Standard name | |
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Human Ortholog | |
Description | Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 7 | 6 | 6 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 2 | 2 | 2 | 1 | 6 | 3 | 4 | 1 | 6 | 2 | 2 | 26 | 42 | 52 | 17 | 14 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 8 | 7 | 2 | 1 | 2 | 1 | 2 | 0 | 0 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 |
Mitochondria | 5 | 3 | 0 | 2 | 2 | 6 | 7 | 23 | 14 | 18 | 5 | 12 | 0 | 0 | 0 | 3 | 4 | 2 | 1 | 3 | 0 | 0 | 2 | 0 |
Nucleus | 323 | 194 | 65 | 100 | 132 | 123 | 88 | 170 | 75 | 125 | 38 | 58 | 315 | 290 | 312 | 246 | 161 | 87 | 295 | 180 | 55 | 85 | 222 | 170 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 133 | 110 | 49 | 55 | 52 | 94 | 78 | 143 | 99 | 127 | 63 | 91 | 150 | 116 | 131 | 70 | 44 | 54 | 74 | 49 | 33 | 55 | 103 | 84 |
Peroxisomes | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 10 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 13 | 12 | 3 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 7 | 4 | 0 | 6 | 5 | 0 | 2 | 12 | 16 |
Unique Cell Count | 358 | 224 | 78 | 116 | 155 | 167 | 144 | 255 | 132 | 194 | 86 | 127 | 381 | 390 | 427 | 313 | 206 | 125 | 388 | 248 | 93 | 148 | 356 | 285 |
Labelled Cell Count | 465 | 310 | 117 | 161 | 188 | 231 | 179 | 350 | 195 | 287 | 111 | 174 | 504 | 482 | 525 | 354 | 232 | 153 | 388 | 248 | 93 | 148 | 356 | 285 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.9 | 17.6 | 13.8 | 12.5 | 13.5 | 11.9 | 11.3 | 11.2 | 10.5 | 10.5 | 10.0 | 9.8 | 16.3 | 15.7 | 15.3 | 18.9 | 21.4 | 19.5 | 14.7 | 14.8 | 16.6 |
Std Deviation (1e-4) | 3.7 | 5.4 | 4.4 | 3.7 | 4.6 | 3.3 | 4.1 | 4.2 | 3.8 | 4.7 | 3.6 | 3.5 | 5.6 | 6.4 | 6.1 | 8.7 | 8.0 | 7.6 | 3.5 | 3.6 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.14 | -0.02 | -0.21 | -0.28 | -0.3 | -0.39 | -0.4 | -0.47 | -0.49 | 0.25 | 0.19 | 0.16 | 0.46 | 0.64 | 0.5 | 0.1 | 0.1 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.5 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 2.7 | 3.0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.6 | 0.4 | -1.7 | -3.4 | -2.8 | -3.9 | -3.1 | -5.2 | -5.3 | -0.1 | -1.7 | -1.9 | -0.9 | -1.0 | -2.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.1 | -4.3 | -1.0 | -1.2 | -1.1 | 1.9 | 0.4 | 1.4 | 1.3 | -3.8 | -5.6 | -5.4 | -6.9 | -6.6 | -2.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.0942 | 11.3127 | 10.3257 | 11.0619 | 8.6707 | 9.6097 | 16.8693 | 13.5509 | 14.6877 | 13.8074 | 13.7733 | 14.3419 | 18.0657 | 14.5643 | 16.2777 | 15.2149 | 15.5451 | 15.3244 |
Actin | 0.015 | 0 | 0.0009 | 0 | 0.0079 | 0 | 0.0011 | 0.0002 | 0.0004 | 0 | 0.0221 | 0 | 0 | 0 | 0.0019 | 0 | 0 | 0 |
Bud | 0.0011 | 0 | 0.0002 | 0 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0015 | 0 | 0.0002 | 0.0001 | 0.0016 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0.0002 |
Bud Periphery | 0.0011 | 0 | 0.0003 | 0.0001 | 0.0013 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Site | 0.0019 | 0 | 0.0009 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0006 | 0 | 0.0001 | 0 | 0.0011 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0027 | 0 | 0.0012 | 0 | 0.0019 | 0 | 0.0014 | 0.0001 | 0.0008 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0247 | 0 | 0.0032 | 0.0002 | 0.016 | 0.0043 | 0.0054 | 0 | 0.0019 | 0 | 0.0009 | 0 | 0.0012 | 0 | 0.0006 | 0 | 0 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0017 | 0 | 0.0009 | 0 | 0.0014 | 0 | 0.0012 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 |
Endosome | 0.0196 | 0 | 0.0146 | 0.0001 | 0.0039 | 0.0001 | 0.0026 | 0 | 0.0006 | 0 | 0.0024 | 0 | 0.001 | 0 | 0.0012 | 0 | 0 | 0 |
Golgi | 0.0081 | 0 | 0.0011 | 0 | 0.0018 | 0.0001 | 0.0004 | 0 | 0.0011 | 0 | 0.0037 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 |
Lipid Particles | 0.0109 | 0 | 0.0045 | 0.0003 | 0.0122 | 0.003 | 0.002 | 0 | 0.0004 | 0 | 0.003 | 0 | 0.0014 | 0 | 0.0003 | 0 | 0 | 0 |
Mitochondria | 0.0036 | 0.0002 | 0.0038 | 0.0007 | 0.005 | 0.0002 | 0.0005 | 0.0001 | 0.0009 | 0.0001 | 0.0019 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0001 |
None | 0.0019 | 0 | 0.0008 | 0.0001 | 0.0121 | 0 | 0.0053 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0007 | 0 | 0.0002 | 0 | 0 | 0 |
Nuclear Periphery | 0.0242 | 0.0004 | 0.0056 | 0.0007 | 0.0056 | 0.0001 | 0.0064 | 0.0062 | 0.0003 | 0.0002 | 0.0013 | 0 | 0.0003 | 0.0005 | 0.001 | 0.0002 | 0.0003 | 0.0001 |
Nucleolus | 0.1218 | 0.0563 | 0.0519 | 0.1451 | 0.4769 | 0.2962 | 0.0652 | 0.0548 | 0.027 | 0.0346 | 0.3161 | 0.1838 | 0.0665 | 0.0564 | 0.0392 | 0.0574 | 0.3567 | 0.1999 |
Nucleus | 0.7233 | 0.9426 | 0.9048 | 0.8493 | 0.4168 | 0.6945 | 0.8925 | 0.9377 | 0.9584 | 0.965 | 0.6439 | 0.8154 | 0.9266 | 0.9427 | 0.9512 | 0.942 | 0.641 | 0.7993 |
Peroxisomes | 0.0066 | 0 | 0.0007 | 0.0001 | 0.0015 | 0.0001 | 0.0011 | 0 | 0.0062 | 0 | 0.0017 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0236 | 0.0001 | 0.0017 | 0.0025 | 0.0284 | 0.0005 | 0.0138 | 0.0002 | 0.001 | 0.0001 | 0.001 | 0.0001 | 0.0006 | 0.0002 | 0.0033 | 0.0003 | 0.0012 | 0.0002 |
Vacuole | 0.0033 | 0.0001 | 0.0013 | 0.0002 | 0.0013 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 |
Vacuole Periphery | 0.0028 | 0.0001 | 0.0013 | 0.0003 | 0.0011 | 0.0002 | 0.0002 | 0.0004 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.473 | 42.6467 | 31.1353 | 27.7899 | 28.9315 | 38.4517 | 36.5129 | 30.5113 | 33.0784 | 39.1083 |
Translational Efficiency | 1.0607 | 1.0444 | 0.8411 | 1.0903 | 1.2617 | 0.8641 | 1.1814 | 0.9556 | 0.9871 | 0.9388 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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