Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0.05
Bud 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.12 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.25 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 – – 0.06 0.05 0 0.06 0 0 0.05
Golgi 0 0.08 0 0 0 0 – 0 0 0 0 0 0.05 0.07 0.09 – – 0.12 0.06 0.08 0 0.07 0.1 0.07
Mitochondria 0.88 0.87 0.97 0.97 0.9 0.91 – 0.92 0.95 0.93 0.94 0.95 0.88 0.89 0.84 – – 0.06 0.76 0.73 0.77 0.7 0.64 0.66
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
Nuclear Periphery 0.06 0.05 0.05 0.07 0.06 0.1 – 0.07 0.12 0.11 0.11 0.12 0 0.05 0 – – 0.19 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0.11 – 0.08 0.09 0.1 0.11 0.08 0.05 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.05 0.06 – – 0 0 0 0.08 0.07 0.06 0.06
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.1 0.05 0 0.07 0 – 0 0.05 0 0 0 0.11 0.08 0.14 – – 0.12 0.05 0 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 1 1 0 – 0 0 1 0 1 0 0 0 – – 0 5 6 1 3 6 18
Bud 3 0 14 5 8 8 – 20 15 13 15 22 6 3 2 – – 0 1 0 0 1 2 4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – 0 0 0 1 1 1 2
Bud Site 0 0 4 0 1 2 – 6 8 2 2 12 0 0 0 – – 0 – – – – – –
Cell Periphery 7 5 11 9 1 4 – 3 2 7 2 5 8 12 7 – – 2 0 0 0 0 1 2
Cytoplasm 1 5 4 7 14 13 – 13 8 8 5 8 7 13 11 – – 4 0 0 0 0 4 1
Endoplasmic Reticulum 8 2 1 3 2 5 – 6 1 9 4 5 2 4 4 – – 1 0 0 0 1 1 2
Endosome 8 2 2 0 12 4 – 11 0 1 1 0 13 17 22 – – 1 24 5 19 5 15 20
Golgi 12 13 1 0 1 0 – 1 1 1 0 1 18 31 41 – – 2 27 12 11 12 37 28
Mitochondria 388 139 282 324 377 426 – 752 562 691 495 629 317 376 396 – – 1 346 122 231 130 229 267
Nucleus 4 0 0 0 3 5 – 9 4 1 2 1 1 0 0 – – 1 2 1 1 1 0 1
Nuclear Periphery 26 8 16 22 23 49 – 57 69 83 58 80 15 20 14 – – 3 5 2 2 1 5 4
Nucleolus 13 4 13 8 20 50 – 69 54 77 58 53 19 11 7 – – 0 1 1 0 1 4 2
Peroxisomes 5 2 5 4 13 6 – 23 4 4 0 6 17 22 29 – – 0 14 4 24 13 21 23
SpindlePole 2 0 0 1 5 7 – 17 8 9 4 6 5 3 2 – – 0 0 2 1 1 1 2
Vac/Vac Membrane 103 16 16 11 29 17 – 31 29 22 17 11 39 32 66 – – 2 22 5 1 7 17 21
Unique Cell Count 439 159 291 335 418 467 820 594 745 526 664 362 421 472 16 455 169 301 187 357 407
Labelled Cell Count 581 197 370 395 510 596 1018 765 929 663 840 467 544 601 17 455 169 301 187 357 407


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 9.0 5.3 6.6 7.1 6.3 – 6.4 5.9 6.4 6.5 6.3 8.4 8.5 8.2 – – 8.4 8.4 8.9 8.6
Std Deviation (1e-4) 1.3 1.4 1.1 1.5 1.6 1.6 – 1.7 1.7 1.6 1.9 1.8 1.6 1.5 1.7 – – 2.2 2.0 2.4 2.2
Intensity Change (Log2) – – – 0.31 0.42 0.25 – 0.27 0.16 0.28 0.29 0.26 0.67 0.68 0.63 – – 0.67 0.66 0.75 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Bud -2.4 -2.2 -2.5 – -2.0 -1.8 -2.8 -1.4 -1.1 -2.3 -3.5 -4.1 – – 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Cell Periphery -0.8 0 -2.8 – 0 0 -3.1 0 0 -1.2 -0.7 -2.0 – – 0
Cytoplasm 0.7 1.6 1.3 – 0 0 0 0 0 0 1.5 0.9 – – 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Endosome 0 2.1 0 – 0 0 0 0 0 2.5 2.7 3.1 – – 0
Golgi 0 0 0 – 0 0 0 0 0 3.5 4.4 4.9 – – 0
Mitochondria -0.1 -3.4 -3.1 – -3.0 -1.5 -2.5 -1.8 -1.5 -4.3 -3.8 -5.5 – – -13.2
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Nuclear Periphery 0.6 0 2.4 – 0.9 2.9 2.8 2.6 3.1 -0.8 -0.4 -1.7 – – 0
Nucleolus -1.4 0.2 3.0 – 2.2 2.4 3.0 3.2 2.0 0.5 -1.3 -2.5 – – 0
Peroxisomes 0 1.2 0 – 1.0 0 0 0 0 2.1 2.4 2.9 – – 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 – – 0
Vacuole -1.4 0.8 -1.2 – -1.3 -0.4 -2.0 -1.6 -3.3 2.4 1.1 3.7 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.8082 4.2159 3.7341 3.4108 3.4168 4.1675 4.482 4.355 4.2508 4.1103 4.6105 4.5504 5.4649 5.696 5.5042 5.332 5.5765 5.6421
Actin 0.0047 0.002 0.0106 0.0189 0.0653 0.001 0.0059 0.0031 0.0104 0.0014 0.009 0.0012 0.0109 0.0014 0.0023 0.0013 0.0007 0.0006
Bud 0.0002 0.0002 0.0002 0.0003 0.0009 0.0001 0.0005 0.002 0.0005 0.0008 0.0004 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0
Bud Neck 0.0002 0.0001 0.0003 0.0005 0.0005 0.0001 0.0046 0.0003 0.0005 0 0.0002 0.0001 0.0003 0.0002 0.0001 0 0 0.0001
Bud Periphery 0.0005 0.0003 0.0004 0.0004 0.0019 0.0002 0.001 0.0013 0.0011 0.0065 0.0004 0.0002 0.0005 0.0005 0.0002 0.0004 0.0001 0.0001
Bud Site 0.0005 0.0002 0.0008 0.001 0.0017 0.0001 0.0062 0.0091 0.01 0.0002 0.0008 0.0001 0.001 0.0006 0.0002 0.0001 0.0001 0.0001
Cell Periphery 0.0002 0.0001 0.0002 0.0001 0.0003 0.0001 0.0004 0.0013 0.0005 0.0005 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0 0.0001
Cytoplasm 0.0007 0.0002 0.0006 0.0007 0.0027 0.0001 0.0019 0.0005 0.0003 0 0.0001 0.0002 0.0004 0.0002 0.0001 0 0.0001 0
Cytoplasmic Foci 0.0178 0.0065 0.0111 0.0143 0.0194 0.0023 0.0227 0.0166 0.0042 0.0009 0.0024 0.004 0.0107 0.0113 0.0036 0.0025 0.0044 0.0009
Eisosomes 0.0002 0.0001 0.0003 0.0003 0.0005 0.0001 0.0003 0.0002 0.0004 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0001
Endoplasmic Reticulum 0.0006 0.0002 0.0006 0.0007 0.0038 0.0002 0.001 0.0003 0.0002 0.0002 0 0.0006 0.001 0.0002 0.0001 0 0 0
Endosome 0.0207 0.032 0.0201 0.015 0.0326 0.0081 0.0423 0.0244 0.0128 0.0014 0.0076 0.0137 0.0231 0.0303 0.0108 0.0016 0.0152 0.0046
Golgi 0.0217 0.0588 0.0223 0.0144 0.0271 0.0081 0.02 0.0315 0.013 0.0029 0.0063 0.0114 0.0211 0.0418 0.017 0.006 0.015 0.0129
Lipid Particles 0.0458 0.0117 0.0116 0.025 0.0241 0.006 0.0273 0.0108 0.0097 0.0004 0.0017 0.0102 0.0359 0.0144 0.0184 0.0005 0.0019 0.0068
Mitochondria 0.8411 0.854 0.8883 0.8842 0.7694 0.9618 0.8069 0.8609 0.9101 0.9469 0.9575 0.9337 0.8371 0.853 0.9239 0.9747 0.9514 0.9637
None 0.0018 0.0001 0.0026 0.0032 0.0014 0.0001 0.0008 0.0001 0.0002 0.0001 0 0.0001 0.0006 0.0001 0 0 0 0
Nuclear Periphery 0.0012 0.0002 0.0016 0.0009 0.0023 0.0002 0.0089 0.0005 0.0002 0.0001 0 0.0084 0.0122 0.0002 0.0001 0 0 0
Nucleolus 0.0005 0.0001 0.0006 0.0002 0.0003 0.0001 0.0012 0.0015 0.0007 0 0.0001 0.0001 0.0005 0.0005 0.0001 0 0 0.0001
Nucleus 0.0003 0.0001 0.0007 0.0002 0.0006 0.0001 0.0013 0.0006 0.0002 0.0001 0 0.0007 0.003 0.0002 0 0 0 0
Peroxisomes 0.0291 0.019 0.0162 0.0145 0.0324 0.0021 0.0271 0.0247 0.0181 0.0349 0.0112 0.0059 0.0245 0.0317 0.016 0.0118 0.0096 0.0078
Punctate Nuclear 0.0033 0 0.0036 0.0025 0.0007 0.005 0.0068 0.0001 0.0001 0 0 0.0003 0.001 0.0001 0 0 0 0
Vacuole 0.0036 0.0043 0.0035 0.0008 0.0033 0.001 0.0052 0.0058 0.0034 0.0007 0.0008 0.0018 0.0065 0.0062 0.0021 0.0001 0.0005 0.0004
Vacuole Periphery 0.0052 0.0097 0.004 0.0018 0.0089 0.0031 0.0081 0.0043 0.0034 0.0017 0.0013 0.0073 0.0092 0.0063 0.0046 0.0005 0.0008 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.3469 23.3186 34.6068 43.5896 27.0565 18.2495 45.3865 52.5134 66.8676 43.2688
Translational Efficiency 4.3635 2.7164 1.5827 1.7092 2.315 4.1309 1.9462 1.6165 1.4649 1.8269

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1806 1781 1187 1248 318 2106 3152 2218 2124 3887 4339 3466

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 810.26 965.64 1168.68 995.44 860.78 947.47 1037.70 958.86 817.82 955.80 1073.53 972.03
Standard Deviation 141.68 147.71 171.68 151.64 188.25 139.96 144.77 129.33 150.66 143.85 163.39 138.89
Intensity Change Log 2 — 0.253101 0.528423 0.296949 — 0.138436 0.269673 0.155676 — 0.195174 0.400935 0.225906

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000564 0.002910 0.001450 0.001166 0.007765 0.001097 0.000491 0.000717 0.001642 0.001928 0.000753 0.000879
Bud Neck 0.003426 0.000792 0.000962 0.009196 0.000666 0.001327 0.002304 0.005660 0.003012 0.001082 0.001937 0.006933
Bud Site 0.006655 0.003351 0.001284 0.009740 0.003564 0.002409 0.004926 0.011812 0.006192 0.002841 0.003929 0.011066
Cell Periphery 0.000613 0.000168 0.000113 0.000762 0.000993 0.000158 0.000164 0.000507 0.000670 0.000163 0.000150 0.000599
Cytoplasm 0.002140 0.000611 0.000380 0.000908 0.000449 0.000448 0.000718 0.001698 0.001887 0.000523 0.000625 0.001414
Cytoplasmic Foci 0.014987 0.028857 0.031039 0.014394 0.014968 0.017133 0.009445 0.011313 0.014984 0.022505 0.015352 0.012422
Eisosomes 0.000044 0.000059 0.000053 0.000062 0.000189 0.000043 0.000029 0.000063 0.000066 0.000050 0.000036 0.000063
Endoplasmic Reticulum 0.000390 0.000264 0.000431 0.000510 0.002949 0.000196 0.000161 0.000235 0.000773 0.000227 0.000235 0.000334
Endosome 0.039835 0.036254 0.018460 0.052404 0.012892 0.041939 0.034871 0.045422 0.035801 0.039334 0.030381 0.047936
Golgi 0.024683 0.045644 0.069783 0.021236 0.024328 0.025433 0.013888 0.013112 0.024630 0.034694 0.029179 0.016037
Lipid Particles 0.007730 0.004877 0.003752 0.005569 0.003183 0.005124 0.001331 0.001564 0.007049 0.005011 0.001993 0.003006
Mitochondria 0.853787* 0.839355* 0.816047* 0.802101* 0.898192* 0.869159* 0.867998* 0.834980* 0.860435* 0.855503* 0.853786* 0.823142*
Mitotic Spindle 0.002300 0.002988 0.009380 0.016407 0.003731 0.003556 0.017718 0.014097 0.002514 0.003296 0.015437 0.014929
None 0.000877 0.000188 0.000754 0.000463 0.000344 0.000244 0.000365 0.000412 0.000797 0.000218 0.000471 0.000430
Nuclear Periphery 0.000470 0.000165 0.001959 0.001099 0.000158 0.000299 0.000634 0.000603 0.000424 0.000237 0.000997 0.000781
Nuclear Periphery Foci 0.000409 0.000400 0.001102 0.001210 0.000193 0.000309 0.000475 0.000701 0.000376 0.000351 0.000646 0.000884
Nucleolus 0.000782 0.000411 0.000153 0.001544 0.000165 0.000274 0.000529 0.000656 0.000690 0.000337 0.000426 0.000976
Nucleus 0.002001 0.000269 0.000099 0.001049 0.003066 0.000411 0.000343 0.000383 0.002161 0.000346 0.000277 0.000623
Peroxisomes 0.016385 0.020291 0.008411 0.016583 0.014595 0.013377 0.008213 0.021912 0.016117 0.016545 0.008267 0.019993
Vacuole 0.009520 0.004569 0.010616 0.026336 0.001847 0.008456 0.018368 0.020567 0.008372 0.006675 0.016247 0.022644
Vacuole Periphery 0.012403 0.007576 0.023772 0.017261 0.005765 0.008608 0.017030 0.013586 0.011409 0.008135 0.018875 0.014909

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.58 -4.75 -1.25 2.76 0.56 1.98 2.16 2.10 1.22 -0.87 -0.51 1.65 1.41 3.38 -0.49
Bud Neck 3.15 2.95 -3.35 -5.54 -5.42 -1.65 -4.49 -8.37 -6.94 -5.56 2.66 1.47 -4.11 -8.73 -7.39
Bud Site 2.53 4.85 -1.82 -4.23 -6.29 0.41 -0.50 -2.74 -7.71 -5.44 3.09 2.07 -3.68 -8.54 -7.44
Cell Periphery 4.42 5.04 -0.66 -2.95 -3.24 1.44 1.43 0.83 -3.18 -3.12 4.18 4.29 0.46 -4.32 -4.45
Cytoplasm 2.86 3.33 2.25 -1.59 -3.20 0.01 -1.27 -4.23 -5.48 -4.14 3.02 2.78 1.02 -5.51 -4.76
Cytoplasmic Foci -6.55 -6.38 0.43 6.29 6.25 -0.67 1.80 1.17 4.07 -1.73 -4.97 -0.12 2.10 7.91 2.66
Eisosomes -3.99 -2.50 -4.01 -0.70 -1.99 1.73 1.89 1.49 -3.89 -7.10 1.22 2.36 0.25 -3.33 -7.84
Endoplasmic Reticulum 2.02 -0.62 -1.82 -4.14 -1.27 1.24 1.26 1.22 -1.43 -3.82 1.63 1.61 1.31 -3.71 -4.09
Endosome 1.18 7.90 -3.15 -4.19 -9.39 -9.25 -8.06 -10.27 -1.06 -3.63 -1.42 2.27 -4.43 -3.50 -7.55
Golgi -6.89 -9.78 1.29 7.52 10.22 -0.24 2.35 2.53 6.72 0.61 -4.67 -2.15 4.64 10.80 7.83
Lipid Particles 2.18 3.22 1.66 -0.63 -1.85 -1.41 1.62 1.39 4.64 -0.74 1.93 5.35 4.13 3.28 -2.62
Mitochondria 1.55 3.63 5.01 3.60 1.22 2.08 2.23 4.45 4.31 4.47 0.63 0.92 4.99 5.12 4.95
Mitotic Spindle -1.18 -4.43 -6.64 -6.33 -2.71 0.09 -6.03 -4.53 -7.06 1.87 -1.31 -10.80 -10.06 -9.62 0.33
None 2.67 0.59 1.66 -10.96 6.10 1.64 -0.37 -1.19 -5.39 -2.59 2.64 1.55 1.73 -10.54 2.21
Nuclear Periphery 1.22 -2.31 -1.88 -3.95 1.34 -2.08 -4.53 -2.12 -1.45 0.14 0.88 -2.11 -1.39 -3.42 0.91
Nuclear Periphery Foci 0.05 -2.62 -3.33 -2.81 -0.35 -1.55 -3.34 -2.12 -1.65 -0.94 0.25 -2.51 -2.77 -2.81 -1.28
Nucleolus 1.14 2.67 -1.48 -2.25 -3.19 -0.87 -1.83 -2.60 -2.09 -0.54 1.57 1.19 -0.94 -2.83 -2.42
Nucleus 2.34 2.61 1.27 -2.68 -3.75 0.90 0.92 0.91 0.14 -0.28 2.37 2.47 2.03 -1.80 -2.62
Peroxisomes -1.41 3.93 0.04 1.40 -3.70 0.29 1.60 -1.73 -4.08 -7.55 -0.10 4.83 -1.84 -2.05 -8.37
Vacuole 6.07 -0.79 -8.66 -11.85 -7.25 -7.95 -16.21 -13.96 -8.69 -1.43 2.29 -8.29 -11.90 -14.59 -5.09
Vacuole Periphery 4.19 -5.33 -3.23 -7.12 2.89 -1.92 -7.06 -4.78 -4.53 2.75 3.50 -6.48 -3.19 -7.93 3.64
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit
Localization
Cell Percentages mitochondrion (97%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl51

Mrpl51


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl51-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available