Standard name
Human Ortholog
Description Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.08
Bud 0.09 0.08 0.11 0.13 0.18 0.2 0.16 0.2 0.23 0.21 0.21 0.21 0.07 0.08 0.06 0.07 0.06 0 0 0 0 0 0.06 0
Bud Neck 0 0.07 0 0 0.05 0.05 0 0 0 0 0 0 0.06 0.06 0.05 0 0 0 0 0.09 0 0.07 0 0
Bud Site 0.16 0.13 0.18 0.15 0.1 0.17 0.12 0.05 0.12 0.09 0.12 0.09 0.13 0.07 0.05 0.08 0.09 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.09 0.07 0.07 0.08 0.09 0.07 0.06 0 0 0.07 0.05 0 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0.05 0 0 0.05 0 0 0 0 0 0 0 0.06 0.09 0.1 0.08 0.06 0.08 0.11 0.12 0.11 0.13 0.12 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 0.36 0.38 0.3 0.25 0.38 0.32 0.34 0.41 0.33 0.52 0.49 0.17 0.07 0 0.09 0.1 0.08 0 0.05 0 0 0.05 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.05 0.08 0.06 0 0.05 0 0 0 0 0 0 0.06 0 0 0.16 0.14 0.15 0 0 0 0 0 0
Peroxisomes 0.57 0.53 0.49 0.54 0.36 0.22 0.31 0.22 0.26 0.38 0.29 0.38 0.26 0.38 0.4 0.56 0.51 0.58 0.59 0.42 0.5 0.44 0.45 0.47
SpindlePole 0.41 0.27 0.42 0.43 0.52 0.46 0.51 0.47 0.44 0.5 0.36 0.35 0.6 0.62 0.65 0.39 0.33 0.38 0.18 0.17 0.26 0.2 0.17 0.1
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 2 1 2 2 2 1 7 2 5 2 8 11 6 4 3 3 1 3 11 3 4 8 20
Bud 14 26 19 25 69 95 117 140 110 146 107 144 15 37 29 17 14 6 3 14 4 7 15 11
Bud Neck 3 21 4 5 19 25 25 14 14 15 11 12 12 27 22 3 9 2 3 32 5 15 10 11
Bud Site 23 39 30 30 40 81 84 33 58 61 61 63 28 34 23 19 21 8 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 3 4 5 34 33 48 59 42 52 29 29 8 32 24 7 16 7 0 2 0 2 3 4
Endoplasmic Reticulum 0 1 0 0 1 0 0 1 1 0 1 1 1 0 3 2 1 1 0 1 0 0 2 1
Endosome 9 17 5 3 19 2 21 27 5 29 6 10 12 43 46 19 15 17 19 41 18 29 32 32
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 3 0 2 2 4
Mitochondria 45 111 63 59 96 183 230 245 200 231 265 342 36 32 16 22 24 16 2 17 2 8 14 19
Nucleus 4 2 2 6 8 8 13 15 8 6 5 7 4 4 3 6 3 6 0 1 0 0 1 0
Nuclear Periphery 1 1 0 0 0 3 2 1 1 2 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 6 16 14 12 11 25 23 29 17 11 10 16 12 9 13 41 34 32 0 5 1 2 1 0
Peroxisomes 85 166 81 105 139 108 223 161 125 271 147 260 55 175 186 139 125 123 101 144 85 96 121 128
SpindlePole 61 85 70 83 199 221 363 339 217 350 184 240 125 288 300 97 79 81 31 57 43 44 45 27
Vac/Vac Membrane 0 4 0 0 2 1 2 2 3 2 2 3 2 3 2 2 1 3 0 1 0 1 2 4
Unique Cell Count 148 311 167 195 382 484 712 716 488 707 511 693 210 466 463 250 243 213 172 342 170 219 267 274
Labelled Cell Count 256 494 293 335 639 787 1152 1074 803 1183 831 1138 321 690 671 378 345 306 172 342 170 219 267 274


Peroxisomes

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.9 4.3 4.1 4.1 3.6 3.3 3.5 3.4 3.4 3.3 3.3 5.7 5.5 5.6 10.3 10.4 11.1 6.4 6.3 6.4
Std Deviation (1e-4) 1.4 1.5 1.0 1.4 1.5 1.1 1.2 1.0 1.2 1.3 1.1 1.1 1.3 1.1 1.1 4.2 4.4 4.1 1.6 1.7 1.9
Intensity Change (Log2) – – – -0.08 -0.07 -0.26 -0.37 -0.31 -0.34 -0.32 -0.36 -0.36 0.41 0.37 0.39 1.27 1.29 1.38 0.57 0.55 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – 0 0 0 0 0 0 0 0 -1.4 -1.3 -2.1 -1.6 -2.1 -3.3
Bud Neck – 0 0 0 0 0 0 0 0 1.6 1.7 1.3 0 0.7 0
Bud Site – 0 0 0 0 0 0 0 0 -1.2 -3.9 -5.2 -3.2 -2.8 -4.6
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 1.3 2.6 2.8 2.0 1.5 2.1
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 -4.5 -9.6 -11.5 -7.2 -6.8 -7.2
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – 0 0 0 0 0 0 0 0 -1.0 -3.8 -3.0 2.4 1.7 2.0
Peroxisomes – 0 0 0 0 0 0 0 0 -4.5 -2.5 -1.9 1.4 0.6 1.8
SpindlePole – 0 0 0 0 0 0 0 0 3.4 4.4 5.1 -0.6 -1.9 -0.8
Vac – – – – – – – – – – – – – – –
Cortical Patches – 0 0 0 0 0 0 0 0 2.5 0 0 0 0 0
Cytoplasm – 2.8 2.1 2.1 2.6 2.7 2.4 1.7 1.1 0.8 2.1 1.5 0 1.9 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0202 1.3297 1.1082 0.8208 0.797 1.2693 0.8105 1.824 1.53 1.3035 1.196 1.798 -0.4505 0.472 -0.1453 0.5369 -0.3187 0.0648
Actin 0.0273 0.0177 0.0114 0.0118 0.003 0.0063 0.0235 0.0155 0.008 0.0011 0.009 0.0015 0.0226 0.0086 0.0147 0.01 0.001 0.0093
Bud 0.003 0.0095 0.0047 0.0003 0.0001 0.0007 0.0003 0.008 0.0033 0.0001 0.0129 0 0.0014 0.002 0.001 0.0003 0 0.0002
Bud Neck 0.0026 0.0151 0.0214 0.0249 0.0115 0.0056 0.0023 0.0217 0.0273 0.0917 0.0061 0.0034 0.0012 0.0192 0.0065 0.0578 0.0087 0.0055
Bud Periphery 0.0035 0.0072 0.0043 0.0003 0.0002 0.0006 0.0003 0.0049 0.01 0.0002 0.0146 0 0.0011 0.0019 0.001 0.0004 0 0.0003
Bud Site 0.0099 0.0926 0.0359 0.0026 0.0009 0.001 0.0095 0.0976 0.0365 0.0007 0.0212 0.0002 0.0078 0.0657 0.015 0.0067 0.0002 0.0008
Cell Periphery 0.0003 0.0005 0.0002 0.0002 0.0001 0.0002 0.0001 0.0012 0.0007 0.0001 0.001 0 0.0002 0.0006 0.0002 0.0001 0 0.0001
Cytoplasm 0.0014 0.002 0.0008 0.0058 0.0012 0.0011 0.007 0.0059 0.0007 0.0005 0.0016 0.0002 0.0043 0.0038 0.0002 0.0011 0.0001 0.0003
Cytoplasmic Foci 0.2822 0.2169 0.2786 0.2359 0.2917 0.2626 0.3359 0.2827 0.2907 0.2945 0.2857 0.3516 0.345 0.329 0.2849 0.2357 0.3186 0.2907
Eisosomes 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.002 0.0003 0.0001 0.0008 0 0.0004 0 0.0001 0 0 0
Endoplasmic Reticulum 0.002 0.0002 0.0001 0.0011 0.0003 0.0001 0.0014 0.001 0.0001 0 0.0001 0 0.001 0 0.0001 0.0002 0 0.0001
Endosome 0.0189 0.0141 0.0087 0.0049 0.0106 0.0084 0.0238 0.0399 0.0152 0.0063 0.0059 0.0093 0.0141 0.0093 0.0063 0.0144 0.0044 0.0129
Golgi 0.0105 0.008 0.0066 0.0071 0.0057 0.0113 0.0084 0.009 0.0076 0.0103 0.0068 0.006 0.0094 0.0066 0.0114 0.0104 0.0069 0.0121
Lipid Particles 0.0779 0.0579 0.0592 0.0859 0.0976 0.1425 0.1047 0.0791 0.0532 0.0706 0.1295 0.1586 0.1355 0.0838 0.1027 0.147 0.1892 0.2116
Mitochondria 0.0079 0.0083 0.0075 0.0035 0.009 0.0094 0.003 0.0078 0.0069 0.0261 0.0033 0.0014 0.0068 0.0089 0.0069 0.0043 0.0033 0.0023
None 0.0028 0.0034 0.0078 0.0018 0.0164 0.0052 0.0387 0.0231 0.0136 0.0528 0.0174 0.0001 0.0175 0.0006 0.0002 0.0012 0.0002 0.0003
Nuclear Periphery 0.0187 0.0002 0.0001 0.007 0.0032 0.0009 0.0023 0.0005 0.0001 0.0001 0.0001 0.0001 0.0042 0.0001 0.0001 0.0007 0 0.0004
Nucleolus 0.0206 0.0176 0.0114 0.016 0.0266 0.0058 0.0166 0.0355 0.0181 0.0279 0.017 0.0016 0.0071 0.0169 0.0125 0.0025 0.0086 0.0114
Nucleus 0.009 0.0044 0.0012 0.0071 0.0037 0.0016 0.0147 0.0031 0.0015 0.0012 0.0009 0.0001 0.0015 0.0133 0.0007 0.0003 0.0002 0.0012
Peroxisomes 0.462 0.4695 0.4817 0.5172 0.4531 0.5088 0.3919 0.3035 0.4833 0.4023 0.4537 0.4315 0.3959 0.4031 0.5161 0.491 0.4529 0.4175
Punctate Nuclear 0.0344 0.0501 0.0569 0.0654 0.0643 0.0254 0.0124 0.0314 0.0196 0.0127 0.0104 0.0338 0.0133 0.0198 0.018 0.0151 0.0053 0.0215
Vacuole 0.0029 0.0043 0.0012 0.001 0.0004 0.0018 0.0023 0.0249 0.0029 0.0003 0.0018 0.0004 0.0086 0.0062 0.0012 0.0006 0.0001 0.0013
Vacuole Periphery 0.002 0.0005 0.0002 0.0002 0.0004 0.0006 0.0008 0.0016 0.0003 0.0003 0.0002 0.0001 0.0011 0.0005 0.0001 0.0002 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.7126 28.9817 27.4382 31.2359 19.5676 25.6983 37.6396 43.6475 32.2175 32.5705
Translational Efficiency 1.082 1.1025 1.1856 0.7663 0.9908 1.3003 0.8594 0.8846 1.064 1.0631

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
Localization
Cell Percentages peroxisome (66%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ant1

Ant1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ant1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available