ORF
Human Ortholog
Description Bifunctional triacylglycerol lipase and short chain ester hydrolase; null mutant results in the accumulation of both triacylglycerol and fatty acids derived from neutral lipids and phospholipids as well as an increase in the quantity of lipid droplets; contains an alpha/beta hydrolase domain with a conserved GXSXG lipase motif; localizes to lipid droplets; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0.1 0.07 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.96 0.97 0.98 0.97 0.96 – 0.85 0.74 0.6 0.6 0.33 0.49 0.73 0.95 0.99 – – 0.86 0.78 0.79 0.74 0.84 0.79 0.74
Endoplasmic Reticulum 0 0 0 0 0 – 0 0.1 0 0.06 0 0 0 0 0 – – 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0.16 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0.09 – 0.28 0.1 0.53 0.46 0.67 0.6 0 0 0 – – 0 0.1 0.09 0.14 0.06 0.06 0.09
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0.07 0 0 0 0.09 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 – 0 2 0 1 0 0 1 1 0 – – 0 2 11 2 2 4 6
Bud 1 2 0 0 1 – 0 1 1 1 2 2 2 0 0 – – 0 0 2 0 1 9 6
Bud Neck 0 3 1 1 2 – 0 4 3 1 2 2 3 3 0 – – 2 1 3 1 2 2 1
Bud Site 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 – – 0 – – – – – –
Cell Periphery 0 3 8 0 0 – 0 0 1 0 0 1 2 0 1 – – 3 1 3 3 1 2 2
Cytoplasm 160 484 123 39 54 – 34 31 26 21 27 48 233 405 491 – – 121 149 389 103 226 219 252
Endoplasmic Reticulum 4 7 2 0 0 – 0 4 0 2 3 3 7 9 0 – – 13 3 2 2 1 1 1
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 52 9 4 – – 5 3 8 1 3 3 8
Golgi 1 0 0 0 0 – 0 0 0 0 0 0 1 1 0 – – 0 1 1 1 0 0 2
Mitochondria 3 15 0 2 5 – 11 4 23 16 54 58 2 0 0 – – 6 18 46 19 16 16 29
Nucleus 0 3 0 0 2 – 1 0 0 1 0 2 1 1 1 – – 4 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 2 1 – – 0 0 1 0 1 1 1
Nucleolus 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 1 0 0 0 – 0 0 0 0 0 0 4 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 3 0 1 2 28 4 0 – – 2 0 6 1 2 3 9
Vac/Vac Membrane 0 3 0 0 0 – 0 0 0 0 0 1 6 2 4 – – 8 5 5 0 4 5 7
Unique Cell Count 167 500 126 40 56 40 42 43 35 81 97 318 425 497 141 191 495 139 271 278 342
Labelled Cell Count 169 522 134 43 64 46 46 57 43 89 120 342 437 502 164 191 495 139 271 278 342


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.5 4.8 4.5 3.4 – 3.4 3.8 3.1 3.2 3.1 3.3 5.1 4.6 4.4 4.2 4.9 5.1 5.1 5.6 5.3
Std Deviation (1e-4) 0.6 0.7 0.9 1.5 0.6 – 0.6 0.6 1.1 0.5 0.4 0.7 1.4 1.0 1.5 1.3 1.2 1.2 1.7 2.2 1.6
Intensity Change (Log2) – – – -0.1 -0.49 – -0.5 -0.34 -0.63 -0.6 -0.63 -0.55 0.1 -0.07 -0.13 -0.21 0.02 0.09 0.08 0.22 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.344 3.0502 3.2168 3.0039 2.9818 3.5183 4.9072 3.9496 4.032 3.4878 4.0263 3.9995 1.1328 1.6447 1.3761 1.3235 1.3182 1.4503
Actin 0.0227 0.0006 0.0125 0.0045 0.0239 0.0086 0.0505 0.0003 0.0045 0.0042 0.0155 0.0029 0.0375 0.0004 0.0043 0.0003 0.0004 0.0012
Bud 0.0012 0.0041 0.0049 0.0017 0.0003 0.0001 0.0015 0.0001 0.0011 0.0052 0.0007 0.0001 0.0003 0.0015 0.0004 0 0.0044 0.0028
Bud Neck 0.0018 0.0002 0.0015 0.0004 0.0009 0.0018 0.0049 0.0001 0.0004 0.001 0.0003 0.0016 0.004 0.0002 0.0007 0.0001 0.0007 0.0007
Bud Periphery 0.0029 0.0012 0.0044 0.0094 0.0004 0.0004 0.0022 0.0001 0.0023 0.0113 0.0013 0.0002 0.0005 0.0005 0.0005 0 0.0022 0.0021
Bud Site 0.005 0.014 0.0091 0.0007 0.0038 0.0002 0.0061 0.0013 0.0032 0.0009 0.0013 0.0004 0.0056 0.0084 0.0064 0.0001 0.0012 0.0002
Cell Periphery 0.0007 0.0004 0.0009 0.0004 0.0003 0.0001 0.0005 0.0002 0.0003 0.0003 0.0001 0.0001 0.0004 0.0003 0.0003 0 0.0002 0.0001
Cytoplasm 0.0998 0.1772 0.126 0.1136 0.2468 0.196 0.0969 0.1336 0.1041 0.2286 0.2224 0.2496 0.0636 0.1282 0.1154 0.1511 0.1157 0.1596
Cytoplasmic Foci 0.0665 0.0988 0.0639 0.0496 0.0548 0.0682 0.0636 0.043 0.066 0.1006 0.0485 0.0586 0.0584 0.0238 0.0389 0.0673 0.0093 0.0269
Eisosomes 0.0008 0.0001 0.0008 0.0004 0.0003 0.0002 0.0007 0.0002 0.0003 0.0002 0.0001 0.0002 0.0005 0.0002 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0053 0.0113 0.0043 0.0073 0.0135 0.0039 0.0066 0.0051 0.0068 0.0071 0.0059 0.005 0.007 0.0044 0.0068 0.0044 0.0017 0.0014
Endosome 0.0262 0.0422 0.0342 0.0292 0.0592 0.0404 0.0698 0.0715 0.044 0.0629 0.0949 0.0343 0.0244 0.0132 0.0166 0.0112 0.004 0.011
Golgi 0.0099 0.0026 0.0054 0.009 0.0071 0.0087 0.0175 0.0019 0.007 0.0044 0.0114 0.0067 0.0079 0.0008 0.0013 0.0012 0.0001 0.0021
Lipid Particles 0.0272 0.0181 0.0155 0.0391 0.0121 0.0227 0.0396 0.0213 0.0225 0.0155 0.0408 0.0185 0.0163 0.011 0.0114 0.0397 0.0003 0.006
Mitochondria 0.0216 0.0008 0.019 0.0053 0.0111 0.0031 0.0162 0.0006 0.0066 0.0037 0.014 0.0017 0.0045 0.0004 0.0011 0.0003 0.0004 0.005
None 0.6433 0.6145 0.6765 0.6988 0.5283 0.6135 0.5651 0.6968 0.7055 0.5296 0.4722 0.6075 0.7018 0.7946 0.7827 0.7117 0.8504 0.7672
Nuclear Periphery 0.007 0.0014 0.0013 0.0043 0.0049 0.0073 0.0079 0.0084 0.004 0.0024 0.0042 0.0022 0.0082 0.0014 0.0015 0.001 0.0006 0.0006
Nucleolus 0.0011 0.0004 0.0007 0.0005 0.0002 0.0006 0.0015 0.0004 0.0005 0.0005 0.0006 0.0002 0.002 0.0005 0.0002 0.0001 0.0006 0.0002
Nucleus 0.002 0.0022 0.002 0.0011 0.0012 0.0028 0.0163 0.0074 0.0019 0.002 0.002 0.0009 0.0147 0.0033 0.001 0.0005 0.0034 0.0009
Peroxisomes 0.041 0.0028 0.0109 0.0201 0.0212 0.0061 0.0138 0.0015 0.0051 0.0076 0.0534 0.0042 0.0298 0.0012 0.0046 0.0084 0.0001 0.004
Punctate Nuclear 0.0104 0.0039 0.002 0.002 0.001 0.0124 0.0104 0.0018 0.004 0.0063 0.0021 0.0029 0.0088 0.0037 0.0009 0.0014 0.0033 0.0068
Vacuole 0.0027 0.0029 0.0036 0.0018 0.0064 0.0021 0.0064 0.0038 0.0087 0.0046 0.0059 0.0015 0.0028 0.0017 0.0039 0.0008 0.0008 0.0009
Vacuole Periphery 0.0008 0.0004 0.0004 0.0008 0.0021 0.0007 0.0019 0.0006 0.0013 0.0011 0.0023 0.0006 0.0008 0.0002 0.0007 0.0001 0.0002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.0886 19.4041 29.5973 29.5768 15.6351 16.8733 29.9742 26.4837 25.9205 32.3125
Translational Efficiency 1.5088 1.1938 0.9211 0.8475 1.5887 2.047 1.0877 1.4177 1.1152 1.0139

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2348 528 1537 777 2468 2291 2338 1629 4816 2819 3875 2406

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 707.18 684.49 966.45 1036.73 668.64 804.58 947.97 989.16 687.43 782.09 955.30 1004.52
Standard Deviation 96.97 137.50 130.37 157.63 79.50 91.40 123.62 176.01 90.52 111.92 126.66 171.74
Intensity Change Log 2 — -0.047048 0.450618 0.551891 — 0.267006 0.503612 0.564974 — 0.114120 0.476616 0.558264

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000181 0.002145 0.038266 0.029209 0.000134 0.000681 0.008356 0.027550 0.000157 0.000956 0.020220 0.028086
Bud Neck 0.019065 0.020387 0.004204 0.004733 0.013816 0.032384 0.001992 0.011748 0.016375 0.030137 0.002870 0.009483
Bud Site 0.004548 0.009844 0.010902 0.018193 0.004318 0.013102 0.004697 0.017995 0.004430 0.012492 0.007159 0.018059
Cell Periphery 0.000134 0.000164 0.000205 0.000110 0.000149 0.000209 0.000059 0.000342 0.000141 0.000201 0.000117 0.000267
Cytoplasm 0.451942* 0.276672* 0.039876 0.006948 0.510709* 0.257502* 0.004182 0.024011 0.482057* 0.261093* 0.018339 0.018501
Cytoplasmic Foci 0.222722* 0.316886* 0.168454 0.286500* 0.187234* 0.361330* 0.322757* 0.274899* 0.204536* 0.353005* 0.261553* 0.278645*
Eisosomes 0.000263 0.000212 0.000149 0.000086 0.000187 0.000380 0.000047 0.000364 0.000224 0.000349 0.000088 0.000274
Endoplasmic Reticulum 0.001425 0.003305 0.028696 0.002115 0.001681 0.001897 0.000624 0.001697 0.001556 0.002160 0.011759 0.001832
Endosome 0.011550 0.055034 0.111563 0.208630* 0.008222 0.052931 0.247633* 0.202797* 0.009844 0.053325 0.193661 0.204681*
Golgi 0.003269 0.015915 0.030017 0.092637 0.002772 0.026038 0.054532 0.095686 0.003014 0.024142 0.044809 0.094701
Lipid Particles 0.018343 0.033382 0.338315* 0.099788 0.011315 0.055565 0.166698* 0.146350* 0.014741 0.051410 0.234769* 0.131313*
Mitochondria 0.005647 0.012992 0.001464 0.007479 0.004803 0.023766 0.002632 0.012244 0.005215 0.021748 0.002169 0.010705
Mitotic Spindle 0.002967 0.009884 0.077356 0.161944* 0.002164 0.009563 0.092848 0.075259 0.002556 0.009623 0.086703 0.103253*
None 0.004054 0.007115 0.000372 0.000071 0.004297 0.002662 0.000111 0.004128 0.004179 0.003496 0.000215 0.002817
Nuclear Periphery 0.000644 0.001068 0.002744 0.001985 0.000424 0.000656 0.000427 0.000816 0.000531 0.000733 0.001346 0.001193
Nuclear Periphery Foci 0.000428 0.003580 0.078192 0.016681 0.000371 0.000761 0.013456 0.010127 0.000399 0.001289 0.039133 0.012244
Nucleolus 0.001970 0.007333 0.000610 0.001194 0.001332 0.002161 0.000690 0.004711 0.001643 0.003129 0.000658 0.003575
Nucleus 0.197462* 0.097968 0.011608 0.000840 0.207759* 0.068071 0.000381 0.013464 0.202739* 0.073671 0.004834 0.009387
Peroxisomes 0.007303 0.038408 0.032485 0.033240 0.003783 0.034915 0.068899 0.052352 0.005499 0.035569 0.054455 0.046180
Vacuole 0.045353 0.084840 0.024113 0.024856 0.033782 0.052872 0.008407 0.021391 0.039423 0.058860 0.014637 0.022510
Vacuole Periphery 0.000732 0.002863 0.000410 0.002762 0.000749 0.002554 0.000572 0.002070 0.000741 0.002612 0.000507 0.002293

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.02 -11.69 -7.30 -6.70 1.95 -7.36 -8.26 -10.39 -10.18 -6.69 -5.92 -13.92 -12.70 -12.30 -2.82
Bud Neck -0.44 11.79 9.78 5.85 -1.84 -10.92 15.51 1.27 9.90 -6.92 -9.27 18.91 5.42 12.25 -6.93
Bud Site -4.72 -5.62 -7.27 -5.04 -4.30 -6.88 -0.85 -8.32 -3.27 -8.08 -7.90 -4.73 -11.01 -5.04 -8.64
Cell Periphery -2.20 -3.75 1.74 3.03 4.27 -2.11 11.90 -3.49 -2.15 -5.14 -2.54 2.06 -3.40 -1.57 -3.85
Cytoplasm 13.79 66.91* 79.73* 22.36 8.56 34.92* 100.06* 87.52* 39.89 -9.09 36.80 119.09* 114.85* 46.73 -0.82
Cytoplasmic Foci -8.40 9.18 -4.67 3.73 -10.67 -27.77* -21.95 -12.55 11.85 6.26 -28.04 -11.83 -12.13 12.80 -2.21
Eisosomes 2.69 7.00 11.64 8.15 7.11 -8.21 18.29 -1.33 0.20 -2.44 -6.16 15.77 -0.50 0.92 -2.10
Endoplasmic Reticulum -3.69 -11.58 -1.79 1.85 11.16 -0.62 3.28 -0.13 0.59 -4.58 -2.45 -10.55 -1.23 1.10 10.20
Endosome -9.73 -27.69* -26.92* -17.65 -11.36 -20.54 -51.67* -35.80* -25.31 7.27 -22.34 -55.28* -44.50* -31.14 -0.76
Golgi -4.57 -14.70 -17.80 -13.35 -11.53 -15.23 -30.74* -26.61 -17.99 -10.15 -15.82 -33.24* -32.01 -21.88 -15.42
Lipid Particles -3.96 -40.09* -12.12 -8.65 24.12* -16.88* -31.40* -23.03 -14.10 3.03 -16.35 -48.34 -25.59 -15.62 16.76
Mitochondria -3.47 6.80 -0.84 2.22 -3.46 -11.31 2.73 -4.10 5.46 -5.71 -12.02 5.83 -4.03 6.51 -6.73
Mitotic Spindle -2.99 -15.82 -16.61 -15.61 -8.74 -5.05 -21.53* -15.45 -13.69 2.32 -5.74 -26.67 -22.24 -20.39 -4.06
None -1.43 6.12 6.74 3.44 3.42 2.70 9.78 -0.34 -1.77 -4.36 1.09 10.83 1.29 0.38 -4.16
Nuclear Periphery -3.07 -7.29 -2.44 -1.71 0.99 -3.41 -0.79 -3.09 -1.41 -2.66 -3.18 -6.93 -3.41 -2.42 0.41
Nuclear Periphery Foci -4.20 -18.85 -6.40 -4.82 12.32 -4.26 -10.23 -8.38 -8.04 2.12 -5.56 -20.71 -10.42 -9.51 11.89
Nucleolus -3.37 3.14 1.32 3.73 -1.15 -2.34 1.17 -4.61 -3.62 -5.11 -3.62 2.96 -3.84 -1.04 -5.27
Nucleus 12.61 45.46 51.87* 13.93 7.44 32.65 57.56* 48.12* 18.45 -7.12 37.23 73.83* 66.97* 24.46 -2.89
Peroxisomes -7.73 -10.13 -8.20 1.06 -0.12 -17.32 -22.48* -17.09 -5.21 4.17 -18.21 -24.10 -18.56 -3.88 2.90
Vacuole -6.87 6.91 0.92 6.47 -2.87 -7.97 14.20 2.50 8.49 -7.09 -8.95 15.45 3.35 9.74 -6.51
Vacuole Periphery -5.28 5.11 -3.18 0.16 -3.73 -10.06 1.43 -5.23 1.89 -5.66 -11.67 2.96 -5.85 1.30 -6.66
ORF
Human Ortholog
Description Bifunctional triacylglycerol lipase and short chain ester hydrolase; null mutant results in the accumulation of both triacylglycerol and fatty acids derived from neutral lipids and phospholipids as well as an increase in the quantity of lipid droplets; contains an alpha/beta hydrolase domain with a conserved GXSXG lipase motif; localizes to lipid droplets; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS
Localization
Cell Percentages cytoplasm (23%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YPR147C

YPR147C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypr147c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available