Standard name
Human Ortholog
Description Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0.06 0 0.14 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0.09 0 0.11 0.07 0 0.05 0 0 0 0.05 0 0 0.06 0.12 0 0 0 0 0 0
Golgi 0 0.06 0.32 0.2 0.25 0 0 0 0 0 0 0 0.15 0.22 0.37 0.35 0.34 0.11 0 0.07 0 0.07 0.07 0.09
Mitochondria 0.88 0.9 0.75 0.87 0.6 0.88 0.73 0.79 0.88 0.86 0.91 0.92 0.93 0.85 0.74 0.06 0.12 0.1 0.79 0.77 0.8 0.72 0.73 0.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0.08 0 0.05 0 0.08 0.1 0.07 0.09 0.1 0.07 0.1 0.07 0.05 0 0 0.08 0.12 0.08 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0.06 0.05 0.05 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0 0.06 0.09 0 0 0 0 0 0 0 0 0.06 0.08 0.05 0.07 0.08 0.18 0.08 0 0.09 0.1 0.09 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0.06 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.13 0.1 0 0.15 0.06 0.11 0.07 0 0.07 0 0 0.07 0.08 0.11 0.16 0.27 0.24 0 0.06 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 5 1 2 0 0 0 1 0 1 0 0 4 7 6 3 3 1 6 7 2 5 18 24
Bud 2 3 1 1 1 5 4 2 6 9 2 5 2 2 2 6 3 6 3 1 0 1 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1
Bud Site 0 0 0 0 0 1 1 2 1 0 0 0 0 0 1 0 0 5 – – – – – –
Cell Periphery 15 24 10 4 4 0 4 2 6 5 2 1 17 36 27 22 10 8 0 2 0 0 0 2
Cytoplasm 1 2 2 6 9 9 14 17 11 12 2 5 5 8 12 6 3 4 0 1 0 0 0 2
Endoplasmic Reticulum 0 12 1 0 1 4 5 1 3 0 4 2 6 1 14 6 3 2 0 1 0 0 1 5
Endosome 16 6 11 7 13 3 35 25 5 17 5 2 17 31 23 6 11 14 18 16 4 12 22 21
Golgi 6 27 55 38 35 0 11 11 2 3 1 6 69 129 266 56 61 13 19 32 8 22 43 57
Mitochondria 423 401 130 164 85 243 229 295 302 281 179 191 422 507 535 10 21 12 403 364 155 229 471 452
Nucleus 2 3 0 0 1 2 6 3 5 3 0 2 2 0 2 4 3 6 0 1 0 0 0 0
Nuclear Periphery 39 15 8 2 12 27 23 32 33 23 20 14 25 13 29 13 21 10 4 1 0 2 2 7
Nucleolus 26 8 4 4 2 17 15 20 18 10 12 8 9 4 8 3 4 5 2 1 0 0 2 3
Peroxisomes 27 9 10 16 6 1 10 3 6 4 1 1 26 46 37 11 15 22 40 9 18 31 56 46
SpindlePole 9 3 2 1 6 5 11 6 3 7 1 7 6 7 4 18 10 7 3 1 1 0 3 2
Vac/Vac Membrane 66 59 18 3 21 16 35 25 14 24 7 9 31 46 78 26 47 29 2 26 0 7 10 11
Unique Cell Count 481 446 174 188 142 275 315 373 343 325 197 207 456 597 726 162 177 121 509 472 195 319 642 644
Labelled Cell Count 632 577 253 248 196 333 403 445 415 399 236 253 641 837 1044 190 215 144 509 472 195 319 642 644


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 7.4 8.5 8.2 8.7 5.6 7.8 7.6 6.8 6.9 6.8 6.6 8.6 9.3 9.5 9.0 9.5 8.5 7.5 7.7 8.2
Std Deviation (1e-4) 1.3 1.3 1.4 1.7 1.6 1.5 1.8 1.7 1.6 1.7 1.6 1.6 1.4 1.7 1.6 1.9 2.3 2.1 1.7 1.7 1.9
Intensity Change (Log2) – – – -0.06 0.02 -0.6 -0.13 -0.17 -0.33 -0.3 -0.32 -0.37 0.01 0.13 0.15 0.08 0.15 -0.01 -0.18 -0.14 -0.05

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.8 -1.3 0 0 0 -2.5 -2.6 -2.6 -3.1 -1.1 0.1 -1.2 2.4 0 0.3
Cytoplasm 0 0 0 2.0 2.0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.1 0.9 -3.1 1.7 0.2 -3.0 -0.5 -1.8 -2.9 -1.4 -0.6 -2.0 -1.1 0 1.6
Golgi -2.5 -1.4 -10.0 -8.7 -9.6 -10.6 -10.2 -8.4 -7.6 -4.7 -2.7 1.2 0.6 0.6 -4.2
Mitochondria 3.0 -2.8 3.8 -0.5 1.1 3.9 3.3 4.2 4.7 6.1 3.1 -0.3 -12.7 -11.9 -11.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 1.4 2.0 1.2 1.7 2.0 1.1 2.0 0.9 0.4 0 -0.4 1.3 2.5 1.3
Nucleolus 0 0 1.9 1.3 1.6 1.6 0 1.8 0.9 0 0 0 0 0 0
Peroxisomes 1.0 -0.6 0 -1.4 0 -2.5 0 -3.0 -3.1 0 0.9 -0.3 0.4 1.0 3.4
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 3.9 2.3 0
Vacuole -3.6 1.2 -1.8 0.3 -1.5 -2.8 -1.1 -2.6 -2.3 -1.5 -1.1 0.2 1.5 3.9 3.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.849 3.2159 2.7825 2.1886 2.0715 2.8678 4.4163 4.8501 4.5476 4.7647 3.7768 4.6626 6.3197 7.1638 6.3874 6.1934 5.9878 6.3359
Actin 0.0023 0.0013 0.0015 0.0006 0.0003 0.0005 0.0076 0.0021 0.0008 0.0005 0.0004 0.0011 0.0091 0.0027 0.0118 0.0006 0.0052 0.0036
Bud 0.0001 0.0002 0.0003 0 0.0001 0.0002 0.0001 0.0012 0.0005 0.0004 0.0001 0.0001 0.0002 0.0004 0.0024 0 0.0001 0.0001
Bud Neck 0.0001 0.0001 0.0002 0 0 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0003 0 0.0001 0.0002
Bud Periphery 0.0004 0.0004 0.0016 0.0001 0.0002 0.0004 0.0003 0.0023 0.0006 0.0017 0.0003 0.0002 0.0003 0.0008 0.0027 0.0001 0.0002 0.0003
Bud Site 0.0003 0.0009 0.0014 0 0.0001 0.0007 0.0008 0.0068 0.005 0.0003 0.0001 0.0001 0.0005 0.0006 0.0022 0 0.0001 0.0002
Cell Periphery 0.0002 0.0002 0.0023 0 0.0001 0.0001 0.0002 0.0005 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0035 0 0.0001 0.0001
Cytoplasm 0.0001 0.0002 0.004 0 0.0001 0.0003 0.0003 0.0003 0.0001 0.0003 0.0001 0.0001 0.0006 0.0003 0.0038 0 0.0003 0.0005
Cytoplasmic Foci 0.0026 0.0061 0.0053 0.001 0.0025 0.002 0.0057 0.0076 0.0056 0.0017 0.0047 0.0045 0.0084 0.0113 0.0078 0.0003 0.0064 0.0043
Eisosomes 0.0002 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0007 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0003 0.0002 0.0002 0 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003 0 0.0001 0.0007 0.0002 0.0005 0 0 0.0016
Endosome 0.0155 0.0182 0.0207 0.0035 0.0317 0.0081 0.0196 0.0265 0.0117 0.0117 0.0141 0.0096 0.0305 0.0335 0.0179 0.0022 0.0073 0.0187
Golgi 0.0143 0.0221 0.0183 0.008 0.011 0.0112 0.0215 0.0428 0.0196 0.01 0.0502 0.0283 0.0328 0.0682 0.0214 0.0042 0.0195 0.0269
Lipid Particles 0.0114 0.0084 0.0045 0.0093 0.0188 0.0098 0.0149 0.0085 0.0106 0.0013 0.0009 0.0037 0.0229 0.0066 0.0051 0.0005 0.0044 0.016
Mitochondria 0.9393 0.9238 0.9241 0.9727 0.8859 0.9583 0.9023 0.8693 0.9214 0.9648 0.8841 0.9419 0.8444 0.8042 0.8798 0.9904 0.9069 0.8883
None 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0.0006 0.0001 0.001 0 0.0015 0.0007
Nuclear Periphery 0.0002 0.0002 0 0 0.0001 0.0001 0.0003 0.0003 0 0.0001 0 0.0003 0.0011 0.0016 0.0009 0 0.0018 0.0022
Nucleolus 0.0001 0.0005 0.0001 0 0.0001 0.0001 0.001 0.0002 0.0001 0.0001 0 0 0.0001 0.0001 0.0002 0 0.0001 0.0002
Nucleus 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0.0003 0.0003 0.0012 0 0.0002 0.0004
Peroxisomes 0.0088 0.0085 0.0126 0.0036 0.0391 0.0053 0.0171 0.017 0.0163 0.0019 0.0373 0.0072 0.0413 0.0537 0.0251 0.0008 0.0269 0.0251
Punctate Nuclear 0 0 0 0 0 0 0.0002 0 0.0001 0 0 0 0.0017 0 0.0023 0 0.0178 0.0054
Vacuole 0.0014 0.0033 0.0012 0.0002 0.004 0.0011 0.0027 0.0062 0.0029 0.0016 0.0017 0.0006 0.0014 0.0055 0.0032 0.0001 0.0003 0.0012
Vacuole Periphery 0.0023 0.0049 0.0013 0.0006 0.0057 0.0013 0.0045 0.0073 0.0042 0.0027 0.0058 0.0021 0.0025 0.0094 0.0061 0.0005 0.0007 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.8646 12.3739 14.4288 23.7169 20.5106 13.8343 22.3415 19.9043 25.8173 26.5979
Translational Efficiency 0.8429 1.1998 0.7964 0.5701 0.5145 1.0043 0.8281 0.8094 0.8261 0.639

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1949 472 1367 110 908 1727 430 888 2857 2199 1797 998

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 814.87 913.01 1069.22 1124.68 823.64 926.56 1023.49 1051.75 817.66 923.65 1058.28 1059.79
Standard Deviation 109.27 143.57 157.55 183.66 120.75 140.60 130.23 179.18 113.12 141.35 152.71 181.13
Intensity Change Log 2 — 0.164061 0.391917 0.464873 — 0.169871 0.313411 0.352706 — 0.166984 0.352988 0.409579

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000518 0.011044 0.002161 0.021329 0.000805 0.001009 0.008227 0.007366 0.000609 0.003163 0.003613 0.008905
Bud Neck 0.000791 0.002317 0.001952 0.004482 0.002523 0.000711 0.004544 0.001869 0.001341 0.001055 0.002572 0.002157
Bud Site 0.001441 0.014079 0.004399 0.022495 0.002545 0.001512 0.018000 0.016593 0.001792 0.004209 0.007653 0.017244
Cell Periphery 0.000192 0.000476 0.000163 0.001309 0.000469 0.000134 0.000357 0.001695 0.000280 0.000207 0.000209 0.001653
Cytoplasm 0.000339 0.001898 0.000894 0.001789 0.000516 0.000595 0.001619 0.002007 0.000395 0.000875 0.001068 0.001983
Cytoplasmic Foci 0.009850 0.021974 0.050970 0.042322 0.021217 0.017111 0.048741 0.021089 0.013462 0.018155 0.050437 0.023429
Eisosomes 0.000030 0.000127 0.000056 0.000246 0.000044 0.000045 0.000093 0.000104 0.000034 0.000062 0.000065 0.000120
Endoplasmic Reticulum 0.000174 0.000254 0.000539 0.001157 0.000255 0.000127 0.000445 0.001157 0.000200 0.000154 0.000516 0.001157
Endosome 0.028550 0.057740 0.045759 0.067150 0.030682 0.034457 0.059136 0.040151 0.029227 0.039454 0.048960 0.043126
Golgi 0.017099 0.044616 0.074041 0.098311 0.026117 0.028280 0.049468 0.069995 0.019965 0.031786 0.068161 0.073116
Lipid Particles 0.004014 0.002853 0.003925 0.000578 0.009952 0.003780 0.002720 0.000581 0.005902 0.003581 0.003637 0.000580
Mitochondria 0.902825 0.774025 0.720734 0.664242 0.856277 0.877849 0.697858 0.773013 0.888031 0.855564 0.715260 0.761024
Mitotic Spindle 0.001745 0.002577 0.022503 0.017333 0.004715 0.004476 0.022762 0.009639 0.002689 0.004068 0.022565 0.010487
None 0.000161 0.000228 0.000385 0.000286 0.000187 0.000181 0.000258 0.000337 0.000169 0.000191 0.000355 0.000332
Nuclear Periphery 0.000091 0.000058 0.001798 0.000223 0.000201 0.000093 0.000203 0.000158 0.000126 0.000086 0.001416 0.000165
Nuclear Periphery Foci 0.000255 0.000105 0.001693 0.000350 0.001028 0.000264 0.000527 0.000177 0.000501 0.000230 0.001414 0.000196
Nucleolus 0.000167 0.000050 0.000327 0.000084 0.000439 0.000229 0.000262 0.000041 0.000253 0.000190 0.000312 0.000045
Nucleus 0.000109 0.000078 0.000208 0.000090 0.000263 0.000239 0.000231 0.000068 0.000158 0.000204 0.000214 0.000070
Peroxisomes 0.024247 0.054582 0.013147 0.028214 0.035380 0.020601 0.065596 0.017392 0.027785 0.027895 0.025698 0.018584
Vacuole 0.002645 0.005647 0.023432 0.021293 0.002922 0.004243 0.010129 0.017225 0.002733 0.004544 0.020249 0.017673
Vacuole Periphery 0.004757 0.005271 0.030915 0.006718 0.003464 0.004066 0.008821 0.019345 0.004346 0.004325 0.025628 0.017953

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.08 -5.01 -2.74 -1.28 -2.52 -0.98 -3.67 -4.36 -4.21 0.34 -4.41 -5.49 -5.25 -3.42 -3.16
Bud Neck -3.63 -3.48 -2.70 -1.54 -1.81 1.81 -1.74 0.62 -3.44 3.86 0.81 -2.88 -1.79 -3.23 1.01
Bud Site -3.82 -3.96 -2.86 -1.05 -2.44 1.13 -4.38 -5.36 -6.04 0.31 -2.93 -5.66 -6.52 -5.29 -3.76
Cell Periphery -4.21 1.20 -2.06 -1.53 -2.12 1.38 0.45 -2.23 -3.16 -2.69 0.89 0.88 -3.05 -3.25 -3.25
Cytoplasm -2.11 -4.81 -2.31 0.11 -1.35 -0.27 -4.21 -3.90 -3.73 -1.08 -2.07 -5.88 -5.18 -3.00 -2.85
Cytoplasmic Foci -4.28 -14.13 -3.55 -2.14 0.91 1.73 -5.31 0.05 -1.67 5.34 -3.22 -14.46 -4.45 -2.23 8.53
Eisosomes -7.99 -9.36 -3.03 -1.64 -2.67 -0.27 -6.39 -5.71 -5.65 -0.87 -7.79 -10.68 -7.05 -4.58 -4.48
Endoplasmic Reticulum -1.62 -6.61 -2.61 -2.39 -1.63 2.47 -2.53 -3.19 -3.73 -2.55 1.54 -6.91 -3.81 -4.02 -2.56
Endosome -4.85 -5.65 -3.27 -0.73 -1.79 -1.06 -5.06 -2.34 -1.59 3.36 -3.88 -7.39 -4.22 -1.04 1.64
Golgi -5.61 -14.37 -4.57 -2.92 -1.34 -0.69 -4.54 -7.44 -7.40 -2.95 -5.58 -14.69 -10.08 -7.61 -0.83
Lipid Particles 1.34 0.11 4.95 3.87 5.42 3.39 4.01 5.43 4.90 3.78 2.66 2.67 7.36 5.68 6.36
Mitochondria 8.28 17.75 6.58 2.83 1.52 -2.07 8.29 5.89 8.48 -3.74 4.51 19.01 11.08 7.92 -3.48
Mitotic Spindle -1.90 -9.46 -3.40 -3.22 1.05 0.19 -4.82 -2.47 -3.00 3.41 -1.82 -10.41 -5.10 -4.05 5.14
None -2.82 -10.59 -3.83 -1.76 3.19 0.17 -2.82 -4.84 -3.68 -2.70 -0.73 -11.04 -6.37 -3.99 0.93
Nuclear Periphery 1.33 -4.10 -1.52 -1.94 3.71 1.13 -0.02 0.43 -1.67 1.03 1.10 -4.05 -0.85 -2.28 3.93
Nuclear Periphery Foci 1.54 -4.09 -0.47 -1.40 3.51 1.75 1.14 1.95 1.23 2.10 1.73 -2.89 1.96 0.55 4.65
Nucleolus 1.88 -1.40 1.19 -0.93 2.52 1.00 1.03 2.48 1.28 2.72 0.53 -0.52 3.12 1.25 3.75
Nucleus 0.85 -1.85 0.47 -0.37 2.43 0.20 0.43 2.49 1.22 2.66 -0.37 -1.09 2.31 1.21 3.74
Peroxisomes -4.09 4.12 -0.41 2.27 -1.60 3.55 -3.66 4.01 0.98 6.13 -0.02 0.70 2.91 2.77 2.16
Vacuole -2.58 -12.37 -2.03 -1.69 0.21 -1.79 -4.98 -6.50 -5.96 -2.83 -3.54 -13.13 -6.91 -5.99 1.02
Vacuole Periphery -0.48 -11.99 -1.10 -0.75 8.78 -1.01 -3.61 -7.70 -7.41 -4.26 0.04 -12.48 -7.36 -7.36 3.12
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Isa2

Isa2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Isa2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available