Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 0.99 0.98 0.93 0.88 0.8 0.73 0.71 0.69 0.97 1.0 0.98 0.99 0.99 0.98 0.93 0.93 0.91 0.82 0.83 0.8
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.1 0.17 0.22 0.22 0.25 0 0 0 0.06 0 0.05 0 0 0 0.07 0.1 0.14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 1 0 5 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 1 1 7 1 11 3 10 4 14 0 1 2 0 2 1 0 0 0 0 0 0
Cytoplasm 43 118 92 142 197 137 260 116 177 150 147 72 126 108 257 149 189 60 121 78 76 107 130
Endoplasmic Reticulum 0 1 0 2 2 11 28 24 53 47 53 2 0 2 15 5 9 0 1 2 6 12 22
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 1 4 7 11 7 0 0 0 1 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1 0 9 0 1 0 0 1 1
Nucleus 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 0 0 0 1 1 0 0 0 3 0 0 0 0 0 0 1 1
Unique Cell Count 43 119 92 144 200 148 294 145 241 211 213 74 126 110 259 151 192 65 131 86 93 129 163
Labelled Cell Count 43 120 93 145 207 151 302 151 248 213 228 74 127 112 277 157 208 65 131 86 93 129 163


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 198.0 204.8 159.9 166.7 137.1 82.4 93.2 69.9 71.6 64.2 65.2 208.3 209.3 199.8 214.6 233.3 234.1 179.3 193.0 215.7
Std Deviation (1e-4) 58.0 54.4 35.0 33.5 26.7 25.1 20.8 21.1 17.0 15.5 18.2 30.1 45.3 40.3 78.8 76.4 82.4 63.0 66.9 62.9
Intensity Change (Log2) 0.06 -0.22 -0.96 -0.78 -1.19 -1.16 -1.32 -1.29 0.38 0.39 0.32 0.42 0.55 0.55 0.17 0.27 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -1.2 -2.7 -3.4 -4.6 -5.5 -5.8 -6.0 -1.6 0 -1.3 -0.8 -1.1 -1.2
Endoplasmic Reticulum 0 0 0 3.1 4.1 4.9 4.9 5.3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 169.4287 239.4328 221.3229 254.4759 96.3659 202.7262 225.9288 288.5336 271.7855 280.2598 272.2735 255.193 216.3989 263.6384 230.5723 197.2896 191.7855 278.7722
Actin 0.0231 0.0015 0.0116 0.0012 0.0309 0.0231 0.0117 0.0026 0.0052 0.0019 0.0049 0.0205 0.005 0.0033 0.0135 0.0021 0.0047 0.0123
Bud 0.0054 0.0044 0.0054 0.0071 0.015 0.0182 0.0052 0.0063 0.0068 0.0281 0.0182 0.0214 0.007 0.0077 0.0069 0.0085 0.0113 0.0105
Bud Neck 0.0014 0.0015 0.0022 0.0026 0.0013 0.0042 0.002 0.002 0.0021 0.0019 0.0053 0.0041 0.0022 0.002 0.0092 0.0012 0.0026 0.0014
Bud Periphery 0.0102 0.0052 0.006 0.0088 0.031 0.0274 0.009 0.0071 0.0063 0.0156 0.0123 0.0226 0.0132 0.0086 0.008 0.0103 0.0085 0.0182
Bud Site 0.0032 0.0043 0.0044 0.0022 0.0039 0.0044 0.0034 0.0059 0.0059 0.0041 0.003 0.0028 0.0038 0.0057 0.0089 0.002 0.002 0.0011
Cell Periphery 0.1563 0.1288 0.1641 0.1237 0.051 0.082 0.1542 0.1266 0.12 0.0935 0.0272 0.0405 0.1517 0.0668 0.1355 0.0651 0.0181 0.0567
Cytoplasm 0.2005 0.4553 0.2486 0.0605 0.0476 0.2493 0.2384 0.4901 0.4803 0.3634 0.5614 0.2659 0.237 0.6513 0.2989 0.2148 0.2219 0.4582
Cytoplasmic Foci 0.007 0.0015 0.0023 0.0005 0.0451 0.0037 0.0017 0.0016 0.0014 0.001 0.0017 0.0035 0.0012 0.0018 0.0144 0.0018 0.0225 0.0008
Eisosomes 0.0041 0.0012 0.0013 0.0002 0.0009 0.0009 0.0148 0.0008 0.0135 0.0001 0 0.001 0.0078 0.0001 0.0012 0.0001 0.0032 0.0002
Endoplasmic Reticulum 0.0414 0.0295 0.0081 0.0014 0.0378 0.0124 0.023 0.0066 0.0031 0.0017 0.0181 0.0607 0.0358 0.006 0.0402 0.0171 0.0042 0.0099
Endosome 0.0097 0.0047 0.0063 0.0028 0.1199 0.0223 0.0037 0.0022 0.0022 0.0016 0.0051 0.0088 0.0062 0.0034 0.0141 0.0082 0.0139 0.0069
Golgi 0.0027 0.0033 0.0019 0.0003 0.0534 0.0052 0.0012 0.0004 0.0005 0.0002 0.0006 0.0031 0.002 0.0009 0.0065 0.0006 0.002 0.0034
Lipid Particles 0.0069 0.004 0.0056 0.0014 0.0147 0.0049 0.0044 0.0032 0.0034 0.0021 0.0009 0.0026 0.0032 0.0016 0.0087 0.002 0.006 0.0008
Mitochondria 0.0526 0.0095 0.0257 0.0073 0.1585 0.0501 0.0387 0.0328 0.0178 0.0085 0.0117 0.1205 0.0457 0.0096 0.0188 0.0108 0.0878 0.029
None 0.009 0.0013 0.003 0.0008 0.0026 0.0019 0.0035 0.0023 0.0018 0.0013 0.0008 0.0031 0.0029 0.001 0.0052 0.0033 0.0082 0.0015
Nuclear Periphery 0.0621 0.0092 0.0163 0.077 0.0951 0.0639 0.0506 0.0156 0.0131 0.0212 0.0304 0.0362 0.0622 0.009 0.0265 0.0302 0.023 0.0352
Nucleolus 0.0281 0.0088 0.0176 0.0128 0.0021 0.0054 0.0156 0.0218 0.0106 0.0183 0.0038 0.0127 0.0109 0.0037 0.0091 0.0301 0.1123 0.0019
Nucleus 0.029 0.0086 0.021 0.0539 0.027 0.0222 0.0366 0.0204 0.0164 0.0718 0.0352 0.0433 0.0387 0.036 0.0226 0.062 0.0314 0.0147
Peroxisomes 0.0008 0.0004 0.0006 0.0006 0.0125 0.0016 0.001 0.0011 0.0008 0.0007 0.001 0.0018 0.0006 0.0004 0.0034 0.0005 0.0044 0.0003
Punctate Nuclear 0.0008 0.0001 0.0002 0.0001 0.0025 0.0006 0.0002 0.0001 0.0001 0.0001 0.0001 0.0004 0.0002 0.0001 0.0006 0.0002 0.0004 0.0001
Vacuole 0.2565 0.2629 0.331 0.3108 0.0896 0.2533 0.2935 0.2094 0.2436 0.2543 0.1894 0.1773 0.2598 0.141 0.2578 0.3295 0.2176 0.1906
Vacuole Periphery 0.0892 0.0541 0.1168 0.324 0.1576 0.143 0.0875 0.0413 0.045 0.1085 0.0687 0.1473 0.103 0.0398 0.0898 0.1997 0.1938 0.1462

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2153.4184 2068.2081 3255.662 2334.201 3033.0002 2367.6465 2241.2979 2213.5968 2759.9273 2039.52
Translational Efficiency 1.7494 1.9938 1.3413 2.1866 1.6196 1.6582 1.6278 1.695 1.4131 1.7817

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1922 980 2458 1545 1738 2525 3191 948 3660 3505 5649 2493

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 9483.07 11220.55 15816.61 12818.50 9468.56 12106.28 14623.26 12122.71 9476.18 11858.63 15142.51 12553.92
Standard Deviation 2340.56 2632.29 3169.48 3360.89 2118.87 2788.51 3059.21 2831.56 2238.04 2774.35 3163.48 3187.98
Intensity Change Log 2 0.242717 0.738014 0.434801 0.354539 0.627048 0.356495 0.299668 0.683640 0.396210

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000054 0.000140 0.000073 0.000379 0.000065 0.000088 0.000112 0.000211 0.000059 0.000103 0.000095 0.000315
Bud Neck 0.000150 0.000432 0.000365 0.001000 0.000274 0.000188 0.000228 0.001314 0.000209 0.000256 0.000288 0.001119
Bud Site 0.001343 0.004606 0.001736 0.006005 0.001499 0.002825 0.003655 0.006237 0.001417 0.003323 0.002820 0.006093
Cell Periphery 0.060330 0.089001 0.080344 0.109450* 0.064740 0.060615 0.097777 0.087674 0.062424 0.068552 0.090192 0.101169*
Cytoplasm 0.322929* 0.219910* 0.331180* 0.231814* 0.264231* 0.388325* 0.420587* 0.243940* 0.295055* 0.341236* 0.381684* 0.236425*
Cytoplasmic Foci 0.019436 0.014831 0.008799 0.005145 0.013722 0.024272 0.010691 0.004320 0.016723 0.021632 0.009868 0.004831
Eisosomes 0.000003 0.000005 0.000004 0.000011 0.000004 0.000003 0.000004 0.000006 0.000003 0.000004 0.000004 0.000009
Endoplasmic Reticulum 0.010761 0.007165 0.011686 0.002938 0.009833 0.009552 0.008528 0.001252 0.010320 0.008884 0.009902 0.002297
Endosome 0.001382 0.001750 0.001029 0.001039 0.001302 0.001682 0.001089 0.000884 0.001344 0.001701 0.001063 0.000980
Golgi 0.080469 0.116132* 0.049009 0.033487 0.116045* 0.087792 0.026504 0.035302 0.097363 0.095716 0.036296 0.034177
Lipid Particles 0.042606 0.052580 0.036119 0.049356 0.047556 0.040749 0.033147 0.044313 0.044957 0.044057 0.034440 0.047438
Mitochondria 0.000522 0.000540 0.000510 0.001517 0.000389 0.000872 0.000900 0.000406 0.000459 0.000779 0.000730 0.001094
Mitotic Spindle 0.000670 0.000860 0.000697 0.000670 0.000803 0.001035 0.000481 0.000628 0.000733 0.000986 0.000575 0.000654
None 0.001900 0.002051 0.000925 0.003226 0.001411 0.002157 0.000716 0.000778 0.001668 0.002127 0.000807 0.002295
Nuclear Periphery 0.001345 0.001796 0.001806 0.001357 0.002096 0.001171 0.000914 0.000901 0.001702 0.001346 0.001302 0.001184
Nuclear Periphery Foci 0.011197 0.011932 0.009870 0.008519 0.013617 0.009030 0.004925 0.008516 0.012346 0.009841 0.007076 0.008517
Nucleolus 0.000217 0.000181 0.000149 0.000217 0.000125 0.000133 0.000205 0.000201 0.000173 0.000146 0.000181 0.000211
Nucleus 0.002244 0.002385 0.002209 0.007308 0.001584 0.001498 0.001519 0.004920 0.001931 0.001746 0.001819 0.006400
Peroxisomes 0.000280 0.000171 0.000496 0.000210 0.000172 0.000334 0.000969 0.000124 0.000229 0.000289 0.000763 0.000177
Vacuole 0.200508* 0.244762* 0.270913* 0.428171* 0.192157 0.159944 0.255056* 0.477935* 0.196542 0.183659 0.261956* 0.447095*
Vacuole Periphery 0.241654* 0.228770* 0.192082* 0.108181* 0.268377* 0.207736* 0.131991* 0.080140 0.254344* 0.213617* 0.158138* 0.097518

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.73 -2.73 -5.81 -4.20 -5.49 -0.64 -1.75 -2.62 -2.49 -1.97 -2.65 -2.74 -6.25 -5.37 -5.58
Bud Neck -5.56 -4.90 -11.79 -7.21 -7.74 0.97 0.60 -7.47 -10.99 -10.29 -0.64 -1.26 -12.14 -14.30 -13.09
Bud Site -5.59 -2.50 -6.40 -1.65 -5.85 -5.04 -6.91 -7.26 -5.24 -4.11 -7.86 -7.52 -9.13 -5.21 -6.41
Cell Periphery -6.55 -6.45 -11.00 -3.02 -6.03 1.51 -10.71 -3.92 -5.05 3.66 -2.67 -12.64 -11.23 -8.87 -1.88
Cytoplasm 8.70 -0.71 8.19 -1.33 9.32 -12.43 -16.43 0.89 11.27 14.43 -6.05 -12.53 6.53 11.96 18.43
Cytoplasmic Foci 2.33 7.40 8.18 5.06 2.55 -7.07 3.11 8.53 14.22 7.03 -3.82 7.68 10.89 13.95 5.83
Eisosomes -4.31 -3.26 -8.37 -5.58 -7.07 0.33 -1.18 -5.19 -5.53 -4.41 -2.93 -3.29 -9.39 -8.18 -8.14
Endoplasmic Reticulum 3.38 -0.75 10.27 5.55 11.03 0.34 1.67 13.40 15.69 13.88 2.19 0.77 15.80 14.68 16.39
Endosome -2.85 5.22 2.99 4.65 0.11 -4.72 3.34 4.98 9.15 2.80 -5.35 6.00 4.65 8.29 1.30
Golgi -7.21 11.38 17.15 18.72 8.47 7.67 28.72 23.28 18.76 -3.09 0.67 29.33 28.85 27.62 2.61
Lipid Particles -3.10 2.90 -1.93 1.46 -5.14 2.86 6.52 1.52 -0.89 -3.92 0.52 6.68 -0.52 -1.03 -6.84
Mitochondria -0.10 0.29 -3.02 -2.78 -3.08 -4.67 -5.28 -0.24 2.28 2.45 -4.10 -4.81 -2.99 -1.64 -1.91
Mitotic Spindle -3.00 -0.89 -0.61 1.47 -0.23 -1.35 8.92 1.18 1.83 -1.85 -2.09 6.39 0.46 2.09 -1.93
None -0.17 2.11 -1.63 -1.26 -3.24 -1.46 5.40 3.16 2.54 -0.55 -0.95 3.41 -1.33 -0.39 -3.35
Nuclear Periphery -1.29 -6.25 -0.31 1.22 5.79 9.73 13.40 12.58 4.03 0.04 3.21 6.59 8.36 1.40 2.57
Nuclear Periphery Foci -1.03 2.10 3.55 4.09 2.16 8.01 17.88 6.86 0.53 -6.41 5.64 14.13 7.03 2.44 -3.13
Nucleolus 0.53 1.31 -0.18 -0.87 -3.36 0.16 -1.45 -1.25 -2.11 -0.14 0.92 0.11 -0.98 -2.86 -2.19
Nucleus -0.22 -0.44 -6.04 -5.65 -6.21 0.44 -0.30 -5.86 -5.98 -5.79 0.57 -0.27 -8.08 -8.48 -8.30
Peroxisomes 2.67 -3.36 1.65 -0.64 4.58 -2.69 -7.40 1.14 5.19 8.65 -1.47 -8.07 1.70 3.32 9.29
Vacuole -5.53 -11.34 -28.25 -19.33 -19.34 5.69 -10.86 -28.18 -32.40 -22.27 2.70 -15.41 -39.94 -41.78 -29.76
Vacuole Periphery 1.94 8.97 25.25* 18.73 19.14* 10.26 25.39 34.45* 29.29* 14.89* 9.27 24.96 40.62 32.27* 21.36*
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl43a

Rpl43a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl43a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available