Standard name
Human Ortholog
Description Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.05 0.07 0.1 0 0.07 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.97 0.97 0.97 0.91 0.85 0.74 0.74 0.73 0.65 0.67 1.0 0.97 0.96 0.96 0.96 0.96 0.89 0.94 0.83 0.83 0.91 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0.06 0.12 0.05 0.18 0.18 0.27 0.4 0.32 0.43 0.47 0 0 0 0 0 0 0.05 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0.12 0.08 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 1 0 1
Bud 0 0 4 1 0 2 9 16 5 8 3 12 0 3 11 0 0 0 0 0 0 1 3 3
Bud Neck 0 0 3 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 7 9 15 21 16 32 9 20 0 0 10 9 7 8 1 0 0 0 0 0
Cytoplasm 202 179 156 117 225 279 304 333 173 234 149 193 156 230 237 154 126 144 194 179 133 74 182 207
Endoplasmic Reticulum 1 0 1 0 1 4 2 6 1 5 3 5 0 1 1 10 6 10 0 0 2 0 0 2
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 3 1 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 6 18 9 14 12 55 66 121 94 102 98 135 1 3 6 4 0 0 11 6 11 1 0 5
Nucleus 0 0 1 0 0 2 2 2 2 2 3 1 0 0 1 0 0 0 0 0 1 0 1 0
Nuclear Periphery 1 2 1 0 0 0 2 2 4 0 3 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 3 0 0 0 1 0 2 2 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 3 1 0 1 4 2 23 53 18 14 13 13 0 3 2 4 2 5 3 0 1 4 5 3
Unique Cell Count 208 187 161 121 233 305 357 451 234 319 230 287 156 238 247 161 131 150 217 192 161 90 201 237
Labelled Cell Count 214 200 175 133 250 354 424 559 314 398 282 384 157 243 271 183 143 171 217 192 161 90 201 237


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 3.6 3.8 3.3 4.9 5.1 5.5 5.7 5.5 5.8 5.6 5.3 5.0 6.6 6.6 6.7 7.2 7.3 4.7 4.5 5.4
Std Deviation (1e-4) 2.3 0.8 1.5 1.0 2.1 1.7 2.1 1.9 2.1 2.2 2.1 2.3 1.4 2.5 2.7 2.1 2.5 3.5 1.2 1.5 1.5
Intensity Change (Log2) – – – -0.18 0.39 0.44 0.54 0.6 0.55 0.61 0.56 0.49 0.4 0.81 0.82 0.82 0.94 0.96 0.31 0.24 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.3906 29.9745 20.8799 40.06 2.8787 25.089 1.8039 2.7298 2.7234 1.6608 2.3483 2.2225 2.6896 2.855 2.1273 1.8236 2.8721 2.3111
Actin 0.0117 0.0047 0.0115 0.0021 0.0022 0.01 0.0308 0.0011 0.0029 0.0003 0.0243 0.0077 0.0311 0.0657 0.0473 0.0012 0.0007 0.0022
Bud 0.0055 0.0172 0.0297 0.0112 0.0022 0.0031 0.0015 0.0036 0.0077 0.0006 0.0032 0.0024 0.0013 0.0008 0.0012 0.0001 0.0003 0.003
Bud Neck 0.0017 0.0008 0.0044 0.0013 0.0005 0.0024 0.0064 0.0002 0.0009 0.0001 0.0019 0.0029 0.0012 0.0008 0.0006 0.0002 0.0001 0.0006
Bud Periphery 0.0134 0.0059 0.02 0.0083 0.002 0.0027 0.0019 0.0021 0.0046 0.0006 0.0038 0.0025 0.0031 0.0008 0.0011 0 0.0011 0.0049
Bud Site 0.0061 0.0229 0.02 0.0016 0.0013 0.0006 0.0055 0.0132 0.0049 0.0002 0.0183 0.0018 0.0069 0.0322 0.0031 0.0002 0.0001 0.0002
Cell Periphery 0.0138 0.0089 0.0122 0.0171 0.0011 0.003 0.0002 0.0002 0.0005 0 0.0003 0.0002 0.0015 0.0012 0.0002 0.0001 0 0.0001
Cytoplasm 0.6109 0.7633 0.6734 0.6092 0.7406 0.7833 0.4176 0.6577 0.5896 0.4388 0.5209 0.671 0.4323 0.6502 0.5772 0.6484 0.3906 0.59
Cytoplasmic Foci 0.0331 0.0205 0.0131 0.0225 0.0292 0.0265 0.031 0.0077 0.0097 0.0076 0.0147 0.0146 0.0329 0.0021 0.0052 0.0073 0.0128 0.0268
Eisosomes 0.0004 0.0001 0.0002 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0003 0.0021 0.0002 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.0301 0.0027 0.0051 0.0173 0.0048 0.0067 0.0056 0.0017 0.0062 0.0013 0.0022 0.0041 0.0339 0.0013 0.0012 0.0024 0.0014 0.0011
Endosome 0.0164 0.007 0.0059 0.0053 0.0156 0.0115 0.0207 0.0012 0.0051 0.0013 0.0195 0.0036 0.031 0.0003 0.002 0.003 0.002 0.0018
Golgi 0.003 0.0011 0.0066 0.0005 0.0019 0.0032 0.0202 0.0002 0.0005 0.0001 0.0044 0.0016 0.0155 0.0004 0.0044 0.0003 0.0009 0.0005
Lipid Particles 0.0101 0.0008 0.0079 0.0013 0.0103 0.0063 0.019 0.0001 0.0005 0.0002 0.0036 0.0011 0.0382 0.0002 0.003 0.0004 0.0087 0.0003
Mitochondria 0.0123 0.0007 0.0131 0.001 0.0196 0.0089 0.0197 0.0002 0.0085 0.0003 0.0022 0.0021 0.034 0.0001 0.0059 0.0002 0.0046 0.0004
None 0.0943 0.0844 0.0951 0.0581 0.0882 0.0537 0.3801 0.3044 0.3424 0.5436 0.3443 0.2693 0.2905 0.2412 0.338 0.3305 0.5521 0.3639
Nuclear Periphery 0.0284 0.0022 0.0045 0.016 0.0178 0.0091 0.0036 0.0009 0.0031 0.0007 0.0021 0.0026 0.0073 0.0002 0.0004 0.0012 0.0008 0.001
Nucleolus 0.002 0.0008 0.0061 0.0008 0.0137 0.0091 0.0006 0.0001 0.0003 0.0001 0.0041 0.0006 0.0008 0 0.0001 0.0001 0.0002 0.0001
Nucleus 0.009 0.0043 0.0078 0.0087 0.0113 0.0075 0.0138 0.0017 0.0032 0.0015 0.0059 0.0031 0.0021 0.0004 0.0007 0.0015 0.0006 0.001
Peroxisomes 0.0055 0.0013 0.0063 0.0004 0.002 0.0017 0.017 0.0003 0.0004 0.0002 0.0061 0.0042 0.0276 0.0009 0.0063 0.0002 0.0208 0.0006
Punctate Nuclear 0.0054 0.0036 0.0026 0.0061 0.0096 0.0076 0.0022 0.0023 0.0023 0.0019 0.003 0.0027 0.0022 0.0002 0.0012 0.0022 0.0011 0.0007
Vacuole 0.0735 0.0437 0.0502 0.1817 0.0116 0.0335 0.0017 0.0008 0.0061 0.0005 0.0139 0.0013 0.0032 0.0009 0.0005 0.0003 0.0005 0.0007
Vacuole Periphery 0.0134 0.0032 0.0044 0.0296 0.0144 0.0096 0.0007 0.0001 0.0007 0.0001 0.0012 0.0004 0.0013 0 0.0001 0.0001 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.1526 5.5619 4.2859 9.0833 8.0668 13.8716 8.5976 9.2699 15.5452 12.9258
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
116 1297 285 1038 1358 1549 1879 553 1474 2846 2164 1591

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1264.07 727.91 2338.18 890.32 644.76 738.73 913.38 852.47 693.50 733.80 1101.03 877.16
Standard Deviation 400.97 94.05 509.59 141.35 79.84 106.58 120.22 164.74 215.25 101.21 528.11 150.97
Intensity Change Log 2 — -0.796244 0.887310 -0.505680 — 0.196285 0.502453 0.402887 — -0.380174 0.768443 -0.131290

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.010111 0.000585 0.231251* 0.002247 0.000111 0.000608 0.000801 0.017010 0.000898 0.000598 0.031152 0.007378
Bud Neck 0.007928 0.043837 0.049711 0.008598 0.011476 0.034565 0.003099 0.006520 0.011196 0.038791 0.009238 0.007876
Bud Site 0.349723* 0.023235 0.373044* 0.057966 0.004207 0.020098 0.007808 0.050726 0.031398 0.021527 0.055910 0.055449
Cell Periphery 0.041057 0.000163 0.082134 0.000197 0.000121 0.000206 0.000132 0.000319 0.003342 0.000186 0.010932 0.000240
Cytoplasm 0.458447* 0.364841* 0.040933 0.499580* 0.503010* 0.379690* 0.533857* 0.524830* 0.499503* 0.372923* 0.468939* 0.508357*
Cytoplasmic Foci 0.054888 0.298964* 0.062692 0.034087 0.221887* 0.245017* 0.011456 0.044842 0.208745* 0.269602* 0.018203 0.037825
Eisosomes 0.000238 0.000181 0.020172 0.000051 0.000141 0.000166 0.000037 0.000160 0.000149 0.000173 0.002688 0.000089
Endoplasmic Reticulum 0.008277 0.000547 0.014836 0.001493 0.000775 0.000744 0.001320 0.002498 0.001365 0.000654 0.003100 0.001842
Endosome 0.005569 0.032398 0.009653 0.012946 0.009368 0.036878 0.001765 0.007808 0.009069 0.034837 0.002803 0.011160
Golgi 0.007832 0.008523 0.010602 0.006582 0.001535 0.007495 0.000135 0.001224 0.002031 0.007963 0.001514 0.004719
Lipid Particles 0.011197 0.006660 0.056984 0.001777 0.010032 0.009744 0.000764 0.001843 0.010124 0.008338 0.008168 0.001800
Mitochondria 0.000993 0.012541 0.001065 0.004200 0.005042 0.016094 0.002778 0.000637 0.004723 0.014475 0.002552 0.002962
Mitotic Spindle 0.000875 0.006999 0.002589 0.043203 0.000437 0.017238 0.009908 0.021600 0.000472 0.012572 0.008944 0.035694
None 0.000280 0.002686 0.000219 0.005672 0.003573 0.003104 0.005300 0.003814 0.003314 0.002914 0.004631 0.005026
Nuclear Periphery 0.000418 0.000265 0.000308 0.000919 0.000588 0.000499 0.001271 0.000678 0.000574 0.000392 0.001145 0.000835
Nuclear Periphery Foci 0.001486 0.000503 0.002184 0.003455 0.000372 0.000409 0.000999 0.001928 0.000460 0.000452 0.001155 0.002924
Nucleolus 0.000454 0.001870 0.000036 0.000784 0.000925 0.002402 0.000309 0.000226 0.000888 0.002159 0.000273 0.000590
Nucleus 0.009578 0.083838 0.003297 0.120422 0.173974* 0.105815 0.355161* 0.164916* 0.161037* 0.095799 0.308820* 0.135888*
Peroxisomes 0.002098 0.036556 0.003474 0.002812 0.005480 0.018589 0.000335 0.003008 0.005214 0.026777 0.000748 0.002880
Vacuole 0.022690 0.073085 0.034040 0.183753 0.046196 0.097258 0.062561 0.144777 0.044346 0.086242 0.058805 0.170206
Vacuole Periphery 0.005861 0.001722 0.000776 0.009257 0.000750 0.003381 0.000204 0.000635 0.001152 0.002625 0.000280 0.006260

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.48 -13.39 4.27 -1.62 14.12 -3.36 -1.29 -2.57 -2.38 -2.39 1.52 -10.92 -2.25 -2.61 9.49
Bud Neck -12.14 -12.18 1.72 13.73 13.41 -10.14 7.11 5.35 14.07 -2.53 -14.40 1.23 5.28 19.31 5.44
Bud Site 12.38 -0.63 12.23 -0.73 21.34* -8.03 -1.99 -5.44 -1.73 -4.83 2.83 -4.84 1.17 -1.82 6.51
Cell Periphery 4.05 -2.78 4.05 1.95 9.54 -4.43 0.30 -3.96 -0.83 -3.52 3.75 -4.71 3.77 1.26 8.41
Cytoplasm 3.40 13.75 2.18 -2.95 -27.90 11.96 5.01 5.06 -3.78 1.58 14.38 10.30 8.80 -4.26 -0.98
Cytoplasmic Foci -23.54 -1.39 3.91 41.16 5.26 -2.80 37.06 29.58 33.04 -9.31 -8.67 34.16 32.60 53.25 -4.52
Eisosomes 0.75 -4.49 2.41 10.71 4.54 -2.43 12.39 1.16 2.33 -4.03 -2.20 -4.21 6.52 9.99 4.34
Endoplasmic Reticulum 3.83 -1.68 3.01 -11.63 4.45 0.20 -8.70 -5.36 -5.62 -1.87 3.40 -5.55 -4.15 -11.03 3.20
Endosome -11.73 -2.48 0.07 12.79 2.99 -12.86 9.36 4.03 14.69 -2.27 -16.11 8.10 4.40 19.64 -3.48
Golgi -0.34 -0.99 3.58 5.34 3.73 -6.36 3.66 2.33 8.00 -2.06 -8.14 1.23 0.86 7.89 -0.02
Lipid Particles 0.91 -4.93 1.93 8.88 7.07 0.31 10.20 8.86 9.48 -7.17 1.76 1.24 9.17 12.84 5.92
Mitochondria -8.56 -0.17 -1.01 7.95 -0.93 -5.69 2.83 4.68 9.49 2.34 -6.81 2.90 3.72 11.90 0.91
Mitotic Spindle -3.99 -3.98 -7.35 -4.91 -6.39 -6.95 -5.89 -3.94 1.09 -1.14 -8.10 -6.17 -8.44 -2.80 -4.54
None -3.74 0.70 -7.90 -1.79 -8.21 0.54 -1.69 0.22 -0.38 2.07 0.63 -1.25 -0.91 -1.58 0.32
Nuclear Periphery 0.36 0.13 -3.51 -18.39 -16.49 1.05 -14.18 -5.47 -8.94 10.82 2.33 -12.51 -10.59 -19.01 1.87
Nuclear Periphery Foci 2.58 -2.06 -1.27 -3.41 0.60 -0.53 -3.51 -8.96 -9.07 -3.10 -0.10 -4.30 -5.17 -5.18 -2.10
Nucleolus -3.20 1.17 -0.07 3.90 -2.19 -4.47 6.08 7.87 6.35 0.26 -5.48 6.53 3.46 6.79 -0.90
Nucleus -10.68 0.79 -18.05 -11.05 -26.36 11.22 -26.43 -0.74 -8.24 19.30 12.42 -22.53 -1.00 -13.06 21.20
Peroxisomes -13.25 -1.35 2.41 13.72 2.49 -8.99 8.15 4.39 11.77 -2.85 -14.34 7.54 6.17 18.11 -2.25
Vacuole -6.99 -2.43 -26.69 -26.12 -27.34 -12.66 -14.91 -19.88 -14.92 -13.12 -13.92 -14.65 -35.61 -29.34 -25.13
Vacuole Periphery 2.37 2.87 1.56 -1.09 -2.02 -7.38 6.69 3.12 8.43 -0.99 -5.65 5.52 -1.14 1.06 -2.63
Standard name
Human Ortholog
Description Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (25%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gdb1

Gdb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gdb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available