Standard name
Human Ortholog
Description 2-methylisocitrate lyase of the mitochondrial matrix; functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0.05 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.22 0.44 0.64 0.57 0.5 0.28 0.26 – 0.15 0.19 0.09 0.11 0.78 0.71 0.71 0.36 0.36 0.32 0.08 0.13 0.08 0.2 0.18 0.14
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0
Endosome 0 0.13 0 0.06 0.09 0.07 0.07 – 0 0 0 0 0 0.09 0.12 0.12 0.17 0.2 0.11 0.11 0.07 0.08 0.06 0.11
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.74 0.25 0 0.15 0.16 0.53 0.54 – 0.79 0.71 0.89 0.83 0 0 0 0.1 0.06 0.07 0.6 0.49 0.57 0.53 0.4 0.44
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0.08 0 0 0.09 0 0 0 0 0 0
SpindlePole 0.06 0.07 0.18 0.15 0.19 0.08 0.16 – 0.11 0.13 0.06 0.11 0.11 0.17 0.11 0.31 0.32 0.3 0.06 0.08 0.08 0.05 0.14 0.12
Vac/Vac Membrane 0.07 0.1 0.09 0 0.09 0.07 0.07 – 0 0 0 0 0 0 0 0.11 0.1 0.13 0.05 0.05 0.05 0.06 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 4 1 1 0 1 – 0 2 0 2 2 2 2 5 4 1 0 10 8 1 2 2
Bud 1 8 4 5 4 11 11 – 16 12 16 31 0 1 1 6 9 4 0 6 3 0 1 2
Bud Neck 0 0 20 5 2 3 5 – 1 9 4 7 0 1 3 3 6 5 1 5 5 0 2 1
Bud Site 0 0 0 1 0 1 1 – 2 1 11 11 0 0 0 6 1 2 – – – – – –
Cell Periphery 0 2 7 2 3 1 3 – 2 1 3 6 0 1 1 4 8 10 0 1 3 0 0 0
Cytoplasm 15 117 151 119 136 95 103 – 51 59 53 60 35 100 92 74 81 88 6 45 28 10 14 13
Endoplasmic Reticulum 1 10 2 5 3 2 2 – 0 2 3 6 0 2 2 12 15 17 0 3 3 0 0 0
Endosome 2 34 6 13 25 22 28 – 6 4 3 4 1 13 16 24 37 56 9 39 27 4 4 10
Golgi 0 1 0 2 1 0 0 – 0 0 0 0 0 0 0 4 1 4 0 6 2 0 0 1
Mitochondria 50 66 2 31 43 178 211 – 273 219 503 455 1 2 0 20 13 20 53 173 205 28 32 41
Nucleus 1 0 1 2 4 13 7 – 6 10 15 16 0 1 1 1 5 2 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 2 1 – 1 3 4 6 0 0 0 1 1 0 0 0 4 0 0 0
Nucleolus 1 0 0 0 1 0 1 – 0 0 3 3 0 0 0 0 2 2 0 1 1 0 0 0
Peroxisomes 0 15 2 6 6 3 6 – 1 1 0 2 1 6 10 6 5 24 3 6 9 0 1 0
SpindlePole 4 19 42 32 52 28 63 – 38 41 33 61 5 24 14 64 72 85 4 27 29 2 11 10
Vac/Vac Membrane 5 27 21 6 24 22 29 – 15 13 21 16 2 1 2 22 22 36 4 17 18 3 4 5
Unique Cell Count 68 263 235 208 272 338 390 347 309 563 550 45 140 129 208 223 279 89 354 361 53 81 95
Labelled Cell Count 80 301 262 230 305 381 472 412 377 672 686 47 154 144 252 282 356 89 354 361 53 81 95


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 4.7 5.3 5.1 5.5 4.7 4.8 – 4.1 4.3 3.8 4.0 5.4 5.5 5.1 6.2 6.3 6.0 4.4 5.7 5.5
Std Deviation (1e-4) 0.6 1.2 0.9 1.6 1.4 1.5 1.4 – 1.1 1.3 0.9 0.8 1.3 1.2 1.3 1.3 1.7 1.4 1.0 2.3 1.1
Intensity Change (Log2) – – – -0.07 0.04 -0.19 -0.16 – -0.37 -0.3 -0.5 -0.43 0.03 0.04 -0.08 0.21 0.24 0.17 -0.27 0.08 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 0.6 0.3 1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.406 -0.1152 -0.231 -0.5906 -0.539 -0.2652 0.027 -0.1076 -0.0809 -0.0846 0.1355 0.1088 0.284 -0.3631 -0.0138 0.3615 0.3782 -0.1726
Actin 0.0133 0.0084 0.008 0.0125 0.005 0.0054 0.0318 0.0061 0.023 0.013 0.019 0.0107 0.056 0.008 0.0429 0.0346 0.0509 0.0249
Bud 0.0019 0.0046 0.0028 0.0021 0.0048 0.0007 0.0046 0.0065 0.0027 0.0074 0.0025 0.0018 0.0016 0.0077 0.0067 0.0283 0.0172 0.0079
Bud Neck 0.0035 0.0026 0.0077 0.0005 0.0018 0.0033 0.0068 0.0023 0.0043 0.0023 0.0037 0.0067 0.0186 0.0038 0.0025 0.0008 0.0023 0.013
Bud Periphery 0.007 0.0099 0.0065 0.0094 0.0108 0.002 0.0177 0.0101 0.0105 0.0206 0.0073 0.0072 0.0032 0.0108 0.0168 0.0317 0.0328 0.0069
Bud Site 0.0072 0.0347 0.0126 0.0009 0.0021 0.0009 0.0116 0.027 0.0129 0.0136 0.0066 0.0022 0.0102 0.0388 0.0353 0.0116 0.0755 0.0024
Cell Periphery 0.0018 0.0016 0.0015 0.0007 0.0008 0.0007 0.0027 0.0023 0.0024 0.0029 0.0012 0.0009 0.0022 0.0014 0.0043 0.0013 0.0036 0.0013
Cytoplasm 0.0572 0.0671 0.0525 0.0787 0.0369 0.0642 0.0652 0.0801 0.0663 0.0735 0.046 0.0775 0.0855 0.1496 0.1308 0.1281 0.0505 0.1666
Cytoplasmic Foci 0.14 0.1053 0.1249 0.0765 0.1595 0.1473 0.1368 0.1221 0.1262 0.1461 0.1325 0.1343 0.1427 0.1494 0.1055 0.1041 0.0745 0.1499
Eisosomes 0.0013 0.0014 0.0008 0.0012 0.0008 0.0004 0.0014 0.0007 0.0016 0.0013 0.0011 0.0005 0.0007 0.0004 0.0016 0.0008 0.0002 0.0009
Endoplasmic Reticulum 0.0028 0.0038 0.0032 0.0057 0.0054 0.0018 0.0093 0.0043 0.0037 0.0035 0.0035 0.0026 0.0078 0.0043 0.0063 0.0066 0.0034 0.0073
Endosome 0.1097 0.115 0.1073 0.1016 0.0969 0.0929 0.1201 0.0868 0.0978 0.0734 0.1267 0.0713 0.1455 0.1474 0.1094 0.0726 0.2406 0.1176
Golgi 0.0241 0.022 0.0195 0.0211 0.0302 0.0201 0.0326 0.0335 0.0367 0.0405 0.0667 0.0287 0.0449 0.0461 0.0396 0.0644 0.0662 0.064
Lipid Particles 0.0416 0.0237 0.0264 0.005 0.0276 0.0316 0.0432 0.0135 0.0264 0.0331 0.0259 0.0314 0.071 0.0172 0.0147 0.0475 0.1236 0.0207
Mitochondria 0.2074 0.1704 0.1832 0.3548 0.1893 0.2333 0.1498 0.2342 0.2406 0.3029 0.2374 0.2303 0.1077 0.1135 0.1376 0.0506 0.1949 0.1608
None 0.3121 0.3273 0.3474 0.2872 0.279 0.3052 0.2999 0.2825 0.2695 0.1703 0.2326 0.2982 0.2307 0.2373 0.2592 0.318 0.0081 0.1598
Nuclear Periphery 0.0027 0.0031 0.0052 0.0027 0.0072 0.0016 0.0059 0.0018 0.0029 0.0047 0.0035 0.0016 0.0071 0.0015 0.0032 0.0037 0.0022 0.0021
Nucleolus 0.0031 0.0075 0.0022 0.0007 0.0087 0.0025 0.0018 0.0024 0.0017 0.0016 0.0017 0.0009 0.0014 0.0009 0.0035 0.0016 0.0005 0.004
Nucleus 0.0021 0.0065 0.0033 0.0021 0.0107 0.0028 0.0031 0.004 0.0018 0.0028 0.0019 0.0023 0.0026 0.0016 0.0041 0.0034 0.0013 0.0041
Peroxisomes 0.0442 0.0494 0.0644 0.0204 0.073 0.0651 0.035 0.0604 0.0537 0.0654 0.0532 0.0801 0.0372 0.0381 0.0256 0.0744 0.0306 0.0653
Punctate Nuclear 0.0036 0.0163 0.0056 0.0017 0.0349 0.0065 0.0045 0.0015 0.0029 0.0117 0.0086 0.0032 0.0069 0.0028 0.0164 0.0042 0.0011 0.0089
Vacuole 0.0103 0.015 0.0116 0.0088 0.0102 0.0086 0.0126 0.0141 0.0092 0.0067 0.0137 0.005 0.0109 0.0154 0.027 0.0088 0.0136 0.0083
Vacuole Periphery 0.0031 0.0043 0.0034 0.0057 0.0044 0.0028 0.0036 0.0036 0.0031 0.0027 0.0047 0.0025 0.0054 0.0043 0.0071 0.0032 0.0065 0.0032

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.536 12.558 6.7772 8.2322 10.3488 8.9347 9.6673 9.7385 11.2852 11.2708
Translational Efficiency 0.741 0.5938 1.0483 1.0397 0.7631 1.0043 0.8789 1.0128 0.9262 0.8176

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
239 1085 299 380 1466 1429 180 366 1705 2514 479 746

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 591.84 707.89 978.16 933.84 644.82 681.56 936.93 884.29 637.39 692.92 962.67 909.53
Standard Deviation 66.07 97.84 110.24 136.29 70.56 93.29 106.17 114.31 72.33 96.17 110.55 128.40
Intensity Change Log 2 — 0.258318 0.724863 0.657968 — 0.079944 0.539045 0.455623 — 0.168065 0.630963 0.556006

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000856 0.001452 0.005875 0.023783 0.000513 0.002977 0.012371 0.021313 0.000561 0.002319 0.008316 0.022571
Bud Neck 0.108921 0.039646 0.016678 0.014701 0.039432 0.042477 0.014590 0.015894 0.049173 0.041255 0.015894 0.015287
Bud Site 0.015724 0.038580 0.054758 0.129928* 0.012766 0.030858 0.066364 0.126168* 0.013181 0.034191 0.059120 0.128083*
Cell Periphery 0.000638 0.000283 0.000259 0.000355 0.000277 0.000355 0.000318 0.000297 0.000328 0.000324 0.000281 0.000327
Cytoplasm 0.188400* 0.100814* 0.111772* 0.045804 0.149222* 0.091557 0.134717* 0.067537 0.154714* 0.095552 0.120395* 0.056467
Cytoplasmic Foci 0.335821* 0.256987* 0.151236 0.146718 0.363982* 0.252987* 0.174579 0.140399 0.360035* 0.254713* 0.160008 0.143618
Eisosomes 0.000570 0.000193 0.000156 0.000148 0.000329 0.000250 0.000187 0.000132 0.000363 0.000225 0.000168 0.000140
Endoplasmic Reticulum 0.001155 0.000974 0.010752 0.001932 0.001946 0.000774 0.008234 0.001352 0.001835 0.000860 0.009806 0.001648
Endosome 0.042048 0.104681 0.083710 0.086148 0.056815 0.102685 0.082895 0.087887 0.054745 0.103546 0.083404 0.087001
Golgi 0.016733 0.061731 0.021684 0.093717 0.037944 0.070666 0.017023 0.082126 0.034971 0.066810 0.019932 0.088030
Lipid Particles 0.028806 0.010102 0.012679 0.004794 0.026628 0.016027 0.012483 0.003138 0.026933 0.013470 0.012606 0.003981
Mitochondria 0.084370 0.174539* 0.008055 0.088615 0.150428* 0.195602* 0.006915 0.094213 0.141168* 0.186512* 0.007627 0.091362
Mitotic Spindle 0.003275 0.025014 0.098890 0.161981* 0.004419 0.023590 0.094950 0.115733* 0.004259 0.024204 0.097409 0.139291*
None 0.002308 0.001325 0.000904 0.000221 0.001697 0.002228 0.000972 0.000201 0.001783 0.001838 0.000929 0.000211
Nuclear Periphery 0.000429 0.000293 0.002501 0.001104 0.000664 0.000416 0.003138 0.000293 0.000631 0.000363 0.002740 0.000706
Nuclear Periphery Foci 0.000245 0.000433 0.006804 0.001745 0.000296 0.000470 0.005278 0.000890 0.000289 0.000454 0.006231 0.001326
Nucleolus 0.001596 0.002447 0.004997 0.000378 0.002100 0.004189 0.003820 0.000794 0.002030 0.003437 0.004555 0.000582
Nucleus 0.072942 0.022870 0.106657 0.005906 0.043586 0.020491 0.100123 0.004445 0.047701 0.021518 0.104202 0.005189
Peroxisomes 0.064020 0.093010 0.059915 0.121493* 0.059822 0.082255 0.070717 0.163351* 0.060410 0.086897 0.063975 0.142029*
Vacuole 0.027065 0.054509 0.231925* 0.062968 0.039811 0.049317 0.184632 0.068295 0.038024 0.051558 0.214153* 0.065581
Vacuole Periphery 0.004077 0.010116 0.009791 0.007560 0.007323 0.009828 0.005692 0.005543 0.006868 0.009953 0.008250 0.006571

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.14 -5.57 -6.20 -6.07 -4.67 -2.33 -4.36 -4.69 -3.98 -1.91 -2.87 -7.04 -7.67 -6.85 -4.66
Bud Neck 5.40 7.31 7.39 6.96 0.57 -0.89 8.67 7.14 7.82 -0.82 2.38 11.23 10.74 10.62 -0.06
Bud Site -6.28 -6.13 -11.47 -9.03 -6.97 -6.72 -4.91 -11.75 -9.59 -4.82 -10.08 -8.32 -16.72 -13.26 -8.60
Cell Periphery 2.22 2.25 1.90 -0.87 -0.97 -1.60 -0.66 -0.23 1.19 0.34 0.15 1.07 0.47 0.34 -0.55
Cytoplasm 5.92 4.30 8.90 5.55 5.73 8.42 0.36 7.49 1.68 3.96 9.78 2.86 12.50 4.91 6.57
Cytoplasmic Foci 4.83 12.26 11.61 11.41 -0.96 13.61 15.19 23.72 12.71 1.63 15.01 25.13 28.46 17.02 0.37
Eisosomes 4.70 5.10 5.33 1.98 1.26 2.24 5.78 9.19 3.45 3.08 5.10 8.87 10.65 3.93 3.01
Endoplasmic Reticulum 0.37 -9.13 -1.80 -3.26 8.58 2.82 -6.39 0.38 -3.14 6.78 2.68 -10.47 -0.31 -4.62 10.89
Endosome -8.96 -4.30 -4.42 3.21 -0.55 -10.32 -1.40 -3.36 2.89 -0.92 -13.14 -3.33 -4.80 4.33 -1.06
Golgi -10.04 -0.10 -8.55 -2.77 -8.58 -8.81 7.71 -4.90 -0.48 -8.15 -10.80 7.29 -8.12 -2.21 -11.68
Lipid Particles 3.95 3.24 4.92 3.14 3.66 5.22 6.49 14.97 8.90 5.23 8.05 7.68 14.35 8.34 6.00
Mitochondria -5.97 6.23 0.41 7.44 -7.12 -4.85 22.42 5.46 8.97 -7.69 -5.75 23.32 6.57 11.82 -10.45
Mitotic Spindle -6.19 -7.85 -11.82 -10.00 -3.92 -8.44 -5.51 -10.06 -8.22 -1.71 -10.87 -9.62 -15.54 -12.95 -3.96
None 0.95 1.38 2.24 6.33 4.27 -1.21 1.45 5.10 5.19 2.71 -0.34 2.36 5.52 6.91 4.91
Nuclear Periphery 0.93 -4.66 -1.17 -1.41 2.10 1.57 -2.95 1.91 0.70 3.38 2.04 -5.02 -0.31 -1.21 4.08
Nuclear Periphery Foci -2.51 -8.02 -2.65 -2.29 5.29 -2.05 -8.29 -3.22 -2.27 6.86 -2.79 -10.77 -3.58 -2.97 7.96
Nucleolus -2.34 -3.99 3.10 5.15 5.44 -2.67 -1.38 2.75 4.35 2.49 -2.91 -3.45 3.92 6.11 5.66
Nucleus 5.74 -3.94 7.46 4.53 10.61 7.22 -3.92 12.89 6.37 6.47 8.84 -7.67 14.21 7.35 12.38
Peroxisomes -3.09 1.26 -4.24 -2.24 -5.80 -4.53 -0.07 -7.40 -5.55 -6.00 -6.39 0.96 -9.04 -5.79 -8.56
Vacuole -6.10 -16.93 -7.23 -4.47 9.40 -3.06 -10.67 -5.93 -4.83 6.47 -5.35 -19.48 -8.77 -6.65 11.51
Vacuole Periphery -5.76 -3.30 -2.18 2.35 0.95 -2.71 2.21 2.36 4.60 -0.42 -4.10 -0.01 1.07 4.62 0.87
Standard name
Human Ortholog
Description 2-methylisocitrate lyase of the mitochondrial matrix; functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol
Localization
Cell Percentages mitochondrion (56%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Icl2

Icl2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Icl2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available