Standard name | |
---|---|
Human Ortholog | |
Description | Dolichol phosphate mannose (Dol-P-Man) synthase of ER membrane; catalyzes formation of Dol-P-Man from Dol-P and GDP-Man; required for biosynthesis of glycosyl phosphatidylinositol (GPI) membrane anchor, as well as O-mannosylation and protein N- and O-linked glycosylation; human homolog DPM1 can complement yeast mutant strains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 8 | 20 | 19 | 69 | 23 | 25 | 25 | 22 | 34 | 20 | 41 | 7 | 25 | 6 | 16 | 30 | 14 | 2 | 1 | 2 | 3 | 4 | 9 |
Bud | 3 | 1 | 4 | 4 | 11 | 4 | 3 | 16 | 15 | 11 | 3 | 8 | 1 | 0 | 1 | 4 | 7 | 2 | 2 | 0 | 0 | 0 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 9 | 22 | 19 | 42 | 17 | 17 | 16 | 18 | 24 | 10 | 31 | 7 | 52 | 60 | 24 | 26 | 7 | 1 | 1 | 5 | 2 | 5 | 10 |
Cytoplasm | 8 | 14 | 9 | 19 | 30 | 26 | 47 | 56 | 59 | 61 | 35 | 65 | 5 | 13 | 12 | 25 | 26 | 16 | 2 | 3 | 1 | 1 | 5 | 13 |
Endoplasmic Reticulum | 36 | 122 | 137 | 186 | 323 | 169 | 273 | 168 | 224 | 255 | 199 | 280 | 101 | 282 | 243 | 5 | 4 | 2 | 69 | 135 | 151 | 143 | 217 | 266 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 2 | 7 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Golgi | 3 | 0 | 2 | 10 | 17 | 0 | 2 | 2 | 0 | 5 | 4 | 3 | 6 | 8 | 1 | 48 | 49 | 40 | 0 | 0 | 0 | 2 | 0 | 0 |
Mitochondria | 14 | 0 | 4 | 9 | 27 | 24 | 50 | 52 | 57 | 60 | 80 | 101 | 0 | 0 | 0 | 8 | 11 | 6 | 2 | 0 | 4 | 3 | 1 | 2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 5 | 17 | 7 | 8 | 9 | 13 | 17 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 1 | 1 | 2 | 24 | 4 | 14 | 70 | 39 | 91 | 23 | 22 | 3 | 1 | 2 | 8 | 6 | 5 | 1 | 2 | 0 | 2 | 4 | 6 |
Unique Cell Count | 57 | 133 | 156 | 216 | 412 | 216 | 359 | 347 | 349 | 460 | 299 | 444 | 113 | 318 | 274 | 126 | 137 | 81 | 85 | 148 | 168 | 161 | 245 | 321 |
Labelled Cell Count | 77 | 155 | 199 | 269 | 546 | 272 | 449 | 420 | 445 | 560 | 390 | 570 | 130 | 381 | 325 | 141 | 162 | 93 | 85 | 148 | 168 | 161 | 245 | 321 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 17.5 | 24.6 | 16.5 | 17.2 | 14.7 | 12.3 | 11.4 | 11.8 | 11.6 | 11.3 | 11.4 | 11.3 | 21.6 | 21.9 | 22.1 | 24.1 | 24.3 | 26.1 | 23.7 | 23.9 | 25.5 |
Std Deviation (1e-4) | 4.9 | 4.0 | 2.7 | 3.3 | 2.5 | 2.0 | 2.2 | 2.3 | 2.1 | 2.5 | 2.5 | 2.3 | 3.9 | 3.7 | 3.8 | 6.6 | 5.8 | 7.2 | 4.5 | 4.4 | 5.4 |
Intensity Change (Log2) | – | – | – | 0.05 | -0.17 | -0.43 | -0.54 | -0.49 | -0.51 | -0.54 | -0.54 | -0.56 | 0.39 | 0.4 | 0.42 | 0.55 | 0.55 | 0.66 | 0.52 | 0.53 | 0.62 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.3 | 1.1 | -0.6 | -2.2 | -2.0 | -2.5 | -2.1 | -2.2 | -1.3 | -1.8 | -1.7 | -4.4 | 0 | 2.1 | 0.9 |
Bud | 0 | 0 | 0 | 0 | 1.1 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.6 | -1.3 | -1.9 | -3.7 | -3.7 | -3.4 | -3.6 | -4.3 | -2.7 | -2.1 | 0.6 | 2.0 | 1.1 | 1.1 | -1.2 |
Cytoplasm | 1.1 | 0.6 | 2.0 | 2.5 | 3.2 | 3.4 | 2.5 | 2.0 | 2.9 | -0.5 | -0.8 | -0.6 | 3.6 | 3.5 | 3.3 |
Endoplasmic Reticulum | -0.5 | -2.6 | -2.4 | -3.0 | -8.4 | -5.4 | -7.3 | -4.9 | -5.8 | 0.4 | 0.3 | 0.3 | -14.0 | -14.5 | -12.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1.8 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.0 | 7.8 | 9.2 |
Mitochondria | 0.8 | 1.9 | 3.1 | 3.9 | 4.1 | 4.4 | 3.7 | 6.3 | 5.7 | 0 | 0 | 0 | 1.6 | 2.1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.7 | 0 | 0 | 5.8 | 4.0 | 5.8 | 3.2 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 22.6109 | 23.6754 | 23.7385 | 23.7599 | 20.639 | 22.3024 | 20.9281 | 24.3039 | 21.7987 | 21.815 | 21.2101 | 21.7831 | 24.5137 | 23.9381 | 24.3944 | 22.5243 | 22.5099 | 22.8826 |
Actin | 0.0211 | 0.0005 | 0.0018 | 0.0003 | 0.0002 | 0.0018 | 0.0058 | 0.0012 | 0.0093 | 0.0193 | 0.0035 | 0.0012 | 0.0029 | 0.0042 | 0.0035 | 0.0001 | 0.0002 | 0.0014 |
Bud | 0.0004 | 0 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0 | 0 |
Bud Neck | 0.0004 | 0.0003 | 0.0007 | 0.0004 | 0.0002 | 0.0025 | 0.0006 | 0.0005 | 0.0008 | 0.0005 | 0.0008 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0005 |
Bud Periphery | 0.0012 | 0.0001 | 0.0018 | 0.0006 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0041 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0012 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0004 | 0.0013 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0005 | 0 | 0 | 0 |
Cell Periphery | 0.0165 | 0.0184 | 0.0296 | 0.0207 | 0.0176 | 0.0108 | 0.0125 | 0.0255 | 0.0129 | 0.0114 | 0.01 | 0.0073 | 0.0211 | 0.0238 | 0.03 | 0.0136 | 0.0069 | 0.0081 |
Cytoplasm | 0.0128 | 0.006 | 0.0084 | 0.0023 | 0.0032 | 0.0033 | 0.0171 | 0.0056 | 0.0048 | 0.0018 | 0.008 | 0.003 | 0.0074 | 0.0058 | 0.0051 | 0.0011 | 0.0023 | 0.0022 |
Cytoplasmic Foci | 0.0084 | 0.002 | 0.0012 | 0.0008 | 0.0008 | 0.0008 | 0.0127 | 0.0033 | 0.0051 | 0.0037 | 0.0022 | 0.002 | 0.0022 | 0.0014 | 0.0011 | 0.0002 | 0.0002 | 0.0009 |
Eisosomes | 0.0012 | 0.0003 | 0.0022 | 0.0006 | 0.0008 | 0.0006 | 0.0007 | 0.0006 | 0.0005 | 0.0012 | 0.0011 | 0.0003 | 0.0008 | 0.0003 | 0.0017 | 0.0002 | 0.0004 | 0.0007 |
Endoplasmic Reticulum | 0.7233 | 0.7812 | 0.8141 | 0.8083 | 0.8566 | 0.8819 | 0.7902 | 0.7582 | 0.8222 | 0.8421 | 0.8175 | 0.861 | 0.7366 | 0.8102 | 0.7806 | 0.9551 | 0.9641 | 0.8857 |
Endosome | 0.0141 | 0.0053 | 0.0058 | 0.0038 | 0.0037 | 0.0046 | 0.0153 | 0.0066 | 0.0148 | 0.0053 | 0.01 | 0.0094 | 0.01 | 0.0036 | 0.0034 | 0.0013 | 0.0017 | 0.0069 |
Golgi | 0.0174 | 0.0177 | 0.0121 | 0.0181 | 0.0142 | 0.0127 | 0.0176 | 0.0236 | 0.0291 | 0.0184 | 0.0211 | 0.0196 | 0.0407 | 0.0208 | 0.0199 | 0.0024 | 0.009 | 0.0323 |
Lipid Particles | 0.1413 | 0.1546 | 0.0976 | 0.1243 | 0.0898 | 0.0681 | 0.0692 | 0.1635 | 0.076 | 0.0836 | 0.1084 | 0.0818 | 0.1277 | 0.1175 | 0.1282 | 0.0164 | 0.007 | 0.0516 |
Mitochondria | 0.0043 | 0.0002 | 0.0015 | 0.0019 | 0.0002 | 0.0003 | 0.0011 | 0.0003 | 0.0015 | 0.004 | 0.0021 | 0.001 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0008 |
None | 0.0017 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0151 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0212 | 0.0047 | 0.0083 | 0.0035 | 0.0062 | 0.0068 | 0.0269 | 0.0035 | 0.0069 | 0.0035 | 0.0074 | 0.0064 | 0.0326 | 0.0058 | 0.0085 | 0.0055 | 0.0049 | 0.0055 |
Nucleolus | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.002 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0012 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.0008 | 0 | 0 | 0 | 0 | 0 | 0.0011 | 0.0001 | 0.004 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0026 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Vacuole | 0.0093 | 0.0074 | 0.0112 | 0.0129 | 0.0052 | 0.0044 | 0.0067 | 0.0063 | 0.0072 | 0.0021 | 0.0041 | 0.0034 | 0.0127 | 0.0054 | 0.01 | 0.0032 | 0.0023 | 0.0023 |
Vacuole Periphery | 0.0016 | 0.0004 | 0.002 | 0.001 | 0.0007 | 0.0007 | 0.0019 | 0.0004 | 0.0013 | 0.0006 | 0.002 | 0.0012 | 0.0027 | 0.0003 | 0.0014 | 0.0003 | 0.0004 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 220.9554 | 175.4376 | 147.4792 | 143.7648 | 185.0543 | 207.1167 | 179.5555 | 147.7697 | 153.1766 | 172.7041 |
Translational Efficiency | 1.7585 | 2.0699 | 1.8503 | 2.3148 | 2.5112 | 2.4806 | 1.8945 | 2.5904 | 2.1062 | 2.3136 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2721 | 1551 | 170 | 1241 | 2433 | 1541 | 333 | 1537 | 5154 | 3092 | 503 | 2778 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1590.31 | 1940.50 | 2744.62 | 2598.17 | 1906.97 | 1786.60 | 2665.89 | 2693.49 | 1739.79 | 1863.80 | 2692.50 | 2650.91 |
Standard Deviation | 259.81 | 381.75 | 468.53 | 488.84 | 304.80 | 329.94 | 367.09 | 507.48 | 323.24 | 365.07 | 405.94 | 501.48 |
Intensity Change Log 2 | — | 0.287120 | 0.787298 | 0.708188 | — | -0.094065 | 0.483335 | 0.498195 | — | 0.091820 | 0.629531 | 0.597487 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000108 | 0.000950 | 0.000661 | 0.003540 | 0.000111 | 0.001553 | 0.001162 | 0.002075 | 0.000109 | 0.001251 | 0.000993 | 0.002729 |
Bud Neck | 0.000048 | 0.000489 | 0.011053 | 0.002124 | 0.000126 | 0.000685 | 0.002914 | 0.000999 | 0.000085 | 0.000587 | 0.005664 | 0.001501 |
Bud Site | 0.000460 | 0.002066 | 0.004237 | 0.006136 | 0.000448 | 0.003464 | 0.001824 | 0.003644 | 0.000454 | 0.002763 | 0.002640 | 0.004757 |
Cell Periphery | 0.008696 | 0.013299 | 0.190356* | 0.137921* | 0.010080 | 0.013721 | 0.080303 | 0.118568* | 0.009349 | 0.013509 | 0.117497* | 0.127213* |
Cytoplasm | 0.001181 | 0.001326 | 0.000135 | 0.001599 | 0.000456 | 0.001248 | 0.000049 | 0.000817 | 0.000838 | 0.001287 | 0.000078 | 0.001166 |
Cytoplasmic Foci | 0.000954 | 0.002452 | 0.000246 | 0.001591 | 0.000330 | 0.001602 | 0.000099 | 0.001753 | 0.000660 | 0.002029 | 0.000149 | 0.001681 |
Eisosomes | 0.000227 | 0.000656 | 0.002836 | 0.003160 | 0.000363 | 0.000505 | 0.007084 | 0.003081 | 0.000291 | 0.000581 | 0.005648 | 0.003116 |
Endoplasmic Reticulum | 0.973655* | 0.923832* | 0.591679* | 0.578026* | 0.965721* | 0.942873* | 0.786668* | 0.629382* | 0.969910* | 0.933322* | 0.720767* | 0.606440* |
Endosome | 0.002687 | 0.010205 | 0.014141 | 0.010369 | 0.003741 | 0.006854 | 0.007976 | 0.010687 | 0.003185 | 0.008535 | 0.010060 | 0.010545 |
Golgi | 0.001841 | 0.009218 | 0.022466 | 0.025862 | 0.003616 | 0.005249 | 0.019697 | 0.035294 | 0.002679 | 0.007240 | 0.020633 | 0.031081 |
Lipid Particles | 0.004952 | 0.027053 | 0.056694 | 0.174596* | 0.011138 | 0.014503 | 0.056002 | 0.147610* | 0.007872 | 0.020798 | 0.056236 | 0.159666* |
Mitochondria | 0.000390 | 0.000226 | 0.000122 | 0.000934 | 0.000172 | 0.000742 | 0.000132 | 0.001758 | 0.000287 | 0.000483 | 0.000129 | 0.001390 |
Mitotic Spindle | 0.000074 | 0.000106 | 0.000470 | 0.001678 | 0.000074 | 0.000537 | 0.000272 | 0.001425 | 0.000074 | 0.000321 | 0.000339 | 0.001538 |
None | 0.001128 | 0.000387 | 0.000023 | 0.000290 | 0.000633 | 0.000258 | 0.000065 | 0.000290 | 0.000894 | 0.000323 | 0.000051 | 0.000290 |
Nuclear Periphery | 0.000421 | 0.000644 | 0.007617 | 0.011027 | 0.000686 | 0.000468 | 0.006675 | 0.009947 | 0.000546 | 0.000556 | 0.006993 | 0.010430 |
Nuclear Periphery Foci | 0.000185 | 0.001469 | 0.007149 | 0.003330 | 0.000296 | 0.000712 | 0.001363 | 0.001780 | 0.000238 | 0.001092 | 0.003319 | 0.002472 |
Nucleolus | 0.000004 | 0.000005 | 0.000015 | 0.000030 | 0.000003 | 0.000121 | 0.000011 | 0.000033 | 0.000003 | 0.000063 | 0.000012 | 0.000031 |
Nucleus | 0.000043 | 0.000056 | 0.000179 | 0.000233 | 0.000037 | 0.000132 | 0.000074 | 0.000200 | 0.000040 | 0.000094 | 0.000110 | 0.000214 |
Peroxisomes | 0.002234 | 0.000877 | 0.000027 | 0.001257 | 0.000779 | 0.000995 | 0.000060 | 0.002743 | 0.001547 | 0.000936 | 0.000049 | 0.002079 |
Vacuole | 0.000488 | 0.003637 | 0.084881 | 0.031190 | 0.000924 | 0.002624 | 0.025440 | 0.022601 | 0.000694 | 0.003132 | 0.045529 | 0.026438 |
Vacuole Periphery | 0.000225 | 0.001046 | 0.005013 | 0.005106 | 0.000266 | 0.001152 | 0.002129 | 0.005314 | 0.000244 | 0.001099 | 0.003104 | 0.005221 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.95 | -3.95 | -4.13 | -2.97 | -3.42 | -2.88 | -2.91 | -3.60 | -0.76 | -1.45 | -3.97 | -3.65 | -5.48 | -2.71 | -3.29 |
Bud Neck | -6.15 | -3.43 | -9.20 | -6.96 | 2.79 | -3.60 | -5.29 | -9.51 | -1.82 | 3.56 | -5.89 | -4.82 | -12.51 | -6.58 | 3.59 |
Bud Site | -3.48 | -3.37 | -4.99 | -3.39 | -1.20 | -3.84 | -2.41 | -4.92 | -0.33 | -2.26 | -5.09 | -4.08 | -6.92 | -2.76 | -2.68 |
Cell Periphery | -3.78 | -10.32 | -23.53 | -22.36 | 2.83 | -2.72 | -9.64 | -23.99 | -22.73 | -4.49 | -4.61 | -13.55 | -33.62 | -31.87* | -1.13 |
Cytoplasm | -0.42 | 4.14 | -1.08 | -0.57 | -3.61 | -2.56 | 3.99 | -2.00 | 1.20 | -4.76 | -1.67 | 5.40 | -1.56 | 0.24 | -5.35 |
Cytoplasmic Foci | -2.32 | 3.51 | -1.22 | 1.07 | -2.68 | -3.55 | 3.58 | -4.21 | -0.29 | -4.95 | -3.69 | 4.74 | -3.34 | 0.76 | -5.27 |
Eisosomes | -4.55 | -4.46 | -8.57 | -7.19 | -0.49 | -1.37 | -4.06 | -10.58 | -9.69 | 2.39 | -4.14 | -4.80 | -13.54 | -11.87 | 2.23 |
Endoplasmic Reticulum | 12.46 | 14.80 | 43.39* | 35.26* | 0.47 | 6.11 | 12.95 | 42.43* | 37.02* | 9.96 | 13.36 | 18.77 | 60.61 | 50.75* | 7.85 |
Endosome | -10.73 | -5.05 | -13.34 | -0.22 | 1.66 | -6.09 | -5.16 | -13.54 | -5.63 | -2.86 | -12.31 | -7.26 | -19.30 | -3.61 | -0.41 |
Golgi | -9.74 | -6.52 | -19.83 | -11.72 | -1.00 | -3.09 | -6.88 | -19.29 | -17.61 | -5.48 | -9.85 | -9.56 | -26.77 | -20.77 | -4.85 |
Lipid Particles | -10.33 | -5.19 | -25.43 | -21.15 | -9.67 | -2.05 | -5.96 | -24.43 | -23.57 | -9.78 | -9.59 | -8.07 | -35.36 | -31.31 | -13.92 |
Mitochondria | 2.30 | 4.31 | -1.84 | -2.38 | -2.74 | -2.12 | 1.23 | -2.75 | -1.61 | -2.81 | -1.44 | 4.32 | -3.19 | -2.46 | -3.64 |
Mitotic Spindle | -1.54 | -3.24 | -4.60 | -4.52 | -3.49 | -1.48 | -6.05 | -2.92 | -1.58 | -2.51 | -1.58 | -5.58 | -5.05 | -3.82 | -4.19 |
None | 3.04 | 5.55 | 4.12 | 0.66 | -6.92 | 2.60 | 4.83 | 2.68 | -0.34 | -4.71 | 3.95 | 7.08 | 4.89 | 0.37 | -7.42 |
Nuclear Periphery | -4.27 | -10.46 | -18.73 | -18.29 | -3.78 | 4.20 | -10.08 | -20.49 | -21.02 | -4.39 | -0.30 | -14.13 | -27.79 | -27.73 | -5.91 |
Nuclear Periphery Foci | -5.28 | -5.82 | -7.04 | -3.67 | 3.01 | -4.48 | -6.75 | -16.83 | -8.62 | -2.38 | -6.53 | -7.06 | -10.82 | -5.67 | 1.78 |
Nucleolus | -0.89 | -5.43 | -11.22 | -9.16 | -4.46 | -1.34 | -5.96 | -9.65 | 1.03 | -6.53 | -1.35 | -7.84 | -14.00 | 0.77 | -8.25 |
Nucleus | -1.69 | -3.85 | -9.02 | -7.92 | -1.36 | -2.09 | -4.30 | -9.20 | -1.23 | -6.52 | -2.34 | -5.17 | -12.85 | -4.33 | -5.57 |
Peroxisomes | 3.69 | 7.35 | 2.36 | -1.05 | -4.27 | -0.82 | 4.82 | -3.64 | -3.06 | -5.17 | 2.60 | 8.61 | -1.47 | -3.24 | -6.44 |
Vacuole | -5.34 | -10.66 | -18.90 | -15.88 | 6.63 | -4.09 | -9.39 | -16.60 | -14.62 | 0.87 | -6.75 | -13.11 | -25.08 | -21.39 | 5.29 |
Vacuole Periphery | -2.92 | -6.46 | -11.11 | -7.78 | -0.10 | -3.39 | -6.96 | -9.80 | -7.25 | -5.57 | -4.45 | -9.15 | -14.37 | -10.44 | -4.61 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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