ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 17 | 9 | 73 | 31 | 66 | 35 | 66 | – | 23 | 16 | 20 | 30 | 46 | 40 | 26 | 44 | 18 | 31 | 22 | 18 | 39 | 46 | 81 | 111 |
Bud | 5 | 3 | 10 | 6 | 21 | 14 | 23 | – | 32 | 24 | 21 | 20 | 21 | 12 | 13 | 2 | 2 | 5 | 5 | 6 | 15 | 15 | 13 | 23 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 14 | 15 | 8 | 10 |
Bud Site | 0 | 0 | 0 | 2 | 4 | 4 | 4 | – | 2 | 8 | 4 | 4 | 1 | 1 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 11 | 1 | 1 | 8 | 5 | – | 4 | 7 | 6 | 5 | 1 | 3 | 3 | 6 | 2 | 3 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 31 | 25 | 76 | 71 | 80 | 80 | 143 | – | 77 | 101 | 69 | 74 | 48 | 54 | 102 | 40 | 39 | 27 | 18 | 16 | 36 | 26 | 19 | 26 |
Endoplasmic Reticulum | 12 | 9 | 39 | 10 | 27 | 31 | 54 | – | 20 | 22 | 16 | 14 | 36 | 26 | 19 | 4 | 5 | 5 | 4 | 1 | 7 | 13 | 29 | 30 |
Endosome | 8 | 0 | 5 | 13 | 21 | 26 | 47 | – | 17 | 20 | 11 | 8 | 21 | 50 | 59 | 11 | 11 | 18 | 25 | 14 | 37 | 39 | 66 | 81 |
Golgi | 11 | 9 | 11 | 7 | 9 | 5 | 8 | – | 5 | 0 | 1 | 2 | 25 | 56 | 31 | 61 | 33 | 41 | 15 | 9 | 20 | 18 | 54 | 78 |
Mitochondria | 36 | 23 | 2 | 16 | 15 | 61 | 124 | – | 166 | 164 | 169 | 117 | 39 | 45 | 48 | 12 | 4 | 7 | 5 | 11 | 80 | 35 | 49 | 55 |
Nucleus | 1 | 3 | 1 | 1 | 2 | 1 | 3 | – | 3 | 1 | 4 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 4 | – | 0 | 1 | 0 | 0 | 1 | 2 | 5 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 0 | 0 | 0 | 4 | 5 | 4 | – | 3 | 4 | 0 | 2 | 4 | 9 | 6 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 6 | 10 |
SpindlePole | 1 | 0 | 0 | 2 | 24 | 8 | 16 | – | 6 | 10 | 9 | 12 | 3 | 5 | 8 | 11 | 2 | 10 | 3 | 0 | 2 | 3 | 4 | 5 |
Vac/Vac Membrane | 1 | 1 | 5 | 1 | 12 | 7 | 10 | – | 7 | 23 | 11 | 9 | 13 | 4 | 17 | 24 | 10 | 26 | 1 | 18 | 17 | 13 | 14 | 19 |
Unique Cell Count | 90 | 59 | 193 | 139 | 224 | 231 | 416 | 286 | 334 | 266 | 231 | 208 | 247 | 287 | 158 | 106 | 136 | 109 | 106 | 281 | 232 | 355 | 461 | |
Labelled Cell Count | 124 | 83 | 233 | 162 | 288 | 285 | 511 | 366 | 403 | 341 | 298 | 259 | 307 | 342 | 217 | 126 | 174 | 109 | 106 | 281 | 232 | 355 | 461 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 7.9 | 8.0 | 7.4 | 8.1 | 6.8 | 6.6 | – | 5.9 | 5.7 | 5.5 | 6.1 | 8.1 | 8.2 | 8.1 | 13.0 | 13.2 | 12.2 | 7.2 | 7.5 | 8.0 |
Std Deviation (1e-4) | 1.0 | 1.4 | 0.9 | 1.2 | 1.3 | 1.4 | 1.3 | – | 1.1 | 1.0 | 1.0 | 1.0 | 1.1 | 1.0 | 1.3 | 2.6 | 2.7 | 2.1 | 1.0 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.01 | -0.25 | -0.28 | – | -0.44 | -0.49 | -0.54 | -0.41 | 0.01 | 0.02 | 0.01 | 0.69 | 0.72 | 0.6 | -0.15 | -0.09 | -0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.0 | -1.8 | -5.3 | -6.0 | – | -8.0 | -9.8 | -8.0 | -5.9 | -3.4 | -5.2 | -7.6 | -2.0 | -3.7 | -2.9 |
Bud | -0.4 | 1.6 | 0.4 | 0.2 | – | 2.3 | 0.9 | 1.1 | 1.4 | 1.8 | -0.2 | -0.3 | -2.0 | 0 | -0.6 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.4 | -3.2 | -1.1 | -3.2 | – | -2.7 | -2.2 | -1.9 | -1.9 | -3.1 | -2.7 | -3.0 | -0.8 | 0 | -1.5 |
Cytoplasm | 2.1 | -0.8 | -1.0 | -1.2 | – | -2.9 | -2.1 | -3.1 | -1.6 | -3.5 | -4.0 | -0.9 | -2.8 | -0.4 | -3.8 |
Endoplasmic Reticulum | -3.3 | -2.3 | -1.9 | -2.3 | – | -4.3 | -4.7 | -4.6 | -4.4 | -0.7 | -2.8 | -4.5 | -5.0 | -3.6 | -4.3 |
Endosome | 2.7 | 2.9 | 3.4 | 3.6 | – | 1.7 | 1.8 | 0.9 | 0.5 | 3.0 | 5.6 | 5.7 | 2.0 | 2.9 | 3.7 |
Golgi | -0.3 | -0.8 | -1.9 | -2.5 | – | -2.4 | 0 | -3.5 | -2.9 | 2.2 | 4.9 | 1.9 | 7.6 | 5.9 | 6.0 |
Mitochondria | 4.2 | 2.9 | 7.3 | 8.2 | – | 12.8 | 11.4 | 13.7 | 11.3 | 5.8 | 5.8 | 5.5 | 3.1 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 4.7 | 0 | 2.8 | – | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.4 | 0.3 | 0 | – | 0 | 2.1 | 0.9 | 0.7 | 1.8 | 0 | 1.7 | 4.3 | 2.6 | 5.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.119 | 4.2343 | 3.4447 | 2.9596 | 2.5455 | 3.6202 | 2.5302 | 4.1241 | 3.5541 | 2.9724 | 2.5314 | 3.4423 | 3.083 | 4.6695 | 4.1582 | 4.3256 | 3.4357 | 4.5016 |
Actin | 0.0578 | 0.0688 | 0.0947 | 0.0588 | 0.1108 | 0.0797 | 0.0773 | 0.0965 | 0.1246 | 0.0382 | 0.094 | 0.0818 | 0.1507 | 0.0909 | 0.1304 | 0.039 | 0.0226 | 0.0947 |
Bud | 0.0033 | 0.0048 | 0.0147 | 0.0014 | 0.005 | 0.0015 | 0.0032 | 0.0089 | 0.0197 | 0.0042 | 0.0027 | 0.001 | 0.0043 | 0.0032 | 0.0163 | 0.0165 | 0.003 | 0.0015 |
Bud Neck | 0.0111 | 0.0028 | 0.036 | 0.0152 | 0.0083 | 0.0616 | 0.0067 | 0.0023 | 0.0063 | 0.0026 | 0.0065 | 0.0788 | 0.0086 | 0.0029 | 0.0083 | 0.0091 | 0.0024 | 0.0445 |
Bud Periphery | 0.0028 | 0.003 | 0.0129 | 0.0014 | 0.0033 | 0.0011 | 0.002 | 0.0032 | 0.0164 | 0.0025 | 0.0027 | 0.0006 | 0.0029 | 0.001 | 0.0089 | 0.0157 | 0.0012 | 0.001 |
Bud Site | 0.0188 | 0.0748 | 0.0457 | 0.0041 | 0.0056 | 0.0024 | 0.0193 | 0.1066 | 0.019 | 0.0025 | 0.0049 | 0.0014 | 0.0173 | 0.0657 | 0.0292 | 0.0036 | 0.0022 | 0.0017 |
Cell Periphery | 0.0007 | 0.0005 | 0.0011 | 0.0009 | 0.0006 | 0.0003 | 0.0005 | 0.0003 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0002 |
Cytoplasm | 0.1108 | 0.1789 | 0.0737 | 0.0157 | 0.0388 | 0.1089 | 0.1017 | 0.1315 | 0.14 | 0.1463 | 0.0252 | 0.077 | 0.0798 | 0.204 | 0.1366 | 0.1696 | 0.1222 | 0.084 |
Cytoplasmic Foci | 0.2007 | 0.2395 | 0.2029 | 0.1761 | 0.251 | 0.1821 | 0.1911 | 0.2406 | 0.1621 | 0.1487 | 0.1651 | 0.2016 | 0.1868 | 0.218 | 0.1743 | 0.206 | 0.1871 | 0.1987 |
Eisosomes | 0.0005 | 0.0001 | 0.0002 | 0.0104 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0001 | 0 | 0.0004 |
Endoplasmic Reticulum | 0.0114 | 0.0049 | 0.0049 | 0.0039 | 0.0019 | 0.0079 | 0.0057 | 0.001 | 0.01 | 0.004 | 0.0035 | 0.0013 | 0.0077 | 0.0024 | 0.0035 | 0.0016 | 0.0092 | 0.0016 |
Endosome | 0.3321 | 0.239 | 0.2616 | 0.3117 | 0.2396 | 0.2385 | 0.2699 | 0.2093 | 0.2392 | 0.3549 | 0.3713 | 0.2327 | 0.2376 | 0.2418 | 0.2565 | 0.3029 | 0.4281 | 0.2664 |
Golgi | 0.1631 | 0.1432 | 0.1724 | 0.1389 | 0.2328 | 0.2249 | 0.2095 | 0.1445 | 0.1952 | 0.2114 | 0.2355 | 0.2763 | 0.1794 | 0.1257 | 0.1717 | 0.1614 | 0.1924 | 0.2513 |
Lipid Particles | 0.0233 | 0.0049 | 0.004 | 0.0294 | 0.0208 | 0.009 | 0.0095 | 0.0024 | 0.0027 | 0.0091 | 0.0038 | 0.0043 | 0.0249 | 0.0027 | 0.0053 | 0.0033 | 0.0023 | 0.0049 |
Mitochondria | 0.0173 | 0.0098 | 0.0301 | 0.1241 | 0.0117 | 0.0198 | 0.0162 | 0.0045 | 0.0243 | 0.0316 | 0.058 | 0.0074 | 0.0167 | 0.0036 | 0.0133 | 0.0031 | 0.0087 | 0.0138 |
None | 0.0077 | 0.0014 | 0.0014 | 0.0485 | 0.0017 | 0.0067 | 0.0022 | 0.0007 | 0.0048 | 0.0005 | 0.0004 | 0.0003 | 0.0162 | 0.0006 | 0.0008 | 0.0358 | 0.0007 | 0.001 |
Nuclear Periphery | 0.0057 | 0.001 | 0.0038 | 0.0066 | 0.0017 | 0.0068 | 0.0154 | 0.0003 | 0.001 | 0.001 | 0.0008 | 0.0003 | 0.0082 | 0.0007 | 0.001 | 0.0008 | 0.0037 | 0.0011 |
Nucleolus | 0.0008 | 0.0001 | 0.0003 | 0.0033 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.002 | 0.0001 | 0.0001 | 0 | 0.005 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0002 |
Nucleus | 0.0018 | 0.0006 | 0.0011 | 0.0038 | 0.0005 | 0.001 | 0.0044 | 0.0006 | 0.0012 | 0.0006 | 0.0003 | 0.0002 | 0.0043 | 0.0006 | 0.0007 | 0.0009 | 0.0012 | 0.0004 |
Peroxisomes | 0.0173 | 0.0151 | 0.0245 | 0.0242 | 0.0212 | 0.0379 | 0.0455 | 0.037 | 0.0182 | 0.0156 | 0.0135 | 0.0301 | 0.0243 | 0.0253 | 0.0294 | 0.0096 | 0.0061 | 0.0269 |
Punctate Nuclear | 0.0031 | 0.0011 | 0.0014 | 0.0128 | 0.0398 | 0.0017 | 0.0018 | 0.0006 | 0.0006 | 0.0005 | 0.0005 | 0.0006 | 0.0148 | 0.0006 | 0.0007 | 0.0009 | 0.0005 | 0.0007 |
Vacuole | 0.0079 | 0.0045 | 0.0099 | 0.0049 | 0.0029 | 0.005 | 0.0147 | 0.0076 | 0.0091 | 0.0171 | 0.0052 | 0.0025 | 0.0069 | 0.0079 | 0.0081 | 0.0149 | 0.0034 | 0.0029 |
Vacuole Periphery | 0.0022 | 0.0014 | 0.0028 | 0.004 | 0.0013 | 0.0028 | 0.003 | 0.0013 | 0.003 | 0.0083 | 0.0054 | 0.0017 | 0.0025 | 0.002 | 0.004 | 0.0049 | 0.0028 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.6853 | 17.3827 | 19.2109 | 27.3568 | 18.7744 | 16.1263 | 23.2784 | 29.5297 | 22.8579 | 29.1443 |
Translational Efficiency | 0.811 | 0.9678 | 0.9097 | 0.5343 | 0.8274 | 1.4221 | 0.8201 | 0.6025 | 0.7686 | 0.7441 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1396 | 1527 | 618 | 1059 | 1630 | 1245 | 1465 | 2257 | 3026 | 2772 | 2083 | 3316 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 776.78 | 905.26 | 1106.98 | 1080.41 | 860.81 | 981.95 | 1083.16 | 1176.07 | 822.04 | 939.70 | 1090.23 | 1145.52 |
Standard Deviation | 106.29 | 136.92 | 119.68 | 164.69 | 110.65 | 130.65 | 131.01 | 152.75 | 116.46 | 139.46 | 128.22 | 162.89 |
Intensity Change Log 2 | — | 0.220826 | 0.511051 | 0.476001 | — | 0.189955 | 0.331480 | 0.450207 | — | 0.204681 | 0.419449 | 0.462500 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.015283 | 0.012267 | 0.037515 | 0.022174 | 0.024131 | 0.023365 | 0.053724 | 0.026372 | 0.020049 | 0.017251 | 0.048915 | 0.025031 |
Bud Neck | 0.007930 | 0.002333 | 0.004877 | 0.005278 | 0.004472 | 0.005469 | 0.002505 | 0.002196 | 0.006067 | 0.003741 | 0.003208 | 0.003180 |
Bud Site | 0.091185 | 0.064849 | 0.107542* | 0.096314 | 0.078007 | 0.103428* | 0.107631* | 0.080395 | 0.084086 | 0.082176 | 0.107605* | 0.085479 |
Cell Periphery | 0.000374 | 0.000145 | 0.000221 | 0.000300 | 0.000549 | 0.000443 | 0.000196 | 0.000236 | 0.000468 | 0.000279 | 0.000203 | 0.000257 |
Cytoplasm | 0.081610 | 0.078851 | 0.044751 | 0.151570* | 0.049494 | 0.104471* | 0.095628 | 0.178482* | 0.064310 | 0.090358 | 0.080533 | 0.169888* |
Cytoplasmic Foci | 0.311621* | 0.345863* | 0.293300* | 0.231606* | 0.262273* | 0.291719* | 0.283123* | 0.219655* | 0.285039* | 0.321545* | 0.286143* | 0.223472* |
Eisosomes | 0.000259 | 0.000105 | 0.000083 | 0.000054 | 0.000516 | 0.000335 | 0.000098 | 0.000067 | 0.000398 | 0.000209 | 0.000093 | 0.000063 |
Endoplasmic Reticulum | 0.000930 | 0.000827 | 0.001444 | 0.000764 | 0.002261 | 0.000817 | 0.000687 | 0.000344 | 0.001647 | 0.000823 | 0.000912 | 0.000478 |
Endosome | 0.106320 | 0.124943 | 0.166524 | 0.177700 | 0.104089 | 0.101167 | 0.136102 | 0.121111 | 0.105118 | 0.114265 | 0.145128 | 0.139183 |
Golgi | 0.276586* | 0.288348* | 0.151197* | 0.102090 | 0.392287* | 0.292970* | 0.216604* | 0.267634* | 0.338910* | 0.290424* | 0.197199* | 0.214766* |
Lipid Particles | 0.005801 | 0.004105 | 0.007619 | 0.005858 | 0.003186 | 0.001417 | 0.005205 | 0.003122 | 0.004392 | 0.002898 | 0.005921 | 0.003996 |
Mitochondria | 0.034680 | 0.016088 | 0.001332 | 0.004520 | 0.038779 | 0.022396 | 0.003255 | 0.007541 | 0.036888 | 0.018921 | 0.002684 | 0.006576 |
Mitotic Spindle | 0.010812 | 0.021922 | 0.091143 | 0.062084 | 0.005574 | 0.006267 | 0.034435 | 0.028122 | 0.007991 | 0.014891 | 0.051259 | 0.038968 |
None | 0.000552 | 0.000181 | 0.000043 | 0.000237 | 0.000126 | 0.000079 | 0.000027 | 0.000039 | 0.000323 | 0.000135 | 0.000032 | 0.000102 |
Nuclear Periphery | 0.000027 | 0.000048 | 0.000100 | 0.000413 | 0.000027 | 0.000036 | 0.000048 | 0.000055 | 0.000027 | 0.000043 | 0.000063 | 0.000169 |
Nuclear Periphery Foci | 0.000501 | 0.000920 | 0.001890 | 0.002155 | 0.000190 | 0.000135 | 0.000856 | 0.000562 | 0.000333 | 0.000567 | 0.001163 | 0.001071 |
Nucleolus | 0.000145 | 0.000185 | 0.000037 | 0.000347 | 0.000092 | 0.000033 | 0.000017 | 0.000041 | 0.000116 | 0.000117 | 0.000023 | 0.000139 |
Nucleus | 0.000497 | 0.000317 | 0.000350 | 0.001599 | 0.000631 | 0.000875 | 0.000192 | 0.000214 | 0.000569 | 0.000568 | 0.000239 | 0.000656 |
Peroxisomes | 0.038555 | 0.015444 | 0.051713 | 0.027382 | 0.017043 | 0.024399 | 0.032186 | 0.026628 | 0.026967 | 0.019466 | 0.037979 | 0.026869 |
Vacuole | 0.008869 | 0.012775 | 0.035397 | 0.099684 | 0.006719 | 0.011362 | 0.023511 | 0.029738 | 0.007711 | 0.012141 | 0.027037 | 0.052076 |
Vacuole Periphery | 0.007462 | 0.009481 | 0.002922 | 0.007871 | 0.009554 | 0.008817 | 0.003971 | 0.007448 | 0.008589 | 0.009183 | 0.003660 | 0.007583 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.41 | -4.63 | -1.74 | -2.79 | 3.10 | 0.23 | -6.48 | -0.52 | -0.78 | 6.37 | 1.44 | -8.50 | -1.89 | -3.36 | 7.11 |
Bud Neck | 7.43 | 3.61 | 2.74 | -6.42 | -1.06 | -1.42 | 3.61 | 4.31 | 6.25 | 1.71 | 4.67 | 6.06 | 5.95 | 1.33 | -0.17 |
Bud Site | 4.53 | -2.05 | -0.71 | -5.34 | 1.48 | -4.02 | -5.21 | -0.62 | 3.98 | 5.38 | 0.46 | -5.15 | -0.43 | -0.96 | 5.21 |
Cell Periphery | 7.26 | 3.76 | 0.97 | -1.67 | -0.71 | 1.23 | 5.21 | 3.43 | 2.47 | -0.55 | 4.11 | 6.55 | 3.40 | 0.49 | -0.86 |
Cytoplasm | 0.45 | 5.03 | -9.52 | -9.92 | -12.81 | -10.27 | -9.81 | -22.28 | -11.38 | -11.29 | -6.71 | -5.27 | -22.58 | -16.41 | -15.74 |
Cytoplasmic Foci | -4.90 | 2.40 | 11.40 | 16.28 | 6.76 | -4.61 | -2.80 | 8.53 | 12.28 | 10.70 | -7.68 | 0.50 | 14.95 | 22.16 | 12.59 |
Eisosomes | 5.45 | 6.44 | 7.57 | 5.45 | 3.85 | 1.10 | 2.60 | 2.80 | 8.10 | 4.83 | 2.12 | 3.48 | 3.83 | 9.21 | 6.10 |
Endoplasmic Reticulum | 0.54 | -1.71 | 0.96 | 0.24 | 2.14 | 2.25 | 2.49 | 3.07 | 3.07 | 4.33 | 2.31 | 2.01 | 3.40 | 2.93 | 3.92 |
Endosome | -3.76 | -8.00 | -8.69 | -6.00 | -0.27 | 0.67 | -6.46 | -3.74 | -4.17 | 3.38 | -2.76 | -9.76 | -8.32 | -5.49 | 2.12 |
Golgi | -1.34 | 12.67 | 22.59 | 25.62 | 5.73 | 10.53 | 21.21 | 15.71 | 3.91 | -6.16 | 7.56 | 22.32 | 20.95 | 13.75 | -2.26 |
Lipid Particles | 1.67 | -1.90 | -0.22 | -1.78 | 1.64 | 3.16 | -3.52 | -0.24 | -4.60 | 3.96 | 2.46 | -3.19 | 0.39 | -2.30 | 3.88 |
Mitochondria | 6.67 | 14.07 | 11.89 | 6.62 | -2.68 | 5.46 | 15.07 | 13.06 | 7.37 | -4.75 | 8.82 | 20.25 | 17.49 | 9.52 | -5.34 |
Mitotic Spindle | -4.59 | -10.32 | -9.84 | -7.58 | 2.79 | -0.61 | -10.73 | -10.76 | -10.37 | 1.45 | -4.93 | -14.20 | -13.98 | -10.38 | 2.78 |
None | 1.49 | 2.05 | 1.24 | -0.55 | -2.22 | 0.96 | 2.87 | 2.50 | 0.98 | -2.60 | 1.59 | 2.51 | 1.87 | 0.94 | -2.53 |
Nuclear Periphery | -1.32 | -4.54 | -2.46 | -2.32 | -1.92 | -0.63 | -2.28 | -2.63 | -1.55 | -0.52 | -1.44 | -4.56 | -2.86 | -2.52 | -2.01 |
Nuclear Periphery Foci | -1.93 | -3.25 | -3.61 | -2.34 | -0.60 | 0.81 | -3.29 | -2.29 | -2.55 | 1.38 | -1.98 | -4.27 | -3.96 | -2.11 | 0.46 |
Nucleolus | -0.67 | 2.97 | -2.26 | -1.34 | -3.40 | 0.88 | 1.06 | 0.66 | -0.86 | -2.00 | -0.22 | 2.29 | -0.79 | -0.46 | -3.80 |
Nucleus | 1.93 | 0.33 | -4.41 | -4.80 | -4.41 | -0.32 | 0.81 | 0.79 | 0.94 | -0.24 | -0.02 | 1.00 | -1.36 | -1.17 | -4.36 |
Peroxisomes | 7.99 | -2.47 | 3.66 | -4.83 | 5.16 | -3.47 | -6.49 | -4.68 | -0.76 | 2.44 | 4.25 | -4.56 | 0.36 | -4.41 | 5.19 |
Vacuole | -3.67 | -8.45 | -17.76 | -17.01 | -10.71 | -3.84 | -11.88 | -15.70 | -11.35 | -2.75 | -5.60 | -14.23 | -22.28 | -19.81 | -10.22 |
Vacuole Periphery | -2.24 | 6.55 | 0.17 | 2.16 | -5.20 | 0.70 | 7.29 | 2.58 | 1.69 | -5.98 | -0.86 | 9.30 | 2.00 | 2.95 | -7.80 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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