Standard name
Human Ortholog
Description Protein that may have a role in transcription elongation; forms a complex with Csn12p that is recruited to transcribed genes; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.08 0 0.05 0.08 0 0.07 0.05 0 0 0.13 0.17 0.15 0.05 0.08 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0.13 0.05 0.06 0.26 0.27 0.24 0.38 0.44 0.43 0.42 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.93 0.96 0.88 0.87 0.78 0.75 0.8 0.73 0.77 0.63 0.75 0.86 0.8 0.78 0.89 0.87 0.9 0.93 0.95 0.92 0.82 0.65 0.56
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.05 0.06 0.11 0.12 0.1 0.14 0.15 0.22 0.15 0 0 0.05 0 0 0 0 0 0 0 0.1 0.09
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0.09 0.19 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 1 0 0 0 0 2 1 5 1 1 1 0 0 1 0 0 1 0 0 1 0 3 11
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 4 1 6 6 10 7 4 11 1 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 3 0 10 1 9 18 14 13 11 5 6 55 97 88 16 22 9 0 0 0 2 1 3
Endoplasmic Reticulum 0 1 0 0 0 0 1 0 0 0 0 0 0 1 2 6 8 4 0 0 0 0 1 2
Endosome 0 1 0 0 5 0 1 2 0 0 0 0 3 5 4 3 1 0 1 0 0 0 4 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 1 2 0 2
Mitochondria 11 3 15 6 12 44 62 77 69 102 69 91 0 2 2 8 5 1 1 1 0 1 6 8
Nucleus 134 157 113 107 173 134 173 254 132 180 100 165 371 465 443 290 229 184 131 163 108 165 310 277
Nuclear Periphery 1 0 0 3 4 0 6 1 0 2 1 3 2 6 13 3 3 0 0 0 0 0 2 9
Nucleolus 5 3 1 6 11 19 29 33 26 35 35 33 2 20 27 5 2 2 0 0 0 7 50 43
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 5 7 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 0 0 0 0 1 4 6 2 0 1 0 0 0 1 2 6
Vac/Vac Membrane 0 5 1 3 9 5 6 5 1 2 8 7 4 6 21 15 10 6 2 3 4 17 90 112
Unique Cell Count 141 168 118 122 199 171 232 318 181 233 159 219 433 583 571 326 264 204 142 173 118 201 479 493
Labelled Cell Count 154 174 130 135 219 212 304 395 256 340 223 317 440 612 615 350 280 208 142 173 118 201 479 493


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 8.1 6.5 6.8 6.5 5.4 5.3 5.2 5.2 4.7 4.7 4.5 8.1 7.8 7.6 7.8 8.6 8.9 7.0 7.3 8.4
Std Deviation (1e-4) 0.9 1.3 1.4 1.9 2.0 1.4 2.1 1.5 1.5 1.5 1.0 1.2 2.2 2.3 2.3 1.6 1.9 1.6 1.5 1.6 1.9
Intensity Change (Log2) – – – 0.05 0.01 -0.26 -0.31 -0.32 -0.34 -0.48 -0.46 -0.52 0.31 0.26 0.22 0.25 0.4 0.45 0.1 0.17 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 3.1 0 3.0 0 0 0 4.1 4.8 4.6 0 3.2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.1 -2.1 2.7 3.0 2.6 4.8 0 0 0 0 0 0 -4.3 -4.4 -4.9
Nucleus -2.3 -2.6 -4.1 -4.8 -4.0 -5.0 -4.4 -6.4 -4.7 -3.0 -4.2 -4.6 -2.2 -2.7 -1.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.4 3.7 3.3 4.0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9825 3.4516 3.258 3.082 2.4366 3.2446 4.3959 4.4604 4.2903 4.2635 4.4863 4.5767 5.4009 6.0314 5.715 6.4582 5.4856 5.7204
Actin 0.0058 0 0.0017 0 0.0002 0.0003 0.016 0 0.0016 0.0165 0.0389 0.0004 0.0001 0 0 0 0 0
Bud 0.0001 0 0.0001 0 0 0 0.0011 0 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0 0 0 0
Bud Neck 0.0006 0 0.0002 0 0.0002 0.0006 0.0022 0 0.0005 0.0005 0.0059 0.001 0.0005 0 0 0.0001 0.0002 0.0006
Bud Periphery 0.0002 0 0.0003 0 0.0001 0.0001 0.0033 0 0.0005 0.0002 0.0008 0.0002 0.0003 0.0001 0 0 0.0001 0
Bud Site 0.0007 0 0.0001 0 0 0.0001 0.0049 0 0.0005 0.0006 0.0041 0.0001 0.0003 0.0005 0 0 0.0001 0
Cell Periphery 0.0001 0 0 0 0 0 0.0007 0 0.0002 0.0001 0.0005 0 0.0001 0 0 0 0 0
Cytoplasm 0.0027 0.0001 0.0002 0 0.0003 0.0001 0.0075 0 0.0006 0.0014 0.0035 0.0001 0.0004 0.0004 0 0.0002 0.0001 0
Cytoplasmic Foci 0.0015 0 0.0001 0 0 0 0.0146 0 0.0048 0.0101 0.0093 0.0008 0.0046 0.0003 0 0 0 0
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0 0.0001 0.0003 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0017 0 0.0003 0 0.0002 0.0001 0.0041 0 0.0009 0.0034 0.0039 0.0001 0.0005 0 0 0 0 0
Endosome 0.004 0 0.0007 0 0.0005 0.0003 0.0182 0 0.0106 0.0209 0.032 0.0009 0.0062 0 0 0 0 0
Golgi 0.0018 0 0.0002 0 0.0001 0.0001 0.0048 0 0.0027 0.0095 0.0129 0.0005 0.0003 0 0 0 0 0
Lipid Particles 0.0046 0 0.0002 0 0.0001 0.0003 0.0103 0 0.0095 0.0278 0.0174 0.0045 0.0022 0 0 0 0 0
Mitochondria 0.011 0.0001 0.003 0 0.0233 0.0005 0.0149 0.0001 0.0107 0.0341 0.0187 0.0007 0.0006 0.0001 0.0001 0.0002 0.0003 0.0001
None 0.0016 0 0.0001 0 0.0001 0.0002 0.0058 0 0.0003 0.001 0.0017 0.0001 0.0002 0.0001 0 0.0006 0 0
Nuclear Periphery 0.0116 0.0055 0.0052 0.0009 0.002 0.0024 0.0168 0.0014 0.013 0.0323 0.0052 0.0013 0.0033 0.0007 0.0006 0.0014 0.0019 0.0002
Nucleolus 0.0167 0.0078 0.0068 0.0067 0.0224 0.0361 0.0151 0.0087 0.0114 0.0084 0.024 0.0397 0.0074 0.0122 0.006 0.0101 0.0369 0.0218
Nucleus 0.9192 0.9837 0.9705 0.9918 0.9471 0.9501 0.8242 0.9893 0.9216 0.8072 0.8033 0.9457 0.9555 0.9782 0.9888 0.9863 0.9587 0.9711
Peroxisomes 0.003 0 0.0001 0 0 0.0001 0.0176 0 0.004 0.0146 0.0119 0.0019 0.0003 0 0 0 0 0
Punctate Nuclear 0.0107 0.0026 0.0096 0.0004 0.002 0.0083 0.0105 0.0003 0.0026 0.0077 0.0028 0.0013 0.0147 0.007 0.0043 0.0009 0.0014 0.0059
Vacuole 0.0013 0 0.0003 0 0.0003 0.0002 0.0049 0 0.0023 0.0018 0.0013 0.0003 0.0021 0.0001 0 0 0.0001 0.0002
Vacuole Periphery 0.0008 0.0001 0.0004 0 0.0011 0.0002 0.0023 0 0.0015 0.0016 0.0012 0.0001 0.0005 0 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.9606 16.0557 12.432 17.1182 17.2119 10.9265 14.1164 17.3879 16.412 20.2599
Translational Efficiency 1.3749 1.2569 1.5461 0.9998 1.3299 2.7199 1.6781 1.2602 1.3347 1.2688

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2063 1355 2479 1854 661 1118 1177 86 2724 2473 3656 1940

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 801.10 915.17 1023.61 1056.04 853.28 868.27 1036.43 1175.36 813.76 893.97 1027.74 1061.33
Standard Deviation 101.04 147.76 142.99 154.43 111.21 131.95 142.16 178.07 105.98 142.75 142.85 157.48
Intensity Change Log 2 — 0.192057 0.353612 0.398610 — 0.025125 0.280532 0.462012 — 0.108372 0.316382 0.431659

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000175 0.000435 0.000355 0.000624 0.000237 0.000546 0.000540 0.002033 0.000190 0.000485 0.000414 0.000686
Bud Neck 0.002542 0.007732 0.016872 0.031388 0.002501 0.007278 0.020717 0.017790 0.002532 0.007527 0.018110 0.030786
Bud Site 0.000371 0.001294 0.000867 0.004923 0.000259 0.001962 0.001292 0.027759 0.000344 0.001596 0.001003 0.005935
Cell Periphery 0.000185 0.000166 0.000110 0.000178 0.000115 0.000168 0.000148 0.000320 0.000168 0.000167 0.000123 0.000184
Cytoplasm 0.001693 0.001190 0.000705 0.006022 0.000435 0.002416 0.002108 0.009718 0.001388 0.001744 0.001157 0.006186
Cytoplasmic Foci 0.000139 0.000374 0.000015 0.000545 0.000025 0.001120 0.000080 0.020756 0.000111 0.000711 0.000036 0.001441
Eisosomes 0.000019 0.000030 0.000045 0.000026 0.000019 0.000034 0.000028 0.000043 0.000019 0.000032 0.000039 0.000027
Endoplasmic Reticulum 0.004586 0.004865 0.002989 0.004329 0.003136 0.007561 0.004916 0.006196 0.004234 0.006084 0.003609 0.004412
Endosome 0.000210 0.000535 0.000074 0.001556 0.000087 0.001019 0.000358 0.030717 0.000180 0.000754 0.000165 0.002849
Golgi 0.000139 0.000482 0.000025 0.002939 0.000031 0.000562 0.000073 0.024074 0.000113 0.000518 0.000041 0.003876
Lipid Particles 0.000145 0.000291 0.000013 0.000087 0.000050 0.000934 0.000051 0.001291 0.000122 0.000582 0.000025 0.000140
Mitochondria 0.000356 0.001876 0.000664 0.002735 0.000334 0.006595 0.001219 0.005898 0.000351 0.004009 0.000842 0.002875
Mitotic Spindle 0.000323 0.000601 0.000380 0.010783 0.000040 0.005251 0.002220 0.067505 0.000254 0.002703 0.000972 0.013297
None 0.004072 0.004016 0.000725 0.004763 0.002904 0.003719 0.002806 0.009200 0.003788 0.003882 0.001395 0.004960
Nuclear Periphery 0.000165 0.000265 0.000180 0.001879 0.000098 0.000455 0.000533 0.003563 0.000149 0.000351 0.000294 0.001954
Nuclear Periphery Foci 0.000251 0.000292 0.000016 0.000716 0.000059 0.000990 0.000176 0.012124 0.000204 0.000608 0.000068 0.001221
Nucleolus 0.008881 0.010090 0.004613 0.003809 0.019300 0.010652 0.005775 0.003299 0.011409 0.010344 0.004987 0.003786
Nucleus 0.975084* 0.963723* 0.970762* 0.914241* 0.969884* 0.944315* 0.954865* 0.702263* 0.973822* 0.954949* 0.965644* 0.904844*
Peroxisomes 0.000095 0.000886 0.000078 0.000135 0.000240 0.001157 0.000093 0.010507 0.000130 0.001009 0.000083 0.000595
Vacuole 0.000425 0.000554 0.000439 0.005319 0.000170 0.002108 0.001517 0.032392 0.000363 0.001257 0.000786 0.006519
Vacuole Periphery 0.000145 0.000304 0.000076 0.003003 0.000076 0.001157 0.000487 0.012553 0.000128 0.000690 0.000208 0.003426

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.56 -8.59 -11.43 -6.17 -8.06 -5.05 -12.67 -5.54 -4.79 -4.69 -8.15 -13.72 -12.46 -6.48 -7.96
Bud Neck -6.77 -18.29 -19.82 -15.29 -9.48 -4.23 -12.19 -6.28 -5.18 -1.56 -8.48 -22.14 -20.33 -16.46 -8.91
Bud Site -2.05 -4.42 -6.95 -3.92 -5.81 -3.76 -7.79 -2.90 -2.60 -2.70 -3.95 -7.67 -7.49 -4.51 -6.08
Cell Periphery 0.39 1.80 1.48 1.15 -0.60 -0.90 -0.30 -1.69 -1.17 -1.84 0.02 1.48 1.10 1.37 -0.56
Cytoplasm 0.88 1.98 -5.12 -5.59 -6.34 -2.93 -4.61 -3.29 -2.53 -2.55 -0.72 0.32 -5.95 -5.42 -6.20
Cytoplasmic Foci -1.42 1.90 -1.53 0.42 -3.41 -3.03 -1.19 -1.97 -1.63 -1.96 -3.17 1.55 -2.64 0.58 -3.19
Eisosomes -4.42 -8.10 -4.43 0.91 5.66 -4.98 -5.89 -5.08 -3.26 -4.06 -6.32 -9.05 -5.94 1.34 4.52
Endoplasmic Reticulum -0.83 5.26 -0.51 0.38 -6.74 -7.86 -6.40 -4.84 -1.73 -3.46 -5.58 2.10 -2.64 3.24 -5.18
Endosome -1.39 2.32 -3.94 -1.44 -4.87 -2.82 -3.13 -2.12 -1.93 -2.07 -2.89 0.25 -4.18 -1.83 -4.25
Golgi -1.03 1.27 -1.97 -1.32 -2.16 -2.85 -1.91 -1.55 -1.44 -1.54 -1.98 1.03 -2.41 -1.75 -2.53
Lipid Particles -0.84 1.26 0.03 1.09 -5.91 -2.13 -1.24 -2.69 -0.36 -2.65 -2.13 1.15 -0.79 1.94 -5.56
Mitochondria -1.92 -3.41 -11.79 -1.28 -10.72 -3.58 -10.83 -3.18 1.27 -2.23 -4.05 -6.92 -12.63 1.14 -10.06
Mitotic Spindle -0.79 -0.25 -6.24 -6.10 -6.19 -3.57 -2.67 -2.75 -2.33 -2.53 -3.49 -2.20 -6.85 -5.01 -6.20
None 0.06 4.84 -0.06 -0.10 -4.73 -0.63 0.18 -1.42 -1.15 -1.53 -0.09 4.11 -0.54 -0.40 -4.05
Nuclear Periphery -1.37 -1.54 -8.28 -7.52 -8.15 -3.98 -8.73 -3.75 -3.25 -2.95 -3.53 -7.85 -8.80 -7.68 -7.87
Nuclear Periphery Foci -0.18 1.53 -1.32 -1.16 -3.05 -2.09 -1.72 -1.77 -1.37 -1.73 -1.68 1.15 -2.29 -0.35 -3.25
Nucleolus -0.90 3.93 3.33 3.73 -0.38 4.28 7.87 3.61 0.91 -0.91 0.95 7.34 6.10 4.85 -0.30
Nucleus 3.48 3.12 15.09 10.86 13.90 4.99 5.73 5.64 4.76 5.01 6.46 6.19 15.98 10.06 13.48
Peroxisomes -1.53 0.55 -1.45 1.47 -2.27 -2.49 5.40 -1.59 -1.14 -1.65 -2.70 2.64 -1.56 2.13 -1.95
Vacuole -1.00 -0.79 -7.92 -7.75 -7.72 -4.31 -8.78 -3.78 -3.59 -3.61 -4.08 -5.05 -8.68 -7.46 -7.95
Vacuole Periphery -1.51 1.30 -2.87 -2.51 -3.02 -2.97 -3.65 -2.19 -1.61 -2.01 -3.18 -1.23 -3.25 -1.96 -3.13
Standard name
Human Ortholog
Description Protein that may have a role in transcription elongation; forms a complex with Csn12p that is recruited to transcribed genes; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Thp3

Thp3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Thp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available