Standard name
Human Ortholog
Description Probable Hsp40p co-chaperone; has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0.06 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.08 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – 0.08 0.08 0 0 0 0 0 0
Cytoplasm 0.1 0.24 0.26 0.16 0.11 0.1 0.11 0.07 0 0.05 0.05 0 0.29 0.41 0.54 – 0.25 0.16 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0
Endosome 0 0 0.14 0.1 0.23 0.05 0 0.07 0 0 0 0 0.15 0.13 0.12 – 0.16 0.15 0.07 0.05 0.06 0.05 0.05 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0.09 0 0 0 0 0 0
Mitochondria 0.8 0.77 0.28 0.71 0.58 0.77 0.89 0.73 0.94 0.92 0.92 0.94 0.34 0.33 0.18 – 0.14 0.13 0.76 0.73 0.78 0.65 0.7 0.63
Nucleus 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0.07 0.06 0 0 0 0 0 0 0 0.07 0.15 0.11 – 0.08 0 0 0 0 0 0 0.05
SpindlePole 0 0 0.22 0.05 0.11 0 0 0.12 0 0 0.06 0.06 0.05 0.05 0 – 0.09 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.05 0.07 0.06 0.13 0.11 0 0.08 0 0.05 0 0.06 0.11 0.08 0.07 – 0.16 0.2 0 0.06 0 0.06 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 6 3 0 0 0 0 0 0 0 0 7 0 1 – 4 4 3 8 7 5 2 5
Bud 0 14 2 7 6 5 10 7 15 9 20 18 4 3 2 – 5 7 0 1 0 0 1 5
Bud Neck 0 2 4 0 0 1 0 2 1 1 0 1 2 1 2 – 2 0 1 5 1 1 1 1
Bud Site 1 3 0 1 0 5 4 2 3 4 4 9 0 0 0 – 0 0 – – – – – –
Cell Periphery 4 4 9 1 3 2 1 2 1 4 0 6 2 5 3 – 5 8 1 0 0 0 0 0
Cytoplasm 17 85 41 27 27 26 29 25 15 19 22 13 38 89 124 – 16 15 3 22 2 4 7 7
Endoplasmic Reticulum 11 13 0 1 1 5 5 3 5 2 2 1 3 1 4 – 3 4 0 5 0 5 2 2
Endosome 7 0 22 17 56 12 0 23 0 1 3 1 20 29 28 – 10 14 15 19 14 10 8 4
Golgi 1 0 2 1 2 0 0 2 0 0 1 0 1 0 4 – 3 9 3 1 1 4 2 2
Mitochondria 139 270 45 121 143 200 239 254 372 336 413 497 44 73 42 – 9 12 174 304 199 136 125 114
Nucleus 0 1 4 0 4 7 14 16 9 13 9 7 1 0 0 – 1 5 0 0 0 1 4 1
Nuclear Periphery 1 0 0 1 0 1 1 3 15 6 12 5 0 0 0 – 2 1 2 2 2 2 2 2
Nucleolus 0 0 0 1 0 1 1 4 5 3 13 4 0 0 0 – 0 1 1 0 0 2 0 1
Peroxisomes 1 0 11 12 14 3 0 5 2 1 1 3 9 33 25 – 5 4 1 5 9 9 4 8
SpindlePole 7 5 34 9 26 9 7 41 12 15 29 30 6 10 8 – 6 10 4 3 4 4 3 7
Vac/Vac Membrane 20 18 11 11 32 30 5 27 16 20 17 32 14 17 16 – 10 19 9 25 3 13 8 9
Unique Cell Count 174 351 158 170 247 261 269 350 394 367 450 528 130 218 228 64 95 228 418 255 210 180 183
Labelled Cell Count 212 419 191 213 314 307 316 416 471 434 546 627 151 261 259 81 113 228 418 255 210 180 183


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 3.4 5.4 5.1 5.7 4.9 3.4 5.1 4.1 4.4 4.2 4.2 5.1 5.1 5.2 4.7 5.9 6.2 6.1 6.6 6.5
Std Deviation (1e-4) 0.5 0.5 1.3 2.1 1.8 1.6 0.9 1.7 1.2 1.2 1.1 0.8 1.1 1.0 1.5 1.0 1.5 1.5 2.0 2.4 2.4
Intensity Change (Log2) – – – -0.07 0.07 -0.13 -0.66 -0.08 -0.39 -0.29 -0.34 -0.35 -0.08 -0.06 -0.04 -0.21 0.13 0.2 0.18 0.29 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 2.0 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2084 4.8461 4.8072 4.4091 5.2465 4.4739 4.4927 4.1477 4.6514 4.2623 4.568 4.6958 2.7148 2.1877 2.06 2.1482 2.0451 2.0424
Actin 0.0606 0.0083 0.0144 0.0133 0.0797 0.0296 0.0321 0.0061 0.0298 0.0219 0.0212 0.019 0.065 0.0168 0.0282 0.0304 0.0036 0.0136
Bud 0.0023 0.0041 0.0033 0.0017 0.0031 0.0006 0.0014 0.0018 0.0027 0.0117 0.0048 0.0008 0.0013 0.0026 0.0028 0.0018 0.0006 0.0007
Bud Neck 0.0019 0.0039 0.0075 0.0149 0.0026 0.0063 0.0015 0.0013 0.0011 0.0008 0.0167 0.0026 0.0053 0.0024 0.002 0.0032 0.0005 0.0089
Bud Periphery 0.0056 0.0052 0.0067 0.0075 0.0076 0.0015 0.0034 0.0037 0.0109 0.0154 0.0245 0.0031 0.0026 0.0046 0.0045 0.0086 0.002 0.0029
Bud Site 0.0184 0.0907 0.0311 0.0042 0.0021 0.0023 0.0056 0.0295 0.0108 0.005 0.005 0.0027 0.0104 0.0562 0.024 0.0072 0.0017 0.0012
Cell Periphery 0.002 0.0032 0.0049 0.0072 0.0016 0.0012 0.0031 0.001 0.0016 0.0005 0.0012 0.0017 0.0026 0.0067 0.0032 0.0043 0.0011 0.0017
Cytoplasm 0.0353 0.0091 0.0221 0.0015 0.0257 0.0149 0.0085 0.0062 0.008 0.0054 0.0148 0.0089 0.0155 0.0363 0.027 0.022 0.0679 0.0173
Cytoplasmic Foci 0.0695 0.0765 0.0573 0.013 0.0223 0.0453 0.0565 0.0442 0.047 0.0359 0.0725 0.0456 0.1076 0.0736 0.0631 0.0431 0.0291 0.0537
Eisosomes 0.0012 0.0035 0.0023 0.001 0.0009 0.0011 0.0006 0.0003 0.0008 0.0002 0.0009 0.0009 0.001 0.0039 0.0028 0.0006 0.0003 0.0006
Endoplasmic Reticulum 0.0265 0.0012 0.0056 0.0003 0.0187 0.002 0.0036 0.0005 0.001 0.0006 0.005 0.001 0.0076 0.0024 0.0042 0.0018 0.0026 0.0009
Endosome 0.075 0.0803 0.0659 0.0188 0.0677 0.0648 0.1297 0.0328 0.0531 0.0743 0.1153 0.0488 0.1509 0.1474 0.1277 0.0822 0.0611 0.065
Golgi 0.0498 0.0794 0.0479 0.0376 0.063 0.0602 0.0819 0.0459 0.0413 0.036 0.0453 0.0636 0.0734 0.0872 0.0697 0.0539 0.0431 0.0669
Lipid Particles 0.0354 0.0092 0.0163 0.002 0.0064 0.0411 0.0373 0.0051 0.0174 0.0383 0.0441 0.0201 0.0513 0.0188 0.0121 0.0138 0.0255 0.0378
Mitochondria 0.471 0.5096 0.617 0.8582 0.5301 0.6503 0.4989 0.701 0.6795 0.6937 0.4738 0.6653 0.3515 0.4194 0.5322 0.6468 0.6249 0.6394
None 0.0308 0.0517 0.0271 0.0013 0.0456 0.0114 0.0087 0.0147 0.0278 0.0008 0.0135 0.0266 0.0215 0.0182 0.0163 0.0128 0.043 0.0305
Nuclear Periphery 0.0198 0.0003 0.0073 0.0001 0.0034 0.0005 0.0126 0.0001 0.0005 0.0002 0.0119 0.0003 0.012 0.0009 0.0048 0.0005 0.0075 0.0003
Nucleolus 0.0013 0.0056 0.0013 0.0011 0.0004 0.0003 0.0021 0.0008 0.0018 0.0003 0.0006 0.0007 0.0118 0.0007 0.0008 0.0002 0.0004 0.0003
Nucleus 0.0068 0.0017 0.0062 0.0005 0.0015 0.0004 0.0018 0.0006 0.0012 0.0004 0.0044 0.0005 0.0206 0.0008 0.0087 0.0005 0.0336 0.0005
Peroxisomes 0.0531 0.041 0.0378 0.0112 0.0966 0.0523 0.074 0.1001 0.0513 0.05 0.109 0.0776 0.0584 0.0794 0.0518 0.0573 0.0433 0.0503
Punctate Nuclear 0.0206 0.0008 0.0017 0.0001 0.0007 0.0006 0.012 0.0004 0.0011 0.0002 0.0017 0.0004 0.0119 0.0005 0.0008 0.0006 0.0009 0.0008
Vacuole 0.0085 0.0121 0.0127 0.0029 0.009 0.0053 0.0182 0.0023 0.0063 0.0056 0.0084 0.0079 0.0126 0.0122 0.0091 0.0046 0.0034 0.0039
Vacuole Periphery 0.0048 0.0027 0.0035 0.0015 0.0115 0.0079 0.0064 0.0016 0.0048 0.0028 0.0053 0.002 0.0049 0.009 0.0041 0.0038 0.0038 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8715 2.1774 3.2315 5.3614 0.7895 7.3033 5.2288 5.2008 6.3991 4.4677
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1765 1491 206 193 2128 1341 1751 2072 3893 2832 1957 2265

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 728.27 767.24 896.58 930.26 675.22 824.41 813.21 883.75 699.27 794.31 821.99 887.71
Standard Deviation 78.07 100.44 110.56 111.12 81.26 104.48 108.49 117.49 84.09 106.28 111.68 117.68
Intensity Change Log 2 — 0.075205 0.299959 0.353161 — 0.288004 0.268270 0.388281 — 0.181504 0.284800 0.370164

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000776 0.002739 0.005155 0.018020 0.000465 0.000832 0.000916 0.010559 0.000606 0.001836 0.001362 0.011195
Bud Neck 0.017710 0.021629 0.026946 0.028214 0.020092 0.030644 0.049027 0.026258 0.019012 0.025898 0.046702 0.026425
Bud Site 0.011703 0.046126 0.042374 0.159676* 0.015181 0.019442 0.028119 0.105498* 0.013604 0.033491 0.029619 0.110115*
Cell Periphery 0.000203 0.000459 0.000392 0.000519 0.000263 0.000171 0.000373 0.000295 0.000236 0.000323 0.000375 0.000314
Cytoplasm 0.036785 0.030063 0.120842* 0.049752 0.050133 0.039940 0.096105 0.092713 0.044081 0.034740 0.098709 0.089052
Cytoplasmic Foci 0.195183* 0.133189 0.140995 0.176414 0.221980* 0.147894 0.136431 0.148232 0.209831* 0.140152 0.136911 0.150634
Eisosomes 0.000265 0.000173 0.000261 0.000229 0.000200 0.000131 0.000183 0.000168 0.000230 0.000153 0.000191 0.000173
Endoplasmic Reticulum 0.002318 0.000667 0.013060 0.003272 0.001421 0.000435 0.004697 0.003531 0.001827 0.000557 0.005577 0.003509
Endosome 0.063445 0.086389 0.051748 0.075905 0.069966 0.093597 0.088315 0.071434 0.067010 0.089802 0.084465 0.071815
Golgi 0.108191 0.122162* 0.010475 0.074069 0.109836 0.098523 0.025268 0.070562 0.109090 0.110968* 0.023711 0.070861
Lipid Particles 0.014248 0.005250 0.024321 0.002091 0.016639 0.008048 0.030882 0.004517 0.015555 0.006575 0.030191 0.004310
Mitochondria 0.453789* 0.415048* 0.020219 0.051073 0.394555* 0.434941* 0.101934* 0.162834* 0.421410* 0.424467* 0.093333 0.153311*
Mitotic Spindle 0.004413 0.017914 0.033612 0.045637 0.005838 0.015434 0.058578 0.057055 0.005192 0.016739 0.055950 0.056082
None 0.000711 0.000842 0.001529 0.000198 0.001261 0.001026 0.007699 0.000793 0.001012 0.000929 0.007049 0.000742
Nuclear Periphery 0.000324 0.000213 0.003151 0.000700 0.000289 0.000124 0.003994 0.000877 0.000305 0.000171 0.003906 0.000862
Nuclear Periphery Foci 0.000204 0.000237 0.005176 0.001268 0.000232 0.000104 0.004195 0.000704 0.000219 0.000174 0.004298 0.000752
Nucleolus 0.000562 0.001547 0.010340 0.000598 0.001192 0.001456 0.024094 0.001230 0.000906 0.001504 0.022647 0.001176
Nucleus 0.007285 0.005744 0.263619* 0.008301 0.009472 0.006672 0.138764* 0.011790 0.008480 0.006183 0.151906* 0.011493
Peroxisomes 0.039088 0.063864 0.071242 0.212146* 0.038981 0.040281 0.046902 0.073186 0.039029 0.052697 0.049464 0.085026
Vacuole 0.029761 0.029413 0.147886 0.086272 0.026798 0.045281 0.136602 0.139182 0.028141 0.036927 0.137790 0.134674
Vacuole Periphery 0.013038 0.016333 0.006658 0.005645 0.015209 0.015024 0.016924 0.018581 0.014225 0.015713 0.015843 0.017479

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.01 -4.91 -6.08 -5.31 -4.33 -1.65 -6.48 -9.76 -9.19 -9.26 -4.43 -6.28 -10.84 -9.21 -9.95
Bud Neck -1.81 -2.21 -2.25 -1.34 -0.19 -3.99 -10.87 -3.59 1.33 8.13 -4.07 -11.90 -4.88 -0.62 7.85
Bud Site -11.52 -4.55 -11.10 -8.30 -8.06 -2.49 -6.34 -23.72 -21.77 -19.10 -11.09 -8.59 -26.64 -19.31 -20.51
Cell Periphery -5.20 -4.32 -2.31 -0.35 -0.91 2.85 -2.03 -0.54 -3.33 1.47 -2.82 -3.40 -1.96 0.46 1.21
Cytoplasm 2.14 -7.03 -2.44 -3.29 4.76 2.69 -10.54 -11.02 -12.54 0.17 3.80 -14.01 -13.46 -15.64 1.38
Cytoplasmic Foci 10.49 6.42 1.92 -3.41 -3.17 11.77 14.95 13.78 0.36 -1.78 16.18 15.80 13.56 -1.79 -2.59
Eisosomes 4.97 0.15 0.91 -2.34 0.75 6.10 1.27 2.74 -3.27 2.05 7.23 3.35 4.78 -1.88 2.32
Endoplasmic Reticulum 3.83 -7.33 -2.04 -4.07 5.61 4.66 -9.07 -4.43 -7.13 2.43 5.59 -10.00 -3.85 -7.55 4.40
Endosome -5.52 3.18 -1.37 1.71 -3.26 -5.24 -3.97 0.17 5.48 4.25 -7.46 -4.35 -0.98 6.16 3.29
Golgi -2.44 24.57 3.89 5.12 -6.12 2.12 22.49 9.51 6.38 -13.70 -0.46 30.03 11.33 10.94 -15.01
Lipid Particles 6.63 -2.32 9.39 2.90 5.96 5.79 -6.26 9.93 3.27 13.28 8.95 -7.36 12.24 2.97 14.14
Mitochondria 3.37 46.74* 30.91* 27.14* -2.62 -3.54 36.46* 27.19 26.74 -9.13 -0.36 51.25* 38.16* 35.15* -9.67
Mitotic Spindle -8.57 -4.02 -5.59 -3.69 -1.13 -4.60 -15.02 -16.30 -11.39 0.37 -8.93 -15.76 -17.42 -12.61 0.02
None -0.37 -1.70 1.37 2.31 3.93 0.85 -5.85 1.11 0.43 6.43 0.48 -6.31 0.70 0.37 6.52
Nuclear Periphery 1.10 -7.51 -0.94 -1.14 3.75 3.55 -9.70 -3.66 -4.80 7.52 2.42 -10.51 -3.70 -4.55 8.09
Nuclear Periphery Foci -0.53 -5.61 -2.97 -2.93 2.64 4.95 -8.58 -4.67 -6.21 7.50 1.44 -9.66 -5.58 -6.11 8.05
Nucleolus -2.35 -3.86 -0.79 1.65 3.75 -0.48 -14.61 -0.20 0.40 14.67 -1.84 -15.25 -1.65 0.82 15.05
Nucleus 1.00 -12.90 -1.91 -2.25 12.41 1.97 -24.90 -2.69 -4.79 24.20 2.20 -27.94 -4.21 -5.94 26.77
Peroxisomes -5.93 -2.47 -8.62 -7.21 -6.58 -0.39 -2.59 -8.83 -7.48 -6.44 -5.09 -3.50 -11.80 -7.28 -8.42
Vacuole -0.04 -11.33 -6.51 -6.49 4.07 -6.19 -24.33 -25.37 -19.37 -1.41 -4.66 -26.55 -26.12 -23.27 -0.35
Vacuole Periphery -2.56 4.52 6.25 7.34 0.17 0.14 -0.34 -2.03 -2.06 -1.64 -1.64 -0.34 -2.38 -0.88 -1.78
Standard name
Human Ortholog
Description Probable Hsp40p co-chaperone; has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
Localization
Cell Percentages mitochondrion (92%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Jid1

Jid1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Jid1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available