Standard name | |
---|---|
Human Ortholog | |
Description | Ammonium permease of high capacity and low affinity; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation ammonia permease activity regulated by TORC1 effectors, Npr1p and Par32p; MEP3 has a paralog, MEP1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 57 | 56 | 96 | 72 | 106 | 66 | 62 | 56 | 36 | 23 | 26 | 22 | 81 | 86 | 87 | 0 | 3 | 0 | 2 | 7 | 10 | 21 | 16 | 12 |
Bud | 2 | 10 | 9 | 8 | 19 | 19 | 30 | 31 | 29 | 18 | 18 | 28 | 17 | 17 | 11 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 3 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 3 | 9 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 159 | 169 | 265 | 171 | 191 | 110 | 128 | 84 | 74 | 85 | 56 | 59 | 229 | 229 | 246 | 9 | 19 | 2 | 194 | 192 | 311 | 279 | 459 | 435 |
Cytoplasm | 8 | 27 | 16 | 19 | 27 | 77 | 131 | 176 | 146 | 227 | 174 | 258 | 42 | 42 | 63 | 7 | 19 | 12 | 2 | 15 | 0 | 8 | 3 | 6 |
Endoplasmic Reticulum | 1 | 6 | 8 | 6 | 29 | 27 | 35 | 31 | 23 | 28 | 18 | 26 | 7 | 7 | 13 | 0 | 0 | 0 | 4 | 2 | 7 | 13 | 11 | 10 |
Endosome | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 7 | 3 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Mitochondria | 10 | 8 | 64 | 21 | 4 | 4 | 6 | 4 | 5 | 7 | 6 | 6 | 3 | 3 | 4 | 0 | 4 | 1 | 0 | 3 | 2 | 8 | 6 | 8 |
Nucleus | 1 | 1 | 0 | 0 | 0 | 0 | 3 | 3 | 6 | 9 | 5 | 5 | 1 | 1 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 1 | 6 | 7 | 5 | 5 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 1 | 0 | 2 | 0 | 2 | 2 | 2 | 0 | 1 | 0 | 2 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 0 | 0 | 3 | 4 | 2 | 25 | 13 | 19 | 15 | 40 | 2 | 3 | 3 | 0 | 1 | 1 | 0 | 4 | 0 | 3 | 1 | 3 |
Unique Cell Count | 175 | 202 | 308 | 211 | 265 | 210 | 288 | 306 | 251 | 318 | 248 | 350 | 280 | 279 | 322 | 16 | 45 | 16 | 208 | 236 | 339 | 348 | 508 | 501 |
Labelled Cell Count | 239 | 279 | 462 | 305 | 391 | 312 | 405 | 428 | 341 | 422 | 320 | 449 | 384 | 389 | 434 | 16 | 50 | 16 | 208 | 236 | 339 | 348 | 508 | 501 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.9 | 14.5 | 14.0 | 14.2 | 12.6 | 10.4 | 10.3 | 10.6 | 10.3 | 9.7 | 9.7 | 9.7 | 12.2 | 12.2 | 12.1 | 12.7 | 13.7 | 14.8 | 13.6 | 14.4 | 15.1 |
Std Deviation (1e-4) | 2.4 | 3.1 | 2.8 | 2.6 | 3.0 | 3.4 | 2.8 | 2.8 | 2.7 | 2.6 | 2.4 | 2.2 | 2.3 | 2.3 | 2.4 | 3.5 | 4.4 | 3.9 | 2.8 | 2.7 | 3.0 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.15 | -0.43 | -0.44 | -0.4 | -0.45 | -0.52 | -0.52 | -0.53 | -0.19 | -0.19 | -0.2 | -0.14 | -0.03 | 0.08 | -0.04 | 0.04 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.7 | 2.2 | 0.1 | -2.7 | -3.7 | -4.7 | -7.6 | -5.9 | -8.3 | -0.6 | -0.1 | -1.1 | 0 | -3.4 | 0 |
Bud | 0.5 | 2.4 | 3.0 | 3.7 | 3.6 | 4.0 | 1.7 | 2.4 | 2.8 | 1.9 | 1.9 | 0.4 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.5 | -4.1 | -8.4 | -10.7 | -14.7 | -13.6 | -14.9 | -15.1 | -17.7 | -1.4 | -1.3 | -3.1 | -3.2 | -6.9 | -7.5 |
Cytoplasm | 1.7 | 2.3 | 9.2 | 11.4 | 14.0 | 13.7 | 17.0 | 16.1 | 17.8 | 4.0 | 4.0 | 5.4 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.2 | 4.1 | 4.6 | 4.5 | 3.8 | 3.4 | 3.3 | 2.6 | 2.8 | -0.1 | -0.1 | 1.0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -3.3 | -7.1 | -6.2 | -7.1 | -7.7 | -6.7 | -7.3 | -6.5 | -7.9 | -7.5 | -7.5 | -7.9 | 0 | -1.9 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 5.1 | 4.0 | 4.4 | 4.4 | 6.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.881 | 6.9849 | 5.5839 | 5.9817 | 5.7632 | 6.0537 | 5.4681 | 7.9038 | 6.8542 | 6.4591 | 6.0181 | 7.3222 | 5.8914 | 9.0088 | 7.9068 | 8.16 | 7.0456 | 8.4511 |
Actin | 0.0226 | 0.0105 | 0.0188 | 0.0081 | 0.0139 | 0.0088 | 0.0605 | 0.0063 | 0.015 | 0.0014 | 0.017 | 0.0063 | 0.0402 | 0.0147 | 0.0105 | 0.0012 | 0.0466 | 0.0014 |
Bud | 0.0016 | 0.0005 | 0.002 | 0.0008 | 0.0012 | 0.0009 | 0.0005 | 0.0006 | 0.0005 | 0.0002 | 0.0013 | 0.0005 | 0.0006 | 0.0004 | 0.0007 | 0.0005 | 0.0005 | 0.0003 |
Bud Neck | 0.0031 | 0.0022 | 0.0065 | 0.0047 | 0.0107 | 0.0056 | 0.0037 | 0.0018 | 0.0081 | 0.0025 | 0.0087 | 0.0042 | 0.0078 | 0.0047 | 0.0121 | 0.003 | 0.0016 | 0.003 |
Bud Periphery | 0.0155 | 0.005 | 0.0114 | 0.0122 | 0.0137 | 0.0101 | 0.0065 | 0.0046 | 0.0059 | 0.0045 | 0.0216 | 0.009 | 0.006 | 0.0028 | 0.0094 | 0.0106 | 0.0143 | 0.0084 |
Bud Site | 0.0127 | 0.0265 | 0.026 | 0.0048 | 0.0049 | 0.0035 | 0.01 | 0.0308 | 0.0292 | 0.0032 | 0.0046 | 0.0021 | 0.0216 | 0.0485 | 0.0191 | 0.0024 | 0.0013 | 0.0008 |
Cell Periphery | 0.663 | 0.6772 | 0.6941 | 0.7504 | 0.7141 | 0.7807 | 0.7317 | 0.8331 | 0.7979 | 0.8732 | 0.8358 | 0.8892 | 0.7169 | 0.7763 | 0.8468 | 0.9255 | 0.8853 | 0.9179 |
Cytoplasm | 0.1069 | 0.213 | 0.1645 | 0.1738 | 0.1493 | 0.115 | 0.0577 | 0.0813 | 0.0684 | 0.0617 | 0.0464 | 0.0587 | 0.0712 | 0.1318 | 0.0849 | 0.0423 | 0.0306 | 0.04 |
Cytoplasmic Foci | 0.0089 | 0.0013 | 0.0037 | 0.0007 | 0.0012 | 0.0026 | 0.004 | 0.0004 | 0.0027 | 0.0001 | 0.0035 | 0.0013 | 0.0045 | 0.0022 | 0.0004 | 0.0001 | 0.0002 | 0.0001 |
Eisosomes | 0.0369 | 0.0377 | 0.0242 | 0.0225 | 0.0155 | 0.0297 | 0.0798 | 0.0305 | 0.0369 | 0.0432 | 0.0266 | 0.0131 | 0.0903 | 0.0038 | 0.0069 | 0.0042 | 0.0081 | 0.0195 |
Endoplasmic Reticulum | 0.0134 | 0.0153 | 0.0114 | 0.0095 | 0.0218 | 0.0121 | 0.0054 | 0.0038 | 0.0031 | 0.0069 | 0.0045 | 0.0035 | 0.0108 | 0.005 | 0.0038 | 0.0048 | 0.0073 | 0.0044 |
Endosome | 0.0157 | 0.0004 | 0.0048 | 0.0003 | 0.0063 | 0.0042 | 0.0013 | 0.0001 | 0.0006 | 0 | 0.0073 | 0.0011 | 0.0023 | 0.0003 | 0.0001 | 0 | 0 | 0 |
Golgi | 0.0093 | 0.0028 | 0.0026 | 0.0013 | 0.0038 | 0.0057 | 0.004 | 0.0006 | 0.0023 | 0.0001 | 0.0026 | 0.0026 | 0.0076 | 0.0017 | 0.0004 | 0.0002 | 0.0003 | 0.0001 |
Lipid Particles | 0.0149 | 0.0021 | 0.0048 | 0.0009 | 0.0043 | 0.0055 | 0.0058 | 0.0004 | 0.0023 | 0.0002 | 0.0037 | 0.001 | 0.0038 | 0.0044 | 0.0005 | 0.0005 | 0.0003 | 0.0006 |
Mitochondria | 0.0202 | 0.0006 | 0.003 | 0.0011 | 0.0122 | 0.0028 | 0.0036 | 0.0003 | 0.0037 | 0.0001 | 0.001 | 0.0009 | 0.003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
None | 0.0133 | 0.0019 | 0.0044 | 0.005 | 0.0165 | 0.0013 | 0.0159 | 0.0019 | 0.0129 | 0.0007 | 0.0025 | 0.001 | 0.0048 | 0.0004 | 0.0006 | 0.0004 | 0.0007 | 0.0005 |
Nuclear Periphery | 0.0091 | 0.0006 | 0.0041 | 0.0004 | 0.0024 | 0.0008 | 0.0006 | 0.0003 | 0.0003 | 0.0005 | 0.0039 | 0.0002 | 0.001 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0003 |
Nucleolus | 0.0022 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0117 | 0.0002 | 0.0013 | 0.0002 | 0.0017 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Peroxisomes | 0.0064 | 0 | 0.0057 | 0 | 0.0005 | 0.0046 | 0.0054 | 0 | 0.0082 | 0 | 0.0016 | 0.0029 | 0.0014 | 0.0001 | 0 | 0 | 0.0001 | 0 |
Punctate Nuclear | 0.004 | 0 | 0.0015 | 0 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0.0043 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0074 | 0.0021 | 0.0038 | 0.003 | 0.0048 | 0.0045 | 0.0029 | 0.0028 | 0.0016 | 0.0014 | 0.0017 | 0.0023 | 0.0057 | 0.0024 | 0.0029 | 0.0037 | 0.0023 | 0.0025 |
Vacuole Periphery | 0.0013 | 0.0001 | 0.0004 | 0.0001 | 0.0007 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.0123 | 20.1301 | 23.8999 | 38.3712 | 37.4684 | 24.0064 | 26.6292 | 33.4274 | 47.0879 | 41.9417 |
Translational Efficiency | 0.4781 | 0.3417 | 0.4701 | 0.5007 | 0.2939 | 0.5088 | 0.4272 | 0.3783 | 0.3168 | 0.3029 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
211 | 1051 | 1098 | 689 | 1888 | 1770 | 2111 | 1502 | 2099 | 2821 | 3209 | 2191 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 991.38 | 1226.71 | 1218.98 | 922.14 | 1159.73 | 1235.89 | 1290.48 | 915.01 | 1142.81 | 1232.47 | 1266.02 | 917.25 |
Standard Deviation | 118.08 | 229.96 | 190.00 | 128.54 | 172.14 | 189.24 | 198.06 | 126.43 | 174.98 | 205.41 | 198.27 | 127.14 |
Intensity Change Log 2 | — | 0.307284 | 0.298164 | -0.104452 | — | 0.091761 | 0.154119 | -0.341930 | — | 0.195101 | 0.222296 | -0.227612 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.046530 | 0.011988 | 0.049812 | 0.099577 | 0.000705 | 0.004830 | 0.008198 | 0.094560 | 0.005312 | 0.007497 | 0.022437 | 0.096138 |
Bud Neck | 0.036817 | 0.007782 | 0.017532 | 0.034380 | 0.000911 | 0.003597 | 0.005108 | 0.050061 | 0.004520 | 0.005156 | 0.009359 | 0.045130 |
Bud Site | 0.092624 | 0.042233 | 0.145068* | 0.115199* | 0.008041 | 0.020222 | 0.024540 | 0.068163 | 0.016543 | 0.028422 | 0.065780 | 0.082954 |
Cell Periphery | 0.552109* | 0.872254* | 0.468104* | 0.120050* | 0.971716* | 0.939069* | 0.940892* | 0.166116* | 0.929536* | 0.914176* | 0.779121* | 0.151630* |
Cytoplasm | 0.139339* | 0.038648 | 0.191443* | 0.219724* | 0.007640 | 0.016199 | 0.007988 | 0.268311* | 0.020879 | 0.024563 | 0.070759 | 0.253032* |
Cytoplasmic Foci | 0.013767 | 0.002705 | 0.015790 | 0.056490 | 0.000544 | 0.001639 | 0.000222 | 0.043288 | 0.001873 | 0.002036 | 0.005549 | 0.047440 |
Eisosomes | 0.030363 | 0.004308 | 0.014626 | 0.075105 | 0.000617 | 0.002103 | 0.006103 | 0.109345* | 0.003607 | 0.002924 | 0.009019 | 0.098577 |
Endoplasmic Reticulum | 0.004244 | 0.006925 | 0.020582 | 0.017988 | 0.004631 | 0.004202 | 0.003121 | 0.008825 | 0.004592 | 0.005216 | 0.009095 | 0.011706 |
Endosome | 0.005723 | 0.001921 | 0.005222 | 0.007447 | 0.000593 | 0.001142 | 0.000359 | 0.009373 | 0.001109 | 0.001432 | 0.002023 | 0.008767 |
Golgi | 0.005050 | 0.002147 | 0.008821 | 0.005593 | 0.000275 | 0.001300 | 0.000205 | 0.009250 | 0.000755 | 0.001616 | 0.003153 | 0.008100 |
Lipid Particles | 0.001364 | 0.000979 | 0.023919 | 0.014870 | 0.000351 | 0.000472 | 0.000192 | 0.042958 | 0.000453 | 0.000661 | 0.008311 | 0.034125 |
Mitochondria | 0.000996 | 0.000725 | 0.001137 | 0.000566 | 0.000534 | 0.001068 | 0.000412 | 0.002932 | 0.000581 | 0.000940 | 0.000660 | 0.002188 |
Mitotic Spindle | 0.000482 | 0.000628 | 0.000443 | 0.003685 | 0.000319 | 0.000423 | 0.000249 | 0.000627 | 0.000335 | 0.000499 | 0.000315 | 0.001588 |
None | 0.000018 | 0.000238 | 0.000250 | 0.001447 | 0.000014 | 0.000173 | 0.000022 | 0.001712 | 0.000015 | 0.000198 | 0.000100 | 0.001629 |
Nuclear Periphery | 0.000048 | 0.000231 | 0.000177 | 0.000223 | 0.000463 | 0.000389 | 0.000166 | 0.000297 | 0.000422 | 0.000330 | 0.000169 | 0.000274 |
Nuclear Periphery Foci | 0.000432 | 0.000198 | 0.001175 | 0.002103 | 0.000087 | 0.000114 | 0.000102 | 0.000831 | 0.000122 | 0.000145 | 0.000469 | 0.001231 |
Nucleolus | 0.000012 | 0.000019 | 0.000031 | 0.000095 | 0.000019 | 0.000016 | 0.000016 | 0.000345 | 0.000018 | 0.000017 | 0.000021 | 0.000266 |
Nucleus | 0.009618 | 0.000211 | 0.004427 | 0.040694 | 0.000077 | 0.000163 | 0.000266 | 0.037168 | 0.001036 | 0.000181 | 0.001690 | 0.038277 |
Peroxisomes | 0.001014 | 0.001399 | 0.004655 | 0.007044 | 0.000701 | 0.001028 | 0.000233 | 0.006898 | 0.000732 | 0.001166 | 0.001746 | 0.006944 |
Vacuole | 0.059111 | 0.003009 | 0.026449 | 0.177222 | 0.001296 | 0.001385 | 0.001452 | 0.077716 | 0.007108 | 0.001990 | 0.010005 | 0.109007 |
Vacuole Periphery | 0.000339 | 0.001454 | 0.000336 | 0.000498 | 0.000464 | 0.000467 | 0.000156 | 0.001225 | 0.000452 | 0.000835 | 0.000218 | 0.000996 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 5.00 | -0.51 | -5.59 | -13.51 | -7.09 | -5.78 | -6.92 | -21.46 | -20.24 | -19.14* | -2.08 | -12.25 | -24.84 | -24.14 | -19.39 |
Bud Neck | 7.25 | 4.77 | 0.78 | -8.76 | -5.29 | -5.57 | -8.78 | -21.03 | -19.47 | -18.83 | -1.07 | -7.54 | -21.31 | -21.02 | -18.70 |
Bud Site | 4.68 | -4.57 | -1.63 | -8.62 | 3.53 | -5.15 | -7.24 | -15.54 | -11.36 | -10.45 | -4.87 | -16.19 | -17.38 | -13.68 | -3.69 |
Cell Periphery | -12.32 | 2.83 | 16.31 | 73.12* | 26.61* | 7.11 | 7.66 | 121.84* | 105.02* | 110.15* | 2.53 | 20.49 | 120.44 | 124.39* | 82.34* |
Cytoplasm | 7.54 | -3.48 | -4.98 | -19.12 | -2.36 | -5.10 | -0.29 | -44.46 | -42.23 | -44.40* | -1.51 | -14.64 | -44.92 | -44.63 | -32.09 |
Cytoplasmic Foci | 2.73 | -0.29 | -7.16 | -12.78 | -9.31 | -2.52 | 2.06 | -17.12 | -16.49 | -17.25 | -0.12 | -5.22 | -20.68 | -20.78 | -18.92 |
Eisosomes | 6.51 | 3.90 | -6.89 | -14.64 | -12.36 | -5.92 | -10.64 | -26.61 | -26.20 | -25.04* | 1.22 | -8.90 | -29.38 | -29.74 | -27.66 |
Endoplasmic Reticulum | -1.79 | -8.70 | -6.81 | -5.05 | 1.22 | 0.42 | 1.50 | -3.70 | -5.08 | -6.52 | -0.64 | -4.37 | -6.47 | -6.81 | -2.47 |
Endosome | 1.38 | 0.18 | -0.53 | -5.84 | -2.13 | -2.58 | 2.12 | -10.59 | -9.77 | -10.96 | -0.92 | -2.71 | -11.03 | -11.24 | -10.37 |
Golgi | 1.27 | -1.69 | -0.12 | -2.38 | 2.66 | -2.03 | 0.80 | -8.82 | -6.98 | -8.89 | -1.84 | -6.89 | -8.96 | -7.20 | -5.85 |
Lipid Particles | 1.11 | -9.75 | -8.60 | -8.85 | 3.41 | -0.42 | 0.80 | -13.51 | -13.49 | -13.59 | -0.77 | -9.45 | -15.04 | -14.97 | -10.85 |
Mitochondria | 0.46 | -0.23 | 0.77 | 1.07 | 1.68 | -2.07 | 0.91 | -2.93 | -2.21 | -3.12 | -1.86 | -0.46 | -2.81 | -2.14 | -2.68 |
Mitotic Spindle | -0.37 | 0.11 | -1.87 | -1.80 | -1.94 | -3.99 | 3.37 | -1.38 | -0.91 | -1.69 | -1.73 | 0.50 | -2.29 | -1.96 | -2.33 |
None | -1.02 | -2.55 | -1.91 | -1.55 | -1.59 | -1.04 | -3.58 | -2.54 | -2.24 | -2.53 | -1.46 | -2.72 | -3.14 | -2.70 | -2.96 |
Nuclear Periphery | -8.61 | -10.14 | -2.26 | 0.06 | -0.61 | 2.16 | 9.81 | 3.02 | 1.78 | -2.77 | 3.11 | 9.35 | 3.06 | 1.26 | -2.58 |
Nuclear Periphery Foci | 3.50 | -6.61 | -6.77 | -8.10 | -3.89 | -3.00 | -0.91 | -12.87 | -12.45 | -11.86 | -1.64 | -10.28 | -12.89 | -12.71 | -8.42 |
Nucleolus | -2.89 | -3.92 | -4.90 | -4.52 | -3.59 | 2.18 | 2.93 | -1.99 | -2.00 | -2.00 | 0.88 | -1.85 | -2.20 | -2.21 | -2.17 |
Nucleus | 3.46 | 1.79 | -8.04 | -14.55 | -12.60 | -4.50 | -6.07 | -19.11 | -19.06 | -19.00 | 3.06 | -1.91 | -23.03 | -23.93 | -22.67 |
Peroxisomes | -0.98 | -7.44 | -6.09 | -5.46 | -2.16 | -1.41 | 2.86 | -11.61 | -10.83 | -12.93 | -2.00 | -4.73 | -12.82 | -11.67 | -10.53 |
Vacuole | 5.82 | 3.45 | -9.22 | -21.50 | -18.08 | -0.62 | -0.73 | -21.20 | -21.18 | -21.08 | 4.92 | -1.84 | -26.49 | -29.00 | -26.28 |
Vacuole Periphery | -1.18 | -0.01 | -0.42 | 1.00 | -0.46 | -0.02 | 4.21 | -1.60 | -1.53 | -2.29 | -1.05 | 3.42 | -1.57 | -0.29 | -2.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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