Standard name | |
---|---|
Human Ortholog | |
Description | Mitochondrial inner membrane protein; exposed to the mitochondrial matrix; associates with mitochondrial ribosomes; NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 5 | 15 | 9 | 4 | 2 | 3 | 3 | 2 | 4 | 3 | 0 | 2 | 4 | 12 | 5 | 5 | 6 | 6 | 9 | 20 | 18 | 24 | 27 |
Bud | 0 | 6 | 9 | 8 | 5 | 15 | 21 | 25 | 11 | 22 | 9 | 13 | 1 | 1 | 2 | 1 | 2 | 1 | 0 | 8 | 5 | 9 | 9 | 6 |
Bud Neck | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 2 | 5 | 0 | 1 | 6 | 3 | 3 | 0 | 0 | 0 | 0 | 4 | 1 | – | – | – | – | – | – |
Cell Periphery | 1 | 12 | 15 | 8 | 1 | 1 | 5 | 4 | 8 | 4 | 4 | 4 | 8 | 12 | 14 | 7 | 12 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 4 | 22 | 25 | 27 | 36 | 23 | 35 | 40 | 26 | 47 | 33 | 26 | 12 | 37 | 17 | 64 | 80 | 55 | 0 | 7 | 12 | 6 | 18 | 9 |
Endoplasmic Reticulum | 1 | 2 | 2 | 2 | 2 | 0 | 4 | 1 | 6 | 4 | 1 | 2 | 1 | 1 | 5 | 6 | 7 | 9 | 0 | 0 | 0 | 0 | 3 | 0 |
Endosome | 0 | 0 | 2 | 1 | 5 | 2 | 3 | 10 | 2 | 0 | 1 | 0 | 2 | 8 | 6 | 6 | 8 | 2 | 1 | 5 | 3 | 4 | 7 | 7 |
Golgi | 1 | 16 | 16 | 15 | 7 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 140 | 307 | 328 | 28 | 21 | 28 | 9 | 42 | 16 | 81 | 76 | 71 |
Mitochondria | 168 | 414 | 424 | 352 | 351 | 538 | 775 | 651 | 600 | 727 | 539 | 507 | 319 | 338 | 410 | 8 | 15 | 17 | 143 | 352 | 386 | 360 | 382 | 428 |
Nucleus | 0 | 1 | 1 | 0 | 1 | 2 | 5 | 2 | 4 | 3 | 3 | 1 | 0 | 1 | 0 | 3 | 2 | 6 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 7 | 16 | 18 | 9 | 15 | 68 | 87 | 73 | 89 | 75 | 46 | 45 | 6 | 4 | 8 | 2 | 6 | 4 | 0 | 2 | 1 | 4 | 5 | 5 |
Nucleolus | 13 | 14 | 11 | 9 | 32 | 85 | 124 | 110 | 65 | 94 | 55 | 44 | 11 | 11 | 6 | 6 | 20 | 16 | 2 | 0 | 2 | 2 | 4 | 3 |
Peroxisomes | 1 | 9 | 41 | 18 | 11 | 9 | 5 | 33 | 19 | 8 | 3 | 9 | 3 | 7 | 8 | 2 | 14 | 4 | 5 | 9 | 45 | 11 | 9 | 31 |
SpindlePole | 0 | 1 | 3 | 0 | 2 | 2 | 9 | 19 | 9 | 9 | 3 | 4 | 1 | 3 | 1 | 3 | 6 | 1 | 0 | 5 | 4 | 3 | 4 | 4 |
Vac/Vac Membrane | 11 | 32 | 7 | 6 | 11 | 15 | 13 | 23 | 19 | 15 | 15 | 14 | 43 | 97 | 43 | 19 | 26 | 21 | 1 | 12 | 0 | 15 | 14 | 8 |
Unique Cell Count | 174 | 460 | 487 | 391 | 421 | 598 | 863 | 809 | 682 | 818 | 589 | 556 | 397 | 572 | 552 | 143 | 199 | 145 | 172 | 460 | 505 | 522 | 563 | 610 |
Labelled Cell Count | 207 | 552 | 589 | 464 | 484 | 764 | 1094 | 994 | 862 | 1021 | 718 | 675 | 549 | 831 | 860 | 160 | 229 | 173 | 172 | 460 | 505 | 522 | 563 | 610 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.8 | 14.7 | 16.2 | 14.6 | 13.6 | 12.4 | 11.6 | 11.7 | 11.6 | 10.8 | 10.2 | 10.9 | 18.7 | 18.2 | 16.8 | 17.1 | 15.4 | 16.9 | 20.2 | 18.9 | 21.6 |
Std Deviation (1e-4) | 3.6 | 4.3 | 4.3 | 4.2 | 4.0 | 3.5 | 3.4 | 3.6 | 3.4 | 4.0 | 3.6 | 3.9 | 4.3 | 5.7 | 3.7 | 9.8 | 7.6 | 8.2 | 5.8 | 5.7 | 6.0 |
Intensity Change (Log2) | – | – | – | -0.15 | -0.25 | -0.38 | -0.49 | -0.46 | -0.49 | -0.59 | -0.67 | -0.58 | 0.21 | 0.17 | 0.05 | 0.07 | -0.07 | 0.06 | 0.32 | 0.22 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.7 | -2.2 | -3.6 | -4.2 | -4.0 | -3.9 | -3.8 | -3.3 | -4.2 | -2.8 | -2.9 | -0.9 | 0 | -0.4 | 0 |
Bud | 0.2 | -0.8 | 0.7 | 0.7 | 1.4 | -0.3 | 1.0 | -0.4 | 0.5 | 0 | 0 | -2.3 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.0 | -3.2 | -4.0 | -3.7 | -3.8 | -2.3 | -3.8 | -3.0 | -2.8 | -1.0 | -1.0 | -0.5 | 0 | 1.8 | 0 |
Cytoplasm | 1.1 | 2.1 | -1.0 | -0.9 | -0.2 | -1.1 | 0.5 | 0.3 | -0.3 | -1.6 | 0.9 | -1.7 | 12.0 | 11.6 | 10.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.4 | -1.6 | -4.5 | -5.4 | -5.2 | -4.8 | -4.3 | -4.4 | -3.3 | 12.4 | 17.7 | 19.2 | 6.7 | 3.8 | 6.7 |
Mitochondria | 1.4 | -1.6 | 1.5 | 1.5 | -3.1 | 0.5 | 1.0 | 2.4 | 2.1 | -2.7 | -10.1 | -5.2 | -18.5 | -19.7 | -17.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.2 | -0.1 | 4.7 | 4.2 | 3.6 | 5.5 | 3.7 | 2.8 | 3.0 | -2.0 | -3.4 | -2.3 | 0 | -0.4 | -0.5 |
Nucleolus | 0 | 3.8 | 6.9 | 7.1 | 6.8 | 5.0 | 5.9 | 4.8 | 4.1 | 0.5 | -0.4 | -1.5 | 0 | 4.5 | 4.6 |
Peroxisomes | -2.2 | -3.8 | -5.4 | -7.6 | -3.3 | -4.3 | -6.8 | -6.5 | -5.1 | -5.2 | -5.6 | -5.3 | -2.9 | -0.6 | -2.3 |
SpindlePole | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 1.3 | 1.2 | 0.1 | 1.6 | 1.5 | 0.5 | 1.3 | 1.2 | 6.0 | 8.5 | 4.8 | 6.3 | 6.5 | 6.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 20.333 | 21.206 | 22.1182 | 19.6311 | 20.1894 | 20.5023 | 18.6336 | 22.7407 | 20.9145 | 18.2109 | 20.48 | 20.5402 | 20.4849 | 21.8847 | 21.7125 | 21.1313 | 19.9088 | 21.1026 |
Actin | 0.0167 | 0.0083 | 0.008 | 0.0011 | 0.0004 | 0.0008 | 0.0079 | 0.0032 | 0.0121 | 0.0315 | 0.0075 | 0.0007 | 0.0076 | 0.0013 | 0.0011 | 0.0035 | 0.0033 | 0.0003 |
Bud | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0.0007 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0004 | 0 | 0.0007 | 0.0005 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 |
Bud Periphery | 0.0008 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0009 | 0.0001 | 0.0004 | 0.0004 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0003 | 0.0012 | 0.0039 | 0.0001 | 0 | 0 | 0.0025 | 0.0001 | 0.0054 | 0.001 | 0.0005 | 0 | 0.0005 | 0 | 0 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0006 | 0.0007 | 0.0004 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0012 | 0 | 0.0005 | 0.0012 | 0.0055 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 |
Cytoplasmic Foci | 0.0012 | 0.0012 | 0.0011 | 0.0007 | 0.0002 | 0.0003 | 0.0088 | 0.0005 | 0.0029 | 0.0057 | 0.0045 | 0.0001 | 0.0003 | 0.0007 | 0 | 0.0001 | 0.0007 | 0 |
Eisosomes | 0.0004 | 0.0011 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0003 | 0.0006 | 0.001 | 0.0003 | 0.0003 | 0.0006 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0012 | 0.0001 | 0.0012 | 0.0017 | 0.0034 | 0.0001 | 0 | 0 | 0 | 0 | 0.001 | 0 |
Endosome | 0.0017 | 0.0092 | 0.0031 | 0.0021 | 0.0009 | 0.0024 | 0.0133 | 0.0027 | 0.0033 | 0.0056 | 0.0034 | 0.0012 | 0.0011 | 0.0026 | 0.0004 | 0.0008 | 0.005 | 0.0005 |
Golgi | 0.0059 | 0.0353 | 0.0119 | 0.0034 | 0.0173 | 0.0066 | 0.0081 | 0.0163 | 0.01 | 0.0181 | 0.005 | 0.0048 | 0.0042 | 0.0197 | 0.0046 | 0.0011 | 0.0028 | 0.013 |
Lipid Particles | 0.0191 | 0.0053 | 0.0008 | 0.0138 | 0.0005 | 0.0024 | 0.0164 | 0.0098 | 0.0046 | 0.008 | 0.0149 | 0.0071 | 0.0024 | 0.003 | 0.0027 | 0.0017 | 0.0013 | 0.0005 |
Mitochondria | 0.9022 | 0.9011 | 0.9531 | 0.9585 | 0.9785 | 0.9737 | 0.8989 | 0.9522 | 0.952 | 0.9 | 0.9399 | 0.9661 | 0.9776 | 0.9673 | 0.9875 | 0.9838 | 0.9623 | 0.982 |
None | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0015 | 0 | 0.0006 | 0.0022 | 0.003 | 0 | 0.0001 | 0 | 0 | 0 | 0.001 | 0 |
Nuclear Periphery | 0.0013 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0058 | 0 | 0.0003 | 0.004 | 0.0025 | 0.0092 | 0.0001 | 0 | 0.0001 | 0 | 0.0121 | 0 |
Nucleolus | 0.0153 | 0.001 | 0.0001 | 0.0001 | 0 | 0 | 0.002 | 0.0001 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0 |
Nucleus | 0.0037 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0.0018 | 0 | 0.0001 | 0.0011 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0 | 0.0022 | 0 |
Peroxisomes | 0.0013 | 0.0029 | 0.0009 | 0.0031 | 0.0011 | 0.0011 | 0.0076 | 0.0031 | 0.0019 | 0.0119 | 0.0015 | 0.0004 | 0.0013 | 0.0028 | 0.0006 | 0.0018 | 0.0005 | 0.0003 |
Punctate Nuclear | 0.0017 | 0 | 0 | 0 | 0 | 0 | 0.0046 | 0 | 0.0002 | 0.0032 | 0.0048 | 0.0001 | 0 | 0 | 0 | 0 | 0.0051 | 0 |
Vacuole | 0.0047 | 0.0089 | 0.0031 | 0.0014 | 0.0001 | 0.0029 | 0.0032 | 0.0014 | 0.0004 | 0.0008 | 0.0005 | 0.0004 | 0.001 | 0.0004 | 0.0002 | 0.0011 | 0.0003 | 0.0002 |
Vacuole Periphery | 0.0224 | 0.0217 | 0.0113 | 0.0153 | 0.0006 | 0.0094 | 0.0118 | 0.0097 | 0.0019 | 0.0014 | 0.0012 | 0.0093 | 0.0028 | 0.0016 | 0.0022 | 0.0057 | 0.001 | 0.003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.801 | 21.6786 | 27.347 | 31.7728 | 31.442 | 33.9321 | 24.4764 | 22.191 | 29.3449 | 31.7594 |
Translational Efficiency | 1.6136 | 2.1697 | 1.6818 | 1.5078 | 1.4106 | 1.7379 | 1.5913 | 1.512 | 1.5092 | 1.4247 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1670 | 323 | 2339 | 1291 | 2051 | 2023 | 1058 | 2419 | 3721 | 2346 | 3397 | 3710 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1285.86 | 1698.44 | 1627.00 | 1983.58 | 1390.93 | 1388.01 | 1480.90 | 2256.27 | 1343.77 | 1430.75 | 1581.50 | 2161.38 |
Standard Deviation | 251.91 | 301.29 | 323.92 | 478.39 | 261.41 | 311.48 | 362.98 | 555.44 | 262.45 | 328.03 | 343.30 | 545.59 |
Intensity Change Log 2 | — | 0.401477 | 0.339481 | 0.625373 | — | -0.003032 | 0.090424 | 0.697890 | — | 0.205444 | 0.215436 | 0.663509 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001697 | 0.005246 | 0.002181 | 0.009197 | 0.001439 | 0.005247 | 0.005782 | 0.003724 | 0.001555 | 0.005247 | 0.003303 | 0.005628 |
Bud Neck | 0.000223 | 0.005401 | 0.000699 | 0.002357 | 0.000466 | 0.003544 | 0.009851 | 0.000989 | 0.000357 | 0.003800 | 0.003549 | 0.001465 |
Bud Site | 0.000603 | 0.002009 | 0.000659 | 0.005021 | 0.000248 | 0.012817 | 0.023865 | 0.002526 | 0.000407 | 0.011329 | 0.007887 | 0.003394 |
Cell Periphery | 0.000185 | 0.000118 | 0.000067 | 0.000784 | 0.000029 | 0.000852 | 0.001285 | 0.001091 | 0.000099 | 0.000751 | 0.000446 | 0.000984 |
Cytoplasm | 0.000112 | 0.000355 | 0.000117 | 0.000427 | 0.000089 | 0.002430 | 0.002930 | 0.000576 | 0.000099 | 0.002144 | 0.000993 | 0.000524 |
Cytoplasmic Foci | 0.003307 | 0.043653 | 0.004355 | 0.008344 | 0.010088 | 0.019399 | 0.024516 | 0.003108 | 0.007045 | 0.022738 | 0.010634 | 0.004930 |
Eisosomes | 0.000558 | 0.001027 | 0.000414 | 0.001239 | 0.000353 | 0.000454 | 0.000350 | 0.000667 | 0.000445 | 0.000533 | 0.000394 | 0.000866 |
Endoplasmic Reticulum | 0.000081 | 0.000272 | 0.000036 | 0.000589 | 0.000055 | 0.000362 | 0.000611 | 0.000468 | 0.000067 | 0.000350 | 0.000215 | 0.000510 |
Endosome | 0.000573 | 0.007385 | 0.000964 | 0.003413 | 0.001085 | 0.015712 | 0.017635 | 0.001734 | 0.000856 | 0.014566 | 0.006156 | 0.002318 |
Golgi | 0.006479 | 0.050535 | 0.008551 | 0.028192 | 0.013673 | 0.034207 | 0.025059 | 0.030878 | 0.010444 | 0.036455 | 0.013692 | 0.029943 |
Lipid Particles | 0.001336 | 0.008915 | 0.001183 | 0.003572 | 0.003592 | 0.010893 | 0.012090 | 0.001939 | 0.002579 | 0.010621 | 0.004580 | 0.002508 |
Mitochondria | 0.974406* | 0.831546* | 0.967738* | 0.891085* | 0.954320* | 0.853684* | 0.797128* | 0.917721* | 0.963335* | 0.850636* | 0.914601* | 0.908452* |
Mitotic Spindle | 0.000066 | 0.006510 | 0.000478 | 0.003110 | 0.000668 | 0.001373 | 0.008012 | 0.000830 | 0.000398 | 0.002080 | 0.002825 | 0.001623 |
None | 0.001619 | 0.002207 | 0.001382 | 0.002913 | 0.002126 | 0.002250 | 0.002397 | 0.002549 | 0.001898 | 0.002244 | 0.001698 | 0.002676 |
Nuclear Periphery | 0.000081 | 0.000566 | 0.000218 | 0.000464 | 0.000288 | 0.000449 | 0.002977 | 0.000327 | 0.000195 | 0.000465 | 0.001077 | 0.000375 |
Nuclear Periphery Foci | 0.000039 | 0.000695 | 0.000113 | 0.000251 | 0.000150 | 0.000626 | 0.002291 | 0.000163 | 0.000100 | 0.000636 | 0.000792 | 0.000193 |
Nucleolus | 0.000100 | 0.001107 | 0.000168 | 0.000388 | 0.000201 | 0.001333 | 0.002708 | 0.000255 | 0.000156 | 0.001302 | 0.000959 | 0.000301 |
Nucleus | 0.000039 | 0.000212 | 0.000021 | 0.000113 | 0.000029 | 0.000609 | 0.000652 | 0.000176 | 0.000034 | 0.000554 | 0.000217 | 0.000154 |
Peroxisomes | 0.005994 | 0.018468 | 0.003758 | 0.003808 | 0.005124 | 0.011667 | 0.014782 | 0.000775 | 0.005514 | 0.012603 | 0.007191 | 0.001831 |
Vacuole | 0.000598 | 0.008080 | 0.002397 | 0.009882 | 0.001787 | 0.011746 | 0.021840 | 0.009557 | 0.001254 | 0.011241 | 0.008453 | 0.009670 |
Vacuole Periphery | 0.001907 | 0.005694 | 0.004501 | 0.024851 | 0.004190 | 0.010345 | 0.023238 | 0.019948 | 0.003165 | 0.009705 | 0.010337 | 0.021654 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.27 | -0.76 | -5.41 | -1.96 | -4.86 | -4.49 | -5.17 | -3.55 | 1.67 | 2.31 | -5.04 | -3.45 | -6.42 | -0.44 | -3.20 |
Bud Neck | -3.18 | -3.67 | -6.35 | 1.78 | -4.77 | -6.89 | -9.28 | -3.11 | 5.47 | 8.68 | -7.78 | -9.49 | -6.97 | 4.93 | 5.61 |
Bud Site | -1.93 | -0.15 | -3.94 | -2.51 | -4.06 | -7.79 | -7.62 | -4.26 | 5.99 | 6.76 | -7.77 | -7.47 | -5.62 | 5.23 | 3.93 |
Cell Periphery | 0.71 | 1.25 | -2.17 | -2.55 | -2.74 | -5.43 | -4.96 | -4.58 | -0.86 | 0.57 | -4.76 | -3.83 | -4.89 | -1.06 | -2.77 |
Cytoplasm | -4.06 | -0.13 | -3.32 | -0.84 | -3.41 | -4.51 | -2.98 | -1.28 | 2.86 | 2.31 | -4.56 | -3.00 | -1.71 | 3.13 | 1.22 |
Cytoplasmic Foci | -7.78 | -1.77 | -4.26 | 6.65 | -3.30 | -6.23 | -6.46 | 7.68 | 12.36 | 10.07 | -11.25 | -4.10 | 3.04 | 12.78 | 6.49 |
Eisosomes | -2.49 | 1.63 | -4.74 | -0.95 | -5.58 | -2.42 | 0.08 | -4.95 | -3.32 | -5.16 | -1.87 | 0.91 | -6.35 | -4.81 | -6.28 |
Endoplasmic Reticulum | -4.01 | 2.89 | -3.19 | -1.94 | -3.49 | -7.35 | -8.83 | -5.87 | -1.25 | 1.57 | -7.66 | -6.85 | -6.16 | -1.96 | -3.95 |
Endosome | -5.20 | -1.85 | -4.78 | 2.75 | -4.07 | -11.20 | -11.16 | -2.44 | 10.62 | 10.65 | -12.04 | -10.55 | -5.47 | 10.53 | 6.92 |
Golgi | -7.08 | -1.49 | -7.59 | 3.32 | -6.80 | -8.29 | -4.23 | -6.49 | 1.06 | -1.77 | -11.97 | -2.46 | -9.94 | 2.43 | -7.93 |
Lipid Particles | -5.25 | 0.50 | -2.63 | 3.15 | -2.84 | -5.60 | -5.37 | 2.85 | 7.04 | 6.52 | -7.26 | -3.54 | 0.05 | 7.11 | 3.28 |
Mitochondria | 9.42 | 2.03 | 12.47 | -3.63 | 11.62 | 15.16 | 16.30 | 7.40 | -8.81 | -11.96 | 19.13* | 12.17 | 13.99 | -8.79 | 1.27 |
Mitotic Spindle | -1.96 | -4.52 | -3.41 | 0.99 | -2.93 | -1.52 | -5.90 | -0.46 | 1.12 | 5.75 | -2.94 | -5.77 | -3.08 | 0.68 | 2.22 |
None | -2.76 | 2.25 | -7.45 | -2.91 | -9.72 | -0.44 | -0.84 | -2.44 | -0.77 | -0.37 | -1.25 | 1.39 | -6.37 | -1.35 | -7.05 |
Nuclear Periphery | -5.08 | -2.55 | -5.95 | 0.98 | -3.64 | -1.34 | -5.21 | -0.58 | 1.06 | 5.15 | -2.74 | -5.28 | -3.75 | 0.91 | 4.21 |
Nuclear Periphery Foci | -4.57 | -3.31 | -5.14 | 2.95 | -3.08 | -3.87 | -5.09 | -0.43 | 3.79 | 5.06 | -4.97 | -5.18 | -3.79 | 4.09 | 4.45 |
Nucleolus | -2.67 | -1.49 | -5.26 | 1.90 | -4.03 | -3.17 | -3.65 | -1.04 | 3.01 | 3.56 | -3.67 | -3.70 | -3.68 | 3.19 | 3.01 |
Nucleus | -2.15 | 1.06 | -1.80 | 0.97 | -2.42 | -2.90 | -6.69 | -1.85 | 2.04 | 3.74 | -3.00 | -5.96 | -2.26 | 2.21 | 0.85 |
Peroxisomes | -3.44 | 1.70 | 1.61 | 4.20 | -0.06 | -4.54 | -5.02 | 4.90 | 9.38 | 8.12 | -5.41 | -1.73 | 4.84 | 9.55 | 7.57 |
Vacuole | -4.63 | -5.09 | -7.30 | -0.92 | -5.77 | -9.49 | -11.42 | -7.39 | 1.45 | 6.04 | -10.85 | -11.56 | -10.35 | 1.22 | -1.28 |
Vacuole Periphery | -4.96 | -4.36 | -9.56 | -7.76 | -8.41 | -6.22 | -9.61 | -10.74 | -5.92 | 1.38 | -8.08 | -9.36 | -14.64 | -8.36 | -8.05 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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