Standard name
Human Ortholog
Description Catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.98 1.0 0.93 0.95 0.91 0.92 0.76 0.8 0.76 0.78 0.99 0.99 0.99 0.96 0.93 0.95 0.94 0.97 0.94 0.87 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.06 0.11 0.07 0.42 0.25 0.4 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 1 0 6 2 0 2 0 0 0 0 2 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 7 0 2 0 2 3 5 4 5 3 3 4 7 5 16 12 11 0 0 0 0 0 0
Cytoplasm 151 226 79 80 65 112 143 162 164 162 114 137 204 345 269 238 261 193 152 221 82 65 185 174
Endoplasmic Reticulum 0 0 0 0 1 1 0 0 1 3 0 4 0 1 0 8 9 7 0 0 0 0 0 2
Endosome 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 1 1 3 0 0 3 1
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 0 0 0 0 2 0
Mitochondria 6 0 0 0 4 7 17 13 90 50 60 52 1 1 0 11 8 0 0 1 1 0 3 4
Nucleus 2 0 2 0 4 1 3 3 0 1 2 3 0 1 0 1 5 5 0 0 0 1 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 0 0 2 3 8 3 5 0 5 1 0 1 3 14 7 2 0 1 0 2 0
Unique Cell Count 154 227 81 80 70 118 157 177 215 202 150 175 206 347 271 248 281 204 163 229 87 75 207 192
Labelled Cell Count 160 234 82 82 76 125 171 191 269 231 180 206 211 356 278 279 313 226 163 229 87 75 207 192


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.2 6.3 6.8 4.8 5.7 5.7 6.0 4.7 5.2 4.7 5.0 7.2 7.1 8.1 7.2 8.8 9.3 8.1 8.1 8.2
Std Deviation (1e-4) 1.2 0.8 0.9 1.3 1.3 0.9 1.7 1.6 1.0 1.0 0.8 1.2 1.0 1.1 14.2 1.4 1.6 1.7 1.4 1.4 1.3
Intensity Change (Log2) – – – 0.11 -0.4 -0.13 -0.14 -0.07 -0.4 -0.26 -0.4 -0.33 0.2 0.18 0.37 0.21 0.49 0.57 0.38 0.37 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 -1.4 -0.9 -1.9 -1.8 -4.3 -3.7 -4.2 -3.9 1.0 1.6 1.3 -0.7 -1.5 -1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 0 0 4.9 0 5.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9428 1.4163 1.0423 0.8126 0.4268 1.1124 6.3187 6.5384 6.4116 5.8924 6.3722 6.2722 2.6845 3.3212 3.0411 2.7994 2.4172 2.9929
Actin 0.0361 0.0014 0.02 0.0021 0.0082 0.0026 0.028 0.0027 0.0186 0.0067 0.0005 0.0129 0.0149 0.0003 0.01 0.0019 0.0074 0.0008
Bud 0.0018 0.0013 0.0024 0.0012 0.0006 0.0014 0.0032 0.0051 0.0036 0.0009 0.0004 0.0023 0.0008 0.0019 0.0005 0.0022 0.0046 0.0011
Bud Neck 0.0051 0.0002 0.0007 0.0004 0.0011 0.0008 0.0124 0.0003 0.0048 0.0005 0.0002 0.0015 0.0024 0.0002 0.0002 0.0106 0.0004 0.0049
Bud Periphery 0.0023 0.0023 0.0021 0.0017 0.0007 0.0016 0.0108 0.0038 0.0064 0.0009 0.0002 0.0044 0.0018 0.0018 0.0008 0.0046 0.0072 0.0042
Bud Site 0.0051 0.0039 0.0044 0.0003 0.0008 0.0012 0.0116 0.0269 0.0094 0.0015 0.0007 0.001 0.002 0.0087 0.0023 0.002 0.0139 0.0001
Cell Periphery 0.0006 0.0002 0.0003 0.0001 0.0001 0.0001 0.0024 0.0006 0.0008 0.0002 0.0001 0.0003 0.0018 0.0005 0.0005 0.0031 0.0002 0.0002
Cytoplasm 0.5945 0.8194 0.633 0.8072 0.6844 0.7928 0.5423 0.7478 0.6346 0.7192 0.7622 0.7296 0.5113 0.673 0.6557 0.5751 0.7072 0.6591
Cytoplasmic Foci 0.0229 0.0113 0.0178 0.0152 0.0223 0.0199 0.0409 0.0086 0.0242 0.0239 0.0069 0.0158 0.0165 0.0053 0.0084 0.0106 0.0132 0.0063
Eisosomes 0.0005 0.0001 0.0007 0 0.0001 0 0.0003 0.0001 0.0004 0.0001 0 0.0001 0.0005 0.0001 0.0001 0.0011 0.0001 0
Endoplasmic Reticulum 0.0085 0.002 0.0038 0.0025 0.0032 0.0024 0.0081 0.0025 0.003 0.0061 0.0027 0.0044 0.0091 0.0024 0.0024 0.0025 0.003 0.0027
Endosome 0.0453 0.0062 0.0232 0.0093 0.0132 0.0132 0.031 0.0021 0.0219 0.0269 0.0299 0.0118 0.015 0.0013 0.008 0.0045 0.0084 0.0051
Golgi 0.012 0.0007 0.0045 0.0008 0.0064 0.0024 0.0107 0.0005 0.0049 0.0053 0.0018 0.0075 0.003 0.0002 0.0018 0.001 0.0029 0.0004
Lipid Particles 0.0124 0.0005 0.0057 0.0003 0.0005 0.0021 0.0113 0.0002 0.0022 0.0124 0.0002 0.0023 0.0039 0.0002 0.0021 0.0009 0.0007 0.0001
Mitochondria 0.0064 0.0005 0.0022 0.0009 0.0553 0.0013 0.0023 0.0003 0.0017 0.0096 0.0022 0.0058 0.0033 0.0005 0.0006 0.0091 0.0015 0.0006
None 0.1992 0.1451 0.2663 0.1509 0.1857 0.1519 0.2531 0.1915 0.2519 0.1684 0.1486 0.1925 0.3834 0.3 0.2977 0.3587 0.2215 0.3126
Nuclear Periphery 0.0126 0.0005 0.0022 0.0014 0.0008 0.0006 0.0069 0.0004 0.0011 0.0051 0.0157 0.0012 0.0042 0.0003 0.0012 0.0007 0.0005 0.0003
Nucleolus 0.0017 0.0001 0.0003 0.0001 0.0002 0.0001 0.0008 0.0003 0.0002 0.0004 0.0002 0.0001 0.0004 0.0001 0.0003 0.0009 0.0001 0
Nucleus 0.0052 0.0011 0.0016 0.0012 0.0018 0.0011 0.0058 0.002 0.001 0.002 0.0215 0.0009 0.0035 0.0013 0.0014 0.0017 0.0006 0.0004
Peroxisomes 0.0109 0.0004 0.0027 0.0008 0.0079 0.0013 0.0062 0.0011 0.0049 0.0023 0.0002 0.0024 0.0143 0.0001 0.0021 0.0023 0.0045 0.0001
Punctate Nuclear 0.0078 0.0004 0.002 0.0013 0.0045 0.0011 0.0033 0.0011 0.001 0.0025 0.0003 0.0012 0.0018 0.0003 0.0017 0.0008 0.0006 0.0001
Vacuole 0.0066 0.0022 0.0032 0.0018 0.0018 0.0015 0.0073 0.0021 0.0027 0.0036 0.0037 0.0016 0.005 0.0015 0.0022 0.0054 0.0012 0.0007
Vacuole Periphery 0.0025 0.0002 0.0007 0.0004 0.0005 0.0003 0.0012 0.0001 0.0006 0.0017 0.0017 0.0005 0.0009 0.0001 0.0003 0.0006 0.0003 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.8888 18.4526 13.6926 24.1927 24.3301 9.3775 20.3535 28.6193 23.6037 28.2338
Translational Efficiency 2.5749 2.4428 2.9875 1.6873 1.8041 4.4577 2.0774 1.4649 1.8634 1.5556

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1209 446 2570 1235 2043 1751 1913 1089 3252 2197 4483 2324

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.12 892.59 911.64 926.91 778.88 919.90 891.42 913.08 749.23 914.36 903.01 920.43
Standard Deviation 86.38 112.20 110.71 166.25 102.53 115.94 126.13 154.24 104.23 115.71 117.96 160.88
Intensity Change Log 2 — 0.352458 0.382924 0.406889 — 0.240076 0.194704 0.229340 — 0.294327 0.286801 0.316052

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000381 0.000631 0.000067 0.002180 0.000090 0.000260 0.000079 0.001054 0.000198 0.000335 0.000072 0.001653
Bud Neck 0.023353 0.022247 0.002838 0.010675 0.004685 0.017543 0.002456 0.003498 0.011626 0.018498 0.002675 0.007312
Bud Site 0.033545 0.063662 0.005408 0.069120 0.007592 0.023799 0.006825 0.032975 0.017240 0.031891 0.006013 0.052183
Cell Periphery 0.000310 0.000149 0.000035 0.000130 0.000112 0.000114 0.000062 0.000372 0.000186 0.000121 0.000047 0.000244
Cytoplasm 0.504197* 0.430784* 0.786778* 0.440402* 0.708534* 0.593070* 0.693873* 0.644117* 0.632567* 0.560126* 0.747134* 0.535861*
Cytoplasmic Foci 0.301116* 0.279897* 0.018629 0.045963 0.168031 0.179728 0.035271 0.047055 0.217508* 0.200063* 0.025730 0.046475
Eisosomes 0.000292 0.000084 0.000012 0.000028 0.000053 0.000079 0.000025 0.000037 0.000142 0.000080 0.000018 0.000032
Endoplasmic Reticulum 0.001577 0.000684 0.000411 0.001036 0.000998 0.000359 0.000501 0.000501 0.001213 0.000425 0.000449 0.000785
Endosome 0.022269 0.039905 0.001963 0.031951 0.006768 0.025841 0.003931 0.004422 0.012531 0.028696 0.002803 0.019052
Golgi 0.015401 0.008703 0.000098 0.022348 0.002752 0.007185 0.000772 0.000742 0.007455 0.007494 0.000385 0.012224
Lipid Particles 0.005591 0.001694 0.000466 0.001382 0.001947 0.001449 0.000578 0.000529 0.003302 0.001499 0.000514 0.000982
Mitochondria 0.016216 0.001158 0.000163 0.008330 0.000912 0.003840 0.000485 0.000188 0.006601 0.003295 0.000300 0.004515
Mitotic Spindle 0.001474 0.003324 0.007394 0.134411* 0.000855 0.004898 0.009661 0.012019 0.001085 0.004579 0.008361 0.077059
None 0.002112 0.000221 0.002968 0.001465 0.003618 0.000943 0.002992 0.003016 0.003058 0.000796 0.002978 0.002192
Nuclear Periphery 0.000148 0.000069 0.000446 0.000793 0.000088 0.000085 0.000342 0.000244 0.000110 0.000081 0.000402 0.000536
Nuclear Periphery Foci 0.000716 0.000747 0.000710 0.001539 0.000411 0.000196 0.000966 0.001304 0.000524 0.000308 0.000819 0.001428
Nucleolus 0.000707 0.000222 0.000163 0.000655 0.000279 0.000312 0.000296 0.000282 0.000438 0.000294 0.000220 0.000480
Nucleus 0.018507 0.015946 0.089674 0.025056 0.044009 0.018980 0.077557 0.038806 0.034528 0.018364 0.084504 0.031499
Peroxisomes 0.004904 0.009478 0.000170 0.003106 0.000807 0.004494 0.000297 0.000460 0.002330 0.005505 0.000224 0.001866
Vacuole 0.041589 0.117722 0.081484 0.187242 0.046266 0.113741 0.162539 0.207933* 0.044527 0.114549 0.116072 0.196938
Vacuole Periphery 0.005594 0.002671 0.000123 0.012187 0.001192 0.003083 0.000490 0.000446 0.002829 0.003000 0.000279 0.006685

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.30 8.49 -1.20 -0.08 -2.66 -5.10 -0.47 -1.40 -0.76 -1.37 -3.72 7.27 -2.13 -1.33 -2.80
Bud Neck 0.48 12.70 11.12 6.27 -2.15 -10.77 5.74 4.52 12.42 -0.51 -5.40 13.14 10.84 13.02 -2.12
Bud Site -4.98 13.60 3.48 6.71 -7.50 -11.17 1.89 -5.33 2.30 -6.00 -7.96 12.51 -1.90 4.96 -9.64
Cell Periphery 6.52 13.46 11.78 5.52 -4.83 -0.06 11.23 -0.72 -0.68 -1.61 4.17 16.98 2.45 0.36 -2.09
Cytoplasm 4.70 -22.48 7.44 0.90 28.69 14.23 9.51 16.95 5.02 8.95 9.63 -7.22 20.42 9.81 26.98
Cytoplasmic Foci 1.93 47.32 45.46 24.33 -2.55 -1.95 35.10 31.50 30.45 -3.07 3.47 55.80 52.05 39.71 -4.36
Eisosomes 9.72 13.44 12.93 10.05 -9.49 -4.76 11.35 6.61 8.39 -4.41 6.88 15.14 13.83 11.78 -9.28
Endoplasmic Reticulum 3.68 4.54 4.23 0.60 -0.50 2.58 1.84 1.98 -2.48 0.53 4.44 3.74 3.67 -2.38 -0.07
Endosome -5.31 15.80 8.26 9.91 -5.54 -13.93 9.08 8.95 16.82 1.04 -11.96 18.48 8.43 16.70 -5.08
Golgi 4.04 20.56 8.29 1.99 -4.57 -6.12 6.19 11.51 9.52 0.42 0.12 21.01 7.41 5.51 -4.28
Lipid Particles 4.85 6.67 5.87 1.84 -5.17 1.68 5.35 5.18 5.37 -0.94 5.17 8.41 7.33 4.22 -5.15
Mitochondria 8.60 9.29 8.04 -1.73 -4.65 -3.97 2.35 4.81 5.15 1.17 3.85 9.52 7.89 3.50 -3.94
Mitotic Spindle -0.75 -4.76 -10.13 -8.76 -8.38 -3.07 -5.14 -4.97 -2.55 -0.55 -3.08 -7.08 -11.15 -9.20 -7.80
None 2.45 -0.89 0.17 -11.14 4.31 4.57 1.82 2.29 -2.64 0.75 4.57 0.77 2.09 -3.97 2.61
Nuclear Periphery 1.98 -13.52 -10.62 -12.58 -4.10 0.19 -19.34 -15.17 -14.04 1.12 1.40 -24.13 -14.71 -15.59 -3.32
Nuclear Periphery Foci 0.34 0.22 -0.34 -1.95 -2.28 6.08 -6.82 -7.70 -9.51 -2.46 1.44 -1.39 -2.85 -9.92 -3.43
Nucleolus 2.25 2.40 1.58 -2.54 -3.31 -0.75 -0.25 0.56 0.93 0.59 1.68 2.39 1.48 -0.29 -1.55
Nucleus 1.19 -33.67 -13.55 -12.74 19.87 15.56 -15.71 -2.36 -11.89 10.62 12.68 -33.51 -8.77 -17.86 22.14
Peroxisomes -3.21 14.52 10.72 6.31 -3.44 -10.96 8.31 9.12 12.38 -1.17 -7.76 16.22 10.44 12.30 -3.68
Vacuole -11.58 -21.88 -39.80 -25.90 -28.52 -18.34 -30.66 -32.39 -23.17 -11.14 -21.96 -36.57 -50.89 -36.40 -28.83
Vacuole Periphery 4.32 10.35 3.74 -0.10 -4.55 -5.17 5.86 7.96 8.11 0.91 -0.36 12.15 3.47 3.38 -4.36
Standard name
Human Ortholog
Description Catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Nat3

Nat3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nat3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available