Standard name
Human Ortholog
Description Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0 0 0 0 0 0 0 0 0.06
Cytoplasm 0.67 0.59 0.72 0.72 0.75 0.82 0.87 0.89 0.88 0.87 0.89 0.89 0.92 0.93 0.98 0.19 0.24 0.26 0.11 0.08 0.11 0.14 0.13 0.11
Endoplasmic Reticulum 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.06 0.13 0.16 0.26
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0.07 0.06 0 0.13 0 0.05 0 0 0 0 0 0 0 0 0 0.38 0.36 0.28 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.31 0.28 0.3 0.27 0.19 0.12 0.08 0.1 0.11 0.08 0.12 0 0 0 0.63 0.64 0.59 0.71 0.72 0.63 0.55 0.52 0.4
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 1 1 4 1 1 3 0 1 2 5 5 2 1 1 3 3 3 9 8 11
Bud 1 4 1 0 0 2 6 10 13 16 19 12 0 1 0 0 0 0 3 9 1 2 4 5
Bud Neck 0 0 0 0 0 0 0 1 0 2 0 0 1 1 1 0 0 0 2 9 3 6 10 18
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 3 0 4 6 7 9 10 8 5 21 14 3 2 6 1 0 1 3 7 18
Cytoplasm 100 154 68 102 227 203 230 278 265 339 205 282 120 165 214 103 88 112 23 26 17 22 26 35
Endoplasmic Reticulum 17 23 1 5 9 8 3 9 6 12 2 6 5 3 0 13 18 17 9 16 10 21 32 79
Endosome 0 5 0 0 4 0 1 1 0 0 0 4 1 0 1 8 4 9 1 9 3 1 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 7 6 3 0 1 0 0 1 1
Mitochondria 10 1 1 1 1 5 2 3 10 7 12 6 0 0 0 4 0 7 8 5 11 6 5 8
Nucleus 11 16 4 19 9 12 4 6 4 4 1 1 2 1 0 204 130 118 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 2 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 1 1 0 0 0 1 0 0 1 3 0 0 0 2 1 2 0 0 0 0
Vac/Vac Membrane 19 81 26 42 81 46 33 26 31 42 18 39 0 0 5 342 229 248 143 227 100 93 109 125
Unique Cell Count 150 262 94 141 304 248 265 311 301 389 230 318 130 178 219 542 360 423 203 315 160 168 210 312
Labelled Cell Count 159 286 102 169 336 280 287 341 337 436 268 361 138 200 240 686 478 524 203 315 160 168 210 312


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.9 6.2 6.8 6.9 6.2 5.9 6.5 5.7 6.4 5.7 6.2 6.5 6.6 6.8 9.2 9.7 9.8 5.5 6.8 7.4
Std Deviation (1e-4) 0.9 1.6 1.4 2.1 1.9 1.7 1.5 1.7 1.3 1.8 1.3 1.3 1.3 1.6 1.8 2.5 2.3 2.6 1.6 1.8 1.7
Intensity Change (Log2) – – – 0.14 0.17 0.01 -0.07 0.08 -0.12 0.05 -0.12 0.01 0.07 0.09 0.14 0.58 0.66 0.67 -0.15 0.14 0.27

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 2.4 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 3.5 0 0 0 0
Cytoplasm 0 0.5 1.9 3.2 4.1 3.7 3.5 3.8 3.9 4.0 4.6 6.9 -10.8 -8.7 -8.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 2.3 0 0 0 0 0 0 0 0 0 0 0 6.4 6.0 4.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 -0.2 -1.8 -3.4 -4.9 -4.2 -4.2 -4.7 -3.6 -6.4 -7.4 -6.9 6.4 6.3 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1851 2.0038 2.2277 1.925 2.1534 2.055 0.2296 1.2749 1.0248 0.6626 0.8552 1.0417 0.7485 0.7416 0.4853 1.4522 0.3587 0.3359
Actin 0.0182 0.0024 0.0085 0.0086 0.0058 0.0017 0.0167 0.0003 0.0012 0.0003 0.0017 0.0004 0.0507 0.0079 0.0226 0.0135 0.0016 0.0066
Bud 0.0019 0.0031 0.0026 0.0046 0.0029 0.0014 0.0019 0.0027 0.009 0.002 0.0061 0.0009 0.0026 0.0023 0.0031 0.0036 0.0034 0.0008
Bud Neck 0.0019 0.0022 0.0021 0.01 0.0058 0.0092 0.0045 0.004 0.0101 0.0019 0.0011 0.0093 0.008 0.0016 0.0022 0.0045 0.0025 0.0198
Bud Periphery 0.0037 0.0079 0.0088 0.0131 0.0081 0.0043 0.0044 0.0046 0.0192 0.0095 0.0075 0.0019 0.0073 0.0039 0.0075 0.0074 0.0114 0.0053
Bud Site 0.0141 0.0472 0.0319 0.0171 0.011 0.0024 0.0166 0.0322 0.048 0.0046 0.0028 0.0013 0.025 0.06 0.0103 0.0092 0.0042 0.0013
Cell Periphery 0.0081 0.0071 0.0049 0.0037 0.0044 0.0033 0.0036 0.0058 0.0041 0.0063 0.002 0.0018 0.0049 0.0061 0.0039 0.0014 0.0029 0.0028
Cytoplasm 0.2806 0.3635 0.403 0.3006 0.384 0.4338 0.2947 0.3594 0.3761 0.4512 0.3884 0.418 0.3928 0.4216 0.4662 0.4028 0.4905 0.5098
Cytoplasmic Foci 0.0579 0.0272 0.0283 0.0537 0.0429 0.0301 0.0457 0.0201 0.0359 0.0487 0.0427 0.0425 0.0552 0.0259 0.0287 0.0517 0.0396 0.0416
Eisosomes 0.0007 0.0004 0.0002 0.0002 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0 0.0008 0.0002 0.0002 0.0005 0.0001 0.0001
Endoplasmic Reticulum 0.013 0.0045 0.0076 0.0058 0.0088 0.0048 0.008 0.0089 0.0086 0.0063 0.0048 0.0057 0.0124 0.0098 0.0086 0.0099 0.0071 0.006
Endosome 0.0763 0.0346 0.0599 0.1106 0.0613 0.0555 0.0464 0.0345 0.0599 0.0532 0.076 0.0619 0.0928 0.0362 0.0481 0.096 0.0788 0.0501
Golgi 0.0102 0.0044 0.0101 0.0132 0.0127 0.0046 0.0027 0.0011 0.0017 0.0014 0.0019 0.0014 0.0156 0.0041 0.0048 0.0196 0.007 0.0074
Lipid Particles 0.0226 0.0025 0.0071 0.006 0.0182 0.0084 0.0097 0.0039 0.0043 0.0027 0.0084 0.0018 0.0196 0.0084 0.004 0.0102 0.0198 0.0103
Mitochondria 0.0082 0.0033 0.0155 0.0067 0.069 0.0076 0.0031 0.0009 0.0014 0.0013 0.0016 0.0008 0.0277 0.0013 0.0071 0.0514 0.0015 0.0085
None 0.3824 0.4284 0.3424 0.3508 0.2409 0.3367 0.4496 0.379 0.3232 0.3215 0.3241 0.2976 0.1791 0.3382 0.3125 0.1836 0.2172 0.2603
Nuclear Periphery 0.0108 0.0009 0.0022 0.0019 0.0021 0.0021 0.0072 0.002 0.0019 0.0016 0.0025 0.0015 0.0119 0.0017 0.0016 0.003 0.0012 0.0038
Nucleolus 0.002 0.0012 0.001 0.0032 0.0007 0.0011 0.0022 0.0017 0.0023 0.0009 0.0041 0.001 0.0014 0.0008 0.0005 0.0011 0.0005 0.0004
Nucleus 0.0124 0.0032 0.0032 0.0054 0.002 0.004 0.0052 0.0069 0.0055 0.0035 0.0056 0.0046 0.0178 0.0031 0.0022 0.0354 0.0032 0.0021
Peroxisomes 0.0118 0.0012 0.007 0.0067 0.0236 0.0031 0.0036 0.0004 0.0009 0.0008 0.0043 0.0007 0.01 0.0016 0.0072 0.0023 0.0051 0.003
Punctate Nuclear 0.0086 0.0022 0.0023 0.0018 0.0009 0.0043 0.0039 0.0025 0.003 0.0018 0.0134 0.002 0.0033 0.0014 0.0008 0.0028 0.001 0.0022
Vacuole 0.0513 0.0514 0.0491 0.0737 0.0909 0.0786 0.0672 0.1263 0.0813 0.0776 0.0965 0.1414 0.0565 0.062 0.0555 0.087 0.0989 0.0558
Vacuole Periphery 0.0033 0.0013 0.0023 0.0027 0.0036 0.0028 0.003 0.0026 0.0024 0.0028 0.0046 0.0036 0.0045 0.0018 0.0023 0.0031 0.0026 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 66.7341 47.3606 27.0336 32.9979 43.5921 75.3525 51.1094 45.6831 45.5836 58.1208
Translational Efficiency 1.0423 0.9016 0.713 0.9821 1.0338 0.959 0.8377 0.7958 0.7791 0.8855

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1570 1196 2027 177 2155 2089 304 1171 3725 3285 2331 1348

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 744.71 849.87 871.24 1169.39 790.21 818.98 1163.45 1109.69 771.03 830.23 909.35 1117.53
Standard Deviation 112.48 157.41 114.22 198.00 110.07 123.57 221.59 218.41 113.34 137.67 165.63 216.78
Intensity Change Log 2 — 0.190563 0.226391 0.651006 — 0.051592 0.558101 0.489849 — 0.120691 0.406646 0.570288

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001220 0.001796 0.001069 0.014966 0.000547 0.001611 0.007587 0.017032 0.000831 0.001678 0.001919 0.016761
Bud Neck 0.061883 0.040993 0.037895 0.036874 0.045951 0.063604 0.033705 0.052510 0.052666 0.055371 0.037349 0.050457
Bud Site 0.028341 0.045066 0.032252 0.109334 0.019322 0.051885 0.083255 0.097058 0.023123 0.049402 0.038904 0.098670
Cell Periphery 0.004099 0.002402 0.001315 0.071974 0.003854 0.005154 0.087021 0.286342 0.003957 0.004152 0.012493 0.258194
Cytoplasm 0.187239 0.200986 0.260746 0.048301 0.112671 0.202256 0.128397 0.152127 0.144100 0.201794 0.243485 0.138494
Cytoplasmic Foci 0.053147 0.078315 0.035358 0.023572 0.019376 0.057450 0.013048 0.008844 0.033610 0.065047 0.032448 0.010778
Eisosomes 0.000773 0.000310 0.000278 0.001047 0.000333 0.001056 0.000941 0.005351 0.000519 0.000784 0.000364 0.004786
Endoplasmic Reticulum 0.004632 0.009297 0.015269 0.029198 0.006809 0.012317 0.078775 0.032239 0.005892 0.011218 0.023552 0.031840
Endosome 0.050776 0.076913 0.031520 0.053044 0.038148 0.072820 0.034311 0.010853 0.043471 0.074310 0.031884 0.016393
Golgi 0.003895 0.006510 0.000716 0.010103 0.001249 0.007270 0.003513 0.005454 0.002364 0.006993 0.001081 0.006064
Lipid Particles 0.005001 0.005060 0.007107 0.016472 0.002559 0.007364 0.032954 0.025736 0.003588 0.006525 0.010478 0.024519
Mitochondria 0.002655 0.003214 0.000833 0.000213 0.001507 0.005432 0.000438 0.001294 0.001991 0.004624 0.000781 0.001152
Mitotic Spindle 0.000954 0.001974 0.004233 0.000342 0.000198 0.002123 0.000181 0.002323 0.000516 0.002069 0.003704 0.002063
None 0.000816 0.002144 0.001132 0.000244 0.000105 0.000367 0.000300 0.000386 0.000405 0.001014 0.001024 0.000367
Nuclear Periphery 0.000086 0.000162 0.001751 0.000132 0.000062 0.000146 0.000193 0.000265 0.000073 0.000152 0.001548 0.000247
Nuclear Periphery Foci 0.000223 0.000527 0.002463 0.002950 0.000157 0.000423 0.008893 0.001792 0.000185 0.000461 0.003301 0.001944
Nucleolus 0.000412 0.001121 0.001198 0.000191 0.000228 0.000472 0.000142 0.000135 0.000305 0.000709 0.001060 0.000142
Nucleus 0.012886 0.013219 0.146155 0.005585 0.007130 0.016104 0.004758 0.007264 0.009556 0.015053 0.127714 0.007044
Peroxisomes 0.003965 0.004135 0.002298 0.003393 0.000892 0.004190 0.001445 0.002737 0.002187 0.004170 0.002187 0.002823
Vacuole 0.571408 0.493542 0.414484 0.554805 0.733777 0.478553 0.475874 0.287328 0.665342 0.484010 0.422490 0.322449
Vacuole Periphery 0.005590 0.012313 0.001929 0.017259 0.005124 0.009404 0.004266 0.002929 0.005320 0.010463 0.002234 0.004811

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.90 0.39 -2.23 -2.13 -2.27 -3.86 -5.54 -9.74 -9.08 -4.41 -2.82 -3.69 -9.57 -9.02 -8.89
Bud Neck 5.42 7.15 4.67 1.11 0.42 -5.32 2.71 -1.44 3.38 -3.56 -1.02 6.47 1.26 2.08 -4.06
Bud Site -5.32 -1.03 -5.57 -4.31 -5.34 -11.99 -6.71 -14.38 -7.84 -1.16 -12.66 -6.03 -14.97 -9.34 -11.11
Cell Periphery 3.96 7.78 -5.63 -5.79 -5.90 -2.01 -8.59 -33.56 -33.33 -15.53 -0.42 -5.92 -32.83 -32.77 -31.24
Cytoplasm -1.59 -9.33 11.79 12.26 18.70 -13.50 -1.50 -6.22 5.78 -2.23 -10.74 -16.45 0.04 8.58 13.63
Cytoplasmic Foci -5.49 5.95 5.30 9.02 2.19 -14.99 3.09 6.31 19.00 2.01 -13.12 0.85 12.54 23.51 12.17
Eisosomes 5.02 5.52 -0.59 -1.98 -2.12 -4.63 -3.35 -9.00 -7.43 -7.57 -2.51 2.63 -8.71 -8.03 -9.07
Endoplasmic Reticulum -4.22 -10.40 -4.82 -3.77 -2.61 -4.71 -8.23 -10.20 -7.43 5.18 -6.32 -11.83 -11.51 -8.70 -3.10
Endosome -6.53 7.68 0.24 4.26 -3.39 -11.59 1.11 14.78 22.20 6.29 -12.78 6.83 15.47 24.59 7.86
Golgi -2.53 6.42 -2.50 -1.18 -4.15 -7.25 -4.02 -3.48 1.41 -1.47 -7.07 4.85 -3.24 1.00 -4.55
Lipid Particles -0.11 -3.37 -2.31 -2.29 -1.86 -10.44 -6.68 -10.20 -7.87 1.60 -8.00 -9.12 -10.04 -8.50 -6.28
Mitochondria -0.66 5.05 6.82 4.02 6.99 -5.16 2.62 0.45 5.34 -1.92 -4.64 4.68 2.07 5.74 -1.03
Mitotic Spindle -0.94 -3.07 0.79 1.49 3.81 -3.42 0.06 -2.76 -0.80 -2.80 -2.88 -3.72 -2.40 -0.64 0.91
None -1.61 -0.77 1.30 2.28 2.33 -2.86 -0.71 -1.69 -0.12 -0.29 -1.93 -2.24 0.13 1.98 2.29
Nuclear Periphery -2.98 -8.75 -3.52 -0.60 8.17 -5.82 -6.31 -4.53 -2.62 -1.36 -6.04 -8.90 -4.66 -2.57 7.60
Nuclear Periphery Foci -5.20 -12.90 -5.73 -5.08 -1.18 -5.73 -4.62 -7.58 -6.31 3.72 -7.56 -10.52 -8.89 -7.47 3.56
Nucleolus -1.65 -5.77 1.17 1.94 5.90 -3.72 3.73 4.07 4.98 0.39 -2.45 -7.06 4.29 3.41 8.64
Nucleus -0.55 -30.04 -0.18 0.00 21.47 -7.93 1.41 -2.23 4.69 -2.77 -6.11 -29.98 -0.44 3.97 29.14
Peroxisomes -0.17 2.83 0.54 0.63 -0.67 -8.60 -1.63 -5.10 3.21 -2.77 -5.35 0.19 -1.21 3.39 -1.36
Vacuole 6.18 14.94 0.46 -2.81 -6.43 27.13 14.59 46.94 18.08 10.16 23.56 30.53 37.76 16.62 9.58
Vacuole Periphery -6.56 8.95 -3.20 -1.03 -4.44 -7.37 1.27 4.05 9.11 1.45 -9.75 11.53 1.23 7.68 -3.86
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
Localization
Cell Percentages vacuole (73%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Opy2

Opy2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Opy2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available