Standard name | |
---|---|
Human Ortholog | |
Description | Essential helicase component of heterohexameric MCM2-7 complexes; MCM2-7 complexes bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 1 | 5 | 4 | 4 | 7 | 7 | 12 | 13 | – | 11 | 13 | 0 | 0 | 1 | 0 | 1 | 0 | 13 | 4 | 5 | 7 | 7 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | – | 1 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 6 | 4 | – | 3 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 4 | 2 | – | 1 | 5 | 0 | 0 | 1 | 0 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 34 | 10 | 36 | 74 | 152 | 107 | 157 | 107 | 60 | – | 57 | 45 | 20 | 60 | 75 | 147 | 75 | 111 | 18 | 14 | 34 | 18 | 4 | 0 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 2 | – | 1 | 1 | 0 | 0 | 2 | 10 | 6 | 4 | 0 | 0 | 1 | 1 | 1 | 3 |
Endosome | 9 | 1 | 3 | 5 | 33 | 11 | 16 | 19 | 5 | – | 2 | 4 | 12 | 16 | 14 | 7 | 5 | 16 | 4 | 2 | 2 | 1 | 13 | 31 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 2 | 4 | 10 | 42 | 33 | 91 | 128 | 99 | 103 | – | 130 | 193 | 2 | 5 | 27 | 6 | 6 | 6 | 0 | 0 | 1 | 0 | 2 | 4 |
Nucleus | 66 | 40 | 68 | 169 | 140 | 94 | 198 | 234 | 126 | – | 151 | 171 | 180 | 220 | 133 | 158 | 104 | 110 | 57 | 52 | 68 | 95 | 154 | 105 |
Nuclear Periphery | 1 | 0 | 1 | 2 | 13 | 15 | 25 | 29 | 21 | – | 21 | 11 | 5 | 13 | 19 | 8 | 1 | 10 | 1 | 0 | 0 | 0 | 1 | 7 |
Nucleolus | 16 | 14 | 8 | 36 | 55 | 95 | 132 | 135 | 96 | – | 101 | 166 | 25 | 72 | 80 | 31 | 11 | 36 | 35 | 4 | 11 | 25 | 70 | 103 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 5 | 5 | 1 | 3 | 3 | – | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 6 | 6 | 0 | 2 | 1 | 9 | 21 |
Vac/Vac Membrane | 10 | 1 | 6 | 15 | 42 | 11 | 31 | 27 | 17 | – | 28 | 31 | 8 | 27 | 28 | 61 | 29 | 53 | 3 | 1 | 3 | 6 | 38 | 60 |
Unique Cell Count | 130 | 59 | 122 | 297 | 411 | 344 | 560 | 560 | 342 | 379 | 471 | 234 | 369 | 334 | 380 | 219 | 317 | 144 | 85 | 133 | 165 | 311 | 350 | |
Labelled Cell Count | 143 | 71 | 137 | 349 | 482 | 440 | 702 | 682 | 454 | 510 | 662 | 252 | 413 | 382 | 429 | 244 | 359 | 144 | 85 | 133 | 165 | 311 | 350 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.8 | 9.1 | 7.0 | 7.3 | 7.3 | 6.9 | 6.9 | 7.3 | 6.7 | – | 6.5 | 6.3 | 11.0 | 10.5 | 9.0 | 11.4 | 13.5 | 13.4 | 9.9 | 9.9 | 11.0 |
Std Deviation (1e-4) | 2.1 | 2.1 | 1.4 | 1.9 | 1.7 | 1.7 | 1.6 | 2.2 | 2.3 | – | 1.7 | 1.6 | 2.7 | 2.1 | 2.0 | 3.2 | 3.9 | 4.2 | 2.1 | 2.3 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.06 | -0.04 | -0.03 | 0.05 | -0.08 | – | -0.11 | -0.15 | 0.64 | 0.57 | 0.36 | 0.7 | 0.94 | 0.93 | 0.5 | 0.48 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | 1.5 | 0.3 | -0.3 | -2.6 | -2.8 | – | -3.6 | -5.7 | -5.2 | -3.2 | -1.6 | 1.8 | 0.9 | 1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.2 | 0 | 0 | 0 | 0 | 1.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.7 | -0.1 | 4.2 | 3.7 | 2.6 | 4.8 | – | 5.6 | 6.8 | 0 | 0 | 0 | 0 | -2.3 | 0 |
Nucleus | 0.2 | -4.3 | -5.7 | -4.2 | -2.8 | -3.6 | – | -3.1 | -3.9 | 4.1 | 0.8 | -3.0 | -2.7 | -1.5 | -4.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2.1 | 2.4 | – | 2.2 | 0 | 0 | 0 | 2.2 | 0 | 0 | 0 |
Nucleolus | 1.7 | 2.1 | 4.8 | 4.2 | 4.3 | 4.9 | – | 4.7 | 6.2 | 1.3 | 3.4 | 4.2 | 0.6 | -0.6 | 1.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 1.8 | 0 | 0.3 | 0 | 0 | – | 0.9 | 0.7 | 0 | 0.9 | 1.2 | 3.1 | 2.4 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.9191 | 7.4645 | 6.282 | 5.7585 | 6.0864 | 6.8658 | 8.8444 | 9.2233 | 7.6604 | 6.9333 | 7.4017 | 8.6862 | 5.7557 | 5.7759 | 4.6177 | 4.4691 | 5.1086 | 5.3642 |
Actin | 0.0232 | 0.0011 | 0.007 | 0.0083 | 0.0378 | 0.0004 | 0.0005 | 0.001 | 0.0139 | 0.0155 | 0.0021 | 0.0009 | 0.0191 | 0.0017 | 0.0083 | 0.0154 | 0.0035 | 0.0008 |
Bud | 0.0006 | 0.0209 | 0.0645 | 0.0315 | 0.0075 | 0.0106 | 0.0007 | 0.0228 | 0.0788 | 0.0195 | 0.0394 | 0.0033 | 0.0005 | 0.0079 | 0.0597 | 0.1215 | 0.0417 | 0.0031 |
Bud Neck | 0.0015 | 0.0024 | 0.0054 | 0.0072 | 0.0365 | 0.0084 | 0.0002 | 0.0014 | 0.0047 | 0.0115 | 0.0031 | 0.005 | 0.0011 | 0.0021 | 0.0055 | 0.0056 | 0.0033 | 0.0053 |
Bud Periphery | 0.0008 | 0.0112 | 0.0452 | 0.0304 | 0.007 | 0.0165 | 0.0015 | 0.0118 | 0.0511 | 0.0086 | 0.0217 | 0.0023 | 0.001 | 0.0054 | 0.0512 | 0.0987 | 0.0417 | 0.004 |
Bud Site | 0.0017 | 0.0551 | 0.0392 | 0.0348 | 0.0103 | 0.003 | 0.0006 | 0.0326 | 0.0322 | 0.0073 | 0.0053 | 0.001 | 0.0016 | 0.0154 | 0.0318 | 0.0247 | 0.0094 | 0.0028 |
Cell Periphery | 0.0003 | 0.0024 | 0.0033 | 0.0047 | 0.002 | 0.0011 | 0.0001 | 0.0014 | 0.0022 | 0.0009 | 0.0004 | 0.0003 | 0.0004 | 0.0012 | 0.0032 | 0.0026 | 0.0011 | 0.0004 |
Cytoplasm | 0.017 | 0.1103 | 0.3355 | 0.5402 | 0.5202 | 0.0028 | 0.0083 | 0.0936 | 0.3095 | 0.5655 | 0.6077 | 0.066 | 0.0038 | 0.1151 | 0.3599 | 0.477 | 0.6716 | 0.0622 |
Cytoplasmic Foci | 0.0262 | 0.0046 | 0.0244 | 0.0311 | 0.0286 | 0.0001 | 0.0033 | 0.005 | 0.0265 | 0.0551 | 0.0142 | 0.0015 | 0.0039 | 0.0035 | 0.0329 | 0.0236 | 0.022 | 0.0006 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0055 | 0.0106 | 0.0047 | 0.0067 | 0.0066 | 0.0001 | 0.0013 | 0.0018 | 0.0047 | 0.0058 | 0.0167 | 0.0014 | 0.001 | 0.0024 | 0.0122 | 0.0077 | 0.004 | 0.0008 |
Endosome | 0.0425 | 0.0088 | 0.0152 | 0.0358 | 0.0529 | 0.0002 | 0.0092 | 0.0036 | 0.0215 | 0.0613 | 0.0145 | 0.0071 | 0.0066 | 0.003 | 0.0234 | 0.0237 | 0.023 | 0.0018 |
Golgi | 0.0067 | 0.0013 | 0.0022 | 0.0026 | 0.0124 | 0.0001 | 0.0004 | 0.0003 | 0.0053 | 0.0127 | 0.0011 | 0.002 | 0.0012 | 0.0004 | 0.0024 | 0.0014 | 0.0011 | 0.0001 |
Lipid Particles | 0.0132 | 0.0008 | 0.0092 | 0.0132 | 0.0331 | 0.0001 | 0.003 | 0.0007 | 0.0039 | 0.03 | 0.0007 | 0.0011 | 0.0033 | 0.0007 | 0.0088 | 0.0012 | 0.0004 | 0.0001 |
Mitochondria | 0.0045 | 0.0011 | 0.0129 | 0.0283 | 0.011 | 0.0008 | 0.0017 | 0.0021 | 0.008 | 0.004 | 0.0012 | 0.0071 | 0.0029 | 0.0013 | 0.0042 | 0.0036 | 0.0016 | 0.0009 |
None | 0.0037 | 0.0009 | 0.0063 | 0.0052 | 0.0031 | 0.0001 | 0.0029 | 0.0012 | 0.0029 | 0.0033 | 0.0021 | 0.0008 | 0.0012 | 0.0014 | 0.0049 | 0.0055 | 0.0046 | 0.0003 |
Nuclear Periphery | 0.0143 | 0.0064 | 0.0222 | 0.0074 | 0.0182 | 0.0017 | 0.0298 | 0.0065 | 0.022 | 0.0099 | 0.0086 | 0.0052 | 0.0082 | 0.0365 | 0.0185 | 0.0117 | 0.0058 | 0.0036 |
Nucleolus | 0.0818 | 0.0974 | 0.0449 | 0.0479 | 0.035 | 0.2296 | 0.0313 | 0.04 | 0.0238 | 0.0084 | 0.0213 | 0.1092 | 0.0384 | 0.0426 | 0.0318 | 0.0105 | 0.0244 | 0.1393 |
Nucleus | 0.7203 | 0.6431 | 0.2798 | 0.0771 | 0.0406 | 0.7132 | 0.8965 | 0.7228 | 0.3198 | 0.108 | 0.1729 | 0.779 | 0.8883 | 0.7024 | 0.2412 | 0.1042 | 0.0894 | 0.7653 |
Peroxisomes | 0.0035 | 0.0002 | 0.0102 | 0.0024 | 0.0232 | 0.0001 | 0.0001 | 0.0003 | 0.0049 | 0.002 | 0.0002 | 0.0002 | 0.0044 | 0.0002 | 0.0173 | 0.0013 | 0.0005 | 0.0001 |
Punctate Nuclear | 0.0228 | 0.0042 | 0.02 | 0.0061 | 0.0126 | 0.0062 | 0.0036 | 0.0147 | 0.0185 | 0.0205 | 0.0518 | 0.0017 | 0.0096 | 0.0465 | 0.0412 | 0.0066 | 0.0029 | 0.005 |
Vacuole | 0.0073 | 0.0163 | 0.0421 | 0.0729 | 0.092 | 0.0042 | 0.0033 | 0.0306 | 0.0387 | 0.042 | 0.0133 | 0.0038 | 0.0028 | 0.0087 | 0.0363 | 0.0468 | 0.0429 | 0.0029 |
Vacuole Periphery | 0.0021 | 0.0011 | 0.0056 | 0.0061 | 0.009 | 0.0005 | 0.0015 | 0.0058 | 0.007 | 0.0081 | 0.0017 | 0.0013 | 0.0007 | 0.0016 | 0.0054 | 0.0065 | 0.0052 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.5703 | 12.6729 | 28.4728 | 43.2682 | 22.465 | 24.7951 | 18.0636 | 30.6294 | 37.2433 | 30.3152 |
Translational Efficiency | 0.7872 | 0.613 | 1.1431 | 0.7926 | 0.7621 | 0.676 | 0.8404 | 0.7217 | 0.7997 | 0.7421 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1741 | 1081 | 444 | 428 | 1620 | 1593 | 651 | 76 | 3361 | 2674 | 1095 | 504 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 919.39 | 1044.59 | 1544.27 | 1459.19 | 977.04 | 1033.46 | 1351.83 | 1503.73 | 947.18 | 1037.96 | 1429.86 | 1465.91 |
Standard Deviation | 135.06 | 206.20 | 220.46 | 226.82 | 183.77 | 192.84 | 233.99 | 229.25 | 162.96 | 198.42 | 247.36 | 227.75 |
Intensity Change Log 2 | — | 0.184188 | 0.748176 | 0.666419 | — | 0.080993 | 0.468424 | 0.622056 | — | 0.131944 | 0.610825 | 0.643734 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000567 | 0.001430 | 0.001309 | 0.000563 | 0.000706 | 0.000906 | 0.001011 | 0.001224 | 0.000634 | 0.001118 | 0.001132 | 0.000662 |
Bud Neck | 0.029198 | 0.030626 | 0.037813 | 0.005611 | 0.039502 | 0.043580 | 0.025556 | 0.008300 | 0.034165 | 0.038343 | 0.030526 | 0.006016 |
Bud Site | 0.017529 | 0.019588 | 0.017730 | 0.034203 | 0.016699 | 0.014096 | 0.019515 | 0.027144 | 0.017129 | 0.016317 | 0.018791 | 0.033139 |
Cell Periphery | 0.001603 | 0.000721 | 0.000524 | 0.000514 | 0.000769 | 0.000545 | 0.000444 | 0.000489 | 0.001201 | 0.000616 | 0.000477 | 0.000510 |
Cytoplasm | 0.149865* | 0.165863* | 0.203528* | 0.616577* | 0.118924* | 0.115948* | 0.293818* | 0.691709* | 0.134951* | 0.136127* | 0.257207* | 0.627906* |
Cytoplasmic Foci | 0.017264 | 0.022944 | 0.035042 | 0.053912 | 0.017348 | 0.010757 | 0.045299 | 0.065444 | 0.017304 | 0.015684 | 0.041140 | 0.055651 |
Eisosomes | 0.000059 | 0.000045 | 0.000044 | 0.000008 | 0.000048 | 0.000047 | 0.000030 | 0.000020 | 0.000053 | 0.000046 | 0.000036 | 0.000009 |
Endoplasmic Reticulum | 0.003707 | 0.003249 | 0.001584 | 0.000284 | 0.002884 | 0.002959 | 0.002592 | 0.000335 | 0.003310 | 0.003076 | 0.002183 | 0.000291 |
Endosome | 0.005303 | 0.006944 | 0.007151 | 0.006852 | 0.005047 | 0.004444 | 0.007272 | 0.004378 | 0.005179 | 0.005455 | 0.007223 | 0.006479 |
Golgi | 0.001182 | 0.003495 | 0.001619 | 0.001265 | 0.001148 | 0.001335 | 0.003363 | 0.000841 | 0.001166 | 0.002208 | 0.002656 | 0.001201 |
Lipid Particles | 0.000668 | 0.000878 | 0.002894 | 0.007068 | 0.000678 | 0.000834 | 0.001688 | 0.004441 | 0.000673 | 0.000851 | 0.002177 | 0.006672 |
Mitochondria | 0.002007 | 0.003013 | 0.002199 | 0.000247 | 0.002187 | 0.003365 | 0.003737 | 0.000314 | 0.002093 | 0.003223 | 0.003113 | 0.000257 |
Mitotic Spindle | 0.006928 | 0.019258 | 0.054534 | 0.139267* | 0.015523 | 0.026752 | 0.092568 | 0.096994 | 0.011071 | 0.023723 | 0.077146 | 0.132893* |
None | 0.007713 | 0.007044 | 0.003069 | 0.002062 | 0.007726 | 0.008373 | 0.007544 | 0.002219 | 0.007719 | 0.007836 | 0.005729 | 0.002085 |
Nuclear Periphery | 0.000496 | 0.000886 | 0.001178 | 0.000437 | 0.001046 | 0.001135 | 0.001214 | 0.000404 | 0.000761 | 0.001035 | 0.001199 | 0.000432 |
Nuclear Periphery Foci | 0.002687 | 0.007696 | 0.006997 | 0.002329 | 0.007442 | 0.002484 | 0.003298 | 0.001581 | 0.004979 | 0.004591 | 0.004798 | 0.002216 |
Nucleolus | 0.170622 | 0.141043 | 0.060811 | 0.001629 | 0.164203 | 0.121488 | 0.036903 | 0.004694 | 0.167528 | 0.129393 | 0.046597 | 0.002091 |
Nucleus | 0.568388* | 0.543164* | 0.508051* | 0.036595 | 0.585233* | 0.625279* | 0.370821* | 0.029609 | 0.576507* | 0.592083* | 0.426465* | 0.035541 |
Peroxisomes | 0.001057 | 0.002370 | 0.002769 | 0.001509 | 0.001453 | 0.001391 | 0.001111 | 0.001573 | 0.001248 | 0.001787 | 0.001783 | 0.001519 |
Vacuole | 0.010775 | 0.015222 | 0.049860 | 0.088185 | 0.008908 | 0.012488 | 0.077894 | 0.057848 | 0.009875 | 0.013593 | 0.066527 | 0.083610 |
Vacuole Periphery | 0.002383 | 0.004520 | 0.001296 | 0.000886 | 0.002527 | 0.001793 | 0.004324 | 0.000439 | 0.002452 | 0.002895 | 0.003096 | 0.000818 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -5.15 | -9.98 | -5.03 | 1.94 | 4.00 | -3.20 | -8.39 | -3.11 | -2.40 | -1.13 | -6.27 | -13.16 | -5.44 | -0.25 | 2.76 |
Bud Neck | -1.80 | -7.60 | 1.26 | 2.58 | 7.72 | -2.39 | -1.95 | 0.74 | 1.68 | 1.63 | -3.70 | -6.97 | 2.85 | 5.27 | 7.84 |
Bud Site | -2.23 | -3.26 | -3.91 | -2.16 | -0.90 | 1.16 | -1.50 | -2.80 | -3.24 | -2.15 | -0.44 | -3.25 | -4.74 | -4.45 | -2.55 |
Cell Periphery | 4.71 | 5.67 | 6.88 | 4.06 | 2.87 | 3.09 | 4.35 | 1.21 | -0.19 | -0.73 | 5.80 | 7.03 | 7.54 | 3.25 | 1.50 |
Cytoplasm | -1.19 | 0.44 | -9.74 | -8.66 | -9.08 | 0.28 | -5.72 | -5.61 | -5.66 | -3.98 | -0.03 | -3.99 | -12.05 | -11.94 | -9.25 |
Cytoplasmic Foci | -1.77 | -2.32 | -3.61 | -1.87 | -1.12 | 3.70 | -1.68 | -2.36 | -3.29 | -1.70 | 1.54 | -2.75 | -4.34 | -5.16 | -2.04 |
Eisosomes | 3.25 | 1.32 | 9.23 | 8.03 | 8.22 | -0.10 | 2.82 | 0.60 | 0.63 | -0.32 | 2.57 | 3.14 | 9.66 | 8.32 | 7.28 |
Endoplasmic Reticulum | 0.73 | 3.91 | 4.46 | 4.43 | 1.06 | -0.63 | 0.40 | 2.28 | 2.74 | 1.87 | 0.56 | 3.12 | 5.58 | 6.29 | 3.15 |
Endosome | -1.70 | 0.80 | 1.50 | 2.69 | 0.64 | 1.39 | 2.21 | 2.41 | 1.33 | 0.79 | -0.08 | 2.28 | 2.00 | 1.91 | 0.27 |
Golgi | -3.35 | -0.83 | 0.94 | 3.82 | 2.29 | -0.37 | -0.82 | 0.55 | 0.65 | 1.01 | -2.84 | -1.03 | 1.32 | 3.53 | 1.69 |
Lipid Particles | -1.53 | -4.90 | -5.55 | -5.17 | -3.56 | -0.35 | -3.15 | -2.78 | -2.63 | -1.95 | -1.03 | -5.81 | -5.93 | -5.63 | -4.44 |
Mitochondria | -1.81 | -2.42 | 0.49 | 2.83 | 5.65 | -1.82 | -2.58 | -0.26 | 1.53 | 2.25 | -2.61 | -3.56 | 0.67 | 3.90 | 5.86 |
Mitotic Spindle | -5.11 | -6.58 | -15.34 | -14.23 | -11.09 | -3.46 | -9.43 | -4.76 | -4.41 | -2.19 | -6.29 | -11.62 | -15.75 | -14.70 | -10.05 |
None | 0.91 | 6.35 | 3.29 | 2.69 | -1.05 | -0.28 | 0.99 | 1.01 | 1.18 | 0.51 | 0.07 | 3.61 | 3.39 | 3.12 | 0.91 |
Nuclear Periphery | -4.11 | -8.03 | -9.12 | -6.68 | -2.97 | -0.65 | -5.41 | -2.50 | -2.35 | -0.77 | -3.39 | -9.69 | -8.44 | -7.01 | -2.96 |
Nuclear Periphery Foci | -3.30 | -0.93 | 0.12 | 3.42 | 1.00 | 3.75 | 3.66 | 1.50 | -0.68 | -0.67 | 0.14 | 1.76 | 2.74 | 2.28 | 0.44 |
Nucleolus | 3.90 | 11.46 | 28.07* | 20.57 | 8.33 | 6.68 | 15.30 | 11.34 | 7.63 | 1.80 | 7.83 | 19.30 | 34.15* | 25.49 | 9.53 |
Nucleus | 2.72 | 2.00 | 26.06 | 21.78 | 17.28 | -3.34 | 7.22 | 11.13 | 12.22 | 7.29 | -1.14 | 6.87 | 29.71 | 29.62 | 19.40 |
Peroxisomes | -6.04 | -6.42 | -0.65 | 4.86 | 5.69 | 0.20 | 0.22 | -1.05 | -1.11 | -1.12 | -4.54 | -5.54 | -0.15 | 3.08 | 4.23 |
Vacuole | -2.61 | -5.71 | -10.89 | -9.96 | -5.56 | -2.19 | -8.51 | -3.92 | -3.61 | 0.03 | -3.19 | -10.18 | -11.65 | -10.91 | -4.56 |
Vacuole Periphery | -2.75 | 2.47 | 5.14 | 5.73 | 3.91 | 2.28 | 0.67 | 6.50 | 5.69 | 1.65 | -1.13 | 1.34 | 8.12 | 7.63 | 2.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|