Standard name
Human Ortholog
Description Cytosine deaminase; zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.96 0.96 0.93 – 0.74 0.75 0.66 0.72 0.63 0.98 0.99 0.99 0.94 0.93 0.91 0.97 0.96 0.95 0.96 0.95 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 – 0 0.14 0.21 0.23 0.27 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.2 0.1 0.12 0.05 0.1 0 0 0 0.06 0.06 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 – 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2
Bud 1 0 1 0 0 0 – 2 0 1 0 3 0 0 1 0 0 0 2 0 0 1 2 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 2 1 3 3 – 6 13 7 9 11 0 1 3 0 0 0 0 0 0 0 0 0
Cytoplasm 529 174 305 179 287 368 – 264 266 224 217 216 509 642 597 78 105 59 502 167 287 403 460 483
Endoplasmic Reticulum 1 0 0 0 5 3 – 10 12 15 3 7 1 5 2 0 0 0 1 0 0 3 2 5
Endosome 0 0 0 0 0 0 – 0 0 2 0 0 0 1 0 0 0 0 0 0 0 0 1 3
Golgi 0 0 0 0 0 0 – 2 1 0 0 0 1 3 2 0 0 0 0 0 0 0 2 4
Mitochondria 1 1 0 3 1 18 – 7 50 71 71 93 2 0 0 0 1 1 0 0 0 1 1 1
Nucleus 4 3 4 1 0 1 – 7 4 3 5 9 0 1 0 3 0 1 2 2 3 3 5 3
Nuclear Periphery 0 0 1 0 0 0 – 2 2 2 2 1 0 0 0 0 0 0 0 0 0 0 2 1
Nucleolus 0 0 0 0 0 1 – 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 0 3 3 8 10 – 70 37 42 15 35 10 2 3 5 7 6 3 1 1 3 4 3
Unique Cell Count 533 176 310 186 300 394 357 353 340 303 345 519 650 605 83 113 65 518 175 302 422 486 516
Labelled Cell Count 542 179 316 187 304 406 371 387 367 322 375 523 655 611 86 113 67 518 175 302 422 486 516


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.2 25.2 13.8 18.7 18.2 14.8 – 12.0 10.6 10.0 9.6 9.1 22.6 22.5 21.0 31.4 27.0 31.7 20.8 21.1 20.9
Std Deviation (1e-4) 3.9 4.5 3.0 4.1 3.5 3.1 – 2.6 2.3 2.0 2.1 1.7 4.4 3.8 3.7 11.5 11.3 11.9 3.8 4.6 4.4
Intensity Change (Log2) – – – 0.43 0.4 0.1 – -0.21 -0.38 -0.46 -0.53 -0.6 0.71 0.7 0.6 1.18 0.96 1.2 0.59 0.61 0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 2.6 0 2.2 2.3 0 0 0 0 0 0
Cytoplasm -1.5 -2.0 -3.2 – -8.9 -8.6 -10.6 -9.3 -11.3 -0.3 0.5 0.4 -2.3 -2.9 -3.3
Endoplasmic Reticulum 0 0 0 – 0 3.3 3.7 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 – 0 6.9 8.5 9.1 9.9 0 0 0 0 0 0
Nucleus 0 0 0 – 0.7 0 0 0 1.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.6 1.5 – 7.7 5.1 5.7 2.9 5.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.1617 23.7114 20.8871 22.5672 19.3339 21.3043 16.9074 23.8615 19.6186 18.8105 18.3861 21.3934 20.7381 24.0108 22.9516 20.9863 15.4433 21.7191
Actin 0.0129 0.0001 0.0146 0.0008 0.0005 0.005 0.0168 0.0009 0.0168 0.0168 0.0015 0.0002 0.0102 0.0003 0.0001 0.0571 0.006 0.0002
Bud 0.0004 0 0.0003 0.0002 0.0001 0.0003 0.0012 0.0001 0.0011 0.0007 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0017 0.0001
Bud Neck 0.0003 0.0001 0.0007 0.0005 0.0003 0.0009 0.0008 0.0001 0.0006 0.0006 0.0004 0.0006 0.0004 0.0001 0.0002 0.0004 0.0008 0.0008
Bud Periphery 0.0002 0 0.0002 0.0004 0.0001 0.0002 0.0011 0 0.0007 0.0015 0 0.0001 0.0001 0.0001 0 0.0002 0.0013 0
Bud Site 0.0029 0 0.0024 0.0007 0.0002 0.0002 0.0022 0.0001 0.003 0.0003 0.0001 0.0001 0.0005 0.0001 0 0.0003 0.0013 0
Cell Periphery 0.0001 0 0.0001 0.0002 0 0 0.0003 0 0.0002 0.0001 0 0 0 0 0 0 0.0002 0
Cytoplasm 0.8591 0.9905 0.9151 0.9242 0.8777 0.9137 0.8405 0.9885 0.878 0.7937 0.9022 0.949 0.9348 0.981 0.9778 0.8857 0.6725 0.9574
Cytoplasmic Foci 0.0179 0.0002 0.0087 0.002 0.0172 0.0109 0.0151 0.001 0.0199 0.0087 0.0141 0.005 0.0028 0.0005 0.0003 0.0009 0.0366 0.0007
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0 0 0 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0037 0.0002 0.0014 0.0017 0.0014 0.0014 0.0091 0.0002 0.0071 0.0015 0.0019 0.0015 0.0027 0.0003 0.0003 0.0006 0.0133 0.001
Endosome 0.0309 0 0.0094 0.0134 0.0562 0.0129 0.0297 0.0001 0.0156 0.0241 0.0192 0.0047 0.0064 0.0009 0.0001 0.0015 0.043 0.0006
Golgi 0.011 0 0.0093 0.0021 0.0152 0.0075 0.0125 0 0.0055 0.0303 0.0283 0.0003 0.0029 0.0002 0 0.0029 0.0182 0
Lipid Particles 0.0047 0 0.0039 0.0089 0.002 0.0087 0.0066 0 0.0066 0.0009 0.0031 0.0005 0.001 0.0005 0 0.0011 0.0435 0
Mitochondria 0.0051 0 0.0035 0.0104 0.0046 0.0043 0.0106 0.0001 0.0146 0.059 0.0013 0.0047 0.0003 0.0058 0.0001 0.0005 0.0409 0.0001
None 0.0057 0.0002 0.0006 0.0005 0.0004 0.0004 0.0018 0.0005 0.0047 0.0002 0.0002 0.001 0.0007 0.0003 0.0003 0.0004 0.0279 0.0003
Nuclear Periphery 0.0088 0.0007 0.0018 0.0059 0.0034 0.005 0.0114 0.0007 0.0048 0.0029 0.0054 0.0042 0.0038 0.0008 0.002 0.0055 0.028 0.0033
Nucleolus 0.0003 0 0.0004 0.001 0 0.0001 0.0002 0 0.0008 0 0 0.0001 0 0.0001 0 0 0.0018 0
Nucleus 0.0265 0.0076 0.0121 0.0137 0.0176 0.0249 0.0266 0.0072 0.0123 0.0535 0.0104 0.0226 0.0303 0.0073 0.0182 0.0409 0.0216 0.0345
Peroxisomes 0.0013 0 0.0128 0.0005 0.0006 0.0014 0.0027 0.0001 0.0039 0.0013 0.0023 0.0001 0.0012 0.0001 0 0.0008 0.0153 0
Punctate Nuclear 0.0054 0.0001 0.0008 0.0006 0.0006 0.0008 0.0022 0.0004 0.0013 0.0004 0.0003 0.0007 0.0006 0.0001 0.0003 0.0006 0.0163 0.0004
Vacuole 0.0015 0.0001 0.0015 0.008 0.0014 0.0008 0.0068 0.0001 0.0017 0.0012 0.0078 0.0029 0.0008 0.0006 0.0001 0.0002 0.0065 0.0005
Vacuole Periphery 0.0011 0 0.0003 0.0041 0.0006 0.0005 0.0017 0 0.0008 0.0019 0.0014 0.0014 0.0002 0.0006 0 0.0001 0.0031 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 75.1795 68.2399 122.7562 139.5526 136.463 110.9729 110.8138 136.8832 171.6319 120.2765
Translational Efficiency 4.8306 6.0205 2.8395 2.4552 2.892 3.4755 3.4344 2.4894 2.0127 2.6793

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2397 4 1200 1185 1752 1 1828 1617 4149 5 3028 2802

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1208.76 1582.62 2125.04 2096.49 1309.67 1615.78 2174.54 2145.04 1251.37 1589.25 2154.92 2124.51
Standard Deviation 190.15 192.58 321.14 373.33 234.51 — 316.20 386.49 215.86 172.76 319.09 381.74
Intensity Change Log 2 — 0.388787 0.813962 0.794448 — 0.303027 0.731507 0.711801 — 0.344826 0.771671 0.752060

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000051 0.000446 0.000141 0.001088 0.000084 0.000020 0.000340 0.000682 0.000065 0.000361 0.000261 0.000854
Bud Neck 0.000306 0.000959 0.001133 0.004031 0.000217 0.000061 0.001232 0.003139 0.000269 0.000779 0.001193 0.003516
Bud Site 0.001048 0.053951 0.001132 0.015948 0.001101 0.000383 0.002581 0.010128 0.001070 0.043238 0.002007 0.012589
Cell Periphery 0.000024 0.004318 0.000137 0.000343 0.000031 0.000014 0.000104 0.000146 0.000027 0.003457 0.000117 0.000229
Cytoplasm 0.860716* 0.923408* 0.554698* 0.661433* 0.866242* 0.937909* 0.642181* 0.644054* 0.863049* 0.926308* 0.607512* 0.651404*
Cytoplasmic Foci 0.013764 0.001399 0.003637 0.006151 0.009413 0.001770 0.003137 0.010443 0.011927 0.001474 0.003335 0.008628
Eisosomes 0.000002 0.000011 0.000001 0.000006 0.000001 0.000001 0.000001 0.000005 0.000002 0.000009 0.000001 0.000005
Endoplasmic Reticulum 0.000204 0.000056 0.000094 0.000746 0.000305 0.000023 0.000068 0.000284 0.000246 0.000049 0.000079 0.000479
Endosome 0.000273 0.000108 0.000095 0.002101 0.000240 0.000055 0.000109 0.005560 0.000259 0.000098 0.000104 0.004098
Golgi 0.000201 0.000106 0.000205 0.007760 0.000353 0.000062 0.000165 0.014035 0.000265 0.000097 0.000181 0.011381
Lipid Particles 0.000111 0.000508 0.000262 0.001655 0.000155 0.000010 0.000361 0.001076 0.000129 0.000408 0.000322 0.001321
Mitochondria 0.000126 0.000033 0.000256 0.001786 0.000463 0.000006 0.000193 0.001537 0.000268 0.000027 0.000218 0.001642
Mitotic Spindle 0.000817 0.000230 0.001692 0.019438 0.000665 0.000030 0.006026 0.080394 0.000753 0.000190 0.004308 0.054615
None 0.012695 0.000810 0.012615 0.008272 0.006195 0.011112 0.014319 0.008902 0.009950 0.002870 0.013644 0.008636
Nuclear Periphery 0.000062 0.000016 0.000218 0.000229 0.000068 0.000024 0.000193 0.000419 0.000065 0.000018 0.000203 0.000338
Nuclear Periphery Foci 0.001281 0.000442 0.000666 0.002903 0.001680 0.003758 0.000774 0.004475 0.001450 0.001105 0.000731 0.003810
Nucleolus 0.000165 0.000041 0.000093 0.000194 0.000108 0.000072 0.000095 0.001761 0.000141 0.000047 0.000095 0.001098
Nucleus 0.098889 0.006508 0.418213* 0.235950* 0.102705 0.043298 0.320459* 0.179476* 0.100500 0.013866 0.359199* 0.203359*
Peroxisomes 0.000060 0.000102 0.000029 0.000326 0.000032 0.000004 0.000075 0.000241 0.000048 0.000082 0.000056 0.000277
Vacuole 0.009114 0.006443 0.004637 0.023729 0.009058 0.001377 0.007551 0.024834 0.009090 0.005430 0.006396 0.024367
Vacuole Periphery 0.000093 0.000106 0.000046 0.005913 0.000887 0.000012 0.000034 0.008411 0.000428 0.000087 0.000039 0.007354

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Bud Neck NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Bud Site NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cell Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cytoplasm NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Cytoplasmic Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Eisosomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Endoplasmic Reticulum NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Endosome NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Golgi NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Lipid Particles NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Mitochondria NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Mitotic Spindle NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
None NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nuclear Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nuclear Periphery Foci NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nucleolus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Nucleus NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Peroxisomes NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Vacuole NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Vacuole Periphery NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN NaN
Standard name
Human Ortholog
Description Cytosine deaminase; zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Fcy1

Fcy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fcy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available