Standard name
Human Ortholog
Description Essential helicase component of heterohexameric MCM2-7 complexes; MCM2-7 complexes bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.06 0 0.05 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.26 0.17 0.3 0.25 0.37 0.31 0.28 0.19 0.18 – 0.15 0.1 0.09 0.16 0.22 0.39 0.34 0.35 0.13 0.17 0.26 0.11 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0 0 0 0.08 0 0 0 0 – 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0.09
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.08 0.14 0.08 0.26 0.23 0.18 0.3 – 0.34 0.41 0 0 0.08 0 0 0 0 0 0 0 0 0
Nucleus 0.51 0.68 0.56 0.57 0.34 0.27 0.35 0.42 0.37 – 0.4 0.36 0.77 0.6 0.4 0.42 0.47 0.35 0.4 0.62 0.51 0.58 0.5 0.3
Nuclear Periphery 0 0 0 0 0 0 0 0.05 0.06 – 0.06 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.24 0.07 0.12 0.13 0.28 0.24 0.24 0.28 – 0.27 0.35 0.11 0.2 0.24 0.08 0.05 0.11 0.25 0.06 0.09 0.15 0.23 0.29
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0.08 0 0.05 0.05 0.1 0 0.06 0.05 0.05 – 0.07 0.07 0 0.07 0.08 0.16 0.13 0.17 0 0 0 0 0.12 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 0 – 1 1 0 0 1 1 0 1 0 0 0 0 0 0
Bud 1 1 5 4 4 7 7 12 13 – 11 13 0 0 1 0 1 0 13 4 5 7 7 2
Bud Neck 0 0 0 0 0 0 0 3 2 – 1 3 0 0 1 0 0 0 0 0 0 2 0 0
Bud Site 0 0 0 0 0 4 5 6 4 – 3 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 0 2 0 1 4 2 – 1 5 0 0 1 0 2 4 0 0 0 0 0 0
Cytoplasm 34 10 36 74 152 107 157 107 60 – 57 45 20 60 75 147 75 111 18 14 34 18 4 0
Endoplasmic Reticulum 1 0 0 0 2 0 1 3 2 – 1 1 0 0 2 10 6 4 0 0 1 1 1 3
Endosome 9 1 3 5 33 11 16 19 5 – 2 4 12 16 14 7 5 16 4 2 2 1 13 31
Golgi 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 4 2 0 0 0 0 1 2
Mitochondria 2 4 10 42 33 91 128 99 103 – 130 193 2 5 27 6 6 6 0 0 1 0 2 4
Nucleus 66 40 68 169 140 94 198 234 126 – 151 171 180 220 133 158 104 110 57 52 68 95 154 105
Nuclear Periphery 1 0 1 2 13 15 25 29 21 – 21 11 5 13 19 8 1 10 1 0 0 0 1 7
Nucleolus 16 14 8 36 55 95 132 135 96 – 101 166 25 72 80 31 11 36 35 4 11 25 70 103
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 5 5 1 3 3 – 2 2 0 0 0 0 0 6 6 0 2 1 9 21
Vac/Vac Membrane 10 1 6 15 42 11 31 27 17 – 28 31 8 27 28 61 29 53 3 1 3 6 38 60
Unique Cell Count 130 59 122 297 411 344 560 560 342 379 471 234 369 334 380 219 317 144 85 133 165 311 350
Labelled Cell Count 143 71 137 349 482 440 702 682 454 510 662 252 413 382 429 244 359 144 85 133 165 311 350


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.8 9.1 7.0 7.3 7.3 6.9 6.9 7.3 6.7 – 6.5 6.3 11.0 10.5 9.0 11.4 13.5 13.4 9.9 9.9 11.0
Std Deviation (1e-4) 2.1 2.1 1.4 1.9 1.7 1.7 1.6 2.2 2.3 – 1.7 1.6 2.7 2.1 2.0 3.2 3.9 4.2 2.1 2.3 2.2
Intensity Change (Log2) – – – 0.05 0.06 -0.04 -0.03 0.05 -0.08 – -0.11 -0.15 0.64 0.57 0.36 0.7 0.94 0.93 0.5 0.48 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -1.0 1.5 0.3 -0.3 -2.6 -2.8 – -3.6 -5.7 -5.2 -3.2 -1.6 1.8 0.9 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 2.2 0 0 0 0 – 0 0 1.2 0 0 0 0 1.2
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1.7 -0.1 4.2 3.7 2.6 4.8 – 5.6 6.8 0 0 0 0 -2.3 0
Nucleus 0.2 -4.3 -5.7 -4.2 -2.8 -3.6 – -3.1 -3.9 4.1 0.8 -3.0 -2.7 -1.5 -4.0
Nuclear Periphery 0 0 0 0 2.1 2.4 – 2.2 0 0 0 2.2 0 0 0
Nucleolus 1.7 2.1 4.8 4.2 4.3 4.9 – 4.7 6.2 1.3 3.4 4.2 0.6 -0.6 1.5
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0.1 1.8 0 0.3 0 0 – 0.9 0.7 0 0.9 1.2 3.1 2.4 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 6.9191 7.4645 6.282 5.7585 6.0864 6.8658 8.8444 9.2233 7.6604 6.9333 7.4017 8.6862 5.7557 5.7759 4.6177 4.4691 5.1086 5.3642
Actin 0.0232 0.0011 0.007 0.0083 0.0378 0.0004 0.0005 0.001 0.0139 0.0155 0.0021 0.0009 0.0191 0.0017 0.0083 0.0154 0.0035 0.0008
Bud 0.0006 0.0209 0.0645 0.0315 0.0075 0.0106 0.0007 0.0228 0.0788 0.0195 0.0394 0.0033 0.0005 0.0079 0.0597 0.1215 0.0417 0.0031
Bud Neck 0.0015 0.0024 0.0054 0.0072 0.0365 0.0084 0.0002 0.0014 0.0047 0.0115 0.0031 0.005 0.0011 0.0021 0.0055 0.0056 0.0033 0.0053
Bud Periphery 0.0008 0.0112 0.0452 0.0304 0.007 0.0165 0.0015 0.0118 0.0511 0.0086 0.0217 0.0023 0.001 0.0054 0.0512 0.0987 0.0417 0.004
Bud Site 0.0017 0.0551 0.0392 0.0348 0.0103 0.003 0.0006 0.0326 0.0322 0.0073 0.0053 0.001 0.0016 0.0154 0.0318 0.0247 0.0094 0.0028
Cell Periphery 0.0003 0.0024 0.0033 0.0047 0.002 0.0011 0.0001 0.0014 0.0022 0.0009 0.0004 0.0003 0.0004 0.0012 0.0032 0.0026 0.0011 0.0004
Cytoplasm 0.017 0.1103 0.3355 0.5402 0.5202 0.0028 0.0083 0.0936 0.3095 0.5655 0.6077 0.066 0.0038 0.1151 0.3599 0.477 0.6716 0.0622
Cytoplasmic Foci 0.0262 0.0046 0.0244 0.0311 0.0286 0.0001 0.0033 0.005 0.0265 0.0551 0.0142 0.0015 0.0039 0.0035 0.0329 0.0236 0.022 0.0006
Eisosomes 0.0003 0 0.0002 0.0003 0.0003 0 0 0 0 0.0001 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0055 0.0106 0.0047 0.0067 0.0066 0.0001 0.0013 0.0018 0.0047 0.0058 0.0167 0.0014 0.001 0.0024 0.0122 0.0077 0.004 0.0008
Endosome 0.0425 0.0088 0.0152 0.0358 0.0529 0.0002 0.0092 0.0036 0.0215 0.0613 0.0145 0.0071 0.0066 0.003 0.0234 0.0237 0.023 0.0018
Golgi 0.0067 0.0013 0.0022 0.0026 0.0124 0.0001 0.0004 0.0003 0.0053 0.0127 0.0011 0.002 0.0012 0.0004 0.0024 0.0014 0.0011 0.0001
Lipid Particles 0.0132 0.0008 0.0092 0.0132 0.0331 0.0001 0.003 0.0007 0.0039 0.03 0.0007 0.0011 0.0033 0.0007 0.0088 0.0012 0.0004 0.0001
Mitochondria 0.0045 0.0011 0.0129 0.0283 0.011 0.0008 0.0017 0.0021 0.008 0.004 0.0012 0.0071 0.0029 0.0013 0.0042 0.0036 0.0016 0.0009
None 0.0037 0.0009 0.0063 0.0052 0.0031 0.0001 0.0029 0.0012 0.0029 0.0033 0.0021 0.0008 0.0012 0.0014 0.0049 0.0055 0.0046 0.0003
Nuclear Periphery 0.0143 0.0064 0.0222 0.0074 0.0182 0.0017 0.0298 0.0065 0.022 0.0099 0.0086 0.0052 0.0082 0.0365 0.0185 0.0117 0.0058 0.0036
Nucleolus 0.0818 0.0974 0.0449 0.0479 0.035 0.2296 0.0313 0.04 0.0238 0.0084 0.0213 0.1092 0.0384 0.0426 0.0318 0.0105 0.0244 0.1393
Nucleus 0.7203 0.6431 0.2798 0.0771 0.0406 0.7132 0.8965 0.7228 0.3198 0.108 0.1729 0.779 0.8883 0.7024 0.2412 0.1042 0.0894 0.7653
Peroxisomes 0.0035 0.0002 0.0102 0.0024 0.0232 0.0001 0.0001 0.0003 0.0049 0.002 0.0002 0.0002 0.0044 0.0002 0.0173 0.0013 0.0005 0.0001
Punctate Nuclear 0.0228 0.0042 0.02 0.0061 0.0126 0.0062 0.0036 0.0147 0.0185 0.0205 0.0518 0.0017 0.0096 0.0465 0.0412 0.0066 0.0029 0.005
Vacuole 0.0073 0.0163 0.0421 0.0729 0.092 0.0042 0.0033 0.0306 0.0387 0.042 0.0133 0.0038 0.0028 0.0087 0.0363 0.0468 0.0429 0.0029
Vacuole Periphery 0.0021 0.0011 0.0056 0.0061 0.009 0.0005 0.0015 0.0058 0.007 0.0081 0.0017 0.0013 0.0007 0.0016 0.0054 0.0065 0.0052 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.5703 12.6729 28.4728 43.2682 22.465 24.7951 18.0636 30.6294 37.2433 30.3152
Translational Efficiency 0.7872 0.613 1.1431 0.7926 0.7621 0.676 0.8404 0.7217 0.7997 0.7421

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1741 1081 444 428 1620 1593 651 76 3361 2674 1095 504

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 919.39 1044.59 1544.27 1459.19 977.04 1033.46 1351.83 1503.73 947.18 1037.96 1429.86 1465.91
Standard Deviation 135.06 206.20 220.46 226.82 183.77 192.84 233.99 229.25 162.96 198.42 247.36 227.75
Intensity Change Log 2 — 0.184188 0.748176 0.666419 — 0.080993 0.468424 0.622056 — 0.131944 0.610825 0.643734

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000567 0.001430 0.001309 0.000563 0.000706 0.000906 0.001011 0.001224 0.000634 0.001118 0.001132 0.000662
Bud Neck 0.029198 0.030626 0.037813 0.005611 0.039502 0.043580 0.025556 0.008300 0.034165 0.038343 0.030526 0.006016
Bud Site 0.017529 0.019588 0.017730 0.034203 0.016699 0.014096 0.019515 0.027144 0.017129 0.016317 0.018791 0.033139
Cell Periphery 0.001603 0.000721 0.000524 0.000514 0.000769 0.000545 0.000444 0.000489 0.001201 0.000616 0.000477 0.000510
Cytoplasm 0.149865* 0.165863* 0.203528* 0.616577* 0.118924* 0.115948* 0.293818* 0.691709* 0.134951* 0.136127* 0.257207* 0.627906*
Cytoplasmic Foci 0.017264 0.022944 0.035042 0.053912 0.017348 0.010757 0.045299 0.065444 0.017304 0.015684 0.041140 0.055651
Eisosomes 0.000059 0.000045 0.000044 0.000008 0.000048 0.000047 0.000030 0.000020 0.000053 0.000046 0.000036 0.000009
Endoplasmic Reticulum 0.003707 0.003249 0.001584 0.000284 0.002884 0.002959 0.002592 0.000335 0.003310 0.003076 0.002183 0.000291
Endosome 0.005303 0.006944 0.007151 0.006852 0.005047 0.004444 0.007272 0.004378 0.005179 0.005455 0.007223 0.006479
Golgi 0.001182 0.003495 0.001619 0.001265 0.001148 0.001335 0.003363 0.000841 0.001166 0.002208 0.002656 0.001201
Lipid Particles 0.000668 0.000878 0.002894 0.007068 0.000678 0.000834 0.001688 0.004441 0.000673 0.000851 0.002177 0.006672
Mitochondria 0.002007 0.003013 0.002199 0.000247 0.002187 0.003365 0.003737 0.000314 0.002093 0.003223 0.003113 0.000257
Mitotic Spindle 0.006928 0.019258 0.054534 0.139267* 0.015523 0.026752 0.092568 0.096994 0.011071 0.023723 0.077146 0.132893*
None 0.007713 0.007044 0.003069 0.002062 0.007726 0.008373 0.007544 0.002219 0.007719 0.007836 0.005729 0.002085
Nuclear Periphery 0.000496 0.000886 0.001178 0.000437 0.001046 0.001135 0.001214 0.000404 0.000761 0.001035 0.001199 0.000432
Nuclear Periphery Foci 0.002687 0.007696 0.006997 0.002329 0.007442 0.002484 0.003298 0.001581 0.004979 0.004591 0.004798 0.002216
Nucleolus 0.170622 0.141043 0.060811 0.001629 0.164203 0.121488 0.036903 0.004694 0.167528 0.129393 0.046597 0.002091
Nucleus 0.568388* 0.543164* 0.508051* 0.036595 0.585233* 0.625279* 0.370821* 0.029609 0.576507* 0.592083* 0.426465* 0.035541
Peroxisomes 0.001057 0.002370 0.002769 0.001509 0.001453 0.001391 0.001111 0.001573 0.001248 0.001787 0.001783 0.001519
Vacuole 0.010775 0.015222 0.049860 0.088185 0.008908 0.012488 0.077894 0.057848 0.009875 0.013593 0.066527 0.083610
Vacuole Periphery 0.002383 0.004520 0.001296 0.000886 0.002527 0.001793 0.004324 0.000439 0.002452 0.002895 0.003096 0.000818

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.15 -9.98 -5.03 1.94 4.00 -3.20 -8.39 -3.11 -2.40 -1.13 -6.27 -13.16 -5.44 -0.25 2.76
Bud Neck -1.80 -7.60 1.26 2.58 7.72 -2.39 -1.95 0.74 1.68 1.63 -3.70 -6.97 2.85 5.27 7.84
Bud Site -2.23 -3.26 -3.91 -2.16 -0.90 1.16 -1.50 -2.80 -3.24 -2.15 -0.44 -3.25 -4.74 -4.45 -2.55
Cell Periphery 4.71 5.67 6.88 4.06 2.87 3.09 4.35 1.21 -0.19 -0.73 5.80 7.03 7.54 3.25 1.50
Cytoplasm -1.19 0.44 -9.74 -8.66 -9.08 0.28 -5.72 -5.61 -5.66 -3.98 -0.03 -3.99 -12.05 -11.94 -9.25
Cytoplasmic Foci -1.77 -2.32 -3.61 -1.87 -1.12 3.70 -1.68 -2.36 -3.29 -1.70 1.54 -2.75 -4.34 -5.16 -2.04
Eisosomes 3.25 1.32 9.23 8.03 8.22 -0.10 2.82 0.60 0.63 -0.32 2.57 3.14 9.66 8.32 7.28
Endoplasmic Reticulum 0.73 3.91 4.46 4.43 1.06 -0.63 0.40 2.28 2.74 1.87 0.56 3.12 5.58 6.29 3.15
Endosome -1.70 0.80 1.50 2.69 0.64 1.39 2.21 2.41 1.33 0.79 -0.08 2.28 2.00 1.91 0.27
Golgi -3.35 -0.83 0.94 3.82 2.29 -0.37 -0.82 0.55 0.65 1.01 -2.84 -1.03 1.32 3.53 1.69
Lipid Particles -1.53 -4.90 -5.55 -5.17 -3.56 -0.35 -3.15 -2.78 -2.63 -1.95 -1.03 -5.81 -5.93 -5.63 -4.44
Mitochondria -1.81 -2.42 0.49 2.83 5.65 -1.82 -2.58 -0.26 1.53 2.25 -2.61 -3.56 0.67 3.90 5.86
Mitotic Spindle -5.11 -6.58 -15.34 -14.23 -11.09 -3.46 -9.43 -4.76 -4.41 -2.19 -6.29 -11.62 -15.75 -14.70 -10.05
None 0.91 6.35 3.29 2.69 -1.05 -0.28 0.99 1.01 1.18 0.51 0.07 3.61 3.39 3.12 0.91
Nuclear Periphery -4.11 -8.03 -9.12 -6.68 -2.97 -0.65 -5.41 -2.50 -2.35 -0.77 -3.39 -9.69 -8.44 -7.01 -2.96
Nuclear Periphery Foci -3.30 -0.93 0.12 3.42 1.00 3.75 3.66 1.50 -0.68 -0.67 0.14 1.76 2.74 2.28 0.44
Nucleolus 3.90 11.46 28.07* 20.57 8.33 6.68 15.30 11.34 7.63 1.80 7.83 19.30 34.15* 25.49 9.53
Nucleus 2.72 2.00 26.06 21.78 17.28 -3.34 7.22 11.13 12.22 7.29 -1.14 6.87 29.71 29.62 19.40
Peroxisomes -6.04 -6.42 -0.65 4.86 5.69 0.20 0.22 -1.05 -1.11 -1.12 -4.54 -5.54 -0.15 3.08 4.23
Vacuole -2.61 -5.71 -10.89 -9.96 -5.56 -2.19 -8.51 -3.92 -3.61 0.03 -3.19 -10.18 -11.65 -10.91 -4.56
Vacuole Periphery -2.75 2.47 5.14 5.73 3.91 2.28 0.67 6.50 5.69 1.65 -1.13 1.34 8.12 7.63 2.29
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Essential helicase component of heterohexameric MCM2-7 complexes; MCM2-7 complexes bind pre-replication complexes on DNA and melt DNA prior to replication; forms an Mcm4p-6p-7p subcomplex; shows nuclear accumulation in G1; homolog of S. pombe Cdc21p
Localization
Cell Percentages nucleus (84%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Mcm4

Mcm4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcm4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available