Standard name | |
---|---|
Human Ortholog | |
Description | Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 4 | 6 | 5 | 6 | 9 | 3 | 13 | 9 | 14 | 0 | 14 | 5 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 2 |
Bud | 2 | 0 | 0 | 0 | 1 | 0 | 4 | 9 | 7 | 2 | 5 | 4 | 0 | 6 | 2 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 0 | 5 | 9 | 14 | 2 | 13 | 8 | 8 | 10 | 8 | 0 | 6 | 3 | 28 | 15 | 16 | 0 | 0 | 0 | 0 | 2 | 0 |
Cytoplasm | 272 | 141 | 31 | 94 | 159 | 161 | 107 | 135 | 115 | 107 | 80 | 101 | 80 | 147 | 130 | 118 | 141 | 116 | 262 | 133 | 52 | 82 | 102 | 156 |
Endoplasmic Reticulum | 12 | 6 | 0 | 4 | 5 | 17 | 24 | 18 | 20 | 22 | 31 | 26 | 6 | 3 | 4 | 1 | 7 | 2 | 27 | 6 | 9 | 21 | 38 | 51 |
Endosome | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 2 | 0 | 5 | 8 | 0 | 1 | 1 | 3 | 0 | 0 | 2 | 2 | 1 |
Golgi | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 0 | 0 | 2 | 2 | 1 | 1 | 2 | 2 | 2 |
Mitochondria | 5 | 1 | 0 | 11 | 29 | 50 | 106 | 98 | 141 | 97 | 115 | 113 | 0 | 0 | 1 | 3 | 7 | 2 | 6 | 0 | 10 | 8 | 13 | 11 |
Nucleus | 0 | 0 | 1 | 2 | 1 | 2 | 3 | 1 | 2 | 1 | 1 | 3 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 1 | 0 | 2 | 17 | 7 | 15 | 17 | 21 | 16 | 4 | 27 | 0 | 1 | 2 | 10 | 5 | 7 | 1 | 6 | 2 | 1 | 8 | 2 |
Unique Cell Count | 288 | 146 | 32 | 109 | 199 | 227 | 218 | 255 | 256 | 230 | 204 | 244 | 85 | 173 | 150 | 149 | 161 | 134 | 311 | 151 | 81 | 123 | 180 | 232 |
Labelled Cell Count | 293 | 152 | 32 | 122 | 229 | 258 | 267 | 306 | 320 | 266 | 255 | 298 | 86 | 192 | 166 | 161 | 179 | 147 | 311 | 151 | 81 | 123 | 180 | 232 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.2 | 11.2 | 8.2 | 8.3 | 8.9 | 7.9 | 7.0 | 7.8 | 6.7 | 7.3 | 6.8 | 7.4 | 8.6 | 10.3 | 10.0 | 17.0 | 17.0 | 16.5 | 9.3 | 9.9 | 10.3 |
Std Deviation (1e-4) | 1.4 | 2.2 | 1.6 | 1.7 | 1.3 | 1.8 | 1.6 | 1.5 | 1.6 | 1.4 | 1.4 | 1.5 | 1.3 | 1.5 | 1.6 | 3.0 | 3.2 | 3.6 | 1.6 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.12 | -0.06 | -0.23 | -0.08 | -0.3 | -0.17 | -0.27 | -0.16 | 0.07 | 0.32 | 0.29 | 1.05 | 1.05 | 1.0 | 0.17 | 0.26 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.7 | -2.3 | -3.1 | -5.1 | -4.7 | -5.5 | -5.3 | -6.1 | -5.9 | -0.6 | -1.8 | -1.6 | -2.4 | -1.5 | -1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.0 | 5.2 | 4.3 | 5.9 | 4.6 | 5.9 | 5.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.1265 | 6.4178 | 6.1391 | 5.2156 | 4.8772 | 6.1001 | 5.008 | 6.0153 | 5.565 | 5.0056 | 5.2746 | 5.4922 | 4.4612 | 6.5703 | 5.5857 | 7.8616 | 5.153 | 5.8894 |
Actin | 0.0399 | 0.0012 | 0.0131 | 0.0049 | 0.0168 | 0.0072 | 0.0031 | 0.0009 | 0.0025 | 0.0007 | 0.0005 | 0.0034 | 0.0148 | 0.0169 | 0.0085 | 0.014 | 0.0022 | 0.0026 |
Bud | 0.0008 | 0.0004 | 0.0016 | 0.0007 | 0.0016 | 0.0006 | 0.0004 | 0.0016 | 0.0005 | 0.0059 | 0.0011 | 0.0007 | 0.0006 | 0.0012 | 0.0013 | 0.0012 | 0.001 | 0.0016 |
Bud Neck | 0.001 | 0.0003 | 0.0007 | 0.0004 | 0.001 | 0.0014 | 0.0004 | 0.0004 | 0.0005 | 0.0016 | 0.0008 | 0.0012 | 0.0005 | 0.0003 | 0.0007 | 0.0009 | 0.0008 | 0.0018 |
Bud Periphery | 0.001 | 0.0001 | 0.0008 | 0.0007 | 0.0016 | 0.0006 | 0.0004 | 0.0006 | 0.0003 | 0.0029 | 0.0003 | 0.0006 | 0.0009 | 0.0004 | 0.001 | 0.0007 | 0.0004 | 0.0008 |
Bud Site | 0.0035 | 0.0034 | 0.0043 | 0.0006 | 0.0039 | 0.0007 | 0.0007 | 0.0059 | 0.0007 | 0.0178 | 0.0052 | 0.001 | 0.0021 | 0.0019 | 0.0058 | 0.0072 | 0.0005 | 0.0004 |
Cell Periphery | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0003 |
Cytoplasm | 0.6008 | 0.8716 | 0.7763 | 0.6839 | 0.5516 | 0.7308 | 0.7385 | 0.8878 | 0.8181 | 0.7266 | 0.8142 | 0.7802 | 0.6987 | 0.8302 | 0.7672 | 0.7476 | 0.6593 | 0.7199 |
Cytoplasmic Foci | 0.0578 | 0.0212 | 0.035 | 0.0458 | 0.0715 | 0.0311 | 0.0321 | 0.0266 | 0.0308 | 0.0261 | 0.0517 | 0.0332 | 0.0256 | 0.0276 | 0.0342 | 0.0246 | 0.0696 | 0.0362 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0 | 0 |
Endoplasmic Reticulum | 0.0573 | 0.0265 | 0.0165 | 0.0188 | 0.0119 | 0.03 | 0.0595 | 0.0174 | 0.0251 | 0.0351 | 0.0192 | 0.0422 | 0.0773 | 0.0183 | 0.017 | 0.0309 | 0.028 | 0.0454 |
Endosome | 0.1253 | 0.054 | 0.1033 | 0.179 | 0.1994 | 0.1306 | 0.1081 | 0.0408 | 0.0811 | 0.1496 | 0.0819 | 0.0905 | 0.0866 | 0.0637 | 0.1041 | 0.0863 | 0.1867 | 0.1214 |
Golgi | 0.0347 | 0.0082 | 0.0164 | 0.0129 | 0.0662 | 0.0257 | 0.0148 | 0.0062 | 0.0135 | 0.009 | 0.0067 | 0.0147 | 0.0188 | 0.0106 | 0.022 | 0.0145 | 0.0312 | 0.0264 |
Lipid Particles | 0.0109 | 0.0002 | 0.0008 | 0.0071 | 0.0111 | 0.0015 | 0.0011 | 0.0002 | 0.0003 | 0.0011 | 0.0002 | 0.0007 | 0.0117 | 0.0064 | 0.0006 | 0.0026 | 0.0002 | 0.0006 |
Mitochondria | 0.0237 | 0.002 | 0.0066 | 0.0033 | 0.0457 | 0.022 | 0.008 | 0.0017 | 0.0063 | 0.0029 | 0.0045 | 0.0158 | 0.0118 | 0.0077 | 0.0223 | 0.0455 | 0.0056 | 0.0287 |
None | 0.0045 | 0.0055 | 0.0099 | 0.0022 | 0.0018 | 0.0015 | 0.0103 | 0.0028 | 0.0089 | 0.0028 | 0.0035 | 0.0033 | 0.006 | 0.0013 | 0.0034 | 0.0014 | 0.0012 | 0.0022 |
Nuclear Periphery | 0.0133 | 0.001 | 0.0016 | 0.002 | 0.0019 | 0.0026 | 0.0081 | 0.0007 | 0.0019 | 0.002 | 0.0011 | 0.0016 | 0.0169 | 0.0018 | 0.0016 | 0.0024 | 0.0029 | 0.0024 |
Nucleolus | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Nucleus | 0.0034 | 0.0006 | 0.0009 | 0.0009 | 0.0014 | 0.0034 | 0.0033 | 0.0011 | 0.001 | 0.002 | 0.0018 | 0.001 | 0.0099 | 0.001 | 0.0012 | 0.0013 | 0.0009 | 0.0018 |
Peroxisomes | 0.0111 | 0.0001 | 0.0049 | 0.0219 | 0.0027 | 0.003 | 0.0003 | 0.0004 | 0.0006 | 0.0002 | 0.0004 | 0.0034 | 0.0034 | 0.0014 | 0.0007 | 0.0018 | 0.0006 | 0.0007 |
Punctate Nuclear | 0.0013 | 0.0001 | 0.0011 | 0.0011 | 0.0006 | 0.0009 | 0.0008 | 0.0002 | 0.0002 | 0.0003 | 0.0007 | 0.0004 | 0.0021 | 0.0002 | 0.0006 | 0.0004 | 0.0003 | 0.0005 |
Vacuole | 0.0058 | 0.0024 | 0.0041 | 0.0085 | 0.0051 | 0.0035 | 0.0072 | 0.0036 | 0.0055 | 0.0091 | 0.0042 | 0.0039 | 0.0078 | 0.0064 | 0.005 | 0.0121 | 0.0052 | 0.0037 |
Vacuole Periphery | 0.0028 | 0.001 | 0.0019 | 0.0051 | 0.0036 | 0.0025 | 0.0024 | 0.0009 | 0.002 | 0.004 | 0.0015 | 0.0021 | 0.0033 | 0.0023 | 0.0023 | 0.0034 | 0.0035 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 65.1534 | 58.2432 | 57.8576 | 63.4911 | 61.9932 | 67.2179 | 65.0635 | 80.4546 | 67.0943 | 67.1546 |
Translational Efficiency | 2.3937 | 2.8085 | 2.1677 | 2.01 | 2.1691 | 2.4226 | 1.9655 | 1.8853 | 1.9747 | 1.841 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2728 | 951 | 2983 | 1901 | 1537 | 1241 | 2228 | 1302 | 4265 | 2192 | 5211 | 3203 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1063.14 | 1076.62 | 1175.93 | 1314.21 | 981.07 | 1054.41 | 1224.26 | 1186.63 | 1033.56 | 1064.05 | 1196.59 | 1262.35 |
Standard Deviation | 130.19 | 154.63 | 160.33 | 210.04 | 133.00 | 147.76 | 160.58 | 199.98 | 137.00 | 151.18 | 162.21 | 215.33 |
Intensity Change Log 2 | — | 0.018178 | 0.145471 | 0.305864 | — | 0.104008 | 0.319482 | 0.274442 | — | 0.060007 | 0.231605 | 0.290869 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000282 | 0.000673 | 0.000599 | 0.000931 | 0.000254 | 0.000784 | 0.000408 | 0.000345 | 0.000272 | 0.000735 | 0.000517 | 0.000693 |
Bud Neck | 0.001020 | 0.001186 | 0.002252 | 0.001530 | 0.001201 | 0.001695 | 0.001553 | 0.003040 | 0.001085 | 0.001474 | 0.001953 | 0.002144 |
Bud Site | 0.013833 | 0.038307 | 0.020989 | 0.034241 | 0.015271 | 0.033735 | 0.019184 | 0.016868 | 0.014351 | 0.035719 | 0.020217 | 0.027179 |
Cell Periphery | 0.000266 | 0.000344 | 0.000376 | 0.001277 | 0.000305 | 0.001485 | 0.000312 | 0.000118 | 0.000280 | 0.000990 | 0.000348 | 0.000806 |
Cytoplasm | 0.496311* | 0.437302* | 0.660799* | 0.695908* | 0.388476* | 0.447812* | 0.521314* | 0.412186* | 0.457450* | 0.443252* | 0.601161* | 0.580577* |
Cytoplasmic Foci | 0.303583* | 0.350682* | 0.095631 | 0.084548 | 0.352802* | 0.343064* | 0.137499 | 0.114204 | 0.321320* | 0.346369* | 0.113532 | 0.096603 |
Eisosomes | 0.000070 | 0.000030 | 0.000022 | 0.000014 | 0.000060 | 0.000053 | 0.000012 | 0.000011 | 0.000067 | 0.000043 | 0.000018 | 0.000013 |
Endoplasmic Reticulum | 0.027523 | 0.022220 | 0.006606 | 0.006536 | 0.049516 | 0.016664 | 0.017871 | 0.004642 | 0.035449 | 0.019075 | 0.011422 | 0.005766 |
Endosome | 0.016055 | 0.026926 | 0.016376 | 0.013482 | 0.027570 | 0.023279 | 0.031794 | 0.040979 | 0.020205 | 0.024861 | 0.022968 | 0.024660 |
Golgi | 0.064223 | 0.044052 | 0.012824 | 0.022078 | 0.048731 | 0.049495 | 0.013799 | 0.011358 | 0.058640 | 0.047133 | 0.013241 | 0.017720 |
Lipid Particles | 0.006195 | 0.002987 | 0.009028 | 0.012134 | 0.006185 | 0.004546 | 0.007454 | 0.005500 | 0.006191 | 0.003869 | 0.008355 | 0.009437 |
Mitochondria | 0.008504 | 0.001993 | 0.001212 | 0.000867 | 0.006585 | 0.004718 | 0.000638 | 0.002964 | 0.007813 | 0.003536 | 0.000967 | 0.001720 |
Mitotic Spindle | 0.000431 | 0.002029 | 0.003386 | 0.005589 | 0.000456 | 0.003038 | 0.001461 | 0.015818 | 0.000440 | 0.002600 | 0.002563 | 0.009747 |
None | 0.001797 | 0.000775 | 0.000406 | 0.000142 | 0.000743 | 0.000947 | 0.000128 | 0.001518 | 0.001417 | 0.000873 | 0.000287 | 0.000701 |
Nuclear Periphery | 0.000091 | 0.000119 | 0.000361 | 0.000072 | 0.000184 | 0.000095 | 0.000335 | 0.000630 | 0.000124 | 0.000105 | 0.000350 | 0.000299 |
Nuclear Periphery Foci | 0.000459 | 0.000970 | 0.001498 | 0.002801 | 0.000636 | 0.000719 | 0.002684 | 0.003809 | 0.000523 | 0.000828 | 0.002005 | 0.003211 |
Nucleolus | 0.000143 | 0.000236 | 0.000319 | 0.000173 | 0.000124 | 0.000217 | 0.000162 | 0.001114 | 0.000137 | 0.000226 | 0.000252 | 0.000555 |
Nucleus | 0.003525 | 0.001869 | 0.006879 | 0.002454 | 0.003261 | 0.002626 | 0.004173 | 0.009678 | 0.003430 | 0.002297 | 0.005722 | 0.005391 |
Peroxisomes | 0.000761 | 0.000943 | 0.001010 | 0.001189 | 0.000759 | 0.001579 | 0.000671 | 0.001454 | 0.000761 | 0.001303 | 0.000865 | 0.001297 |
Vacuole | 0.024633 | 0.042302 | 0.147326 | 0.098144 | 0.051670 | 0.041094 | 0.219038* | 0.335797* | 0.034376 | 0.041618 | 0.177987 | 0.194749 |
Vacuole Periphery | 0.030294 | 0.024055 | 0.012101 | 0.015889 | 0.045213 | 0.022355 | 0.019511 | 0.017966 | 0.035670 | 0.023093 | 0.015269 | 0.016733 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.91 | -3.50 | -4.11 | -1.45 | -1.86 | -5.23 | -4.11 | -1.24 | 3.62 | 0.93 | -6.41 | -4.53 | -4.27 | 0.30 | -1.57 |
Bud Neck | -1.67 | -8.21 | -6.22 | -3.70 | 4.03 | -3.10 | -1.84 | -7.11 | -5.37 | -5.37 | -4.07 | -7.30 | -9.03 | -5.73 | -2.27 |
Bud Site | -11.40 | -6.91 | -12.97 | 1.68 | -7.72 | -9.47 | -3.34 | -0.74 | 7.64 | 1.67 | -14.90 | -7.57 | -11.13 | 5.15 | -5.52 |
Cell Periphery | -2.87 | -3.79 | -6.86 | -6.21 | -6.03 | -1.55 | -0.10 | 10.48 | 1.81 | 8.42 | -1.65 | -3.26 | -5.85 | 0.50 | -5.02 |
Cytoplasm | 5.32 | -21.46 | -23.82 | -22.06 | -4.22 | -5.47 | -13.78 | -2.51 | 2.63 | 9.69 | 1.86 | -23.43 | -17.26 | -16.22 | 2.75 |
Cytoplasmic Foci | -5.46 | 44.63 | 44.69 | 33.14 | 3.52 | 1.16 | 33.85 | 36.28 | 31.25 | 5.80 | -4.21 | 54.64 | 56.78 | 46.70 | 6.79 |
Eisosomes | 7.72 | 10.72 | 13.35 | 4.94 | 3.66 | 1.14 | 13.03 | 13.13 | 7.62 | 1.43 | 5.28 | 15.59 | 17.89 | 8.82 | 3.87 |
Endoplasmic Reticulum | 1.87 | 13.03 | 12.30 | 6.36 | 0.22 | 9.20 | 9.21 | 14.09 | 7.48 | 9.28 | 7.99 | 14.64 | 18.75 | 9.32 | 7.00 |
Endosome | -7.25 | -0.55 | 3.21 | 8.68 | 3.56 | 3.00 | -2.84 | -4.98 | -7.11 | -2.94 | -4.78 | -3.72 | -3.10 | 1.43 | -0.25 |
Golgi | 8.52 | 36.29* | 23.79 | 9.95 | -6.71 | -0.33 | 22.76 | 22.27 | 19.16 | 3.06 | 6.97 | 42.39 | 32.14 | 19.57 | -3.78 |
Lipid Particles | 8.71 | -5.11 | -6.77 | -10.77 | -3.28 | 3.37 | -2.82 | 0.91 | -1.21 | 2.63 | 7.19 | -5.77 | -5.38 | -9.17 | -1.68 |
Mitochondria | 13.81 | 17.71 | 17.07 | 3.73 | 1.65 | 2.06 | 10.66 | 4.88 | 2.29 | -2.85 | 8.01 | 20.59 | 14.61 | 3.97 | -1.88 |
Mitotic Spindle | -2.12 | -5.00 | -4.59 | -2.74 | -1.77 | -2.73 | -4.53 | -7.25 | -5.86 | -6.82 | -3.44 | -6.04 | -8.55 | -6.13 | -6.53 |
None | 2.10 | 3.74 | 4.65 | 1.93 | 2.23 | -0.45 | 6.97 | -1.02 | -0.52 | -2.15 | 1.48 | 4.74 | 2.50 | 0.71 | -1.43 |
Nuclear Periphery | -0.77 | -2.58 | 0.48 | 1.26 | 2.76 | 2.01 | -1.93 | -6.20 | -7.23 | -3.30 | 0.57 | -3.29 | -5.33 | -5.49 | 0.38 |
Nuclear Periphery Foci | -3.39 | -6.21 | -7.62 | -5.74 | -3.96 | -0.58 | -8.05 | -7.12 | -6.76 | -2.40 | -2.84 | -10.17 | -10.43 | -8.98 | -4.37 |
Nucleolus | -0.70 | -4.74 | -1.23 | 0.49 | 3.06 | -1.34 | -3.74 | -4.17 | -3.61 | -4.01 | -1.21 | -5.34 | -4.30 | -2.48 | -3.04 |
Nucleus | 7.93 | -10.77 | 1.65 | -3.00 | 9.32 | 2.72 | -4.08 | -9.32 | -10.08 | -8.09 | 7.41 | -11.31 | -7.09 | -9.73 | -0.92 |
Peroxisomes | -2.90 | -4.52 | -4.46 | -2.24 | -1.74 | -4.07 | 1.52 | -4.74 | 0.31 | -5.22 | -4.63 | -2.84 | -6.51 | -0.07 | -5.05 |
Vacuole | -5.85 | -30.63 | -22.76 | -13.85 | 8.97 | 2.87 | -30.21 | -35.50 | -36.95 | -13.28 | -3.30 | -43.61 | -37.70 | -34.08 | -3.87 |
Vacuole Periphery | 3.25 | 14.65 | 10.11 | 4.71 | -2.92 | 8.83 | 11.12 | 12.17 | 3.80 | 2.55 | 8.43 | 17.56 | 15.67 | 5.99 | -0.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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