Standard name | |
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Human Ortholog | |
Description | Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 5 | 3 | 3 | 4 |
Bud | 2 | 0 | 2 | 1 | 2 | 3 | 12 | 6 | 7 | 10 | 9 | 5 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 5 | 1 | 1 | 2 | 5 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 2 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 2 | 3 | 3 | 0 | 0 | 1 | 0 | 4 | 2 | 0 | 3 | 2 | 4 | 1 | 0 | 1 | 1 | 2 | 7 | 2 | 1 | 2 |
Cytoplasm | 96 | 182 | 304 | 196 | 239 | 271 | 302 | 286 | 218 | 153 | 143 | 147 | 153 | 204 | 202 | 48 | 67 | 16 | 59 | 133 | 188 | 47 | 92 | 108 |
Endoplasmic Reticulum | 7 | 9 | 2 | 5 | 14 | 1 | 11 | 1 | 7 | 6 | 1 | 14 | 24 | 13 | 5 | 3 | 5 | 2 | 54 | 31 | 71 | 38 | 32 | 32 |
Endosome | 0 | 3 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 8 | 5 | 11 | 2 | 4 | 4 | 1 | 1 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 1 |
Mitochondria | 57 | 4 | 23 | 23 | 47 | 158 | 270 | 292 | 287 | 273 | 251 | 288 | 1 | 0 | 0 | 3 | 12 | 1 | 38 | 35 | 62 | 25 | 28 | 41 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 4 | 1 | 1 | 4 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 3 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 3 | 3 | 4 | 1 | 3 | 0 | 2 | 0 | 0 | 2 | 2 | 1 | 1 | 2 | 0 | 1 | 3 |
Vac/Vac Membrane | 3 | 3 | 1 | 1 | 7 | 7 | 12 | 11 | 9 | 7 | 9 | 10 | 5 | 6 | 11 | 4 | 11 | 3 | 6 | 8 | 6 | 7 | 21 | 24 |
Unique Cell Count | 146 | 198 | 325 | 214 | 285 | 359 | 508 | 509 | 435 | 381 | 346 | 380 | 185 | 223 | 222 | 63 | 98 | 24 | 184 | 231 | 359 | 132 | 197 | 236 |
Labelled Cell Count | 168 | 201 | 341 | 230 | 315 | 446 | 615 | 607 | 543 | 467 | 429 | 474 | 192 | 230 | 233 | 65 | 110 | 32 | 184 | 231 | 359 | 132 | 197 | 236 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 4.4 | 3.4 | 3.5 | 3.5 | 3.3 | 2.8 | 3.0 | 2.8 | 2.6 | 2.8 | 2.8 | 5.8 | 5.3 | 6.7 | 5.6 | 5.5 | 6.2 | 6.0 | 6.2 | 6.4 |
Std Deviation (1e-4) | 1.5 | 0.9 | 0.9 | 1.0 | 0.8 | 0.9 | 0.5 | 0.7 | 0.7 | 0.4 | 0.7 | 0.6 | 1.0 | 1.0 | 13.8 | 0.9 | 1.0 | 1.6 | 1.1 | 1.4 | 1.6 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.02 | -0.04 | -0.27 | -0.21 | -0.29 | -0.38 | -0.31 | -0.27 | 0.76 | 0.62 | 0.95 | 0.71 | 0.68 | 0.86 | 0.8 | 0.84 | 0.89 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4883 | 2.4871 | 2.2358 | 2.2246 | 2.4363 | 2.2646 | 4.3753 | 4.5245 | 4.5984 | 4.0679 | 3.979 | 4.4852 | 2.199 | 2.6435 | 1.9703 | 1.8551 | 2.0389 | 1.9534 |
Actin | 0.0294 | 0.0005 | 0.0129 | 0.0081 | 0.0159 | 0.0056 | 0.0068 | 0.0009 | 0.0025 | 0.0016 | 0.0011 | 0.0035 | 0.0005 | 0.0004 | 0.001 | 0.0005 | 0.0015 | 0.0028 |
Bud | 0.001 | 0.0001 | 0.0032 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Bud Neck | 0.0055 | 0.0004 | 0.0049 | 0.0007 | 0.0011 | 0.001 | 0.0007 | 0.0003 | 0.0006 | 0.0011 | 0.0005 | 0.0015 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0209 | 0.0015 |
Bud Periphery | 0.0016 | 0.0001 | 0.0017 | 0.0011 | 0.001 | 0.0004 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0131 | 0.0039 | 0.0085 | 0.0013 | 0.0018 | 0.0003 | 0.0018 | 0.0015 | 0.0009 | 0.0002 | 0.0001 | 0.0003 | 0.0012 | 0.0004 | 0.0009 | 0.0001 | 0.0011 | 0.0002 |
Cell Periphery | 0.0018 | 0.001 | 0.0011 | 0.0007 | 0.0005 | 0.0005 | 0.0012 | 0.0008 | 0.0011 | 0.0018 | 0.0006 | 0.0008 | 0.0008 | 0.0006 | 0.0006 | 0.0006 | 0.0005 | 0.0005 |
Cytoplasm | 0.0609 | 0.1866 | 0.124 | 0.0784 | 0.1667 | 0.1439 | 0.0514 | 0.0807 | 0.0887 | 0.0464 | 0.1277 | 0.0901 | 0.0645 | 0.109 | 0.0726 | 0.0943 | 0.0938 | 0.0913 |
Cytoplasmic Foci | 0.0485 | 0.0439 | 0.0513 | 0.0703 | 0.0337 | 0.0285 | 0.0319 | 0.0528 | 0.0501 | 0.0426 | 0.0243 | 0.0378 | 0.0451 | 0.0406 | 0.0511 | 0.0282 | 0.0446 | 0.0442 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.3794 | 0.3558 | 0.4075 | 0.3787 | 0.2336 | 0.3992 | 0.4605 | 0.3713 | 0.4 | 0.6044 | 0.5267 | 0.469 | 0.3136 | 0.4112 | 0.3551 | 0.4917 | 0.3903 | 0.382 |
Endosome | 0.1491 | 0.1476 | 0.1411 | 0.1712 | 0.1494 | 0.1197 | 0.1187 | 0.1741 | 0.1614 | 0.099 | 0.1264 | 0.1132 | 0.1876 | 0.1763 | 0.1596 | 0.1607 | 0.1543 | 0.1537 |
Golgi | 0.0153 | 0.0112 | 0.0145 | 0.0267 | 0.0135 | 0.0102 | 0.0113 | 0.017 | 0.0161 | 0.0117 | 0.0095 | 0.0099 | 0.0105 | 0.0131 | 0.0144 | 0.023 | 0.0117 | 0.0109 |
Lipid Particles | 0.1513 | 0.1164 | 0.1006 | 0.0733 | 0.0648 | 0.1299 | 0.2041 | 0.1873 | 0.176 | 0.0899 | 0.0627 | 0.1761 | 0.2491 | 0.1553 | 0.2319 | 0.102 | 0.1215 | 0.1834 |
Mitochondria | 0.0124 | 0.0019 | 0.0088 | 0.048 | 0.0162 | 0.0109 | 0.0038 | 0.0013 | 0.0035 | 0.0092 | 0.0019 | 0.0076 | 0.0031 | 0.0035 | 0.0037 | 0.0045 | 0.0058 | 0.0045 |
None | 0.0232 | 0.0302 | 0.0257 | 0.0188 | 0.1582 | 0.0443 | 0.039 | 0.0496 | 0.0377 | 0.0046 | 0.0512 | 0.0234 | 0.0151 | 0.0185 | 0.033 | 0.0147 | 0.042 | 0.0246 |
Nuclear Periphery | 0.0434 | 0.0572 | 0.0506 | 0.0451 | 0.0475 | 0.0649 | 0.038 | 0.0358 | 0.0309 | 0.0477 | 0.0405 | 0.0362 | 0.0651 | 0.0435 | 0.0483 | 0.053 | 0.0786 | 0.0605 |
Nucleolus | 0.0075 | 0.0008 | 0.0006 | 0.0009 | 0.0049 | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0015 | 0.0002 | 0.0003 | 0.0003 | 0.0006 | 0.0008 |
Nucleus | 0.0093 | 0.0065 | 0.0034 | 0.0135 | 0.0152 | 0.0082 | 0.0027 | 0.0019 | 0.0027 | 0.0031 | 0.0042 | 0.0045 | 0.0054 | 0.0033 | 0.003 | 0.004 | 0.0084 | 0.0107 |
Peroxisomes | 0.0055 | 0.0015 | 0.0107 | 0.0188 | 0.04 | 0.0018 | 0.0022 | 0.0011 | 0.0036 | 0.0045 | 0.0008 | 0.0033 | 0.0018 | 0.001 | 0.003 | 0.001 | 0.0064 | 0.0027 |
Punctate Nuclear | 0.0098 | 0.0073 | 0.0103 | 0.0271 | 0.0187 | 0.0077 | 0.0066 | 0.0069 | 0.0043 | 0.0011 | 0.0034 | 0.0059 | 0.0091 | 0.0026 | 0.0036 | 0.0066 | 0.0043 | 0.0076 |
Vacuole | 0.0271 | 0.0235 | 0.0148 | 0.0116 | 0.0122 | 0.0177 | 0.0157 | 0.0142 | 0.0167 | 0.0257 | 0.0161 | 0.0132 | 0.0214 | 0.0168 | 0.0138 | 0.0113 | 0.0104 | 0.0145 |
Vacuole Periphery | 0.0044 | 0.0037 | 0.0036 | 0.0046 | 0.0039 | 0.0044 | 0.0024 | 0.0019 | 0.0024 | 0.0051 | 0.0018 | 0.003 | 0.0037 | 0.0033 | 0.0034 | 0.003 | 0.003 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.3786 | 32.77 | 25.1273 | 41.0028 | 43.5679 | 36.6377 | 31.477 | 32.0536 | 26.2649 | 49.1777 |
Translational Efficiency | 1.1777 | 0.9936 | 1.2447 | 0.7847 | 0.9128 | 1.1762 | 1.0008 | 0.7932 | 1.0357 | 0.9031 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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