Standard name
Human Ortholog
Description Guanine nucleotide dissociation stimulator for Sec4p; functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Bud Site 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Cytoplasm 0.98 0.99 – 0.98 0.93 0.89 0.77 0.72 – 0.72 0.73 0.71 0.98 0.98 – 0.97 0.95 0.94 0.96 0.94 – 0.95 0.88 0.85
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0.05 0 0 0 0 – 0 0 0
Endosome 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Golgi 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Mitochondria 0 0 – 0 0.05 0.12 0.12 0.08 – 0.16 0.24 0.24 0 0 – 0 0 0 0 0 – 0 0 0
Nucleus 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
Vac/Vac Membrane 0 0 – 0 0.06 0.06 0.18 0.22 – 0.17 0.11 0.13 0 0 – 0 0 0 0 0 – 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 – 1 0 0 0 1 – 0 0 0 0 0 – 1 3 2
Bud 0 0 – 1 0 7 10 8 – 8 10 8 1 9 – 0 0 0 0 0 – 0 6 6
Bud Neck 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 3
Bud Site 0 0 – 0 0 0 0 0 – 1 0 1 0 0 – 0 0 0 – – – – – –
Cell Periphery 1 2 – 4 5 6 19 10 – 15 13 11 4 3 – 3 6 3 0 0 – 0 0 0
Cytoplasm 63 232 – 546 697 747 935 760 – 702 538 706 337 475 – 211 202 180 97 229 – 159 338 440
Endoplasmic Reticulum 1 2 – 0 1 1 2 0 – 10 4 8 3 4 – 10 5 1 0 0 – 0 2 12
Endosome 0 0 – 1 1 3 4 4 – 0 0 1 0 0 – 1 0 1 1 0 – 0 5 8
Golgi 0 0 – 1 0 0 0 2 – 0 0 0 0 0 – 0 0 0 0 0 – 0 4 3
Mitochondria 0 0 – 0 34 101 149 83 – 159 177 240 0 2 – 5 3 5 0 1 – 0 3 8
Nucleus 0 3 – 2 4 3 23 32 – 23 22 31 6 2 – 2 7 6 0 4 – 0 2 3
Nuclear Periphery 1 0 – 0 1 2 2 6 – 6 0 12 0 0 – 0 0 0 0 0 – 0 0 1
Nucleolus 0 0 – 0 0 0 1 5 – 1 0 0 0 0 – 1 0 0 0 0 – 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0
SpindlePole 0 0 – 0 0 1 0 0 – 0 0 0 0 0 – 0 0 0 0 0 – 0 0 4
Vac/Vac Membrane 0 0 – 16 44 48 219 232 – 167 83 134 3 4 – 1 4 5 0 3 – 2 13 18
Unique Cell Count 64 235 558 753 837 1213 1051 977 742 999 344 487 218 213 192 102 245 169 387 520
Labelled Cell Count 66 239 571 787 919 1364 1142 1093 847 1152 354 500 234 227 201 102 245 169 387 520


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 8.9 – 7.7 7.0 6.6 6.4 7.1 – 6.2 6.7 6.7 9.3 7.8 – 9.7 10.0 10.2 9.3 9.5 9.5
Std Deviation (1e-4) 1.5 1.4 – 1.5 1.3 1.4 1.2 1.5 – 1.3 1.3 1.4 1.3 1.2 – 1.5 1.8 2.5 1.4 1.7 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0654 6.3795 5.6846 5.1733 5.5771 5.3862 3.2948 5.3671 4.5354 3.8993 4.0448 4.1839 2.673 4.789 3.7999 3.6894 3.0888 3.7457
Actin 0.0289 0.0003 0.003 0.0034 0.0005 0.0026 0.0132 0.0001 0.001 0.0017 0.0003 0.0011 0.0094 0.0008 0.0005 0.0004 0.0002 0.0003
Bud 0.0008 0.0001 0.0013 0.0009 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0
Bud Neck 0.0097 0.0003 0.0006 0.0015 0.001 0.0013 0.0012 0.0001 0.0003 0.0004 0.0004 0.0006 0.0003 0.0002 0.0002 0.0003 0.0004 0.0008
Bud Periphery 0.0007 0 0.0006 0.0003 0 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0 0 0 0
Bud Site 0.0064 0.0007 0.005 0.0008 0.0001 0.0002 0.0009 0.0001 0.0003 0.0001 0 0 0.0003 0.0012 0.0002 0.0001 0.0001 0
Cell Periphery 0.0002 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8205 0.9535 0.9432 0.8761 0.9135 0.9253 0.9115 0.9852 0.9342 0.9738 0.9796 0.96 0.9052 0.9846 0.9714 0.987 0.96 0.9729
Cytoplasmic Foci 0.0164 0.0041 0.0089 0.0281 0.0066 0.0063 0.009 0.0011 0.0075 0.0033 0.0024 0.0061 0.0184 0.002 0.0022 0.0017 0.0031 0.0025
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0066 0.0016 0.0015 0.0043 0.0017 0.0027 0.003 0.0009 0.0027 0.003 0.001 0.0018 0.0031 0.0005 0.002 0.0007 0.0032 0.0016
Endosome 0.0125 0.0014 0.0027 0.0098 0.0023 0.0057 0.0053 0.0003 0.0049 0.0018 0.0018 0.0021 0.0089 0.0003 0.0007 0.0004 0.0012 0.0014
Golgi 0.0031 0.0001 0.0005 0.0015 0.0001 0.0013 0.0012 0 0.0014 0.0002 0.0001 0.0003 0.0026 0 0.0001 0 0.0001 0.0001
Lipid Particles 0.0029 0.0001 0.0002 0.0025 0 0.0047 0.0009 0 0.0018 0.0001 0 0 0.0063 0 0 0 0 0
Mitochondria 0.0007 0.0001 0.0072 0.0006 0.0002 0.0009 0.0003 0 0.0005 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001
None 0.0574 0.0193 0.0083 0.0138 0.0089 0.0099 0.0377 0.0088 0.036 0.0071 0.0045 0.0163 0.0224 0.0038 0.0153 0.0023 0.0068 0.0026
Nuclear Periphery 0.0071 0.0015 0.0015 0.0052 0.0024 0.0032 0.0022 0.0004 0.0012 0.0019 0.0007 0.001 0.0028 0.0006 0.001 0.0008 0.0024 0.0012
Nucleolus 0.0011 0 0.0001 0.0004 0.0001 0.0001 0.0004 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Nucleus 0.0155 0.0152 0.0128 0.0242 0.0586 0.0259 0.0073 0.0023 0.0039 0.0048 0.0084 0.0086 0.0049 0.0047 0.0053 0.0054 0.021 0.0157
Peroxisomes 0.0025 0.0001 0.0004 0.0015 0.0002 0.0019 0.0003 0 0.0024 0.0001 0 0.0007 0.0096 0.0002 0 0 0.0001 0
Punctate Nuclear 0.0049 0.0009 0.0014 0.0232 0.003 0.0068 0.0042 0.0001 0.0011 0.0009 0.0004 0.0008 0.0042 0.0003 0.0004 0.0003 0.0008 0.0006
Vacuole 0.0017 0.0005 0.0006 0.0016 0.0005 0.0006 0.0006 0.0002 0.0006 0.0003 0.0002 0.0003 0.0005 0.0002 0.0003 0.0002 0.0004 0.0003
Vacuole Periphery 0.0004 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 40.8668 20.5495 33.8858 27.6512 19.8696 45.9497 17.3147 29.6051 30.9072 27.8375
Translational Efficiency 0.971 1.3034 0.6894 1.2654 1.4872 1.3239 1.8136 0.9046 1.1872 1.3777

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
266 447 2057 1170 2005 1543 2419 35 2271 1990 4476 1205

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 756.69 867.87 967.36 1089.92 846.37 873.98 956.59 1135.40 835.87 872.61 961.54 1091.24
Standard Deviation 92.78 165.12 138.95 168.87 116.16 116.87 131.96 154.63 117.28 129.31 135.33 168.65
Intensity Change Log 2 — 0.197777 0.354351 0.526448 — 0.046312 0.176612 0.423840 — 0.119792 0.263243 0.473184

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000139 0.000345 0.000132 0.004184 0.000109 0.000253 0.000426 0.000313 0.000113 0.000274 0.000291 0.004072
Bud Neck 0.005428 0.001625 0.003172 0.011222 0.002750 0.003609 0.006629 0.001205 0.003063 0.003163 0.005040 0.010931
Bud Site 0.006980 0.007191 0.007888 0.045060 0.004303 0.009867 0.008010 0.005056 0.004616 0.009266 0.007954 0.043899
Cell Periphery 0.000182 0.000126 0.000106 0.000153 0.000062 0.000066 0.000044 0.000159 0.000076 0.000079 0.000072 0.000153
Cytoplasm 0.566686* 0.737873* 0.672337* 0.508877* 0.733024* 0.755817* 0.748632* 0.619744* 0.713541* 0.751786* 0.713570* 0.512097*
Cytoplasmic Foci 0.237817* 0.065661 0.015339 0.018950 0.094672 0.087684 0.006247 0.027080 0.111439 0.082737 0.010425 0.019186
Eisosomes 0.000085 0.000030 0.000027 0.000031 0.000024 0.000023 0.000008 0.000065 0.000032 0.000024 0.000017 0.000032
Endoplasmic Reticulum 0.002254 0.001803 0.000819 0.001277 0.001582 0.000793 0.000424 0.001447 0.001661 0.001020 0.000606 0.001282
Endosome 0.010276 0.005362 0.002052 0.015171 0.004603 0.007870 0.001329 0.006547 0.005267 0.007307 0.001661 0.014921
Golgi 0.001920 0.002225 0.000220 0.015185 0.000707 0.003619 0.001118 0.000119 0.000849 0.003306 0.000706 0.014748
Lipid Particles 0.003389 0.001736 0.000707 0.002243 0.001346 0.001254 0.000269 0.001711 0.001585 0.001362 0.000470 0.002228
Mitochondria 0.000542 0.000707 0.000417 0.013173 0.000553 0.001243 0.001645 0.000063 0.000552 0.001123 0.001081 0.012793
Mitotic Spindle 0.000266 0.000886 0.015529 0.111403* 0.000932 0.004144 0.015074 0.001148 0.000854 0.003412 0.015283 0.108200*
None 0.001302 0.007729 0.006593 0.010579 0.003606 0.003581 0.007469 0.001402 0.003336 0.004513 0.007066 0.010312
Nuclear Periphery 0.000247 0.000294 0.000695 0.000716 0.000206 0.000156 0.000314 0.000485 0.000211 0.000187 0.000490 0.000709
Nuclear Periphery Foci 0.001770 0.004289 0.001497 0.002456 0.001427 0.001039 0.001067 0.004686 0.001467 0.001769 0.001265 0.002520
Nucleolus 0.000515 0.000727 0.000326 0.001244 0.000399 0.000710 0.000208 0.000211 0.000413 0.000714 0.000262 0.001214
Nucleus 0.083896 0.088437 0.184735* 0.136106* 0.089150 0.054977 0.169909* 0.104560 0.088535 0.062493 0.176723* 0.135189*
Peroxisomes 0.001415 0.000809 0.000306 0.004407 0.000966 0.001744 0.000327 0.000218 0.001018 0.001534 0.000318 0.004285
Vacuole 0.073673 0.070091 0.086602 0.088884 0.059103 0.060060 0.030699 0.223583* 0.060810 0.062314 0.056390 0.092796
Vacuole Periphery 0.001217 0.002053 0.000498 0.008679 0.000475 0.001491 0.000150 0.000198 0.000562 0.001617 0.000310 0.008432

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.15 -1.74 -4.58 -3.50 -4.38 -2.15 -2.02 -3.72 -2.25 -1.55 -3.17 -2.55 -4.76 -3.94 -4.03
Bud Neck 4.43 2.80 -1.17 -5.11 -3.75 -1.69 -2.70 3.57 4.99 4.51 -0.25 -2.08 -3.94 -3.79 -2.54
Bud Site -0.34 0.70 -4.15 -4.10 -4.86 -4.16 -1.80 -2.28 1.27 -1.08 -4.33 -1.81 -6.12 -3.09 -5.27
Cell Periphery 2.66 5.08 2.88 0.01 -5.29 -0.58 3.89 -3.37 -3.20 -3.83 -0.53 2.52 -7.48 -6.20 -9.52
Cytoplasm -8.65 -2.14 8.59 22.86 18.62 -2.54 8.86 6.83 7.23 5.56 -4.70 12.19 32.41 34.94 25.77
Cytoplasmic Foci 13.68 18.99 19.22 12.41 2.91 1.56 30.04 15.19 13.29 -3.68 7.06 33.09 32.37 26.40 -0.76
Eisosomes 7.53 9.08 8.14 -0.06 -3.48 0.95 10.49 -4.03 -4.18 -5.39 3.81 9.58 -0.47 -4.30 -10.21
Endoplasmic Reticulum 0.79 2.13 1.76 1.89 -1.92 1.75 2.84 -0.38 -2.38 -3.95 1.63 2.56 0.92 -1.43 -6.01
Endosome 4.65 9.11 6.94 2.87 -1.98 -4.17 10.79 0.55 2.58 -2.27 -3.04 13.14 3.97 5.57 -3.07
Golgi -0.06 2.93 0.10 0.11 -2.37 -3.56 0.79 8.09 4.30 1.27 -3.28 2.94 -1.36 1.41 -2.07
Lipid Particles 3.24 6.99 2.92 -1.32 -8.94 0.31 9.18 -1.40 -1.17 -4.39 0.83 10.09 -3.70 -2.79 -10.36
Mitochondria -0.34 0.89 -1.92 -1.71 -2.10 -1.97 -1.05 3.56 3.66 2.56 -1.96 -0.69 -1.90 -1.12 -1.67
Mitotic Spindle -2.15 -6.82 -9.96 -9.77 -6.17 -3.03 -7.11 -1.47 -0.63 1.42 -3.06 -9.45 -9.81 -9.01 -6.47
None -3.64 -11.26 -12.41 -1.52 -4.74 0.09 -8.84 2.60 2.37 8.85 -1.66 -9.00 -8.76 -6.45 -4.01
Nuclear Periphery -0.66 -18.82 -20.50 -16.48 -8.83 1.54 -14.73 -4.98 -5.43 -1.01 0.72 -22.95 -21.77 -22.05 -12.96
Nuclear Periphery Foci -4.85 -0.39 -3.44 2.50 -4.71 2.37 1.88 -3.45 -3.70 -3.65 -2.41 -0.25 -7.04 -5.12 -6.93
Nucleolus -1.09 2.58 -1.08 0.22 -3.38 -1.25 1.81 3.36 2.17 1.79 -1.48 2.30 -2.13 0.33 -3.48
Nucleus -0.95 -18.56 -13.11 -12.84 5.55 11.72 -28.43 -0.38 -2.64 7.58 8.93 -36.36 -18.01 -23.29 6.27
Peroxisomes 2.36 6.11 1.64 -0.22 -2.53 -1.92 2.89 3.07 4.70 0.50 -1.56 3.56 0.42 1.85 -2.52
Vacuole -0.60 -6.89 -20.36 -21.26 -17.84 -1.17 -3.53 -7.67 -7.58 -7.42 -1.81 -11.59 -28.50 -27.36 -23.73
Vacuole Periphery -0.62 3.06 0.30 0.73 -1.56 -2.31 6.89 5.74 3.15 -1.14 -2.47 4.71 -1.04 0.83 -1.84
Standard name
Human Ortholog
Description Guanine nucleotide dissociation stimulator for Sec4p; functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dss4

Dss4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dss4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available