Standard name
Human Ortholog
Description Subunit H of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.97 0.76 0.94 0.97 0.92 0.8 0.71 0.71 0.6 0.53 0.5 0.46 1.0 1.0 1.0 0.89 – 0.9 0.68 0.5 0.52 0.78 0.78 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 – 0 0 0.06 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0.05 0.06 0.11 0.05 0.34 0.5 0.44 0.65 0.67 0.68 0.7 0 0 0 0.1 – 0 0.12 0.11 0.29 0.1 0.08 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.16 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 4 1 11 3 6 9
Bud 1 2 2 0 0 12 4 8 7 13 21 15 0 0 0 0 – 0 0 3 4 1 11 12
Bud Neck 0 3 2 1 0 1 3 3 1 0 0 1 2 4 6 4 – 2 2 1 3 2 3 6
Bud Site 0 0 0 0 0 0 0 2 0 5 2 3 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 1 3 0 3 1 2 1 0 2 2 1 12 3 3 2 – 1 0 0 6 5 9 10
Cytoplasm 148 48 340 279 351 322 350 384 200 229 191 185 276 416 467 55 – 56 150 56 200 289 419 517
Endoplasmic Reticulum 1 0 2 0 0 1 1 0 0 0 2 0 1 1 1 4 – 2 9 7 10 2 1 3
Endosome 0 1 3 1 13 4 1 2 2 0 0 1 0 0 0 2 – 1 7 2 11 3 3 6
Golgi 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 – 0 0 0 1 0 1 0
Mitochondria 5 3 20 33 18 138 244 238 220 286 257 283 0 0 0 6 – 2 25 12 112 37 43 47
Nucleus 0 2 1 1 0 2 1 3 4 1 2 2 0 0 0 0 – 0 0 2 0 0 0 0
Nuclear Periphery 0 5 0 0 0 1 0 1 0 1 0 0 0 0 0 0 – 0 0 0 1 0 1 0
Nucleolus 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 7 0 0 4 0 0 0 3 0 0 0 0 – 0 0 1 0 0 0 0
SpindlePole 0 0 1 3 1 10 14 9 10 14 9 14 0 0 0 0 – 0 1 0 7 2 9 9
Vac/Vac Membrane 0 5 2 0 15 4 3 4 4 1 5 2 0 0 0 1 – 1 6 17 4 9 9 12
Unique Cell Count 152 63 360 287 380 403 491 539 336 430 380 405 277 417 468 62 62 220 114 387 369 537 645
Labelled Cell Count 155 73 376 319 408 496 623 659 448 553 492 510 291 424 477 74 65 220 114 387 369 537 645


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 8.0 3.5 3.6 3.5 2.9 2.6 3.0 2.6 2.4 2.6 2.5 4.6 4.7 4.9 5.0 4.7 5.9 4.5 4.7 5.0
Std Deviation (1e-4) 0.6 13.8 1.2 1.5 1.5 1.3 1.1 1.6 1.2 0.9 1.4 0.7 0.7 0.7 0.9 2.2 1.7 2.9 1.2 1.2 1.2
Intensity Change (Log2) – – – 0.03 -0.0 -0.28 -0.4 -0.24 -0.41 -0.52 -0.44 -0.5 0.39 0.44 0.48 0.51 0.43 0.75 0.38 0.43 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cytoplasm 1.7 -1.1 -5.9 -8.5 -8.6 -11.0 -12.8 -13.3 -14.5 3.6 4.6 4.8 -1.7 – -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 -5.2 0 – 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 3.1 0 0 0 0 0 0 0 0 0 0 0 – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 36.3398 45.9498 43.3691 41.5312 35.345 39.3659 41.901 51.2205 46.7932 44.2565 41.1139 46.1576 44.6329 55.0942 53.0475 51.7017 43.5514 43.7614
Actin 0.0019 0 0.0103 0 0.0001 0.0003 0.0113 0 0.0033 0 0.0072 0 0.0001 0 0 0 0 0.0007
Bud 0.0006 0.0001 0.0007 0.0004 0.0002 0.0006 0.0001 0 0.0021 0.0001 0.0008 0.0001 0.0018 0 0.0001 0.0002 0.0001 0.0003
Bud Neck 0.0003 0 0.0002 0 0 0.0002 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0.0002
Bud Periphery 0.0013 0.0001 0.0014 0.0005 0.0003 0.0011 0.0003 0 0.0028 0.0004 0.0017 0.0003 0.0038 0 0.0005 0.0008 0.0004 0.0008
Bud Site 0.0025 0.0001 0.0012 0.0001 0.0002 0.0009 0.0005 0 0.0011 0.0001 0.0004 0.0001 0.0007 0 0 0 0.0001 0.0002
Cell Periphery 0.0051 0.0016 0.0028 0.0021 0.0024 0.002 0.0017 0.0011 0.0025 0.0015 0.0029 0.0013 0.0042 0.0012 0.0033 0.0033 0.0023 0.0021
Cytoplasm 0.0023 0.0004 0.0036 0.0003 0.0005 0.0087 0.002 0 0.0006 0.0004 0.0008 0.0002 0.0002 0.0001 0.0001 0 0.0001 0.0019
Cytoplasmic Foci 0.0057 0.0001 0.0038 0.0002 0.0302 0.0128 0.0094 0 0.0037 0.0076 0.0106 0.0159 0.0001 0 0 0 0.0003 0.0068
Eisosomes 0 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0.0002
Endoplasmic Reticulum 0.0011 0 0.001 0 0.0001 0.0011 0.0011 0.0001 0.0003 0.0001 0.001 0 0.0001 0 0.0001 0 0.0002 0.0007
Endosome 0.0058 0.0008 0.005 0.001 0.001 0.0024 0.0123 0.0005 0.0022 0.0008 0.0258 0.0007 0.0019 0.0008 0.0013 0.002 0.0006 0.0056
Golgi 0.0006 0 0.0011 0 0.0001 0.0002 0.0081 0 0.0023 0 0.0152 0.0001 0.0001 0 0.0001 0.0001 0 0.0006
Lipid Particles 0.004 0.0001 0.0009 0.0003 0.0057 0.0027 0.0083 0.0001 0.0028 0.002 0.0097 0.0026 0.0006 0.0003 0.0014 0.0009 0.0023 0.0056
Mitochondria 0.0092 0.0002 0.0027 0.0006 0.0112 0.0025 0.0087 0.0002 0.0069 0.0017 0.0032 0.0028 0.0034 0.0006 0.0185 0.0056 0.0014 0.006
None 0.0012 0 0.0008 0 0.004 0.0042 0.0014 0 0.0012 0.0008 0.0007 0.0006 0.0001 0 0 0 0.0001 0.0013
Nuclear Periphery 0.0183 0.0048 0.0056 0.0002 0.0019 0.0101 0.0073 0.0027 0.0051 0.0051 0.0022 0.0052 0.0077 0.0045 0.002 0.0014 0.0139 0.0397
Nucleolus 0.0147 0.0015 0.003 0.0014 0.0292 0.0037 0.0067 0.0009 0.0033 0.0174 0.0023 0.0099 0.0131 0.0008 0.0015 0.0032 0.0497 0.0081
Nucleus 0.0075 0.0021 0.0029 0.0008 0.0095 0.0058 0.0021 0.0005 0.0016 0.0076 0.001 0.0018 0.0053 0.0013 0.001 0.0012 0.0065 0.0053
Peroxisomes 0.0005 0 0.0009 0.0001 0.0016 0.0005 0.0019 0 0.0006 0.0002 0.0111 0.0003 0.0001 0 0.0001 0 0.0001 0.0004
Punctate Nuclear 0.0012 0 0.0006 0 0.0011 0.0008 0.0051 0 0.0027 0.0032 0.0007 0.0009 0 0 0 0 0.0001 0.0041
Vacuole 0.2689 0.3195 0.2815 0.3185 0.234 0.1777 0.1994 0.2027 0.2177 0.2173 0.1917 0.1227 0.2317 0.2282 0.2374 0.2636 0.1692 0.1452
Vacuole Periphery 0.6474 0.6684 0.67 0.6735 0.6667 0.7615 0.7121 0.7911 0.7372 0.7339 0.7108 0.8346 0.7249 0.7622 0.7326 0.7175 0.7526 0.7643

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 200.4096 220.3387 172.1598 216.1983 206.5776 168.0667 189.6442 200.72 218.7177 213.6261
Translational Efficiency 1.3618 1.4054 1.746 1.2832 1.7248 1.8113 1.4401 1.4966 1.452 1.5025

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
107 1013 2320 981 328 1589 2170 1476 435 2602 4490 2457

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3056.94 2786.18 4367.77 3499.72 2507.14 3116.84 3777.89 3475.23 2642.38 2988.11 4082.68 3485.01
Standard Deviation 673.44 972.10 907.10 1179.56 583.85 763.71 803.85 949.25 651.65 866.07 907.93 1047.36
Intensity Change Log 2 — -0.133800 0.514809 0.195151 — 0.314042 0.591538 0.471066 — 0.085310 0.549888 0.326040

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000070 0.000066 0.000032 0.000355 0.000053 0.000039 0.000056 0.000149 0.000057 0.000049 0.000043 0.000231
Bud Neck 0.001972 0.000256 0.000397 0.003133 0.000110 0.000389 0.000641 0.000899 0.000568 0.000338 0.000515 0.001791
Bud Site 0.000663 0.001645 0.000208 0.003263 0.000611 0.000511 0.000404 0.001815 0.000624 0.000953 0.000303 0.002393
Cell Periphery 0.000277 0.002408 0.000920 0.002506 0.001030 0.002050 0.002564 0.002387 0.000845 0.002189 0.001714 0.002435
Cytoplasm 0.000061 0.002931 0.000020 0.000726 0.000154 0.000089 0.000029 0.000730 0.000131 0.001195 0.000024 0.000728
Cytoplasmic Foci 0.002830 0.001191 0.000551 0.004344 0.001174 0.000364 0.000839 0.001592 0.001581 0.000686 0.000690 0.002691
Eisosomes 0.000035 0.000020 0.000075 0.000183 0.000005 0.000030 0.000180 0.000121 0.000012 0.000026 0.000125 0.000146
Endoplasmic Reticulum 0.000044 0.000177 0.000099 0.000473 0.000095 0.000146 0.000149 0.000642 0.000083 0.000158 0.000123 0.000575
Endosome 0.000255 0.000958 0.000112 0.002663 0.000449 0.000398 0.000230 0.001060 0.000401 0.000616 0.000169 0.001700
Golgi 0.002945 0.005080 0.000329 0.004420 0.009192 0.000514 0.000583 0.003765 0.007656 0.002291 0.000451 0.004027
Lipid Particles 0.000373 0.000547 0.001134 0.002111 0.000180 0.000793 0.002199 0.002671 0.000228 0.000697 0.001649 0.002447
Mitochondria 0.002369 0.001046 0.001095 0.011392 0.003340 0.000623 0.003615 0.010235 0.003101 0.000788 0.002313 0.010697
Mitotic Spindle 0.000037 0.000049 0.000006 0.001891 0.000221 0.000028 0.000009 0.000072 0.000176 0.000036 0.000007 0.000798
None 0.000213 0.000732 0.000229 0.001075 0.000164 0.000236 0.000427 0.001233 0.000176 0.000429 0.000325 0.001170
Nuclear Periphery 0.000652 0.000701 0.000778 0.001477 0.000149 0.000212 0.000730 0.001170 0.000273 0.000402 0.000755 0.001293
Nuclear Periphery Foci 0.000468 0.000589 0.000357 0.001459 0.000148 0.000188 0.000392 0.000668 0.000227 0.000344 0.000374 0.000984
Nucleolus 0.018080 0.002221 0.005631 0.008873 0.002629 0.001456 0.006207 0.006134 0.006430 0.001754 0.005910 0.007227
Nucleus 0.001466 0.001230 0.000693 0.003595 0.000293 0.000325 0.000939 0.000580 0.000581 0.000677 0.000812 0.001784
Peroxisomes 0.000426 0.000045 0.000076 0.000431 0.000144 0.000041 0.000108 0.000142 0.000213 0.000042 0.000091 0.000258
Vacuole 0.251795* 0.187912 0.262597* 0.276777* 0.069086 0.221675* 0.343497* 0.291607* 0.114028 0.208531* 0.301695* 0.285686*
Vacuole Periphery 0.714969* 0.790197* 0.724664* 0.668855* 0.910773* 0.769892* 0.636203* 0.672326* 0.862610* 0.777797* 0.681911* 0.670940*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.43 1.39 -4.62 -5.73 -6.46 0.40 -0.10 -2.55 -4.42 -4.20 0.39 0.66 -5.14 -7.20 -7.81
Bud Neck 1.37 1.29 -0.31 -5.51 -5.26 -1.40 -6.09 -5.18 -1.93 -1.58 0.90 0.58 -2.12 -5.64 -5.56
Bud Site -1.28 1.53 -3.31 -2.24 -4.59 0.17 0.52 -2.02 -2.71 -3.19 -0.78 1.14 -3.68 -3.47 -5.62
Cell Periphery -6.60 -7.65 -4.59 -0.21 -3.29 -2.94 -4.58 -3.24 -0.72 0.36 -5.17 -4.35 -4.85 -0.71 -2.25
Cytoplasm -3.31 1.83 -2.48 1.85 -2.59 0.80 1.67 -1.04 -1.16 -1.26 -3.11 1.91 -1.99 0.55 -2.29
Cytoplasmic Foci 1.40 1.90 -1.05 -4.23 -5.20 1.18 0.49 -0.60 -4.62 -2.95 1.56 1.59 -1.76 -5.79 -6.02
Eisosomes 1.61 -3.04 -3.84 -4.47 -2.95 -5.38 -14.94 -10.57 -8.10 3.78 -3.08 -15.59 -8.14 -7.41 -1.23
Endoplasmic Reticulum -4.02 -2.83 -3.70 -2.51 -3.27 -1.87 -2.49 -1.29 -1.17 -1.17 -3.43 -2.46 -1.91 -1.61 -1.76
Endosome -2.20 1.23 -3.90 -2.57 -4.21 0.18 1.22 -1.84 -1.94 -2.96 -1.01 1.69 -4.00 -3.24 -5.19
Golgi -0.88 1.14 -0.61 0.45 -3.86 4.35 4.32 2.51 -3.86 -3.80 3.25 4.48 2.08 -2.32 -5.45
Lipid Particles -1.99 -7.81 -7.72 -7.37 -4.59 -9.14 -19.72 -14.00 -10.22 -2.37 -9.42 -22.16 -16.00 -12.55 -5.58
Mitochondria 1.38 1.38 -4.81 -6.28 -6.32 1.28 -0.13 -2.79 -7.62 -4.86 1.43 0.49 -4.02 -9.88 -8.16
Mitotic Spindle -0.32 1.44 -2.42 -2.41 -2.46 0.92 1.02 0.71 -1.11 -2.05 0.89 1.08 -1.87 -2.48 -2.57
None -3.43 -0.40 -5.11 -1.69 -5.10 -0.98 -4.09 -2.91 -2.73 -2.22 -3.25 -3.14 -4.35 -3.19 -3.78
Nuclear Periphery -0.07 -0.53 -2.45 -1.27 -2.03 -1.40 -3.36 -4.98 -4.77 -1.68 -0.59 -3.70 -5.73 -3.29 -2.59
Nuclear Periphery Foci -0.32 0.88 -1.92 -1.41 -2.17 -0.72 -3.97 -5.44 -4.73 -2.69 -0.81 -2.68 -3.56 -2.53 -2.86
Nucleolus 1.84 1.45 1.04 -5.59 -2.79 0.49 -1.46 -1.38 -5.42 0.08 1.69 0.24 -0.27 -7.80 -1.85
Nucleus 0.65 1.22 -1.53 -2.59 -3.17 -0.12 -2.37 -1.06 -2.41 2.87 0.04 -0.35 -2.45 -2.91 -2.71
Peroxisomes 2.10 1.93 -0.02 -3.71 -3.41 0.93 0.33 0.02 -4.38 -1.67 1.84 1.34 -0.45 -4.84 -3.81
Vacuole 3.24 -0.54 -1.23 -9.44 -1.67 -19.02 -34.66 -27.19 -8.94 6.73 -11.12 -22.70 -19.83 -12.80 2.81
Vacuole Periphery -3.35 -0.57 1.96 11.18 5.99 14.52 28.58 23.68 11.60 -4.33 8.37 18.38 18.52 16.06 1.99
Standard name
Human Ortholog
Description Subunit H of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
Localization
Cell Percentages vacuolar membrane (45%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Vma13

Vma13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available