Standard name
Human Ortholog
Description Regulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0.08 – – 0 – 0 0 0 0 0 0 0.09 0 0.07 0.05 0 0 0 0 0.06 0.05 0
Bud 0.06 0.05 0.06 – – 0.1 – 0.08 0.05 0.14 0.12 0.12 0 0 0.06 0 0 0.07 0.16 0.16 0.11 0 0.08 0
Bud Neck 0.11 0.08 0.12 – – 0.06 – 0 0 0.05 0 0 0.05 0.1 0.07 0.08 0.11 0.1 0.29 0.28 0.27 0.09 0 0.05
Bud Site 0.08 0.07 0.1 – – 0.13 – 0.07 0.08 0.08 0 0.1 0.09 0.1 0.08 0.09 0.1 0.12 – – – – – –
Cell Periphery 0 0 0 – – 0 – 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.65 0.63 0.6 – – 0.48 – 0.43 0.41 0.35 0.29 0.32 0.59 0.53 0.48 0.38 0.36 0.3 0.37 0.34 0.34 0.59 0.65 0.68
Endoplasmic Reticulum 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – – 0 – 0 0 0 0 0 0.05 0.05 0 0.09 0.08 0.12 0 0 0 0 0 0
Golgi 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0.31 – – 0.3 – 0.51 0.65 0.57 0.68 0.7 0 0 0 0.06 0 0.11 0 0 0.09 0.06 0.1 0.08
Nucleus 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – – 0 – 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0.09 0 – – 0.11 – 0.06 0 0.06 0 0 0.17 0.21 0.27 0.17 0.17 0.12 0 0 0 0 0 0
SpindlePole 0.11 0 0 – – 0.08 – 0.14 0.06 0.1 0 0.06 0.19 0.1 0.24 0.3 0.23 0.21 0.05 0.05 0.08 0.05 0 0
Vac/Vac Membrane 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 14 4 – – 0 – 3 0 1 2 4 3 11 6 10 6 3 8 8 3 5 5 6
Bud 27 11 3 – – 6 – 11 7 21 14 17 5 3 10 3 5 7 80 43 9 3 9 5
Bud Neck 52 19 6 – – 4 – 5 4 7 4 4 7 13 12 11 12 10 142 74 23 8 3 7
Bud Site 36 16 5 – – 8 – 10 13 13 5 15 11 13 14 12 11 12 – – – – – –
Cell Periphery 4 1 0 – – 0 – 1 7 2 0 0 0 1 3 1 4 5 1 0 0 0 0 1
Cytoplasm 306 149 29 – – 30 – 58 63 54 35 47 76 67 79 53 40 31 186 92 29 59 74 100
Endoplasmic Reticulum 0 0 0 – – 1 – 1 1 1 0 2 1 1 0 3 1 4 3 2 0 2 1 2
Endosome 18 7 0 – – 1 – 0 2 0 0 0 7 6 7 12 9 13 12 10 1 0 0 0
Golgi 0 0 0 – – 0 – 0 0 0 0 0 0 0 0 4 0 4 2 1 0 0 1 0
Mitochondria 19 13 15 – – 19 – 69 100 87 81 102 2 1 0 9 5 11 14 10 7 6 10 12
Nucleus 8 1 0 – – 2 – 4 0 0 0 3 2 0 1 4 1 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – – 0 – 1 0 1 0 0 0 1 0 0 0 0 2 0 0 2 0 1
Nucleolus 4 1 0 – – 2 – 10 7 4 4 3 2 0 0 3 4 1 1 2 0 0 0 0
Peroxisomes 34 22 0 – – 7 – 8 5 9 5 3 22 27 44 24 19 13 0 1 0 0 0 0
SpindlePole 53 9 1 – – 5 – 19 10 16 1 9 24 13 39 42 26 22 23 14 6 4 1 1
Vac/Vac Membrane 3 3 0 – – 1 – 2 4 4 1 2 0 1 3 4 3 4 8 5 3 4 3 3
Unique Cell Count 473 235 48 63 136 155 153 120 146 129 127 165 139 112 104 498 269 88 102 115 148
Labelled Cell Count 573 266 63 86 202 223 220 152 211 162 158 218 195 146 141 498 269 88 102 115 148


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.4 3.5 – – 3.8 – 4.0 3.4 3.5 3.3 3.3 5.3 5.4 5.6 7.7 7.8 7.6 5.2 5.8 5.6
Std Deviation (1e-4) 2.0 0.9 0.6 – – 1.4 – 2.1 1.4 0.8 0.7 0.7 1.7 1.8 2.5 1.6 1.8 1.4 1.7 1.8 1.8
Intensity Change (Log2) – – – – – 0.15 – 0.23 -0.02 0.0 -0.07 -0.06 0.62 0.65 0.71 1.16 1.16 1.14 0.59 0.75 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – – 0 – 0 0 0 0 0 0 -0.4 0 0 -0.3 -0.5
Bud Site – – 0 – 0 0 0 0 0 0 0 0 0 0 0.2
Cell Periphery – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – – -1.3 – -2.1 -2.4 -3.1 -3.8 -3.5 -0.2 -0.9 -1.5 -2.7 -2.9 -3.6
Endoplasmic Reticulum – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – – 0 – 0 0 0 0 0 0 0 0 -4.4 -4.7 -3.1
Nucleus – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – – 0 – 0 0 0 0 0 3.1 3.5 4.0 3.1 3.0 0
SpindlePole – – 0 – 0 0 0 0 0 2.8 0 3.4 4.0 3.3 3.0
Vacuole – – 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.1263 0.5853 0.1085 1.2532 -0.5605 0.2838 -0.1456 -0.3336 -0.1256 -0.4123 -0.1784 -0.2678 0.816 1.1791 1.0642 1.16 0.6495 0.9815
Actin 0.0078 0.0017 0.0181 0.1724 0.0001 0.0061 0.0352 0.0012 0.0171 0.0403 0.0205 0.0067 0.0093 0.0014 0.0016 0.0001 0.0002 0.001
Bud 0.0011 0.004 0.0016 0.0019 0 0.0014 0.008 0.0152 0.0075 0.0005 0.0045 0.0016 0.0072 0.0044 0.0021 0.0001 0.0027 0.0013
Bud Neck 0.0134 0.0368 0.581 0.7353 0.9985 0.411 0.0388 0.0496 0.5535 0.8374 0.6183 0.3927 0.036 0.0339 0.614 0.792 0.7153 0.4446
Bud Periphery 0.0009 0.0036 0.0019 0.0023 0 0.0035 0.0108 0.0057 0.005 0.001 0.0091 0.0019 0.0086 0.0052 0.0044 0.0001 0.0058 0.0017
Bud Site 0.1018 0.3557 0.131 0.0151 0.0001 0.0048 0.1702 0.3565 0.195 0.0232 0.0153 0.0045 0.1501 0.2659 0.165 0.0013 0.0084 0.0016
Cell Periphery 0.0008 0.0026 0.0015 0.0009 0.0001 0.0007 0.0021 0.0014 0.0022 0.0006 0.0009 0.0004 0.001 0.0016 0.0011 0.0001 0.0002 0.0003
Cytoplasm 0.1716 0.092 0.0198 0.0008 0.0002 0.3168 0.122 0.106 0.0135 0.0051 0.0254 0.1693 0.1723 0.134 0.0244 0.0858 0.0071 0.1438
Cytoplasmic Foci 0.0585 0.0516 0.0247 0.0138 0.0007 0.02 0.126 0.0277 0.0293 0.006 0.0924 0.0215 0.0823 0.0214 0.0138 0.0109 0.0594 0.0352
Eisosomes 0.0006 0.0005 0.0006 0.0006 0 0.0001 0.0005 0.0003 0.0006 0.0001 0.0004 0.0002 0.0005 0.0004 0.0002 0 0 0
Endoplasmic Reticulum 0.0017 0.0005 0.0004 0.0007 0 0.007 0.0046 0.0007 0.0006 0.0002 0.0013 0.0007 0.0024 0.001 0.0002 0.0003 0.0001 0.0005
Endosome 0.0082 0.001 0.0016 0.0063 0 0.012 0.0285 0.0035 0.0053 0.0124 0.029 0.0069 0.008 0.0034 0.0003 0.0018 0.0005 0.0024
Golgi 0.0023 0.0006 0.0012 0.0135 0 0.0008 0.0113 0.0005 0.0068 0.0047 0.0215 0.0091 0.0019 0.0008 0.0003 0.0002 0.0002 0.0004
Lipid Particles 0.0074 0.002 0.0057 0.0108 0 0.0006 0.0227 0.0009 0.0033 0.0211 0.02 0.0045 0.0024 0.0018 0.0003 0.0001 0.0002 0.0007
Mitochondria 0.0013 0.0011 0.0008 0.0054 0 0.0008 0.0097 0.0006 0.013 0.0006 0.0095 0.0018 0.0008 0.0009 0.0003 0.0001 0.0002 0.0004
None 0.5994 0.4197 0.1976 0.0005 0 0.2101 0.3487 0.3937 0.1205 0.0421 0.0687 0.3525 0.4972 0.4851 0.1534 0.1046 0.093 0.3488
Nuclear Periphery 0.0004 0.0001 0.0001 0.0008 0 0.0004 0.0039 0.0005 0.0004 0.0002 0.0019 0.0007 0.0029 0.0005 0.0001 0.0002 0.0007 0.0007
Nucleolus 0.0015 0.0097 0.0017 0.0018 0.0001 0.0005 0.0021 0.0062 0.0031 0.0015 0.0048 0.0009 0.0014 0.0033 0.0023 0.0002 0.0023 0.0015
Nucleus 0.0006 0.0048 0.001 0.0006 0.0001 0.0005 0.002 0.0069 0.0029 0.0007 0.0067 0.0013 0.0019 0.0105 0.0016 0.0008 0.0101 0.0028
Peroxisomes 0.015 0.0022 0.0058 0.0121 0 0.0005 0.0341 0.0013 0.0135 0.0009 0.0314 0.0098 0.0038 0.0019 0.001 0.0001 0.0004 0.0012
Punctate Nuclear 0.0039 0.0085 0.0033 0.0024 0.0001 0.0006 0.0141 0.0164 0.0048 0.0006 0.0142 0.0119 0.007 0.0181 0.0132 0.0005 0.0929 0.0098
Vacuole 0.0018 0.001 0.0006 0.0017 0 0.0013 0.004 0.0049 0.0016 0.0008 0.0034 0.0007 0.0025 0.0044 0.0005 0.0007 0.0003 0.001
Vacuole Periphery 0.0002 0.0001 0.0001 0.0003 0 0.0003 0.0011 0.0003 0.0002 0.0002 0.0008 0.0002 0.0003 0.0003 0 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.6111 52.1256 41.6548 37.7786 34.893 47.0705 36.9942 47.885 48.2058 46.7392
Translational Efficiency 2.003 1.4951 1.7454 2.1646 2.2373 1.743 2.1366 1.508 1.5967 1.753

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2099 1224 94 1635 2310 127 1901 1216 4409 1351 1995 2851

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 695.59 719.29 922.83 868.40 668.65 771.48 767.43 835.38 681.48 724.20 774.75 854.32
Standard Deviation 82.41 91.62 104.99 130.56 74.39 102.03 140.17 130.12 79.45 93.89 142.56 131.39
Intensity Change Log 2 — 0.048336 0.407828 0.320123 — 0.206378 0.198784 0.321181 — 0.127960 0.309148 0.320642

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000485 0.000762 0.001148 0.004752 0.000545 0.000217 0.000650 0.008014 0.000517 0.000711 0.000674 0.006143
Bud Neck 0.331456* 0.200663* 0.198345* 0.165560 0.298042* 0.220387* 0.222063* 0.166128 0.313950* 0.202517* 0.220945* 0.165802
Bud Site 0.119502* 0.169286* 0.195982* 0.502983* 0.084652 0.038327 0.361482* 0.478309* 0.101243* 0.156975* 0.353684* 0.492459*
Cell Periphery 0.000371 0.000410 0.000342 0.000883 0.000386 0.000422 0.000459 0.000558 0.000379 0.000412 0.000454 0.000745
Cytoplasm 0.290620* 0.246517* 0.163074* 0.160442* 0.305623* 0.205377* 0.238230* 0.157047* 0.298480* 0.242649* 0.234689* 0.158994*
Cytoplasmic Foci 0.107312 0.164584 0.045576 0.025440 0.140280 0.223216* 0.019725 0.032404 0.124585 0.170095 0.020943 0.028410
Eisosomes 0.000231 0.000153 0.000177 0.000135 0.000216 0.000304 0.000084 0.000118 0.000223 0.000167 0.000089 0.000128
Endoplasmic Reticulum 0.000669 0.000447 0.003656 0.000378 0.000624 0.000528 0.000564 0.000416 0.000646 0.000455 0.000710 0.000394
Endosome 0.018228 0.056209 0.021258 0.007669 0.024818 0.099178 0.010136 0.008956 0.021681 0.060249 0.010660 0.008218
Golgi 0.007022 0.015085 0.001095 0.003383 0.005224 0.013988 0.003207 0.003808 0.006080 0.014982 0.003108 0.003565
Lipid Particles 0.003446 0.003976 0.003985 0.000457 0.004896 0.022006 0.000493 0.000425 0.004206 0.005671 0.000658 0.000443
Mitochondria 0.012033 0.016508 0.000342 0.006176 0.011938 0.010869 0.001217 0.002128 0.011983 0.015978 0.001175 0.004449
Mitotic Spindle 0.001902 0.017426 0.006286 0.026483 0.003245 0.002612 0.016241 0.031659 0.002605 0.016033 0.015772 0.028690
None 0.003381 0.003687 0.001830 0.000483 0.001563 0.000486 0.003704 0.000731 0.002429 0.003386 0.003616 0.000589
Nuclear Periphery 0.000067 0.000102 0.000774 0.000122 0.000129 0.000837 0.000230 0.000108 0.000099 0.000171 0.000256 0.000116
Nuclear Periphery Foci 0.000135 0.000418 0.005270 0.000454 0.000153 0.000291 0.000717 0.000333 0.000144 0.000406 0.000932 0.000403
Nucleolus 0.001958 0.002024 0.002138 0.000495 0.001495 0.001047 0.002894 0.000950 0.001716 0.001932 0.002858 0.000689
Nucleus 0.045934 0.017764 0.158557* 0.010992 0.051049 0.043558 0.064656 0.018981 0.048614 0.020189 0.069080 0.014399
Peroxisomes 0.010781 0.030872 0.018717 0.035166 0.013390 0.042485 0.012604 0.044344 0.012148 0.031964 0.012892 0.039081
Vacuole 0.042889 0.049549 0.170396 0.045659 0.050398 0.066564 0.039878 0.044023 0.046823 0.051149 0.046028 0.044961
Vacuole Periphery 0.001576 0.003559 0.001054 0.001886 0.001334 0.007301 0.000765 0.000560 0.001449 0.003910 0.000779 0.001320

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.97 -2.24 -7.47 -6.96 -4.93 3.18 -1.56 -6.83 -7.12 -6.29 -1.68 -1.85 -9.91 -9.47 -8.41
Bud Neck 12.26 7.48 21.20 5.90 -0.12 2.56 10.74 16.49 2.58 6.94 12.02 15.76 26.39 6.80 7.82
Bud Site -6.79 -1.27 -37.38* -27.09* -12.20 5.71 -31.18* -33.70* -31.40* -8.02 -8.73 -29.65 -50.94 -32.60* -13.12
Cell Periphery -0.71 0.37 -1.64 -1.49 -1.71 -0.43 -0.66 -1.96 -1.06 -1.39 -0.69 -0.87 -1.97 -1.73 -1.69
Cytoplasm 4.33 5.51 11.13 5.36 -1.15 4.62 5.46 12.61 0.86 6.87 6.40 5.96 16.88 6.00 7.42
Cytoplasmic Foci -8.41 9.23 20.10 21.83 1.96 -4.59 29.87 23.26 10.51 -5.60 -7.40 36.31 31.10 23.75 -5.55
Eisosomes 3.33 1.27 4.01 0.75 2.80 -2.03 10.05 5.63 4.12 -4.31 3.28 11.16 6.35 2.08 -4.68
Endoplasmic Reticulum 1.43 -5.65 1.25 -0.45 5.86 0.35 -1.10 -0.45 -1.16 1.52 1.18 -3.16 0.32 -1.52 6.06
Endosome -12.37 -0.49 9.13 16.33 5.21 -5.47 10.62 10.32 6.70 1.05 -12.60 11.31 13.98 17.49 2.56
Golgi -6.71 12.93 6.99 10.31 -4.88 -2.45 4.58 2.49 3.01 -0.51 -7.98 8.66 6.27 10.64 -0.70
Lipid Particles -1.04 -1.81 8.46 6.58 6.03 -2.85 9.26 9.13 3.57 -0.53 -1.93 11.69 12.21 6.86 2.03
Mitochondria -2.31 13.93 4.75 5.42 -4.36 0.41 9.55 8.67 3.82 -1.94 -2.29 14.92 8.36 7.10 -3.93
Mitotic Spindle -6.74 -1.49 -10.37 -3.07 -6.30 0.49 -8.46 -8.28 -8.21 -3.64 -6.37 -9.32 -13.07 -4.58 -4.75
None -0.23 2.98 7.47 2.38 1.36 4.86 -3.64 2.94 -1.85 4.97 -0.83 -1.99 7.92 2.31 5.33
Nuclear Periphery -2.59 -8.48 -7.33 -5.06 6.87 -2.26 -8.72 -4.26 1.91 4.90 -2.22 -11.13 -7.93 -1.87 6.17
Nuclear Periphery Foci -3.56 -5.26 -4.70 -0.46 4.91 -1.82 -7.55 -5.43 -2.74 4.13 -3.47 -9.41 -6.53 -0.61 5.60
Nucleolus -0.04 -0.16 3.39 2.96 2.32 1.31 -3.24 0.92 -0.10 4.08 -0.28 -3.31 2.95 2.49 5.73
Nucleus 10.51 -5.82 10.07 -0.72 6.90 0.94 -8.67 4.40 0.94 10.87 12.40 -10.69 10.39 -2.47 15.42
Peroxisomes -9.03 -0.81 -8.20 -0.84 -4.54 -3.38 1.05 -8.08 0.16 -8.53 -9.19 0.10 -11.35 -1.67 -10.90
Vacuole -2.32 -7.60 -8.76 -6.74 5.98 -1.65 -2.91 -7.25 -2.41 -5.15 -1.70 -6.62 -11.16 -8.50 -3.98
Vacuole Periphery -5.58 1.68 -0.10 3.57 -1.29 -2.04 4.15 6.96 2.32 1.35 -5.90 5.32 1.18 5.76 -1.58
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Regulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
Localization
Cell Percentages cytoplasm (25%), bud neck (25%), mixed (19%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (3.3e-18)
bud neck - S/G2 (7.4e-07)
bud neck - MA (1.2e-09)
Subcompartmental Group N/A

Mlc2

Mlc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mlc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available