Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); NCE102 has a paralog, FHN1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 57 | 33 | 76 | 113 | 126 | 105 | 137 | 102 | 113 | 157 | 126 | 133 | 93 | 111 | 99 | 64 | 60 | 28 | 102 | 88 | 87 | 49 | 125 | 96 |
Bud | 10 | 7 | 19 | 35 | 56 | 50 | 44 | 42 | 45 | 66 | 41 | 43 | 12 | 4 | 11 | 8 | 8 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 69 | 53 | 48 | 107 | 124 | 83 | 113 | 64 | 96 | 96 | 95 | 99 | 80 | 139 | 137 | 22 | 18 | 15 | 71 | 66 | 42 | 47 | 96 | 138 |
Cytoplasm | 5 | 7 | 2 | 5 | 18 | 10 | 20 | 22 | 26 | 34 | 25 | 40 | 3 | 4 | 4 | 10 | 12 | 21 | 0 | 1 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 11 | 16 | 11 | 18 | 26 | 33 | 63 | 66 | 35 | 72 | 49 | 71 | 27 | 38 | 44 | 9 | 7 | 11 | 10 | 8 | 13 | 5 | 8 | 19 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Golgi | 64 | 36 | 31 | 51 | 46 | 22 | 47 | 55 | 43 | 131 | 52 | 52 | 52 | 74 | 60 | 91 | 70 | 52 | 2 | 4 | 3 | 5 | 12 | 6 |
Mitochondria | 13 | 1 | 23 | 14 | 55 | 58 | 57 | 42 | 49 | 38 | 78 | 70 | 2 | 3 | 0 | 28 | 18 | 11 | 8 | 8 | 3 | 10 | 17 | 13 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 4 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 3 | 0 | 3 | 2 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 6 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Unique Cell Count | 142 | 106 | 126 | 205 | 283 | 227 | 326 | 277 | 255 | 386 | 296 | 333 | 162 | 235 | 239 | 155 | 145 | 113 | 198 | 180 | 154 | 123 | 266 | 280 |
Labelled Cell Count | 230 | 153 | 214 | 343 | 456 | 363 | 487 | 397 | 408 | 596 | 470 | 511 | 269 | 373 | 355 | 234 | 199 | 145 | 198 | 180 | 154 | 123 | 266 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 50.9 | 62.1 | 45.4 | 39.8 | 33.0 | 31.8 | 33.0 | 35.2 | 35.5 | 38.3 | 40.0 | 42.5 | 44.5 | 49.2 | 58.7 | 78.8 | 81.3 | 72.3 | 48.6 | 50.9 | 44.0 |
Std Deviation (1e-4) | 12.8 | 16.8 | 16.4 | 9.1 | 9.1 | 9.1 | 10.2 | 10.6 | 13.3 | 12.5 | 16.4 | 16.8 | 12.9 | 16.3 | 21.4 | 29.6 | 26.6 | 30.1 | 13.7 | 13.0 | 10.1 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.46 | -0.51 | -0.46 | -0.37 | -0.35 | -0.25 | -0.18 | -0.09 | -0.03 | 0.11 | 0.37 | 0.79 | 0.84 | 0.67 | 0.1 | 0.16 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.9 | -2.9 | -2.5 | -3.5 | -4.4 | -2.9 | -3.8 | -3.3 | -3.9 | -0.5 | -2.4 | -3.4 | -3.2 | -3.1 | -5.5 |
Bud | 0.5 | 1.1 | 1.6 | -0.4 | 0 | 0.6 | 0.5 | -0.3 | -0.6 | -2.1 | -5.0 | -3.5 | -2.8 | -2.6 | -2.5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.5 | 1.1 | -0.3 | -0.7 | -3.1 | -0.1 | -2.9 | -1.2 | -1.7 | 1.9 | 3.8 | 3.5 | -4.6 | -4.9 | -4.3 |
Cytoplasm | 0 | 2.1 | 0 | 2.0 | 2.5 | 3.0 | 2.8 | 2.6 | 3.5 | 0 | 0 | 0 | 2.0 | 2.5 | 4.4 |
Endoplasmic Reticulum | 0 | 0.1 | 1.6 | 2.7 | 3.6 | 1.4 | 2.6 | 2.1 | 3.1 | 2.0 | 2.0 | 2.5 | -0.9 | -1.3 | 0.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.1 | -2.0 | -3.8 | -2.6 | -1.1 | -1.8 | 2.0 | -1.7 | -2.2 | 1.4 | 1.4 | 0.1 | 5.7 | 4.0 | 3.5 |
Mitochondria | -3.2 | 0.3 | 1.6 | -0.2 | -0.8 | 0.2 | -2.5 | 1.8 | 0.7 | -5.1 | -5.9 | -6.8 | 0 | -1.3 | -1.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 154.6261 | 159.2665 | 145.7397 | 136.5169 | 147.9427 | 163.5819 | 71.9145 | 86.7217 | 80.2092 | 75.3096 | 70.6166 | 87.9755 | 123.2457 | 138.0413 | 121.7444 | 131.1991 | 118.9839 | 141.3632 |
Actin | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0159 | 0.0086 | 0.0219 | 0.0017 | 0.0482 | 0.0002 | 0.0061 | 0.0002 | 0.0004 | 0.0001 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0016 | 0.0009 | 0.001 | 0.001 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0.0008 | 0.0004 | 0.0003 | 0.0001 |
Bud Periphery | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0007 | 0.0002 | 0.0006 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0094 | 0.0068 | 0.0037 | 0.0014 | 0.002 | 0 | 0.0001 | 0.0009 | 0.0002 | 0 | 0 | 0 |
Cell Periphery | 0.0053 | 0.0048 | 0.0221 | 0.0017 | 0.0019 | 0.008 | 0.0037 | 0.0043 | 0.0056 | 0.0087 | 0.0013 | 0.0172 | 0.0016 | 0.0045 | 0.0029 | 0.0036 | 0.0157 | 0.0006 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0.0133 | 0 | 0.0004 | 0.0002 | 0.0016 | 0.0007 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0 | 0 | 0 | 0 | 0 | 0 | 0.0253 | 0.0001 | 0.0036 | 0.0231 | 0.0248 | 0.003 | 0.0043 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.9933 | 0.9945 | 0.9705 | 0.9971 | 0.9975 | 0.9907 | 0.847 | 0.9778 | 0.9267 | 0.9289 | 0.8309 | 0.9597 | 0.9656 | 0.9932 | 0.995 | 0.9953 | 0.983 | 0.9992 |
Endoplasmic Reticulum | 0.0001 | 0 | 0.0019 | 0.0003 | 0.0001 | 0.0001 | 0.0015 | 0 | 0.0035 | 0.0014 | 0.0009 | 0.0004 | 0.0001 | 0 | 0 | 0.0002 | 0.0002 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0.0084 | 0.0001 | 0.0043 | 0.0001 | 0.0143 | 0.0137 | 0.0006 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0037 | 0.0004 | 0.0029 | 0.0004 | 0.0156 | 0.0015 | 0.0006 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Lipid Particles | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0077 | 0 | 0.0013 | 0.0047 | 0.0183 | 0.0002 | 0.0024 | 0 | 0 | 0 | 0.0001 | 0 |
Mitochondria | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.023 | 0.0004 | 0.0172 | 0.0136 | 0.0098 | 0.0002 | 0.0026 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
None | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0211 | 0 | 0.0029 | 0.0047 | 0.0016 | 0.0001 | 0.0034 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 |
Nuclear Periphery | 0.0003 | 0.0001 | 0.0029 | 0.0003 | 0.0002 | 0.0004 | 0.0018 | 0.0001 | 0.0006 | 0.0006 | 0.0035 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0027 | 0 | 0.0006 | 0.0017 | 0.0002 | 0 | 0.0056 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0012 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0069 | 0.0001 | 0.0021 | 0.0057 | 0.0224 | 0.0001 | 0.002 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0 | 0 | 0.0026 | 0 | 0.0001 | 0.0009 | 0.0025 | 0 | 0.0025 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0003 | 0.0001 | 0.0014 | 0 | 0 | 0.0001 | 0.0015 | 0 | 0.0004 | 0.0002 | 0.0003 | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 |
Vacuole Periphery | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 59.1864 | 179.5129 | 465.521 | 491.003 | 101.4035 | 128.8705 | 350.1142 | 550.6226 | 548.2529 | 224.1986 |
Translational Efficiency | 1.1689 | 1.382 | 1.5343 | 0.9295 | 1.5207 | 0.939 | 1.5608 | 1.5573 | 1.7319 | 1.6188 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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