Standard name
Human Ortholog
Description 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.98 0.96 0.93 0.83 0.79 0.71 0.64 0.7 0.6 0.61 0.98 0.99 0.98 0.96 0.94 0.95 0.97 0.93 0.93 0.94 0.91 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.21 0.23 0.27 0.35 0.33 0.43 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.05 0.06 0.07 0.12 0.08 0.08 0.11 0.1 0 0 0.05 0 0.09 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 0 0 0 1 1 4 4 6 2 5 2 0 1 1 0 0 0 1 2 0 3 6 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cell Periphery 0 1 2 1 2 3 4 5 2 6 10 8 3 3 2 7 10 4 0 0 0 0 0 0
Cytoplasm 167 272 268 198 310 284 368 304 189 190 184 202 525 550 599 300 345 249 165 252 255 345 466 540
Endoplasmic Reticulum 3 0 1 0 5 1 9 0 6 3 2 4 1 2 1 8 7 6 0 1 3 0 1 5
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 1 3 0 0 0 0 1 1 1 1 4 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2
Mitochondria 0 3 2 6 16 71 106 115 105 89 132 151 1 0 0 1 1 2 0 2 1 1 4 5
Nucleus 0 1 2 2 4 4 2 12 5 5 8 9 7 4 1 13 3 6 0 2 1 4 8 1
Nuclear Periphery 1 0 0 0 0 1 0 2 3 0 5 2 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 1
Vac/Vac Membrane 0 0 5 11 16 22 35 51 25 22 33 32 7 9 28 10 32 17 0 4 5 5 7 7
Unique Cell Count 170 275 274 206 334 341 467 430 296 271 305 333 534 558 611 311 366 261 171 273 275 368 514 587
Labelled Cell Count 171 277 281 218 355 387 528 493 341 318 379 411 545 572 632 339 399 284 171 273 275 368 514 587


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.7 9.4 8.8 8.0 7.5 6.8 6.3 6.2 5.7 5.7 5.3 5.0 10.9 10.6 9.8 15.6 15.2 13.7 11.1 10.9 11.0
Std Deviation (1e-4) 1.6 2.2 1.8 1.9 1.7 1.7 1.7 1.8 1.5 1.4 1.4 1.4 2.2 2.1 1.7 3.3 3.1 2.9 2.1 2.3 2.2
Intensity Change (Log2) -0.14 -0.23 -0.38 -0.49 -0.51 -0.63 -0.64 -0.75 -0.81 0.3 0.27 0.14 0.82 0.78 0.64 0.33 0.3 0.31


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 2.1 0 0 0 0 0 1.8 0
Cytoplasm -1.1 -2.8 -5.9 -7.2 -9.0 -10.2 -8.8 -10.9 -10.9 0.5 0.8 0.2 -1.0 -2.2 -1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.9 7.7 8.2 9.0 10.6 10.1 12.1 12.6 0 0 0 0 0 0
Nucleus 0 0 0 0 1.9 0 0 0 0 0 0 0 2.6 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.1 2.0 2.8 3.3 4.8 3.5 3.4 4.4 4.0 0 0 2.0 1.1 3.7 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9651 4.5356 5.0036 4.8314 5.2455 5.4429 3.7929 5.3121 4.9348 4.4069 3.7172 4.5553 3.3448 4.9163 4.465 4.9968 4.8205 4.2213
Actin 0.0755 0.0011 0.0011 0.0078 0.0653 0.0006 0.0222 0.0031 0.0237 0.0532 0.003 0.0102 0.0571 0.0014 0.0146 0.0347 0.0958 0.0167
Bud 0.001 0.0003 0.015 0.0222 0.001 0.0001 0.0009 0.0024 0.0016 0.0099 0.0005 0.0022 0.0026 0.0051 0.0178 0.0066 0.0007 0.0026
Bud Neck 0.0039 0.0003 0.0004 0.0017 0.0012 0.0008 0.0058 0.0021 0.0008 0.0011 0.0004 0.002 0.0025 0.0004 0.0006 0.0013 0.0006 0.0037
Bud Periphery 0.0014 0.0001 0.0022 0.0231 0.0023 0 0.0008 0.0006 0.0011 0.0035 0.0008 0.0028 0.0046 0.0021 0.0044 0.0035 0.0005 0.0013
Bud Site 0.0057 0.0011 0.0011 0.0265 0.0012 0.0001 0.0062 0.0105 0.0027 0.0059 0.0003 0.0024 0.0152 0.0019 0.0058 0.0046 0.0035 0.0023
Cell Periphery 0.0006 0.0001 0.0001 0.0003 0.0002 0 0.0003 0.0002 0.0001 0.0004 0 0 0.0008 0.0001 0.0003 0.0005 0.0001 0.0001
Cytoplasm 0.6384 0.7617 0.7446 0.7509 0.6209 0.8417 0.6848 0.9109 0.858 0.7587 0.7582 0.8785 0.5712 0.9156 0.8881 0.7848 0.7558 0.7934
Cytoplasmic Foci 0.0298 0.0101 0.0044 0.0115 0.0124 0.0123 0.0414 0.0091 0.0172 0.0225 0.0201 0.033 0.037 0.0055 0.0049 0.0117 0.0282 0.0132
Eisosomes 0.0006 0 0.0001 0.0002 0.0005 0 0.0002 0 0.0001 0.0002 0 0 0.0012 0 0.0001 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0.0112 0.0036 0.0036 0.0038 0.005 0.0041 0.007 0.0025 0.0017 0.0047 0.0023 0.0038 0.0312 0.0021 0.0033 0.0366 0.0062 0.0019
Endosome 0.0179 0.0083 0.0041 0.0063 0.0223 0.0108 0.034 0.0018 0.0053 0.0247 0.0206 0.0156 0.04 0.0015 0.0047 0.0204 0.0284 0.0139
Golgi 0.0073 0.0004 0.0002 0.0014 0.0076 0.0003 0.0094 0.0005 0.0018 0.0108 0.024 0.0024 0.0107 0.0003 0.0019 0.0067 0.0309 0.0061
Lipid Particles 0.0041 0.0003 0.0001 0.0038 0.0124 0.0003 0.0232 0.0003 0.0025 0.007 0.0116 0.0009 0.0081 0.0001 0.0005 0.0083 0.0083 0.0029
Mitochondria 0.0034 0.0005 0.0015 0.0025 0.0099 0.0003 0.0088 0.0003 0.0016 0.0074 0.0594 0.0015 0.0151 0.0004 0.0028 0.0022 0.0034 0.0031
None 0.1253 0.1856 0.1935 0.0811 0.1551 0.1053 0.0705 0.0413 0.063 0.0259 0.0782 0.0119 0.1189 0.0377 0.0266 0.0423 0.0091 0.087
Nuclear Periphery 0.015 0.0036 0.0033 0.0062 0.0429 0.0048 0.019 0.0017 0.0014 0.0042 0.0013 0.0051 0.0346 0.002 0.0027 0.014 0.0039 0.01
Nucleolus 0.0032 0.0003 0.0003 0.0066 0.0016 0.0001 0.0005 0.0002 0.0001 0.0009 0 0.0002 0.001 0.0001 0.0004 0.0005 0.0001 0.0017
Nucleus 0.0173 0.0154 0.0201 0.0281 0.0145 0.0116 0.031 0.0087 0.0114 0.0251 0.004 0.0183 0.0177 0.0137 0.0166 0.0067 0.0053 0.0205
Peroxisomes 0.023 0.0006 0.0002 0.004 0.0063 0.0002 0.0125 0.0006 0.0033 0.0242 0.012 0.0027 0.0169 0.0084 0.0003 0.0013 0.0136 0.0036
Punctate Nuclear 0.0134 0.0033 0.0017 0.0092 0.0124 0.0052 0.0104 0.0022 0.0018 0.0029 0.0007 0.0045 0.0044 0.0008 0.0027 0.0086 0.0031 0.0099
Vacuole 0.0014 0.0028 0.002 0.0023 0.0019 0.001 0.0092 0.0009 0.0007 0.0052 0.0019 0.0013 0.0047 0.0008 0.0008 0.003 0.0019 0.005
Vacuole Periphery 0.0005 0.0004 0.0004 0.0005 0.0032 0.0003 0.002 0.0001 0.0001 0.0014 0.0006 0.0005 0.0044 0.0001 0.0003 0.0014 0.0006 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 102.2167 109.3313 102.447 100.4545 104.7495 76.7316 89.3638 81.4178 86.4202 89.7973
Translational Efficiency 1.1291 1.1641 1.1494 1.0043 0.8953 1.4575 1.156 1.1574 1.0271 1.0911

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
77 94 1520 1558 2366 812 186 60 2443 906 1706 1618

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 956.13 1013.94 923.22 955.67 881.44 898.56 1225.70 1142.66 883.79 910.53 956.20 962.60
Standard Deviation 134.91 171.64 124.11 217.82 177.71 209.32 185.12 290.96 177.00 208.72 162.32 223.77
Intensity Change Log 2 0.084694 -0.050532 -0.000694 0.027752 0.475672 0.374462 0.057661 0.225813 0.191442

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000306 0.003028 0.000109 0.002622 0.000134 0.000486 0.000816 0.021830 0.000140 0.000750 0.000186 0.003335
Bud Neck 0.001399 0.010210 0.002613 0.003836 0.002960 0.003262 0.003614 0.003861 0.002911 0.003983 0.002722 0.003837
Bud Site 0.009603 0.005985 0.004000 0.025956 0.005216 0.014739 0.007890 0.014165 0.005354 0.013831 0.004424 0.025519
Cell Periphery 0.000167 0.000194 0.000170 0.000170 0.000100 0.000222 0.000266 0.002398 0.000102 0.000219 0.000181 0.000253
Cytoplasm 0.741519* 0.652161* 0.670743* 0.632378* 0.767691* 0.717934* 0.402831* 0.454132* 0.766866* 0.711110* 0.641533* 0.625768*
Cytoplasmic Foci 0.114330 0.097826 0.028490 0.021774 0.042822 0.085486 0.014352 0.023242 0.045076 0.086767 0.026948 0.021828
Eisosomes 0.000026 0.000056 0.000043 0.000030 0.000015 0.000041 0.000068 0.000266 0.000015 0.000042 0.000046 0.000039
Endoplasmic Reticulum 0.001869 0.002817 0.001033 0.001779 0.001502 0.001614 0.008028 0.007005 0.001514 0.001739 0.001795 0.001972
Endosome 0.003764 0.010986 0.002875 0.008147 0.002128 0.007117 0.005309 0.024136 0.002180 0.007518 0.003141 0.008740
Golgi 0.000613 0.004450 0.000166 0.004174 0.000771 0.003227 0.000214 0.016022 0.000766 0.003354 0.000171 0.004613
Lipid Particles 0.001010 0.004320 0.001075 0.001278 0.000699 0.002656 0.002715 0.001655 0.000709 0.002829 0.001253 0.001292
Mitochondria 0.000426 0.001335 0.000294 0.002967 0.000650 0.003663 0.000417 0.001388 0.000643 0.003421 0.000307 0.002909
Mitotic Spindle 0.005441 0.002227 0.000935 0.030791 0.001818 0.008804 0.002872 0.014645 0.001932 0.008122 0.001147 0.030192
None 0.001622 0.001142 0.005579 0.009517 0.008973 0.004992 0.004199 0.008906 0.008741 0.004592 0.005429 0.009495
Nuclear Periphery 0.000212 0.000466 0.000460 0.000718 0.000344 0.000248 0.001857 0.001522 0.000340 0.000270 0.000612 0.000748
Nuclear Periphery Foci 0.003095 0.004331 0.001129 0.003233 0.000831 0.002228 0.007912 0.002004 0.000902 0.002447 0.001868 0.003188
Nucleolus 0.000214 0.000429 0.000465 0.000282 0.000505 0.000862 0.000415 0.001454 0.000496 0.000817 0.000459 0.000325
Nucleus 0.056002 0.098190 0.212598* 0.118303 0.132355* 0.071311 0.361745* 0.277726* 0.129948 0.074100 0.228859* 0.124215
Peroxisomes 0.000524 0.005167 0.000472 0.001576 0.000291 0.001659 0.000333 0.000834 0.000299 0.002023 0.000457 0.001549
Vacuole 0.056658 0.093753 0.066453 0.126656 0.029504 0.067129 0.173857 0.122363 0.030360 0.069892 0.078163 0.126497
Vacuole Periphery 0.001200 0.000927 0.000298 0.003812 0.000691 0.002320 0.000290 0.000446 0.000707 0.002175 0.000297 0.003687

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.96 2.44 -1.97 0.35 -2.33 -3.54 -8.90 -1.10 -1.03 -0.97 -2.20 -2.12 -2.52 -1.59 -2.43
Bud Neck -1.01 -2.47 -4.92 0.72 -1.45 -0.59 -1.96 -1.93 -1.51 -0.01 -1.02 0.15 -1.49 0.31 -1.30
Bud Site 1.67 2.39 -2.58 -5.59 -6.61 -5.29 -1.80 -1.93 1.84 -0.48 -5.18 0.97 -6.30 -1.56 -6.51
Cell Periphery -0.10 0.17 0.84 1.01 1.41 -3.41 -7.18 -1.60 -1.32 -1.24 -3.57 -4.65 -3.23 1.33 0.50
Cytoplasm 2.73 3.15 7.66 3.24 11.09 6.09 23.97 11.58 9.68 0.12 6.99 17.57 26.86 14.09 8.58
Cytoplasmic Foci 1.26 6.00 6.78 5.95 6.93 -8.99 14.86 6.20 11.38 -0.35 -9.05 9.71 15.16 16.44 6.35
Eisosomes -1.95 -2.64 -0.80 1.75 3.16 -7.49 -9.31 -1.93 -1.44 -0.90 -7.94 -10.35 -7.61 1.57 2.83
Endoplasmic Reticulum -0.93 1.51 0.53 1.61 -3.58 -0.19 -6.93 -3.54 -3.40 0.44 -0.51 -0.76 -1.33 -0.33 -0.67
Endosome -2.38 1.32 -0.61 2.12 -1.87 -4.77 -4.58 -1.03 -0.15 -0.64 -5.38 -3.25 -3.09 2.30 -1.86
Golgi -1.23 2.88 -2.17 0.72 -3.05 -2.12 1.74 -0.85 -0.27 -0.99 -2.35 1.94 -1.82 1.06 -3.20
Lipid Particles -1.82 -0.03 -1.56 1.62 -2.18 -3.45 -7.77 -4.19 0.69 0.75 -3.90 -4.38 -6.64 2.46 -1.45
Mitochondria -0.92 0.45 -1.62 -0.44 -1.94 -1.90 2.83 -0.35 1.80 -1.35 -1.94 3.57 -1.36 1.15 -1.95
Mitotic Spindle 0.67 0.94 -3.05 -6.80 -7.96 -3.15 -1.72 -2.01 -1.42 -1.52 -3.10 0.16 -7.74 -3.80 -7.82
None 0.39 -3.99 -5.20 -5.78 -0.81 3.35 3.19 1.72 -0.54 -0.44 3.68 3.18 2.28 -1.66 -0.91
Nuclear Periphery -2.96 -5.15 -12.39 -5.77 -11.10 0.44 -15.05 -6.37 -6.45 -0.88 0.06 -6.67 -12.49 -12.58 -8.54
Nuclear Periphery Foci -0.94 3.47 0.74 1.56 -6.10 -4.05 -9.38 -2.09 -1.10 1.61 -4.66 -6.03 -7.94 -0.66 -3.75
Nucleolus -1.59 -2.46 -0.55 1.52 2.74 -1.04 2.01 -0.63 0.31 -1.20 -1.03 0.61 2.96 1.87 2.56
Nucleus -2.69 -14.03 -8.49 -2.52 10.04 13.25 -11.60 -4.76 -6.54 0.85 12.07 -15.52 -4.16 -14.45 11.25
Peroxisomes -1.04 0.38 -1.11 0.95 -1.25 -4.43 0.59 -0.57 3.82 -0.77 -3.44 -0.82 -2.14 1.99 -1.34
Vacuole -2.89 -1.27 -11.82 -6.91 -19.74 -10.13 -12.89 -6.46 -4.87 -0.08 -11.10 -15.76 -28.80 -16.86 -17.04
Vacuole Periphery 0.50 1.36 -0.65 -1.42 -2.87 -1.82 3.96 3.33 2.44 -0.33 -1.80 3.46 -1.92 0.39 -2.89
Standard name
Human Ortholog
Description 5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mri1

Mri1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mri1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available