Standard name
Human Ortholog
Description Translation initiation factor eIF-4B; contains an RNA recognition motif and binds to single-stranded RNA; has RNA annealing activity; interacts with Rps20p at the head of the 40S ribosomal subunit and alters the structure of the mRNA entry channel

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.09 0.14 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.05 0 0 0.07 0.07 0.07 0.09 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.94 0.92 0.86 0.8 0.65 0.57 0.56 0.47 0.48 0.43 0.99 0.97 0.98 0.98 0.99 0.99 0.97 0.96 0.9 0.95 0.96 0.91
Endoplasmic Reticulum 0 0 0.07 0 0.1 0.14 0.26 0.29 0.31 0.37 0.31 0.32 0 0 0 0.06 0.06 0 0 0 0.07 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.06 0.06 0.06 0.08 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0 0.05 0.1 0.11 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 6 24 20 29 41 70 93 91 0 0 0 0 0 0 0 0 0 0 0 1
Bud 1 0 0 2 0 2 4 4 2 6 1 3 1 2 1 0 0 0 0 5 1 8 9 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 9 11 20 6 18 44 43 47 72 67 72 4 12 20 14 10 6 0 1 4 0 0 2
Cytoplasm 152 545 500 382 300 485 436 375 358 375 321 299 388 318 448 515 446 291 153 530 513 378 565 468
Endoplasmic Reticulum 0 6 36 18 36 82 174 192 199 301 210 222 7 7 8 30 28 13 1 3 38 2 4 11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2
Golgi 0 0 1 6 2 6 4 38 36 48 56 92 0 1 0 0 0 0 0 0 0 0 0 0
Mitochondria 1 1 0 0 2 29 31 25 34 81 77 91 0 0 0 1 0 1 0 1 2 0 0 2
Nucleus 0 1 0 0 0 0 0 2 0 0 0 0 1 1 0 1 1 0 0 1 0 1 0 1
Nuclear Periphery 0 0 0 0 0 1 0 0 2 0 0 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 4 4 1 1 12 5 6 3 10 1 0 1 3 3 4 0 2 4 6 2 6
Unique Cell Count 153 553 534 413 349 603 668 653 644 804 670 697 393 327 456 527 452 295 159 553 570 400 587 515
Labelled Cell Count 156 563 548 433 357 648 714 720 724 959 828 884 402 341 478 565 488 315 159 553 570 400 587 515


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 47.1 71.0 56.1 47.3 38.2 35.6 33.5 33.1 32.9 32.2 31.4 30.3 67.0 59.8 56.7 80.4 73.8 78.0 63.9 67.7 69.0
Std Deviation (1e-4) 11.2 13.6 9.8 9.1 8.0 6.6 6.4 7.2 5.9 6.0 5.5 6.0 15.0 14.0 12.3 29.2 23.0 25.8 19.0 16.1 19.8
Intensity Change (Log2) -0.24 -0.55 -0.66 -0.74 -0.76 -0.77 -0.8 -0.84 -0.89 0.26 0.09 0.02 0.52 0.4 0.48 0.19 0.27 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 4.7 4.0 4.9 5.9 7.0 9.0 8.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.4 -0.4 1.0 3.7 3.7 4.1 5.1 5.6 5.7 -1.2 1.4 2.1 0.6 0.2 0
Cytoplasm -0.7 -3.8 -6.5 -11.8 -14.1 -14.6 -17.7 -16.9 -18.5 3.8 2.4 3.6 3.3 4.0 3.3
Endoplasmic Reticulum -1.6 1.9 3.8 8.8 9.9 10.3 12.7 10.5 10.7 -3.5 -3.0 -3.8 -0.7 -0.3 -1.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 5.4 5.3 5.5 6.6 8.6 0 0 0 0 0 0
Mitochondria 0 0 5.1 5.0 4.6 5.4 7.6 8.1 8.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 42.6076 65.1223 59.8975 51.3738 51.5682 58.0934 65.9452 78.3424 75.7042 72.4578 61.1164 73.792 72.7364 80.817 78.1951 75.9165 69.9715 75.2632
Actin 0.0068 0.0002 0.0167 0.0244 0.008 0.0041 0.0053 0.0001 0.0002 0 0.0012 0.0005 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002
Bud 0.001 0.0003 0.0009 0.0011 0.001 0.0006 0.0005 0.0002 0.0005 0.0003 0.0017 0.0003 0.0003 0.0003 0.0003 0.0003 0.0002 0.0002
Bud Neck 0.0058 0.0003 0.0006 0.0008 0.0006 0.0016 0.002 0.0004 0.0007 0.0006 0.0005 0.0012 0.0005 0.0005 0.0006 0.001 0.0007 0.0017
Bud Periphery 0.0009 0.0001 0.0007 0.0008 0.0007 0.0004 0.0014 0.0002 0.0004 0.0002 0.0008 0.0005 0.0004 0.0002 0.0003 0.0002 0.0001 0.0003
Bud Site 0.0021 0.0003 0.0019 0.0006 0.0023 0.0002 0.001 0.0002 0.0002 0.0001 0.0007 0.0001 0.0002 0.0003 0.0001 0 0.0001 0
Cell Periphery 0.0026 0.0021 0.0008 0.0014 0.0008 0.0009 0.0169 0.012 0.0104 0.0198 0.0004 0.0044 0.0179 0.0105 0.0167 0.0038 0.0011 0.0065
Cytoplasm 0.7011 0.9587 0.8882 0.7553 0.8353 0.8716 0.7789 0.9329 0.8786 0.7833 0.8417 0.8391 0.7822 0.918 0.8845 0.7964 0.888 0.8878
Cytoplasmic Foci 0.0108 0.0008 0.0068 0.0268 0.011 0.0061 0.0011 0.0007 0.0037 0.0014 0.007 0.0025 0.0008 0.0008 0.001 0.0015 0.0022 0.0022
Eisosomes 0.0002 0 0.0001 0.0002 0.0004 0.0002 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0592 0.0033 0.0041 0.0047 0.0042 0.0414 0.0769 0.0184 0.02 0.0052 0.0055 0.1138 0.094 0.0207 0.0275 0.0573 0.0266 0.063
Endosome 0.018 0.001 0.006 0.0105 0.0174 0.0165 0.0047 0.001 0.0027 0.0059 0.0072 0.0063 0.0031 0.0016 0.0023 0.0078 0.0088 0.0077
Golgi 0.0026 0.0001 0.0013 0.0015 0.0019 0.003 0.0005 0.0001 0.0002 0.0001 0.0031 0.0006 0.0003 0.0004 0.0002 0.0004 0.0003 0.001
Lipid Particles 0.0049 0.0002 0.0013 0.0108 0.0027 0.002 0.0012 0.0015 0.0043 0.0029 0.0014 0.001 0.001 0.0019 0.0017 0.0012 0.0006 0.0007
Mitochondria 0.0047 0.0002 0.0013 0.015 0.0129 0.0039 0.0012 0.0001 0.0002 0.0001 0.0749 0.0007 0.0003 0.0003 0.0001 0.0001 0.0001 0.0005
None 0.0116 0.0003 0.0035 0.0052 0.0066 0.0043 0.0007 0.0001 0.0005 0.0001 0.0047 0.0003 0.0003 0.0001 0.0001 0.0001 0.0003 0.0002
Nuclear Periphery 0.021 0.0014 0.0043 0.0059 0.0101 0.0064 0.0084 0.0014 0.0026 0.0042 0.0026 0.006 0.0075 0.0021 0.0031 0.0049 0.0054 0.003
Nucleolus 0.0067 0.0016 0.0003 0.0039 0.0176 0.0027 0.0014 0.0001 0.0004 0.0002 0.004 0.0001 0.0001 0 0.0001 0 0 0
Nucleus 0.0213 0.0025 0.0023 0.0055 0.0091 0.0029 0.0046 0.0011 0.0019 0.0021 0.0099 0.0018 0.002 0.0013 0.0013 0.0017 0.0018 0.0014
Peroxisomes 0.0084 0 0.0009 0.0037 0.0012 0.0002 0 0 0 0 0.0015 0 0 0 0 0 0 0
Punctate Nuclear 0.0053 0.0001 0.0009 0.0088 0.0015 0.0004 0.0001 0 0.0003 0 0.001 0.0001 0 0 0 0 0.0001 0
Vacuole 0.0864 0.0245 0.0475 0.0893 0.0438 0.0236 0.0843 0.0285 0.0686 0.157 0.0246 0.0182 0.0854 0.0396 0.0573 0.1089 0.0559 0.0212
Vacuole Periphery 0.0183 0.0021 0.0096 0.0238 0.0109 0.0072 0.0087 0.0009 0.0033 0.0166 0.0055 0.0028 0.0034 0.0011 0.0025 0.0142 0.0075 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 352.6518 407.7924 400.3024 281.3631 327.3712 370.1214 294.2144 296.7712 303.124 244.9416
Translational Efficiency 2.3443 2.3487 2.0347 3.1537 2.4031 1.9068 2.0296 2.1467 1.7251 2.1279

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Translation initiation factor eIF-4B; contains an RNA recognition motif and binds to single-stranded RNA; has RNA annealing activity; interacts with Rps20p at the head of the 40S ribosomal subunit and alters the structure of the mRNA entry channel
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Tif3

Tif3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tif3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available