Standard name | |
---|---|
Human Ortholog | |
Description | Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 6 | 9 | 6 | 6 | 6 | 3 | 4 | 6 | 1 | 0 | 0 | 2 | 10 | 13 | 3 | 6 | 8 | 2 | 13 | 24 | 6 | 16 | 22 | 30 |
Bud | 16 | 20 | 5 | 6 | 10 | 8 | 17 | 35 | 10 | 28 | 20 | 10 | 6 | 9 | 7 | 6 | 10 | 5 | 5 | 13 | 5 | 14 | 11 | 16 |
Bud Neck | 5 | 12 | 19 | 7 | 16 | 14 | 19 | 42 | 12 | 17 | 10 | 7 | 13 | 16 | 13 | 5 | 9 | 6 | 12 | 21 | 10 | 15 | 12 | 14 |
Bud Site | 8 | 8 | 0 | 1 | 2 | 4 | 12 | 9 | 4 | 3 | 10 | 6 | 2 | 2 | 0 | 8 | 2 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 2 |
Cytoplasm | 77 | 126 | 39 | 23 | 45 | 59 | 68 | 86 | 38 | 53 | 57 | 67 | 70 | 61 | 82 | 90 | 75 | 79 | 44 | 64 | 24 | 35 | 40 | 44 |
Endoplasmic Reticulum | 1 | 4 | 2 | 2 | 0 | 2 | 0 | 3 | 1 | 1 | 1 | 4 | 0 | 0 | 0 | 15 | 6 | 8 | 0 | 0 | 2 | 0 | 0 | 0 |
Endosome | 10 | 49 | 0 | 0 | 5 | 5 | 11 | 4 | 6 | 3 | 2 | 6 | 4 | 17 | 23 | 27 | 42 | 44 | 26 | 57 | 13 | 12 | 13 | 12 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 |
Mitochondria | 32 | 16 | 2 | 11 | 2 | 26 | 34 | 42 | 50 | 74 | 111 | 78 | 1 | 1 | 0 | 15 | 3 | 8 | 20 | 46 | 23 | 12 | 15 | 22 |
Nucleus | 0 | 0 | 2 | 1 | 1 | 3 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 3 | 3 | 2 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 12 | 27 | 0 | 1 | 1 | 7 | 10 | 2 | 5 | 4 | 1 | 7 | 2 | 6 | 11 | 9 | 10 | 23 | 14 | 17 | 5 | 7 | 4 | 9 |
SpindlePole | 73 | 115 | 34 | 14 | 55 | 56 | 98 | 119 | 61 | 69 | 47 | 62 | 44 | 64 | 83 | 90 | 99 | 89 | 86 | 134 | 36 | 27 | 58 | 56 |
Vac/Vac Membrane | 0 | 8 | 1 | 0 | 2 | 1 | 6 | 0 | 2 | 3 | 1 | 3 | 0 | 0 | 0 | 8 | 6 | 3 | 1 | 7 | 1 | 1 | 2 | 1 |
Unique Cell Count | 194 | 323 | 90 | 53 | 116 | 146 | 216 | 250 | 151 | 180 | 199 | 197 | 129 | 147 | 188 | 222 | 213 | 202 | 228 | 402 | 137 | 149 | 189 | 219 |
Labelled Cell Count | 241 | 396 | 110 | 72 | 145 | 188 | 280 | 349 | 191 | 260 | 264 | 257 | 152 | 189 | 222 | 285 | 276 | 282 | 228 | 402 | 137 | 149 | 189 | 219 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 4.6 | 5.4 | 4.5 | 5.4 | 4.2 | 4.3 | 4.3 | 3.7 | 3.8 | 3.3 | 4.8 | 5.3 | 5.2 | 5.0 | 6.4 | 6.1 | 5.8 | 4.7 | 5.1 | 5.2 |
Std Deviation (1e-4) | 0.6 | 0.8 | 2.1 | 1.0 | 1.7 | 1.2 | 1.3 | 0.9 | 1.0 | 1.5 | 1.6 | 4.1 | 1.0 | 0.9 | 1.3 | 2.0 | 1.5 | 1.5 | 1.1 | 1.3 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.26 | 0.01 | -0.35 | -0.33 | -0.32 | -0.55 | -0.52 | -0.72 | -0.18 | -0.04 | -0.05 | -0.1 | 0.25 | 0.18 | 0.09 | -0.22 | -0.08 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.5444 | -1.0464 | -0.9002 | -1.2633 | -1.3374 | -0.8629 | -0.9323 | -0.4936 | -0.4676 | -0.8351 | -0.8123 | -0.3997 | -1.4092 | -0.7453 | -0.8649 | -0.742 | -0.7751 | -0.8356 |
Actin | 0.0436 | 0.016 | 0.0094 | 0.0024 | 0.0345 | 0.0062 | 0.0033 | 0.0047 | 0.0055 | 0.0006 | 0.0018 | 0.0056 | 0.0012 | 0.0028 | 0.0017 | 0.0016 | 0.0015 | 0.0024 |
Bud | 0.0011 | 0.0007 | 0.0049 | 0.0007 | 0.0073 | 0.0041 | 0.0005 | 0.0009 | 0.0004 | 0.0001 | 0.0013 | 0.0002 | 0.0013 | 0.0034 | 0.0091 | 0.0001 | 0.0013 | 0.0002 |
Bud Neck | 0.0035 | 0.0022 | 0.0699 | 0.0534 | 0.0006 | 0.0236 | 0.0133 | 0.0071 | 0.1024 | 0.108 | 0.0006 | 0.0128 | 0.0075 | 0.01 | 0.0586 | 0.0076 | 0.0007 | 0.0279 |
Bud Periphery | 0.002 | 0.0013 | 0.003 | 0.0025 | 0.0173 | 0.0022 | 0.0009 | 0.0014 | 0.0007 | 0.0003 | 0.0061 | 0.0004 | 0.0009 | 0.0017 | 0.0058 | 0.0001 | 0.0073 | 0.0003 |
Bud Site | 0.0049 | 0.025 | 0.008 | 0.0039 | 0.0019 | 0.0011 | 0.0151 | 0.0517 | 0.0139 | 0.0012 | 0.0012 | 0.0006 | 0.0048 | 0.0658 | 0.0242 | 0.0007 | 0.0013 | 0.0007 |
Cell Periphery | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0002 | 0.0001 | 0.0003 | 0.0002 |
Cytoplasm | 0.0413 | 0.045 | 0.0177 | 0.0094 | 0.014 | 0.0675 | 0.021 | 0.0313 | 0.0096 | 0.0084 | 0.0065 | 0.0276 | 0.0369 | 0.0756 | 0.0287 | 0.0246 | 0.0674 | 0.0682 |
Cytoplasmic Foci | 0.3158 | 0.27 | 0.4549 | 0.5035 | 0.2538 | 0.2149 | 0.3576 | 0.1771 | 0.3661 | 0.3178 | 0.2741 | 0.306 | 0.5314 | 0.3107 | 0.5201 | 0.3721 | 0.3487 | 0.3957 |
Eisosomes | 0.0008 | 0.0009 | 0.0004 | 0.0002 | 0.0006 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0002 |
Endoplasmic Reticulum | 0.0033 | 0.0018 | 0.001 | 0.0005 | 0.0007 | 0.0014 | 0.0009 | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0013 | 0.0019 | 0.0005 | 0.0025 | 0.0006 | 0.0013 |
Endosome | 0.0559 | 0.0111 | 0.0148 | 0.0289 | 0.0384 | 0.023 | 0.013 | 0.01 | 0.0058 | 0.005 | 0.0176 | 0.0148 | 0.0251 | 0.0219 | 0.0105 | 0.0895 | 0.0172 | 0.0171 |
Golgi | 0.0169 | 0.003 | 0.0093 | 0.0079 | 0.0168 | 0.0036 | 0.003 | 0.0029 | 0.0025 | 0.0027 | 0.0054 | 0.0026 | 0.0032 | 0.0052 | 0.004 | 0.0139 | 0.0062 | 0.0033 |
Lipid Particles | 0.0659 | 0.0246 | 0.0265 | 0.0356 | 0.0866 | 0.028 | 0.0261 | 0.0136 | 0.0138 | 0.0202 | 0.018 | 0.0214 | 0.0544 | 0.0445 | 0.0252 | 0.0277 | 0.0843 | 0.0273 |
Mitochondria | 0.0147 | 0.0022 | 0.0221 | 0.0161 | 0.0349 | 0.003 | 0.0026 | 0.0024 | 0.0033 | 0.0018 | 0.015 | 0.0043 | 0.001 | 0.0043 | 0.0019 | 0.0021 | 0.0252 | 0.0015 |
None | 0.2722 | 0.5246 | 0.1965 | 0.0905 | 0.2384 | 0.5422 | 0.3437 | 0.4748 | 0.2239 | 0.2455 | 0.2745 | 0.4407 | 0.2057 | 0.2746 | 0.1376 | 0.2371 | 0.1241 | 0.3464 |
Nuclear Periphery | 0.0182 | 0.0012 | 0.0009 | 0.0004 | 0.0006 | 0.0042 | 0.0011 | 0.0011 | 0.0005 | 0.0005 | 0.0003 | 0.0008 | 0.0007 | 0.0013 | 0.0012 | 0.0007 | 0.0004 | 0.0008 |
Nucleolus | 0.002 | 0.0014 | 0.0036 | 0.003 | 0.0011 | 0.0011 | 0.0042 | 0.0082 | 0.0072 | 0.002 | 0.0012 | 0.0021 | 0.0052 | 0.0026 | 0.0028 | 0.0002 | 0.0012 | 0.0006 |
Nucleus | 0.0051 | 0.0012 | 0.0025 | 0.0011 | 0.0008 | 0.0018 | 0.0043 | 0.0097 | 0.0029 | 0.0008 | 0.0008 | 0.002 | 0.0023 | 0.0039 | 0.0061 | 0.0003 | 0.0006 | 0.0011 |
Peroxisomes | 0.1006 | 0.0477 | 0.0959 | 0.1871 | 0.2409 | 0.0448 | 0.0954 | 0.0776 | 0.0979 | 0.2309 | 0.3453 | 0.0803 | 0.0686 | 0.111 | 0.0956 | 0.185 | 0.3028 | 0.0582 |
Punctate Nuclear | 0.026 | 0.0182 | 0.0535 | 0.0494 | 0.0033 | 0.0245 | 0.0915 | 0.1219 | 0.1421 | 0.0521 | 0.0273 | 0.0763 | 0.0466 | 0.0538 | 0.0645 | 0.0325 | 0.0055 | 0.0454 |
Vacuole | 0.0032 | 0.0014 | 0.0035 | 0.0026 | 0.0056 | 0.0014 | 0.0016 | 0.0019 | 0.0007 | 0.0015 | 0.0019 | 0.0007 | 0.0013 | 0.0033 | 0.0011 | 0.0009 | 0.0025 | 0.0012 |
Vacuole Periphery | 0.0026 | 0.0003 | 0.0014 | 0.0006 | 0.0017 | 0.0009 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0008 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.8386 | 9.1756 | 3.7826 | 4.9437 | 7.763 | 8.5488 | 10.9525 | 8.1749 | 7.1726 | 11.6112 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
128 | 1555 | 1610 | 1598 | 1668 | 2233 | 1284 | 88 | 1796 | 3788 | 2894 | 1686 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 586.34 | 750.48 | 866.43 | 815.93 | 652.45 | 691.27 | 840.97 | 822.72 | 647.74 | 715.58 | 855.13 | 816.28 |
Standard Deviation | 65.53 | 89.93 | 103.58 | 101.54 | 68.32 | 90.09 | 105.79 | 109.28 | 70.22 | 94.62 | 105.33 | 101.97 |
Intensity Change Log 2 | — | 0.356076 | 0.563346 | 0.476708 | — | 0.083382 | 0.366187 | 0.334534 | — | 0.218889 | 0.462869 | 0.403576 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000222 | 0.000720 | 0.003126 | 0.007953 | 0.000553 | 0.001494 | 0.001851 | 0.008146 | 0.000530 | 0.001176 | 0.002560 | 0.007963 |
Bud Neck | 0.061439 | 0.059063 | 0.047190 | 0.054902 | 0.050001 | 0.063078 | 0.035054 | 0.050653 | 0.050816 | 0.061430 | 0.041805 | 0.054680 |
Bud Site | 0.010121 | 0.029997 | 0.080422 | 0.182536* | 0.020367 | 0.033391 | 0.075048 | 0.258229* | 0.019637 | 0.031998 | 0.078037 | 0.186487* |
Cell Periphery | 0.000588 | 0.000088 | 0.000269 | 0.000319 | 0.000124 | 0.000138 | 0.000263 | 0.000404 | 0.000157 | 0.000117 | 0.000267 | 0.000323 |
Cytoplasm | 0.145120* | 0.165158* | 0.206343* | 0.224116* | 0.165169* | 0.136746* | 0.263839* | 0.138181* | 0.163740* | 0.148410* | 0.231853* | 0.219630* |
Cytoplasmic Foci | 0.488603* | 0.285572* | 0.071393 | 0.114121 | 0.443158* | 0.366860* | 0.061891 | 0.128432 | 0.446397* | 0.333491* | 0.067177 | 0.114868 |
Eisosomes | 0.000913 | 0.000098 | 0.000108 | 0.000110 | 0.000175 | 0.000159 | 0.000108 | 0.000151 | 0.000228 | 0.000134 | 0.000108 | 0.000112 |
Endoplasmic Reticulum | 0.000593 | 0.000606 | 0.005387 | 0.001743 | 0.000402 | 0.000343 | 0.004054 | 0.001433 | 0.000415 | 0.000451 | 0.004795 | 0.001727 |
Endosome | 0.025359 | 0.057092 | 0.015070 | 0.020346 | 0.039571 | 0.066843 | 0.015632 | 0.045110 | 0.038558 | 0.062840 | 0.015319 | 0.021639 |
Golgi | 0.018690 | 0.013654 | 0.001244 | 0.002569 | 0.013453 | 0.034915 | 0.001167 | 0.010492 | 0.013826 | 0.026187 | 0.001210 | 0.002983 |
Lipid Particles | 0.040080 | 0.008704 | 0.007045 | 0.003078 | 0.018820 | 0.014394 | 0.004176 | 0.004137 | 0.020335 | 0.012058 | 0.005772 | 0.003133 |
Mitochondria | 0.017228 | 0.023153 | 0.002435 | 0.002594 | 0.012422 | 0.031389 | 0.002028 | 0.002315 | 0.012765 | 0.028008 | 0.002255 | 0.002579 |
Mitotic Spindle | 0.002393 | 0.088522 | 0.117897* | 0.092264 | 0.016260 | 0.046101 | 0.061741 | 0.029992 | 0.015272 | 0.063515 | 0.092982 | 0.089014 |
None | 0.004918 | 0.002239 | 0.005287 | 0.004608 | 0.002940 | 0.002136 | 0.010106 | 0.000685 | 0.003081 | 0.002179 | 0.007425 | 0.004404 |
Nuclear Periphery | 0.000290 | 0.000726 | 0.002354 | 0.000746 | 0.000290 | 0.000204 | 0.001712 | 0.000671 | 0.000290 | 0.000418 | 0.002069 | 0.000743 |
Nuclear Periphery Foci | 0.000471 | 0.000880 | 0.006225 | 0.002095 | 0.000798 | 0.000457 | 0.003835 | 0.002943 | 0.000775 | 0.000631 | 0.005165 | 0.002139 |
Nucleolus | 0.007640 | 0.007255 | 0.008406 | 0.002180 | 0.004387 | 0.002294 | 0.007122 | 0.000753 | 0.004619 | 0.004331 | 0.007837 | 0.002106 |
Nucleus | 0.060906 | 0.064548 | 0.300527* | 0.101836 | 0.044718 | 0.015309 | 0.333558* | 0.029424 | 0.045871 | 0.035522 | 0.315182* | 0.098056 |
Peroxisomes | 0.108795* | 0.154527* | 0.068199 | 0.115522* | 0.149192* | 0.155510* | 0.054407 | 0.170811* | 0.146313* | 0.155106* | 0.062080 | 0.118408* |
Vacuole | 0.004887 | 0.035642 | 0.050542 | 0.065558 | 0.015716 | 0.026235 | 0.061678 | 0.108256 | 0.014944 | 0.030096 | 0.055483 | 0.067787 |
Vacuole Periphery | 0.000742 | 0.001756 | 0.000529 | 0.000803 | 0.001484 | 0.002004 | 0.000730 | 0.008781 | 0.001431 | 0.001902 | 0.000618 | 0.001220 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -8.02 | -11.31 | -6.89 | -6.22 | -2.72 | -4.47 | -10.60 | -2.93 | -2.43 | -1.88 | -5.04 | -13.89 | -6.85 | -5.88 | -3.43 |
Bud Neck | 0.30 | 0.74 | 0.68 | 1.33 | -0.22 | -3.81 | 4.23 | 2.70 | 4.66 | 0.77 | -3.26 | 2.06 | -0.07 | 3.36 | -2.28 |
Bud Site | -6.29 | -13.91 | -20.86 | -18.35 | -9.68 | -5.95 | -10.18 | -5.35 | -4.78 | -3.61 | -6.44 | -15.61 | -20.99 | -18.84 | -11.26 |
Cell Periphery | 3.87 | 2.59 | 2.43 | -8.68 | -0.76 | -1.20 | -9.32 | -2.76 | -2.47 | -0.39 | 2.86 | -6.97 | -5.05 | -7.38 | -0.64 |
Cytoplasm | -1.20 | -1.00 | -4.29 | -7.02 | -7.79 | 3.16 | -5.90 | 0.53 | -0.40 | 2.67 | 1.68 | -3.50 | -7.44 | -10.11 | -4.83 |
Cytoplasmic Foci | 7.90 | 16.82 | 15.67 | 28.21 | -5.59 | 10.15 | 55.52 | 18.74 | 14.57 | -2.72 | 16.40 | 60.60 | 50.63 | 44.81 | -7.16 |
Eisosomes | 2.38 | 2.23 | 2.29 | -5.15 | 2.66 | 0.97 | 0.56 | -0.23 | -0.74 | -0.47 | 3.38 | 2.57 | 3.47 | 0.06 | 3.65 |
Endoplasmic Reticulum | -0.45 | -16.99 | -7.89 | -12.73 | 12.95 | 0.46 | -19.46 | -4.08 | -4.16 | 5.06 | -0.88 | -27.27 | -14.32 | -14.71 | 15.76 |
Endosome | -5.69 | 1.42 | 1.67 | 14.34 | 0.88 | -8.66 | 9.35 | 1.61 | 4.66 | -0.95 | -9.06 | 10.10 | 9.72 | 22.08 | 0.62 |
Golgi | 1.41 | 4.03 | 3.98 | 14.07 | -1.64 | -12.22 | 13.76 | 3.36 | 10.55 | -1.44 | -9.19 | 14.21 | 13.44 | 24.25 | -2.70 |
Lipid Particles | 3.13 | 3.18 | 3.65 | 6.30 | 7.57 | 2.78 | 9.57 | 7.49 | 5.26 | 0.95 | 5.41 | 9.17 | 11.74 | 10.34 | 7.93 |
Mitochondria | -0.91 | 3.36 | 3.52 | 10.69 | 1.98 | -9.58 | 11.45 | 11.09 | 16.50 | 1.78 | -9.36 | 11.53 | 11.32 | 19.19 | 1.25 |
Mitotic Spindle | -17.22* | -22.25* | -17.96 | -1.45 | 5.11 | -7.98 | -11.69 | -2.01 | -0.37 | 2.06 | -14.75 | -21.90 | -14.97 | -6.10 | 2.79 |
None | 2.05 | -0.51 | 0.63 | -3.27 | 2.53 | 2.22 | -5.00 | 5.25 | 2.12 | 8.34 | 2.81 | -5.75 | -0.86 | -3.30 | 4.17 |
Nuclear Periphery | -2.72 | -15.96 | -9.02 | -6.29 | 9.88 | 2.14 | -18.09 | -3.74 | -4.10 | 5.03 | -2.30 | -25.61 | -14.38 | -11.85 | 10.33 |
Nuclear Periphery Foci | -4.70 | -11.56 | -8.02 | -5.31 | 7.42 | 3.54 | -8.53 | -4.36 | -5.36 | 5.64 | 0.92 | -13.72 | -6.75 | -7.25 | 8.94 |
Nucleolus | -0.37 | -2.71 | 0.98 | 4.29 | 9.85 | 4.89 | -4.89 | 7.55 | 5.57 | 9.34 | 0.80 | -8.79 | 1.84 | 1.30 | 11.07 |
Nucleus | -0.35 | -14.66 | -5.44 | -10.75 | 15.17 | 10.23 | -28.80 | -0.44 | -3.18 | 19.48 | 2.71 | -39.08 | -15.36 | -17.67 | 20.68 |
Peroxisomes | -2.33 | 4.03 | 2.51 | 11.13 | -4.43 | -1.42 | 17.92 | 1.80 | 2.23 | -2.97 | -1.34 | 18.98 | 11.60 | 15.12 | -6.15 |
Vacuole | -15.10 | -21.22 | -26.39 | -18.81 | -10.42 | -6.40 | -18.94 | -8.19 | -7.87 | -5.60 | -11.20 | -25.82 | -25.49 | -22.10 | -10.05 |
Vacuole Periphery | -3.70 | 1.29 | 1.13 | 5.35 | 0.13 | -1.47 | 2.32 | -0.93 | -0.70 | -1.28 | -1.34 | 2.47 | 1.85 | 5.35 | -0.69 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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