Standard name
Human Ortholog
Description Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.07 0.32 0.32 0.14 0 0 0 0 0 0 0 0.05 0 0 0.06 0.56 0.55 0.62 0 0 0 0 0 0
Cytoplasm 0.99 0.8 0.76 0.94 0.97 0.98 0.97 0.95 0.97 0.96 0.96 0.91 0.98 0.98 0.99 0.54 0.56 0.54 0.98 0.93 0.92 0.95 0.97 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 3 1 0 1 2 4 0 0 1 3 0 1 1 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 52 69 35 11 6 10 6 3 4 5 15 6 12 14 94 80 106 0 10 11 1 0 2
Cytoplasm 133 131 161 235 284 384 416 349 197 268 263 293 185 270 248 90 81 92 133 243 306 191 170 214
Endoplasmic Reticulum 1 0 1 0 0 1 4 9 5 10 7 14 0 0 0 5 6 2 0 2 6 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 1 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 1 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 0 0 1 0 0 0 0 2 2 3 0 0 0 0 0 0 1 1 0 0
Unique Cell Count 135 164 213 249 292 391 427 367 204 280 274 321 189 275 251 167 145 171 137 263 335 202 176 228
Labelled Cell Count 144 184 235 273 297 393 434 370 205 282 277 329 194 286 263 189 168 200 137 263 335 202 176 228


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 493.3 565.5 536.9 471.2 406.1 340.5 299.4 273.5 246.7 254.5 225.9 249.6 438.8 437.0 441.9 646.0 661.6 653.3 476.9 484.1 489.1
Std Deviation (1e-4) 85.1 110.7 87.0 82.2 84.5 71.0 67.9 63.3 68.9 64.4 67.1 64.7 126.9 94.4 98.0 118.6 135.0 117.1 92.6 105.2 127.3
Intensity Change (Log2) – – – -0.19 -0.4 -0.66 -0.84 -0.97 -1.12 -1.08 -1.25 -1.11 -0.29 -0.3 -0.28 0.27 0.3 0.28 -0.17 -0.15 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -4.7 -8.7 -11.0 -10.9 -10.6 -8.4 -9.6 -9.3 -8.6 -7.5 -8.3 -7.5 4.7 4.3 5.8
Cytoplasm 5.8 7.4 8.9 8.7 7.0 6.1 6.6 6.7 5.0 6.4 7.7 7.7 -4.4 -3.9 -4.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 2.7 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 491.8856 582.0804 518.7791 495.8317 473.0639 522.4252 378.5588 465.4692 378.2098 394.0977 392.483 445.1563 441.5915 566.7711 505.0848 382.2734 428.7634 554.6757
Actin 0.0012 0.0002 0.0002 0.0003 0.0003 0.0004 0.0008 0.0001 0.0014 0.0003 0.0002 0.0003 0.002 0.0001 0.0051 0.0004 0.0005 0.0002
Bud 0.001 0.0002 0.0006 0.0007 0.0014 0.0001 0.0013 0.0003 0.0004 0.0004 0.0002 0.0002 0.0029 0 0.0014 0.0004 0.0008 0.0002
Bud Neck 0.0004 0.0003 0.0004 0.0006 0.0009 0.0017 0.0001 0.0001 0.0003 0.0003 0.0003 0.0008 0.0003 0.0002 0.0007 0.0003 0.0004 0.0006
Bud Periphery 0.0006 0 0.0002 0.0001 0.0007 0 0.0005 0.0001 0.0006 0.0001 0 0 0.0014 0 0.0006 0.0001 0.0005 0.0001
Bud Site 0.0002 0.0001 0.0006 0.0001 0.0003 0 0.0003 0.0002 0.0002 0.0001 0 0 0.001 0 0.0013 0.0003 0.0005 0
Cell Periphery 0.0034 0.0029 0.0011 0.0015 0.0017 0.0005 0.0011 0.0002 0.0005 0.0011 0 0.0001 0.0057 0.0019 0.0015 0.0008 0.0057 0.0005
Cytoplasm 0.9041 0.9775 0.9403 0.8926 0.7869 0.9696 0.8972 0.9773 0.8837 0.8671 0.9828 0.9735 0.852 0.9899 0.8767 0.7936 0.7893 0.9598
Cytoplasmic Foci 0.0013 0.0001 0.0033 0.0002 0.0102 0.0003 0.0032 0.0011 0.0037 0.0086 0.0004 0.0003 0.009 0 0.0211 0.0701 0.0199 0.0002
Eisosomes 0.0003 0 0.0005 0 0.0005 0 0.0007 0.0001 0.0001 0.0003 0 0 0.0009 0 0.001 0.0008 0.0015 0
Endoplasmic Reticulum 0.0022 0.0004 0.0002 0.0001 0.0002 0.0004 0.0005 0.0001 0.0059 0.0006 0.0001 0.0007 0.0016 0.0001 0.0006 0.0031 0.0004 0.0001
Endosome 0.002 0 0 0 0.0002 0.0001 0.0002 0 0.0037 0.0005 0 0.0001 0.0064 0 0.0009 0.0202 0.0004 0
Golgi 0.0002 0 0 0 0 0 0.0001 0 0.0004 0.0001 0 0 0.0033 0 0.0004 0.0009 0.0002 0
Lipid Particles 0.0005 0.0001 0.0004 0 0.0009 0.0001 0.0009 0.0001 0.0003 0.003 0 0 0.0018 0 0.0021 0.0026 0.0057 0.0001
Mitochondria 0.0041 0.0003 0.0034 0.0024 0.0348 0.0003 0.0105 0.0043 0.0313 0.0029 0.0002 0.0004 0.0103 0.0001 0.0145 0.0302 0.0261 0.001
None 0.0058 0.0003 0.0074 0.0007 0.0221 0.0004 0.0154 0.0019 0.0043 0.0129 0.0004 0.0005 0.0158 0.0002 0.0229 0.0233 0.0244 0.0007
Nuclear Periphery 0.0187 0.0045 0.0024 0.0113 0.0085 0.0068 0.0174 0.0009 0.0249 0.0099 0.0026 0.006 0.0139 0.0023 0.0054 0.0073 0.0092 0.0042
Nucleolus 0.0136 0.0002 0.0205 0.0054 0.0377 0.0003 0.011 0.0021 0.0051 0.0156 0 0.0001 0.0235 0.0001 0.0252 0.0174 0.0377 0.0091
Nucleus 0.0323 0.0108 0.0131 0.0818 0.0764 0.0166 0.0338 0.01 0.0263 0.0613 0.0119 0.0163 0.036 0.0048 0.0117 0.0198 0.0261 0.0216
Peroxisomes 0.0002 0 0.0002 0.0001 0.0009 0 0.0004 0.0001 0.0002 0.0003 0 0 0.0006 0 0.0009 0.0012 0.0016 0
Punctate Nuclear 0.0005 0 0.0003 0.0001 0.0006 0.0002 0.0004 0.0001 0.0007 0.0019 0.0002 0.0002 0.002 0 0.0018 0.0013 0.0009 0.0002
Vacuole 0.005 0.0017 0.0033 0.0011 0.0073 0.0016 0.0023 0.0006 0.0026 0.0057 0.0003 0.0004 0.0068 0.0004 0.0022 0.0039 0.0272 0.0009
Vacuole Periphery 0.0024 0.0002 0.0019 0.0009 0.0076 0.0005 0.0018 0.0004 0.0032 0.0068 0.0002 0.0002 0.0029 0 0.0019 0.0019 0.0209 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 630.0125 686.8483 688.9289 693.0242 753.1727 542.0946 647.7674 581.8882 635.2805 692.4835
Translational Efficiency 0.8628 0.9359 0.9008 1.0432 0.9909 0.9621 1.0283 1.023 1.0197 1.0597

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1747 1404 366 764 1617 1818 214 1261 3364 3222 580 2025

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 22555.87 33840.86 36332.47 9343.07 30562.85 32410.15 25278.78 9353.78 26404.65 33033.59 32254.04 9349.74
Standard Deviation 5249.01 9014.63 5694.80 3768.75 6534.41 7885.41 4659.37 3242.04 7129.99 8426.08 7544.75 3450.22
Intensity Change Log 2 — 0.585263 0.687757 -1.271534 — 0.084667 -0.273852 -1.708158 — 0.318722 0.213973 -1.506425

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000063 0.000084 0.000141 0.000660 0.000046 0.000063 0.000111 0.000283 0.000055 0.000072 0.000130 0.000425
Bud Neck 0.000131 0.000400 0.003699 0.004060 0.000212 0.000478 0.002658 0.002799 0.000170 0.000444 0.003315 0.003275
Bud Site 0.000376 0.001059 0.000909 0.012452 0.001157 0.000602 0.001162 0.009731 0.000751 0.000801 0.001002 0.010757
Cell Periphery 0.001915 0.024064 0.038986 0.011274 0.008286 0.003661 0.022567 0.004551 0.004977 0.012551 0.032928 0.007088
Cytoplasm 0.910802* 0.832048* 0.584544* 0.732077* 0.904314* 0.884296* 0.652044* 0.797849* 0.907683* 0.861529* 0.609449* 0.773034*
Cytoplasmic Foci 0.002292 0.002280 0.024592 0.012057 0.002081 0.002516 0.021427 0.006914 0.002191 0.002413 0.023424 0.008854
Eisosomes 0.000000 0.000002 0.000011 0.000157 0.000001 0.000001 0.000009 0.000005 0.000001 0.000002 0.000010 0.000062
Endoplasmic Reticulum 0.000016 0.000124 0.000042 0.000080 0.000244 0.000043 0.000021 0.000119 0.000125 0.000079 0.000034 0.000104
Endosome 0.000024 0.000041 0.000088 0.001396 0.000045 0.000038 0.000075 0.001451 0.000034 0.000039 0.000083 0.001430
Golgi 0.002623 0.002570 0.005598 0.001486 0.002817 0.001557 0.002753 0.001305 0.002716 0.001998 0.004548 0.001373
Lipid Particles 0.000775 0.006408 0.012323 0.011978 0.002387 0.002154 0.010725 0.003685 0.001550 0.004008 0.011733 0.006814
Mitochondria 0.000075 0.000172 0.000190 0.000590 0.000119 0.000161 0.000117 0.001053 0.000096 0.000166 0.000163 0.000879
Mitotic Spindle 0.000334 0.000068 0.000237 0.003802 0.000115 0.000151 0.000328 0.006000 0.000229 0.000115 0.000271 0.005171
None 0.011476 0.008594 0.005083 0.014412 0.006708 0.008791 0.004263 0.014620 0.009184 0.008705 0.004781 0.014542
Nuclear Periphery 0.000111 0.000305 0.002586 0.000364 0.000148 0.000152 0.001617 0.000206 0.000128 0.000219 0.002228 0.000265
Nuclear Periphery Foci 0.001199 0.002203 0.004019 0.002343 0.001321 0.001263 0.002669 0.001139 0.001258 0.001673 0.003521 0.001593
Nucleolus 0.000030 0.000069 0.000097 0.000583 0.000051 0.000066 0.000082 0.000999 0.000040 0.000067 0.000091 0.000842
Nucleus 0.061166 0.040842 0.192342* 0.093230 0.038107 0.076243 0.179736* 0.073266 0.050082 0.060817 0.187691* 0.080798
Peroxisomes 0.000004 0.000021 0.000005 0.000299 0.000012 0.000004 0.000006 0.000611 0.000008 0.000011 0.000006 0.000494
Vacuole 0.004501 0.076476 0.117670 0.095197 0.025635 0.016317 0.095832 0.072533 0.014659 0.042531 0.109612 0.081084
Vacuole Periphery 0.002086 0.002170 0.006839 0.001504 0.006195 0.001443 0.001798 0.000881 0.004061 0.001760 0.004979 0.001116

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.18 -1.78 -3.45 -3.93 -2.88 -1.06 -6.48 -6.99 -4.34 -2.55 -0.27 -3.87 -5.48 -5.75 -3.62
Bud Neck -3.87 -10.65 -8.85 -8.11 0.51 -2.38 -8.59 -8.89 -7.19 0.61 -3.63 -13.57 -12.42 -10.61 1.14
Bud Site -2.32 -3.77 -5.77 -5.27 -5.36 1.66 -1.53 -7.45 -7.93 -6.95 -0.67 -3.00 -9.56 -9.39 -9.01
Cell Periphery -12.20 -10.95 -4.75 8.29 9.04 4.52 -4.51 4.87 0.32 6.60 -7.72 -11.30 0.45 8.20 11.47
Cytoplasm 12.46 24.98 25.62 14.88 -7.16 5.75 16.59 22.74 18.71 -7.13 13.04 29.72 34.37 23.12 -11.50
Cytoplasmic Foci -0.12 -9.51 -5.41 -5.45 5.48 -2.23 -9.50 -8.10 -6.71 7.36 -1.65 -13.25 -9.15 -8.53 9.28
Eisosomes -6.37 -11.34 -1.17 -1.13 -0.91 -0.69 -8.14 -8.05 -6.70 5.07 -4.75 -13.89 -1.31 -1.25 -0.65
Endoplasmic Reticulum -3.23 -4.27 -5.13 1.83 -1.13 1.64 1.80 1.41 -1.43 -2.62 0.83 1.47 1.00 0.53 -2.23
Endosome -1.76 -6.83 -1.93 -1.88 -1.70 -0.33 -3.37 -2.09 -2.07 -1.89 -1.44 -7.08 -2.80 -2.75 -2.51
Golgi 0.12 -4.36 4.18 5.38 8.05 4.17 0.38 6.03 2.60 4.76 2.75 -3.98 6.91 5.48 9.14
Lipid Particles -9.20 -10.51 -5.83 -1.57 2.63 -0.12 -5.89 -4.80 -3.73 4.18 -5.83 -11.73 -7.94 -3.18 5.86
Mitochondria -2.02 -9.80 -3.26 -1.89 -1.69 -1.40 -0.80 -2.02 -1.77 -1.95 -2.57 -7.42 -2.81 -2.22 -2.16
Mitotic Spindle 1.33 0.67 -2.20 -2.57 -2.42 0.56 -1.02 -2.84 -2.96 -2.62 1.38 0.05 -3.54 -3.89 -3.61
None 2.58 7.85 2.08 0.28 -2.14 -1.76 3.98 -0.85 0.17 -2.85 1.09 8.16 0.99 0.30 -3.37
Nuclear Periphery -7.35 -11.78 -7.52 -2.02 10.44 -1.10 -8.40 -6.64 -5.74 7.62 -6.25 -14.22 -10.40 -4.91 12.90
Nuclear Periphery Foci -2.33 -5.84 -2.54 -0.64 3.92 -0.09 -5.27 -1.97 -2.11 4.41 -1.86 -7.79 -2.72 -1.34 6.56
Nucleolus -3.79 -9.91 -4.35 -3.91 -3.47 -2.07 -4.45 -2.16 -2.06 -1.97 -4.14 -9.90 -3.09 -2.88 -2.67
Nucleus 5.92 -15.81 -11.31 -13.92 7.33 -12.98 -15.52 -15.06 -3.27 9.18 -6.98 -22.01 -17.83 -12.00 12.39
Peroxisomes -2.97 -1.56 -1.97 -1.73 -1.94 2.64 0.93 -1.72 -1.77 -1.74 -1.38 0.23 -2.18 -2.14 -2.19
Vacuole -17.34 -12.99 -19.97 -8.15 -0.82 2.26 -7.84 -17.93 -20.06 0.04 -12.60 -14.75 -27.71 -18.87 -0.06
Vacuole Periphery -0.31 -4.61 2.30 4.01 6.11 4.79 4.50 5.79 2.25 3.68 4.27 -0.85 6.34 4.37 6.89
Standard name
Human Ortholog
Description Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Asn1

Asn1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Asn1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available