Standard name | |
---|---|
Human Ortholog | |
Description | Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 1 | 0 | 1 | 1 | 4 | 1 | 1 | 3 | 0 | 1 | 2 | 5 | 5 | 2 | 1 | 1 | 3 | 3 | 3 | 9 | 8 | 11 |
Bud | 1 | 4 | 1 | 0 | 0 | 2 | 6 | 10 | 13 | 16 | 19 | 12 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 9 | 1 | 2 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 9 | 3 | 6 | 10 | 18 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 3 | 0 | 4 | 6 | 7 | 9 | 10 | 8 | 5 | 21 | 14 | 3 | 2 | 6 | 1 | 0 | 1 | 3 | 7 | 18 |
Cytoplasm | 100 | 154 | 68 | 102 | 227 | 203 | 230 | 278 | 265 | 339 | 205 | 282 | 120 | 165 | 214 | 103 | 88 | 112 | 23 | 26 | 17 | 22 | 26 | 35 |
Endoplasmic Reticulum | 17 | 23 | 1 | 5 | 9 | 8 | 3 | 9 | 6 | 12 | 2 | 6 | 5 | 3 | 0 | 13 | 18 | 17 | 9 | 16 | 10 | 21 | 32 | 79 |
Endosome | 0 | 5 | 0 | 0 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 1 | 0 | 1 | 8 | 4 | 9 | 1 | 9 | 3 | 1 | 1 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 7 | 6 | 3 | 0 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 10 | 1 | 1 | 1 | 1 | 5 | 2 | 3 | 10 | 7 | 12 | 6 | 0 | 0 | 0 | 4 | 0 | 7 | 8 | 5 | 11 | 6 | 5 | 8 |
Nucleus | 11 | 16 | 4 | 19 | 9 | 12 | 4 | 6 | 4 | 4 | 1 | 1 | 2 | 1 | 0 | 204 | 130 | 118 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 19 | 81 | 26 | 42 | 81 | 46 | 33 | 26 | 31 | 42 | 18 | 39 | 0 | 0 | 5 | 342 | 229 | 248 | 143 | 227 | 100 | 93 | 109 | 125 |
Unique Cell Count | 150 | 262 | 94 | 141 | 304 | 248 | 265 | 311 | 301 | 389 | 230 | 318 | 130 | 178 | 219 | 542 | 360 | 423 | 203 | 315 | 160 | 168 | 210 | 312 |
Labelled Cell Count | 159 | 286 | 102 | 169 | 336 | 280 | 287 | 341 | 337 | 436 | 268 | 361 | 138 | 200 | 240 | 686 | 478 | 524 | 203 | 315 | 160 | 168 | 210 | 312 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 6.9 | 6.2 | 6.8 | 6.9 | 6.2 | 5.9 | 6.5 | 5.7 | 6.4 | 5.7 | 6.2 | 6.5 | 6.6 | 6.8 | 9.2 | 9.7 | 9.8 | 5.5 | 6.8 | 7.4 |
Std Deviation (1e-4) | 0.9 | 1.6 | 1.4 | 2.1 | 1.9 | 1.7 | 1.5 | 1.7 | 1.3 | 1.8 | 1.3 | 1.3 | 1.3 | 1.6 | 1.8 | 2.5 | 2.3 | 2.6 | 1.6 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.17 | 0.01 | -0.07 | 0.08 | -0.12 | 0.05 | -0.12 | 0.01 | 0.07 | 0.09 | 0.14 | 0.58 | 0.66 | 0.67 | -0.15 | 0.14 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.5 | 1.9 | 3.2 | 4.1 | 3.7 | 3.5 | 3.8 | 3.9 | 4.0 | 4.6 | 6.9 | -10.8 | -8.7 | -8.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.4 | 6.0 | 4.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.4 | -0.2 | -1.8 | -3.4 | -4.9 | -4.2 | -4.2 | -4.7 | -3.6 | -6.4 | -7.4 | -6.9 | 6.4 | 6.3 | 5.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1851 | 2.0038 | 2.2277 | 1.925 | 2.1534 | 2.055 | 0.2296 | 1.2749 | 1.0248 | 0.6626 | 0.8552 | 1.0417 | 0.7485 | 0.7416 | 0.4853 | 1.4522 | 0.3587 | 0.3359 |
Actin | 0.0182 | 0.0024 | 0.0085 | 0.0086 | 0.0058 | 0.0017 | 0.0167 | 0.0003 | 0.0012 | 0.0003 | 0.0017 | 0.0004 | 0.0507 | 0.0079 | 0.0226 | 0.0135 | 0.0016 | 0.0066 |
Bud | 0.0019 | 0.0031 | 0.0026 | 0.0046 | 0.0029 | 0.0014 | 0.0019 | 0.0027 | 0.009 | 0.002 | 0.0061 | 0.0009 | 0.0026 | 0.0023 | 0.0031 | 0.0036 | 0.0034 | 0.0008 |
Bud Neck | 0.0019 | 0.0022 | 0.0021 | 0.01 | 0.0058 | 0.0092 | 0.0045 | 0.004 | 0.0101 | 0.0019 | 0.0011 | 0.0093 | 0.008 | 0.0016 | 0.0022 | 0.0045 | 0.0025 | 0.0198 |
Bud Periphery | 0.0037 | 0.0079 | 0.0088 | 0.0131 | 0.0081 | 0.0043 | 0.0044 | 0.0046 | 0.0192 | 0.0095 | 0.0075 | 0.0019 | 0.0073 | 0.0039 | 0.0075 | 0.0074 | 0.0114 | 0.0053 |
Bud Site | 0.0141 | 0.0472 | 0.0319 | 0.0171 | 0.011 | 0.0024 | 0.0166 | 0.0322 | 0.048 | 0.0046 | 0.0028 | 0.0013 | 0.025 | 0.06 | 0.0103 | 0.0092 | 0.0042 | 0.0013 |
Cell Periphery | 0.0081 | 0.0071 | 0.0049 | 0.0037 | 0.0044 | 0.0033 | 0.0036 | 0.0058 | 0.0041 | 0.0063 | 0.002 | 0.0018 | 0.0049 | 0.0061 | 0.0039 | 0.0014 | 0.0029 | 0.0028 |
Cytoplasm | 0.2806 | 0.3635 | 0.403 | 0.3006 | 0.384 | 0.4338 | 0.2947 | 0.3594 | 0.3761 | 0.4512 | 0.3884 | 0.418 | 0.3928 | 0.4216 | 0.4662 | 0.4028 | 0.4905 | 0.5098 |
Cytoplasmic Foci | 0.0579 | 0.0272 | 0.0283 | 0.0537 | 0.0429 | 0.0301 | 0.0457 | 0.0201 | 0.0359 | 0.0487 | 0.0427 | 0.0425 | 0.0552 | 0.0259 | 0.0287 | 0.0517 | 0.0396 | 0.0416 |
Eisosomes | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0008 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.013 | 0.0045 | 0.0076 | 0.0058 | 0.0088 | 0.0048 | 0.008 | 0.0089 | 0.0086 | 0.0063 | 0.0048 | 0.0057 | 0.0124 | 0.0098 | 0.0086 | 0.0099 | 0.0071 | 0.006 |
Endosome | 0.0763 | 0.0346 | 0.0599 | 0.1106 | 0.0613 | 0.0555 | 0.0464 | 0.0345 | 0.0599 | 0.0532 | 0.076 | 0.0619 | 0.0928 | 0.0362 | 0.0481 | 0.096 | 0.0788 | 0.0501 |
Golgi | 0.0102 | 0.0044 | 0.0101 | 0.0132 | 0.0127 | 0.0046 | 0.0027 | 0.0011 | 0.0017 | 0.0014 | 0.0019 | 0.0014 | 0.0156 | 0.0041 | 0.0048 | 0.0196 | 0.007 | 0.0074 |
Lipid Particles | 0.0226 | 0.0025 | 0.0071 | 0.006 | 0.0182 | 0.0084 | 0.0097 | 0.0039 | 0.0043 | 0.0027 | 0.0084 | 0.0018 | 0.0196 | 0.0084 | 0.004 | 0.0102 | 0.0198 | 0.0103 |
Mitochondria | 0.0082 | 0.0033 | 0.0155 | 0.0067 | 0.069 | 0.0076 | 0.0031 | 0.0009 | 0.0014 | 0.0013 | 0.0016 | 0.0008 | 0.0277 | 0.0013 | 0.0071 | 0.0514 | 0.0015 | 0.0085 |
None | 0.3824 | 0.4284 | 0.3424 | 0.3508 | 0.2409 | 0.3367 | 0.4496 | 0.379 | 0.3232 | 0.3215 | 0.3241 | 0.2976 | 0.1791 | 0.3382 | 0.3125 | 0.1836 | 0.2172 | 0.2603 |
Nuclear Periphery | 0.0108 | 0.0009 | 0.0022 | 0.0019 | 0.0021 | 0.0021 | 0.0072 | 0.002 | 0.0019 | 0.0016 | 0.0025 | 0.0015 | 0.0119 | 0.0017 | 0.0016 | 0.003 | 0.0012 | 0.0038 |
Nucleolus | 0.002 | 0.0012 | 0.001 | 0.0032 | 0.0007 | 0.0011 | 0.0022 | 0.0017 | 0.0023 | 0.0009 | 0.0041 | 0.001 | 0.0014 | 0.0008 | 0.0005 | 0.0011 | 0.0005 | 0.0004 |
Nucleus | 0.0124 | 0.0032 | 0.0032 | 0.0054 | 0.002 | 0.004 | 0.0052 | 0.0069 | 0.0055 | 0.0035 | 0.0056 | 0.0046 | 0.0178 | 0.0031 | 0.0022 | 0.0354 | 0.0032 | 0.0021 |
Peroxisomes | 0.0118 | 0.0012 | 0.007 | 0.0067 | 0.0236 | 0.0031 | 0.0036 | 0.0004 | 0.0009 | 0.0008 | 0.0043 | 0.0007 | 0.01 | 0.0016 | 0.0072 | 0.0023 | 0.0051 | 0.003 |
Punctate Nuclear | 0.0086 | 0.0022 | 0.0023 | 0.0018 | 0.0009 | 0.0043 | 0.0039 | 0.0025 | 0.003 | 0.0018 | 0.0134 | 0.002 | 0.0033 | 0.0014 | 0.0008 | 0.0028 | 0.001 | 0.0022 |
Vacuole | 0.0513 | 0.0514 | 0.0491 | 0.0737 | 0.0909 | 0.0786 | 0.0672 | 0.1263 | 0.0813 | 0.0776 | 0.0965 | 0.1414 | 0.0565 | 0.062 | 0.0555 | 0.087 | 0.0989 | 0.0558 |
Vacuole Periphery | 0.0033 | 0.0013 | 0.0023 | 0.0027 | 0.0036 | 0.0028 | 0.003 | 0.0026 | 0.0024 | 0.0028 | 0.0046 | 0.0036 | 0.0045 | 0.0018 | 0.0023 | 0.0031 | 0.0026 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 66.7341 | 47.3606 | 27.0336 | 32.9979 | 43.5921 | 75.3525 | 51.1094 | 45.6831 | 45.5836 | 58.1208 |
Translational Efficiency | 1.0423 | 0.9016 | 0.713 | 0.9821 | 1.0338 | 0.959 | 0.8377 | 0.7958 | 0.7791 | 0.8855 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1570 | 1196 | 2027 | 177 | 2155 | 2089 | 304 | 1171 | 3725 | 3285 | 2331 | 1348 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 744.71 | 849.87 | 871.24 | 1169.39 | 790.21 | 818.98 | 1163.45 | 1109.69 | 771.03 | 830.23 | 909.35 | 1117.53 |
Standard Deviation | 112.48 | 157.41 | 114.22 | 198.00 | 110.07 | 123.57 | 221.59 | 218.41 | 113.34 | 137.67 | 165.63 | 216.78 |
Intensity Change Log 2 | — | 0.190563 | 0.226391 | 0.651006 | — | 0.051592 | 0.558101 | 0.489849 | — | 0.120691 | 0.406646 | 0.570288 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001220 | 0.001796 | 0.001069 | 0.014966 | 0.000547 | 0.001611 | 0.007587 | 0.017032 | 0.000831 | 0.001678 | 0.001919 | 0.016761 |
Bud Neck | 0.061883 | 0.040993 | 0.037895 | 0.036874 | 0.045951 | 0.063604 | 0.033705 | 0.052510 | 0.052666 | 0.055371 | 0.037349 | 0.050457 |
Bud Site | 0.028341 | 0.045066 | 0.032252 | 0.109334* | 0.019322 | 0.051885 | 0.083255 | 0.097058 | 0.023123 | 0.049402 | 0.038904 | 0.098670 |
Cell Periphery | 0.004099 | 0.002402 | 0.001315 | 0.071974 | 0.003854 | 0.005154 | 0.087021 | 0.286342* | 0.003957 | 0.004152 | 0.012493 | 0.258194* |
Cytoplasm | 0.187239* | 0.200986* | 0.260746* | 0.048301 | 0.112671* | 0.202256* | 0.128397* | 0.152127* | 0.144100* | 0.201794* | 0.243485* | 0.138494* |
Cytoplasmic Foci | 0.053147 | 0.078315 | 0.035358 | 0.023572 | 0.019376 | 0.057450 | 0.013048 | 0.008844 | 0.033610 | 0.065047 | 0.032448 | 0.010778 |
Eisosomes | 0.000773 | 0.000310 | 0.000278 | 0.001047 | 0.000333 | 0.001056 | 0.000941 | 0.005351 | 0.000519 | 0.000784 | 0.000364 | 0.004786 |
Endoplasmic Reticulum | 0.004632 | 0.009297 | 0.015269 | 0.029198 | 0.006809 | 0.012317 | 0.078775 | 0.032239 | 0.005892 | 0.011218 | 0.023552 | 0.031840 |
Endosome | 0.050776 | 0.076913 | 0.031520 | 0.053044 | 0.038148 | 0.072820 | 0.034311 | 0.010853 | 0.043471 | 0.074310 | 0.031884 | 0.016393 |
Golgi | 0.003895 | 0.006510 | 0.000716 | 0.010103 | 0.001249 | 0.007270 | 0.003513 | 0.005454 | 0.002364 | 0.006993 | 0.001081 | 0.006064 |
Lipid Particles | 0.005001 | 0.005060 | 0.007107 | 0.016472 | 0.002559 | 0.007364 | 0.032954 | 0.025736 | 0.003588 | 0.006525 | 0.010478 | 0.024519 |
Mitochondria | 0.002655 | 0.003214 | 0.000833 | 0.000213 | 0.001507 | 0.005432 | 0.000438 | 0.001294 | 0.001991 | 0.004624 | 0.000781 | 0.001152 |
Mitotic Spindle | 0.000954 | 0.001974 | 0.004233 | 0.000342 | 0.000198 | 0.002123 | 0.000181 | 0.002323 | 0.000516 | 0.002069 | 0.003704 | 0.002063 |
None | 0.000816 | 0.002144 | 0.001132 | 0.000244 | 0.000105 | 0.000367 | 0.000300 | 0.000386 | 0.000405 | 0.001014 | 0.001024 | 0.000367 |
Nuclear Periphery | 0.000086 | 0.000162 | 0.001751 | 0.000132 | 0.000062 | 0.000146 | 0.000193 | 0.000265 | 0.000073 | 0.000152 | 0.001548 | 0.000247 |
Nuclear Periphery Foci | 0.000223 | 0.000527 | 0.002463 | 0.002950 | 0.000157 | 0.000423 | 0.008893 | 0.001792 | 0.000185 | 0.000461 | 0.003301 | 0.001944 |
Nucleolus | 0.000412 | 0.001121 | 0.001198 | 0.000191 | 0.000228 | 0.000472 | 0.000142 | 0.000135 | 0.000305 | 0.000709 | 0.001060 | 0.000142 |
Nucleus | 0.012886 | 0.013219 | 0.146155* | 0.005585 | 0.007130 | 0.016104 | 0.004758 | 0.007264 | 0.009556 | 0.015053 | 0.127714 | 0.007044 |
Peroxisomes | 0.003965 | 0.004135 | 0.002298 | 0.003393 | 0.000892 | 0.004190 | 0.001445 | 0.002737 | 0.002187 | 0.004170 | 0.002187 | 0.002823 |
Vacuole | 0.571408* | 0.493542* | 0.414484* | 0.554805* | 0.733777* | 0.478553* | 0.475874* | 0.287328* | 0.665342* | 0.484010* | 0.422490* | 0.322449* |
Vacuole Periphery | 0.005590 | 0.012313 | 0.001929 | 0.017259 | 0.005124 | 0.009404 | 0.004266 | 0.002929 | 0.005320 | 0.010463 | 0.002234 | 0.004811 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.90 | 0.39 | -2.23 | -2.13 | -2.27 | -3.86 | -5.54 | -9.74 | -9.08 | -4.41 | -2.82 | -3.69 | -9.57 | -9.02 | -8.89 |
Bud Neck | 5.42 | 7.15 | 4.67 | 1.11 | 0.42 | -5.32 | 2.71 | -1.44 | 3.38 | -3.56 | -1.02 | 6.47 | 1.26 | 2.08 | -4.06 |
Bud Site | -5.32 | -1.03 | -5.57 | -4.31 | -5.34 | -11.99 | -6.71 | -14.38 | -7.84 | -1.16 | -12.66 | -6.03 | -14.97 | -9.34 | -11.11 |
Cell Periphery | 3.96 | 7.78 | -5.63 | -5.79 | -5.90 | -2.01 | -8.59 | -33.56* | -33.33* | -15.53 | -0.42 | -5.92 | -32.83 | -32.77* | -31.24* |
Cytoplasm | -1.59 | -9.33 | 11.79 | 12.26 | 18.70 | -13.50 | -1.50 | -6.22 | 5.78 | -2.23 | -10.74 | -16.45 | 0.04 | 8.58 | 13.63 |
Cytoplasmic Foci | -5.49 | 5.95 | 5.30 | 9.02 | 2.19 | -14.99 | 3.09 | 6.31 | 19.00 | 2.01 | -13.12 | 0.85 | 12.54 | 23.51 | 12.17 |
Eisosomes | 5.02 | 5.52 | -0.59 | -1.98 | -2.12 | -4.63 | -3.35 | -9.00 | -7.43 | -7.57 | -2.51 | 2.63 | -8.71 | -8.03 | -9.07 |
Endoplasmic Reticulum | -4.22 | -10.40 | -4.82 | -3.77 | -2.61 | -4.71 | -8.23 | -10.20 | -7.43 | 5.18 | -6.32 | -11.83 | -11.51 | -8.70 | -3.10 |
Endosome | -6.53 | 7.68 | 0.24 | 4.26 | -3.39 | -11.59 | 1.11 | 14.78 | 22.20 | 6.29 | -12.78 | 6.83 | 15.47 | 24.59 | 7.86 |
Golgi | -2.53 | 6.42 | -2.50 | -1.18 | -4.15 | -7.25 | -4.02 | -3.48 | 1.41 | -1.47 | -7.07 | 4.85 | -3.24 | 1.00 | -4.55 |
Lipid Particles | -0.11 | -3.37 | -2.31 | -2.29 | -1.86 | -10.44 | -6.68 | -10.20 | -7.87 | 1.60 | -8.00 | -9.12 | -10.04 | -8.50 | -6.28 |
Mitochondria | -0.66 | 5.05 | 6.82 | 4.02 | 6.99 | -5.16 | 2.62 | 0.45 | 5.34 | -1.92 | -4.64 | 4.68 | 2.07 | 5.74 | -1.03 |
Mitotic Spindle | -0.94 | -3.07 | 0.79 | 1.49 | 3.81 | -3.42 | 0.06 | -2.76 | -0.80 | -2.80 | -2.88 | -3.72 | -2.40 | -0.64 | 0.91 |
None | -1.61 | -0.77 | 1.30 | 2.28 | 2.33 | -2.86 | -0.71 | -1.69 | -0.12 | -0.29 | -1.93 | -2.24 | 0.13 | 1.98 | 2.29 |
Nuclear Periphery | -2.98 | -8.75 | -3.52 | -0.60 | 8.17 | -5.82 | -6.31 | -4.53 | -2.62 | -1.36 | -6.04 | -8.90 | -4.66 | -2.57 | 7.60 |
Nuclear Periphery Foci | -5.20 | -12.90 | -5.73 | -5.08 | -1.18 | -5.73 | -4.62 | -7.58 | -6.31 | 3.72 | -7.56 | -10.52 | -8.89 | -7.47 | 3.56 |
Nucleolus | -1.65 | -5.77 | 1.17 | 1.94 | 5.90 | -3.72 | 3.73 | 4.07 | 4.98 | 0.39 | -2.45 | -7.06 | 4.29 | 3.41 | 8.64 |
Nucleus | -0.55 | -30.04 | -0.18 | 0.00 | 21.47 | -7.93 | 1.41 | -2.23 | 4.69 | -2.77 | -6.11 | -29.98 | -0.44 | 3.97 | 29.14 |
Peroxisomes | -0.17 | 2.83 | 0.54 | 0.63 | -0.67 | -8.60 | -1.63 | -5.10 | 3.21 | -2.77 | -5.35 | 0.19 | -1.21 | 3.39 | -1.36 |
Vacuole | 6.18 | 14.94 | 0.46 | -2.81 | -6.43 | 27.13* | 14.59 | 46.94* | 18.08 | 10.16 | 23.56 | 30.53 | 37.76 | 16.62 | 9.58 |
Vacuole Periphery | -6.56 | 8.95 | -3.20 | -1.03 | -4.44 | -7.37 | 1.27 | 4.05 | 9.11 | 1.45 | -9.75 | 11.53 | 1.23 | 7.68 | -3.86 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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