Standard name | |
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Human Ortholog | |
Description | Protein required for the spindle pole body (SPB) duplication; localizes at the cytoplasmic side of the central plaque periphery of the SPB; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 6 | 4 | 2 | 3 | 14 | 5 | 2 | 2 | 0 | 2 | 3 | 4 | 3 | 0 | 3 | 3 | 1 | 0 | 4 | 4 | 2 | 5 | 10 |
Bud | 7 | 9 | 34 | 33 | 70 | 71 | 100 | 66 | 75 | 68 | 107 | 152 | 3 | 1 | 3 | 12 | 8 | 4 | 1 | 9 | 4 | 12 | 10 | 22 |
Bud Neck | 1 | 1 | 4 | 4 | 7 | 10 | 11 | 5 | 3 | 13 | 6 | 7 | 0 | 1 | 1 | 1 | 6 | 0 | 0 | 5 | 1 | 1 | 4 | 10 |
Bud Site | 14 | 4 | 15 | 24 | 32 | 56 | 83 | 39 | 80 | 52 | 98 | 115 | 6 | 5 | 0 | 7 | 9 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 25 | 27 | 20 | 45 | 43 | 40 | 38 | 23 | 44 | 25 | 39 | 55 | 84 | 106 | 40 | 38 | 29 | 2 | 8 | 4 | 4 | 7 | 5 |
Endoplasmic Reticulum | 0 | 1 | 1 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 1 | 9 | 11 | 11 | 0 | 1 | 2 | 0 | 3 | 9 |
Endosome | 128 | 146 | 79 | 56 | 87 | 17 | 14 | 31 | 37 | 22 | 51 | 42 | 117 | 254 | 313 | 114 | 95 | 102 | 74 | 108 | 90 | 68 | 52 | 72 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 2 | 4 | 2 | 2 | 2 |
Mitochondria | 31 | 26 | 46 | 42 | 85 | 181 | 272 | 237 | 214 | 251 | 332 | 386 | 2 | 1 | 3 | 34 | 23 | 18 | 6 | 8 | 12 | 16 | 21 | 30 |
Nucleus | 1 | 3 | 1 | 5 | 13 | 3 | 25 | 23 | 22 | 21 | 15 | 48 | 3 | 4 | 4 | 5 | 3 | 3 | 1 | 4 | 2 | 2 | 1 | 1 |
Nuclear Periphery | 2 | 6 | 0 | 0 | 3 | 1 | 4 | 1 | 0 | 8 | 3 | 3 | 2 | 4 | 3 | 1 | 0 | 1 | 10 | 8 | 5 | 5 | 7 | 9 |
Nucleolus | 144 | 117 | 68 | 58 | 79 | 113 | 148 | 164 | 146 | 115 | 194 | 263 | 114 | 106 | 137 | 92 | 76 | 66 | 1 | 8 | 2 | 0 | 1 | 1 |
Peroxisomes | 42 | 39 | 32 | 33 | 47 | 21 | 43 | 29 | 55 | 34 | 86 | 80 | 20 | 71 | 95 | 11 | 16 | 22 | 10 | 12 | 14 | 29 | 24 | 33 |
SpindlePole | 314 | 313 | 435 | 375 | 498 | 413 | 583 | 462 | 415 | 403 | 564 | 766 | 442 | 551 | 459 | 315 | 242 | 224 | 322 | 344 | 405 | 264 | 241 | 282 |
Vac/Vac Membrane | 23 | 39 | 5 | 6 | 7 | 6 | 4 | 1 | 8 | 9 | 8 | 9 | 29 | 36 | 68 | 28 | 19 | 19 | 4 | 7 | 5 | 8 | 6 | 11 |
Unique Cell Count | 436 | 500 | 536 | 457 | 664 | 631 | 883 | 756 | 660 | 685 | 944 | 1243 | 570 | 754 | 766 | 441 | 346 | 321 | 451 | 550 | 573 | 427 | 403 | 518 |
Labelled Cell Count | 722 | 736 | 751 | 660 | 976 | 950 | 1333 | 1098 | 1081 | 1040 | 1491 | 1913 | 800 | 1122 | 1194 | 672 | 550 | 507 | 451 | 550 | 573 | 427 | 403 | 518 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 6.0 | 4.4 | 4.2 | 4.0 | 3.9 | 3.5 | 3.7 | 3.6 | 3.3 | 3.5 | 3.6 | 6.7 | 6.6 | 6.7 | 6.6 | 6.0 | 6.4 | 6.9 | 6.8 | 7.0 |
Std Deviation (1e-4) | 0.8 | 1.1 | 0.9 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.0 | 1.0 | 0.8 | 1.0 | 1.2 | 1.6 | 1.4 | 1.1 | 1.1 | 1.2 | 0.9 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.13 | -0.17 | -0.34 | -0.26 | -0.28 | -0.39 | -0.33 | -0.28 | 0.62 | 0.59 | 0.61 | 0.6 | 0.47 | 0.55 | 0.65 | 0.64 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.4 | -6.8 | -6.4 | -2.7 | -2.7 | -3.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | -3.1 | -4.7 | -1.3 | -0.2 | -1.5 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | 1.3 | 1.3 | -0.4 | 0 | -1.3 | 1.0 | -2.4 | -1.9 | 2.9 | 3.9 | 5.2 | 2.5 | 3.3 | 2.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 | 7.7 | 10.1 | 4.3 | 4.6 | 5.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.7 | -8.0 | -7.6 | -0.5 | -1.0 | -1.6 |
Nucleus | 0 | 2.8 | 0 | 3.6 | 3.7 | 3.9 | 3.8 | 2.5 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0.7 | 2.5 | 3.4 | 3.6 | 3.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.9 | 2.3 | 3.8 | -2.6 | -0.9 | 0.5 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.5 | -3.4 | -8.1 | -3.6 | -3.9 | -3.8 |
Vacuole | 0.6 | 0.2 | 0 | 0 | 0 | 0.5 | 0.6 | 0 | 0 | 4.0 | 3.9 | 6.1 | 4.7 | 4.1 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.9796 | 1.4103 | 1.6516 | 1.3321 | 1.9851 | 1.4526 | 3.6342 | 3.8373 | 4.0522 | 3.6677 | 3.7939 | 3.7016 | 0.171 | 0.795 | 0.6982 | 0.7703 | 0.7197 | 0.489 |
Actin | 0.0071 | 0.0026 | 0.0024 | 0.0016 | 0.006 | 0.0005 | 0.0113 | 0.0007 | 0.0117 | 0.0002 | 0.0012 | 0.0045 | 0.0014 | 0.0003 | 0.0002 | 0.0009 | 0.0004 | 0.0002 |
Bud | 0.003 | 0.0047 | 0.0024 | 0.0031 | 0.0016 | 0.0009 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Neck | 0.0021 | 0.0017 | 0.0068 | 0.0236 | 0.0013 | 0.0013 | 0.0008 | 0.0006 | 0.005 | 0.005 | 0.0002 | 0.001 | 0.0007 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0003 |
Bud Periphery | 0.0016 | 0.0014 | 0.0012 | 0.0014 | 0.0008 | 0.0003 | 0.0003 | 0.0001 | 0.0009 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0058 | 0.0133 | 0.0055 | 0.0032 | 0.0014 | 0.0004 | 0.0013 | 0.0023 | 0.005 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Cell Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0058 | 0.0158 | 0.0043 | 0.0242 | 0.0079 | 0.0082 | 0.0033 | 0.0142 | 0.001 | 0.0005 | 0.0005 | 0.0031 | 0.0027 | 0.0025 | 0.0004 | 0.0009 | 0.0103 | 0.0014 |
Cytoplasmic Foci | 0.1008 | 0.0486 | 0.0671 | 0.0722 | 0.1336 | 0.0332 | 0.0721 | 0.0492 | 0.0633 | 0.0596 | 0.0795 | 0.0265 | 0.0667 | 0.0737 | 0.1002 | 0.0687 | 0.1993 | 0.0525 |
Eisosomes | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0015 | 0.002 | 0.0005 | 0.001 | 0.0005 | 0.0002 | 0.0011 | 0.0016 | 0.0012 | 0.0001 | 0.0007 | 0.0004 | 0.0009 | 0.0005 | 0.0001 | 0.0002 | 0.0015 | 0.0003 |
Endosome | 0.0486 | 0.0225 | 0.0121 | 0.0373 | 0.0689 | 0.005 | 0.0224 | 0.0218 | 0.0066 | 0.004 | 0.0128 | 0.005 | 0.007 | 0.0084 | 0.0058 | 0.0018 | 0.0173 | 0.0046 |
Golgi | 0.0173 | 0.0036 | 0.0042 | 0.0306 | 0.0536 | 0.0015 | 0.0061 | 0.0028 | 0.0041 | 0.0023 | 0.0123 | 0.0015 | 0.0023 | 0.0022 | 0.0025 | 0.0011 | 0.005 | 0.0015 |
Lipid Particles | 0.0725 | 0.0388 | 0.0586 | 0.0474 | 0.1252 | 0.0346 | 0.0594 | 0.0382 | 0.0535 | 0.0232 | 0.0591 | 0.0207 | 0.044 | 0.0492 | 0.0588 | 0.0159 | 0.2001 | 0.0228 |
Mitochondria | 0.0299 | 0.0014 | 0.0088 | 0.0129 | 0.0825 | 0.0003 | 0.004 | 0.0017 | 0.0009 | 0 | 0.0044 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
None | 0.017 | 0.011 | 0.0018 | 0.0075 | 0.0086 | 0.002 | 0.0141 | 0.0076 | 0.0013 | 0.0002 | 0.0006 | 0.0007 | 0.0023 | 0.0004 | 0.0003 | 0.0006 | 0.0047 | 0.0012 |
Nuclear Periphery | 0.0155 | 0.0123 | 0.0192 | 0.0182 | 0.0086 | 0.0073 | 0.0118 | 0.016 | 0.0152 | 0.0062 | 0.0187 | 0.0063 | 0.0168 | 0.0171 | 0.004 | 0.0183 | 0.0139 | 0.0074 |
Nucleolus | 0.0197 | 0.0276 | 0.0251 | 0.005 | 0.0287 | 0.0089 | 0.0024 | 0.0108 | 0.0025 | 0.0024 | 0.0023 | 0.0016 | 0.0021 | 0.0077 | 0.0042 | 0.0011 | 0.002 | 0.0028 |
Nucleus | 0.0211 | 0.0267 | 0.0137 | 0.0197 | 0.0096 | 0.0105 | 0.0057 | 0.0094 | 0.0018 | 0.0011 | 0.001 | 0.0012 | 0.0031 | 0.0192 | 0.0033 | 0.0069 | 0.0012 | 0.0062 |
Peroxisomes | 0.025 | 0.0195 | 0.0215 | 0.0201 | 0.083 | 0.0089 | 0.0339 | 0.0099 | 0.0172 | 0.0049 | 0.009 | 0.0038 | 0.0056 | 0.0125 | 0.0176 | 0.0038 | 0.0122 | 0.0046 |
Punctate Nuclear | 0.5958 | 0.7421 | 0.7418 | 0.6673 | 0.3712 | 0.8751 | 0.7482 | 0.8109 | 0.8067 | 0.8896 | 0.7955 | 0.9226 | 0.8435 | 0.8051 | 0.8015 | 0.8791 | 0.53 | 0.8938 |
Vacuole | 0.0067 | 0.0036 | 0.0016 | 0.0018 | 0.0044 | 0.0005 | 0.0008 | 0.0015 | 0.0007 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0007 | 0.0002 |
Vacuole Periphery | 0.0025 | 0.0006 | 0.0016 | 0.0015 | 0.0024 | 0.0002 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0007 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.6228 | 6.8143 | 2.5283 | 10.1014 | 19.149 | 5.714 | 7.9665 | 8.4286 | 9.7096 | 15.4002 |
Translational Efficiency | 1.1105 | 0.706 | 1.2918 | 0.5669 | 0.5074 | 1.503 | 0.8027 | 0.5312 | 0.6929 | 0.6557 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1770 | 2094 | 284 | 2153 | 1613 | 2491 | 1515 | 1623 | 3383 | 4585 | 1799 | 3776 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 667.64 | 800.13 | 902.63 | 904.39 | 752.45 | 772.48 | 800.13 | 941.04 | 708.08 | 785.11 | 816.31 | 920.14 |
Standard Deviation | 90.77 | 115.93 | 106.20 | 121.77 | 85.25 | 304.49 | 120.01 | 132.64 | 97.83 | 238.11 | 123.71 | 127.85 |
Intensity Change Log 2 | — | 0.261164 | 0.435064 | 0.437875 | — | 0.037902 | 0.088639 | 0.322660 | — | 0.147179 | 0.261893 | 0.377975 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000168 | 0.000178 | 0.000409 | 0.000378 | 0.000156 | 0.000317 | 0.000185 | 0.000618 | 0.000162 | 0.000253 | 0.000220 | 0.000481 |
Bud Neck | 0.001549 | 0.003144 | 0.002206 | 0.006986 | 0.001681 | 0.001771 | 0.001635 | 0.003161 | 0.001612 | 0.002398 | 0.001725 | 0.005342 |
Bud Site | 0.001467 | 0.002343 | 0.001452 | 0.011425 | 0.000816 | 0.001813 | 0.001578 | 0.010252 | 0.001157 | 0.002055 | 0.001558 | 0.010921 |
Cell Periphery | 0.000131 | 0.000102 | 0.000067 | 0.000078 | 0.000081 | 0.000106 | 0.000066 | 0.000062 | 0.000107 | 0.000104 | 0.000066 | 0.000071 |
Cytoplasm | 0.012869 | 0.022095 | 0.001563 | 0.038627 | 0.012286 | 0.014847 | 0.011534 | 0.009541 | 0.012591 | 0.018157 | 0.009960 | 0.026125 |
Cytoplasmic Foci | 0.133872 | 0.094668 | 0.005078 | 0.017134 | 0.080202 | 0.104559 | 0.008645 | 0.027781 | 0.108282 | 0.100041 | 0.008082 | 0.021711 |
Eisosomes | 0.000053 | 0.000042 | 0.000036 | 0.000023 | 0.000060 | 0.000037 | 0.000023 | 0.000015 | 0.000056 | 0.000039 | 0.000025 | 0.000020 |
Endoplasmic Reticulum | 0.001292 | 0.001536 | 0.004310 | 0.001523 | 0.001137 | 0.000950 | 0.001550 | 0.000946 | 0.001218 | 0.001218 | 0.001986 | 0.001275 |
Endosome | 0.027983 | 0.023882 | 0.004877 | 0.015275 | 0.025195 | 0.037276 | 0.007051 | 0.022956 | 0.026654 | 0.031159 | 0.006708 | 0.018577 |
Golgi | 0.011742 | 0.009336 | 0.000586 | 0.003792 | 0.008370 | 0.010849 | 0.000866 | 0.003282 | 0.010134 | 0.010158 | 0.000822 | 0.003573 |
Lipid Particles | 0.223969* | 0.096815 | 0.008600 | 0.009231 | 0.168349* | 0.159287* | 0.008100 | 0.025281 | 0.197449* | 0.130756* | 0.008179 | 0.016130 |
Mitochondria | 0.003714 | 0.001973 | 0.000413 | 0.004110 | 0.002575 | 0.003363 | 0.000485 | 0.002202 | 0.003171 | 0.002728 | 0.000473 | 0.003290 |
Mitotic Spindle | 0.043352 | 0.180116* | 0.449741* | 0.625806* | 0.112925* | 0.183120* | 0.419983* | 0.609989* | 0.076524 | 0.181748* | 0.424681* | 0.619007* |
None | 0.008945 | 0.005357 | 0.000558 | 0.003746 | 0.003454 | 0.006971 | 0.003014 | 0.000983 | 0.006327 | 0.006234 | 0.002626 | 0.002558 |
Nuclear Periphery | 0.002161 | 0.002775 | 0.007286 | 0.007063 | 0.003145 | 0.003913 | 0.004088 | 0.004691 | 0.002630 | 0.003393 | 0.004593 | 0.006044 |
Nuclear Periphery Foci | 0.473787* | 0.502353* | 0.462841* | 0.163549 | 0.510721* | 0.428087* | 0.487398* | 0.254031* | 0.491397* | 0.462005* | 0.483521* | 0.202440* |
Nucleolus | 0.024100 | 0.025822 | 0.014117 | 0.027009 | 0.038359 | 0.015958 | 0.023900 | 0.004081 | 0.030898 | 0.020463 | 0.022356 | 0.017154 |
Nucleus | 0.005106 | 0.011818 | 0.027012 | 0.043559 | 0.007680 | 0.007708 | 0.014912 | 0.004071 | 0.006334 | 0.009585 | 0.016822 | 0.026586 |
Peroxisomes | 0.022601 | 0.013157 | 0.004415 | 0.005313 | 0.021292 | 0.016936 | 0.002273 | 0.006812 | 0.021977 | 0.015210 | 0.002611 | 0.005957 |
Vacuole | 0.000908 | 0.002047 | 0.004277 | 0.013161 | 0.001080 | 0.001661 | 0.002596 | 0.007833 | 0.000990 | 0.001837 | 0.002862 | 0.010871 |
Vacuole Periphery | 0.000234 | 0.000441 | 0.000156 | 0.002214 | 0.000437 | 0.000472 | 0.000120 | 0.001412 | 0.000331 | 0.000458 | 0.000125 | 0.001869 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.61 | -4.20 | -6.48 | -6.73 | 0.98 | -0.89 | -4.92 | -2.03 | -1.56 | -1.36 | -1.00 | -6.04 | -3.94 | -3.13 | -1.97 |
Bud Neck | -3.17 | -4.59 | -12.71 | -10.23 | -6.61 | -0.02 | -2.76 | -3.96 | -4.01 | -2.41 | -2.75 | -4.75 | -12.89 | -11.33 | -9.61 |
Bud Site | -2.95 | -2.90 | -9.03 | -8.42 | -5.75 | -3.08 | -5.26 | -6.35 | -5.90 | -4.97 | -3.92 | -5.42 | -11.00 | -10.26 | -8.63 |
Cell Periphery | 1.85 | 2.92 | 3.31 | 2.97 | -0.05 | -0.65 | 1.78 | 2.74 | 2.79 | 1.18 | 1.42 | 3.56 | 4.25 | 3.74 | 0.36 |
Cytoplasm | -2.79 | 5.71 | -11.58 | -9.36 | -14.92 | -1.15 | -1.89 | -2.08 | -1.16 | -0.16 | -2.69 | -0.98 | -11.20 | -9.29 | -9.04 |
Cytoplasmic Foci | 6.57 | 22.92 | 22.93 | 19.18 | -3.42 | -3.16 | 18.90 | 13.16 | 18.67 | -8.24 | 2.95 | 30.41 | 26.17 | 27.45 | -8.25 |
Eisosomes | 4.29 | 3.15 | 13.88 | 10.53 | 2.58 | 10.08 | 13.80 | 22.17 | 16.93 | 5.94 | 10.29 | 14.29 | 24.75 | 17.83 | 3.95 |
Endoplasmic Reticulum | -0.66 | -3.28 | -2.47 | -2.72 | 2.64 | 2.51 | -3.48 | 0.46 | -1.19 | 3.35 | 0.27 | -4.19 | -2.30 | -3.57 | 2.97 |
Endosome | 3.08 | 13.58 | 7.17 | 4.31 | -6.71 | -4.15 | 10.26 | 0.25 | 3.80 | -7.86 | -1.20 | 14.87 | 5.37 | 6.45 | -8.68 |
Golgi | 2.51 | 14.82 | 10.44 | 9.04 | -3.32 | -1.26 | 13.27 | 7.62 | 8.07 | -1.85 | 1.30 | 19.01 | 12.89 | 11.97 | -3.16 |
Lipid Particles | 15.90 | 29.98 | 30.38 | 20.35 | 0.10 | 0.60 | 23.87 | 19.84 | 23.26 | -7.68 | 11.29 | 38.26 | 36.03 | 31.48 | -6.05 |
Mitochondria | 1.48 | 2.79 | -0.95 | -3.62 | -5.61 | -0.83 | 2.74 | -0.27 | 0.35 | -1.74 | 0.58 | 3.54 | -1.04 | -1.80 | -4.99 |
Mitotic Spindle | -21.11* | -16.95 | -58.37* | -36.00* | -5.85 | -9.62 | -25.81* | -42.10* | -35.00* | -12.80 | -21.57 | -35.04 | -70.14 | -50.36* | -15.09 |
None | 2.79 | 7.28 | 3.53 | 1.12 | -7.96 | -1.40 | 0.73 | 5.32 | 4.76 | 4.67 | 1.44 | 4.90 | 4.95 | 3.75 | -0.37 |
Nuclear Periphery | -1.08 | -3.19 | -7.49 | -7.07 | -0.76 | -1.36 | -1.91 | -2.94 | -2.09 | -0.80 | -1.86 | -3.27 | -7.76 | -6.70 | -2.68 |
Nuclear Periphery Foci | 0.14 | 2.58 | 33.15* | 34.71* | 12.54 | 6.82 | 4.36 | 21.71* | 16.85 | 16.27 | 5.08 | 4.61 | 39.29 | 36.86* | 25.88* |
Nucleolus | -0.70 | 2.00 | -4.85 | -4.29 | -5.44 | 7.94 | 2.85 | 13.52 | 9.45 | 9.73 | 5.45 | 1.69 | 3.49 | -1.56 | 1.12 |
Nucleus | -4.26 | -3.85 | -15.13 | -13.17 | -2.25 | 0.72 | -6.11 | 0.24 | -0.32 | 5.70 | -2.68 | -7.62 | -14.21 | -12.82 | -4.75 |
Peroxisomes | 5.03 | 6.57 | 11.75 | 8.48 | 0.03 | 2.06 | 11.42 | 8.38 | 7.70 | -3.08 | 4.85 | 15.82 | 14.33 | 11.63 | -2.85 |
Vacuole | -3.55 | -2.20 | -11.25 | -10.56 | -1.34 | -1.23 | -4.06 | -6.74 | -6.47 | -4.48 | -3.04 | -4.39 | -12.92 | -12.25 | -6.87 |
Vacuole Periphery | -1.69 | -1.04 | -3.71 | -3.37 | -3.56 | -0.30 | 1.59 | -2.02 | -1.95 | -2.34 | -1.19 | 1.45 | -4.10 | -3.81 | -4.35 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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