Standard name
Human Ortholog
Description Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.08 0.11 0.11 0.07 0.05 0.06 0 0 0.05 0.06 0.1 0.16 0.11 0.07 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0 0 0 0 0.16 0.32 0.16 0.37 0.34 0.4 0.24 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.93 0.95 0.9 0.9 0.84 0.82 0.84 0.83 0.76 0.78 0.78 0.76 0.92 0.87 0.81 0.8 0.81 0.82 0.88 0.92 0.86 0.84 0.75 0.68
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.06 0.07 0.05 0 0 0.11 0.12 0.16 0.18 0.21 0.22 0 0 0 0 0 0 0 0 0 0.06 0.09 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0 0 0 0 0 0.11 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Bud 0 0 0 0 1 0 1 1 3 2 3 1 0 0 0 1 1 0 1 0 1 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 1
Bud Site 1 0 1 0 0 1 4 1 7 6 13 10 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 2 6 14 24 19 22 12 13 8 10 14 16 25 46 38 22 23 0 0 0 1 2 1
Endoplasmic Reticulum 1 0 1 0 0 1 0 0 0 0 1 0 0 0 1 9 6 7 0 0 0 0 1 1
Endosome 1 0 1 0 6 0 0 0 1 0 0 3 0 1 2 5 10 2 2 1 1 1 0 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 1 2 2 2 0 0 0
Mitochondria 31 5 0 2 8 29 100 40 80 84 103 60 2 1 0 13 6 18 7 0 2 2 3 7
Nucleus 278 169 113 151 178 145 265 202 164 193 201 195 230 224 235 264 239 211 268 171 116 181 192 185
Nuclear Periphery 0 1 0 0 3 0 0 0 0 1 2 0 0 0 0 1 6 0 1 0 0 0 0 0
Nucleolus 32 11 9 9 3 6 35 29 34 46 54 56 1 6 11 8 10 9 11 3 3 13 23 22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 1 3 0 0 0 0 1 3 3 6 2 1 2 0 1 0 1 5
Vac/Vac Membrane 1 2 2 0 5 5 1 3 0 3 0 4 6 2 2 20 20 11 3 4 3 8 27 37
Unique Cell Count 299 177 125 167 211 177 317 243 216 249 259 255 251 258 291 332 296 258 306 186 135 216 257 275
Labelled Cell Count 353 190 133 177 228 207 429 291 302 343 387 344 256 263 303 365 328 283 306 186 135 216 257 275


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 8.5 8.2 7.7 7.2 5.8 5.4 6.1 5.2 5.2 5.2 5.8 9.5 9.0 8.7 7.9 8.8 7.9 7.4 7.4 7.3
Std Deviation (1e-4) 1.2 1.5 1.7 2.2 2.4 1.8 1.5 1.8 1.3 1.4 1.4 1.5 2.3 2.0 2.4 1.8 2.2 2.0 1.4 1.2 1.7
Intensity Change (Log2) – – – -0.09 -0.19 -0.5 -0.62 -0.42 -0.65 -0.67 -0.65 -0.5 0.2 0.13 0.09 -0.06 0.09 -0.05 -0.16 -0.15 -0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 2.0 1.8 0.8 0.1 0.5 0 -0.4 0.3 0.6 1.6 3.1 2.1 1.0 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.8 7.1 4.8 7.8 7.4 8.2 5.9 0 0 0 0 0 3.0
Nucleus 0 -1.6 -2.1 -1.8 -1.9 -3.3 -3.0 -3.0 -3.3 0.4 -1.0 -2.4 -2.7 -2.4 -2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.6 0 -1.5 1.2 1.4 2.3 2.9 3.4 3.6 0 0 -1.5 0 -1.7 -1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.0 2.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2569 4.9411 4.8639 4.7328 4.5491 4.8823 4.2301 3.7587 3.3901 3.2643 2.9558 3.8183 3.6925 2.7044 2.808 2.9738 3.1281 2.9242
Actin 0.0054 0 0 0 0 0 0.0336 0 0.0088 0 0.0328 0.0008 0.0476 0 0.0024 0.0488 0.0437 0
Bud 0.0002 0 0 0 0 0 0.0004 0.0001 0.0002 0 0.0003 0.0001 0.0012 0 0.001 0.0007 0.0008 0.0001
Bud Neck 0.0007 0 0.0001 0 0.0003 0.0004 0.0012 0.0001 0.0003 0 0.0024 0.0013 0.0042 0 0.0003 0.001 0.0007 0.0005
Bud Periphery 0.0005 0 0 0 0 0 0.0014 0.0003 0.0004 0 0.0006 0.0004 0.0033 0 0.0023 0.0006 0.0032 0
Bud Site 0.0004 0 0 0 0 0 0.0015 0.0001 0.0003 0 0.0006 0.0002 0.0246 0 0.0031 0.0014 0.0006 0.0001
Cell Periphery 0.0004 0 0 0 0 0 0.0007 0.0001 0.0001 0 0.0002 0.0001 0.0011 0 0.0004 0.0001 0.0004 0
Cytoplasm 0.0004 0 0 0 0 0 0.0039 0.0001 0.0008 0 0.0032 0.0003 0.0202 0.0001 0.0013 0.0005 0.0007 0.0001
Cytoplasmic Foci 0.0006 0 0.0022 0 0 0 0.0072 0.0001 0.0036 0 0.0031 0.0001 0.023 0 0.0142 0.0138 0.0026 0.0003
Eisosomes 0.0001 0 0 0 0 0 0.0007 0 0.0001 0 0.0007 0 0.0005 0 0 0.0001 0.0008 0
Endoplasmic Reticulum 0.0005 0 0 0 0 0 0.0073 0 0.0017 0 0.0084 0.0009 0.0047 0 0.0002 0.0002 0.0033 0
Endosome 0.0014 0 0.0016 0 0 0 0.0096 0.0002 0.0069 0 0.0397 0.0019 0.0299 0 0.0029 0.0174 0.0052 0
Golgi 0.0009 0 0.0005 0 0 0 0.0039 0 0.0017 0 0.008 0.0002 0.0091 0 0.0014 0.0675 0.0035 0
Lipid Particles 0.0055 0 0.0047 0 0 0 0.0133 0.0007 0.0091 0 0.009 0.0002 0.0116 0 0.0009 0.004 0.0108 0
Mitochondria 0.0039 0 0.0001 0 0.0002 0 0.009 0.0046 0.0009 0 0.0049 0.0009 0.0072 0.0001 0.0067 0.0061 0.0632 0.0002
None 0.0003 0 0 0 0 0 0.008 0 0.0023 0 0.0115 0.0001 0.0024 0 0.0004 0.0001 0.0006 0.0001
Nuclear Periphery 0.0054 0.0003 0.0022 0.0002 0.0014 0.0001 0.0168 0.0015 0.007 0.0001 0.0521 0.0058 0.012 0.0004 0.0024 0.0002 0.0221 0.0003
Nucleolus 0.017 0.0104 0.011 0.0066 0.0888 0.0603 0.0247 0.0112 0.0125 0.0184 0.0672 0.0407 0.0093 0.0099 0.0122 0.0161 0.0578 0.0471
Nucleus 0.9489 0.9864 0.9756 0.993 0.9051 0.9383 0.8375 0.9774 0.9328 0.9812 0.7313 0.9432 0.7711 0.9893 0.9295 0.8167 0.7336 0.9495
Peroxisomes 0.001 0 0.0009 0 0 0 0.0071 0.0001 0.0058 0 0.002 0 0.0063 0 0.0016 0.0037 0.0384 0
Punctate Nuclear 0.0042 0.0028 0.0007 0.0002 0.0039 0.0007 0.0044 0.0006 0.0036 0.0001 0.0174 0.0009 0.0026 0.0001 0.0083 0.0002 0.0045 0.0017
Vacuole 0.0018 0 0.0001 0 0.0001 0 0.0048 0.0015 0.0006 0 0.0019 0.0013 0.0062 0 0.0059 0.0004 0.0014 0.0001
Vacuole Periphery 0.0005 0 0.0001 0 0 0 0.003 0.0011 0.0006 0 0.0028 0.0006 0.002 0 0.0028 0.0003 0.0021 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.9415 10.6924 11.2402 31.3423 18.0927 10.9582 25.6764 39.2201 23.1705 27.397
Translational Efficiency 1.4883 4.6675 3.968 0.9329 2.3505 3.4906 1.6901 1.0762 2.0945 1.7391

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
44 486 1410 184 1572 1365 304 1140 1616 1851 1714 1324

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1074.87 917.84 1139.18 1308.95 827.58 886.29 1346.33 1189.03 834.31 894.57 1175.92 1205.70
Standard Deviation 214.37 145.34 168.49 218.12 116.24 144.09 169.54 202.83 126.55 145.09 186.31 209.18
Intensity Change Log 2 — -0.227848 0.083834 0.284248 — 0.098880 0.702061 0.522814 — -0.076555 0.385683 0.392903

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003032 0.000952 0.000690 0.000872 0.000266 0.000591 0.000821 0.000955 0.000341 0.000686 0.000714 0.000944
Bud Neck 0.014098 0.014760 0.016560 0.013930 0.007379 0.010613 0.011161 0.019581 0.007562 0.011702 0.015603 0.018796
Bud Site 0.016031 0.001959 0.000930 0.001002 0.000965 0.002026 0.000644 0.001651 0.001375 0.002008 0.000880 0.001560
Cell Periphery 0.000434 0.000116 0.000036 0.000051 0.000074 0.000145 0.000025 0.000080 0.000084 0.000138 0.000034 0.000076
Cytoplasm 0.015073 0.003336 0.000095 0.000664 0.001985 0.002918 0.000051 0.003391 0.002341 0.003027 0.000087 0.003012
Cytoplasmic Foci 0.012224 0.001090 0.000012 0.000044 0.000285 0.000971 0.000015 0.000365 0.000610 0.001003 0.000012 0.000321
Eisosomes 0.000110 0.000035 0.000054 0.000034 0.000020 0.000031 0.000066 0.000032 0.000022 0.000032 0.000056 0.000033
Endoplasmic Reticulum 0.017968 0.006528 0.001479 0.001716 0.003010 0.005011 0.001811 0.002424 0.003418 0.005409 0.001538 0.002325
Endosome 0.014184 0.000977 0.000035 0.000064 0.000379 0.000967 0.000029 0.000455 0.000755 0.000969 0.000034 0.000401
Golgi 0.005938 0.000408 0.000041 0.000060 0.000134 0.001041 0.000046 0.000747 0.000292 0.000874 0.000042 0.000651
Lipid Particles 0.003653 0.000804 0.000032 0.000034 0.000262 0.000634 0.000026 0.000309 0.000354 0.000679 0.000031 0.000271
Mitochondria 0.005537 0.003228 0.001230 0.002100 0.001472 0.004040 0.001227 0.002127 0.001583 0.003827 0.001229 0.002124
Mitotic Spindle 0.003306 0.002937 0.000045 0.001548 0.000973 0.003844 0.000537 0.007574 0.001036 0.003606 0.000133 0.006736
None 0.002463 0.002458 0.000634 0.001330 0.002268 0.002617 0.000530 0.002964 0.002273 0.002575 0.000615 0.002737
Nuclear Periphery 0.004718 0.000521 0.000100 0.000246 0.000348 0.000477 0.000095 0.000671 0.000467 0.000488 0.000099 0.000612
Nuclear Periphery Foci 0.025840 0.001289 0.000010 0.000063 0.000491 0.000458 0.000014 0.000500 0.001182 0.000676 0.000011 0.000439
Nucleolus 0.057166 0.023994 0.017622 0.009216 0.020352 0.017048 0.013150 0.008049 0.021354 0.018872 0.016829 0.008211
Nucleus 0.715962* 0.929876* 0.959509* 0.965747* 0.958508* 0.942969* 0.968120* 0.946707* 0.951904* 0.939531* 0.961036* 0.949353*
Peroxisomes 0.009026 0.001042 0.000445 0.000545 0.000231 0.001022 0.001039 0.000312 0.000471 0.001027 0.000550 0.000345
Vacuole 0.063167 0.002750 0.000322 0.000619 0.000353 0.001716 0.000508 0.000911 0.002063 0.001987 0.000355 0.000870
Vacuole Periphery 0.010070 0.000939 0.000118 0.000115 0.000244 0.000863 0.000086 0.000195 0.000512 0.000883 0.000112 0.000184

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.07 3.41 2.93 -0.64 -2.24 -6.94 -9.93 -8.37 -5.28 -1.73 -6.72 -4.66 -8.41 -4.78 -3.12
Bud Neck -0.08 -0.49 -0.56 -0.76 -0.24 -3.06 -3.95 -11.03 -9.01 -7.67 -4.21 -8.58 -11.19 -8.32 -5.34
Bud Site 2.38 2.56 2.49 1.31 -1.71 -1.99 0.49 -3.29 -0.57 -6.03 -1.25 1.30 -1.75 -0.32 -5.63
Cell Periphery 1.51 1.92 1.82 2.81 -1.58 -2.12 4.69 -0.59 1.85 -5.00 -2.05 4.37 0.36 2.29 -4.51
Cytoplasm 1.85 2.39 2.30 2.12 -2.22 -1.03 4.22 -1.18 -0.19 -3.62 -0.81 4.76 -0.49 0.23 -3.72
Cytoplasmic Foci 1.90 2.09 2.08 2.29 -2.53 -2.13 2.92 0.18 2.08 -1.33 -1.41 3.23 1.59 2.60 -1.39
Eisosomes 2.89 2.14 2.83 -0.37 5.64 -6.05 -14.68 -8.01 -2.16 9.07 -5.37 -17.67 -6.95 -1.90 9.59
Endoplasmic Reticulum 2.84 4.28 4.18 7.42 -1.44 -5.07 3.40 -0.31 4.67 -3.36 -5.62 7.92 1.92 7.16 -6.22
Endosome 2.47 2.66 2.64 3.01 -2.55 -2.45 3.31 -0.05 2.36 -2.99 -0.92 3.94 1.92 3.06 -3.13
Golgi 1.82 1.95 1.93 2.02 -3.28 -1.67 1.51 -0.99 0.69 -1.17 -1.41 2.56 -0.54 0.69 -1.22
Lipid Particles 2.17 2.84 2.82 2.49 -1.10 -1.37 1.79 0.04 1.30 -1.34 -1.38 2.50 0.64 1.86 -1.41
Mitochondria 0.85 1.83 1.27 0.48 -3.13 -2.29 0.11 -1.96 1.45 -5.74 -2.33 0.38 -1.82 1.51 -5.95
Mitotic Spindle -0.17 1.67 -0.46 -0.38 -1.12 -2.19 -0.28 -4.43 -3.25 -3.81 -2.39 1.65 -4.46 -3.25 -4.91
None -0.15 2.29 1.09 2.09 -2.72 -0.67 5.31 -1.19 -0.51 -5.60 -0.71 5.24 -0.90 -0.26 -5.71
Nuclear Periphery 1.31 1.46 1.37 1.69 -4.19 -1.47 1.89 -4.95 -3.82 -6.03 -0.32 3.38 -3.10 -3.52 -7.67
Nuclear Periphery Foci 2.53 2.66 2.65 2.00 -2.25 0.49 2.04 0.16 -0.43 -2.78 1.42 3.26 1.85 0.64 -2.97
Nucleolus 3.01 3.61 3.92 2.81 1.57 2.05 3.60 3.98 2.33 0.44 1.58 2.60 4.71 3.55 2.66
Nucleus -4.42 -5.07 -4.87 -2.31 0.99 3.41 -1.66 6.42 2.87 7.54 2.87 -2.42 4.48 1.81 7.15
Peroxisomes 2.02 2.17 2.12 1.11 -1.35 -2.38 -6.96 -1.97 2.05 5.87 -2.01 -0.73 0.62 2.42 2.34
Vacuole 3.36 3.52 3.47 1.76 -2.43 -3.37 -2.63 -3.29 -0.91 -2.32 0.08 2.95 -0.05 -0.15 -3.32
Vacuole Periphery 2.36 2.58 2.57 1.90 -1.12 -2.40 2.24 0.54 2.61 -3.47 -1.47 3.09 2.40 3.18 -2.92
Standard name
Human Ortholog
Description Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rox1

Rox1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rox1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available