Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of the RNA polymerase II mediator complex; conserved from yeast to human; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 1 | 1 | 6 | 5 | 11 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 1 | – | 2 | 3 | 8 | 7 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 3 | 2 | 3 | 5 | 11 | – | 14 | 6 | 12 | 3 | 8 | 21 | 30 | 50 | 16 | 25 | 20 | 0 | 0 | 0 | 1 | 3 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 2 | 2 | 0 | 2 | 10 | 3 | 6 | 0 | 0 | 0 | 0 | 2 | 1 |
Endosome | 1 | 0 | 1 | 0 | 1 | 3 | – | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 11 | 9 | 8 | 6 | 0 | 0 | 1 | 6 | 11 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 2 | 0 | 3 | 2 | 0 | 0 | 1 | 0 |
Mitochondria | 5 | 1 | 16 | 14 | 3 | 29 | – | 47 | 56 | 52 | 85 | 74 | 1 | 2 | 1 | 10 | 16 | 3 | 2 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 453 | 118 | 96 | 46 | 61 | 136 | – | 164 | 97 | 121 | 158 | 150 | 171 | 176 | 187 | 111 | 86 | 82 | 458 | 107 | 86 | 133 | 181 | 187 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 90 | 33 | 18 | 18 | 9 | 63 | – | 88 | 69 | 63 | 89 | 68 | 46 | 42 | 58 | 29 | 31 | 20 | 91 | 45 | 25 | 66 | 97 | 93 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 7 | 2 | 0 | 10 | 6 | 6 |
Vac/Vac Membrane | 27 | 7 | 3 | 2 | 10 | 10 | – | 6 | 5 | 3 | 0 | 6 | 13 | 6 | 13 | 49 | 32 | 26 | 19 | 3 | 3 | 10 | 16 | 26 |
Unique Cell Count | 518 | 138 | 110 | 72 | 82 | 213 | 257 | 174 | 188 | 241 | 231 | 228 | 226 | 281 | 206 | 176 | 150 | 600 | 164 | 119 | 234 | 328 | 348 | |
Labelled Cell Count | 582 | 162 | 137 | 84 | 89 | 255 | 326 | 237 | 265 | 344 | 319 | 258 | 258 | 315 | 239 | 207 | 166 | 600 | 164 | 119 | 234 | 328 | 348 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 11.4 | 15.2 | 7.9 | 10.4 | 11.4 | 8.5 | – | 7.8 | 6.9 | 7.6 | 7.0 | 7.1 | 11.9 | 11.7 | 11.4 | 14.3 | 13.1 | 13.2 | 10.9 | 11.9 | 12.3 |
Std Deviation (1e-4) | 2.1 | 2.7 | 1.8 | 2.2 | 2.8 | 1.9 | – | 1.7 | 1.9 | 2.2 | 1.6 | 1.9 | 2.6 | 2.4 | 2.7 | 4.6 | 4.2 | 3.8 | 2.2 | 2.7 | 2.8 |
Intensity Change (Log2) | – | – | – | 0.39 | 0.53 | 0.1 | – | -0.02 | -0.19 | -0.06 | -0.18 | -0.15 | 0.59 | 0.56 | 0.53 | 0.86 | 0.73 | 0.74 | 0.47 | 0.59 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 1.8 | 0 | 0 | 2.5 | 3.4 | 4.2 | 2.2 | 3.5 | 3.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.9 | -2.5 | -0.2 | – | 0.9 | 3.3 | 2.6 | 4.0 | 3.4 | -5.6 | -5.2 | 0 | -3.0 | -1.4 | -3.8 |
Nucleus | -3.7 | -2.3 | -4.4 | – | -4.5 | -5.5 | -4.3 | -4.2 | -4.3 | -2.6 | -2.1 | -4.1 | -5.9 | -6.6 | -5.6 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.4 | -1.1 | 2.6 | – | 3.5 | 4.1 | 3.2 | 3.9 | 2.6 | 0.8 | 0.5 | 1.0 | -0.5 | 0.3 | -0.7 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.2 | 0 | 0 | 4.8 | 3.9 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.6127 | 7.7737 | 7.1502 | 6.4152 | 4.8767 | 6.8009 | 5.6265 | 7.5467 | 6.6669 | 6.743 | 5.0205 | 6.8153 | 7.5007 | 8.8287 | 8.5934 | 8.2078 | 7.7071 | 8.2277 |
Actin | 0.0075 | 0 | 0.0008 | 0.0007 | 0.0361 | 0.0088 | 0.0104 | 0 | 0.0134 | 0 | 0.0065 | 0 | 0.0065 | 0 | 0.0014 | 0.0021 | 0.0009 | 0.0051 |
Bud | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0005 | 0 | 0.0005 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0016 | 0.0017 | 0.0022 | 0.0002 | 0.0009 | 0.0004 | 0.0011 | 0.0015 | 0.0009 | 0.0003 | 0.0001 | 0.0002 | 0.0008 | 0.0028 |
Bud Periphery | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.001 | 0 | 0.0009 | 0 | 0.0009 | 0.0002 | 0.0011 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0004 | 0.0002 | 0.0003 | 0.0008 | 0.0013 | 0.0002 | 0.0028 | 0.0001 | 0.0023 | 0.0001 | 0.0019 | 0.0002 | 0.0014 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0004 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0038 | 0.0002 | 0.0099 | 0.0084 | 0.0023 | 0.0008 | 0.0131 | 0.0005 | 0.0033 | 0.0005 | 0.0026 | 0.0002 | 0.0075 | 0.0034 | 0.0017 | 0.0005 | 0.0024 | 0.0027 |
Cytoplasmic Foci | 0.0065 | 0 | 0.0007 | 0.0141 | 0.0136 | 0.0016 | 0.0326 | 0 | 0.0101 | 0.017 | 0.0394 | 0 | 0.0152 | 0 | 0.0004 | 0.0132 | 0.0029 | 0.0005 |
Eisosomes | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0021 | 0 | 0.0001 | 0.0028 | 0.0021 | 0.0008 | 0.006 | 0 | 0.0041 | 0.0001 | 0.002 | 0 | 0.002 | 0 | 0.0008 | 0.0001 | 0.002 | 0.0027 |
Endosome | 0.0085 | 0 | 0.0004 | 0.0181 | 0.0217 | 0.0026 | 0.0282 | 0 | 0.0141 | 0.0081 | 0.049 | 0.0001 | 0.0224 | 0 | 0.0021 | 0.0045 | 0.013 | 0.0032 |
Golgi | 0.0021 | 0 | 0.0005 | 0.0015 | 0.0243 | 0.0012 | 0.0036 | 0 | 0.004 | 0.0025 | 0.0334 | 0 | 0.0034 | 0 | 0.0002 | 0.0047 | 0.0008 | 0.0009 |
Lipid Particles | 0.0026 | 0 | 0.0012 | 0.0038 | 0.0539 | 0.0047 | 0.0156 | 0 | 0.0063 | 0.0246 | 0.0288 | 0 | 0.009 | 0 | 0.0001 | 0.0052 | 0.0044 | 0.0004 |
Mitochondria | 0.0016 | 0.0001 | 0.0025 | 0.0004 | 0.0144 | 0.0005 | 0.0023 | 0.0001 | 0.0035 | 0.0004 | 0.029 | 0.0001 | 0.0049 | 0.0001 | 0.0004 | 0.0007 | 0.0003 | 0.0005 |
None | 0.0014 | 0 | 0.0001 | 0.002 | 0.0168 | 0.0005 | 0.0064 | 0 | 0.0006 | 0 | 0.0082 | 0 | 0.0026 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0002 |
Nuclear Periphery | 0.0122 | 0.0031 | 0.0028 | 0.0043 | 0.0424 | 0.0025 | 0.0238 | 0.0012 | 0.009 | 0.001 | 0.0377 | 0.0006 | 0.0368 | 0.002 | 0.0036 | 0.0012 | 0.0181 | 0.0111 |
Nucleolus | 0.0096 | 0.0232 | 0.0151 | 0.0051 | 0.0196 | 0.025 | 0.0177 | 0.0086 | 0.0083 | 0.0052 | 0.0287 | 0.0257 | 0.0102 | 0.0089 | 0.0066 | 0.0061 | 0.0109 | 0.0367 |
Nucleus | 0.9244 | 0.9702 | 0.9604 | 0.9198 | 0.6838 | 0.9421 | 0.7809 | 0.9878 | 0.9023 | 0.9372 | 0.6809 | 0.9695 | 0.8459 | 0.9732 | 0.9805 | 0.9477 | 0.9215 | 0.9273 |
Peroxisomes | 0.0031 | 0 | 0.0029 | 0.0005 | 0.0135 | 0.0005 | 0.0106 | 0 | 0.0063 | 0.0008 | 0.0353 | 0 | 0.0063 | 0 | 0.0001 | 0.0102 | 0.0004 | 0.0001 |
Punctate Nuclear | 0.0106 | 0.0025 | 0.0016 | 0.0151 | 0.0469 | 0.0056 | 0.0317 | 0.0014 | 0.0037 | 0.001 | 0.0054 | 0.0013 | 0.0192 | 0.0113 | 0.0013 | 0.003 | 0.0195 | 0.0046 |
Vacuole | 0.0016 | 0.0001 | 0.0001 | 0.0015 | 0.0014 | 0.0004 | 0.0084 | 0.0001 | 0.0048 | 0.0007 | 0.0038 | 0.0002 | 0.0019 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0008 |
Vacuole Periphery | 0.0008 | 0 | 0.0001 | 0.0004 | 0.0026 | 0.0002 | 0.0017 | 0 | 0.0009 | 0.0002 | 0.0044 | 0 | 0.0017 | 0 | 0.0001 | 0 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.1311 | 12.4858 | 13.8975 | 15.999 | 19.6181 | 9.3063 | 13.6764 | 13.4909 | 14.8256 | 14.7275 |
Translational Efficiency | 1.321 | 1.3984 | 1.5687 | 0.9811 | 0.8014 | 2.6177 | 1.5344 | 1.3758 | 1.6457 | 1.3197 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2459 | 1159 | 432 | 1262 | 1513 | 2078 | 97 | 1623 | 3972 | 3237 | 529 | 2885 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1000.56 | 1004.11 | 1606.89 | 1616.41 | 1007.41 | 1149.50 | 1658.81 | 1615.42 | 1003.17 | 1097.44 | 1616.41 | 1615.85 |
Standard Deviation | 178.85 | 221.70 | 224.97 | 284.19 | 173.49 | 186.94 | 306.63 | 276.82 | 176.86 | 211.87 | 242.85 | 280.07 |
Intensity Change Log 2 | — | 0.005110 | 0.683463 | 0.691985 | — | 0.190356 | 0.719498 | 0.681258 | — | 0.101019 | 0.701655 | 0.686614 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000338 | 0.001387 | 0.001325 | 0.002167 | 0.000743 | 0.000939 | 0.003551 | 0.002925 | 0.000492 | 0.001100 | 0.001733 | 0.002593 |
Bud Neck | 0.016051 | 0.025130 | 0.027081 | 0.040841 | 0.019298 | 0.032745 | 0.022439 | 0.034968 | 0.017288 | 0.030018 | 0.026230 | 0.037537 |
Bud Site | 0.000778 | 0.003390 | 0.002515 | 0.018536 | 0.001392 | 0.003146 | 0.006799 | 0.019937 | 0.001012 | 0.003233 | 0.003300 | 0.019324 |
Cell Periphery | 0.000053 | 0.000206 | 0.000111 | 0.000329 | 0.000105 | 0.000100 | 0.000293 | 0.000185 | 0.000073 | 0.000138 | 0.000144 | 0.000248 |
Cytoplasm | 0.001933 | 0.013674 | 0.001584 | 0.032689 | 0.001239 | 0.003385 | 0.003194 | 0.033564 | 0.001668 | 0.007069 | 0.001879 | 0.033181 |
Cytoplasmic Foci | 0.000049 | 0.000736 | 0.000144 | 0.013906 | 0.000134 | 0.000390 | 0.006245 | 0.005167 | 0.000081 | 0.000514 | 0.001263 | 0.008989 |
Eisosomes | 0.000030 | 0.000030 | 0.000044 | 0.000029 | 0.000030 | 0.000034 | 0.000087 | 0.000028 | 0.000030 | 0.000032 | 0.000052 | 0.000028 |
Endoplasmic Reticulum | 0.002090 | 0.003860 | 0.001756 | 0.004819 | 0.003414 | 0.003521 | 0.041939 | 0.003391 | 0.002594 | 0.003642 | 0.009125 | 0.004015 |
Endosome | 0.000213 | 0.000643 | 0.000124 | 0.013074 | 0.000323 | 0.000794 | 0.004706 | 0.013451 | 0.000255 | 0.000740 | 0.000964 | 0.013286 |
Golgi | 0.000133 | 0.000558 | 0.000226 | 0.021853 | 0.000194 | 0.000417 | 0.001735 | 0.006751 | 0.000156 | 0.000467 | 0.000502 | 0.013357 |
Lipid Particles | 0.000040 | 0.000293 | 0.000104 | 0.000776 | 0.000129 | 0.000722 | 0.008729 | 0.000818 | 0.000074 | 0.000568 | 0.001686 | 0.000800 |
Mitochondria | 0.000846 | 0.001837 | 0.002060 | 0.009180 | 0.001246 | 0.002352 | 0.006538 | 0.010790 | 0.000998 | 0.002167 | 0.002881 | 0.010086 |
Mitotic Spindle | 0.002570 | 0.004367 | 0.002957 | 0.150515* | 0.004089 | 0.009103 | 0.022543 | 0.173879* | 0.003149 | 0.007407 | 0.006548 | 0.163659* |
None | 0.001792 | 0.008236 | 0.004738 | 0.007854 | 0.002442 | 0.003171 | 0.002624 | 0.010209 | 0.002040 | 0.004984 | 0.004350 | 0.009179 |
Nuclear Periphery | 0.000520 | 0.000554 | 0.000422 | 0.003263 | 0.000597 | 0.000694 | 0.002215 | 0.005306 | 0.000549 | 0.000644 | 0.000751 | 0.004412 |
Nuclear Periphery Foci | 0.000214 | 0.001924 | 0.000095 | 0.002233 | 0.000511 | 0.000578 | 0.008250 | 0.004655 | 0.000327 | 0.001060 | 0.001590 | 0.003596 |
Nucleolus | 0.089958 | 0.049754 | 0.027769 | 0.006386 | 0.092301 | 0.085961 | 0.011560 | 0.006861 | 0.090851 | 0.072997 | 0.024797 | 0.006654 |
Nucleus | 0.880934* | 0.880466* | 0.924117* | 0.626351* | 0.870348* | 0.849662* | 0.833346* | 0.639332* | 0.876901* | 0.860691* | 0.907472* | 0.633653* |
Peroxisomes | 0.000252 | 0.000818 | 0.001283 | 0.001081 | 0.000587 | 0.000726 | 0.001552 | 0.000882 | 0.000380 | 0.000759 | 0.001332 | 0.000969 |
Vacuole | 0.000428 | 0.001703 | 0.001369 | 0.025941 | 0.000585 | 0.000933 | 0.007758 | 0.016408 | 0.000488 | 0.001208 | 0.002541 | 0.020578 |
Vacuole Periphery | 0.000779 | 0.000435 | 0.000175 | 0.018178 | 0.000293 | 0.000629 | 0.003897 | 0.010493 | 0.000594 | 0.000560 | 0.000858 | 0.013855 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -14.26 | -14.89 | -13.96 | -2.21 | 1.42 | -4.15 | -9.24 | -4.74 | -2.94 | 6.08 | -14.82 | -16.04 | -11.42 | -3.98 | 4.32 |
Bud Neck | -8.40 | -10.67 | -15.42 | -8.36 | -3.65 | -9.28 | -4.91 | -11.96 | -4.41 | -0.54 | -14.15 | -11.25 | -20.14 | -8.58 | -3.17 |
Bud Site | -9.14 | -11.51 | -11.55 | -7.68 | -6.59 | -4.11 | -6.37 | -10.19 | -7.45 | -0.33 | -8.25 | -12.26 | -15.48 | -10.58 | -6.79 |
Cell Periphery | -6.75 | -6.99 | -15.82 | -3.26 | -9.06 | 0.12 | -4.56 | -2.99 | -5.22 | 3.34 | -3.25 | -5.87 | -10.87 | -6.49 | -4.05 |
Cytoplasm | -5.77 | 0.36 | -12.43 | -2.98 | -13.25 | -3.53 | -2.32 | -14.46 | -11.55 | -13.65 | -6.24 | -0.29 | -18.51 | -10.73 | -19.04 |
Cytoplasmic Foci | -3.40 | -5.03 | -4.30 | -2.54 | -3.94 | -1.84 | -1.93 | -5.12 | -3.74 | 0.57 | -4.14 | -2.78 | -6.18 | -4.10 | -4.07 |
Eisosomes | -1.88 | -10.00 | -7.42 | -5.30 | 5.53 | -2.78 | -8.27 | -3.67 | -1.40 | 7.66 | -3.99 | -12.28 | -7.77 | -4.30 | 9.29 |
Endoplasmic Reticulum | -10.38 | -2.86 | -9.86 | 0.28 | -5.38 | -0.54 | -1.25 | 1.53 | 2.14 | 1.32 | -8.53 | -1.36 | -6.36 | 1.67 | 0.41 |
Endosome | -3.18 | -0.25 | -4.77 | -3.42 | -4.75 | -2.15 | -1.44 | -3.97 | -2.80 | -1.29 | -3.62 | -1.15 | -6.14 | -4.23 | -5.44 |
Golgi | -2.90 | -7.26 | -3.07 | -2.50 | -2.73 | -2.68 | -3.07 | -3.45 | -2.62 | -0.86 | -4.45 | -6.74 | -4.13 | -3.30 | -3.30 |
Lipid Particles | -2.60 | -5.65 | -8.87 | -5.05 | -7.23 | -1.36 | -1.94 | -8.01 | -0.15 | 1.44 | -1.89 | -2.48 | -12.50 | -1.46 | -1.37 |
Mitochondria | -6.10 | -13.61 | -20.47 | -15.87 | -11.60 | -2.92 | -7.93 | -7.79 | -5.17 | -0.48 | -4.98 | -15.63 | -15.02 | -9.90 | -6.89 |
Mitotic Spindle | -2.09 | -2.46 | -15.70 | -15.11 | -13.97 | -2.43 | -1.33 | -16.22 | -15.25 | -8.92 | -3.87 | -2.56 | -22.78 | -21.29 | -18.84 |
None | -8.07 | -4.21 | -13.90 | -1.48 | -6.01 | -1.45 | -1.58 | -13.50 | -11.95 | -10.70 | -6.92 | -4.24 | -19.33 | -10.70 | -9.71 |
Nuclear Periphery | -1.28 | -3.47 | -17.99 | -17.74 | -17.08 | -1.22 | -3.87 | -14.62 | -14.16 | -6.01 | -2.26 | -4.94 | -22.92 | -22.17 | -19.60 |
Nuclear Periphery Foci | -3.80 | 1.50 | -6.92 | 1.20 | -7.38 | -0.37 | -0.96 | -2.53 | -2.51 | 0.30 | -4.22 | -0.15 | -6.44 | -0.70 | -4.15 |
Nucleolus | 9.98 | 10.13 | 28.81* | 13.93 | 7.64 | 0.42 | 15.61 | 24.71* | 26.90* | 2.44 | 4.66 | 13.73 | 38.53* | 29.86* | 8.23 |
Nucleus | 2.06 | 0.16 | 17.89 | 15.30 | 15.16 | 4.84 | 0.66 | 13.78 | 10.48 | 5.12 | 6.63 | 0.01 | 22.83 | 17.61 | 16.66 |
Peroxisomes | -4.56 | -6.96 | -3.49 | 1.99 | 4.94 | -1.15 | -3.90 | 1.71 | 3.54 | 5.17 | -5.17 | -7.10 | -1.40 | 3.83 | 6.46 |
Vacuole | -3.21 | -6.37 | -12.64 | -11.65 | -11.11 | -1.52 | -2.91 | -12.02 | -11.82 | 0.15 | -4.15 | -4.47 | -17.48 | -16.63 | -9.11 |
Vacuole Periphery | 0.98 | 1.63 | -1.77 | -2.34 | -2.62 | -1.70 | -1.05 | -2.04 | -1.43 | -0.74 | 0.27 | 1.11 | -2.36 | -2.67 | -3.10 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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