Standard name | |
---|---|
Human Ortholog | |
Description | Regulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 14 | 4 | – | – | 0 | – | 3 | 0 | 1 | 2 | 4 | 3 | 11 | 6 | 10 | 6 | 3 | 8 | 8 | 3 | 5 | 5 | 6 |
Bud | 27 | 11 | 3 | – | – | 6 | – | 11 | 7 | 21 | 14 | 17 | 5 | 3 | 10 | 3 | 5 | 7 | 80 | 43 | 9 | 3 | 9 | 5 |
Bud Neck | 52 | 19 | 6 | – | – | 4 | – | 5 | 4 | 7 | 4 | 4 | 7 | 13 | 12 | 11 | 12 | 10 | 142 | 74 | 23 | 8 | 3 | 7 |
Bud Site | 36 | 16 | 5 | – | – | 8 | – | 10 | 13 | 13 | 5 | 15 | 11 | 13 | 14 | 12 | 11 | 12 | – | – | – | – | – | – |
Cell Periphery | 4 | 1 | 0 | – | – | 0 | – | 1 | 7 | 2 | 0 | 0 | 0 | 1 | 3 | 1 | 4 | 5 | 1 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 306 | 149 | 29 | – | – | 30 | – | 58 | 63 | 54 | 35 | 47 | 76 | 67 | 79 | 53 | 40 | 31 | 186 | 92 | 29 | 59 | 74 | 100 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | – | 1 | – | 1 | 1 | 1 | 0 | 2 | 1 | 1 | 0 | 3 | 1 | 4 | 3 | 2 | 0 | 2 | 1 | 2 |
Endosome | 18 | 7 | 0 | – | – | 1 | – | 0 | 2 | 0 | 0 | 0 | 7 | 6 | 7 | 12 | 9 | 13 | 12 | 10 | 1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 4 | 2 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 19 | 13 | 15 | – | – | 19 | – | 69 | 100 | 87 | 81 | 102 | 2 | 1 | 0 | 9 | 5 | 11 | 14 | 10 | 7 | 6 | 10 | 12 |
Nucleus | 8 | 1 | 0 | – | – | 2 | – | 4 | 0 | 0 | 0 | 3 | 2 | 0 | 1 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | – | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 1 |
Nucleolus | 4 | 1 | 0 | – | – | 2 | – | 10 | 7 | 4 | 4 | 3 | 2 | 0 | 0 | 3 | 4 | 1 | 1 | 2 | 0 | 0 | 0 | 0 |
Peroxisomes | 34 | 22 | 0 | – | – | 7 | – | 8 | 5 | 9 | 5 | 3 | 22 | 27 | 44 | 24 | 19 | 13 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 53 | 9 | 1 | – | – | 5 | – | 19 | 10 | 16 | 1 | 9 | 24 | 13 | 39 | 42 | 26 | 22 | 23 | 14 | 6 | 4 | 1 | 1 |
Vac/Vac Membrane | 3 | 3 | 0 | – | – | 1 | – | 2 | 4 | 4 | 1 | 2 | 0 | 1 | 3 | 4 | 3 | 4 | 8 | 5 | 3 | 4 | 3 | 3 |
Unique Cell Count | 473 | 235 | 48 | 63 | 136 | 155 | 153 | 120 | 146 | 129 | 127 | 165 | 139 | 112 | 104 | 498 | 269 | 88 | 102 | 115 | 148 | |||
Labelled Cell Count | 573 | 266 | 63 | 86 | 202 | 223 | 220 | 152 | 211 | 162 | 158 | 218 | 195 | 146 | 141 | 498 | 269 | 88 | 102 | 115 | 148 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 5.4 | 3.5 | – | – | 3.8 | – | 4.0 | 3.4 | 3.5 | 3.3 | 3.3 | 5.3 | 5.4 | 5.6 | 7.7 | 7.8 | 7.6 | 5.2 | 5.8 | 5.6 |
Std Deviation (1e-4) | 2.0 | 0.9 | 0.6 | – | – | 1.4 | – | 2.1 | 1.4 | 0.8 | 0.7 | 0.7 | 1.7 | 1.8 | 2.5 | 1.6 | 1.8 | 1.4 | 1.7 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | – | – | 0.15 | – | 0.23 | -0.02 | 0.0 | -0.07 | -0.06 | 0.62 | 0.65 | 0.71 | 1.16 | 1.16 | 1.14 | 0.59 | 0.75 | 0.68 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | 0 | 0 | -0.3 | -0.5 |
Bud Site | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 |
Cell Periphery | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | – | -1.3 | – | -2.1 | -2.4 | -3.1 | -3.8 | -3.5 | -0.2 | -0.9 | -1.5 | -2.7 | -2.9 | -3.6 |
Endoplasmic Reticulum | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.4 | -4.7 | -3.1 |
Nucleus | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.1 | 3.5 | 4.0 | 3.1 | 3.0 | 0 |
SpindlePole | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 3.4 | 4.0 | 3.3 | 3.0 |
Vacuole | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1263 | 0.5853 | 0.1085 | 1.2532 | -0.5605 | 0.2838 | -0.1456 | -0.3336 | -0.1256 | -0.4123 | -0.1784 | -0.2678 | 0.816 | 1.1791 | 1.0642 | 1.16 | 0.6495 | 0.9815 |
Actin | 0.0078 | 0.0017 | 0.0181 | 0.1724 | 0.0001 | 0.0061 | 0.0352 | 0.0012 | 0.0171 | 0.0403 | 0.0205 | 0.0067 | 0.0093 | 0.0014 | 0.0016 | 0.0001 | 0.0002 | 0.001 |
Bud | 0.0011 | 0.004 | 0.0016 | 0.0019 | 0 | 0.0014 | 0.008 | 0.0152 | 0.0075 | 0.0005 | 0.0045 | 0.0016 | 0.0072 | 0.0044 | 0.0021 | 0.0001 | 0.0027 | 0.0013 |
Bud Neck | 0.0134 | 0.0368 | 0.581 | 0.7353 | 0.9985 | 0.411 | 0.0388 | 0.0496 | 0.5535 | 0.8374 | 0.6183 | 0.3927 | 0.036 | 0.0339 | 0.614 | 0.792 | 0.7153 | 0.4446 |
Bud Periphery | 0.0009 | 0.0036 | 0.0019 | 0.0023 | 0 | 0.0035 | 0.0108 | 0.0057 | 0.005 | 0.001 | 0.0091 | 0.0019 | 0.0086 | 0.0052 | 0.0044 | 0.0001 | 0.0058 | 0.0017 |
Bud Site | 0.1018 | 0.3557 | 0.131 | 0.0151 | 0.0001 | 0.0048 | 0.1702 | 0.3565 | 0.195 | 0.0232 | 0.0153 | 0.0045 | 0.1501 | 0.2659 | 0.165 | 0.0013 | 0.0084 | 0.0016 |
Cell Periphery | 0.0008 | 0.0026 | 0.0015 | 0.0009 | 0.0001 | 0.0007 | 0.0021 | 0.0014 | 0.0022 | 0.0006 | 0.0009 | 0.0004 | 0.001 | 0.0016 | 0.0011 | 0.0001 | 0.0002 | 0.0003 |
Cytoplasm | 0.1716 | 0.092 | 0.0198 | 0.0008 | 0.0002 | 0.3168 | 0.122 | 0.106 | 0.0135 | 0.0051 | 0.0254 | 0.1693 | 0.1723 | 0.134 | 0.0244 | 0.0858 | 0.0071 | 0.1438 |
Cytoplasmic Foci | 0.0585 | 0.0516 | 0.0247 | 0.0138 | 0.0007 | 0.02 | 0.126 | 0.0277 | 0.0293 | 0.006 | 0.0924 | 0.0215 | 0.0823 | 0.0214 | 0.0138 | 0.0109 | 0.0594 | 0.0352 |
Eisosomes | 0.0006 | 0.0005 | 0.0006 | 0.0006 | 0 | 0.0001 | 0.0005 | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0005 | 0.0004 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0017 | 0.0005 | 0.0004 | 0.0007 | 0 | 0.007 | 0.0046 | 0.0007 | 0.0006 | 0.0002 | 0.0013 | 0.0007 | 0.0024 | 0.001 | 0.0002 | 0.0003 | 0.0001 | 0.0005 |
Endosome | 0.0082 | 0.001 | 0.0016 | 0.0063 | 0 | 0.012 | 0.0285 | 0.0035 | 0.0053 | 0.0124 | 0.029 | 0.0069 | 0.008 | 0.0034 | 0.0003 | 0.0018 | 0.0005 | 0.0024 |
Golgi | 0.0023 | 0.0006 | 0.0012 | 0.0135 | 0 | 0.0008 | 0.0113 | 0.0005 | 0.0068 | 0.0047 | 0.0215 | 0.0091 | 0.0019 | 0.0008 | 0.0003 | 0.0002 | 0.0002 | 0.0004 |
Lipid Particles | 0.0074 | 0.002 | 0.0057 | 0.0108 | 0 | 0.0006 | 0.0227 | 0.0009 | 0.0033 | 0.0211 | 0.02 | 0.0045 | 0.0024 | 0.0018 | 0.0003 | 0.0001 | 0.0002 | 0.0007 |
Mitochondria | 0.0013 | 0.0011 | 0.0008 | 0.0054 | 0 | 0.0008 | 0.0097 | 0.0006 | 0.013 | 0.0006 | 0.0095 | 0.0018 | 0.0008 | 0.0009 | 0.0003 | 0.0001 | 0.0002 | 0.0004 |
None | 0.5994 | 0.4197 | 0.1976 | 0.0005 | 0 | 0.2101 | 0.3487 | 0.3937 | 0.1205 | 0.0421 | 0.0687 | 0.3525 | 0.4972 | 0.4851 | 0.1534 | 0.1046 | 0.093 | 0.3488 |
Nuclear Periphery | 0.0004 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0004 | 0.0039 | 0.0005 | 0.0004 | 0.0002 | 0.0019 | 0.0007 | 0.0029 | 0.0005 | 0.0001 | 0.0002 | 0.0007 | 0.0007 |
Nucleolus | 0.0015 | 0.0097 | 0.0017 | 0.0018 | 0.0001 | 0.0005 | 0.0021 | 0.0062 | 0.0031 | 0.0015 | 0.0048 | 0.0009 | 0.0014 | 0.0033 | 0.0023 | 0.0002 | 0.0023 | 0.0015 |
Nucleus | 0.0006 | 0.0048 | 0.001 | 0.0006 | 0.0001 | 0.0005 | 0.002 | 0.0069 | 0.0029 | 0.0007 | 0.0067 | 0.0013 | 0.0019 | 0.0105 | 0.0016 | 0.0008 | 0.0101 | 0.0028 |
Peroxisomes | 0.015 | 0.0022 | 0.0058 | 0.0121 | 0 | 0.0005 | 0.0341 | 0.0013 | 0.0135 | 0.0009 | 0.0314 | 0.0098 | 0.0038 | 0.0019 | 0.001 | 0.0001 | 0.0004 | 0.0012 |
Punctate Nuclear | 0.0039 | 0.0085 | 0.0033 | 0.0024 | 0.0001 | 0.0006 | 0.0141 | 0.0164 | 0.0048 | 0.0006 | 0.0142 | 0.0119 | 0.007 | 0.0181 | 0.0132 | 0.0005 | 0.0929 | 0.0098 |
Vacuole | 0.0018 | 0.001 | 0.0006 | 0.0017 | 0 | 0.0013 | 0.004 | 0.0049 | 0.0016 | 0.0008 | 0.0034 | 0.0007 | 0.0025 | 0.0044 | 0.0005 | 0.0007 | 0.0003 | 0.001 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0011 | 0.0003 | 0.0002 | 0.0002 | 0.0008 | 0.0002 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 42.6111 | 52.1256 | 41.6548 | 37.7786 | 34.893 | 47.0705 | 36.9942 | 47.885 | 48.2058 | 46.7392 |
Translational Efficiency | 2.003 | 1.4951 | 1.7454 | 2.1646 | 2.2373 | 1.743 | 2.1366 | 1.508 | 1.5967 | 1.753 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2099 | 1224 | 94 | 1635 | 2310 | 127 | 1901 | 1216 | 4409 | 1351 | 1995 | 2851 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 695.59 | 719.29 | 922.83 | 868.40 | 668.65 | 771.48 | 767.43 | 835.38 | 681.48 | 724.20 | 774.75 | 854.32 |
Standard Deviation | 82.41 | 91.62 | 104.99 | 130.56 | 74.39 | 102.03 | 140.17 | 130.12 | 79.45 | 93.89 | 142.56 | 131.39 |
Intensity Change Log 2 | — | 0.048336 | 0.407828 | 0.320123 | — | 0.206378 | 0.198784 | 0.321181 | — | 0.127960 | 0.309148 | 0.320642 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000485 | 0.000762 | 0.001148 | 0.004752 | 0.000545 | 0.000217 | 0.000650 | 0.008014 | 0.000517 | 0.000711 | 0.000674 | 0.006143 |
Bud Neck | 0.331456* | 0.200663* | 0.198345* | 0.165560 | 0.298042* | 0.220387* | 0.222063* | 0.166128 | 0.313950* | 0.202517* | 0.220945* | 0.165802 |
Bud Site | 0.119502* | 0.169286* | 0.195982* | 0.502983* | 0.084652 | 0.038327 | 0.361482* | 0.478309* | 0.101243* | 0.156975* | 0.353684* | 0.492459* |
Cell Periphery | 0.000371 | 0.000410 | 0.000342 | 0.000883 | 0.000386 | 0.000422 | 0.000459 | 0.000558 | 0.000379 | 0.000412 | 0.000454 | 0.000745 |
Cytoplasm | 0.290620* | 0.246517* | 0.163074* | 0.160442* | 0.305623* | 0.205377* | 0.238230* | 0.157047* | 0.298480* | 0.242649* | 0.234689* | 0.158994* |
Cytoplasmic Foci | 0.107312 | 0.164584 | 0.045576 | 0.025440 | 0.140280 | 0.223216* | 0.019725 | 0.032404 | 0.124585 | 0.170095 | 0.020943 | 0.028410 |
Eisosomes | 0.000231 | 0.000153 | 0.000177 | 0.000135 | 0.000216 | 0.000304 | 0.000084 | 0.000118 | 0.000223 | 0.000167 | 0.000089 | 0.000128 |
Endoplasmic Reticulum | 0.000669 | 0.000447 | 0.003656 | 0.000378 | 0.000624 | 0.000528 | 0.000564 | 0.000416 | 0.000646 | 0.000455 | 0.000710 | 0.000394 |
Endosome | 0.018228 | 0.056209 | 0.021258 | 0.007669 | 0.024818 | 0.099178 | 0.010136 | 0.008956 | 0.021681 | 0.060249 | 0.010660 | 0.008218 |
Golgi | 0.007022 | 0.015085 | 0.001095 | 0.003383 | 0.005224 | 0.013988 | 0.003207 | 0.003808 | 0.006080 | 0.014982 | 0.003108 | 0.003565 |
Lipid Particles | 0.003446 | 0.003976 | 0.003985 | 0.000457 | 0.004896 | 0.022006 | 0.000493 | 0.000425 | 0.004206 | 0.005671 | 0.000658 | 0.000443 |
Mitochondria | 0.012033 | 0.016508 | 0.000342 | 0.006176 | 0.011938 | 0.010869 | 0.001217 | 0.002128 | 0.011983 | 0.015978 | 0.001175 | 0.004449 |
Mitotic Spindle | 0.001902 | 0.017426 | 0.006286 | 0.026483 | 0.003245 | 0.002612 | 0.016241 | 0.031659 | 0.002605 | 0.016033 | 0.015772 | 0.028690 |
None | 0.003381 | 0.003687 | 0.001830 | 0.000483 | 0.001563 | 0.000486 | 0.003704 | 0.000731 | 0.002429 | 0.003386 | 0.003616 | 0.000589 |
Nuclear Periphery | 0.000067 | 0.000102 | 0.000774 | 0.000122 | 0.000129 | 0.000837 | 0.000230 | 0.000108 | 0.000099 | 0.000171 | 0.000256 | 0.000116 |
Nuclear Periphery Foci | 0.000135 | 0.000418 | 0.005270 | 0.000454 | 0.000153 | 0.000291 | 0.000717 | 0.000333 | 0.000144 | 0.000406 | 0.000932 | 0.000403 |
Nucleolus | 0.001958 | 0.002024 | 0.002138 | 0.000495 | 0.001495 | 0.001047 | 0.002894 | 0.000950 | 0.001716 | 0.001932 | 0.002858 | 0.000689 |
Nucleus | 0.045934 | 0.017764 | 0.158557* | 0.010992 | 0.051049 | 0.043558 | 0.064656 | 0.018981 | 0.048614 | 0.020189 | 0.069080 | 0.014399 |
Peroxisomes | 0.010781 | 0.030872 | 0.018717 | 0.035166 | 0.013390 | 0.042485 | 0.012604 | 0.044344 | 0.012148 | 0.031964 | 0.012892 | 0.039081 |
Vacuole | 0.042889 | 0.049549 | 0.170396 | 0.045659 | 0.050398 | 0.066564 | 0.039878 | 0.044023 | 0.046823 | 0.051149 | 0.046028 | 0.044961 |
Vacuole Periphery | 0.001576 | 0.003559 | 0.001054 | 0.001886 | 0.001334 | 0.007301 | 0.000765 | 0.000560 | 0.001449 | 0.003910 | 0.000779 | 0.001320 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.97 | -2.24 | -7.47 | -6.96 | -4.93 | 3.18 | -1.56 | -6.83 | -7.12 | -6.29 | -1.68 | -1.85 | -9.91 | -9.47 | -8.41 |
Bud Neck | 12.26 | 7.48 | 21.20 | 5.90 | -0.12 | 2.56 | 10.74 | 16.49 | 2.58 | 6.94 | 12.02 | 15.76 | 26.39 | 6.80 | 7.82 |
Bud Site | -6.79 | -1.27 | -37.38* | -27.09* | -12.20 | 5.71 | -31.18* | -33.70* | -31.40* | -8.02 | -8.73 | -29.65 | -50.94 | -32.60* | -13.12 |
Cell Periphery | -0.71 | 0.37 | -1.64 | -1.49 | -1.71 | -0.43 | -0.66 | -1.96 | -1.06 | -1.39 | -0.69 | -0.87 | -1.97 | -1.73 | -1.69 |
Cytoplasm | 4.33 | 5.51 | 11.13 | 5.36 | -1.15 | 4.62 | 5.46 | 12.61 | 0.86 | 6.87 | 6.40 | 5.96 | 16.88 | 6.00 | 7.42 |
Cytoplasmic Foci | -8.41 | 9.23 | 20.10 | 21.83 | 1.96 | -4.59 | 29.87 | 23.26 | 10.51 | -5.60 | -7.40 | 36.31 | 31.10 | 23.75 | -5.55 |
Eisosomes | 3.33 | 1.27 | 4.01 | 0.75 | 2.80 | -2.03 | 10.05 | 5.63 | 4.12 | -4.31 | 3.28 | 11.16 | 6.35 | 2.08 | -4.68 |
Endoplasmic Reticulum | 1.43 | -5.65 | 1.25 | -0.45 | 5.86 | 0.35 | -1.10 | -0.45 | -1.16 | 1.52 | 1.18 | -3.16 | 0.32 | -1.52 | 6.06 |
Endosome | -12.37 | -0.49 | 9.13 | 16.33 | 5.21 | -5.47 | 10.62 | 10.32 | 6.70 | 1.05 | -12.60 | 11.31 | 13.98 | 17.49 | 2.56 |
Golgi | -6.71 | 12.93 | 6.99 | 10.31 | -4.88 | -2.45 | 4.58 | 2.49 | 3.01 | -0.51 | -7.98 | 8.66 | 6.27 | 10.64 | -0.70 |
Lipid Particles | -1.04 | -1.81 | 8.46 | 6.58 | 6.03 | -2.85 | 9.26 | 9.13 | 3.57 | -0.53 | -1.93 | 11.69 | 12.21 | 6.86 | 2.03 |
Mitochondria | -2.31 | 13.93 | 4.75 | 5.42 | -4.36 | 0.41 | 9.55 | 8.67 | 3.82 | -1.94 | -2.29 | 14.92 | 8.36 | 7.10 | -3.93 |
Mitotic Spindle | -6.74 | -1.49 | -10.37 | -3.07 | -6.30 | 0.49 | -8.46 | -8.28 | -8.21 | -3.64 | -6.37 | -9.32 | -13.07 | -4.58 | -4.75 |
None | -0.23 | 2.98 | 7.47 | 2.38 | 1.36 | 4.86 | -3.64 | 2.94 | -1.85 | 4.97 | -0.83 | -1.99 | 7.92 | 2.31 | 5.33 |
Nuclear Periphery | -2.59 | -8.48 | -7.33 | -5.06 | 6.87 | -2.26 | -8.72 | -4.26 | 1.91 | 4.90 | -2.22 | -11.13 | -7.93 | -1.87 | 6.17 |
Nuclear Periphery Foci | -3.56 | -5.26 | -4.70 | -0.46 | 4.91 | -1.82 | -7.55 | -5.43 | -2.74 | 4.13 | -3.47 | -9.41 | -6.53 | -0.61 | 5.60 |
Nucleolus | -0.04 | -0.16 | 3.39 | 2.96 | 2.32 | 1.31 | -3.24 | 0.92 | -0.10 | 4.08 | -0.28 | -3.31 | 2.95 | 2.49 | 5.73 |
Nucleus | 10.51 | -5.82 | 10.07 | -0.72 | 6.90 | 0.94 | -8.67 | 4.40 | 0.94 | 10.87 | 12.40 | -10.69 | 10.39 | -2.47 | 15.42 |
Peroxisomes | -9.03 | -0.81 | -8.20 | -0.84 | -4.54 | -3.38 | 1.05 | -8.08 | 0.16 | -8.53 | -9.19 | 0.10 | -11.35 | -1.67 | -10.90 |
Vacuole | -2.32 | -7.60 | -8.76 | -6.74 | 5.98 | -1.65 | -2.91 | -7.25 | -2.41 | -5.15 | -1.70 | -6.62 | -11.16 | -8.50 | -3.98 |
Vacuole Periphery | -5.58 | 1.68 | -0.10 | 3.57 | -1.29 | -2.04 | 4.15 | 6.96 | 2.32 | 1.35 | -5.90 | 5.32 | 1.18 | 5.76 | -1.58 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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