Standard name
Human Ortholog
Description Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.12 0.06 0.12 0.11 0 0 0 0 0 0 0 0 0.08 0 0 0.23 0.17 0.21 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.98 0.98 0.94 0.93 0.95 0.86 0.85 0.73 0.84 1.0 0.98 0.99 0.93 0.93 0.94 0.94 0.92 0.88 0.87 0.87 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.07 0.07 0 0.23 0.21 0.36 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 1 1 0 0 2 0 0 0 2 0 0 0 1 2 1 2 4
Bud 2 5 2 3 3 4 3 1 8 12 13 10 7 1 1 1 0 0 0 0 0 0 0 2
Bud Neck 0 0 1 0 2 2 7 3 3 4 0 1 0 0 0 0 0 0 1 2 2 1 3 2
Bud Site 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 39 14 44 28 10 8 17 1 5 4 4 8 26 8 10 51 24 25 3 1 5 3 2 4
Cytoplasm 314 242 356 262 422 511 658 553 378 452 380 499 326 329 423 209 131 110 294 241 334 128 247 257
Endoplasmic Reticulum 1 0 0 0 2 3 2 1 1 1 0 2 0 2 3 9 3 3 7 0 1 3 11 7
Endosome 0 1 0 0 2 1 0 2 1 0 1 0 0 0 0 3 1 0 0 3 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 2 1
Mitochondria 2 4 0 2 2 38 46 24 99 110 186 95 1 2 0 4 2 2 1 2 7 3 5 6
Nucleus 0 0 1 0 2 3 9 10 2 3 10 5 0 0 0 0 2 1 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 3 1 2 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 1 1 1 3 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 0 0 1 3 9 4 17 8 17 23 20 0 5 1 5 3 3 0 2 15 4 4 3
Unique Cell Count 317 247 360 266 432 545 706 583 437 532 522 591 327 336 427 225 141 117 312 261 379 148 283 296
Labelled Cell Count 358 266 405 296 448 579 749 614 510 607 620 649 360 348 438 285 166 146 312 261 379 148 283 296


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 6.0 5.9 5.8 6.0 4.7 4.8 5.2 4.2 4.3 4.1 4.8 6.5 6.8 6.8 9.0 8.8 8.8 5.6 5.9 6.2
Std Deviation (1e-4) 0.7 0.7 0.5 0.8 0.9 1.0 0.7 1.0 0.9 0.8 0.6 0.9 0.8 0.9 1.0 1.3 1.3 1.4 0.8 1.0 1.0
Intensity Change (Log2) – – – -0.02 0.03 -0.32 -0.3 -0.18 -0.49 -0.45 -0.52 -0.28 0.14 0.2 0.21 0.61 0.58 0.58 -0.07 -0.0 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -5.5 0 0 -8.4 0 0 0 0 -1.8 -4.9 -5.5 3.3 1.4 2.4
Cytoplasm -0.4 -1.3 -3.8 -4.1 -3.2 -6.5 -7.0 -10.2 -7.2 1.2 -1.0 0.2 -3.9 -3.7 -3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.9 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.3 0 3.4 4.0 3.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5549 2.53 2.0348 1.5647 1.4057 2.1033 1.9067 3.1427 2.5211 2.4036 1.8968 2.4724 2.2671 3.3162 2.8564 2.2835 2.2121 2.8361
Actin 0.0122 0.0106 0.0146 0.0042 0.0564 0.0044 0.0255 0.0109 0.0077 0.0085 0.0053 0.0047 0.0265 0.0057 0.0072 0.0505 0.0107 0.0111
Bud 0.0004 0.0016 0.0006 0.0003 0.0052 0.0003 0.0017 0.0021 0.0012 0.0008 0.0021 0.0004 0.0014 0.0003 0.0003 0.0065 0.0014 0.0002
Bud Neck 0.0008 0.0011 0.0014 0.0007 0.0042 0.0028 0.0026 0.0006 0.0011 0.0007 0.0011 0.0042 0.0026 0.0004 0.001 0.0042 0.0043 0.0015
Bud Periphery 0.0005 0.0014 0.0008 0.0007 0.0086 0.0005 0.0027 0.0026 0.0014 0.0012 0.002 0.0007 0.002 0.0004 0.0003 0.008 0.0013 0.0002
Bud Site 0.0024 0.0236 0.0092 0.0013 0.0112 0.0002 0.006 0.0257 0.0097 0.0009 0.0074 0.0013 0.0126 0.0048 0.0075 0.0139 0.0168 0.0015
Cell Periphery 0.0029 0.0027 0.0025 0.0007 0.0013 0.0005 0.0018 0.0012 0.001 0.0005 0.0012 0.0007 0.0015 0.0012 0.0009 0.0027 0.0013 0.0005
Cytoplasm 0.8168 0.8691 0.865 0.8353 0.731 0.9297 0.817 0.8892 0.9008 0.8912 0.8348 0.9396 0.8467 0.9461 0.9333 0.7365 0.8075 0.9557
Cytoplasmic Foci 0.0163 0.0055 0.0071 0.0072 0.0235 0.005 0.0336 0.0202 0.0175 0.0049 0.025 0.0158 0.0172 0.0032 0.0045 0.04 0.0303 0.0015
Eisosomes 0.0003 0.0002 0.0003 0.0002 0.0002 0 0.0002 0.0001 0.0001 0 0 0 0.0001 0.0001 0 0.0012 0.0003 0
Endoplasmic Reticulum 0.0066 0.0018 0.0045 0.0037 0.0039 0.0024 0.0039 0.0018 0.0028 0.0011 0.0023 0.0018 0.0048 0.0024 0.0019 0.0053 0.0054 0.0041
Endosome 0.0121 0.0012 0.0049 0.0029 0.0299 0.0036 0.0186 0.0046 0.0079 0.0252 0.0584 0.0044 0.0071 0.0016 0.0085 0.0259 0.0069 0.0027
Golgi 0.0052 0.0006 0.0026 0.0006 0.0191 0.0009 0.0095 0.0019 0.0036 0.0147 0.0095 0.0009 0.0054 0.0018 0.0013 0.0261 0.01 0.0003
Lipid Particles 0.012 0.0002 0.0036 0.0032 0.0056 0.0011 0.0041 0.0003 0.0029 0.0002 0.0188 0.0004 0.0162 0.0003 0.0007 0.0174 0.0087 0.0002
Mitochondria 0.0034 0.0002 0.0168 0.0141 0.0146 0.0003 0.0077 0.0003 0.0059 0.0009 0.0068 0.0002 0.0019 0.0129 0.0005 0.0165 0.0224 0.0001
None 0.0996 0.0756 0.0584 0.106 0.062 0.0386 0.0431 0.0349 0.0276 0.0471 0.007 0.022 0.0215 0.0172 0.0223 0.0021 0.0588 0.0156
Nuclear Periphery 0.001 0.0004 0.0006 0.0004 0.0014 0.003 0.0055 0.0003 0.001 0.0002 0.0013 0.0002 0.0006 0.0001 0.0013 0.0006 0.0007 0.0004
Nucleolus 0.0002 0.0001 0.0002 0.0001 0.0011 0.0001 0.0002 0.0001 0.0002 0 0.0003 0 0.0002 0 0.0003 0.0009 0.0003 0.0003
Nucleus 0.0004 0.0013 0.0007 0.0006 0.0031 0.0025 0.0009 0.0007 0.0013 0.0004 0.0011 0.0004 0.0005 0.0003 0.0015 0.0007 0.0007 0.0015
Peroxisomes 0.0034 0.0002 0.0028 0.0164 0.0045 0.0003 0.0103 0.0008 0.003 0.0001 0.0083 0.0005 0.0162 0.0004 0.0034 0.0363 0.0096 0
Punctate Nuclear 0.0015 0.0007 0.0005 0.0004 0.0019 0.0015 0.001 0.0004 0.0017 0.0001 0.0039 0.0005 0.0136 0.0001 0.0008 0.0005 0.0011 0.0002
Vacuole 0.0017 0.0019 0.0025 0.0012 0.0088 0.002 0.0029 0.0013 0.0015 0.0011 0.0021 0.0009 0.0012 0.0007 0.002 0.0032 0.001 0.0021
Vacuole Periphery 0.0003 0.0001 0.0002 0.0001 0.0026 0.0002 0.0011 0.0001 0.0002 0.0003 0.0012 0.0001 0.0002 0.0001 0.0004 0.0009 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1134 13.2463 14.744 18.2572 14.066 16.3965 14.9479 18.402 16.0095 15.2494
Translational Efficiency 0.8559 0.8544 1.018 0.7657 0.8262 0.9044 0.817 0.7451 0.9711 0.7049

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1521 1254 106 1010 1083 856 205 156 2604 2110 311 1166

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 664.27 837.82 1192.32 1026.90 793.95 810.42 1113.18 1101.92 718.20 826.70 1140.15 1036.94
Standard Deviation 78.09 123.26 133.26 125.37 103.33 125.03 127.11 141.68 109.95 124.71 134.58 130.21
Intensity Change Log 2 — 0.334871 0.843930 0.628454 — 0.029622 0.487567 0.472899 — 0.176718 0.660871 0.545846

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001401 0.001265 0.003031 0.001669 0.000820 0.001655 0.000936 0.000992 0.001159 0.001423 0.001650 0.001578
Bud Neck 0.033675 0.014426 0.005306 0.004507 0.015152 0.031644 0.002507 0.002817 0.025971 0.021411 0.003461 0.004281
Bud Site 0.028059 0.035085 0.042778 0.065078 0.016577 0.044387 0.025443 0.024073 0.023284 0.038859 0.031352 0.059592
Cell Periphery 0.000762 0.000420 0.001262 0.000290 0.000987 0.000712 0.000445 0.000289 0.000855 0.000539 0.000723 0.000290
Cytoplasm 0.527276* 0.581423* 0.367544* 0.615679* 0.584255* 0.516602* 0.449712* 0.517573* 0.550974* 0.555126* 0.421706* 0.602553*
Cytoplasmic Foci 0.218289* 0.201286* 0.139766 0.064775 0.222336* 0.210461* 0.105574 0.066303 0.219972* 0.205008* 0.117228 0.064980
Eisosomes 0.001360 0.000462 0.000155 0.000072 0.001080 0.000926 0.000120 0.000074 0.001244 0.000650 0.000132 0.000072
Endoplasmic Reticulum 0.002211 0.001129 0.007563 0.000638 0.002480 0.001136 0.003210 0.001009 0.002323 0.001132 0.004694 0.000687
Endosome 0.022117 0.021602 0.017646 0.006391 0.017210 0.026708 0.007540 0.013613 0.020076 0.023673 0.010985 0.007357
Golgi 0.011908 0.007139 0.000716 0.006076 0.009250 0.007302 0.000246 0.013470 0.010803 0.007205 0.000406 0.007065
Lipid Particles 0.009288 0.003354 0.008485 0.000932 0.006757 0.006516 0.003686 0.002083 0.008236 0.004637 0.005321 0.001086
Mitochondria 0.005685 0.000652 0.000065 0.002654 0.000908 0.002092 0.000031 0.000244 0.003698 0.001236 0.000043 0.002332
Mitotic Spindle 0.000144 0.002964 0.000834 0.026534 0.000787 0.001474 0.000575 0.001294 0.000412 0.002360 0.000664 0.023158
None 0.003821 0.001785 0.000194 0.001010 0.002662 0.002173 0.000636 0.001742 0.003339 0.001942 0.000485 0.001108
Nuclear Periphery 0.000104 0.000053 0.000474 0.000202 0.000111 0.000083 0.000387 0.000247 0.000107 0.000065 0.000417 0.000208
Nuclear Periphery Foci 0.000579 0.001184 0.032465 0.002563 0.001784 0.000687 0.016014 0.006439 0.001080 0.000982 0.021621 0.003081
Nucleolus 0.000604 0.000237 0.000086 0.000152 0.000314 0.001111 0.000079 0.000073 0.000483 0.000591 0.000081 0.000141
Nucleus 0.035987 0.019354 0.038990 0.024558 0.029361 0.023676 0.033130 0.039797 0.033231 0.021107 0.035128 0.026597
Peroxisomes 0.004252 0.004237 0.000722 0.002412 0.002594 0.006668 0.000527 0.002605 0.003563 0.005223 0.000594 0.002438
Vacuole 0.090444 0.100788 0.331577* 0.170638 0.083739 0.112805 0.349071* 0.296829* 0.087655 0.105663 0.343108* 0.187521
Vacuole Periphery 0.002035 0.001157 0.000341 0.003171 0.000837 0.001182 0.000130 0.008434 0.001537 0.001167 0.000202 0.003876

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.30 -2.75 0.38 0.15 4.12 -3.19 -0.01 0.53 3.46 0.64 -0.89 -1.42 -0.03 1.49 2.22
Bud Neck 9.87 15.05 17.34 10.51 2.58 -6.48 11.33 8.33 11.49 -1.09 3.06 19.40 19.52 15.54 0.29
Bud Site -2.60 -2.25 -3.37 -1.22 0.11 -8.87 -1.57 -0.90 7.13 0.67 -7.63 -1.52 -4.78 0.63 -2.64
Cell Periphery 7.61 -3.00 13.11 7.83 6.79 3.27 10.48 13.16 6.97 4.14 7.26 3.15 18.71 9.81 7.35
Cytoplasm -5.91 9.05 0.42 5.02 -8.47 6.45 10.56 6.12 3.43 -1.31 -0.54 12.83 4.25 4.37 -8.95
Cytoplasmic Foci 2.76 8.35 34.37 28.02 6.50 1.72 18.31 26.86 21.44 5.41 3.25 19.45 45.14 35.86 8.24
Eisosomes 5.16 7.36 7.92 6.10 7.45 1.06 12.89 13.40 6.35 4.09 5.00 11.15 11.72 8.68 8.14
Endoplasmic Reticulum 3.12 -4.40 4.33 1.77 5.82 2.81 -1.26 1.69 -1.17 2.81 4.21 -4.14 5.48 1.20 7.29
Endosome 0.41 3.34 16.46 11.66 6.69 -4.63 8.41 6.61 9.53 1.14 -2.59 9.92 18.09 16.33 6.38
Golgi 5.16 18.07 8.36 4.00 -1.67 2.14 12.07 2.18 1.50 -1.02 5.43 21.81 8.14 4.61 -2.18
Lipid Particles 9.53 1.34 13.96 8.04 7.11 0.20 5.43 9.08 3.13 3.24 5.06 5.67 17.82 5.33 8.34
Mitochondria 4.45 4.96 3.78 -0.95 -2.23 -1.39 3.51 3.01 2.39 -2.33 3.31 5.45 3.53 0.52 -2.31
Mitotic Spindle -2.65 -2.35 -5.17 -3.82 -4.82 -0.61 -0.73 -1.54 -0.89 -0.83 -2.54 -1.48 -5.17 -4.17 -4.67
None 2.47 7.65 5.86 1.20 -5.28 0.49 4.43 1.29 0.33 -2.70 2.19 8.35 6.34 1.55 -3.80
Nuclear Periphery 3.90 -7.54 -6.91 -8.79 3.12 0.68 -6.42 -4.02 -4.28 -0.44 2.41 -9.54 -7.70 -9.22 2.89
Nuclear Periphery Foci -5.96 -9.80 -12.50 -7.93 9.02 7.39 -7.28 -3.87 -5.23 4.71 0.99 -11.77 -10.12 -10.50 10.38
Nucleolus 6.06 10.70 6.78 1.02 -2.63 -1.02 8.80 1.04 1.16 -1.06 -0.34 12.66 6.30 1.27 -2.73
Nucleus 9.78 -1.51 -2.98 -8.27 -0.17 2.78 -2.58 -3.65 -4.05 -2.77 9.43 -2.43 -5.05 -8.73 -2.02
Peroxisomes -0.08 11.32 6.55 5.36 -1.59 -5.34 10.49 2.46 5.89 -0.86 -3.76 14.90 6.45 7.98 -2.16
Vacuole -2.73 -12.19 -23.82 -22.08 3.80 -5.71 -18.27 -12.93 -11.59 0.92 -5.79 -21.87 -27.40 -24.51 6.28
Vacuole Periphery 4.47 10.61 1.64 -0.08 -1.80 -1.97 13.61 -0.75 -0.51 -1.27 2.80 13.93 0.54 -0.26 -2.36
Standard name
Human Ortholog
Description Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress
Localization
Cell Percentages cytoplasm (80%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

Haa1

Haa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Haa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available