Standard name
Human Ortholog
Description Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport; localizes to the matrix side of the inner mitochondrial membrane
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 – 0.06 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.32 0.15 – 0.12 0.47 0.27 0.27 0.31 0.18 0.19 0.13 0.11 0.39 0.6 0.7 – 0.49 0.53 0.11 0.05 0 0 0.09 0.09
Endoplasmic Reticulum 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0.11 0 – 0.07 0 0 0 0 0 0 0
Endosome 0 0 – 0 0.1 0.22 0.14 0.1 0 0 0 0 0.26 0.07 0.13 – 0.1 0.12 0.06 0.09 0 0.09 0.07 0.07
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Mitochondria 0.55 0.72 – 0.81 0.19 0.42 0.49 0.46 0.8 0.78 0.87 0.85 0.28 0.1 0 – 0.15 0.09 0.62 0.68 0.82 0.68 0.66 0.58
Nucleus 0 0 – 0.06 0 0 0 0 0.08 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0.16 0.13 0.1 0 0 0 0 0 0.14 0 0 – 0.11 0 0 0 0 0.05 0 0
SpindlePole 0.06 0 – 0 0.08 0 0.16 0.14 0 0.06 0.07 0.11 0.06 0.07 0.05 – 0.12 0.06 0.06 0 0 0 0 0
Vac/Vac Membrane 0.09 0.19 – 0 0.13 0.07 0.11 0.11 0 0.05 0 0 0.07 0 0 – 0.06 0.19 0.05 0.05 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 – 0 0 0 0 0 0 0 0 0 1 2 1 – 0 0 1 2 0 4 6 5
Bud 1 1 – 1 0 3 4 3 4 0 3 7 2 0 1 – 1 1 1 1 0 1 3 5
Bud Neck 1 0 – 0 0 2 0 1 0 1 0 1 0 1 1 – 1 0 1 0 0 0 1 2
Bud Site 0 0 – 0 0 1 0 1 0 0 1 4 0 0 0 – 0 1 – – – – – –
Cell Periphery 0 2 – 0 0 0 2 0 1 0 0 2 1 2 2 – 2 0 0 0 0 0 0 0
Cytoplasm 17 13 – 2 29 36 43 55 18 18 10 19 27 75 151 – 39 17 13 6 0 5 19 25
Endoplasmic Reticulum 2 4 – 0 0 0 0 1 0 1 0 0 0 14 7 – 6 1 1 0 0 0 1 3
Endosome 1 3 – 0 6 30 22 18 2 3 0 3 18 9 29 – 8 4 7 10 1 10 16 19
Golgi 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 – 3 2 0 1 0 2 1 4
Mitochondria 29 62 – 13 12 56 79 81 82 74 66 152 19 13 7 – 12 3 78 76 32 84 146 163
Nucleus 0 0 – 1 0 6 5 6 8 1 2 3 0 1 2 – 0 1 0 0 0 0 1 2
Nuclear Periphery 0 1 – 0 0 0 0 0 1 1 0 1 0 0 0 – 0 0 0 0 0 0 1 1
Nucleolus 0 0 – 0 0 0 1 2 1 0 1 4 0 0 0 – 0 0 0 0 0 0 1 1
Peroxisomes 0 2 – 0 10 17 16 7 0 2 0 6 10 5 8 – 9 1 2 3 1 6 3 8
SpindlePole 3 0 – 0 5 6 25 24 2 6 5 19 4 9 11 – 10 2 7 1 0 2 5 11
Vac/Vac Membrane 5 16 – 0 8 9 18 19 4 5 0 8 5 5 6 – 5 6 6 5 0 3 8 17
Unique Cell Count 53 86 16 62 134 160 177 102 95 76 178 69 124 216 80 32 128 113 39 124 223 280
Labelled Cell Count 59 105 17 71 166 215 218 123 112 88 229 87 136 226 96 39 128 113 39 124 223 280


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.1 – 4.7 7.2 5.5 5.5 5.9 4.8 4.7 4.1 4.4 5.2 5.2 5.0 – 5.3 5.6 5.9 5.8 6.0
Std Deviation (1e-4) 0.6 1.0 – 1.0 4.2 2.4 2.6 2.7 2.7 2.1 1.6 1.9 1.5 0.7 1.2 – 2.5 2.3 1.9 1.8 1.9
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0101 1.6843 1.1486 1.0662 0.709 1.3134 2.4574 2.4433 2.389 2.4559 3.0494 2.3683 -0.5265 -0.3461 -0.5946 -0.6062 -0.6877 -0.6881
Actin 0.0444 0.0041 0.0167 0.0035 0.0415 0.0145 0.0564 0.0327 0.0426 0.0168 0.02 0.0117 0.0645 0.0091 0.0355 0.0875 0.0551 0.0381
Bud 0.0007 0.0013 0.0033 0.0008 0.001 0.0009 0.0012 0.0049 0.0042 0.0009 0.0006 0.0007 0.0007 0.0021 0.0039 0.002 0.0037 0.0014
Bud Neck 0.0064 0.0025 0.0114 0.0015 0.0021 0.0119 0.0032 0.0012 0.0019 0.0016 0.0017 0.0067 0.0041 0.0105 0.0082 0.0018 0.0064 0.0172
Bud Periphery 0.0018 0.0033 0.0066 0.0025 0.0024 0.004 0.0024 0.0112 0.0103 0.0022 0.0014 0.0019 0.001 0.0045 0.0064 0.0051 0.0096 0.004
Bud Site 0.009 0.0232 0.0226 0.002 0.0021 0.0019 0.0126 0.0366 0.0212 0.003 0.0011 0.001 0.0113 0.0465 0.0286 0.0026 0.0218 0.0028
Cell Periphery 0.0023 0.0032 0.0041 0.001 0.0009 0.0028 0.0018 0.0081 0.0029 0.0009 0.0007 0.0005 0.0006 0.0024 0.0014 0.0015 0.0029 0.0028
Cytoplasm 0.0224 0.0449 0.0112 0.051 0.0519 0.0309 0.0387 0.0615 0.0429 0.0144 0.1018 0.0202 0.035 0.0476 0.0571 0.0546 0.0253 0.0455
Cytoplasmic Foci 0.1093 0.1239 0.0757 0.0385 0.0794 0.1029 0.1507 0.0901 0.0987 0.0963 0.0835 0.1046 0.1423 0.0944 0.1185 0.0365 0.0769 0.0781
Eisosomes 0.004 0.003 0.0024 0.0007 0.0007 0.0011 0.0011 0.0034 0.002 0.0013 0.0004 0.0005 0.0006 0.0012 0.0008 0.001 0.0023 0.0005
Endoplasmic Reticulum 0.0052 0.0232 0.0031 0.0011 0.0081 0.002 0.0064 0.0032 0.0095 0.0012 0.0018 0.0015 0.0063 0.0042 0.0064 0.0041 0.0035 0.0043
Endosome 0.0842 0.1087 0.0571 0.0241 0.1113 0.09 0.0954 0.074 0.0934 0.0902 0.0376 0.0881 0.1838 0.1846 0.1203 0.1125 0.106 0.1601
Golgi 0.0391 0.0414 0.0484 0.0248 0.0743 0.0467 0.0697 0.0715 0.0768 0.0532 0.0549 0.0713 0.0861 0.1102 0.0558 0.0334 0.0931 0.0962
Lipid Particles 0.0931 0.025 0.0299 0.0252 0.0557 0.0371 0.0698 0.0267 0.0185 0.0056 0.095 0.0247 0.1245 0.0227 0.0401 0.0327 0.0277 0.0189
Mitochondria 0.3636 0.4004 0.5612 0.6358 0.4119 0.4501 0.3202 0.4261 0.4119 0.4098 0.4291 0.512 0.1896 0.2736 0.3814 0.565 0.4352 0.4344
None 0.0959 0.0907 0.0304 0.0831 0.044 0.1184 0.029 0.0545 0.0646 0.1954 0.0736 0.0474 0.0397 0.1173 0.0643 0.0204 0.047 0.0217
Nuclear Periphery 0.003 0.0051 0.004 0.0002 0.0049 0.0012 0.0033 0.0008 0.0008 0.0004 0.0005 0.0003 0.0072 0.0006 0.0035 0.0017 0.0007 0.0028
Nucleolus 0.0033 0.0022 0.002 0.0004 0.0006 0.0013 0.0009 0.0008 0.0006 0.0006 0.0003 0.0003 0.0009 0.0012 0.0008 0.0006 0.0006 0.0013
Nucleus 0.0024 0.002 0.0012 0.0005 0.0014 0.0017 0.0011 0.0011 0.0008 0.0006 0.0003 0.0003 0.0014 0.0021 0.0024 0.0009 0.0007 0.0034
Peroxisomes 0.0912 0.0779 0.0964 0.0985 0.0916 0.0671 0.1253 0.069 0.0797 0.09 0.0916 0.0991 0.0811 0.0496 0.0528 0.0181 0.0741 0.0456
Punctate Nuclear 0.0059 0.0012 0.0054 0.0003 0.0056 0.001 0.0036 0.0005 0.0007 0.0013 0.0013 0.0008 0.0028 0.0016 0.002 0.0007 0.0006 0.0078
Vacuole 0.0099 0.0094 0.0045 0.0026 0.0054 0.0076 0.0048 0.0174 0.0111 0.008 0.0014 0.0037 0.0103 0.0106 0.0065 0.0114 0.0043 0.0088
Vacuole Periphery 0.0031 0.0036 0.0023 0.0017 0.0031 0.0049 0.0024 0.0047 0.0048 0.0064 0.0015 0.0028 0.0062 0.0033 0.003 0.0058 0.0024 0.0042

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.5997 5.9135 7.0209 9.1524 4.2884 6.6115 5.6802 4.0355 7.1621 8.933
Translational Efficiency 0.6012 0.7609 0.691 0.566 0.7459 0.8493 0.9214 1.2699 0.8184 0.7529

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1347 939 2215 509 1419 1836 2581 1363 2766 2775 4796 1872

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 637.38 654.87 818.73 863.96 675.40 797.08 813.62 852.57 656.88 748.96 815.98 855.67
Standard Deviation 90.45 85.37 106.84 117.88 74.79 363.44 96.82 114.94 84.94 307.22 101.61 115.86
Intensity Change Log 2 — 0.039055 0.361234 0.438811 — 0.238982 0.268613 0.336076 — 0.145367 0.314325 0.386870

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000795 0.002068 0.009592 0.018573 0.001605 0.000670 0.004733 0.020446 0.001211 0.001143 0.006977 0.019937
Bud Neck 0.022154 0.031397 0.029692 0.019417 0.012325 0.030915 0.013546 0.018449 0.017112 0.031078 0.021003 0.018712
Bud Site 0.007692 0.031654 0.070503 0.088074 0.010493 0.011609 0.040649 0.093541 0.009129 0.018392 0.054437 0.092055
Cell Periphery 0.000635 0.000425 0.000213 0.000337 0.000390 0.000262 0.000201 0.000547 0.000509 0.000317 0.000206 0.000490
Cytoplasm 0.025193 0.039135 0.071587 0.030467 0.019762 0.041124 0.017370 0.039196 0.022407 0.040451 0.042410 0.036823
Cytoplasmic Foci 0.132024 0.159270 0.135971 0.150363 0.146284 0.191934* 0.085902 0.116394 0.139340 0.180881* 0.109026 0.125630
Eisosomes 0.000312 0.000204 0.000135 0.000224 0.000246 0.000212 0.000103 0.000204 0.000278 0.000210 0.000118 0.000210
Endoplasmic Reticulum 0.000856 0.001075 0.008728 0.002706 0.000826 0.000460 0.000894 0.002046 0.000841 0.000668 0.004512 0.002225
Endosome 0.064881 0.109041 0.053194 0.091717 0.068355 0.103865 0.133105 0.099649 0.066663 0.105617 0.096199 0.097492
Golgi 0.126277* 0.112307* 0.050103 0.084366 0.150608* 0.097508 0.109607 0.107852 0.138759* 0.102516 0.082125 0.101466
Lipid Particles 0.020931 0.009101 0.005596 0.003768 0.012269 0.018942 0.002158 0.005496 0.016487 0.015612 0.003746 0.005026
Mitochondria 0.490469* 0.346383* 0.176060* 0.144973* 0.479121* 0.349272* 0.385172* 0.239402* 0.484647* 0.348294* 0.288595* 0.213726*
Mitotic Spindle 0.004397 0.013833 0.155883* 0.075082 0.007480 0.011179 0.058181 0.054806 0.005979 0.012077 0.103304* 0.060319
None 0.002230 0.000764 0.000941 0.000182 0.000244 0.001144 0.000225 0.000275 0.001211 0.001015 0.000556 0.000250
Nuclear Periphery 0.000311 0.000118 0.002787 0.000545 0.000107 0.000432 0.000269 0.000457 0.000206 0.000326 0.001432 0.000481
Nuclear Periphery Foci 0.000324 0.000290 0.002006 0.002450 0.000149 0.000252 0.000425 0.001087 0.000234 0.000265 0.001155 0.001457
Nucleolus 0.002353 0.002301 0.002819 0.001073 0.000445 0.002760 0.000864 0.001125 0.001374 0.002605 0.001767 0.001111
Nucleus 0.007946 0.008695 0.046734 0.011521 0.002936 0.013277 0.003388 0.005229 0.005376 0.011727 0.023407 0.006940
Peroxisomes 0.070707 0.093619 0.062790 0.164150* 0.065422 0.075656 0.077766 0.113141* 0.067996 0.081734 0.070849 0.127011*
Vacuole 0.009272 0.026002 0.102275 0.101421 0.011564 0.037518 0.051107 0.066120 0.010448 0.033621 0.074739 0.075718
Vacuole Periphery 0.010240 0.012318 0.012390 0.008590 0.009368 0.011008 0.014336 0.014537 0.009793 0.011451 0.013437 0.012920

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.37 -10.32 -5.82 -5.37 -2.86 2.22 -4.77 -9.02 -9.66 -7.43 0.27 -10.93 -10.89 -10.99 -7.33
Bud Neck -2.54 -3.32 0.50 3.01 4.00 -8.24 -0.95 -3.26 4.97 -2.77 -6.85 -3.07 -1.17 5.83 1.70
Bud Site -9.64 -23.02* -12.10 -8.02 -2.48 -0.81 -14.53 -19.72 -19.65 -11.79 -7.84 -26.70 -23.44 -20.42 -9.97
Cell Periphery 2.35 5.97 3.95 1.41 -3.49 1.46 2.18 -1.43 -4.77 -6.02 3.21 5.39 0.42 -3.52 -6.60
Cytoplasm -3.62 -14.18 -2.62 0.97 9.88 -7.21 0.85 -7.20 -0.07 -8.37 -7.59 -11.18 -7.11 0.29 1.98
Cytoplasmic Foci -4.15 0.06 -2.08 1.44 -2.28 -7.90 13.05 5.58 13.84 -7.18 -9.55 9.29 3.46 12.49 -4.76
Eisosomes 3.46 6.19 2.63 -1.02 -5.24 1.58 8.09 2.17 0.61 -9.70 3.64 9.66 3.76 0.05 -10.24
Endoplasmic Reticulum -0.52 -12.10 -6.33 -4.10 8.69 2.28 -0.73 -5.06 -8.04 -5.41 0.98 -11.64 -7.38 -8.01 6.89
Endosome -7.40 3.75 -3.59 2.85 -6.37 -7.83 -14.61 -6.23 1.09 6.76 -10.81 -9.43 -7.46 2.39 -0.10
Golgi 2.02 15.27 6.14 4.22 -4.75 8.59 7.09 6.93 -1.47 0.73 8.00 14.67 8.32 0.98 -4.43
Lipid Particles 5.35 8.23 9.05 3.80 3.15 -3.56 7.79 4.41 8.00 -3.70 0.48 11.30 8.97 8.57 -1.91
Mitochondria 10.05 30.10 25.09 14.06 2.69 11.19 8.56 20.28 10.42 14.34* 15.31 25.24 29.94 15.64 9.51
Mitotic Spindle -4.81 -28.94* -9.84 -8.30 9.27 -2.20 -17.30 -12.43 -11.43 0.87 -4.95 -32.82 -16.18 -14.05 9.88
None 2.14 1.84 3.04 5.28 6.74 -5.71 0.11 -0.50 5.59 -0.73 0.51 1.90 2.94 6.98 5.64
Nuclear Periphery 2.70 -6.28 -1.47 -2.60 5.20 -2.42 -3.60 -3.74 -0.29 -1.82 -1.27 -6.64 -3.27 -1.51 4.75
Nuclear Periphery Foci 0.27 -8.96 -1.48 -1.50 -0.17 -2.13 -3.42 -3.14 -2.77 -2.07 -0.60 -9.36 -2.76 -2.69 -0.49
Nucleolus 0.02 -1.39 2.20 1.44 4.49 -4.60 -2.36 -2.36 2.79 -0.70 -2.75 -2.02 0.52 3.08 2.55
Nucleus -0.52 -14.83 -2.17 -1.75 9.85 -7.39 -1.12 -2.93 4.37 -2.01 -5.52 -13.87 -2.58 2.38 10.39
Peroxisomes -3.46 2.07 -7.96 -5.54 -9.08 -2.18 -2.58 -7.32 -5.88 -5.62 -3.62 -0.60 -10.39 -7.72 -10.43
Vacuole -6.88 -29.11 -12.90 -10.74 -1.29 -11.65 -17.27 -15.03 -8.06 -4.41 -13.82 -32.90 -19.82 -13.06 -1.61
Vacuole Periphery -1.62 -1.32 1.74 3.01 2.93 -1.79 -5.06 -3.63 -2.48 0.03 -2.21 -4.55 -2.66 -1.08 0.50
Standard name
Human Ortholog
Description Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport; localizes to the matrix side of the inner mitochondrial membrane
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Rrg8

Rrg8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrg8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available