ORF
Human Ortholog
Description Protein of unknown function; predicted to encode a pyridoxal 5'-phosphate synthase based on sequence similarity but purified protein does not possess this activity, nor does it bind flavin mononucleotide (FMN); transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.98 0.95 0.9 0.87 – 0.76 0.77 0.71 0.7 1.0 0.99 0.99 0.95 0.96 0.95 0.91 0.92 0.93 0.91 0.91 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0 – 0.21 0.19 0.26 0.24 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.11 – 0.13 0.08 0.13 0.11 0 0 0 0 0.07 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 3 1 2 0 2 2
Bud 0 0 0 0 0 3 1 – 4 2 3 4 0 0 1 0 0 0 6 1 0 5 8 10
Bud Neck 0 0 2 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 – 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 1 2 3 4 2 6 – 7 13 9 9 0 2 1 3 2 0 0 0 1 0 0 0
Cytoplasm 406 200 250 288 304 284 461 – 260 290 273 292 208 355 333 228 162 104 383 196 237 164 281 277
Endoplasmic Reticulum 2 1 0 0 2 1 0 – 1 5 0 12 0 0 0 7 1 5 0 0 1 0 2 1
Endosome 1 0 0 0 3 1 3 – 1 2 2 1 0 0 0 0 0 0 6 1 0 0 2 3
Golgi 2 0 1 2 0 0 0 – 0 0 0 0 0 1 0 1 0 0 3 1 0 1 1 1
Mitochondria 7 0 0 4 2 47 20 – 73 72 102 102 0 2 0 3 2 1 2 1 5 1 2 4
Nucleus 0 4 3 1 0 1 8 – 3 8 7 12 1 1 0 4 1 2 1 0 1 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 0 1 0 1 0 0 0 0 1 0 1 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 – 0 2 0 0 0 0 1 0 1 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 3 – 1 3 2 2 0 0 0 0 0 0 4 0 0 0 1 4
Vac/Vac Membrane 9 1 4 3 12 8 56 – 45 30 50 44 0 2 3 9 11 9 1 4 0 1 2 3
Unique Cell Count 415 204 254 294 320 316 530 343 378 387 418 208 360 336 240 168 109 422 213 256 181 308 313
Labelled Cell Count 433 207 262 303 327 347 558 395 430 449 481 209 363 339 255 181 121 422 213 256 181 308 313


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.7 6.5 6.1 7.4 5.1 6.8 – 6.3 6.6 6.4 6.6 6.4 6.6 7.6 13.4 12.9 13.3 5.9 6.6 6.2
Std Deviation (1e-4) 2.1 1.3 1.8 1.8 1.8 1.5 2.0 – 2.1 2.1 2.4 2.3 1.8 1.9 2.4 4.2 4.7 3.9 3.1 2.9 3.3
Intensity Change (Log2) – – – -0.08 0.19 -0.35 0.07 – -0.03 0.03 -0.01 0.04 -0.01 0.04 0.25 1.05 1.0 1.04 -0.12 0.03 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 2.1 0 0 0 0 0 0 0 0
Cytoplasm -0.4 -2.2 -4.2 -5.2 – -7.7 -7.6 -8.9 -9.1 1.8 0.2 0.8 -2.1 -1.3 -1.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.4 3.1 – 7.8 7.4 8.9 8.5 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 1.4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.6 0.8 4.4 – 5.1 3.5 5.1 4.4 0 0 0 1.5 2.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6718 3.2626 2.8827 2.1575 2.3117 2.8988 3.8094 5.1085 4.3873 3.0259 4.2388 4.5251 6.7201 7.9308 7.0298 7.6258 6.251 7.3097
Actin 0.0327 0.0004 0.0092 0.0012 0.0013 0.0108 0.0098 0.0004 0.0013 0.0077 0.0015 0.0097 0.0015 0.0003 0.0014 0.0001 0.0083 0.0004
Bud 0.0003 0.0001 0.0039 0.0003 0.0001 0.0001 0.0007 0.0031 0.0004 0.0006 0.0008 0.0002 0.0001 0.0001 0.0002 0.0001 0.0004 0.0001
Bud Neck 0.0008 0.0001 0.0005 0.0002 0.0003 0.0007 0.0008 0.0002 0.0004 0.0006 0.0011 0.001 0.0001 0.0001 0.0002 0.0003 0.0007 0.0009
Bud Periphery 0.0003 0.0001 0.0029 0.0008 0 0.0001 0.0006 0.0007 0.0005 0.0006 0.0005 0.0001 0 0 0.0001 0 0.0003 0
Bud Site 0.0017 0.0004 0.004 0.0001 0 0.0001 0.0013 0.003 0.0019 0.0008 0.0005 0.0001 0.0005 0.0003 0.0006 0.0001 0.001 0
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.7351 0.8942 0.8853 0.8951 0.8296 0.8757 0.8727 0.9341 0.9009 0.7925 0.8414 0.9308 0.8006 0.8634 0.8427 0.936 0.6789 0.9705
Cytoplasmic Foci 0.0273 0.0033 0.0111 0.005 0.0066 0.0058 0.0097 0.0035 0.0092 0.0203 0.0255 0.0084 0.0111 0.0014 0.0089 0.0027 0.0435 0.0036
Eisosomes 0.0002 0 0 0.0001 0 0.0001 0.0001 0 0 0 0 0.0001 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0061 0.0016 0.0043 0.0034 0.0055 0.0021 0.003 0.0011 0.0017 0.0024 0.0052 0.0017 0.003 0.0024 0.0024 0.0006 0.0014 0.0012
Endosome 0.0259 0.0013 0.0054 0.0017 0.0039 0.0063 0.0128 0.0041 0.0048 0.0063 0.0332 0.0058 0.0108 0.0003 0.0018 0.001 0.0708 0.0011
Golgi 0.0075 0.0001 0.0011 0.0002 0.0002 0.005 0.0012 0.0003 0.0013 0.0031 0.0045 0.0031 0.0016 0.0001 0.0003 0 0.0559 0.0001
Lipid Particles 0.0104 0 0.0008 0.0001 0.0007 0.0038 0.0012 0.0001 0.0021 0.0017 0.0199 0.002 0.0014 0.0001 0.0009 0 0.0059 0
Mitochondria 0.0027 0.0001 0.0005 0.0002 0.0001 0.0016 0.0008 0.0004 0.0086 0.0017 0.0026 0.0008 0.0004 0.0001 0.0001 0.0001 0.0812 0.0001
None 0.0958 0.0951 0.0544 0.0874 0.1399 0.0765 0.0646 0.0416 0.0417 0.1349 0.0256 0.0191 0.1313 0.1299 0.1257 0.0547 0.018 0.0122
Nuclear Periphery 0.0186 0.0006 0.0021 0.0006 0.0036 0.0018 0.0067 0.001 0.0035 0.0016 0.0039 0.0028 0.019 0.0003 0.0012 0.0004 0.0004 0.001
Nucleolus 0.0003 0 0.0001 0 0.0001 0.0001 0.0003 0 0.0008 0.0001 0.0003 0.0001 0.0001 0 0.0002 0 0.0001 0
Nucleus 0.0076 0.0016 0.0073 0.0023 0.0049 0.0066 0.0058 0.0029 0.0078 0.0033 0.007 0.0058 0.0129 0.0009 0.0021 0.0032 0.0033 0.0067
Peroxisomes 0.0179 0 0.0021 0.0002 0.0001 0.0005 0.0027 0.0001 0.0089 0.0165 0.0184 0.0028 0.0014 0 0.0003 0 0.0267 0
Punctate Nuclear 0.0062 0.0002 0.0027 0.0002 0.0024 0.0011 0.0018 0.0004 0.0013 0.0044 0.0061 0.0043 0.0029 0.0001 0.0103 0.0002 0.0006 0.0018
Vacuole 0.0014 0.0006 0.0018 0.0008 0.0005 0.0008 0.0026 0.0022 0.0019 0.0008 0.0017 0.0011 0.0006 0.0002 0.0005 0.0004 0.0016 0.0002
Vacuole Periphery 0.0011 0.0001 0.0003 0.0001 0.0001 0.0003 0.0008 0.0007 0.001 0.0002 0.0004 0.0003 0.0004 0 0.0001 0.0001 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.6822 37.623 27.9179 42.1677 37.9599 64.0096 33.0787 35.1633 43.411 38.4273
Translational Efficiency 1.8132 1.1707 1.8423 1.3965 1.5821 1.0873 1.7443 1.7679 1.9093 1.7819

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
411 1296 110 1548 297 2375 2720 1202 708 3671 2830 2750

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 694.14 750.56 1247.82 956.69 795.60 828.16 993.31 1001.95 736.70 800.76 1003.20 976.47
Standard Deviation 84.41 120.78 177.14 170.09 97.28 110.59 211.55 187.17 103.02 120.16 215.99 179.17
Intensity Change Log 2 — 0.112741 0.846111 0.462825 — 0.057866 0.320201 0.332695 — 0.083695 0.589166 0.394792

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000272 0.000272 0.001331 0.001187 0.000107 0.000284 0.000114 0.000760 0.000203 0.000279 0.000161 0.001001
Bud Neck 0.024684 0.016411 0.003202 0.005471 0.005260 0.017756 0.003624 0.010216 0.016536 0.017281 0.003608 0.007545
Bud Site 0.010003 0.016860 0.014860 0.040456 0.002288 0.013044 0.003762 0.052080 0.006766 0.014391 0.004193 0.045537
Cell Periphery 0.000369 0.000124 0.000293 0.000128 0.000151 0.000092 0.000073 0.000108 0.000278 0.000103 0.000081 0.000119
Cytoplasm 0.437355* 0.578681* 0.415636* 0.633036* 0.562013* 0.642165* 0.690861* 0.629643* 0.489648* 0.619753* 0.680163* 0.631553*
Cytoplasmic Foci 0.304671* 0.153338 0.013531 0.014450 0.178910 0.099566 0.004449 0.017504 0.251916* 0.118550 0.004802 0.015785
Eisosomes 0.000315 0.000061 0.000066 0.000028 0.000093 0.000056 0.000008 0.000021 0.000222 0.000058 0.000010 0.000025
Endoplasmic Reticulum 0.001721 0.001329 0.007393 0.001429 0.001038 0.000438 0.000477 0.000889 0.001435 0.000753 0.000746 0.001193
Endosome 0.013000 0.014966 0.005779 0.002829 0.008477 0.006785 0.000420 0.006034 0.011103 0.009674 0.000628 0.004230
Golgi 0.006670 0.003155 0.000343 0.000594 0.000265 0.001710 0.000071 0.002753 0.003983 0.002220 0.000081 0.001538
Lipid Particles 0.010145 0.003087 0.003336 0.000806 0.004378 0.001521 0.000269 0.000725 0.007726 0.002074 0.000388 0.000770
Mitochondria 0.014532 0.002321 0.000635 0.001476 0.000311 0.003204 0.000404 0.008009 0.008567 0.002893 0.000413 0.004331
Mitotic Spindle 0.000728 0.004198 0.020634 0.025120 0.001400 0.011011 0.018478 0.041530 0.001009 0.008606 0.018562 0.032293
None 0.002947 0.007023 0.003341 0.005368 0.002428 0.004779 0.012156 0.005693 0.002729 0.005571 0.011813 0.005510
Nuclear Periphery 0.000300 0.000422 0.001709 0.000711 0.000504 0.000356 0.000611 0.000647 0.000385 0.000379 0.000654 0.000683
Nuclear Periphery Foci 0.000417 0.001025 0.007764 0.001861 0.000901 0.000231 0.000497 0.001467 0.000620 0.000511 0.000780 0.001689
Nucleolus 0.001336 0.002296 0.000249 0.000140 0.000615 0.000960 0.000191 0.000346 0.001034 0.001432 0.000194 0.000230
Nucleus 0.114668 0.111489 0.333309* 0.157272* 0.168077* 0.132245* 0.238241* 0.118383 0.137073* 0.124917 0.241937* 0.140274*
Peroxisomes 0.013155 0.007170 0.000454 0.001470 0.001201 0.004845 0.000137 0.001497 0.008140 0.005666 0.000149 0.001482
Vacuole 0.040904 0.074423 0.165857 0.105731 0.061248 0.058169 0.025097 0.095859 0.049438 0.063907 0.030568 0.101416
Vacuole Periphery 0.001808 0.001348 0.000279 0.000438 0.000335 0.000783 0.000060 0.005835 0.001190 0.000982 0.000068 0.002797

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.06 -5.00 -2.32 -2.30 2.97 -2.68 -2.65 -3.01 -0.93 -2.26 -1.59 -0.94 -3.32 -2.38 -3.10
Bud Neck 2.41 6.13 6.51 8.37 1.46 -6.48 0.66 -0.09 8.38 -1.20 -0.30 6.01 5.87 12.79 -0.27
Bud Site -2.00 -0.18 -2.24 -0.47 -1.89 -8.91 -2.88 -6.68 -2.24 -6.07 -4.03 1.58 -5.08 -2.18 -8.47
Cell Periphery 6.81 0.84 7.37 1.35 6.85 4.30 4.99 5.46 1.56 1.23 8.02 8.46 8.46 1.01 -0.16
Cytoplasm -8.34 3.55 -5.62 4.44 -7.20 -5.26 -1.87 1.98 11.86 6.60 -11.25 -7.68 -3.13 13.14 7.24
Cytoplasmic Foci 12.02 24.94 25.14 28.03 -0.08 7.65 17.42 16.65 28.86 -7.80 15.41 29.83 28.96 39.98 -11.82
Eisosomes 8.39 7.87 9.38 9.43 4.47 5.13 12.19 9.97 6.97 -12.83 9.02 11.47 10.60 9.57 -15.73
Endoplasmic Reticulum 0.78 -4.57 0.33 -1.31 4.95 2.88 1.36 0.40 -7.58 -3.13 2.49 1.43 0.49 -7.06 -3.14
Endosome -1.18 4.89 8.66 11.90 5.31 1.64 7.79 5.83 8.25 -4.39 1.46 11.91 10.38 15.17 -5.91
Golgi 2.60 5.11 5.17 4.35 0.95 -4.81 4.21 -1.51 2.42 -2.21 2.32 5.34 4.78 5.34 -2.65
Lipid Particles 5.24 4.11 6.84 9.57 3.61 5.45 7.32 6.65 5.08 -6.05 6.95 8.68 8.33 10.19 -5.70
Mitochondria 3.53 4.03 4.00 4.00 -0.45 -5.88 -5.42 -2.22 1.02 -1.96 2.79 3.97 3.51 2.96 -2.06
Mitotic Spindle -3.30 -1.65 -6.95 -4.87 -1.06 -4.56 -8.72 -6.81 -3.60 -0.42 -6.13 -10.77 -9.76 -4.97 0.81
None -3.31 0.23 -3.57 1.39 -2.79 -3.18 -14.94 -7.60 -3.23 6.91 -4.22 -15.33 -7.25 -1.12 10.84
Nuclear Periphery -2.77 -10.71 -17.65 -14.18 5.94 2.55 -10.01 -9.39 -15.78 -0.72 0.05 -18.38 -18.86 -22.36 0.32
Nuclear Periphery Foci -5.94 -5.15 -11.59 -4.52 4.26 5.28 1.67 -2.50 -8.00 -4.61 0.96 -3.36 -9.36 -11.25 -5.18
Nucleolus -1.93 2.49 2.66 7.27 2.53 -2.58 5.58 4.27 5.31 -1.95 -1.49 3.14 3.14 9.19 0.04
Nucleus 0.23 -7.97 -9.10 -13.95 4.99 3.38 -12.15 -0.20 -6.25 19.73 1.65 -21.43 -6.88 -14.08 21.13
Peroxisomes 2.86 7.18 7.04 5.88 -0.71 -5.65 6.14 3.82 7.09 -3.16 2.07 7.59 7.18 9.05 -2.78
Vacuole -7.93 -9.53 -23.80 -18.25 2.16 0.71 0.03 -16.64 -21.37 -20.57 -4.41 -5.05 -28.56 -29.44 -26.99
Vacuole Periphery 1.89 6.17 6.44 10.91 1.77 -3.63 7.82 -1.54 -0.90 -1.94 1.38 7.58 1.41 0.78 -2.14
ORF
Human Ortholog
Description Protein of unknown function; predicted to encode a pyridoxal 5'-phosphate synthase based on sequence similarity but purified protein does not possess this activity, nor does it bind flavin mononucleotide (FMN); transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (40%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YPR172W

YPR172W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypr172w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available