Standard name
Human Ortholog
Description Ammonium permease of high capacity and low affinity; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation ammonia permease activity regulated by TORC1 effectors, Npr1p and Par32p; MEP3 has a paralog, MEP1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.33 0.28 0.31 0.34 0.4 0.31 0.22 0.18 0.14 0.07 0.1 0.06 0.29 0.31 0.27 0 0.07 0 0 0 0 0.06 0 0
Bud 0 0.05 0 0 0.07 0.09 0.1 0.1 0.12 0.06 0.07 0.08 0.06 0.06 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.91 0.84 0.86 0.81 0.72 0.52 0.44 0.27 0.29 0.27 0.23 0.17 0.82 0.82 0.76 0.56 0.42 0.12 0.93 0.81 0.92 0.8 0.9 0.87
Cytoplasm 0.05 0.13 0.05 0.09 0.1 0.37 0.45 0.58 0.58 0.71 0.7 0.74 0.15 0.15 0.2 0.44 0.42 0.75 0 0.06 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.11 0.13 0.12 0.1 0.09 0.09 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0.21 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0.05 0.06 0.06 0.11 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 57 56 96 72 106 66 62 56 36 23 26 22 81 86 87 0 3 0 2 7 10 21 16 12
Bud 2 10 9 8 19 19 30 31 29 18 18 28 17 17 11 0 0 0 0 3 1 3 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 3 9
Bud Site 0 0 0 1 0 0 1 2 3 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 159 169 265 171 191 110 128 84 74 85 56 59 229 229 246 9 19 2 194 192 311 279 459 435
Cytoplasm 8 27 16 19 27 77 131 176 146 227 174 258 42 42 63 7 19 12 2 15 0 8 3 6
Endoplasmic Reticulum 1 6 8 6 29 27 35 31 23 28 18 26 7 7 13 0 0 0 4 2 7 13 11 10
Endosome 0 0 2 0 1 0 0 7 3 2 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 1 2 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0
Mitochondria 10 8 64 21 4 4 6 4 5 7 6 6 3 3 4 0 4 1 0 3 2 8 6 8
Nucleus 1 1 0 0 0 0 3 3 6 9 5 5 1 1 0 0 4 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 1 6 7 5 5 3 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 0 2 0 2 2 2 0 1 0 2 1 5 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 0 0 3 4 2 25 13 19 15 40 2 3 3 0 1 1 0 4 0 3 1 3
Unique Cell Count 175 202 308 211 265 210 288 306 251 318 248 350 280 279 322 16 45 16 208 236 339 348 508 501
Labelled Cell Count 239 279 462 305 391 312 405 428 341 422 320 449 384 389 434 16 50 16 208 236 339 348 508 501


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.9 14.5 14.0 14.2 12.6 10.4 10.3 10.6 10.3 9.7 9.7 9.7 12.2 12.2 12.1 12.7 13.7 14.8 13.6 14.4 15.1
Std Deviation (1e-4) 2.4 3.1 2.8 2.6 3.0 3.4 2.8 2.8 2.7 2.6 2.4 2.2 2.3 2.3 2.4 3.5 4.4 3.9 2.8 2.7 3.0
Intensity Change (Log2) – – – 0.02 -0.15 -0.43 -0.44 -0.4 -0.45 -0.52 -0.52 -0.53 -0.19 -0.19 -0.2 -0.14 -0.03 0.08 -0.04 0.04 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.7 2.2 0.1 -2.7 -3.7 -4.7 -7.6 -5.9 -8.3 -0.6 -0.1 -1.1 0 -3.4 0
Bud 0.5 2.4 3.0 3.7 3.6 4.0 1.7 2.4 2.8 1.9 1.9 0.4 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.5 -4.1 -8.4 -10.7 -14.7 -13.6 -14.9 -15.1 -17.7 -1.4 -1.3 -3.1 -3.2 -6.9 -7.5
Cytoplasm 1.7 2.3 9.2 11.4 14.0 13.7 17.0 16.1 17.8 4.0 4.0 5.4 0 0 0
Endoplasmic Reticulum 0.2 4.1 4.6 4.5 3.8 3.4 3.3 2.6 2.8 -0.1 -0.1 1.0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -3.3 -7.1 -6.2 -7.1 -7.7 -6.7 -7.3 -6.5 -7.9 -7.5 -7.5 -7.9 0 -1.9 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 5.1 4.0 4.4 4.4 6.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.881 6.9849 5.5839 5.9817 5.7632 6.0537 5.4681 7.9038 6.8542 6.4591 6.0181 7.3222 5.8914 9.0088 7.9068 8.16 7.0456 8.4511
Actin 0.0226 0.0105 0.0188 0.0081 0.0139 0.0088 0.0605 0.0063 0.015 0.0014 0.017 0.0063 0.0402 0.0147 0.0105 0.0012 0.0466 0.0014
Bud 0.0016 0.0005 0.002 0.0008 0.0012 0.0009 0.0005 0.0006 0.0005 0.0002 0.0013 0.0005 0.0006 0.0004 0.0007 0.0005 0.0005 0.0003
Bud Neck 0.0031 0.0022 0.0065 0.0047 0.0107 0.0056 0.0037 0.0018 0.0081 0.0025 0.0087 0.0042 0.0078 0.0047 0.0121 0.003 0.0016 0.003
Bud Periphery 0.0155 0.005 0.0114 0.0122 0.0137 0.0101 0.0065 0.0046 0.0059 0.0045 0.0216 0.009 0.006 0.0028 0.0094 0.0106 0.0143 0.0084
Bud Site 0.0127 0.0265 0.026 0.0048 0.0049 0.0035 0.01 0.0308 0.0292 0.0032 0.0046 0.0021 0.0216 0.0485 0.0191 0.0024 0.0013 0.0008
Cell Periphery 0.663 0.6772 0.6941 0.7504 0.7141 0.7807 0.7317 0.8331 0.7979 0.8732 0.8358 0.8892 0.7169 0.7763 0.8468 0.9255 0.8853 0.9179
Cytoplasm 0.1069 0.213 0.1645 0.1738 0.1493 0.115 0.0577 0.0813 0.0684 0.0617 0.0464 0.0587 0.0712 0.1318 0.0849 0.0423 0.0306 0.04
Cytoplasmic Foci 0.0089 0.0013 0.0037 0.0007 0.0012 0.0026 0.004 0.0004 0.0027 0.0001 0.0035 0.0013 0.0045 0.0022 0.0004 0.0001 0.0002 0.0001
Eisosomes 0.0369 0.0377 0.0242 0.0225 0.0155 0.0297 0.0798 0.0305 0.0369 0.0432 0.0266 0.0131 0.0903 0.0038 0.0069 0.0042 0.0081 0.0195
Endoplasmic Reticulum 0.0134 0.0153 0.0114 0.0095 0.0218 0.0121 0.0054 0.0038 0.0031 0.0069 0.0045 0.0035 0.0108 0.005 0.0038 0.0048 0.0073 0.0044
Endosome 0.0157 0.0004 0.0048 0.0003 0.0063 0.0042 0.0013 0.0001 0.0006 0 0.0073 0.0011 0.0023 0.0003 0.0001 0 0 0
Golgi 0.0093 0.0028 0.0026 0.0013 0.0038 0.0057 0.004 0.0006 0.0023 0.0001 0.0026 0.0026 0.0076 0.0017 0.0004 0.0002 0.0003 0.0001
Lipid Particles 0.0149 0.0021 0.0048 0.0009 0.0043 0.0055 0.0058 0.0004 0.0023 0.0002 0.0037 0.001 0.0038 0.0044 0.0005 0.0005 0.0003 0.0006
Mitochondria 0.0202 0.0006 0.003 0.0011 0.0122 0.0028 0.0036 0.0003 0.0037 0.0001 0.001 0.0009 0.003 0.0001 0.0002 0.0001 0.0001 0
None 0.0133 0.0019 0.0044 0.005 0.0165 0.0013 0.0159 0.0019 0.0129 0.0007 0.0025 0.001 0.0048 0.0004 0.0006 0.0004 0.0007 0.0005
Nuclear Periphery 0.0091 0.0006 0.0041 0.0004 0.0024 0.0008 0.0006 0.0003 0.0003 0.0005 0.0039 0.0002 0.001 0.0003 0.0004 0.0003 0.0003 0.0003
Nucleolus 0.0022 0.0001 0.0009 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0 0.0003 0 0.0001 0.0001 0.0001 0 0 0
Nucleus 0.0117 0.0002 0.0013 0.0002 0.0017 0.0009 0.0001 0.0002 0.0001 0.0001 0.0007 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0
Peroxisomes 0.0064 0 0.0057 0 0.0005 0.0046 0.0054 0 0.0082 0 0.0016 0.0029 0.0014 0.0001 0 0 0.0001 0
Punctate Nuclear 0.004 0 0.0015 0 0.0002 0.0002 0.0004 0 0.0001 0 0.0043 0 0.0001 0 0 0 0 0
Vacuole 0.0074 0.0021 0.0038 0.003 0.0048 0.0045 0.0029 0.0028 0.0016 0.0014 0.0017 0.0023 0.0057 0.0024 0.0029 0.0037 0.0023 0.0025
Vacuole Periphery 0.0013 0.0001 0.0004 0.0001 0.0007 0.0002 0.0001 0 0 0 0.0003 0 0.0001 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0123 20.1301 23.8999 38.3712 37.4684 24.0064 26.6292 33.4274 47.0879 41.9417
Translational Efficiency 0.4781 0.3417 0.4701 0.5007 0.2939 0.5088 0.4272 0.3783 0.3168 0.3029

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
211 1051 1098 689 1888 1770 2111 1502 2099 2821 3209 2191

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 991.38 1226.71 1218.98 922.14 1159.73 1235.89 1290.48 915.01 1142.81 1232.47 1266.02 917.25
Standard Deviation 118.08 229.96 190.00 128.54 172.14 189.24 198.06 126.43 174.98 205.41 198.27 127.14
Intensity Change Log 2 — 0.307284 0.298164 -0.104452 — 0.091761 0.154119 -0.341930 — 0.195101 0.222296 -0.227612

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.046530 0.011988 0.049812 0.099577 0.000705 0.004830 0.008198 0.094560 0.005312 0.007497 0.022437 0.096138
Bud Neck 0.036817 0.007782 0.017532 0.034380 0.000911 0.003597 0.005108 0.050061 0.004520 0.005156 0.009359 0.045130
Bud Site 0.092624 0.042233 0.145068* 0.115199* 0.008041 0.020222 0.024540 0.068163 0.016543 0.028422 0.065780 0.082954
Cell Periphery 0.552109* 0.872254* 0.468104* 0.120050* 0.971716* 0.939069* 0.940892* 0.166116* 0.929536* 0.914176* 0.779121* 0.151630*
Cytoplasm 0.139339* 0.038648 0.191443* 0.219724* 0.007640 0.016199 0.007988 0.268311* 0.020879 0.024563 0.070759 0.253032*
Cytoplasmic Foci 0.013767 0.002705 0.015790 0.056490 0.000544 0.001639 0.000222 0.043288 0.001873 0.002036 0.005549 0.047440
Eisosomes 0.030363 0.004308 0.014626 0.075105 0.000617 0.002103 0.006103 0.109345* 0.003607 0.002924 0.009019 0.098577
Endoplasmic Reticulum 0.004244 0.006925 0.020582 0.017988 0.004631 0.004202 0.003121 0.008825 0.004592 0.005216 0.009095 0.011706
Endosome 0.005723 0.001921 0.005222 0.007447 0.000593 0.001142 0.000359 0.009373 0.001109 0.001432 0.002023 0.008767
Golgi 0.005050 0.002147 0.008821 0.005593 0.000275 0.001300 0.000205 0.009250 0.000755 0.001616 0.003153 0.008100
Lipid Particles 0.001364 0.000979 0.023919 0.014870 0.000351 0.000472 0.000192 0.042958 0.000453 0.000661 0.008311 0.034125
Mitochondria 0.000996 0.000725 0.001137 0.000566 0.000534 0.001068 0.000412 0.002932 0.000581 0.000940 0.000660 0.002188
Mitotic Spindle 0.000482 0.000628 0.000443 0.003685 0.000319 0.000423 0.000249 0.000627 0.000335 0.000499 0.000315 0.001588
None 0.000018 0.000238 0.000250 0.001447 0.000014 0.000173 0.000022 0.001712 0.000015 0.000198 0.000100 0.001629
Nuclear Periphery 0.000048 0.000231 0.000177 0.000223 0.000463 0.000389 0.000166 0.000297 0.000422 0.000330 0.000169 0.000274
Nuclear Periphery Foci 0.000432 0.000198 0.001175 0.002103 0.000087 0.000114 0.000102 0.000831 0.000122 0.000145 0.000469 0.001231
Nucleolus 0.000012 0.000019 0.000031 0.000095 0.000019 0.000016 0.000016 0.000345 0.000018 0.000017 0.000021 0.000266
Nucleus 0.009618 0.000211 0.004427 0.040694 0.000077 0.000163 0.000266 0.037168 0.001036 0.000181 0.001690 0.038277
Peroxisomes 0.001014 0.001399 0.004655 0.007044 0.000701 0.001028 0.000233 0.006898 0.000732 0.001166 0.001746 0.006944
Vacuole 0.059111 0.003009 0.026449 0.177222 0.001296 0.001385 0.001452 0.077716 0.007108 0.001990 0.010005 0.109007
Vacuole Periphery 0.000339 0.001454 0.000336 0.000498 0.000464 0.000467 0.000156 0.001225 0.000452 0.000835 0.000218 0.000996

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 5.00 -0.51 -5.59 -13.51 -7.09 -5.78 -6.92 -21.46 -20.24 -19.14* -2.08 -12.25 -24.84 -24.14 -19.39
Bud Neck 7.25 4.77 0.78 -8.76 -5.29 -5.57 -8.78 -21.03 -19.47 -18.83 -1.07 -7.54 -21.31 -21.02 -18.70
Bud Site 4.68 -4.57 -1.63 -8.62 3.53 -5.15 -7.24 -15.54 -11.36 -10.45 -4.87 -16.19 -17.38 -13.68 -3.69
Cell Periphery -12.32 2.83 16.31 73.12* 26.61* 7.11 7.66 121.84* 105.02* 110.15* 2.53 20.49 120.44 124.39* 82.34*
Cytoplasm 7.54 -3.48 -4.98 -19.12 -2.36 -5.10 -0.29 -44.46 -42.23 -44.40* -1.51 -14.64 -44.92 -44.63 -32.09
Cytoplasmic Foci 2.73 -0.29 -7.16 -12.78 -9.31 -2.52 2.06 -17.12 -16.49 -17.25 -0.12 -5.22 -20.68 -20.78 -18.92
Eisosomes 6.51 3.90 -6.89 -14.64 -12.36 -5.92 -10.64 -26.61 -26.20 -25.04* 1.22 -8.90 -29.38 -29.74 -27.66
Endoplasmic Reticulum -1.79 -8.70 -6.81 -5.05 1.22 0.42 1.50 -3.70 -5.08 -6.52 -0.64 -4.37 -6.47 -6.81 -2.47
Endosome 1.38 0.18 -0.53 -5.84 -2.13 -2.58 2.12 -10.59 -9.77 -10.96 -0.92 -2.71 -11.03 -11.24 -10.37
Golgi 1.27 -1.69 -0.12 -2.38 2.66 -2.03 0.80 -8.82 -6.98 -8.89 -1.84 -6.89 -8.96 -7.20 -5.85
Lipid Particles 1.11 -9.75 -8.60 -8.85 3.41 -0.42 0.80 -13.51 -13.49 -13.59 -0.77 -9.45 -15.04 -14.97 -10.85
Mitochondria 0.46 -0.23 0.77 1.07 1.68 -2.07 0.91 -2.93 -2.21 -3.12 -1.86 -0.46 -2.81 -2.14 -2.68
Mitotic Spindle -0.37 0.11 -1.87 -1.80 -1.94 -3.99 3.37 -1.38 -0.91 -1.69 -1.73 0.50 -2.29 -1.96 -2.33
None -1.02 -2.55 -1.91 -1.55 -1.59 -1.04 -3.58 -2.54 -2.24 -2.53 -1.46 -2.72 -3.14 -2.70 -2.96
Nuclear Periphery -8.61 -10.14 -2.26 0.06 -0.61 2.16 9.81 3.02 1.78 -2.77 3.11 9.35 3.06 1.26 -2.58
Nuclear Periphery Foci 3.50 -6.61 -6.77 -8.10 -3.89 -3.00 -0.91 -12.87 -12.45 -11.86 -1.64 -10.28 -12.89 -12.71 -8.42
Nucleolus -2.89 -3.92 -4.90 -4.52 -3.59 2.18 2.93 -1.99 -2.00 -2.00 0.88 -1.85 -2.20 -2.21 -2.17
Nucleus 3.46 1.79 -8.04 -14.55 -12.60 -4.50 -6.07 -19.11 -19.06 -19.00 3.06 -1.91 -23.03 -23.93 -22.67
Peroxisomes -0.98 -7.44 -6.09 -5.46 -2.16 -1.41 2.86 -11.61 -10.83 -12.93 -2.00 -4.73 -12.82 -11.67 -10.53
Vacuole 5.82 3.45 -9.22 -21.50 -18.08 -0.62 -0.73 -21.20 -21.18 -21.08 4.92 -1.84 -26.49 -29.00 -26.28
Vacuole Periphery -1.18 -0.01 -0.42 1.00 -0.46 -0.02 4.21 -1.60 -1.53 -2.29 -1.05 3.42 -1.57 -0.29 -2.31
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ammonium permease of high capacity and low affinity; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation ammonia permease activity regulated by TORC1 effectors, Npr1p and Par32p; MEP3 has a paralog, MEP1, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (99%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Mep3

Mep3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mep3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available