Standard name
Human Ortholog
Description Type II integral membrane protein; required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p; KRE6 has a paralog, SKN1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0
Bud 0.05 0 0 0
Bud Neck 0 0 0 0
Bud Site 0 0
Cell Periphery 0 0 0 0
Cytoplasm 0.47 0.91 0.1 0
Endoplasmic Reticulum 0.05 0.06 0 0.08
Endosome 0 0 0.06 0.05
Golgi 0 0 0 0
Mitochondria 0.16 0 0 0.09
Nucleus 0.05 0 0 0
Nuclear Periphery 0 0 0 0
Nucleolus 0 0 0 0
Peroxisomes 0 0 0 0
SpindlePole 0 0 0 0
Vac/Vac Membrane 0.32 0.11 0.7 0.74
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0
Bud 1 0 0 0
Bud Neck 0 0 0 0
Bud Site 0 0
Cell Periphery 0 0 0 0
Cytoplasm 9 330 4 0
Endoplasmic Reticulum 1 23 0 0
Endosome 0 4 2 0
Golgi 0 2 0 0
Mitochondria 3 9 0 0
Nucleus 1 3 0 0
Nuclear Periphery 0 0 0 0
Nucleolus 0 0 0 0
Peroxisomes 0 0 0 0
SpindlePole 0 1 0 0
Vac/Vac Membrane 6 39 33 7
Unique Cell Count 19 364 48 10
Labelled Cell Count 21 411 48 10


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 3.8 4.3 5.1
Std Deviation (1e-4) 1.2 0.9 1.2 1.3
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.2916 0.0027 0.001 0.0001 0.0007 0.0099 0.465 0.001 0.0003 0.0001 0.0001 0.0005
Bud 0.0031 0.0025 0.0044 0.007 0.0014 0.0005 0.0012 0.0029 0.004 0.0036 0.0014 0.0011
Bud Neck 0.21 0.0012 0.0062 0.0022 0.0014 0.006 0.007 0.0046 0.0082 0.0043 0.0029 0.0081
Bud Periphery 0.004 0.0045 0.0068 0.0034 0.0028 0.0008 0.0018 0.0043 0.0064 0.0049 0.002 0.0021
Bud Site 0.1041 0.0057 0.0054 0.004 0.002 0.0008 0.003 0.0069 0.0105 0.0111 0.0025 0.0017
Cell Periphery 0.0026 0.0009 0.001 0.0008 0.0006 0.0008 0.0003 0.0005 0.0009 0.0007 0.0005 0.0008
Cytoplasm 0.0252 0.2747 0.2687 0.1176 0.1459 0.3155 0.0747 0.3381 0.2869 0.1887 0.1092 0.332
Cytoplasmic Foci 0.0173 0.0366 0.0313 0.0448 0.0826 0.0389 0.0215 0.0505 0.0517 0.04 0.0361 0.0374
Eisosomes 0.0019 0.0001 0 0 0.0001 0.0017 0.0047 0 0 0 0 0
Endoplasmic Reticulum 0.0148 0.0093 0.0089 0.0053 0.0046 0.0045 0.0119 0.0061 0.0075 0.0046 0.0039 0.0059
Endosome 0.2188 0.1128 0.1433 0.2702 0.2549 0.1339 0.1158 0.0918 0.1222 0.1644 0.1663 0.095
Golgi 0.014 0.0018 0.0053 0.0031 0.006 0.0019 0.0417 0.002 0.0025 0.0022 0.002 0.0009
Lipid Particles 0.0039 0.003 0.0028 0.0034 0.0033 0.0012 0.0391 0.0017 0.0016 0.0011 0.002 0.0014
Mitochondria 0.0141 0.0023 0.0082 0.002 0.0468 0.001 0.0169 0.0044 0.0081 0.0037 0.0036 0.0012
None 0.002 0.3587 0.2204 0.1022 0.0544 0.1183 0.021 0.3566 0.2429 0.1684 0.1273 0.1188
Nuclear Periphery 0.0171 0.005 0.0045 0.0048 0.0031 0.0028 0.0119 0.0051 0.0059 0.0053 0.0057 0.0053
Nucleolus 0.0138 0.001 0.001 0.0015 0.001 0.0007 0.0026 0.0017 0.0025 0.0026 0.0023 0.0015
Nucleus 0.0061 0.0049 0.008 0.0064 0.0056 0.009 0.0416 0.0098 0.0156 0.0153 0.0125 0.0174
Peroxisomes 0.003 0.0004 0.0019 0.0008 0.0044 0.0004 0.0374 0.0009 0.0025 0.001 0.0019 0.0006
Punctate Nuclear 0.0056 0.0013 0.0005 0.0007 0.0014 0.0022 0.0043 0.0014 0.0019 0.0011 0.0063 0.0028
Vacuole 0.0154 0.1581 0.2522 0.3873 0.3473 0.3295 0.07 0.1007 0.1989 0.3515 0.4729 0.3463
Vacuole Periphery 0.0117 0.0124 0.0182 0.0326 0.0298 0.0196 0.0067 0.0091 0.019 0.0253 0.0385 0.0192

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 190.5324 101.693 73.4302 84.1675 115.7453 128.9907 114.9251 96.9389 89.263 140.9201
Translational Efficiency 1.0148 1.1945 0.9012 0.7568 0.9747 1.352 0.9429 0.7598 0.7975 0.8253

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Type II integral membrane protein; required for beta-1,6 glucan biosynthesis; putative beta-glucan synthase; localizes to ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p; KRE6 has a paralog, SKN1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (16%), ER (4%), mixed (50%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Kre6

Kre6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kre6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available