Standard name
Human Ortholog
Description Component of the CCR4-NOT core complex, involved in mRNA decapping; involved intranscription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not5p and Not3p is mutated in cancers
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.1 0 0 0.05 0.05 0 0.05 0.08 0 0.05 0 0 0 0.14 0.1 0.09 0 0 0 0 0 0
Cytoplasm 0.96 0.95 0.92 0.94 0.93 0.87 0.73 0.68 0.73 0.64 0.59 0.6 0.98 0.97 0.99 0.87 0.9 0.89 0.9 0.95 0.86 0.94 0.89 0.92
Endoplasmic Reticulum 0 0.07 0 0 0.07 0.08 0.21 0.22 0.15 0.19 0.14 0.15 0 0 0 0 0 0 0.05 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0 0.12 0.12 0.3 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0.05 0 0.06 0 0 0 0.06 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 4 1 5 9 24 11 17 16 24 0 0 0 1 1 2 0 0 0 0 3 0
Bud 0 0 0 0 0 0 2 2 1 2 2 1 0 0 0 0 1 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 9 14 15 11 18 29 25 16 35 10 22 14 15 17 36 27 32 0 0 0 0 0 0
Cytoplasm 207 208 123 324 316 309 395 442 221 300 206 240 373 505 701 220 235 306 187 207 117 149 256 310
Endoplasmic Reticulum 3 15 5 15 24 27 114 141 46 87 48 61 9 18 12 11 6 9 11 7 8 2 8 10
Endosome 1 0 0 0 0 0 1 1 0 1 1 0 0 0 2 0 0 0 1 0 0 0 4 0
Golgi 0 0 1 0 0 0 11 8 2 5 4 11 0 0 0 4 1 4 1 0 1 0 1 1
Mitochondria 0 0 0 0 0 15 30 18 35 54 105 63 0 0 1 4 4 3 1 0 1 0 2 2
Nucleus 1 0 1 1 1 2 2 4 1 3 1 9 4 4 3 3 1 5 0 0 0 2 1 4
Nuclear Periphery 0 0 0 0 0 0 0 3 1 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 0 1 2 6 14 29 14 22 10 25 0 0 1 16 4 16 0 0 2 3 2 2
Unique Cell Count 216 219 134 344 340 355 542 648 303 466 350 403 379 520 711 253 261 345 209 217 136 159 287 338
Labelled Cell Count 218 234 145 360 355 382 607 698 348 527 403 456 400 542 737 295 280 379 209 217 136 159 287 338


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.2 16.4 14.9 14.2 14.1 12.3 11.6 12.0 10.5 10.7 9.8 9.7 14.8 14.6 14.2 17.1 18.2 16.6 14.4 14.1 14.6
Std Deviation (1e-4) 2.0 2.3 1.7 1.9 1.8 2.0 1.7 1.7 1.8 1.7 1.9 2.2 2.1 2.1 1.8 3.3 2.9 3.2 1.7 2.3 2.4
Intensity Change (Log2) – – – -0.07 -0.08 -0.28 -0.36 -0.31 -0.5 -0.47 -0.6 -0.62 -0.01 -0.03 -0.07 0.2 0.29 0.15 -0.05 -0.08 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 2.5 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.5 -3.2 -2.1 -2.2 -3.2 -2.0 -1.1 -3.4 -2.0 -3.0 -3.8 0 1.1 0 -0.4
Cytoplasm 1.0 0.4 -1.5 -4.6 -5.5 -4.4 -6.1 -6.9 -6.9 3.7 2.8 4.6 -1.4 -0.6 -1.0
Endoplasmic Reticulum 0.3 1.4 1.5 4.7 4.9 3.4 4.2 3.1 3.5 0 0 0 0.3 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.8 0 4.1 4.1 7.2 4.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 3.0 0 0 0 3.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.8711 11.4996 10.8304 9.8146 9.9177 10.7731 7.0825 9.9217 9.4165 7.202 7.62 9.5112 6.8354 8.3737 8.1019 7.3369 7.3125 8.5275
Actin 0.0009 0.0004 0.0009 0.001 0.0003 0.0005 0.0165 0.0017 0.0089 0.0013 0.0403 0.0035 0.015 0.0027 0.0112 0.005 0.0273 0.0023
Bud 0.0004 0.0004 0.0005 0.0011 0.0003 0.0004 0.0007 0.0005 0.0004 0.0043 0.0011 0.0026 0.0017 0.0037 0.0025 0.004 0.0003 0.0002
Bud Neck 0.0004 0.0003 0.0005 0.0009 0.0006 0.0012 0.0032 0.0002 0.0008 0.001 0.0022 0.0009 0.0004 0.0002 0.0019 0.0005 0.0007 0.0013
Bud Periphery 0.0004 0.0002 0.0003 0.0007 0.0002 0.0004 0.0008 0.0002 0.0004 0.0035 0.0011 0.0115 0.0007 0.0005 0.001 0.0055 0.0002 0.0001
Bud Site 0.0006 0.0005 0.0003 0.0007 0.0001 0.0001 0.003 0.0068 0.0013 0.0034 0.0074 0.0028 0.0021 0.0065 0.0142 0.0106 0.0007 0.0001
Cell Periphery 0.0008 0.0002 0.0003 0.0003 0.0001 0.0003 0.0006 0.0004 0.0003 0.0004 0.0002 0.0016 0.0002 0.0004 0.0007 0.0005 0.0001 0.0001
Cytoplasm 0.7977 0.9258 0.8764 0.8395 0.8718 0.8402 0.7022 0.9304 0.7952 0.6742 0.6229 0.8232 0.718 0.9371 0.8315 0.7642 0.7473 0.8352
Cytoplasmic Foci 0.0109 0.0111 0.0115 0.0167 0.014 0.0148 0.0278 0.0105 0.0216 0.0232 0.0406 0.0147 0.0453 0.009 0.0209 0.0194 0.0367 0.0209
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0006 0 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0983 0.019 0.0321 0.0268 0.0265 0.0682 0.0734 0.0203 0.0316 0.0153 0.0108 0.0334 0.0447 0.01 0.0149 0.0161 0.0075 0.0738
Endosome 0.0532 0.0251 0.0468 0.0661 0.0615 0.0463 0.0642 0.0139 0.0691 0.1087 0.1826 0.0581 0.0756 0.0139 0.0498 0.0756 0.0855 0.052
Golgi 0.0061 0.0038 0.0046 0.0034 0.0066 0.004 0.0116 0.0014 0.0149 0.0099 0.0373 0.0085 0.0087 0.0026 0.0141 0.0043 0.0184 0.0034
Lipid Particles 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0085 0.0004 0.0161 0.0213 0.0102 0.0031 0.0164 0.0005 0.0089 0.0017 0.0215 0.0001
Mitochondria 0.0039 0.0035 0.0057 0.0034 0.0049 0.0095 0.007 0.0004 0.0134 0.0682 0.0074 0.0189 0.0032 0.0036 0.0036 0.0569 0.0147 0.0012
None 0.0017 0.0008 0.0008 0.0008 0.0007 0.0013 0.0131 0.0038 0.0022 0.0015 0.0012 0.0014 0.0101 0.002 0.0018 0.0013 0.0055 0.0008
Nuclear Periphery 0.0055 0.0012 0.0024 0.0034 0.0011 0.0018 0.0185 0.0011 0.0048 0.0033 0.0028 0.0025 0.0241 0.0012 0.0018 0.004 0.0025 0.0022
Nucleolus 0 0 0 0 0 0 0.0011 0.0001 0.0003 0.0011 0.0005 0.0002 0.0004 0.0002 0.0003 0.0006 0.0001 0
Nucleus 0.002 0.0011 0.0013 0.0024 0.0009 0.0013 0.0122 0.001 0.0015 0.0072 0.0015 0.0014 0.0045 0.0011 0.0012 0.002 0.0011 0.0013
Peroxisomes 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0118 0.0001 0.0061 0.0063 0.0082 0.0006 0.0071 0.0005 0.0023 0.0041 0.0175 0.0001
Punctate Nuclear 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0106 0.0003 0.0008 0.001 0.0041 0.0002 0.0094 0.0002 0.0005 0.0004 0.0027 0.0002
Vacuole 0.0134 0.0048 0.0115 0.0259 0.0075 0.0069 0.0103 0.0058 0.0069 0.0359 0.0127 0.0081 0.0091 0.0035 0.0151 0.0183 0.0064 0.0034
Vacuole Periphery 0.0033 0.0014 0.0037 0.0063 0.0026 0.0027 0.0026 0.0007 0.0026 0.0088 0.0048 0.003 0.0032 0.0006 0.0019 0.0048 0.0029 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.8104 42.784 32.6174 49.3889 46.327 38.0048 47.9995 46.5949 43.3206 53.6432
Translational Efficiency 1.5382 1.8087 1.8743 1.1847 1.4009 2.1784 1.3429 1.2201 1.506 1.5052

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1658 888 289 1397 1637 1961 112 3 3295 2849 401 1400

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1019.65 1058.63 1834.51 1160.86 1145.46 1220.54 1578.51 2071.48 1082.15 1170.07 1763.01 1162.81
Standard Deviation 149.75 265.94 213.40 203.09 170.87 179.58 157.33 212.82 172.47 223.30 230.05 207.43
Intensity Change Log 2 — 0.054124 0.847321 0.187120 — 0.091592 0.462636 0.854735 — 0.074068 0.656609 0.578139

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000225 0.000315 0.001095 0.000428 0.000255 0.000192 0.000745 0.000085 0.000240 0.000230 0.000997 0.000427
Bud Neck 0.000430 0.001308 0.008235 0.002400 0.000282 0.000319 0.002424 0.001079 0.000356 0.000627 0.006612 0.002397
Bud Site 0.017010 0.026478 0.031294 0.026759 0.020213 0.014970 0.031604 0.022899 0.018601 0.018557 0.031380 0.026751
Cell Periphery 0.000241 0.000499 0.002378 0.001587 0.000356 0.000209 0.000658 0.000651 0.000298 0.000300 0.001898 0.001585
Cytoplasm 0.652732* 0.669843* 0.481644* 0.639851* 0.648159* 0.688821* 0.347873* 0.899419* 0.650460* 0.682906* 0.444282* 0.640407*
Cytoplasmic Foci 0.217272* 0.145928 0.074839 0.080060 0.224257* 0.179368 0.162235 0.001394 0.220742* 0.168945 0.099249 0.079891
Eisosomes 0.000009 0.000010 0.000009 0.000134 0.000007 0.000006 0.000012 0.000006 0.000008 0.000007 0.000010 0.000134
Endoplasmic Reticulum 0.013297 0.012694 0.006385 0.014758 0.018268 0.011024 0.027026 0.000017 0.015767 0.011544 0.012150 0.014727
Endosome 0.009599 0.021426 0.030910 0.015435 0.009266 0.009546 0.051058 0.000656 0.009434 0.013249 0.036537 0.015403
Golgi 0.027381 0.020907 0.049109 0.016012 0.028451 0.025892 0.021397 0.000363 0.027913 0.024338 0.041369 0.015978
Lipid Particles 0.001851 0.003470 0.020177 0.007823 0.001371 0.001241 0.008296 0.000184 0.001613 0.001936 0.016858 0.007807
Mitochondria 0.003159 0.000557 0.000048 0.000354 0.001086 0.000974 0.000501 0.000530 0.002129 0.000844 0.000175 0.000354
Mitotic Spindle 0.000938 0.001351 0.015552 0.000718 0.000914 0.001806 0.007841 0.000006 0.000926 0.001664 0.013398 0.000717
None 0.001737 0.001434 0.000181 0.001058 0.000337 0.000292 0.000028 0.000089 0.001042 0.000648 0.000139 0.001056
Nuclear Periphery 0.000093 0.001304 0.000631 0.000275 0.000098 0.000083 0.000408 0.000002 0.000096 0.000463 0.000569 0.000274
Nuclear Periphery Foci 0.000983 0.003982 0.025026 0.002321 0.001376 0.000678 0.009457 0.000007 0.001178 0.001708 0.020678 0.002316
Nucleolus 0.000341 0.000736 0.000065 0.000256 0.000026 0.000065 0.000052 0.000206 0.000184 0.000274 0.000061 0.000256
Nucleus 0.002596 0.009148 0.005042 0.026503 0.001166 0.001539 0.003815 0.001147 0.001886 0.003911 0.004699 0.026449
Peroxisomes 0.000266 0.000485 0.000303 0.000661 0.000208 0.000392 0.000536 0.000323 0.000237 0.000421 0.000368 0.000660
Vacuole 0.020114 0.046835 0.220765* 0.143814 0.016179 0.034672 0.295567* 0.067993 0.018159 0.038463 0.241658* 0.143652
Vacuole Periphery 0.029726 0.031291 0.026312 0.018794 0.027723 0.027911 0.028468 0.002946 0.028731 0.028964 0.026914 0.018760

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.83 -5.57 -2.94 -1.81 4.27 NaN NaN NaN NaN NaN 0.29 -6.33 -3.11 -3.43 4.55
Bud Neck -3.25 -9.71 -8.89 -3.10 7.43 NaN NaN NaN NaN NaN -3.11 -10.31 -9.30 -7.51 7.08
Bud Site -4.68 -5.53 -5.13 -0.08 1.74 NaN NaN NaN NaN NaN 0.03 -5.68 -4.42 -4.35 1.88
Cell Periphery -1.90 -8.46 -11.83 -6.19 2.75 NaN NaN NaN NaN NaN -0.03 -8.35 -11.38 -10.69 1.29
Cytoplasm -1.38 9.07 1.21 2.28 -8.13 NaN NaN NaN NaN NaN -4.35 12.92 1.06 4.21 -11.31
Cytoplasmic Foci 9.35 20.49 23.46 10.17 -0.77 NaN NaN NaN NaN NaN 10.31 18.34 30.23 19.38 3.19
Eisosomes -1.22 -0.66 -4.36 -4.29 -4.33 NaN NaN NaN NaN NaN 0.67 -2.28 -4.39 -4.40 -4.30
Endoplasmic Reticulum 0.32 3.82 -0.74 -0.97 -4.24 NaN NaN NaN NaN NaN 3.16 1.87 0.60 -1.86 -1.24
Endosome -6.89 -9.70 -6.15 3.34 6.83 NaN NaN NaN NaN NaN -5.56 -12.99 -7.02 -2.17 9.61
Golgi 3.81 -4.36 8.27 3.18 7.11 NaN NaN NaN NaN NaN 3.36 -3.51 11.11 7.29 7.19
Lipid Particles -2.24 -9.86 -8.39 -5.39 6.78 NaN NaN NaN NaN NaN -1.11 -10.88 -10.24 -9.88 6.42
Mitochondria 3.49 4.20 3.78 1.87 -4.75 NaN NaN NaN NaN NaN 3.15 4.78 4.51 3.50 -1.26
Mitotic Spindle -1.19 -6.12 0.56 2.48 6.23 NaN NaN NaN NaN NaN -1.96 -6.72 0.71 2.48 6.82
None 0.46 2.64 1.02 0.90 -2.83 NaN NaN NaN NaN NaN 1.25 3.02 -0.03 -1.26 -2.98
Nuclear Periphery -2.33 -5.30 -2.79 1.97 3.24 NaN NaN NaN NaN NaN -2.23 -5.95 -2.79 1.09 3.19
Nuclear Periphery Foci -6.29 -11.03 -4.22 3.31 10.38 NaN NaN NaN NaN NaN -1.79 -11.68 -3.15 -2.07 11.01
Nucleolus -1.45 1.43 0.45 2.40 -5.28 NaN NaN NaN NaN NaN -0.85 1.26 -0.70 0.37 -5.46
Nucleus -4.22 -4.27 -9.27 -5.76 -7.77 NaN NaN NaN NaN NaN -4.05 -3.87 -9.58 -8.63 -7.82
Peroxisomes -2.52 -1.37 -2.81 -0.97 -2.19 NaN NaN NaN NaN NaN -1.53 -3.48 -3.02 -0.68 -1.81
Vacuole -7.93 -14.03 -19.48 -13.97 5.16 NaN NaN NaN NaN NaN -11.04 -16.94 -20.05 -16.41 6.87
Vacuole Periphery -0.49 1.72 5.48 4.31 1.84 NaN NaN NaN NaN NaN -0.12 1.25 6.09 5.75 2.35
Standard name
Human Ortholog
Description Component of the CCR4-NOT core complex, involved in mRNA decapping; involved intranscription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not5p and Not3p is mutated in cancers
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Not5

Not5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Not5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available