Standard name
Human Ortholog
Description Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.07 0.11 0.05 0 0 0 0 0 0 0 0.08 0.09 0 0 0 0 0.06 0.06 0.05 0.11 0.12 0.14
Bud 0.08 0.06 0.06 0.11 0.09 0.05 0.08 0.14 0.07 0.16 0.1 0.05 0.05 0.06 0 0 0.05 0 0 0 0 0.09 0.06 0.07
Bud Neck 0 0 0.21 0.13 0.14 0.1 0.09 0.17 0.08 0.09 0.05 0 0.1 0.11 0.07 0 0 0 0.05 0.05 0.08 0.1 0.07 0.07
Bud Site 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 0.39 0.43 0.43 0.39 0.4 0.31 0.34 0.25 0.29 0.29 0.34 0.54 0.41 0.44 0.41 0.35 0.39 0.19 0.16 0.18 0.24 0.21 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0.05 0.15 0 0 0 0 0.05 0 0 0 0 0 0 0.12 0.12 0.12 0.2 0.22 0.12 0.14 0.1 0.08 0.07 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.05 0 0.21 0 0.18 0.16 0.17 0.33 0.41 0.56 0.4 0 0 0 0.07 0 0 0.09 0.12 0.17 0.08 0.08 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0.08 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0.06 0 0.05 0.11 0.07 0 0 0.05 0 0
SpindlePole 0.38 0.36 0.38 0.26 0.47 0.38 0.45 0.48 0.4 0.38 0.24 0.31 0.34 0.44 0.44 0.41 0.46 0.44 0.38 0.33 0.26 0.18 0.31 0.26
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 6 9 6 6 6 3 4 6 1 0 0 2 10 13 3 6 8 2 13 24 6 16 22 30
Bud 16 20 5 6 10 8 17 35 10 28 20 10 6 9 7 6 10 5 5 13 5 14 11 16
Bud Neck 5 12 19 7 16 14 19 42 12 17 10 7 13 16 13 5 9 6 12 21 10 15 12 14
Bud Site 8 8 0 1 2 4 12 9 4 3 10 6 2 2 0 8 2 4 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 1 0 2 0 0 0 1 2 2 0 1 0 0 0 2
Cytoplasm 77 126 39 23 45 59 68 86 38 53 57 67 70 61 82 90 75 79 44 64 24 35 40 44
Endoplasmic Reticulum 1 4 2 2 0 2 0 3 1 1 1 4 0 0 0 15 6 8 0 0 2 0 0 0
Endosome 10 49 0 0 5 5 11 4 6 3 2 6 4 17 23 27 42 44 26 57 13 12 13 12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 0 2 0 0 0 0
Mitochondria 32 16 2 11 2 26 34 42 50 74 111 78 1 1 0 15 3 8 20 46 23 12 15 22
Nucleus 0 0 2 1 1 3 0 0 0 1 2 1 0 0 0 3 3 2 0 1 0 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 1 1 0 3 2 1 0 0 0 1 1 3 0 1 0 0 0 0
Peroxisomes 12 27 0 1 1 7 10 2 5 4 1 7 2 6 11 9 10 23 14 17 5 7 4 9
SpindlePole 73 115 34 14 55 56 98 119 61 69 47 62 44 64 83 90 99 89 86 134 36 27 58 56
Vac/Vac Membrane 0 8 1 0 2 1 6 0 2 3 1 3 0 0 0 8 6 3 1 7 1 1 2 1
Unique Cell Count 194 323 90 53 116 146 216 250 151 180 199 197 129 147 188 222 213 202 228 402 137 149 189 219
Labelled Cell Count 241 396 110 72 145 188 280 349 191 260 264 257 152 189 222 285 276 282 228 402 137 149 189 219


Ambiguous, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.6 5.4 4.5 5.4 4.2 4.3 4.3 3.7 3.8 3.3 4.8 5.3 5.2 5.0 6.4 6.1 5.8 4.7 5.1 5.2
Std Deviation (1e-4) 0.6 0.8 2.1 1.0 1.7 1.2 1.3 0.9 1.0 1.5 1.6 4.1 1.0 0.9 1.3 2.0 1.5 1.5 1.1 1.3 1.2
Intensity Change (Log2) – – – -0.26 0.01 -0.35 -0.33 -0.32 -0.55 -0.52 -0.72 -0.18 -0.04 -0.05 -0.1 0.25 0.18 0.09 -0.22 -0.08 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5444 -1.0464 -0.9002 -1.2633 -1.3374 -0.8629 -0.9323 -0.4936 -0.4676 -0.8351 -0.8123 -0.3997 -1.4092 -0.7453 -0.8649 -0.742 -0.7751 -0.8356
Actin 0.0436 0.016 0.0094 0.0024 0.0345 0.0062 0.0033 0.0047 0.0055 0.0006 0.0018 0.0056 0.0012 0.0028 0.0017 0.0016 0.0015 0.0024
Bud 0.0011 0.0007 0.0049 0.0007 0.0073 0.0041 0.0005 0.0009 0.0004 0.0001 0.0013 0.0002 0.0013 0.0034 0.0091 0.0001 0.0013 0.0002
Bud Neck 0.0035 0.0022 0.0699 0.0534 0.0006 0.0236 0.0133 0.0071 0.1024 0.108 0.0006 0.0128 0.0075 0.01 0.0586 0.0076 0.0007 0.0279
Bud Periphery 0.002 0.0013 0.003 0.0025 0.0173 0.0022 0.0009 0.0014 0.0007 0.0003 0.0061 0.0004 0.0009 0.0017 0.0058 0.0001 0.0073 0.0003
Bud Site 0.0049 0.025 0.008 0.0039 0.0019 0.0011 0.0151 0.0517 0.0139 0.0012 0.0012 0.0006 0.0048 0.0658 0.0242 0.0007 0.0013 0.0007
Cell Periphery 0.0005 0.0004 0.0005 0.0002 0.0003 0.0002 0.0003 0.0004 0.0002 0.0001 0.0001 0.0001 0.0002 0.0008 0.0002 0.0001 0.0003 0.0002
Cytoplasm 0.0413 0.045 0.0177 0.0094 0.014 0.0675 0.021 0.0313 0.0096 0.0084 0.0065 0.0276 0.0369 0.0756 0.0287 0.0246 0.0674 0.0682
Cytoplasmic Foci 0.3158 0.27 0.4549 0.5035 0.2538 0.2149 0.3576 0.1771 0.3661 0.3178 0.2741 0.306 0.5314 0.3107 0.5201 0.3721 0.3487 0.3957
Eisosomes 0.0008 0.0009 0.0004 0.0002 0.0006 0.0003 0.0004 0.0003 0.0003 0.0001 0.0001 0.0002 0.0004 0.0005 0.0005 0.0003 0.0002 0.0002
Endoplasmic Reticulum 0.0033 0.0018 0.001 0.0005 0.0007 0.0014 0.0009 0.0007 0.0002 0.0003 0.0003 0.0004 0.0013 0.0019 0.0005 0.0025 0.0006 0.0013
Endosome 0.0559 0.0111 0.0148 0.0289 0.0384 0.023 0.013 0.01 0.0058 0.005 0.0176 0.0148 0.0251 0.0219 0.0105 0.0895 0.0172 0.0171
Golgi 0.0169 0.003 0.0093 0.0079 0.0168 0.0036 0.003 0.0029 0.0025 0.0027 0.0054 0.0026 0.0032 0.0052 0.004 0.0139 0.0062 0.0033
Lipid Particles 0.0659 0.0246 0.0265 0.0356 0.0866 0.028 0.0261 0.0136 0.0138 0.0202 0.018 0.0214 0.0544 0.0445 0.0252 0.0277 0.0843 0.0273
Mitochondria 0.0147 0.0022 0.0221 0.0161 0.0349 0.003 0.0026 0.0024 0.0033 0.0018 0.015 0.0043 0.001 0.0043 0.0019 0.0021 0.0252 0.0015
None 0.2722 0.5246 0.1965 0.0905 0.2384 0.5422 0.3437 0.4748 0.2239 0.2455 0.2745 0.4407 0.2057 0.2746 0.1376 0.2371 0.1241 0.3464
Nuclear Periphery 0.0182 0.0012 0.0009 0.0004 0.0006 0.0042 0.0011 0.0011 0.0005 0.0005 0.0003 0.0008 0.0007 0.0013 0.0012 0.0007 0.0004 0.0008
Nucleolus 0.002 0.0014 0.0036 0.003 0.0011 0.0011 0.0042 0.0082 0.0072 0.002 0.0012 0.0021 0.0052 0.0026 0.0028 0.0002 0.0012 0.0006
Nucleus 0.0051 0.0012 0.0025 0.0011 0.0008 0.0018 0.0043 0.0097 0.0029 0.0008 0.0008 0.002 0.0023 0.0039 0.0061 0.0003 0.0006 0.0011
Peroxisomes 0.1006 0.0477 0.0959 0.1871 0.2409 0.0448 0.0954 0.0776 0.0979 0.2309 0.3453 0.0803 0.0686 0.111 0.0956 0.185 0.3028 0.0582
Punctate Nuclear 0.026 0.0182 0.0535 0.0494 0.0033 0.0245 0.0915 0.1219 0.1421 0.0521 0.0273 0.0763 0.0466 0.0538 0.0645 0.0325 0.0055 0.0454
Vacuole 0.0032 0.0014 0.0035 0.0026 0.0056 0.0014 0.0016 0.0019 0.0007 0.0015 0.0019 0.0007 0.0013 0.0033 0.0011 0.0009 0.0025 0.0012
Vacuole Periphery 0.0026 0.0003 0.0014 0.0006 0.0017 0.0009 0.0002 0.0002 0.0001 0.0003 0.0005 0.0002 0.0002 0.0004 0.0002 0.0003 0.0008 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.8386 9.1756 3.7826 4.9437 7.763 8.5488 10.9525 8.1749 7.1726 11.6112
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
128 1555 1610 1598 1668 2233 1284 88 1796 3788 2894 1686

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 586.34 750.48 866.43 815.93 652.45 691.27 840.97 822.72 647.74 715.58 855.13 816.28
Standard Deviation 65.53 89.93 103.58 101.54 68.32 90.09 105.79 109.28 70.22 94.62 105.33 101.97
Intensity Change Log 2 — 0.356076 0.563346 0.476708 — 0.083382 0.366187 0.334534 — 0.218889 0.462869 0.403576

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000222 0.000720 0.003126 0.007953 0.000553 0.001494 0.001851 0.008146 0.000530 0.001176 0.002560 0.007963
Bud Neck 0.061439 0.059063 0.047190 0.054902 0.050001 0.063078 0.035054 0.050653 0.050816 0.061430 0.041805 0.054680
Bud Site 0.010121 0.029997 0.080422 0.182536* 0.020367 0.033391 0.075048 0.258229* 0.019637 0.031998 0.078037 0.186487*
Cell Periphery 0.000588 0.000088 0.000269 0.000319 0.000124 0.000138 0.000263 0.000404 0.000157 0.000117 0.000267 0.000323
Cytoplasm 0.145120* 0.165158* 0.206343* 0.224116* 0.165169* 0.136746* 0.263839* 0.138181* 0.163740* 0.148410* 0.231853* 0.219630*
Cytoplasmic Foci 0.488603* 0.285572* 0.071393 0.114121 0.443158* 0.366860* 0.061891 0.128432 0.446397* 0.333491* 0.067177 0.114868
Eisosomes 0.000913 0.000098 0.000108 0.000110 0.000175 0.000159 0.000108 0.000151 0.000228 0.000134 0.000108 0.000112
Endoplasmic Reticulum 0.000593 0.000606 0.005387 0.001743 0.000402 0.000343 0.004054 0.001433 0.000415 0.000451 0.004795 0.001727
Endosome 0.025359 0.057092 0.015070 0.020346 0.039571 0.066843 0.015632 0.045110 0.038558 0.062840 0.015319 0.021639
Golgi 0.018690 0.013654 0.001244 0.002569 0.013453 0.034915 0.001167 0.010492 0.013826 0.026187 0.001210 0.002983
Lipid Particles 0.040080 0.008704 0.007045 0.003078 0.018820 0.014394 0.004176 0.004137 0.020335 0.012058 0.005772 0.003133
Mitochondria 0.017228 0.023153 0.002435 0.002594 0.012422 0.031389 0.002028 0.002315 0.012765 0.028008 0.002255 0.002579
Mitotic Spindle 0.002393 0.088522 0.117897* 0.092264 0.016260 0.046101 0.061741 0.029992 0.015272 0.063515 0.092982 0.089014
None 0.004918 0.002239 0.005287 0.004608 0.002940 0.002136 0.010106 0.000685 0.003081 0.002179 0.007425 0.004404
Nuclear Periphery 0.000290 0.000726 0.002354 0.000746 0.000290 0.000204 0.001712 0.000671 0.000290 0.000418 0.002069 0.000743
Nuclear Periphery Foci 0.000471 0.000880 0.006225 0.002095 0.000798 0.000457 0.003835 0.002943 0.000775 0.000631 0.005165 0.002139
Nucleolus 0.007640 0.007255 0.008406 0.002180 0.004387 0.002294 0.007122 0.000753 0.004619 0.004331 0.007837 0.002106
Nucleus 0.060906 0.064548 0.300527* 0.101836 0.044718 0.015309 0.333558* 0.029424 0.045871 0.035522 0.315182* 0.098056
Peroxisomes 0.108795* 0.154527* 0.068199 0.115522* 0.149192* 0.155510* 0.054407 0.170811* 0.146313* 0.155106* 0.062080 0.118408*
Vacuole 0.004887 0.035642 0.050542 0.065558 0.015716 0.026235 0.061678 0.108256 0.014944 0.030096 0.055483 0.067787
Vacuole Periphery 0.000742 0.001756 0.000529 0.000803 0.001484 0.002004 0.000730 0.008781 0.001431 0.001902 0.000618 0.001220

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.02 -11.31 -6.89 -6.22 -2.72 -4.47 -10.60 -2.93 -2.43 -1.88 -5.04 -13.89 -6.85 -5.88 -3.43
Bud Neck 0.30 0.74 0.68 1.33 -0.22 -3.81 4.23 2.70 4.66 0.77 -3.26 2.06 -0.07 3.36 -2.28
Bud Site -6.29 -13.91 -20.86 -18.35 -9.68 -5.95 -10.18 -5.35 -4.78 -3.61 -6.44 -15.61 -20.99 -18.84 -11.26
Cell Periphery 3.87 2.59 2.43 -8.68 -0.76 -1.20 -9.32 -2.76 -2.47 -0.39 2.86 -6.97 -5.05 -7.38 -0.64
Cytoplasm -1.20 -1.00 -4.29 -7.02 -7.79 3.16 -5.90 0.53 -0.40 2.67 1.68 -3.50 -7.44 -10.11 -4.83
Cytoplasmic Foci 7.90 16.82 15.67 28.21 -5.59 10.15 55.52 18.74 14.57 -2.72 16.40 60.60 50.63 44.81 -7.16
Eisosomes 2.38 2.23 2.29 -5.15 2.66 0.97 0.56 -0.23 -0.74 -0.47 3.38 2.57 3.47 0.06 3.65
Endoplasmic Reticulum -0.45 -16.99 -7.89 -12.73 12.95 0.46 -19.46 -4.08 -4.16 5.06 -0.88 -27.27 -14.32 -14.71 15.76
Endosome -5.69 1.42 1.67 14.34 0.88 -8.66 9.35 1.61 4.66 -0.95 -9.06 10.10 9.72 22.08 0.62
Golgi 1.41 4.03 3.98 14.07 -1.64 -12.22 13.76 3.36 10.55 -1.44 -9.19 14.21 13.44 24.25 -2.70
Lipid Particles 3.13 3.18 3.65 6.30 7.57 2.78 9.57 7.49 5.26 0.95 5.41 9.17 11.74 10.34 7.93
Mitochondria -0.91 3.36 3.52 10.69 1.98 -9.58 11.45 11.09 16.50 1.78 -9.36 11.53 11.32 19.19 1.25
Mitotic Spindle -17.22* -22.25* -17.96 -1.45 5.11 -7.98 -11.69 -2.01 -0.37 2.06 -14.75 -21.90 -14.97 -6.10 2.79
None 2.05 -0.51 0.63 -3.27 2.53 2.22 -5.00 5.25 2.12 8.34 2.81 -5.75 -0.86 -3.30 4.17
Nuclear Periphery -2.72 -15.96 -9.02 -6.29 9.88 2.14 -18.09 -3.74 -4.10 5.03 -2.30 -25.61 -14.38 -11.85 10.33
Nuclear Periphery Foci -4.70 -11.56 -8.02 -5.31 7.42 3.54 -8.53 -4.36 -5.36 5.64 0.92 -13.72 -6.75 -7.25 8.94
Nucleolus -0.37 -2.71 0.98 4.29 9.85 4.89 -4.89 7.55 5.57 9.34 0.80 -8.79 1.84 1.30 11.07
Nucleus -0.35 -14.66 -5.44 -10.75 15.17 10.23 -28.80 -0.44 -3.18 19.48 2.71 -39.08 -15.36 -17.67 20.68
Peroxisomes -2.33 4.03 2.51 11.13 -4.43 -1.42 17.92 1.80 2.23 -2.97 -1.34 18.98 11.60 15.12 -6.15
Vacuole -15.10 -21.22 -26.39 -18.81 -10.42 -6.40 -18.94 -8.19 -7.87 -5.60 -11.20 -25.82 -25.49 -22.10 -10.05
Vacuole Periphery -3.70 1.29 1.13 5.35 0.13 -1.47 2.32 -0.93 -0.70 -1.28 -1.34 2.47 1.85 5.35 -0.69
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Kar9

Kar9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kar9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available