Standard name
Human Ortholog
Description Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.16 0.18
Bud 0 0 0 0 0 0 0 – 0.05 0 0.1 0 0 0 0 0 0 0 0 0 0 0.12 0.1 0.12
Bud Neck 0 0 0 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.06 0.05 0.08 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.11 0.2 0.2 0.14 0.13 0.18 – 0.16 0.17 0.14 0.2 0.22 0.36 0.38 0.14 0.11 0.12 0 0.05 0 0.07 0.1 0.11
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Endosome 0.1 0.17 0 0.05 0 0.08 0 – 0 0 0 0 0.2 0.24 0.18 0.18 0.28 0.16 0.09 0.08 0.09 0.14 0.14 0.16
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.17 0 0.08 0.07 0.23 0.34 – 0.54 0.57 0.49 0.39 0.12 0.05 0 0.15 0.07 0.07 0 0 0.05 0.12 0.07 0
Nucleus 0.27 0.25 0.17 0.19 0.11 0.22 0.2 – 0.17 0.2 0.15 0.14 0.05 0.05 0.05 0.15 0.14 0.19 0.19 0.22 0.12 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.33 0.22 0.14 0.15 0.19 0.14 0.15 – 0.11 0.13 0.18 0.11 0.31 0.36 0.35 0.39 0.38 0.4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
SpindlePole 0.34 0.27 0.5 0.54 0.62 0.4 0.34 – 0.24 0.22 0.29 0.31 0.26 0.22 0.2 0.39 0.45 0.46 0.51 0.42 0.5 0.24 0.21 0.22
Vac/Vac Membrane 0.1 0.18 0.1 0 0 0 0 – 0 0.06 0 0 0.11 0.07 0.06 0.1 0.1 0.09 0.06 0.07 0.08 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 2 2 2 3 1 – 0 1 0 1 1 0 0 0 0 0 0 1 1 21 36 42
Bud 0 2 4 7 12 16 21 – 20 15 33 13 1 0 0 4 1 5 12 9 10 24 22 28
Bud Neck 0 0 12 1 19 6 16 – 9 10 11 12 0 0 0 1 0 2 1 2 4 5 8 9
Bud Site 3 2 2 8 12 11 19 – 23 17 25 19 4 0 1 2 0 2 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 27 31 62 52 52 57 86 – 60 60 47 75 54 103 99 41 22 24 12 15 18 14 23 24
Endoplasmic Reticulum 0 1 2 0 1 0 2 – 1 0 0 1 0 0 0 12 7 4 0 0 0 5 10 5
Endosome 35 45 13 12 9 32 10 – 7 3 3 17 49 68 47 55 55 32 41 28 37 27 32 37
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 3 0 4 4
Mitochondria 66 45 12 22 25 97 159 – 201 208 161 147 28 13 8 44 13 14 7 11 19 23 15 6
Nucleus 98 68 54 48 41 92 94 – 65 71 50 53 13 13 14 45 27 37 83 75 51 2 0 0
Nuclear Periphery 3 2 0 0 0 0 0 – 1 0 1 0 2 0 1 1 1 2 1 1 1 0 0 0
Nucleolus 120 60 44 39 69 59 70 – 42 46 58 43 75 102 93 117 75 80 14 15 11 0 0 0
Peroxisomes 1 5 1 0 1 4 0 – 5 0 1 2 4 8 10 0 2 3 4 1 3 9 8 7
SpindlePole 121 74 159 141 231 171 161 – 89 81 94 118 63 63 53 118 88 91 228 143 204 48 48 52
Vac/Vac Membrane 35 49 31 5 16 16 15 – 10 23 11 16 27 20 17 29 19 18 25 22 33 10 6 2
Unique Cell Count 361 272 317 259 370 423 468 374 363 329 378 242 284 262 301 196 198 449 339 413 202 227 233
Labelled Cell Count 510 384 399 337 490 564 654 533 535 495 517 321 390 343 469 310 314 449 339 413 202 227 233


Microtubule, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 4.9 5.4 4.9 5.5 4.5 4.5 – 3.9 3.9 3.6 4.2 4.5 4.9 4.7 7.2 7.7 7.0 6.3 7.7 9.4
Std Deviation (1e-4) 0.7 0.9 0.9 1.3 1.3 1.1 1.3 – 1.0 1.0 0.8 1.1 0.8 1.6 1.2 1.6 1.6 1.5 1.9 2.2 2.4
Intensity Change (Log2) – – – -0.13 0.03 -0.27 -0.25 – -0.46 -0.47 -0.56 -0.35 -0.24 -0.14 -0.21 0.43 0.51 0.38 0.23 0.52 0.81


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -0.1 0 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.374 0.4391 0.444 0.6338 0.3171 0.135 1.1807 1.1668 1.3609 1.3667 0.7456 0.903 0.7104 0.6997 0.8574 1.0332 1.4826 0.2944
Actin 0.0007 0.0003 0.0001 0.0002 0.0001 0.0005 0.011 0.0001 0.0052 0.009 0.0017 0.0007 0.0357 0.0002 0.0062 0.0001 0.0348 0.0037
Bud 0.0001 0.0003 0.0001 0 0 0.0002 0.0003 0.0002 0.0002 0.0001 0 0.0001 0.0003 0.0004 0.0002 0 0.0004 0.0001
Bud Neck 0.0013 0.0032 0.0076 0.0047 0.0008 0.0062 0.003 0.0011 0.003 0.0235 0.0011 0.0061 0.0014 0.0025 0.0095 0.0012 0.0015 0.012
Bud Periphery 0.0001 0.0002 0.0001 0 0 0.0002 0.0006 0.0001 0.0002 0.0002 0.0002 0.0001 0.0006 0.0002 0.0003 0 0.0013 0.0001
Bud Site 0.0013 0.0034 0.0007 0.0003 0.0001 0.0002 0.0054 0.004 0.0009 0.0006 0.0002 0.0002 0.0009 0.0121 0.0075 0.0004 0.0004 0.0005
Cell Periphery 0.0001 0.0001 0 0 0 0 0.0002 0 0 0.0001 0 0 0.0001 0.0001 0.0007 0 0.0001 0
Cytoplasm 0.0347 0.0497 0.0049 0.0012 0.0002 0.0147 0.0303 0.0231 0.0021 0.0003 0.0005 0.0127 0.0372 0.0397 0.0131 0.0002 0.0019 0.0221
Cytoplasmic Foci 0.0267 0.0061 0.0126 0.0119 0.02 0.0073 0.0242 0.0024 0.0089 0.014 0.0035 0.0035 0.0629 0.0162 0.0157 0.0257 0.0175 0.0036
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0.0001 0.0009 0.0001 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0022 0.0026 0.0002 0.0001 0 0.0009 0.0041 0.0012 0.0004 0.0001 0.0003 0.0003 0.0049 0.0009 0.0027 0 0.0045 0.0006
Endosome 0.0054 0.0029 0.0013 0.0025 0.0015 0.0019 0.0135 0.0012 0.0042 0.002 0.0029 0.0021 0.0237 0.0014 0.0032 0.0008 0.0213 0.0027
Golgi 0.0007 0.0002 0.0003 0.0004 0.0011 0.0002 0.0032 0.0001 0.002 0.0018 0.0024 0.0003 0.0075 0.0002 0.0009 0.0005 0.0172 0.0005
Lipid Particles 0.0089 0.0018 0.0033 0.0042 0.0161 0.0036 0.0228 0.0004 0.0095 0.0124 0.0201 0.0013 0.017 0.0012 0.0027 0.0037 0.0209 0.0012
Mitochondria 0.0006 0.0004 0.0001 0.0001 0 0.0002 0.0045 0.0002 0.0009 0.0139 0.0017 0.0002 0.0023 0.0004 0.0004 0 0.0044 0.0002
None 0.0839 0.073 0.0037 0.0008 0.0002 0.0327 0.055 0.0298 0.006 0.0003 0.0006 0.0144 0.1713 0.1003 0.0081 0.0002 0.0014 0.0507
Nuclear Periphery 0.0181 0.0179 0.0055 0.0021 0.0018 0.007 0.0395 0.0116 0.0055 0.0048 0.0334 0.009 0.0332 0.0086 0.0042 0.002 0.048 0.0071
Nucleolus 0.0218 0.0477 0.0358 0.0311 0.0294 0.031 0.019 0.0506 0.0401 0.011 0.04 0.0353 0.0056 0.0405 0.0733 0.0333 0.0243 0.0393
Nucleus 0.2523 0.3694 0.0981 0.0789 0.0139 0.3882 0.1876 0.2769 0.0517 0.006 0.0266 0.3251 0.1326 0.3181 0.0729 0.038 0.0146 0.4084
Peroxisomes 0.0031 0.0002 0.0005 0.0005 0.0019 0.001 0.0071 0.0001 0.0058 0.0152 0.0016 0.0008 0.0069 0.0004 0.0006 0.0006 0.0019 0.0007
Punctate Nuclear 0.5363 0.4176 0.8246 0.8606 0.9128 0.5035 0.5654 0.5961 0.853 0.8833 0.8618 0.5873 0.4529 0.4555 0.7767 0.8931 0.7808 0.4461
Vacuole 0.0012 0.0027 0.0005 0.0002 0.0001 0.0004 0.0017 0.0007 0.0003 0.0002 0.0004 0.0003 0.0017 0.0008 0.0007 0.0001 0.0009 0.0003
Vacuole Periphery 0.0004 0.0005 0.0001 0.0001 0 0.0001 0.0013 0.0002 0.0002 0.0002 0.001 0.0001 0.0012 0.0004 0.0002 0 0.0018 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.6036 13.0616 0.6684 4.6171 12.4117 6.0427 13.3204 7.9915 6.177 15.0808
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
327 691 103 943 1619 1102 1795 2542 1946 1793 1898 3485

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 628.80 723.38 948.77 908.45 675.18 711.21 839.93 920.70 667.39 715.90 845.84 917.39
Standard Deviation 70.58 127.64 135.48 125.90 74.21 90.10 126.24 133.77 75.63 106.31 129.14 131.80
Intensity Change Log 2 — 0.202152 0.593457 0.530806 — 0.075003 0.314997 0.447459 — 0.137717 0.455990 0.488252

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000181 0.000617 0.001508 0.000720 0.000459 0.000587 0.000195 0.000316 0.000412 0.000598 0.000267 0.000425
Bud Neck 0.010672 0.008619 0.002744 0.002678 0.007392 0.010918 0.004870 0.003234 0.007943 0.010032 0.004754 0.003084
Bud Site 0.003723 0.003950 0.022816 0.007740 0.004096 0.007923 0.002502 0.006336 0.004033 0.006392 0.003604 0.006716
Cell Periphery 0.000259 0.000224 0.000182 0.000046 0.000341 0.000254 0.000264 0.000040 0.000327 0.000242 0.000260 0.000041
Cytoplasm 0.166173* 0.081992 0.014943 0.011073 0.156127* 0.122402* 0.056457 0.005727 0.157815* 0.106828* 0.054204 0.007174
Cytoplasmic Foci 0.119066 0.054064 0.006444 0.009240 0.086032 0.092495 0.009648 0.001602 0.091583 0.077684 0.009474 0.003669
Eisosomes 0.000111 0.000093 0.000107 0.000007 0.000096 0.000094 0.000055 0.000006 0.000099 0.000094 0.000058 0.000007
Endoplasmic Reticulum 0.001121 0.002663 0.009508 0.000597 0.002375 0.001853 0.000836 0.000315 0.002164 0.002165 0.001307 0.000391
Endosome 0.004667 0.007181 0.002409 0.005356 0.006257 0.009920 0.002620 0.001153 0.005989 0.008865 0.002609 0.002290
Golgi 0.001301 0.003289 0.000531 0.004862 0.002900 0.005974 0.001120 0.000351 0.002632 0.004939 0.001088 0.001572
Lipid Particles 0.013530 0.010901 0.003925 0.000776 0.019730 0.019572 0.001652 0.000311 0.018688 0.016230 0.001775 0.000437
Mitochondria 0.008528 0.006343 0.000600 0.001480 0.005768 0.012260 0.001616 0.000748 0.006232 0.009979 0.001561 0.000946
Mitotic Spindle 0.069919 0.284088* 0.456248* 0.913928* 0.114150* 0.202070* 0.573056* 0.951377* 0.106717* 0.233679* 0.566717* 0.941244*
None 0.026187 0.024356 0.002602 0.001546 0.038662 0.020385 0.025643 0.000866 0.036566 0.021916 0.024392 0.001050
Nuclear Periphery 0.001175 0.001666 0.001074 0.000576 0.003392 0.001692 0.000637 0.000356 0.003019 0.001682 0.000660 0.000416
Nuclear Periphery Foci 0.027617 0.035655 0.019007 0.006945 0.044107 0.030971 0.007919 0.005927 0.041336 0.032777 0.008521 0.006202
Nucleolus 0.094026 0.085790 0.017977 0.003776 0.105946 0.072591 0.034833 0.006725 0.103943 0.077678 0.033918 0.005927
Nucleus 0.439276* 0.371319* 0.406607* 0.012333 0.380697* 0.359552* 0.271550* 0.009962 0.390541* 0.364087* 0.278879* 0.010603
Peroxisomes 0.008120 0.011124 0.002634 0.000868 0.014436 0.021417 0.001563 0.001368 0.013374 0.017450 0.001621 0.001233
Vacuole 0.004021 0.005393 0.027903 0.007398 0.006576 0.006286 0.002718 0.002785 0.006147 0.005942 0.004085 0.004033
Vacuole Periphery 0.000326 0.000672 0.000231 0.008054 0.000462 0.000782 0.000247 0.000493 0.000439 0.000740 0.000246 0.002539

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.19 -4.57 -4.36 -1.27 3.22 -2.82 -1.08 -0.39 1.13 0.08 -5.34 -4.19 -2.28 0.45 0.18
Bud Neck -0.34 0.57 0.73 1.25 0.04 -2.76 -1.42 0.54 3.04 1.79 -3.17 -0.89 0.51 3.45 1.28
Bud Site -2.53 -1.81 -3.95 -2.40 0.90 -2.27 -2.16 -4.99 -2.40 -3.02 -3.25 -2.85 -6.29 -3.31 -2.93
Cell Periphery 2.10 0.23 7.69 7.29 5.71 3.45 5.32 14.38 10.39 8.53 4.39 5.34 15.86 12.86 8.96
Cytoplasm 4.70 8.61 9.45 7.85 0.77 2.84 13.81 21.40 16.10 9.75 5.57 15.42 23.16 17.91 9.19
Cytoplasmic Foci 7.55 11.21 11.89 10.05 0.74 2.13 21.52 24.36 19.64 6.88 6.75 24.17 26.72 22.13 5.72
Eisosomes 1.57 -1.28 12.94 15.04 6.36 0.44 10.34 30.94 22.12 15.07 0.84 9.99 32.97 27.01 15.71
Endoplasmic Reticulum -6.48 -6.27 -2.83 2.27 5.79 0.66 0.28 7.23 7.84 7.80 -2.26 -3.41 4.75 7.76 7.53
Endosome -1.58 1.52 3.22 4.27 1.41 -3.08 7.86 12.81 9.87 3.47 -3.46 8.35 11.42 11.07 2.12
Golgi -1.71 2.83 0.48 1.42 -0.88 -1.78 8.74 15.36 7.85 2.30 -1.48 8.71 10.39 7.91 0.58
Lipid Particles 2.51 4.81 7.53 8.07 4.43 0.06 13.71 15.18 11.40 6.09 1.82 15.03 16.81 13.83 6.52
Mitochondria 0.33 1.66 1.51 2.67 -1.03 -3.06 5.40 6.94 6.06 3.46 -2.23 4.76 5.63 6.65 2.58
Mitotic Spindle -10.44 -6.71 -44.04* -29.68* -9.64 -6.64 -30.63* -80.59* -53.65* -27.51* -10.84 -32.58 -89.63 -60.88* -28.16*
None 2.30 8.86 9.13 7.35 0.54 5.60 6.76 18.84 14.04 10.06 6.29 7.30 20.55 15.38 9.83
Nuclear Periphery -2.03 -1.95 0.92 2.76 2.42 2.74 3.78 5.38 7.20 5.60 2.54 3.64 5.29 7.89 4.98
Nuclear Periphery Foci 1.10 1.07 6.23 7.99 3.20 3.82 11.62 17.16 11.20 7.20 4.36 11.67 18.16 13.91 7.66
Nucleolus 0.79 6.43 10.60 15.01 2.44 4.50 9.71 23.58* 16.13 14.63 4.07 10.65 26.06 21.88 15.33
Nucleus 0.94 -1.18 12.08 15.82 7.56 -0.39 3.01 25.89 21.30 21.69 -0.46 3.22 28.24 26.88 22.41
Peroxisomes -1.07 2.86 7.62 8.42 3.44 -2.95 12.18 13.23 12.80 2.81 -2.39 13.06 14.95 15.16 3.98
Vacuole -2.86 -4.29 -4.47 -3.63 3.30 -0.82 -0.81 -1.99 -1.29 -1.36 -1.32 -3.28 -4.05 -3.29 -0.71
Vacuole Periphery -2.10 -2.42 -2.45 -2.27 -2.35 -2.40 2.81 -0.02 1.49 -1.01 -3.00 2.53 -2.38 -1.86 -2.60
Standard name
Human Ortholog
Description Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate
Localization
Cell Percentages spindle pole (33%), nucleus (27%), mixed (17%)
Cell Cycle Regulation Yes
spindle pole - S/G2 (1.2e-06)
spindle pole - MA (1.4e-06)
nucleus - T/G1 (9.6e-11)
Subcompartmental Group N/A

Kar3

Kar3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kar3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available