Standard name | |
---|---|
Human Ortholog | |
Description | Glycogen phosphorylase required for the mobilization of glycogen; non-essential; regulated by cyclic AMP-mediated phosphorylation; phosphorylation by Cdc28p may coordinately regulate carbohydrate metabolism and the cell cycle; expression is regulated by stress-response elements and by the HOG MAP kinase pathway |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 4 | 6 | 20 | 16 | 29 | 5 | – | 16 | 32 | 3 | 12 | 10 | 0 | 1 | 3 | 0 | 2 | 4 | 3 | 10 | 8 |
Bud | 2 | 1 | 0 | 1 | 3 | 5 | 12 | 18 | 4 | – | 18 | 14 | 6 | 7 | 12 | 0 | 1 | 1 | 4 | 1 | 4 | 7 | 45 | 42 |
Bud Neck | 2 | 0 | 8 | 1 | 0 | 1 | 2 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 6 | 7 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 3 | 4 | 6 | 25 | 36 | 30 | 52 | 16 | – | 28 | 59 | 8 | 47 | 59 | 28 | 36 | 16 | 0 | 0 | 1 | 3 | 4 | 5 |
Cytoplasm | 276 | 282 | 307 | 191 | 291 | 229 | 287 | 272 | 126 | – | 126 | 164 | 453 | 346 | 391 | 519 | 639 | 489 | 264 | 278 | 251 | 250 | 334 | 253 |
Endoplasmic Reticulum | 0 | 1 | 0 | 1 | 5 | 15 | 17 | 18 | 6 | – | 7 | 16 | 2 | 4 | 20 | 24 | 36 | 25 | 0 | 1 | 2 | 5 | 13 | 8 |
Endosome | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 3 | 0 | – | 0 | 0 | 5 | 20 | 17 | 4 | 3 | 5 | 1 | 3 | 2 | 4 | 4 | 3 |
Golgi | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 2 | – | 2 | 1 | 1 | 6 | 9 | 3 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 3 |
Mitochondria | 3 | 6 | 0 | 5 | 5 | 29 | 49 | 57 | 63 | – | 113 | 105 | 5 | 5 | 13 | 6 | 6 | 9 | 3 | 4 | 18 | 41 | 42 | 25 |
Nucleus | 0 | 0 | 0 | 1 | 2 | 0 | 6 | 6 | 0 | – | 0 | 5 | 1 | 2 | 3 | 5 | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 3 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 5 | 15 | 11 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 0 | 0 | 5 | 1 | 1 | 2 | 2 | 1 | – | 0 | 1 | 5 | 7 | 5 | 0 | 0 | 1 | 2 | 3 | 4 | 3 | 11 | 12 |
Vac/Vac Membrane | 2 | 6 | 0 | 0 | 11 | 9 | 13 | 42 | 7 | – | 14 | 21 | 1 | 12 | 15 | 41 | 36 | 34 | 2 | 2 | 0 | 12 | 23 | 11 |
Unique Cell Count | 281 | 296 | 312 | 206 | 317 | 296 | 370 | 405 | 188 | 236 | 308 | 468 | 431 | 506 | 560 | 668 | 523 | 285 | 305 | 305 | 350 | 512 | 395 | |
Labelled Cell Count | 293 | 304 | 322 | 217 | 351 | 350 | 436 | 503 | 236 | 326 | 420 | 495 | 486 | 567 | 630 | 763 | 584 | 285 | 305 | 305 | 350 | 512 | 395 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 5.3 | 5.1 | 4.9 | 7.0 | 7.0 | 7.8 | 8.5 | 7.8 | – | 9.0 | 9.8 | 4.8 | 7.2 | 7.8 | 8.7 | 8.9 | 8.4 | 4.5 | 4.9 | 5.8 |
Std Deviation (1e-4) | 1.8 | 1.1 | 1.9 | 1.3 | 2.6 | 2.8 | 3.2 | 3.2 | 3.1 | – | 4.7 | 4.7 | 1.8 | 4.4 | 4.5 | 2.3 | 2.3 | 2.8 | 2.2 | 2.4 | 4.6 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.47 | 0.46 | 0.63 | 0.75 | 0.63 | – | 0.84 | 0.96 | -0.07 | 0.5 | 0.62 | 0.79 | 0.81 | 0.72 | -0.19 | -0.05 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 4.3 | 3.3 | 4.5 | 0 | – | 4.3 | 5.6 | 0 | 2.5 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 3.2 | 3.8 | 0 | – | 5.0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 3.9 | 5.4 | 4.1 | 5.7 | 4.0 | – | 5.2 | 7.4 | 0 | 5.1 | 5.4 | 2.8 | 3.0 | 1.6 |
Cytoplasm | -3.3 | -3.8 | -8.0 | -8.1 | -10.5 | -10.0 | – | -12.8 | -13.2 | -1.4 | -7.5 | -8.3 | -3.6 | -2.2 | -3.2 |
Endoplasmic Reticulum | 0 | 0 | 4.0 | 3.8 | 3.8 | 0 | – | 0 | 4.1 | 0 | 0 | 3.6 | 3.7 | 4.2 | 3.9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.5 | 2.9 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.7 | 6.7 | 6.9 | 10.9 | – | 13.7 | 11.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.3 | 0 | 3.3 | 5.9 | 0 | – | 4.4 | 4.7 | 0 | 3.0 | 3.1 | 4.9 | 4.2 | 4.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5416 | 5.5268 | 5.1547 | 5.842 | 3.813 | 4.2592 | 2.4812 | 3.9807 | 3.4173 | 2.6176 | 2.9899 | 3.371 | 3.8619 | 5.7424 | 4.8685 | 3.0578 | 5.9105 | 4.8276 |
Actin | 0.0158 | 0.0463 | 0.0512 | 0.2276 | 0.0004 | 0.0012 | 0.0251 | 0.0103 | 0.0171 | 0.0281 | 0.0063 | 0.0144 | 0.0285 | 0.0237 | 0.0211 | 0.0213 | 0.0387 | 0.0327 |
Bud | 0.0009 | 0.0017 | 0.0013 | 0.0006 | 0 | 0.0001 | 0.0064 | 0.0043 | 0.0096 | 0.0211 | 0.001 | 0.005 | 0.0162 | 0.0302 | 0.0293 | 0.025 | 0.0132 | 0.0173 |
Bud Neck | 0.0027 | 0.0008 | 0.0016 | 0.0057 | 0.0002 | 0.0009 | 0.0137 | 0.0006 | 0.0028 | 0.0017 | 0.002 | 0.0065 | 0.0016 | 0.0009 | 0.0016 | 0.0111 | 0.0062 | 0.0035 |
Bud Periphery | 0.0016 | 0.0031 | 0.0033 | 0.0012 | 0 | 0.0001 | 0.0043 | 0.0042 | 0.0101 | 0.0191 | 0.0012 | 0.0101 | 0.021 | 0.0232 | 0.0293 | 0.0306 | 0.0104 | 0.0333 |
Bud Site | 0.0015 | 0.0081 | 0.0216 | 0.0035 | 0 | 0 | 0.0224 | 0.0252 | 0.0159 | 0.0031 | 0.0021 | 0.0017 | 0.0216 | 0.0746 | 0.0288 | 0.0075 | 0.0065 | 0.0027 |
Cell Periphery | 0.0009 | 0.0062 | 0.0102 | 0.0006 | 0.0002 | 0.0001 | 0.005 | 0.0007 | 0.0007 | 0.0004 | 0.0004 | 0.0005 | 0.0042 | 0.0031 | 0.0016 | 0.0011 | 0.0009 | 0.0015 |
Cytoplasm | 0.5727 | 0.6893 | 0.6321 | 0.7007 | 0.7088 | 0.7947 | 0.5617 | 0.8445 | 0.7102 | 0.6172 | 0.6489 | 0.8028 | 0.6323 | 0.6822 | 0.6911 | 0.5298 | 0.7072 | 0.7024 |
Cytoplasmic Foci | 0.0278 | 0.0084 | 0.0524 | 0.0026 | 0.0017 | 0.0036 | 0.0453 | 0.0085 | 0.0243 | 0.0253 | 0.0351 | 0.036 | 0.0393 | 0.0283 | 0.0372 | 0.0668 | 0.0499 | 0.0512 |
Eisosomes | 0.0004 | 0.0011 | 0.002 | 0.0025 | 0.0001 | 0 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0114 | 0.0043 | 0.0031 | 0.0019 | 0.0056 | 0.0028 | 0.0135 | 0.0026 | 0.0034 | 0.0035 | 0.0046 | 0.0058 | 0.0094 | 0.0031 | 0.0061 | 0.0084 | 0.0042 | 0.005 |
Endosome | 0.0273 | 0.0024 | 0.0227 | 0.0027 | 0.0004 | 0.0016 | 0.0626 | 0.0018 | 0.0195 | 0.0194 | 0.041 | 0.0102 | 0.0109 | 0.0036 | 0.0052 | 0.0564 | 0.0081 | 0.0138 |
Golgi | 0.0027 | 0.0036 | 0.0034 | 0.0026 | 0.0001 | 0.0002 | 0.0143 | 0.0007 | 0.0044 | 0.0127 | 0.0149 | 0.0026 | 0.0034 | 0.0013 | 0.0018 | 0.0067 | 0.0021 | 0.0028 |
Lipid Particles | 0.0161 | 0.0007 | 0.0027 | 0.0013 | 0.0002 | 0 | 0.0095 | 0.0005 | 0.0022 | 0.0019 | 0.0101 | 0.0007 | 0.0028 | 0.0004 | 0.0008 | 0.0067 | 0.0008 | 0.0005 |
Mitochondria | 0.0016 | 0.0167 | 0.0271 | 0.0004 | 0 | 0.0001 | 0.0075 | 0.0002 | 0.0062 | 0.0129 | 0.004 | 0.0046 | 0.0022 | 0.0013 | 0.001 | 0.0015 | 0.0011 | 0.0013 |
None | 0.2627 | 0.2013 | 0.1529 | 0.0421 | 0.281 | 0.1932 | 0.1403 | 0.0811 | 0.1494 | 0.2041 | 0.0885 | 0.0816 | 0.1835 | 0.1081 | 0.1124 | 0.1765 | 0.122 | 0.1158 |
Nuclear Periphery | 0.0087 | 0.0007 | 0.0005 | 0.0006 | 0.0003 | 0.0002 | 0.0142 | 0.0006 | 0.0024 | 0.0051 | 0.0389 | 0.0041 | 0.002 | 0.0014 | 0.0022 | 0.003 | 0.0022 | 0.0016 |
Nucleolus | 0.0071 | 0.0002 | 0.0003 | 0.0001 | 0 | 0 | 0.0053 | 0.0001 | 0.0009 | 0.0005 | 0.0443 | 0.0003 | 0.0007 | 0.0009 | 0.0022 | 0.0044 | 0.0005 | 0.0001 |
Nucleus | 0.0083 | 0.0008 | 0.0009 | 0.0005 | 0.0005 | 0.0005 | 0.0113 | 0.0022 | 0.0104 | 0.009 | 0.0143 | 0.0048 | 0.0037 | 0.0059 | 0.007 | 0.0062 | 0.0047 | 0.0029 |
Peroxisomes | 0.0118 | 0.0014 | 0.0057 | 0.0014 | 0 | 0 | 0.0077 | 0.0002 | 0.0014 | 0.0039 | 0.0278 | 0.0016 | 0.0019 | 0.0023 | 0.0018 | 0.0073 | 0.0104 | 0.0033 |
Punctate Nuclear | 0.0149 | 0.0003 | 0.0015 | 0.0003 | 0.0002 | 0.0001 | 0.0125 | 0.0093 | 0.0027 | 0.0052 | 0.0016 | 0.0038 | 0.0085 | 0.0025 | 0.0165 | 0.0173 | 0.0036 | 0.003 |
Vacuole | 0.0025 | 0.0017 | 0.0031 | 0.001 | 0.0003 | 0.0004 | 0.0142 | 0.0019 | 0.0052 | 0.0044 | 0.0094 | 0.0024 | 0.0052 | 0.0027 | 0.0026 | 0.0109 | 0.0065 | 0.0044 |
Vacuole Periphery | 0.0005 | 0.0007 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0029 | 0.0001 | 0.001 | 0.0013 | 0.0035 | 0.0007 | 0.0006 | 0.0003 | 0.0004 | 0.0013 | 0.0006 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.7755 | 5.0976 | 7.8354 | 14.6007 | 8.1855 | 8.9559 | 8.8356 | 8.5725 | 18.6124 | 16.0028 |
Translational Efficiency | 1.0081 | 1.0534 | 0.7089 | 0.8876 | 1.2845 | 1.4308 | 0.5841 | 0.8045 | 0.8617 | 0.7837 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1774 | 1429 | 233 | 1376 | 117 | 1590 | 146 | 36 | 1891 | 3019 | 379 | 1412 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 623.36 | 716.93 | 3351.78 | 838.67 | 638.62 | 666.42 | 1131.30 | 1034.10 | 624.30 | 690.33 | 2496.40 | 843.65 |
Standard Deviation | 71.64 | 122.67 | 1366.42 | 175.55 | 66.79 | 103.43 | 457.41 | 390.83 | 71.44 | 115.73 | 1547.94 | 186.75 |
Intensity Change Log 2 | — | 0.201767 | 2.426790 | 0.428038 | — | 0.061474 | 0.824952 | 0.695346 | — | 0.132477 | 1.828801 | 0.569485 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000113 | 0.000862 | 0.193604* | 0.008819 | 0.000205 | 0.000490 | 0.045066 | 0.018855 | 0.000119 | 0.000666 | 0.136384* | 0.009074 |
Bud Neck | 0.015254 | 0.028538 | 0.050176 | 0.013731 | 0.019285 | 0.033617 | 0.007413 | 0.011258 | 0.015503 | 0.031213 | 0.033703 | 0.013668 |
Bud Site | 0.006161 | 0.015229 | 0.330880* | 0.062821 | 0.008525 | 0.019532 | 0.077062 | 0.269546* | 0.006307 | 0.017495 | 0.233103* | 0.068092 |
Cell Periphery | 0.000149 | 0.000114 | 0.089539 | 0.003011 | 0.002050 | 0.000158 | 0.004785 | 0.008862 | 0.000266 | 0.000137 | 0.056890 | 0.003161 |
Cytoplasm | 0.528906* | 0.477050* | 0.031189 | 0.449816* | 0.358613* | 0.447261* | 0.317562* | 0.341664* | 0.518370* | 0.461361* | 0.141507* | 0.447059* |
Cytoplasmic Foci | 0.155371 | 0.210292* | 0.074126 | 0.025333 | 0.304769* | 0.210188* | 0.048811 | 0.016608 | 0.164614 | 0.210237* | 0.064374 | 0.025111 |
Eisosomes | 0.000125 | 0.000089 | 0.011915 | 0.000081 | 0.000659 | 0.000111 | 0.000221 | 0.000116 | 0.000158 | 0.000100 | 0.007410 | 0.000082 |
Endoplasmic Reticulum | 0.000622 | 0.000662 | 0.009523 | 0.002164 | 0.000428 | 0.001026 | 0.003151 | 0.000727 | 0.000610 | 0.000854 | 0.007068 | 0.002127 |
Endosome | 0.006700 | 0.021383 | 0.012111 | 0.008933 | 0.024164 | 0.021366 | 0.008856 | 0.004419 | 0.007781 | 0.021374 | 0.010857 | 0.008818 |
Golgi | 0.000963 | 0.004811 | 0.016000 | 0.004370 | 0.002459 | 0.004185 | 0.001224 | 0.000579 | 0.001056 | 0.004481 | 0.010308 | 0.004273 |
Lipid Particles | 0.006020 | 0.005179 | 0.087604 | 0.002234 | 0.013123 | 0.005379 | 0.012923 | 0.001732 | 0.006460 | 0.005284 | 0.058835 | 0.002221 |
Mitochondria | 0.002609 | 0.010885 | 0.001380 | 0.003353 | 0.005022 | 0.013576 | 0.001661 | 0.000833 | 0.002758 | 0.012302 | 0.001488 | 0.003288 |
Mitotic Spindle | 0.002369 | 0.008472 | 0.003733 | 0.082717 | 0.000214 | 0.006066 | 0.007168 | 0.088522 | 0.002236 | 0.007205 | 0.005056 | 0.082865 |
None | 0.004653 | 0.003081 | 0.000212 | 0.004554 | 0.009661 | 0.005120 | 0.002984 | 0.002502 | 0.004963 | 0.004155 | 0.001280 | 0.004502 |
Nuclear Periphery | 0.000452 | 0.000464 | 0.000721 | 0.001503 | 0.000483 | 0.000773 | 0.001027 | 0.000344 | 0.000454 | 0.000627 | 0.000839 | 0.001474 |
Nuclear Periphery Foci | 0.000340 | 0.000466 | 0.002072 | 0.001431 | 0.000409 | 0.000646 | 0.005232 | 0.000700 | 0.000344 | 0.000561 | 0.003289 | 0.001412 |
Nucleolus | 0.001365 | 0.002260 | 0.000056 | 0.000851 | 0.001297 | 0.001829 | 0.000382 | 0.000345 | 0.001360 | 0.002033 | 0.000182 | 0.000838 |
Nucleus | 0.225058* | 0.122492 | 0.004262 | 0.193419* | 0.179897* | 0.138954* | 0.192210* | 0.055672 | 0.222264* | 0.131162* | 0.076664 | 0.189907* |
Peroxisomes | 0.003427 | 0.011111 | 0.002533 | 0.004967 | 0.020769 | 0.012061 | 0.005206 | 0.013723 | 0.004500 | 0.011611 | 0.003563 | 0.005190 |
Vacuole | 0.038653 | 0.073886 | 0.075876 | 0.120600 | 0.046837 | 0.074594 | 0.255585* | 0.162419 | 0.039159 | 0.074259 | 0.145104 | 0.121666 |
Vacuole Periphery | 0.000691 | 0.002674 | 0.002486 | 0.005292 | 0.001130 | 0.003067 | 0.001470 | 0.000575 | 0.000718 | 0.002881 | 0.002095 | 0.005171 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.47 | -10.78 | -4.19 | -2.97 | 10.52 | -1.67 | -1.87 | -1.55 | -1.50 | 0.70 | -2.15 | -10.14 | -4.37 | -3.64 | 9.76 |
Bud Neck | -5.79 | -9.61 | 1.19 | 6.61 | 10.13 | -3.05 | 3.15 | 1.09 | 3.88 | -1.21 | -8.56 | -6.47 | 1.39 | 9.69 | 7.26 |
Bud Site | -5.42 | -17.66 | -10.18 | -6.76 | 15.83* | -1.74 | -2.41 | -3.23 | -3.03 | -2.60 | -7.26 | -15.06 | -10.49 | -6.79 | 12.42 |
Cell Periphery | 2.23 | -8.00 | -1.43 | -1.49 | 7.89 | 1.12 | 0.17 | -0.39 | -1.01 | -0.51 | 1.25 | -7.69 | -1.35 | -1.57 | 7.51 |
Cytoplasm | 5.35 | 42.33 | 16.15 | 9.76 | -26.33 | -3.58 | 2.29 | 1.24 | 3.19 | -0.15 | 7.13 | 28.40 | 15.55 | 10.06 | -16.11 |
Cytoplasmic Foci | -7.69 | 5.91 | 30.56 | 33.65 | 5.53 | 4.54 | 12.49 | 13.97 | 26.73 | 2.64 | -7.74 | 11.28 | 32.68 | 46.77 | 6.05 |
Eisosomes | 5.20 | -3.73 | 5.99 | 1.47 | 3.75 | 1.81 | 1.61 | 1.91 | 1.15 | 2.65 | 2.91 | -3.68 | 3.86 | 3.58 | 3.73 |
Endoplasmic Reticulum | -0.59 | -4.72 | -13.55 | -14.74 | 3.39 | -1.97 | -7.99 | -2.71 | -1.21 | 4.15 | -1.48 | -6.03 | -13.89 | -9.45 | 3.92 |
Endosome | -10.03 | -1.84 | 2.51 | 11.54 | 2.75 | 0.42 | 2.80 | 2.59 | 5.08 | -0.06 | -11.24 | -0.99 | 3.46 | 14.53 | 3.02 |
Golgi | -5.84 | -3.98 | -1.29 | 4.14 | 3.79 | -2.25 | 3.01 | 3.50 | 7.36 | 0.95 | -8.10 | -3.82 | -1.05 | 4.99 | 3.55 |
Lipid Particles | 1.18 | -7.54 | 6.22 | 6.15 | 7.92 | 4.10 | 2.38 | 5.80 | 4.64 | 2.18 | 1.81 | -7.13 | 7.20 | 8.12 | 7.78 |
Mitochondria | -6.66 | 3.89 | 1.67 | 7.24 | -1.80 | -3.81 | 2.28 | 2.45 | 9.42 | 0.34 | -9.83 | 4.54 | 2.09 | 10.78 | -2.14 |
Mitotic Spindle | -3.15 | -1.81 | -11.18 | -9.44 | -10.42 | -4.42 | -2.56 | -2.40 | -2.22 | -2.00 | -3.76 | -2.66 | -11.46 | -10.27 | -9.41 |
None | 2.20 | 8.12 | -0.49 | -3.10 | -13.00 | 1.04 | 1.66 | 1.64 | 1.81 | 0.05 | 1.11 | 6.22 | 0.08 | -1.26 | -8.86 |
Nuclear Periphery | -0.39 | -3.68 | -16.25 | -16.70 | -12.82 | -1.49 | -6.92 | -1.81 | -1.56 | -0.91 | -1.66 | -7.11 | -16.31 | -12.48 | -10.56 |
Nuclear Periphery Foci | -0.94 | -5.72 | -3.82 | -3.47 | 0.75 | -0.76 | -5.43 | -1.67 | -1.55 | 1.65 | -1.29 | -7.24 | -3.98 | -3.46 | 3.14 |
Nucleolus | -2.28 | 4.77 | -1.13 | 0.75 | -4.69 | -1.66 | 1.16 | 0.68 | 1.69 | -0.32 | -2.12 | 3.43 | -1.13 | 0.33 | -3.76 |
Nucleus | 14.63 | 39.36 | -3.06 | -15.78 | -35.27 | 1.93 | -0.17 | 2.94 | 2.14 | 3.20 | 14.77 | 14.60 | -2.93 | -15.49 | -15.73 |
Peroxisomes | -7.33 | 1.37 | 1.81 | 8.43 | 0.02 | 1.47 | 2.77 | 1.68 | 0.87 | -0.52 | -8.01 | 1.65 | 2.84 | 11.01 | 0.61 |
Vacuole | -10.79 | -4.87 | -25.75 | -19.44 | -11.67 | -2.96 | -12.48 | -4.94 | -4.46 | 1.03 | -13.26 | -11.80 | -26.14 | -20.75 | -1.72 |
Vacuole Periphery | -6.20 | -4.20 | -1.88 | 1.09 | 0.80 | -3.43 | 1.02 | 1.72 | 4.78 | 0.20 | -7.07 | -3.32 | -1.83 | 1.48 | -0.11 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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