ORF
Human Ortholog
Description Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.88 0.87 0.83 0.94 0.88 0.88 0.82 0.76 – 0.68 0.63 0.62 – – 0.96 – – 0.94 0.26 0.28 0.15 0.2 0.24 0.23
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0.06 0.23 0.11 0.27 0.14 0.12 0.14
Endosome 0 0 0.1 0 0.06 0 0 0 – 0 0 0 – – 0 – – 0 0.05 0.08 0.12 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.1 0.07 0.29 0.39 0.38 – 0.59 0.61 0.55 – – 0 – – 0 0.15 0.17 0.17 0.18 0.13 0.16
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.07 0.09 0 0.07 0 0.06 0 – 0 0 0 – – 0 – – 0 0.25 0.3 0.19 0.36 0.41 0.38
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 – 0 0 0 – – 1 – – 0 1 3 9 1 3 0
Bud 0 4 2 2 0 5 10 10 – 8 11 11 – – 0 – – 0 0 1 3 1 1 2
Bud Neck 1 0 0 0 0 0 0 1 – 0 0 1 – – 0 – – 0 3 4 3 1 1 0
Bud Site 0 1 0 0 0 0 0 1 – 0 0 0 – – 0 – – 0 – – – – – –
Cell Periphery 1 0 0 0 2 0 2 4 – 2 4 3 – – 0 – – 0 0 0 0 0 0 0
Cytoplasm 207 163 229 202 215 287 416 389 – 263 288 276 – – 145 – – 189 69 64 45 25 54 45
Endoplasmic Reticulum 9 5 3 0 2 2 6 3 – 2 6 9 – – 4 – – 12 60 25 81 17 26 29
Endosome 6 1 29 6 14 4 4 8 – 2 1 2 – – 0 – – 5 14 17 35 3 9 8
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 – – 0 – – 1 3 0 3 1 1 0
Mitochondria 3 11 11 21 17 95 195 196 – 228 277 245 – – 1 – – 8 38 38 52 24 29 32
Nucleus 0 0 0 0 0 0 0 4 – 1 1 3 – – 0 – – 2 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 – 1 0 1 – – 0 – – 0 1 0 2 1 0 0
Nucleolus 0 0 1 1 0 1 0 3 – 1 2 0 – – 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 2 0 1 0 0 0 – 0 0 0 – – 0 – – 0 0 0 0 0 0 1
SpindlePole 0 0 2 0 1 1 3 0 – 2 1 3 – – 0 – – 0 0 0 1 0 1 1
Vac/Vac Membrane 14 14 25 4 16 6 31 10 – 8 18 13 – – 1 – – 8 65 68 57 47 91 75
Unique Cell Count 235 187 277 216 245 327 505 514 387 455 443 151 202 262 229 301 131 226 201
Labelled Cell Count 242 201 304 236 268 401 667 629 518 609 567 152 225 262 229 301 131 226 201


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.8 4.6 4.4 4.9 3.6 3.6 3.8 – 3.3 3.3 3.3 – – 5.9 4.3 4.5 5.5 5.5 6.3 6.7
Std Deviation (1e-4) 0.5 1.1 1.7 1.0 2.6 0.8 1.1 1.2 – 1.0 0.8 0.9 – – 1.0 0.9 0.8 1.3 1.5 1.9 2.1
Intensity Change (Log2) – – – -0.08 0.08 -0.35 -0.37 -0.29 – -0.5 -0.46 -0.49 – – 0.35 -0.1 -0.05 0.24 0.26 0.46 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – -2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1211 1.7363 1.5799 1.7792 1.5875 1.4137 2.0638 2.887 2.8888 2.9487 2.266 2.8199 2.001 2.9573 2.595 2.6902 3.3795 2.76
Actin 0.0452 0.0001 0.0107 0.01 0.0126 0.0019 0.0079 0.0003 0.0192 0.0024 0.0001 0.0007 0.0159 0.0001 0.0004 0.0013 0 0.002
Bud 0.001 0.0001 0.001 0.0044 0.0051 0.0004 0.0005 0.0002 0.0007 0.0013 0.0001 0.0005 0.0004 0.0001 0.0003 0.0002 0.0002 0.0002
Bud Neck 0.0007 0.0002 0.0003 0.0114 0.0007 0.0011 0.0009 0.0002 0.0015 0.0005 0.0004 0.0012 0.0002 0.0001 0.0002 0.0013 0.0003 0.0012
Bud Periphery 0.0013 0.0002 0.0014 0.0034 0.007 0.0004 0.0011 0.0003 0.0012 0.0018 0.0002 0.001 0.0005 0.0001 0.0003 0.0002 0.0002 0.0002
Bud Site 0.0023 0.0019 0.0021 0.0059 0.0186 0.0014 0.002 0.0013 0.0027 0.0015 0.0002 0.0003 0.001 0.0008 0.0023 0.0004 0.0002 0.0003
Cell Periphery 0.0004 0.0005 0.0005 0.0003 0.0012 0.0002 0.0007 0.001 0.0005 0.0008 0.0007 0.0007 0.0008 0.0007 0.0007 0.0006 0.0003 0.0005
Cytoplasm 0.3029 0.4674 0.3533 0.4677 0.4345 0.5089 0.3783 0.3708 0.3494 0.3169 0.524 0.4554 0.3 0.5507 0.419 0.497 0.6697 0.4679
Cytoplasmic Foci 0.0501 0.0345 0.0501 0.0432 0.0259 0.0441 0.0694 0.0389 0.061 0.0467 0.0218 0.0373 0.0755 0.0251 0.0489 0.0533 0.0205 0.0309
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0002 0 0 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0729 0.0685 0.0415 0.077 0.0352 0.0658 0.0558 0.0863 0.0405 0.0849 0.0386 0.077 0.0153 0.0825 0.0319 0.0813 0.0716 0.0798
Endosome 0.1217 0.0917 0.1735 0.0995 0.1627 0.1361 0.1422 0.1252 0.1409 0.1874 0.1434 0.1254 0.0402 0.0858 0.1039 0.2197 0.0799 0.1044
Golgi 0.0104 0.0015 0.0088 0.0271 0.0146 0.0032 0.0088 0.002 0.0063 0.0049 0.0014 0.0053 0.0124 0.0019 0.0065 0.0035 0.0007 0.008
Lipid Particles 0.0126 0.0074 0.016 0.0057 0.0097 0.0101 0.0239 0.0147 0.0122 0.0044 0.0112 0.0083 0.0349 0.0093 0.0116 0.013 0.0044 0.0108
Mitochondria 0.0068 0.0013 0.009 0.0087 0.0155 0.0039 0.0108 0.001 0.0025 0.0039 0.0009 0.0116 0.0022 0.0011 0.0015 0.0005 0.0011 0.0025
None 0.1988 0.2357 0.2144 0.1412 0.1766 0.148 0.234 0.2854 0.3066 0.2468 0.1754 0.2005 0.4052 0.1894 0.2976 0.0494 0.0375 0.2016
Nuclear Periphery 0.0706 0.015 0.0184 0.0231 0.0148 0.0105 0.0168 0.014 0.0088 0.0102 0.0059 0.0097 0.0035 0.0043 0.0088 0.0078 0.0355 0.0092
Nucleolus 0.0042 0.0004 0.0008 0.0006 0.0008 0.0003 0.0006 0.0004 0.0008 0.0002 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0002
Nucleus 0.0439 0.0044 0.0053 0.0124 0.0073 0.0044 0.0033 0.0055 0.0035 0.0021 0.0025 0.0032 0.0006 0.0017 0.0022 0.0019 0.0059 0.0026
Peroxisomes 0.0044 0.0004 0.0105 0.0036 0.0022 0.0014 0.0035 0.0002 0.0017 0.0269 0.0001 0.0006 0.0689 0.0001 0.008 0.001 0.0001 0.0013
Punctate Nuclear 0.0213 0.0012 0.0037 0.0036 0.0092 0.0046 0.0016 0.0028 0.0034 0.0008 0.0005 0.001 0.0133 0.0005 0.0011 0.0009 0.0004 0.0006
Vacuole 0.0218 0.0611 0.0699 0.0441 0.0377 0.0475 0.0337 0.0466 0.032 0.0486 0.0666 0.0556 0.0084 0.0433 0.0498 0.0621 0.0609 0.0723
Vacuole Periphery 0.0063 0.0065 0.0087 0.0069 0.0079 0.0059 0.0038 0.0028 0.0044 0.0069 0.0059 0.0044 0.0005 0.0022 0.0048 0.0046 0.0104 0.0035

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5252 9.1452 8.0787 11.3574 4.1055 9.7377 8.8064 9.9557 9.8507 9.1425
Translational Efficiency 0.4835 0.5141 0.6801 0.4085 0.8208 0.5186 0.6948 0.6571 0.7478 0.5856

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
284 772 1037 90 1537 1414 139 1469 1821 2186 1176 1559

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 893.84 679.19 902.00 1020.03 734.39 720.24 961.06 885.83 759.26 705.74 908.98 893.58
Standard Deviation 140.41 100.52 104.25 131.50 80.61 97.69 95.82 106.85 109.12 100.63 105.03 112.85
Intensity Change Log 2 — -0.396201 0.013111 0.190523 — -0.028069 0.388080 0.270483 — -0.218465 0.194370 0.227138

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001578 0.000802 0.000319 0.000671 0.000062 0.000257 0.000443 0.000316 0.000299 0.000449 0.000333 0.000337
Bud Neck 0.003686 0.019746 0.005758 0.003589 0.008408 0.020152 0.002344 0.010374 0.007671 0.020009 0.005354 0.009982
Bud Site 0.034326 0.007279 0.008374 0.014472 0.002206 0.007564 0.005755 0.014773 0.007216 0.007464 0.008064 0.014756
Cell Periphery 0.000527 0.000182 0.000129 0.000377 0.000192 0.000170 0.000284 0.000128 0.000244 0.000175 0.000147 0.000143
Cytoplasm 0.346724* 0.311986* 0.352971* 0.185015* 0.382286* 0.336757* 0.320332* 0.371213* 0.376739* 0.328009* 0.349113* 0.360464*
Cytoplasmic Foci 0.080777 0.311347* 0.046860 0.065790 0.248883* 0.302142* 0.067238 0.069047 0.222665* 0.305393* 0.049269 0.068859
Eisosomes 0.000117 0.000233 0.000051 0.000175 0.000256 0.000273 0.000137 0.000077 0.000234 0.000259 0.000061 0.000082
Endoplasmic Reticulum 0.007456 0.006983 0.015320 0.022367 0.018580 0.006856 0.018584 0.007405 0.016845 0.006901 0.015706 0.008269
Endosome 0.021724 0.059215 0.023739 0.023773 0.031218 0.045345 0.022031 0.022485 0.029738 0.050243 0.023537 0.022560
Golgi 0.008711 0.004705 0.000337 0.001505 0.005098 0.007158 0.000676 0.001254 0.005661 0.006292 0.000377 0.001269
Lipid Particles 0.003486 0.049395 0.008504 0.013540 0.059073 0.043919 0.015369 0.008325 0.050404 0.045853 0.009316 0.008626
Mitochondria 0.006755 0.005448 0.001467 0.000160 0.005200 0.011040 0.000229 0.000798 0.005442 0.009065 0.001321 0.000761
Mitotic Spindle 0.013114 0.003463 0.009626 0.024110 0.000689 0.001733 0.004463 0.010414 0.002627 0.002344 0.009015 0.011204
None 0.000781 0.003726 0.003204 0.000225 0.002273 0.002272 0.000956 0.002178 0.002040 0.002786 0.002939 0.002065
Nuclear Periphery 0.001961 0.003453 0.018698 0.002920 0.002766 0.002379 0.010467 0.005269 0.002640 0.002758 0.017725 0.005133
Nuclear Periphery Foci 0.002913 0.004210 0.015389 0.038064 0.001162 0.001327 0.028828 0.007012 0.001435 0.002345 0.016978 0.008804
Nucleolus 0.000443 0.003956 0.003108 0.000190 0.001066 0.001680 0.001175 0.001188 0.000969 0.002484 0.002880 0.001130
Nucleus 0.122112 0.073020 0.241973* 0.035512 0.109407 0.094263 0.180544* 0.098085 0.111389 0.086761 0.234712* 0.094472
Peroxisomes 0.001478 0.009589 0.001045 0.001060 0.002902 0.009598 0.001157 0.001483 0.002680 0.009595 0.001058 0.001459
Vacuole 0.336236* 0.116377 0.240139* 0.565930* 0.115264 0.101794 0.318487* 0.366228* 0.149727 0.106944 0.249399* 0.377756*
Vacuole Periphery 0.005096 0.004882 0.002990 0.000556 0.003009 0.003321 0.000501 0.001948 0.003335 0.003873 0.002696 0.001868

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.53 1.75 1.01 0.26 -2.85 -4.39 -3.20 -3.03 -0.07 1.36 -1.21 -1.80 -0.62 0.90 1.15
Bud Neck -6.95 -4.41 -0.25 6.73 3.39 -6.58 6.71 -0.77 5.78 -6.47 -8.41 2.27 -1.29 6.90 -3.17
Bud Site 3.35 3.20 1.76 -1.46 -1.32 -4.93 -1.63 -6.23 -2.51 -1.48 -1.07 -0.85 -3.45 -2.94 -2.25
Cell Periphery 2.42 3.07 0.51 -2.86 -3.91 1.75 -3.59 8.19 5.89 7.38 2.89 4.71 6.08 5.61 2.36
Cytoplasm 0.02 -1.39 4.11 4.66 5.73 5.08 3.56 2.41 -2.34 -2.47 5.59 4.01 2.36 -2.53 -1.60
Cytoplasmic Foci -27.42 4.42 1.31 27.67 -2.41 -7.39 24.51 36.44 41.32 -0.12 -13.81 37.26 33.39 51.17 -5.97
Eisosomes -6.29 4.73 -2.33 2.89 -5.50 -0.95 7.65 16.54 14.66 5.40 -1.81 17.43 15.93 16.77 -2.85
Endoplasmic Reticulum -0.65 -5.65 -2.82 -2.68 -0.45 6.90 0.65 7.92 0.59 5.53 6.89 0.89 7.10 0.00 10.18
Endosome -10.60 -2.50 -0.76 9.12 1.20 -5.49 3.76 7.36 10.97 0.68 -9.76 4.02 6.48 15.03 2.53
Golgi 0.27 1.72 1.47 4.47 -0.86 -2.40 8.08 7.76 7.74 -0.67 -1.41 8.19 6.34 9.00 -1.65
Lipid Particles -14.15 -7.80 -4.67 10.67 -1.52 4.62 14.55 20.90 16.50 3.61 1.63 18.89 19.92 20.61 2.27
Mitochondria -0.38 1.02 1.48 7.67 4.41 -3.90 7.31 6.59 7.81 -5.31 -3.45 4.95 6.07 9.39 2.35
Mitotic Spindle 1.64 -0.07 -0.37 -1.10 -0.36 -1.48 -0.89 -5.11 -4.34 -1.70 -0.11 -4.08 -4.13 -4.08 0.10
None -3.30 -3.19 2.25 4.00 4.26 0.00 2.13 0.63 0.76 -1.69 -1.10 -0.66 0.51 1.76 1.23
Nuclear Periphery -2.39 -11.97 -1.14 1.36 11.34 1.03 -2.99 -3.57 -3.89 1.65 -0.38 -11.96 -3.78 -3.39 8.97
Nuclear Periphery Foci -2.05 -10.21 -5.40 -5.08 -2.54 -0.80 -6.73 -9.72 -8.94 5.30 -3.62 -13.38 -10.56 -8.42 7.24
Nucleolus -6.16 -6.03 2.18 6.70 6.70 -2.37 -0.18 -0.12 2.47 0.09 -5.47 -4.75 -0.38 5.36 4.64
Nucleus 3.51 -9.90 6.42 5.86 23.87 3.18 -3.99 2.42 -0.79 4.70 5.63 -17.23 3.48 -1.88 19.35
Peroxisomes -8.80 0.29 0.66 9.26 0.69 -6.91 5.70 4.84 8.69 -0.55 -9.68 7.06 4.52 11.61 -0.76
Vacuole 15.29 7.97 -5.37 -17.23 -12.05 2.74 -10.79 -34.13 -36.18 -1.74 9.76 -11.96 -27.97 -38.75 -14.02
Vacuole Periphery -0.97 0.44 1.96 5.82 5.09 -0.89 10.99 4.16 4.85 -3.16 -1.86 1.54 4.07 5.89 2.11
ORF
Human Ortholog
Description Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene
Localization
Cell Percentages ER (19%), cytoplasm (10%), mixed (49%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YPR003C

YPR003C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypr003c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available