Standard name
Human Ortholog
Description Adapter that links synaptojanins to the cortical actin cytoskeleton; the synaptojanins are Inp52p and Inp53p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.43 0.23 0.55 0.57 0.55 0.31 0.24 – 0.18 0.23 0.13 0.18 0.57 0.55 0.53 0.37 0.43 0.39 0.65 0.5 0.52 0.62 0.47 0.58
Bud 0.14 0.15 0.08 0.19 0.24 0.1 0.16 – 0.11 0.16 0.14 0.09 0.17 0.13 0.09 0.12 0.11 0.11 0.05 0 0.05 0 0.1 0.1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.07 0
Bud Site 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.11 0 0 0 0 – 0 0 0 0.06 0.09 0.06 0.13 0.1 0.08 0.09 0 0 0 0 0 0
Cytoplasm 0.12 0.08 0.17 0.13 0.16 0.14 0.14 – 0.13 0.1 0.09 0.13 0.19 0.21 0.21 0.18 0.22 0.23 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.08 0.09 0.07 0.08 0.06 – 0 0 0.07 0.08 0.11 0 0.06 0.05 0 0.06 0 0 0 0 0 0
Endosome 0.05 0.07 0 0 0 0.06 0.11 – 0 0.07 0 0 0 0.06 0 0.09 0.07 0 0 0.14 0.06 0 0.15 0.1
Golgi 0.13 0.07 0.28 0.07 0.12 0.1 0.05 – 0.06 0 0 0.06 0.21 0.23 0.31 0.08 0.11 0.14 0.06 0.05 0.06 0.05 0 0
Mitochondria 0.57 0.59 0.12 0.27 0.09 0.39 0.45 – 0.72 0.58 0.8 0.69 0.08 0 0 0.09 0.05 0.06 0 0.11 0.07 0 0.06 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.21 0.06 0.11 0.18 0.23 0.21 0.14 – 0.07 0.08 0 0 0.11 0.22 0.11 0.1 0.06 0.12 0.08 0.06 0.12 0.07 0.06 0
SpindlePole 0 0 0 0 0.11 0.06 0.08 – 0 0.1 0 0 0 0.05 0 0.24 0.15 0.22 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 122 31 86 77 123 59 69 – 42 51 31 46 30 87 89 125 138 132 209 111 113 36 61 98
Bud 40 20 13 26 54 19 45 – 26 35 34 22 9 21 15 39 34 36 15 5 10 2 12 17
Bud Neck 0 0 1 1 1 0 1 – 0 2 0 0 0 0 0 3 6 1 5 3 11 4 9 4
Bud Site 20 2 1 6 8 4 12 – 8 9 3 6 2 2 0 1 8 1 – – – – – –
Cell Periphery 2 3 17 4 9 1 5 – 0 5 2 16 5 10 21 35 24 32 0 1 0 0 0 0
Cytoplasm 34 11 26 18 35 27 41 – 30 22 21 32 10 33 35 62 70 79 11 5 9 0 3 2
Endoplasmic Reticulum 5 5 12 12 15 15 18 – 8 7 18 21 6 7 10 16 12 20 2 1 1 0 1 1
Endosome 14 9 6 2 9 12 33 – 6 15 1 7 2 9 5 31 23 13 14 31 12 2 19 16
Golgi 36 10 43 10 27 19 13 – 14 3 3 14 11 36 52 28 36 47 19 10 13 3 1 5
Mitochondria 164 79 19 37 20 76 130 – 171 126 194 176 4 7 6 30 17 22 8 23 14 2 7 10
Nucleus 2 0 0 0 1 4 3 – 4 1 0 1 0 1 1 2 4 3 0 0 0 0 0 0
Nuclear Periphery 2 1 0 0 1 1 1 – 0 0 0 5 1 0 0 0 0 0 1 3 0 0 0 0
Nucleolus 1 4 0 1 1 0 3 – 1 2 2 3 0 0 0 3 1 0 0 0 0 0 0 0
Peroxisomes 59 8 17 25 51 41 41 – 17 17 8 11 6 35 19 35 20 40 27 12 25 4 7 7
SpindlePole 7 2 6 2 25 12 23 – 10 22 7 4 1 8 5 80 49 76 4 4 1 0 1 1
Vac/Vac Membrane 3 11 8 1 4 1 4 – 0 0 1 2 0 0 0 17 10 11 0 3 1 0 1 1
Unique Cell Count 287 135 156 136 224 193 287 237 218 242 254 53 158 167 337 318 342 322 221 216 58 132 169
Labelled Cell Count 511 196 255 222 384 291 442 337 317 325 366 87 256 258 507 452 513 322 221 216 58 132 169


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 8.7 8.6 7.7 8.0 6.7 6.5 – 5.7 5.8 5.1 5.6 8.9 9.2 8.5 9.2 8.7 8.7 7.5 8.8 8.8
Std Deviation (1e-4) 1.5 2.0 1.7 1.3 1.9 1.4 1.4 – 1.2 1.4 1.2 1.3 1.5 1.6 1.6 5.5 1.4 1.4 0.9 1.4 1.5
Intensity Change (Log2) – – – -0.16 -0.11 -0.36 -0.4 – -0.59 -0.56 -0.75 -0.62 0.05 0.1 -0.01 0.1 0.02 0.02 -0.2 0.04 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 0 -4.6 -6.6 – -7.7 -6.3 -9.0 -7.8 0.2 0 -0.3 -3.8 -2.4 -3.4
Bud 2.7 4.0 0.5 2.2 – 0.9 2.2 1.7 0.1 1.8 1.4 0.2 1.1 0.8 0.8
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.6 -2.6 -4.4 -4.2 – -5.2 -3.5 -4.6 -1.7 -0.3 -1.4 0.5 -0.2 -1.2 -0.5
Cytoplasm -0.8 -0.3 -0.7 -0.7 – -1.1 -1.9 -2.4 -1.1 0.4 1.0 1.0 0.5 1.4 1.6
Endoplasmic Reticulum 0.4 -0.4 0 -0.6 – -1.9 -1.9 -0.1 0.2 0 -1.2 -0.6 -1.3 -1.8 -0.8
Endosome 0 0.1 1.0 2.7 – 0 1.3 0 0 0 0.8 -0.4 2.1 1.4 0
Golgi -4.5 -3.8 -4.3 -7.0 – -6.0 -7.6 -8.0 -6.3 -1.0 -1.0 0.7 -5.7 -4.5 -3.7
Mitochondria 3.3 -1.0 5.7 7.0 – 11.6 8.9 13.3 11.2 -0.9 -2.5 -2.9 -1.1 -2.6 -2.2
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.8 3.0 2.6 1.0 – -1.3 -1.0 -3.0 -2.6 0.1 2.7 0.1 -0.2 -1.8 0.3
SpindlePole 0 2.6 1.0 1.7 – 0.2 2.3 -0.5 0 0 0.5 -0.4 5.4 3.7 5.1
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 -1.1 -1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9187 2.8324 2.8842 2.5431 2.4146 3.026 4.0785 3.2078 3.3662 3.2795 3.3239 3.5107 4.0564 3.0539 3.191 3.1134 3.1737 3.2548
Actin 0.6503 0.531 0.7404 0.6937 0.839 0.7566 0.7123 0.631 0.7944 0.7222 0.8727 0.7606 0.7554 0.6827 0.7841 0.6553 0.6342 0.7467
Bud 0.0015 0.0123 0.0321 0.0221 0.0055 0.0019 0.0022 0.011 0.0186 0.0254 0.0135 0.0019 0.0017 0.014 0.0395 0.0522 0.0094 0.0012
Bud Neck 0.0046 0.0024 0.0017 0.0008 0.0064 0.0771 0.0037 0.0031 0.0056 0.0013 0.0152 0.085 0.0042 0.0061 0.0058 0.0023 0.0059 0.1184
Bud Periphery 0.0017 0.0127 0.0531 0.0664 0.0128 0.003 0.0034 0.0142 0.0248 0.0539 0.0291 0.0029 0.0017 0.0091 0.0444 0.0705 0.0081 0.002
Bud Site 0.0131 0.1238 0.0258 0.0058 0.0052 0.0026 0.0153 0.1173 0.0322 0.0079 0.0058 0.0038 0.0162 0.0931 0.0244 0.0489 0.0328 0.0035
Cell Periphery 0.0003 0.0009 0.0011 0.0005 0.0008 0.0002 0.0003 0.0017 0.0004 0.0005 0.0004 0.0003 0.0004 0.0008 0.0005 0.0015 0.0008 0.0004
Cytoplasm 0.0044 0.0612 0.0177 0.0068 0.0193 0.0036 0.0051 0.0377 0.0037 0.003 0.0013 0.0065 0.0125 0.0497 0.0205 0.0097 0.0367 0.0037
Cytoplasmic Foci 0.0603 0.078 0.0241 0.0355 0.0104 0.0269 0.0421 0.0393 0.02 0.0152 0.0177 0.0291 0.0401 0.0529 0.021 0.0296 0.0666 0.0344
Eisosomes 0.0006 0.0004 0.0006 0.0005 0.0005 0.0007 0.0007 0.0013 0.0004 0.0003 0.0004 0.0006 0.0018 0.0005 0.0003 0.001 0.0005 0.0006
Endoplasmic Reticulum 0.0014 0.0008 0.0005 0.0022 0.0002 0.0002 0.0011 0.0011 0.0005 0.0004 0.0011 0.0002 0.0034 0.0007 0.0004 0.0025 0.0045 0.001
Endosome 0.0362 0.02 0.0055 0.0299 0.0073 0.0145 0.0268 0.0074 0.0081 0.0222 0.0116 0.0052 0.0192 0.012 0.0034 0.0511 0.0584 0.0069
Golgi 0.049 0.0345 0.0143 0.0412 0.0215 0.0342 0.0631 0.0334 0.0127 0.0326 0.0049 0.016 0.0247 0.0121 0.0101 0.0147 0.0552 0.0126
Lipid Particles 0.0199 0.0027 0.0039 0.0124 0.01 0.0034 0.0067 0.0019 0.0021 0.0016 0.0039 0.004 0.0098 0.0013 0.0017 0.0029 0.004 0.005
Mitochondria 0.0154 0.0104 0.0167 0.0219 0.0209 0.0109 0.0133 0.0051 0.0061 0.022 0.0039 0.0053 0.0172 0.0037 0.0086 0.0113 0.0094 0.0031
None 0.0029 0.0176 0.0035 0.0096 0.0025 0.005 0.0279 0.0327 0.0106 0.0004 0.0006 0.006 0.0256 0.0043 0.0068 0.0024 0.0195 0.0121
Nuclear Periphery 0.0011 0.0003 0.0002 0.0031 0.0005 0.0013 0.0028 0.0005 0.0004 0.0002 0.0039 0.0009 0.0174 0.0003 0.0002 0.0011 0.0024 0.0014
Nucleolus 0.003 0.0002 0.0001 0.0003 0.0001 0.0001 0.0005 0.0005 0.0003 0.0001 0.0006 0.0002 0.0016 0.0003 0.0001 0.0006 0.0013 0.0001
Nucleus 0.002 0.0005 0.0002 0.0004 0.0001 0.0002 0.0008 0.0011 0.0003 0.0001 0.0016 0.0003 0.0079 0.0012 0.0002 0.0005 0.0044 0.0002
Peroxisomes 0.1228 0.0874 0.0567 0.0429 0.0341 0.056 0.0679 0.0558 0.0563 0.0891 0.0074 0.0656 0.0318 0.053 0.027 0.0355 0.0351 0.0449
Punctate Nuclear 0.0071 0.0011 0.001 0.0016 0.0027 0.0008 0.0026 0.0028 0.0014 0.0002 0.0028 0.0053 0.0044 0.0005 0.0003 0.0007 0.0071 0.0004
Vacuole 0.0019 0.0015 0.0005 0.0014 0.0002 0.0004 0.0008 0.0008 0.0007 0.0009 0.001 0.0001 0.0013 0.0015 0.0005 0.0047 0.0031 0.0007
Vacuole Periphery 0.0005 0.0004 0.0002 0.0011 0.0002 0.0002 0.0007 0.0002 0.0002 0.0006 0.0006 0.0001 0.0017 0.0003 0.0002 0.0011 0.0008 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.199 10.8268 20.2963 17.9249 19.0087 20.3867 19.5893 18.0823 17.4567 20.3336
Translational Efficiency 1.7678 1.6791 0.8934 1.1317 1.0416 0.9628 1.0148 1.2834 1.1378 0.9514

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Adapter that links synaptojanins to the cortical actin cytoskeleton; the synaptojanins are Inp52p and Inp53p
Localization
Cell Percentages actin (69%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bsp1

Bsp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bsp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available