2.67 9.39 1.82 -9.94 9.01 3.81 12.63 -4.70
Golgi -4.95 4.44 -1.01 2.38 -2.11 -4.21 3.73 -1.45 2.42 -2.49 -5.34 6.30 -1.63 3.10 -3.48
Lipid Particles -1.37 12.36 9.99 8.56 -11.60 3.98 3.84 9.48 13.23 3.21 3.44 15.04 14.31 15.51 -5.09
Mitochondria -3.37 3.96 1.16 4.20 -3.26 -8.30 3.45 -2.62 6.99 -7.82 -7.38 4.51 -0.02 8.17 -7.04
Mitotic Spindle -3.37 -1.54 -6.25 -2.35 -5.67 -7.81 -1.09 -10.06 -2.61 0.01 -8.62 -2.09 -11.93 -3.35 -9.81
None -3.21 -0.89 -2.19 2.55 -1.49 3.12 1.21 -4.49 -13.25 -3.29 -0.78 1.66 -7.73 -5.97 -10.62
Nuclear Periphery 1.20 -7.77 -8.46 -9.01 -6.25 -3.40 -4.90 -17.22 -10.00 0.17 -2.64 -11.18 -16.98 -12.96 -10.22
Nuclear Periphery Foci -2.20 0.86 -4.18 -3.15 -4.60 1.01 -1.79 1.35 1.79 2.28 0.13 1.36 -1.80 -2.71 -4.27
Nucleolus -4.82 3.76 3.60 7.32 -0.68 -0.96 1.86 2.57 5.55 0.45 -3.48 4.94 3.73 8.37 -2.90
Nucleus 9.59 -53.40 -16.44 -21.89 23.20 6.15 -5.88 -17.80 -27.56 2.26 9.30 -60.47 -25.84 -34.44 34.34
Peroxisomes -6.13 6.56 6.32 8.57 -2.37 -6.24 7.32 7.75 11.03 0.81 -7.75 9.23 8.92 13.90 -2.02
Vacuole -11.34 2.57 -18.18 -9.57 -18.71 -10.50 -4.17 -15.67 -7.53 3.06 -14.68 -0.73 -23.23 -12.55 -20.39
Vacuole Periphery -7.93 4.78 -2.24 -0.39 -2.61 -1.78 1.48 -0.79 0.64 -2.36 -4.63 2.75 -2.28 0.21 -3.67
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
Localization
Cell Percentages cytoplasm (18%), nucleus (11%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YPL245W

YPL245W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl245w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available