Standard name | |
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Human Ortholog | |
Description | Putative regulator of the Fps1p glycerol channel; multiply phosphorylated by Hog1p under osmotic stress; contains a pleckstrin homology domain; forms homodimers and heterodimerizes with paralog Ask10p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 4 | 7 | 11 | 12 | 7 | 7 | 9 | 6 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 10 | 3 | 4 | 13 |
Bud | 0 | 1 | 6 | 8 | 10 | 18 | 19 | 30 | 26 | 35 | 24 | 26 | 2 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
Bud Neck | 0 | 0 | 2 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 5 | 9 | 2 | 4 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 7 | 11 | 45 | 65 | 70 | 76 | 55 | 48 | 83 | 55 | 57 | 17 | 20 | 34 | 16 | 18 | 35 | 3 | 3 | 25 | 5 | 11 | 25 |
Cytoplasm | 165 | 190 | 343 | 348 | 419 | 386 | 477 | 568 | 298 | 394 | 234 | 262 | 140 | 232 | 248 | 111 | 131 | 172 | 156 | 172 | 243 | 74 | 132 | 184 |
Endoplasmic Reticulum | 2 | 2 | 2 | 2 | 0 | 2 | 4 | 2 | 0 | 8 | 2 | 4 | 2 | 0 | 0 | 1 | 9 | 5 | 1 | 1 | 5 | 1 | 0 | 6 |
Endosome | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 2 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 7 | 9 | 33 | 40 | 100 | 149 | 230 | 210 | 283 | 173 | 210 | 1 | 0 | 6 | 2 | 1 | 4 | 3 | 9 | 33 | 2 | 3 | 8 |
Nucleus | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 7 | 3 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 6 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 5 | 1 | 2 | 1 | 2 | 0 | 0 | 1 | 3 | 6 | 6 | 0 | 1 | 2 | 1 | 4 | 0 |
Unique Cell Count | 170 | 196 | 353 | 363 | 452 | 461 | 617 | 751 | 455 | 598 | 365 | 415 | 143 | 233 | 253 | 118 | 143 | 182 | 174 | 203 | 343 | 93 | 170 | 254 |
Labelled Cell Count | 177 | 207 | 375 | 443 | 548 | 588 | 742 | 915 | 598 | 819 | 500 | 569 | 162 | 255 | 297 | 135 | 170 | 226 | 174 | 203 | 343 | 93 | 170 | 254 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 4.2 | 3.9 | 4.0 | 4.6 | 4.3 | 4.2 | 4.2 | 3.9 | 3.9 | 4.0 | 3.7 | 5.1 | 5.1 | 4.8 | 6.9 | 7.6 | 7.5 | 5.4 | 5.7 | 6.6 |
Std Deviation (1e-4) | 0.7 | 0.6 | 0.7 | 1.5 | 1.7 | 1.2 | 1.1 | 1.2 | 1.2 | 1.0 | 1.2 | 0.9 | 0.9 | 0.7 | 0.9 | 1.3 | 2.3 | 1.9 | 1.3 | 1.3 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.24 | 0.16 | 0.11 | 0.12 | 0.01 | 0.02 | 0.05 | -0.06 | 0.39 | 0.41 | 0.29 | 0.83 | 0.97 | 0.94 | 0.48 | 0.55 | 0.76 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4977 | 1.2639 | 1.2153 | 0.7469 | 0.885 | 1.0346 | 1.4094 | 1.5869 | 1.3088 | 0.5209 | 1.0591 | 1.5007 | 1.6438 | 3.1333 | 2.2853 | 2.3694 | 2.5115 | 2.4385 |
Actin | 0.0638 | 0.0021 | 0.0054 | 0.0011 | 0.061 | 0.0051 | 0.0646 | 0.0012 | 0.0242 | 0.0043 | 0.0127 | 0.023 | 0.0008 | 0.0004 | 0.0101 | 0.0201 | 0.0208 | 0.002 |
Bud | 0.0007 | 0.0002 | 0.0043 | 0.0003 | 0.0007 | 0.0006 | 0.0083 | 0.0078 | 0.0098 | 0.0106 | 0.0065 | 0.0051 | 0.0001 | 0.0002 | 0.0009 | 0.0081 | 0.0015 | 0.0002 |
Bud Neck | 0.0018 | 0.0004 | 0.0003 | 0.0002 | 0.0009 | 0.0069 | 0.0018 | 0.0003 | 0.0117 | 0.0001 | 0.0011 | 0.0019 | 0.0001 | 0.0001 | 0.0003 | 0.0093 | 0.0028 | 0.0006 |
Bud Periphery | 0.001 | 0.0003 | 0.0017 | 0.0003 | 0.0012 | 0.0006 | 0.0145 | 0.0069 | 0.008 | 0.0547 | 0.0178 | 0.007 | 0.0001 | 0.0004 | 0.0019 | 0.0197 | 0.0044 | 0.0014 |
Bud Site | 0.0036 | 0.0098 | 0.0076 | 0.0002 | 0.0018 | 0.0003 | 0.0104 | 0.0239 | 0.0302 | 0.0005 | 0.0029 | 0.0019 | 0.0004 | 0.0017 | 0.0022 | 0.0044 | 0.0007 | 0 |
Cell Periphery | 0.0017 | 0.0013 | 0.0007 | 0.0003 | 0.0003 | 0.0003 | 0.0007 | 0.0011 | 0.0014 | 0.0001 | 0.0005 | 0.0004 | 0.0006 | 0.0015 | 0.0007 | 0.0007 | 0.0004 | 0.0004 |
Cytoplasm | 0.3426 | 0.5407 | 0.572 | 0.5916 | 0.4589 | 0.6182 | 0.244 | 0.4208 | 0.3951 | 0.4696 | 0.2496 | 0.5509 | 0.3378 | 0.3609 | 0.4258 | 0.3953 | 0.4079 | 0.4973 |
Cytoplasmic Foci | 0.0465 | 0.0043 | 0.0174 | 0.0083 | 0.0107 | 0.017 | 0.0462 | 0.0036 | 0.021 | 0.0036 | 0.0112 | 0.0164 | 0.0036 | 0.0022 | 0.0061 | 0.0348 | 0.0373 | 0.0036 |
Eisosomes | 0.0004 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.0001 | 0.0007 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0003 | 0.0002 |
Endoplasmic Reticulum | 0.008 | 0.0034 | 0.0042 | 0.0072 | 0.0029 | 0.0035 | 0.009 | 0.0019 | 0.0036 | 0.001 | 0.0072 | 0.0019 | 0.003 | 0.001 | 0.0028 | 0.0042 | 0.0063 | 0.0014 |
Endosome | 0.0431 | 0.0013 | 0.006 | 0.0119 | 0.0084 | 0.0027 | 0.042 | 0.0007 | 0.0128 | 0.0012 | 0.0569 | 0.0075 | 0.0005 | 0.0003 | 0.0023 | 0.0179 | 0.0168 | 0.0009 |
Golgi | 0.0094 | 0.0002 | 0.0026 | 0.0008 | 0.0079 | 0.0011 | 0.0144 | 0.0002 | 0.0033 | 0.0003 | 0.0077 | 0.0068 | 0.0002 | 0.0001 | 0.0008 | 0.0107 | 0.0138 | 0.0001 |
Lipid Particles | 0.0137 | 0.0006 | 0.0028 | 0.0011 | 0.0139 | 0.0005 | 0.0169 | 0.0003 | 0.0055 | 0.0001 | 0.0136 | 0.0075 | 0.0004 | 0.0001 | 0.0006 | 0.0113 | 0.0093 | 0.0001 |
Mitochondria | 0.0039 | 0.0001 | 0.0047 | 0.0005 | 0.0041 | 0.0008 | 0.0226 | 0.0004 | 0.0105 | 0.0033 | 0.0033 | 0.0022 | 0.0001 | 0.0001 | 0.0026 | 0.0155 | 0.0093 | 0.0002 |
None | 0.3901 | 0.432 | 0.3617 | 0.3697 | 0.4014 | 0.3373 | 0.4291 | 0.5274 | 0.448 | 0.4475 | 0.5877 | 0.3583 | 0.6507 | 0.6294 | 0.5357 | 0.4162 | 0.4498 | 0.4893 |
Nuclear Periphery | 0.0157 | 0.0004 | 0.0018 | 0.0018 | 0.0038 | 0.0018 | 0.0266 | 0.0003 | 0.0012 | 0.0004 | 0.0021 | 0.0009 | 0.0002 | 0.0001 | 0.0005 | 0.0083 | 0.0058 | 0.0002 |
Nucleolus | 0.0012 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0014 | 0.0002 | 0.0007 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0.0001 |
Nucleus | 0.0042 | 0.0006 | 0.0009 | 0.0008 | 0.0009 | 0.0006 | 0.0066 | 0.0009 | 0.0017 | 0.0005 | 0.001 | 0.0009 | 0.0003 | 0.0002 | 0.0006 | 0.0023 | 0.0007 | 0.0003 |
Peroxisomes | 0.0078 | 0.0001 | 0.0025 | 0.0001 | 0.0085 | 0.0009 | 0.0229 | 0.0002 | 0.004 | 0.0007 | 0.0041 | 0.005 | 0.0001 | 0.0001 | 0.0041 | 0.01 | 0.0072 | 0.0001 |
Punctate Nuclear | 0.021 | 0.0003 | 0.0015 | 0.0006 | 0.0106 | 0.0008 | 0.0108 | 0.0006 | 0.0023 | 0.0002 | 0.0009 | 0.001 | 0.0002 | 0.0001 | 0.0004 | 0.006 | 0.0012 | 0.0007 |
Vacuole | 0.0174 | 0.0014 | 0.0011 | 0.0028 | 0.0007 | 0.0007 | 0.0033 | 0.0012 | 0.0039 | 0.0011 | 0.0101 | 0.001 | 0.0005 | 0.0009 | 0.0009 | 0.0025 | 0.0018 | 0.0007 |
Vacuole Periphery | 0.0024 | 0.0001 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0031 | 0.0001 | 0.0007 | 0.0002 | 0.0021 | 0.0002 | 0 | 0 | 0.0001 | 0.0012 | 0.0017 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.965 | 17.8955 | 17.7807 | 24.8895 | 18.2566 | 21.3641 | 21.0843 | 23.9072 | 24.5265 | 25.3627 |
Translational Efficiency | 0.2281 | 0.2136 | 0.2648 | 0.2247 | 0.2644 | 0.2268 | 0.2168 | 0.2019 | 0.3098 | 0.2534 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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