Standard name
Human Ortholog
Description Adaptor protein required for structural integrity of the SAGA complex; a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0.05 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.07 0 0.06 0.05 0 0 0 0 0 0.08 0.11 0.18 0.06 0.1 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0.18 0.17 0.09 0.23 0.32 0.26 0.35 0.35 0.27 0.32 0 0 0 0.09 0.06 0.05 0 0 0 0 0 0
Nucleus 0.89 0.93 0.88 0.78 0.89 0.8 0.74 0.8 0.76 0.75 0.73 0.72 0.87 0.79 0.73 0.87 0.86 0.83 0.81 0.87 0.75 0.74 0.68 0.57
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.22 0.13 0.17 0.09 0.2 0.22 0.24 0.32 0.33 0.35 0.37 0.23 0.14 0.1 0.09 0.05 0.09 0.09 0.05 0.1 0.14 0.16 0.19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
Bud 1 0 0 1 0 0 2 1 3 2 1 2 0 0 1 0 0 0 5 1 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud Site 2 0 2 0 1 9 5 9 15 19 4 14 0 3 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10 2 7 12 9 19 20 19 8 12 6 5 9 17 38 13 25 19 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 2 4 8 0 0 0 0 0 0
Endosome 0 0 1 0 1 0 0 0 0 0 0 0 0 2 3 2 2 2 5 0 3 0 2 4
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2 3 3 0 1 1 1 0
Mitochondria 48 2 27 27 22 76 127 124 93 124 58 81 4 5 4 18 14 14 2 0 1 0 0 2
Nucleus 261 83 134 127 229 267 295 384 202 263 154 184 92 120 151 175 217 215 247 84 120 44 72 81
Nuclear Periphery 0 0 0 3 1 5 9 4 1 2 2 3 0 1 4 2 6 1 0 0 0 0 0 0
Nucleolus 65 20 20 28 23 66 88 113 85 117 74 94 24 21 20 18 12 23 27 4 15 8 17 27
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 0 0 1 0 0 0 0 0 0
SpindlePole 1 0 3 1 0 0 3 3 0 0 0 0 0 2 3 4 5 6 2 0 2 0 3 2
Vac/Vac Membrane 8 2 0 2 4 5 6 1 1 2 1 3 1 2 3 6 4 11 8 5 9 1 3 16
Unique Cell Count 294 89 152 162 257 333 396 480 265 352 211 257 106 151 207 202 253 258 305 97 161 60 107 143
Labelled Cell Count 396 109 194 201 291 447 555 659 408 542 300 386 130 177 230 242 291 303 305 97 161 60 107 143


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 7.2 5.8 5.5 5.8 5.3 4.4 4.9 4.7 4.5 4.8 4.6 6.8 7.1 7.0 6.7 7.6 8.3 6.0 6.5 7.2
Std Deviation (1e-4) 1.1 1.2 1.7 1.6 1.4 1.9 1.4 1.6 1.4 1.5 1.9 1.4 2.0 2.6 2.8 1.6 2.2 2.4 1.2 1.5 1.5
Intensity Change (Log2) – – – -0.08 -0.01 -0.14 -0.41 -0.24 -0.3 -0.38 -0.29 -0.35 0.23 0.28 0.26 0.19 0.38 0.51 0.05 0.16 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 -0.6 0.5 0.2 -0.3 -0.8 -0.6 -0.9 0 1.3 2.1 3.9 0.7 1.9 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.3 -2.8 1.3 0 2.0 0 0 0 0 -3.4 -4.1 -5.3 -2.5 -4.0 -4.0
Nucleus -2.3 0.3 -2.2 -3.5 -2.3 -3.0 -3.4 -3.5 -3.9 -0.3 -2.1 -3.5 -0.4 -0.7 -1.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.0 -1.3 1.8 0 2.7 0 0 0 0 2.0 0.2 -1.0 -1.3 -3.0 -1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2363 2.5561 2.2963 1.7088 1.76 2.1152 3.3756 2.8085 3.3337 2.2634 2.8563 3.1102 4.5356 4.0075 4.5056 3.77 3.863 4.09
Actin 0.0072 0.0014 0.0009 0.0008 0.003 0.0092 0.0062 0 0.0292 0.0302 0.0055 0.0081 0.0742 0 0.0258 0 0.0172 0.0137
Bud 0.0003 0.0001 0.0002 0.0001 0.0004 0.0001 0.0004 0 0.0004 0.0002 0.0012 0.0004 0.0012 0 0.0004 0 0.0002 0.0001
Bud Neck 0.0008 0.0001 0.0004 0.0001 0.0021 0.0013 0.0009 0.0001 0.0002 0.0003 0.0032 0.0075 0.0043 0.0001 0.0007 0 0.0004 0.0009
Bud Periphery 0.0008 0.0001 0.0006 0.0001 0.0014 0.0004 0.0006 0 0.0004 0.0002 0.0047 0.0006 0.0024 0 0.0013 0 0.0003 0.0001
Bud Site 0.0011 0.0003 0.0005 0.0003 0.0013 0.0002 0.0025 0.0001 0.0007 0.0003 0.0025 0.0142 0.0049 0.0001 0.0057 0 0.0004 0.0001
Cell Periphery 0.0002 0 0.0001 0 0.0005 0.0001 0.0006 0 0.0001 0 0.001 0.0001 0.0007 0 0.0008 0 0.0001 0
Cytoplasm 0.0096 0.0002 0.0086 0.0003 0.0172 0.0002 0.0061 0.0001 0.0195 0.0002 0.0136 0.0015 0.0037 0.0001 0.001 0 0.0037 0.0001
Cytoplasmic Foci 0.0247 0.0011 0.0045 0.0047 0.003 0.0022 0.0329 0 0.015 0.0067 0.0082 0.0039 0.0461 0 0.0195 0 0.0042 0.0005
Eisosomes 0.0002 0 0 0 0.0002 0 0.0001 0 0.0003 0 0.0002 0.0001 0.0007 0 0.0012 0 0.0002 0
Endoplasmic Reticulum 0.0077 0 0.0012 0 0.0036 0.0003 0.0021 0 0.0047 0.0001 0.0052 0.0027 0.0148 0 0.0004 0 0.0035 0.0002
Endosome 0.0319 0.0002 0.0055 0.0035 0.0118 0.0043 0.0766 0 0.0251 0.0026 0.0708 0.0027 0.0249 0 0.0009 0 0.013 0.0012
Golgi 0.0082 0.0007 0.0004 0.0052 0.003 0.0008 0.0099 0 0.0083 0.009 0.0101 0.0008 0.0125 0 0.001 0 0.0064 0.0018
Lipid Particles 0.0293 0.0007 0.0011 0.0022 0.022 0.0008 0.0285 0 0.0149 0.0018 0.0074 0.0007 0.0115 0 0.0026 0 0.045 0.0007
Mitochondria 0.0121 0.001 0.0022 0.0062 0.0184 0.0025 0.0031 0.0001 0.0051 0.002 0.0187 0.0021 0.0155 0.0001 0.0014 0.0002 0.0079 0.0003
None 0.0178 0.0004 0.0011 0.0011 0.0124 0.0001 0.0037 0 0.0247 0.0001 0.1097 0.0041 0.0031 0 0.0017 0 0.0015 0.0001
Nuclear Periphery 0.0344 0.0007 0.0113 0.0007 0.0332 0.0023 0.0273 0.0015 0.0122 0.0023 0.0186 0.0104 0.0072 0.0003 0.0014 0.0033 0.0376 0.0006
Nucleolus 0.0487 0.0467 0.0392 0.0168 0.2223 0.087 0.0484 0.0206 0.022 0.012 0.1233 0.0634 0.0199 0.0331 0.0221 0.0175 0.0681 0.0683
Nucleus 0.7208 0.9346 0.8964 0.9374 0.5822 0.8841 0.6916 0.9761 0.7985 0.9221 0.5513 0.8214 0.7118 0.9653 0.8851 0.9783 0.771 0.8943
Peroxisomes 0.0112 0.0094 0.0004 0.0182 0.0009 0.0003 0.0069 0 0.0053 0.0089 0.0018 0.0008 0.0165 0 0.01 0 0.012 0.0002
Punctate Nuclear 0.0278 0.0024 0.0229 0.002 0.0519 0.0025 0.0413 0.001 0.0104 0.0007 0.0113 0.0534 0.0189 0.0009 0.0166 0.0004 0.0056 0.0165
Vacuole 0.0026 0.0001 0.0019 0.0001 0.0045 0.0006 0.008 0.0001 0.0017 0.0001 0.0251 0.0005 0.0036 0 0.0002 0 0.0006 0.0001
Vacuole Periphery 0.0026 0 0.0006 0.0001 0.0048 0.0006 0.0023 0.0001 0.0011 0.0001 0.0064 0.0004 0.0015 0 0.0001 0.0001 0.0011 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.043 17.4818 9.9786 20.306 17.0659 20.2968 16.996 16.7479 17.8438 24.0328
Translational Efficiency 1.0498 0.7715 1.5364 0.9903 1.0861 0.7223 0.7691 0.8671 0.9667 0.6663

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1852 1471 212 1213 1356 1397 1437 1729 3208 2868 1649 2942

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 688.82 879.02 1081.78 935.76 818.86 845.53 1026.02 947.16 743.79 862.71 1033.19 942.46
Standard Deviation 97.15 163.39 138.73 144.45 113.40 140.93 137.33 130.90 122.52 153.78 138.78 136.76
Intensity Change Log 2 — 0.351769 0.651208 0.442012 — 0.046239 0.325370 0.209991 — 0.193890 0.483408 0.320641

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000189 0.000700 0.001501 0.001136 0.000241 0.001160 0.000754 0.001032 0.000211 0.000924 0.000850 0.001075
Bud Neck 0.005205 0.012787 0.017092 0.028432 0.005609 0.019201 0.032950 0.035632 0.005376 0.015911 0.030911 0.032663
Bud Site 0.000639 0.001312 0.002033 0.008186 0.000415 0.003696 0.002148 0.008787 0.000544 0.002474 0.002133 0.008539
Cell Periphery 0.000233 0.000103 0.000051 0.000123 0.000131 0.000178 0.000061 0.000278 0.000190 0.000140 0.000060 0.000214
Cytoplasm 0.007330 0.003253 0.000595 0.004817 0.002897 0.003596 0.001227 0.020859 0.005457 0.003420 0.001146 0.014245
Cytoplasmic Foci 0.001000 0.000790 0.000048 0.001554 0.000943 0.002464 0.000109 0.003693 0.000976 0.001605 0.000101 0.002811
Eisosomes 0.000022 0.000033 0.000065 0.000034 0.000024 0.000044 0.000036 0.000038 0.000023 0.000038 0.000039 0.000036
Endoplasmic Reticulum 0.002669 0.003104 0.003918 0.004945 0.002042 0.005193 0.002667 0.004038 0.002404 0.004121 0.002828 0.004412
Endosome 0.000549 0.000611 0.000133 0.002351 0.000188 0.002562 0.000257 0.003547 0.000396 0.001561 0.000241 0.003054
Golgi 0.000116 0.000220 0.000160 0.002401 0.000060 0.001689 0.000102 0.003715 0.000092 0.000936 0.000110 0.003173
Lipid Particles 0.000469 0.000367 0.000089 0.000351 0.000102 0.001144 0.000070 0.000195 0.000314 0.000745 0.000072 0.000260
Mitochondria 0.001425 0.001488 0.001395 0.002819 0.000474 0.011125 0.001549 0.003559 0.001023 0.006182 0.001529 0.003254
Mitotic Spindle 0.000530 0.002298 0.001024 0.020381 0.001682 0.022244 0.003907 0.026804 0.001017 0.012014 0.003536 0.024155
None 0.015908 0.005440 0.001586 0.004480 0.006059 0.005774 0.002595 0.009255 0.011745 0.005602 0.002465 0.007286
Nuclear Periphery 0.000672 0.000258 0.000167 0.000961 0.000248 0.001158 0.000378 0.001355 0.000493 0.000696 0.000351 0.001193
Nuclear Periphery Foci 0.000919 0.000431 0.000103 0.000476 0.000297 0.001175 0.000402 0.000711 0.000656 0.000793 0.000364 0.000614
Nucleolus 0.043441 0.056273 0.042191 0.013603 0.054604 0.052932 0.021354 0.013229 0.048159 0.054645 0.024033 0.013383
Nucleus 0.917389* 0.908605* 0.925174* 0.899056* 0.923481* 0.856347* 0.927921* 0.855141* 0.919964* 0.883150* 0.927568* 0.873247*
Peroxisomes 0.000399 0.000932 0.001443 0.000420 0.000239 0.002495 0.000342 0.000352 0.000332 0.001694 0.000484 0.000380
Vacuole 0.000560 0.000592 0.001048 0.002652 0.000139 0.003597 0.000975 0.006064 0.000382 0.002056 0.000984 0.004657
Vacuole Periphery 0.000334 0.000404 0.000185 0.000822 0.000126 0.002226 0.000198 0.001715 0.000246 0.001291 0.000196 0.001347

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.97 -13.28 -15.03 -6.54 1.99 -10.93 -22.26 -14.16 0.28 -3.33 -12.83 -26.22 -20.63 -4.24 -4.01
Bud Neck -8.73 -7.11 -16.52 -11.22 -5.30 -11.39 -20.80 -17.44 -8.15 1.12 -14.35 -22.14 -23.87 -13.65 0.57
Bud Site -3.41 -6.09 -5.40 -4.69 -3.57 -6.35 -10.11 -7.28 -3.02 -4.60 -7.09 -10.68 -9.00 -5.68 -5.68
Cell Periphery 3.96 6.21 4.07 -0.49 -5.77 -0.87 1.82 -1.17 -0.28 -4.07 1.77 5.55 1.14 -0.72 -5.09
Cytoplasm 2.60 5.99 1.97 -1.17 -6.33 -0.62 2.25 -8.26 -8.01 -10.41 1.99 5.93 -5.73 -7.65 -11.48
Cytoplasmic Foci 0.38 3.87 0.48 0.06 -2.43 -1.48 1.37 -1.07 0.68 -4.45 -1.19 2.93 -0.98 0.34 -5.01
Eisosomes -6.58 -10.43 -9.02 -2.73 6.18 -9.21 -10.34 -6.83 1.91 0.54 -11.40 -15.35 -10.74 -0.23 2.67
Endoplasmic Reticulum -2.12 -4.06 -9.99 -8.70 -5.12 -10.06 -6.79 -11.94 1.40 -8.12 -9.06 -5.43 -14.55 -4.50 -11.38
Endosome -0.24 4.58 -2.04 -1.54 -3.03 -4.70 -0.78 -4.23 1.05 -4.02 -3.92 1.99 -4.37 -0.33 -4.87
Golgi -2.02 -2.53 -1.85 -1.67 -1.72 -3.41 -10.77 -3.12 -0.11 -2.95 -3.63 -3.98 -3.55 -1.36 -3.38
Lipid Particles -0.13 3.43 1.21 0.68 -1.59 -3.64 1.30 -2.90 3.19 -3.70 -2.31 3.75 0.78 2.62 -3.26
Mitochondria -0.21 -0.38 -3.69 -4.67 -5.78 -6.15 -8.71 -8.03 4.43 -3.30 -5.84 -3.36 -6.81 3.33 -4.67
Mitotic Spindle -2.68 -1.06 -6.38 -5.53 -4.12 -6.37 -2.64 -8.75 -1.75 -6.71 -6.93 -3.49 -11.05 -5.16 -7.54
None 5.28 8.09 6.45 0.95 -5.42 0.51 3.21 -1.14 -2.23 -7.21 4.99 8.23 4.53 -1.21 -7.98
Nuclear Periphery 3.31 3.91 -5.93 -10.85 -11.62 -4.22 -5.95 -8.09 -1.75 -6.17 -1.70 -0.30 -8.88 -6.11 -9.56
Nuclear Periphery Foci 0.95 2.19 1.21 0.30 -2.98 -2.91 0.38 -1.13 2.40 -1.33 -0.62 1.61 0.75 1.96 -1.50
Nucleolus -4.65 -0.60 11.63 15.11 5.79 0.37 10.40 16.07 15.97 5.83 -3.24 9.47 19.97 22.40 7.40
Nucleus 2.11 0.51 4.48 2.87 2.80 9.99 2.41 10.45 -0.55 8.59 9.06 2.05 10.82 2.03 8.39
Peroxisomes -2.08 -4.77 0.13 2.26 5.22 -4.13 -2.90 -1.91 4.02 1.57 -4.56 -2.54 -0.00 4.64 3.63
Vacuole -0.30 -2.84 -6.05 -6.01 -5.03 -4.25 -4.95 -7.53 -2.47 -6.09 -4.10 -4.00 -9.34 -5.23 -7.57
Vacuole Periphery -0.75 1.96 -1.31 -0.84 -2.32 -4.28 -2.84 -3.72 2.51 -3.32 -4.27 1.04 -3.57 1.96 -4.00
Standard name
Human Ortholog
Description Adaptor protein required for structural integrity of the SAGA complex; a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Hfi1

Hfi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hfi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available