Standard name | |
---|---|
Human Ortholog | |
Description | Translation initiation factor eIF-4B; contains an RNA recognition motif and binds to single-stranded RNA; has RNA annealing activity; interacts with Rps20p at the head of the 40S ribosomal subunit and alters the structure of the mRNA entry channel |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 6 | 24 | 20 | 29 | 41 | 70 | 93 | 91 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 0 | 0 | 2 | 0 | 2 | 4 | 4 | 2 | 6 | 1 | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 5 | 1 | 8 | 9 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 9 | 11 | 20 | 6 | 18 | 44 | 43 | 47 | 72 | 67 | 72 | 4 | 12 | 20 | 14 | 10 | 6 | 0 | 1 | 4 | 0 | 0 | 2 |
Cytoplasm | 152 | 545 | 500 | 382 | 300 | 485 | 436 | 375 | 358 | 375 | 321 | 299 | 388 | 318 | 448 | 515 | 446 | 291 | 153 | 530 | 513 | 378 | 565 | 468 |
Endoplasmic Reticulum | 0 | 6 | 36 | 18 | 36 | 82 | 174 | 192 | 199 | 301 | 210 | 222 | 7 | 7 | 8 | 30 | 28 | 13 | 1 | 3 | 38 | 2 | 4 | 11 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Golgi | 0 | 0 | 1 | 6 | 2 | 6 | 4 | 38 | 36 | 48 | 56 | 92 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 1 | 0 | 0 | 2 | 29 | 31 | 25 | 34 | 81 | 77 | 91 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 2 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 4 | 4 | 1 | 1 | 12 | 5 | 6 | 3 | 10 | 1 | 0 | 1 | 3 | 3 | 4 | 0 | 2 | 4 | 6 | 2 | 6 |
Unique Cell Count | 153 | 553 | 534 | 413 | 349 | 603 | 668 | 653 | 644 | 804 | 670 | 697 | 393 | 327 | 456 | 527 | 452 | 295 | 159 | 553 | 570 | 400 | 587 | 515 |
Labelled Cell Count | 156 | 563 | 548 | 433 | 357 | 648 | 714 | 720 | 724 | 959 | 828 | 884 | 402 | 341 | 478 | 565 | 488 | 315 | 159 | 553 | 570 | 400 | 587 | 515 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 47.1 | 71.0 | 56.1 | 47.3 | 38.2 | 35.6 | 33.5 | 33.1 | 32.9 | 32.2 | 31.4 | 30.3 | 67.0 | 59.8 | 56.7 | 80.4 | 73.8 | 78.0 | 63.9 | 67.7 | 69.0 |
Std Deviation (1e-4) | 11.2 | 13.6 | 9.8 | 9.1 | 8.0 | 6.6 | 6.4 | 7.2 | 5.9 | 6.0 | 5.5 | 6.0 | 15.0 | 14.0 | 12.3 | 29.2 | 23.0 | 25.8 | 19.0 | 16.1 | 19.8 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.55 | -0.66 | -0.74 | -0.76 | -0.77 | -0.8 | -0.84 | -0.89 | 0.26 | 0.09 | 0.02 | 0.52 | 0.4 | 0.48 | 0.19 | 0.27 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 4.7 | 4.0 | 4.9 | 5.9 | 7.0 | 9.0 | 8.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.4 | -0.4 | 1.0 | 3.7 | 3.7 | 4.1 | 5.1 | 5.6 | 5.7 | -1.2 | 1.4 | 2.1 | 0.6 | 0.2 | 0 |
Cytoplasm | -0.7 | -3.8 | -6.5 | -11.8 | -14.1 | -14.6 | -17.7 | -16.9 | -18.5 | 3.8 | 2.4 | 3.6 | 3.3 | 4.0 | 3.3 |
Endoplasmic Reticulum | -1.6 | 1.9 | 3.8 | 8.8 | 9.9 | 10.3 | 12.7 | 10.5 | 10.7 | -3.5 | -3.0 | -3.8 | -0.7 | -0.3 | -1.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 5.4 | 5.3 | 5.5 | 6.6 | 8.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.1 | 5.0 | 4.6 | 5.4 | 7.6 | 8.1 | 8.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 42.6076 | 65.1223 | 59.8975 | 51.3738 | 51.5682 | 58.0934 | 65.9452 | 78.3424 | 75.7042 | 72.4578 | 61.1164 | 73.792 | 72.7364 | 80.817 | 78.1951 | 75.9165 | 69.9715 | 75.2632 |
Actin | 0.0068 | 0.0002 | 0.0167 | 0.0244 | 0.008 | 0.0041 | 0.0053 | 0.0001 | 0.0002 | 0 | 0.0012 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 |
Bud | 0.001 | 0.0003 | 0.0009 | 0.0011 | 0.001 | 0.0006 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0017 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0002 |
Bud Neck | 0.0058 | 0.0003 | 0.0006 | 0.0008 | 0.0006 | 0.0016 | 0.002 | 0.0004 | 0.0007 | 0.0006 | 0.0005 | 0.0012 | 0.0005 | 0.0005 | 0.0006 | 0.001 | 0.0007 | 0.0017 |
Bud Periphery | 0.0009 | 0.0001 | 0.0007 | 0.0008 | 0.0007 | 0.0004 | 0.0014 | 0.0002 | 0.0004 | 0.0002 | 0.0008 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0003 |
Bud Site | 0.0021 | 0.0003 | 0.0019 | 0.0006 | 0.0023 | 0.0002 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 |
Cell Periphery | 0.0026 | 0.0021 | 0.0008 | 0.0014 | 0.0008 | 0.0009 | 0.0169 | 0.012 | 0.0104 | 0.0198 | 0.0004 | 0.0044 | 0.0179 | 0.0105 | 0.0167 | 0.0038 | 0.0011 | 0.0065 |
Cytoplasm | 0.7011 | 0.9587 | 0.8882 | 0.7553 | 0.8353 | 0.8716 | 0.7789 | 0.9329 | 0.8786 | 0.7833 | 0.8417 | 0.8391 | 0.7822 | 0.918 | 0.8845 | 0.7964 | 0.888 | 0.8878 |
Cytoplasmic Foci | 0.0108 | 0.0008 | 0.0068 | 0.0268 | 0.011 | 0.0061 | 0.0011 | 0.0007 | 0.0037 | 0.0014 | 0.007 | 0.0025 | 0.0008 | 0.0008 | 0.001 | 0.0015 | 0.0022 | 0.0022 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0592 | 0.0033 | 0.0041 | 0.0047 | 0.0042 | 0.0414 | 0.0769 | 0.0184 | 0.02 | 0.0052 | 0.0055 | 0.1138 | 0.094 | 0.0207 | 0.0275 | 0.0573 | 0.0266 | 0.063 |
Endosome | 0.018 | 0.001 | 0.006 | 0.0105 | 0.0174 | 0.0165 | 0.0047 | 0.001 | 0.0027 | 0.0059 | 0.0072 | 0.0063 | 0.0031 | 0.0016 | 0.0023 | 0.0078 | 0.0088 | 0.0077 |
Golgi | 0.0026 | 0.0001 | 0.0013 | 0.0015 | 0.0019 | 0.003 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0031 | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.001 |
Lipid Particles | 0.0049 | 0.0002 | 0.0013 | 0.0108 | 0.0027 | 0.002 | 0.0012 | 0.0015 | 0.0043 | 0.0029 | 0.0014 | 0.001 | 0.001 | 0.0019 | 0.0017 | 0.0012 | 0.0006 | 0.0007 |
Mitochondria | 0.0047 | 0.0002 | 0.0013 | 0.015 | 0.0129 | 0.0039 | 0.0012 | 0.0001 | 0.0002 | 0.0001 | 0.0749 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0005 |
None | 0.0116 | 0.0003 | 0.0035 | 0.0052 | 0.0066 | 0.0043 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0047 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 |
Nuclear Periphery | 0.021 | 0.0014 | 0.0043 | 0.0059 | 0.0101 | 0.0064 | 0.0084 | 0.0014 | 0.0026 | 0.0042 | 0.0026 | 0.006 | 0.0075 | 0.0021 | 0.0031 | 0.0049 | 0.0054 | 0.003 |
Nucleolus | 0.0067 | 0.0016 | 0.0003 | 0.0039 | 0.0176 | 0.0027 | 0.0014 | 0.0001 | 0.0004 | 0.0002 | 0.004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0213 | 0.0025 | 0.0023 | 0.0055 | 0.0091 | 0.0029 | 0.0046 | 0.0011 | 0.0019 | 0.0021 | 0.0099 | 0.0018 | 0.002 | 0.0013 | 0.0013 | 0.0017 | 0.0018 | 0.0014 |
Peroxisomes | 0.0084 | 0 | 0.0009 | 0.0037 | 0.0012 | 0.0002 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0053 | 0.0001 | 0.0009 | 0.0088 | 0.0015 | 0.0004 | 0.0001 | 0 | 0.0003 | 0 | 0.001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Vacuole | 0.0864 | 0.0245 | 0.0475 | 0.0893 | 0.0438 | 0.0236 | 0.0843 | 0.0285 | 0.0686 | 0.157 | 0.0246 | 0.0182 | 0.0854 | 0.0396 | 0.0573 | 0.1089 | 0.0559 | 0.0212 |
Vacuole Periphery | 0.0183 | 0.0021 | 0.0096 | 0.0238 | 0.0109 | 0.0072 | 0.0087 | 0.0009 | 0.0033 | 0.0166 | 0.0055 | 0.0028 | 0.0034 | 0.0011 | 0.0025 | 0.0142 | 0.0075 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 352.6518 | 407.7924 | 400.3024 | 281.3631 | 327.3712 | 370.1214 | 294.2144 | 296.7712 | 303.124 | 244.9416 |
Translational Efficiency | 2.3443 | 2.3487 | 2.0347 | 3.1537 | 2.4031 | 1.9068 | 2.0296 | 2.1467 | 1.7251 | 2.1279 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1520 | 1014 | 2141 | 1361 | 1808 | 1306 | 797 | 2695 | 3328 | 2320 | 2938 | 4056 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3097.44 | 4334.94 | 4323.62 | 5353.89 | 3510.37 | 3936.32 | 4444.30 | 5829.62 | 3321.77 | 4110.54 | 4356.36 | 5669.99 |
Standard Deviation | 805.53 | 959.78 | 903.26 | 1062.71 | 703.35 | 842.26 | 855.44 | 1248.83 | 779.38 | 917.09 | 892.16 | 1210.65 |
Intensity Change Log 2 | — | 0.484936 | 0.481163 | 0.789511 | — | 0.165224 | 0.340333 | 0.731779 | — | 0.323935 | 0.408062 | 0.759129 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000066 | 0.000140 | 0.000090 | 0.000212 | 0.000047 | 0.000626 | 0.000191 | 0.000168 | 0.000056 | 0.000414 | 0.000118 | 0.000183 |
Bud Neck | 0.000253 | 0.000929 | 0.000772 | 0.001810 | 0.000201 | 0.000774 | 0.005379 | 0.000743 | 0.000225 | 0.000842 | 0.002022 | 0.001101 |
Bud Site | 0.002662 | 0.005468 | 0.004946 | 0.010440 | 0.002210 | 0.004208 | 0.009895 | 0.010906 | 0.002416 | 0.004759 | 0.006288 | 0.010750 |
Cell Periphery | 0.007380 | 0.014798 | 0.008389 | 0.026434 | 0.004166 | 0.004449 | 0.007449 | 0.013214 | 0.005634 | 0.008972 | 0.008134 | 0.017650 |
Cytoplasm | 0.756678* | 0.663332* | 0.788202* | 0.532903* | 0.797899* | 0.796066* | 0.531537* | 0.747356* | 0.779072* | 0.738052* | 0.718576* | 0.675396* |
Cytoplasmic Foci | 0.015062 | 0.018369 | 0.007633 | 0.006288 | 0.015729 | 0.011267 | 0.013186 | 0.006210 | 0.015424 | 0.014371 | 0.009139 | 0.006236 |
Eisosomes | 0.000001 | 0.000003 | 0.000002 | 0.000004 | 0.000001 | 0.000003 | 0.000004 | 0.000003 | 0.000001 | 0.000003 | 0.000002 | 0.000003 |
Endoplasmic Reticulum | 0.001573 | 0.000765 | 0.000616 | 0.000502 | 0.001244 | 0.000541 | 0.000341 | 0.000607 | 0.001394 | 0.000639 | 0.000541 | 0.000572 |
Endosome | 0.000998 | 0.001383 | 0.000793 | 0.001393 | 0.000862 | 0.001161 | 0.001863 | 0.001039 | 0.000924 | 0.001258 | 0.001083 | 0.001158 |
Golgi | 0.073031 | 0.102592 | 0.021762 | 0.022727 | 0.054876 | 0.050732 | 0.032414 | 0.008928 | 0.063168 | 0.073398 | 0.024652 | 0.013558 |
Lipid Particles | 0.007423 | 0.017215 | 0.011482 | 0.022866 | 0.004821 | 0.007184 | 0.016346 | 0.011441 | 0.006009 | 0.011568 | 0.012802 | 0.015274 |
Mitochondria | 0.000263 | 0.000353 | 0.000170 | 0.000703 | 0.000352 | 0.000400 | 0.000157 | 0.000933 | 0.000312 | 0.000379 | 0.000166 | 0.000856 |
Mitotic Spindle | 0.000898 | 0.002720 | 0.001056 | 0.001007 | 0.001340 | 0.002857 | 0.002958 | 0.000314 | 0.001138 | 0.002797 | 0.001572 | 0.000546 |
None | 0.005104 | 0.002637 | 0.000852 | 0.000811 | 0.002333 | 0.002791 | 0.001037 | 0.001432 | 0.003599 | 0.002724 | 0.000902 | 0.001224 |
Nuclear Periphery | 0.000282 | 0.000740 | 0.000318 | 0.000391 | 0.000265 | 0.000340 | 0.000951 | 0.000084 | 0.000273 | 0.000515 | 0.000490 | 0.000187 |
Nuclear Periphery Foci | 0.011221 | 0.020314 | 0.007514 | 0.006414 | 0.009658 | 0.010741 | 0.014047 | 0.001870 | 0.010372 | 0.014925 | 0.009286 | 0.003395 |
Nucleolus | 0.000090 | 0.000137 | 0.000068 | 0.000361 | 0.000034 | 0.000134 | 0.000195 | 0.000213 | 0.000060 | 0.000135 | 0.000102 | 0.000263 |
Nucleus | 0.006326 | 0.008124 | 0.006471 | 0.008898 | 0.002532 | 0.010343 | 0.028222 | 0.002919 | 0.004265 | 0.009373 | 0.012372 | 0.004925 |
Peroxisomes | 0.000022 | 0.000384 | 0.000051 | 0.000120 | 0.000016 | 0.000027 | 0.000039 | 0.000331 | 0.000019 | 0.000183 | 0.000048 | 0.000260 |
Vacuole | 0.040937 | 0.073779 | 0.100132 | 0.299885* | 0.032446 | 0.054199 | 0.287118* | 0.154261 | 0.036324 | 0.062757 | 0.150857 | 0.203125* |
Vacuole Periphery | 0.069730 | 0.065818 | 0.038680 | 0.055831 | 0.068967 | 0.041156 | 0.046670 | 0.037028 | 0.069315 | 0.051935 | 0.040847 | 0.043337 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.40 | -2.44 | -5.98 | -2.99 | -4.93 | -1.19 | -7.61 | -3.95 | 0.92 | 2.11 | -1.32 | -7.42 | -5.92 | 0.78 | -2.05 |
Bud Neck | -6.76 | -6.44 | -8.01 | -5.15 | -5.14 | -6.44 | -10.49 | -7.14 | 0.18 | 9.67 | -9.20 | -11.77 | -10.43 | -3.62 | 6.56 |
Bud Site | -4.29 | -4.39 | -7.61 | -4.62 | -4.96 | -4.22 | -6.94 | -11.69 | -7.78 | 0.85 | -5.98 | -8.27 | -13.79 | -8.79 | -4.78 |
Cell Periphery | -6.87 | -1.38 | -9.72 | -4.91 | -9.08 | -0.64 | -4.04 | -10.52 | -9.44 | -6.01 | -5.96 | -4.43 | -13.49 | -8.47 | -9.70 |
Cytoplasm | 7.19 | -2.13 | 18.37 | 8.99 | 21.57 | 0.57 | 20.85 | 6.46 | 5.19 | -17.32 | 5.34 | 9.56 | 14.93 | 7.56 | 3.97 |
Cytoplasmic Foci | -2.35 | 5.92 | 7.02 | 10.24 | 2.18 | 3.46 | -0.36 | 9.35 | 6.67 | 8.40 | 0.74 | 6.12 | 11.64 | 12.09 | 7.20 |
Eisosomes | -3.65 | -2.78 | -5.90 | -2.68 | -4.82 | -1.31 | -10.06 | -5.76 | 0.12 | 3.52 | -1.92 | -9.23 | -7.96 | -0.46 | -2.52 |
Endoplasmic Reticulum | 2.56 | 3.03 | 3.54 | 3.85 | 2.24 | 6.00 | 8.34 | 5.46 | -0.74 | -3.64 | 4.80 | 5.41 | 5.32 | 1.31 | -0.20 |
Endosome | -3.35 | 1.95 | -1.11 | 0.18 | -1.69 | -2.31 | -8.92 | -1.73 | 0.86 | 6.53 | -3.71 | -4.11 | -1.76 | 0.91 | 0.33 |
Golgi | -5.39 | 14.43 | 14.52 | 18.46 | 0.64 | 1.20 | 8.82 | 18.14 | 15.87 | 11.22 | -3.17 | 17.74 | 23.59 | 24.34 | 11.09 |
Lipid Particles | -7.00 | -4.36 | -9.16 | -2.23 | -6.12 | -2.75 | -8.33 | -7.12 | -3.56 | 3.36 | -7.00 | -9.35 | -10.72 | -3.06 | -1.59 |
Mitochondria | -0.58 | 2.08 | -1.27 | -0.89 | -1.58 | -0.29 | 1.52 | -2.53 | -2.07 | -3.58 | -0.59 | 2.27 | -2.90 | -2.24 | -3.84 |
Mitotic Spindle | -5.04 | -1.41 | -0.39 | 3.48 | 0.45 | -1.92 | -3.51 | 4.11 | 3.25 | 5.44 | -3.52 | -2.74 | 3.15 | 4.68 | 4.96 |
None | 2.02 | 3.93 | 3.94 | 3.12 | 0.23 | -0.59 | 2.01 | 1.27 | 2.21 | -1.06 | 1.22 | 4.48 | 3.71 | 3.37 | -1.38 |
Nuclear Periphery | -7.87 | -1.66 | -3.00 | 6.04 | -1.55 | -2.14 | -7.38 | 7.32 | 7.62 | 8.95 | -7.32 | -7.10 | 3.76 | 10.50 | 9.59 |
Nuclear Periphery Foci | -6.22 | 3.92 | 5.84 | 10.30 | 2.66 | -1.23 | -4.18 | 12.34 | 11.28 | 12.81 | -5.45 | 1.07 | 14.00 | 15.61 | 14.25 |
Nucleolus | -0.76 | 1.19 | -2.37 | -1.79 | -2.59 | -4.11 | -10.61 | -12.78 | -2.84 | 1.77 | -2.48 | -5.65 | -4.98 | -2.63 | -3.57 |
Nucleus | -1.15 | -0.77 | -3.28 | -2.54 | -3.23 | -8.44 | -11.45 | -2.64 | 7.23 | 11.09 | -6.27 | -10.36 | -2.92 | 3.83 | 8.55 |
Peroxisomes | -1.01 | -4.72 | -7.59 | 0.78 | -4.89 | -1.75 | -5.81 | -10.61 | -9.92 | -9.39 | -1.06 | -7.02 | -11.85 | -0.35 | -10.11 |
Vacuole | -7.33 | -14.37 | -32.63 | -26.51 | -22.78 | -5.98 | -24.83 | -27.08 | -18.49 | 12.54 | -9.26 | -26.67 | -40.27 | -29.99 | -7.98 |
Vacuole Periphery | 0.88 | 8.63 | 4.63 | 3.35 | -4.19 | 7.12 | 7.08 | 9.57 | 1.58 | 0.94 | 5.84 | 11.36 | 10.99 | 4.22 | -1.39 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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