Standard name
Human Ortholog
Description Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.11 0.12 0.05 0.07 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.09 0.11 0.09 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.87 0.83 0.86 0.85 0.74 0.61 0.67 0.57 0.64 0.44 0.46 0.83 0.74 0.73 0.79 0.78 0.7 0.76 0.73 0.6 0.58 0.62 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.37 0.49 0.63 0.47 0.34 0.56 0.54 0.56 0.75 0.65 0.73 0.72 0.39 0.3 0.31 0.22 0.21 0.43 0.19 0.2 0.36 0.37 0.29 0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 1 2 0 0 0 3
Bud Neck 0 0 0 0 0 1 0 1 0 2 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 1 1 0 7 6 6 2 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 2 2 1 6 3 4 1 6 2 2 26 42 52 17 14 7 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 3 1 0 0 0 0 0 0 0
Endosome 0 0 0 1 0 0 0 0 0 0 0 0 6 8 7 2 1 2 1 2 0 0 3 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 2 1
Mitochondria 5 3 0 2 2 6 7 23 14 18 5 12 0 0 0 3 4 2 1 3 0 0 2 0
Nucleus 323 194 65 100 132 123 88 170 75 125 38 58 315 290 312 246 161 87 295 180 55 85 222 170
Nuclear Periphery 0 0 1 0 0 0 1 1 0 2 0 1 0 0 0 0 1 0 1 0 0 0 1 0
Nucleolus 133 110 49 55 52 94 78 143 99 127 63 91 150 116 131 70 44 54 74 49 33 55 103 84
Peroxisomes 1 1 0 0 0 0 0 1 0 0 0 0 1 10 5 3 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 1 5 13 12 3 1 0 2 1 0 0 1 1
Vac/Vac Membrane 0 0 0 1 0 0 1 0 0 0 0 0 1 2 5 7 4 0 6 5 0 2 12 16
Unique Cell Count 358 224 78 116 155 167 144 255 132 194 86 127 381 390 427 313 206 125 388 248 93 148 356 285
Labelled Cell Count 465 310 117 161 188 231 179 350 195 287 111 174 504 482 525 354 232 153 388 248 93 148 356 285


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.9 17.6 13.8 12.5 13.5 11.9 11.3 11.2 10.5 10.5 10.0 9.8 16.3 15.7 15.3 18.9 21.4 19.5 14.7 14.8 16.6
Std Deviation (1e-4) 3.7 5.4 4.4 3.7 4.6 3.3 4.1 4.2 3.8 4.7 3.6 3.5 5.6 6.4 6.1 8.7 8.0 7.6 3.5 3.6 3.5
Intensity Change (Log2) – – – -0.14 -0.02 -0.21 -0.28 -0.3 -0.39 -0.4 -0.47 -0.49 0.25 0.19 0.16 0.46 0.64 0.5 0.1 0.1 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 2.3 2.5 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 2.7 3.0 2.8 0 0 0 0 0 0 0 0
Nucleus 0.6 0.4 -1.7 -3.4 -2.8 -3.9 -3.1 -5.2 -5.3 -0.1 -1.7 -1.9 -0.9 -1.0 -2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.1 -4.3 -1.0 -1.2 -1.1 1.9 0.4 1.4 1.3 -3.8 -5.6 -5.4 -6.9 -6.6 -2.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 10.0942 11.3127 10.3257 11.0619 8.6707 9.6097 16.8693 13.5509 14.6877 13.8074 13.7733 14.3419 18.0657 14.5643 16.2777 15.2149 15.5451 15.3244
Actin 0.015 0 0.0009 0 0.0079 0 0.0011 0.0002 0.0004 0 0.0221 0 0 0 0.0019 0 0 0
Bud 0.0011 0 0.0002 0 0.0006 0.0001 0 0 0 0 0.0001 0 0 0 0 0 0 0
Bud Neck 0.0015 0 0.0002 0.0001 0.0016 0.0004 0.0001 0 0.0001 0 0.0002 0.0002 0 0 0 0 0.0001 0.0002
Bud Periphery 0.0011 0 0.0003 0.0001 0.0013 0.0001 0 0.0001 0 0 0.0002 0 0.0001 0 0 0 0.0001 0
Bud Site 0.0019 0 0.0009 0.0001 0.0009 0.0001 0.0001 0 0.0003 0 0.0002 0 0.0002 0 0 0 0 0
Cell Periphery 0.0006 0 0.0001 0 0.0011 0.0001 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0027 0 0.0012 0 0.0019 0 0.0014 0.0001 0.0008 0 0.0001 0 0.0002 0 0.0001 0 0 0
Cytoplasmic Foci 0.0247 0 0.0032 0.0002 0.016 0.0043 0.0054 0 0.0019 0 0.0009 0 0.0012 0 0.0006 0 0 0
Eisosomes 0.0001 0 0 0 0.0006 0 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0017 0 0.0009 0 0.0014 0 0.0012 0 0 0 0.0003 0 0.0001 0 0.0004 0 0 0
Endosome 0.0196 0 0.0146 0.0001 0.0039 0.0001 0.0026 0 0.0006 0 0.0024 0 0.001 0 0.0012 0 0 0
Golgi 0.0081 0 0.0011 0 0.0018 0.0001 0.0004 0 0.0011 0 0.0037 0 0.0001 0 0.0003 0 0 0
Lipid Particles 0.0109 0 0.0045 0.0003 0.0122 0.003 0.002 0 0.0004 0 0.003 0 0.0014 0 0.0003 0 0 0
Mitochondria 0.0036 0.0002 0.0038 0.0007 0.005 0.0002 0.0005 0.0001 0.0009 0.0001 0.0019 0.0001 0.0004 0.0001 0.0002 0 0.0004 0.0001
None 0.0019 0 0.0008 0.0001 0.0121 0 0.0053 0 0.0002 0 0.0003 0 0.0007 0 0.0002 0 0 0
Nuclear Periphery 0.0242 0.0004 0.0056 0.0007 0.0056 0.0001 0.0064 0.0062 0.0003 0.0002 0.0013 0 0.0003 0.0005 0.001 0.0002 0.0003 0.0001
Nucleolus 0.1218 0.0563 0.0519 0.1451 0.4769 0.2962 0.0652 0.0548 0.027 0.0346 0.3161 0.1838 0.0665 0.0564 0.0392 0.0574 0.3567 0.1999
Nucleus 0.7233 0.9426 0.9048 0.8493 0.4168 0.6945 0.8925 0.9377 0.9584 0.965 0.6439 0.8154 0.9266 0.9427 0.9512 0.942 0.641 0.7993
Peroxisomes 0.0066 0 0.0007 0.0001 0.0015 0.0001 0.0011 0 0.0062 0 0.0017 0 0.0001 0 0 0 0 0
Punctate Nuclear 0.0236 0.0001 0.0017 0.0025 0.0284 0.0005 0.0138 0.0002 0.001 0.0001 0.001 0.0001 0.0006 0.0002 0.0033 0.0003 0.0012 0.0002
Vacuole 0.0033 0.0001 0.0013 0.0002 0.0013 0.0001 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0001 0 0 0.0001 0.0001
Vacuole Periphery 0.0028 0.0001 0.0013 0.0003 0.0011 0.0002 0.0002 0.0004 0 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.473 42.6467 31.1353 27.7899 28.9315 38.4517 36.5129 30.5113 33.0784 39.1083
Translational Efficiency 1.0607 1.0444 0.8411 1.0903 1.2617 0.8641 1.1814 0.9556 0.9871 0.9388

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1871 1603 1750 1100 608 1383 1808 85 2479 2986 3558 1185

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1166.54 1294.30 1475.76 1218.72 1314.21 1238.72 1484.27 1632.49 1202.76 1268.56 1480.08 1248.40
Standard Deviation 211.88 336.75 317.13 325.38 243.20 295.97 283.47 322.17 228.97 319.72 300.53 342.23
Intensity Change Log 2 — 0.149936 0.339222 0.063131 — -0.085346 0.175558 0.312878 — 0.030082 0.254835 0.200798

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000517 0.000563 0.000753 0.001031 0.000512 0.000680 0.000636 0.001096 0.000516 0.000617 0.000694 0.001036
Bud Neck 0.005897 0.002914 0.011949 0.012165 0.002193 0.005284 0.010126 0.012884 0.004989 0.004012 0.011023 0.012216
Bud Site 0.001030 0.000607 0.000965 0.003518 0.000276 0.001233 0.000886 0.001776 0.000845 0.000897 0.000925 0.003393
Cell Periphery 0.000141 0.000083 0.000037 0.000279 0.000009 0.000092 0.000067 0.000052 0.000108 0.000087 0.000052 0.000262
Cytoplasm 0.000158 0.000647 0.000081 0.010946 0.000005 0.000361 0.000122 0.000194 0.000121 0.000514 0.000102 0.010175
Cytoplasmic Foci 0.000290 0.000213 0.000094 0.001523 0.000065 0.001967 0.000151 0.002112 0.000235 0.001025 0.000123 0.001565
Eisosomes 0.000263 0.000181 0.000122 0.000075 0.000178 0.000210 0.000121 0.000087 0.000242 0.000195 0.000121 0.000076
Endoplasmic Reticulum 0.001018 0.000421 0.000473 0.000982 0.000421 0.000676 0.000367 0.000437 0.000872 0.000539 0.000419 0.000943
Endosome 0.000087 0.000062 0.000020 0.000578 0.000007 0.000216 0.000051 0.008308 0.000068 0.000133 0.000036 0.001133
Golgi 0.000052 0.000092 0.000072 0.000252 0.000040 0.000299 0.000059 0.003177 0.000049 0.000188 0.000065 0.000461
Lipid Particles 0.000022 0.000014 0.000007 0.000084 0.000004 0.000701 0.000022 0.000030 0.000018 0.000333 0.000014 0.000080
Mitochondria 0.001057 0.000967 0.001673 0.002085 0.000889 0.001380 0.001341 0.003468 0.001016 0.001158 0.001504 0.002185
Mitotic Spindle 0.000342 0.000043 0.000723 0.009650 0.000026 0.001858 0.000204 0.001718 0.000265 0.000884 0.000459 0.009081
None 0.001091 0.002245 0.000945 0.021551 0.000502 0.001960 0.000748 0.001600 0.000946 0.002113 0.000845 0.020120
Nuclear Periphery 0.000092 0.000043 0.000085 0.000293 0.000009 0.000130 0.000129 0.000186 0.000072 0.000083 0.000107 0.000286
Nuclear Periphery Foci 0.000137 0.000144 0.000022 0.000456 0.000010 0.000418 0.000093 0.000045 0.000106 0.000271 0.000058 0.000427
Nucleolus 0.104863 0.120824 0.080886 0.075206 0.161510 0.142859 0.077138 0.120118 0.118757 0.131030 0.078982 0.078428
Nucleus 0.879294* 0.864440* 0.896539* 0.853950* 0.822899* 0.833276* 0.904141* 0.831853* 0.865462* 0.850006* 0.900402* 0.852365*
Peroxisomes 0.001738 0.004155 0.004165 0.002632 0.009970 0.004454 0.003079 0.006341 0.003757 0.004293 0.003613 0.002898
Vacuole 0.001044 0.000819 0.000166 0.002431 0.000209 0.000879 0.000248 0.002932 0.000840 0.000847 0.000208 0.002467
Vacuole Periphery 0.000865 0.000523 0.000223 0.000313 0.000266 0.001066 0.000273 0.001583 0.000718 0.000774 0.000248 0.000404

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.34 -10.19 -9.35 -8.28 -4.95 -3.31 -7.26 -8.32 -5.83 -6.71 -3.22 -10.85 -10.38 -8.17 -6.88
Bud Neck 8.15 -13.46 -9.32 -13.05 -2.16 -10.20 -16.14 -7.46 -5.95 -3.36 3.30 -16.37 -11.37 -12.81 -3.73
Bud Site 1.55 -0.04 -3.88 -4.76 -4.14 -2.69 -6.55 -3.80 -1.82 -2.76 -0.36 -0.70 -4.48 -4.28 -4.45
Cell Periphery 1.02 2.06 -2.03 -4.92 -7.79 -3.95 -2.43 -5.34 1.11 0.20 0.47 1.32 -2.96 -5.14 -6.43
Cytoplasm -1.97 1.11 -5.76 -5.41 -5.80 -2.59 -3.18 -3.96 1.13 -1.56 -2.70 0.29 -5.78 -5.49 -5.79
Cytoplasmic Foci 0.47 1.05 -2.10 -2.60 -2.96 -2.27 -1.72 -1.28 0.59 -1.17 -1.86 0.78 -2.49 -0.28 -3.05
Eisosomes 2.93 5.31 7.16 8.83 9.52 -1.59 4.82 4.82 5.25 1.42 2.18 5.98 8.19 10.98 9.65
Endoplasmic Reticulum 8.46 7.93 -1.39 -7.49 -6.92 -4.94 1.37 -2.85 0.51 -3.37 5.98 8.85 -2.67 -6.44 -7.82
Endosome 0.65 2.27 -2.02 -2.14 -2.36 -1.94 -1.78 -1.01 -0.96 -1.00 -1.17 1.09 -1.90 -1.72 -1.97
Golgi -1.77 -3.47 -4.64 -3.38 -4.04 -1.20 -3.19 -1.15 -0.99 -1.13 -1.39 -3.37 -2.68 -1.25 -2.48
Lipid Particles 0.67 1.74 -3.62 -4.10 -4.71 -1.51 -1.35 -2.82 1.44 -0.46 -1.47 0.17 -4.00 1.10 -3.98
Mitochondria 0.57 -4.60 -6.56 -14.04 -3.65 -3.85 -7.64 -7.82 -6.25 -6.41 -1.07 -4.85 -9.20 -11.97 -8.05
Mitotic Spindle 1.81 -1.88 -5.56 -5.72 -5.14 -2.00 -3.12 -1.86 -1.36 -1.78 -1.40 -1.96 -5.80 -5.39 -5.56
None -2.40 0.41 -6.03 -5.55 -6.06 -2.19 -3.63 -4.77 0.18 -3.69 -2.86 0.43 -6.10 -5.59 -6.13
Nuclear Periphery 1.25 -0.36 -5.74 -10.69 -8.57 -2.53 -2.44 -5.87 -2.31 -2.00 -0.35 -1.33 -7.38 -7.74 -5.93
Nuclear Periphery Foci -0.17 2.30 -2.39 -2.26 -3.06 -1.90 -1.15 -2.48 1.72 0.66 -1.54 0.72 -2.56 -1.34 -2.76
Nucleolus -3.26 4.39 3.72 6.29 0.11 2.36 11.30 0.79 -0.09 -3.12 -2.99 9.76 5.70 8.05 -1.23
Nucleus 2.80 -2.20 5.30 3.19 6.77 -1.07 -10.13 1.04 1.42 4.20 3.54 -7.37 4.05 1.77 8.51
Peroxisomes -6.57 -7.51 -3.94 2.39 3.19 6.53 8.41 0.94 -2.52 -3.32 -1.64 -0.55 0.90 2.23 1.37
Vacuole 0.41 4.36 -1.84 -1.94 -2.93 -4.09 -1.06 -1.28 -0.83 -1.24 -0.24 3.99 -2.21 -2.11 -3.03
Vacuole Periphery 2.17 4.50 3.79 3.20 -2.37 -2.21 -0.30 -1.10 -0.08 -1.08 -0.31 4.18 2.74 2.22 -1.65
Standard name
Human Ortholog
Description Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Arp7

Arp7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arp7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available