Standard name
Human Ortholog
Description Cyclin associated with protein kinase Kin28p; Kin28p is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters; human homolog CCNH allows growth of yeast ccl1 temperature-sensitive mutant at restrictive temperature
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0.1 0.13 0.14 0.09 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.09 0 0.09 0.13 0.29 0.25 0.25 0.29 0.4 0.42 0.37 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.91 0.95 0.91 0.88 0.86 0.83 0.82 0.82 0.76 0.74 0.69 0.7 0.89 0.85 0.83 0.86 0.89 0.89 0.87 0.88 0.82 0.83 0.84 0.73
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.19 0.11 0.08 0.11 0.14 0.24 0.24 0.27 0.26 0.29 0.29 0 0 0 0.05 0 0.06 0.06 0.06 0.1 0.06 0.06 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 1 0 2 1 3 3 5 3 7 4 0 0 0 0 2 1 0 3 8 1 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 1 0 0 1 3 11 13 19 16 15 9 24 0 0 1 0 1 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 9 16 12 9 10 10 5 6 7 3 45 56 64 28 16 14 0 0 1 1 1 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 2 2 0 0 0 0 0 0
Endosome 0 0 0 1 1 1 0 0 0 0 0 0 1 0 3 0 3 2 2 0 1 4 3 5
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 1 1 3 2 0 1
Mitochondria 41 16 14 28 44 161 118 118 147 188 222 255 2 0 4 11 5 6 2 0 0 1 1 8
Nucleus 207 178 348 276 298 459 388 388 378 351 368 482 412 359 380 281 255 232 205 174 321 294 285 299
Nuclear Periphery 0 0 5 1 0 5 9 8 5 4 5 11 2 2 3 2 6 1 0 0 1 3 0 2
Nucleolus 49 35 44 24 38 79 112 112 135 125 152 197 16 5 13 15 11 15 14 11 38 21 21 41
Peroxisomes 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 0 1 1 0 1 1 0 6 3 8 0 6 1 1 0 2 2 3 3
Vac/Vac Membrane 1 1 3 1 0 1 3 2 3 2 3 3 4 2 6 7 5 6 4 4 11 15 18 34
Unique Cell Count 228 188 383 315 345 550 476 476 500 474 533 689 465 424 458 326 288 262 235 198 392 354 340 410
Labelled Cell Count 302 233 429 351 399 727 657 661 694 695 775 981 490 427 483 352 313 280 235 198 392 354 340 410


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 7.0 6.2 5.8 5.6 4.9 4.9 4.9 4.9 4.7 4.2 4.6 7.6 7.2 6.8 8.8 8.6 8.3 7.6 7.5 8.1
Std Deviation (1e-4) 1.2 1.2 1.4 1.7 1.6 1.3 1.2 1.2 1.2 1.1 1.3 1.1 1.6 1.6 1.6 2.4 1.9 2.0 1.3 1.5 1.4
Intensity Change (Log2) – – – -0.09 -0.15 -0.35 -0.35 -0.35 -0.34 -0.41 -0.55 -0.44 0.3 0.22 0.13 0.51 0.47 0.42 0.29 0.27 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.5 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 0.9 -0.8 -0.2 -0.2 -1.6 -1.2 -1.2 0 4.3 5.7 6.0 3.7 2.2 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.9 4.5 0 0 0 9.8 0 0 0 -3.4 -4.0 -2.8 -0.2 -1.5 -1.0
Nucleus -1.4 -1.9 -3.3 -3.9 -3.9 -5.9 -6.3 -7.9 -7.8 -1.1 -2.7 -3.3 -2.0 -1.0 -1.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.7 -0.2 0 0 0 5.7 0 0 0 -4.5 -6.1 -5.0 -3.3 -3.6 -2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9709 3.6593 2.9417 3.2124 2.5899 3.2329 3.5041 3.4695 3.1018 3.3463 2.839 3.2178 3.0083 3.1163 2.6797 3.0998 2.8754 2.6616
Actin 0.0284 0 0.0003 0.0001 0 0.0009 0.0162 0.0071 0.0146 0.0118 0.0011 0.0036 0.0123 0 0.0184 0.0008 0.0366 0
Bud 0.0002 0 0 0 0 0 0.0005 0 0.0003 0.0004 0.0002 0.0001 0.0007 0 0.0004 0.0001 0.0011 0
Bud Neck 0.0005 0 0 0.0002 0.0002 0.0007 0.0014 0.0001 0.0032 0.0006 0.0008 0.0007 0.0012 0 0.0012 0.0003 0.0032 0.0007
Bud Periphery 0.0003 0 0 0 0 0.0001 0.0018 0 0.0008 0.0006 0.0003 0.0002 0.0021 0 0.001 0 0.0037 0
Bud Site 0.0006 0 0 0 0 0.0001 0.0019 0.0003 0.0009 0.0017 0.0006 0.0002 0.0011 0 0.0016 0.0003 0.004 0
Cell Periphery 0.0001 0 0 0 0 0 0.0005 0 0.0002 0.0001 0.0001 0 0.0004 0 0.0003 0.0001 0.0007 0
Cytoplasm 0.005 0 0 0.0004 0 0.003 0.0019 0 0.0009 0.0027 0.0014 0.0007 0.0057 0 0.0006 0.0207 0.0011 0.0001
Cytoplasmic Foci 0.0073 0 0.0004 0.0016 0 0.0009 0.0071 0.0001 0.0064 0.0029 0.0063 0.0005 0.0059 0 0.0013 0.0009 0.0164 0
Eisosomes 0.0006 0 0 0 0 0 0.0004 0 0.0002 0.0001 0 0 0.0001 0 0.0001 0 0.0007 0
Endoplasmic Reticulum 0.0029 0 0 0.0002 0 0.0002 0.0015 0 0.0006 0.003 0.0009 0.0014 0.002 0 0.0011 0.0051 0.0029 0
Endosome 0.0097 0 0.0003 0.0017 0 0.002 0.0085 0 0.0032 0.0057 0.0081 0.0026 0.0102 0 0.0016 0.0017 0.0217 0.0001
Golgi 0.0035 0 0.0006 0.0002 0 0.0009 0.003 0.0001 0.0016 0.0012 0.0014 0.0011 0.0037 0 0.0011 0.0001 0.0074 0
Lipid Particles 0.0089 0 0.0043 0.001 0 0.0024 0.0136 0 0.0123 0.0011 0.0188 0.0019 0.0027 0 0.0071 0.0003 0.0203 0
Mitochondria 0.0028 0 0.0007 0.0001 0 0.0006 0.0208 0.0001 0.0079 0.0011 0.0037 0.0013 0.008 0.0001 0.0086 0.0003 0.0387 0.0002
None 0.0051 0 0 0.0003 0 0.0001 0.0104 0 0.0006 0.0015 0.001 0.0003 0.0032 0 0.0002 0.0009 0.0016 0
Nuclear Periphery 0.0108 0.0003 0.0003 0.0212 0.0005 0.0016 0.0266 0.0005 0.0046 0.0161 0.0092 0.0111 0.0068 0.0005 0.003 0.0091 0.0292 0.0013
Nucleolus 0.0198 0.0118 0.0077 0.0118 0.085 0.0605 0.0228 0.0125 0.0168 0.0068 0.0642 0.0399 0.0158 0.0085 0.0141 0.0054 0.0933 0.0348
Nucleus 0.8698 0.9877 0.9818 0.9039 0.9097 0.9203 0.834 0.978 0.9101 0.9325 0.8617 0.9271 0.908 0.9908 0.9341 0.9488 0.6927 0.961
Peroxisomes 0.0149 0 0.0031 0.0001 0 0.0004 0.0056 0.0001 0.0093 0.0004 0.0031 0.0004 0.0005 0 0.0011 0 0.0032 0
Punctate Nuclear 0.007 0.0002 0.0002 0.0567 0.0045 0.0049 0.0143 0.0009 0.0029 0.0087 0.0149 0.0062 0.0034 0.0001 0.0013 0.0041 0.0049 0.0014
Vacuole 0.0009 0 0 0.0002 0 0.0002 0.0038 0 0.002 0.0006 0.0012 0.0004 0.0042 0 0.0011 0.0007 0.0081 0.0001
Vacuole Periphery 0.0007 0 0 0.0003 0 0.0001 0.0033 0 0.0008 0.0006 0.0009 0.0004 0.0019 0 0.0007 0.0002 0.0088 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.2699 10.8485 9.9077 17.067 16.9446 3.732 17.719 19.0776 19.6195 17.2712
Translational Efficiency 1.1068 1.8609 1.6265 1.1088 0.9317 4.5723 1.0381 1.0523 0.9965 1.1392

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1820 409 2951 1375 1665 512 176 950 3485 921 3127 2325

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 827.84 975.71 1033.64 992.93 778.92 908.26 1045.09 1126.37 804.47 938.21 1034.28 1047.45
Standard Deviation 107.59 137.41 142.16 160.14 114.18 134.78 136.89 172.69 113.45 140.02 141.89 177.92
Intensity Change Log 2 — 0.237100 0.320310 0.262340 — 0.221630 0.424080 0.532134 — 0.229622 0.371548 0.399433

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000437 0.000518 0.000550 0.001063 0.000397 0.000489 0.001064 0.001094 0.000418 0.000502 0.000579 0.001076
Bud Neck 0.005701 0.008128 0.031456 0.024467 0.004827 0.008466 0.006990 0.025027 0.005283 0.008316 0.030079 0.024696
Bud Site 0.000414 0.000491 0.001168 0.002884 0.000763 0.000624 0.000689 0.004864 0.000580 0.000565 0.001141 0.003693
Cell Periphery 0.000038 0.000020 0.000045 0.000144 0.000158 0.000084 0.000025 0.000111 0.000096 0.000055 0.000044 0.000131
Cytoplasm 0.000441 0.000100 0.000196 0.001993 0.001538 0.001508 0.000125 0.001882 0.000965 0.000883 0.000192 0.001948
Cytoplasmic Foci 0.000319 0.001372 0.000004 0.001456 0.000129 0.000671 0.000019 0.001397 0.000228 0.000982 0.000005 0.001432
Eisosomes 0.000021 0.000029 0.000043 0.000037 0.000021 0.000027 0.000043 0.000033 0.000021 0.000028 0.000043 0.000035
Endoplasmic Reticulum 0.002661 0.002442 0.002172 0.003550 0.003057 0.003166 0.003187 0.003130 0.002850 0.002845 0.002229 0.003379
Endosome 0.000200 0.000618 0.000076 0.002276 0.000277 0.000134 0.000061 0.002668 0.000237 0.000349 0.000075 0.002437
Golgi 0.000174 0.000477 0.000069 0.001229 0.000115 0.000102 0.000104 0.004467 0.000145 0.000269 0.000071 0.002552
Lipid Particles 0.000342 0.000124 0.000015 0.000510 0.000130 0.000355 0.000053 0.000147 0.000241 0.000253 0.000018 0.000361
Mitochondria 0.001617 0.002222 0.001282 0.003854 0.001065 0.000780 0.001068 0.007885 0.001353 0.001420 0.001270 0.005501
Mitotic Spindle 0.002901 0.003221 0.000692 0.014301 0.001987 0.006027 0.000323 0.016418 0.002464 0.004781 0.000671 0.015166
None 0.001591 0.000927 0.000846 0.004478 0.003498 0.002596 0.001053 0.002205 0.002502 0.001855 0.000858 0.003549
Nuclear Periphery 0.000474 0.000084 0.000222 0.001684 0.000275 0.000135 0.000127 0.002204 0.000379 0.000112 0.000217 0.001896
Nuclear Periphery Foci 0.000718 0.000612 0.000023 0.000821 0.000710 0.000198 0.000071 0.001450 0.000714 0.000382 0.000026 0.001078
Nucleolus 0.042515 0.033557 0.023550 0.024238 0.037750 0.031004 0.036167 0.016441 0.040238 0.032138 0.024261 0.021053
Nucleus 0.938223* 0.943516* 0.937050* 0.901618* 0.942331* 0.942783* 0.947128* 0.894149* 0.940186* 0.943109* 0.937617* 0.898566*
Peroxisomes 0.000424 0.000698 0.000181 0.000572 0.000373 0.000423 0.001069 0.000415 0.000399 0.000545 0.000231 0.000508
Vacuole 0.000359 0.000308 0.000244 0.004475 0.000363 0.000319 0.000523 0.008118 0.000361 0.000314 0.000259 0.005964
Vacuole Periphery 0.000430 0.000537 0.000115 0.004349 0.000236 0.000109 0.000111 0.005895 0.000337 0.000299 0.000115 0.004981

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.49 -1.43 -4.63 -12.12 -10.89 -1.82 -10.06 -9.75 -7.99 -1.83 -1.05 -3.21 -8.98 -13.14 -11.16
Bud Neck -2.73 -25.61 -15.26 -12.13 4.37 -2.96 -3.46 -13.61 -9.82 -10.22 -3.82 -26.85 -20.54 -15.02 3.32
Bud Site -0.72 -10.14 -8.47 -8.38 -5.04 0.56 -0.38 -3.96 -4.13 -3.94 0.11 -6.94 -6.92 -7.05 -5.13
Cell Periphery 3.37 -1.09 -5.22 -6.61 -4.86 0.79 1.53 0.68 -0.33 -3.32 0.88 1.24 -0.44 -2.75 -5.22
Cytoplasm 3.69 2.28 -5.80 -7.76 -7.17 -0.10 3.58 -0.29 -0.06 -3.73 0.03 3.81 -2.83 -1.49 -7.17
Cytoplasmic Foci -0.82 2.31 -1.72 0.34 -2.92 -1.13 3.86 -2.78 -0.22 -3.23 -1.25 3.13 -2.98 0.17 -4.22
Eisosomes -4.12 -18.59 -11.80 -4.84 4.30 -2.48 -8.31 -9.05 -4.27 2.24 -4.31 -20.64 -14.72 -6.30 4.99
Endoplasmic Reticulum 0.98 1.91 -3.39 -4.14 -5.56 -0.51 -0.22 -1.13 -0.39 -0.69 -0.05 3.63 -3.35 -2.82 -6.86
Endosome -0.87 3.35 -3.29 -1.47 -3.59 1.91 3.10 -3.36 -3.72 -3.89 -0.50 4.08 -4.63 -3.52 -5.19
Golgi -0.81 1.66 -2.45 -0.84 -2.85 0.29 -0.03 -2.25 -2.26 -2.25 -0.71 2.00 -2.91 -2.57 -3.03
Lipid Particles 1.44 2.31 -0.01 -0.96 -1.45 -0.96 3.48 -0.13 0.94 -3.09 -0.10 3.01 -0.12 -0.01 -1.80
Mitochondria -0.35 0.36 -3.60 -0.80 -5.70 1.23 -0.51 -4.39 -4.65 -4.35 -0.07 -0.47 -5.77 -3.71 -5.97
Mitotic Spindle -0.12 1.66 -4.37 -3.23 -5.39 -1.22 1.23 -4.25 -2.03 -4.75 -1.07 1.73 -6.14 -3.43 -7.06
None 2.91 3.10 -3.87 -5.16 -5.17 0.84 6.00 3.06 0.45 -3.96 1.14 7.32 -1.90 -2.24 -6.29
Nuclear Periphery 2.47 1.20 -3.36 -5.40 -4.64 2.90 2.74 -3.20 -3.47 -3.45 3.11 1.21 -4.70 -5.90 -5.30
Nuclear Periphery Foci 0.15 1.78 0.17 -0.05 -3.39 1.87 2.31 -0.77 -2.13 -2.41 1.09 2.93 -0.39 -1.55 -3.84
Nucleolus 2.81 6.58 7.11 2.59 1.90 1.94 -0.30 6.82 3.00 3.07 3.42 6.47 9.58 3.71 3.56
Nucleus -0.99 3.42 6.06 5.46 4.32 -0.01 0.11 6.66 5.51 4.99 -0.69 4.71 9.04 7.68 6.29
Peroxisomes -1.25 1.56 -0.37 1.17 -3.58 -0.59 -4.81 -2.03 -1.04 3.84 -1.27 1.76 -1.11 0.43 -4.67
Vacuole 0.44 0.45 -4.60 -4.69 -4.72 0.19 -1.83 -4.37 -4.38 -4.22 0.42 0.50 -6.25 -6.30 -6.33
Vacuole Periphery -0.23 1.98 -2.92 -2.57 -3.36 2.00 1.89 -3.10 -3.22 -3.21 0.23 2.48 -4.28 -4.24 -4.64
Standard name
Human Ortholog
Description Cyclin associated with protein kinase Kin28p; Kin28p is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters; human homolog CCNH allows growth of yeast ccl1 temperature-sensitive mutant at restrictive temperature
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ccl1

Ccl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ccl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available