Standard name | |
---|---|
Human Ortholog | |
Description | Adapter protein for pexophagy and the Cvt targeting pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the PAS; recruits Dnm1p to facilitate fission of mitochondria that are destined for removal by mitophagy |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 1 | 7 | 2 | 4 | 2 | 2 | 6 |
Bud | 6 | 1 | 4 | 7 | 9 | 9 | 16 | 30 | 7 | 18 | 9 | 20 | 3 | 3 | 7 | 2 | – | 1 | 3 | 0 | 0 | 1 | 0 | 2 |
Bud Neck | 6 | 0 | 7 | 4 | 5 | 11 | 10 | 14 | 2 | 3 | 0 | 2 | 0 | 0 | 0 | 1 | – | 0 | 4 | 1 | 1 | 1 | 1 | 2 |
Bud Site | 1 | 0 | 0 | 2 | 0 | 0 | 3 | 5 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | – | 1 | 1 | 0 | 2 | 0 | 0 | 0 |
Cytoplasm | 289 | 55 | 149 | 205 | 284 | 266 | 347 | 378 | 175 | 218 | 83 | 148 | 95 | 127 | 190 | 43 | – | 41 | 224 | 62 | 98 | 43 | 127 | 129 |
Endoplasmic Reticulum | 2 | 1 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 3 | 1 | 0 | 3 | 3 | 5 | – | 2 | 3 | 1 | 2 | 0 | 1 | 3 |
Endosome | 23 | 1 | 0 | 0 | 8 | 1 | 1 | 5 | 0 | 2 | 0 | 0 | 13 | 16 | 8 | 7 | – | 13 | 39 | 4 | 10 | 11 | 18 | 21 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 3 |
Mitochondria | 9 | 23 | 12 | 35 | 16 | 72 | 124 | 150 | 90 | 138 | 86 | 154 | 3 | 0 | 8 | 6 | – | 1 | 45 | 17 | 40 | 3 | 15 | 16 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 4 | 0 | 2 | 1 | 1 | 4 | 0 | 1 | 6 | 2 | 1 | 2 | – | 1 | 3 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 61 | 4 | 14 | 12 | 25 | 26 | 33 | 59 | 15 | 27 | 7 | 22 | 11 | 14 | 4 | 4 | – | 6 | 62 | 18 | 20 | 14 | 18 | 24 |
Vac/Vac Membrane | 3 | 1 | 0 | 0 | 2 | 1 | 2 | 3 | 0 | 2 | 0 | 3 | 0 | 0 | 2 | 5 | – | 5 | 7 | 3 | 4 | 1 | 8 | 10 |
Unique Cell Count | 379 | 78 | 176 | 233 | 328 | 342 | 458 | 546 | 247 | 347 | 152 | 269 | 118 | 153 | 210 | 64 | 63 | 413 | 117 | 194 | 82 | 201 | 227 | |
Labelled Cell Count | 404 | 88 | 187 | 266 | 356 | 391 | 544 | 651 | 292 | 416 | 190 | 357 | 132 | 166 | 227 | 77 | 73 | 413 | 117 | 194 | 82 | 201 | 227 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 3.4 | 3.1 | 2.9 | 3.7 | 2.9 | 2.9 | 3.2 | 2.6 | 2.7 | 2.6 | 2.7 | 4.3 | 4.4 | 3.9 | 13.1 | – | 8.8 | 4.1 | 4.1 | 5.2 |
Std Deviation (1e-4) | 0.8 | 0.8 | 0.5 | 1.0 | 1.7 | 0.8 | 1.2 | 1.3 | 1.2 | 1.0 | 0.5 | 1.3 | 1.1 | 0.8 | 1.1 | 28.1 | – | 15.9 | 1.2 | 1.2 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.24 | -0.08 | -0.11 | 0.03 | -0.25 | -0.18 | -0.27 | -0.22 | 0.46 | 0.52 | 0.32 | 2.08 | – | 1.51 | 0.41 | 0.42 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1859 | 0.405 | 0.3555 | 0.1387 | -0.091 | 0.4473 | 1.9423 | 1.1231 | 1.1997 | 0.8419 | 1.2079 | 0.9883 | 0.3274 | 0.2492 | 0.3399 | -0.2065 | -0.2707 | 0.1064 |
Actin | 0.0008 | 0.0132 | 0.0009 | 0.0007 | 0.001 | 0.0019 | 0.058 | 0.011 | 0.0257 | 0.0314 | 0.0297 | 0.0078 | 0.1011 | 0.0025 | 0.0115 | 0.0001 | 0.0002 | 0.0006 |
Bud | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0016 | 0.0003 | 0.0025 | 0.002 | 0.0011 | 0.0007 | 0.0027 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0007 | 0.0012 | 0.0066 | 0.0004 | 0.0102 | 0.0029 | 0.0003 | 0.0047 | 0.0011 | 0.0008 | 0.0101 | 0.0031 | 0.0012 | 0.0049 | 0.0001 | 0.0013 | 0.0088 |
Bud Periphery | 0 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0007 | 0.0025 | 0.0006 | 0.0036 | 0.002 | 0.0015 | 0.0013 | 0.0128 | 0.0005 | 0.0008 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0003 | 0.0073 | 0.0075 | 0.0006 | 0.0001 | 0.0007 | 0.0216 | 0.0018 | 0.0084 | 0.0041 | 0.0036 | 0.0004 | 0.0172 | 0.0023 | 0.0015 | 0.0002 | 0.0004 | 0.0001 |
Cell Periphery | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0015 | 0.0004 | 0.0007 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.1412 | 0.2465 | 0.1744 | 0.1925 | 0.2539 | 0.3163 | 0.0677 | 0.2307 | 0.1707 | 0.312 | 0.2592 | 0.3048 | 0.0604 | 0.1241 | 0.0621 | 0.0707 | 0.0959 | 0.0887 |
Cytoplasmic Foci | 0.2882 | 0.3178 | 0.3316 | 0.2083 | 0.3285 | 0.2659 | 0.2194 | 0.2953 | 0.3196 | 0.2145 | 0.1566 | 0.1983 | 0.203 | 0.1627 | 0.1618 | 0.2385 | 0.3472 | 0.2689 |
Eisosomes | 0.0003 | 0.0003 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0006 | 0.0018 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0029 | 0.0032 | 0.0048 | 0.0045 | 0.0022 | 0.0027 | 0.0137 | 0.0034 | 0.0023 | 0.0021 | 0.0016 | 0.0022 | 0.0042 | 0.0055 | 0.002 | 0.0009 | 0.0011 | 0.0007 |
Endosome | 0.0763 | 0.069 | 0.0756 | 0.0572 | 0.1163 | 0.0471 | 0.1063 | 0.0926 | 0.0804 | 0.0899 | 0.1637 | 0.0691 | 0.0328 | 0.0221 | 0.023 | 0.1554 | 0.0588 | 0.0248 |
Golgi | 0.0034 | 0.0043 | 0.0042 | 0.0021 | 0.0042 | 0.0024 | 0.0247 | 0.0039 | 0.0142 | 0.007 | 0.0571 | 0.0094 | 0.006 | 0.002 | 0.0023 | 0.0024 | 0.0028 | 0.0014 |
Lipid Particles | 0.0181 | 0.0156 | 0.0263 | 0.0121 | 0.0093 | 0.0109 | 0.046 | 0.0101 | 0.0174 | 0.0484 | 0.0324 | 0.0138 | 0.0261 | 0.0135 | 0.0255 | 0.0114 | 0.0168 | 0.0177 |
Mitochondria | 0.0006 | 0.0008 | 0.0011 | 0.0006 | 0.0004 | 0.0011 | 0.0217 | 0.0006 | 0.0055 | 0.0115 | 0.0665 | 0.0097 | 0.0052 | 0.0015 | 0.0011 | 0.0017 | 0.0082 | 0.0008 |
None | 0.45 | 0.2827 | 0.3269 | 0.4954 | 0.2384 | 0.315 | 0.2694 | 0.333 | 0.2761 | 0.2007 | 0.2067 | 0.3417 | 0.4922 | 0.6276 | 0.6792 | 0.4823 | 0.447 | 0.5724 |
Nuclear Periphery | 0.0005 | 0.0009 | 0.001 | 0.0007 | 0.0004 | 0.0004 | 0.0324 | 0.0015 | 0.0041 | 0.0029 | 0.0007 | 0.0011 | 0.0019 | 0.0016 | 0.0007 | 0.0009 | 0.0005 | 0.0002 |
Nucleolus | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0004 | 0.0031 | 0.0003 | 0.0006 | 0.0024 | 0.0003 | 0.0002 | 0.004 | 0.001 | 0.001 | 0.0003 | 0.0005 | 0.0002 |
Nucleus | 0.0005 | 0.001 | 0.0008 | 0.0007 | 0.0007 | 0.0007 | 0.0188 | 0.001 | 0.0019 | 0.0224 | 0.0005 | 0.0007 | 0.0014 | 0.0009 | 0.0008 | 0.0007 | 0.0004 | 0.0003 |
Peroxisomes | 0.0103 | 0.015 | 0.0265 | 0.0098 | 0.0394 | 0.0153 | 0.0292 | 0.0062 | 0.027 | 0.0345 | 0.01 | 0.0215 | 0.0139 | 0.0214 | 0.0133 | 0.0177 | 0.0117 | 0.0071 |
Punctate Nuclear | 0.0036 | 0.0165 | 0.0131 | 0.0024 | 0.0014 | 0.0036 | 0.0379 | 0.0034 | 0.0252 | 0.0025 | 0.0014 | 0.0027 | 0.0029 | 0.0053 | 0.0032 | 0.0014 | 0.0021 | 0.0054 |
Vacuole | 0.0021 | 0.0035 | 0.0025 | 0.004 | 0.0027 | 0.0035 | 0.0144 | 0.0032 | 0.0086 | 0.0066 | 0.0044 | 0.0034 | 0.0057 | 0.0028 | 0.0022 | 0.013 | 0.0043 | 0.0016 |
Vacuole Periphery | 0.0003 | 0.0004 | 0.0003 | 0.0007 | 0.0004 | 0.0004 | 0.0076 | 0.0004 | 0.001 | 0.0014 | 0.0018 | 0.0009 | 0.0009 | 0.0003 | 0.0002 | 0.0019 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.643 | 6.9718 | 7.4497 | 9.6132 | 6.6738 | 12.1597 | 8.9525 | 10.6574 | 8.1956 | 11.1454 |
Translational Efficiency | 0.7879 | 0.7856 | 0.5198 | 0.4966 | 0.6541 | 0.4632 | 0.6462 | 0.4623 | 0.658 | 0.4533 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1404 | 19 | 117 | 164 | 1833 | 1872 | 112 | 376 | 3237 | 1891 | 229 | 540 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 574.61 | 334.57 | 1087.85 | 902.24 | 671.10 | 710.05 | 959.32 | 826.37 | 629.25 | 706.28 | 1024.99 | 849.41 |
Standard Deviation | 87.14 | 18.37 | 98.49 | 100.13 | 76.37 | 96.95 | 95.37 | 125.59 | 94.25 | 103.49 | 116.33 | 123.47 |
Intensity Change Log 2 | — | -0.780275 | 0.920825 | 0.650928 | — | 0.081393 | 0.515484 | 0.300260 | — | -0.253990 | 0.716663 | 0.472644 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000210 | 0.000020 | 0.002537 | 0.005642 | 0.000353 | 0.001000 | 0.000830 | 0.002115 | 0.000291 | 0.000990 | 0.001703 | 0.003186 |
Bud Neck | 0.034673 | 0.000010 | 0.009552 | 0.023411 | 0.038696 | 0.051593 | 0.017423 | 0.016393 | 0.036951 | 0.051074 | 0.013401 | 0.018525 |
Bud Site | 0.007368 | 0.000016 | 0.026300 | 0.150138 | 0.006186 | 0.021457 | 0.010660 | 0.041283 | 0.006699 | 0.021241 | 0.018651 | 0.074343 |
Cell Periphery | 0.000276 | 0.000055 | 0.000439 | 0.000561 | 0.000084 | 0.000226 | 0.000283 | 0.000191 | 0.000167 | 0.000224 | 0.000363 | 0.000304 |
Cytoplasm | 0.330868 | 0.000034 | 0.166660 | 0.203744 | 0.309565 | 0.244174 | 0.169872 | 0.325451 | 0.318805 | 0.241721 | 0.168231 | 0.288488 |
Cytoplasmic Foci | 0.338264 | 0.000016 | 0.071844 | 0.104221 | 0.377780 | 0.289277 | 0.105045 | 0.176327 | 0.360641 | 0.286371 | 0.088082 | 0.154428 |
Eisosomes | 0.000237 | 0.000049 | 0.000188 | 0.000122 | 0.000182 | 0.000200 | 0.000206 | 0.000102 | 0.000206 | 0.000198 | 0.000197 | 0.000108 |
Endoplasmic Reticulum | 0.000539 | 0.000001 | 0.018703 | 0.003624 | 0.000671 | 0.000745 | 0.004807 | 0.002133 | 0.000614 | 0.000737 | 0.011907 | 0.002586 |
Endosome | 0.045295 | 0.000002 | 0.029658 | 0.058084 | 0.054804 | 0.103268 | 0.040524 | 0.038658 | 0.050680 | 0.102231 | 0.034972 | 0.044558 |
Golgi | 0.004555 | 0.000006 | 0.000619 | 0.013709 | 0.007107 | 0.015583 | 0.000610 | 0.002258 | 0.006001 | 0.015427 | 0.000615 | 0.005736 |
Lipid Particles | 0.018188 | 0.000044 | 0.008389 | 0.003486 | 0.015375 | 0.017385 | 0.006734 | 0.003767 | 0.016595 | 0.017211 | 0.007580 | 0.003682 |
Mitochondria | 0.005151 | 0.000010 | 0.000440 | 0.043349 | 0.007752 | 0.021856 | 0.000346 | 0.000448 | 0.006624 | 0.021636 | 0.000394 | 0.013477 |
Mitotic Spindle | 0.000753 | 0.000483 | 0.022660 | 0.035551 | 0.004273 | 0.013487 | 0.001228 | 0.034413 | 0.002746 | 0.013356 | 0.012178 | 0.034758 |
None | 0.013307 | 0.999056 | 0.000967 | 0.001001 | 0.001396 | 0.001609 | 0.001857 | 0.001409 | 0.006562 | 0.011631 | 0.001402 | 0.001285 |
Nuclear Periphery | 0.000422 | 0.000000 | 0.003741 | 0.000849 | 0.000344 | 0.000504 | 0.001471 | 0.001718 | 0.000377 | 0.000499 | 0.002631 | 0.001454 |
Nuclear Periphery Foci | 0.000664 | 0.000073 | 0.026362 | 0.005917 | 0.000410 | 0.000353 | 0.006149 | 0.004227 | 0.000520 | 0.000350 | 0.016476 | 0.004740 |
Nucleolus | 0.004931 | 0.000046 | 0.001494 | 0.001445 | 0.001508 | 0.002537 | 0.007474 | 0.000938 | 0.002992 | 0.002512 | 0.004419 | 0.001092 |
Nucleus | 0.110356 | 0.000012 | 0.267151 | 0.039897 | 0.081627 | 0.063475 | 0.467068 | 0.092983 | 0.094088 | 0.062837 | 0.364927 | 0.076861 |
Peroxisomes | 0.030413 | 0.000065 | 0.028682 | 0.032398 | 0.044701 | 0.039438 | 0.028243 | 0.087133 | 0.038503 | 0.039043 | 0.028468 | 0.070510 |
Vacuole | 0.052636 | 0.000001 | 0.313146 | 0.270138 | 0.046344 | 0.107421 | 0.128887 | 0.167860 | 0.049073 | 0.106342 | 0.223028 | 0.198923 |
Vacuole Periphery | 0.000894 | 0.000000 | 0.000466 | 0.002713 | 0.000841 | 0.004413 | 0.000281 | 0.000192 | 0.000864 | 0.004369 | 0.000376 | 0.000958 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 6.68 | -6.93 | -2.80 | -3.08 | 0.11 | -2.16 | -2.59 | -3.16 | -1.83 | -1.55 | -2.38 | -6.52 | -4.13 | -2.18 | -0.96 |
Bud Neck | 15.73 | 8.73 | 3.63 | -4.32 | -1.54 | -3.98 | 3.76 | 6.84 | 10.28 | -0.33 | -5.00 | 7.13 | 7.66 | 10.72 | -1.24 |
Bud Site | 7.79 | -3.46 | -4.17 | -4.75 | -2.97 | -7.51 | -1.80 | -5.80 | -2.28 | -3.47 | -7.33 | -3.82 | -6.82 | -3.72 | -4.26 |
Cell Periphery | 2.48 | -1.35 | 0.33 | -3.28 | 2.44 | -3.24 | -6.73 | -5.62 | 1.34 | 3.86 | -1.06 | -4.10 | -0.52 | 0.81 | 5.16 |
Cytoplasm | 43.59 | 12.01 | 7.21 | -9.63 | -1.81 | 6.97 | 8.23 | 3.00 | -1.02 | -4.73 | 9.35 | 14.02 | 6.32 | 0.50 | -5.66 |
Cytoplasmic Foci | 48.86 | 26.42 | 22.12 | -8.53 | -2.78 | 11.29 | 17.91 | 21.67 | 14.16 | -2.12 | 11.23 | 30.12 | 28.45 | 18.68 | -4.31 |
Eisosomes | 9.42 | 2.32 | 6.67 | -4.30 | 4.51 | -1.56 | -2.06 | 4.67 | 5.38 | 4.68 | 0.35 | -0.13 | 7.65 | 6.12 | 6.17 |
Endoplasmic Reticulum | 5.67 | -11.21 | -5.81 | -6.61 | 8.41 | -0.73 | -5.15 | -6.88 | -6.82 | 3.39 | -1.50 | -11.31 | -9.04 | -8.64 | 8.77 |
Endosome | 17.85 | 4.76 | 2.35 | -5.05 | -0.56 | -10.04 | 1.14 | 5.51 | 13.98 | 1.35 | -11.95 | 3.03 | 5.72 | 14.72 | 0.81 |
Golgi | 10.09 | 8.82 | 0.83 | -2.35 | -1.97 | -6.19 | 8.75 | 6.76 | 11.29 | -0.82 | -7.53 | 11.77 | 5.81 | 10.53 | -2.09 |
Lipid Particles | 14.42 | 6.78 | 9.41 | -10.70 | 3.45 | -1.39 | 3.77 | 8.69 | 11.34 | 2.99 | -0.60 | 7.21 | 12.74 | 11.56 | 4.45 |
Mitochondria | 7.64 | 6.85 | -0.89 | -1.85 | -1.75 | -7.60 | 7.63 | 7.56 | 13.32 | -0.50 | -8.85 | 9.95 | 1.84 | 8.00 | -1.75 |
Mitotic Spindle | 1.29 | -1.62 | -3.58 | -3.62 | -1.83 | -4.42 | -0.72 | -5.05 | -3.83 | -2.97 | -5.53 | -1.62 | -6.25 | -4.44 | -3.52 |
None | -487.87 | 6.39 | 5.06 | 1029.31 | -1.82 | -0.88 | -0.74 | -1.61 | -0.61 | -0.08 | -2.01 | 5.69 | 4.86 | 4.10 | -1.57 |
Nuclear Periphery | 11.02 | -9.46 | -8.13 | -9.96 | 4.85 | -2.57 | -6.25 | -3.22 | -2.99 | -0.61 | -2.21 | -10.78 | -4.37 | -4.11 | 1.84 |
Nuclear Periphery Foci | 9.40 | -5.46 | -4.63 | -4.93 | 3.46 | 1.44 | -3.13 | -6.24 | -6.30 | 1.32 | 3.89 | -6.17 | -7.74 | -7.92 | 3.64 |
Nucleolus | 9.73 | 5.69 | 3.83 | -2.91 | -0.52 | -2.91 | -2.08 | -1.10 | 0.45 | 1.76 | 2.71 | -0.41 | 2.45 | 0.48 | 1.46 |
Nucleus | 25.75 | -7.05 | 5.02 | -9.21 | 8.55 | 4.30 | -9.27 | -1.92 | -3.95 | 8.57 | 8.10 | -11.57 | 0.71 | -3.73 | 11.39 |
Peroxisomes | 15.91 | 2.86 | 0.67 | -4.00 | -1.35 | 1.56 | 2.33 | -0.12 | -0.87 | -1.97 | -0.21 | 3.77 | -0.18 | -0.06 | -2.90 |
Vacuole | 20.68 | -15.15 | -16.27 | -18.48 | -2.21 | -14.74 | -7.08 | -17.24 | -12.96 | -4.08 | -14.41 | -14.82 | -22.86 | -18.03 | -2.77 |
Vacuole Periphery | 9.93 | 4.24 | 0.38 | -2.35 | -0.85 | -11.60 | 4.94 | 7.88 | 14.22 | 2.51 | -11.70 | 6.46 | 4.36 | 12.94 | 0.52 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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