Standard name
Human Ortholog
Description RNA polymerase III subunit C82
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.55 0.45 0.82 0.81 0.83 0.84 0.82 0.76 0.72 0.74 0.58 0.71 0.95 0.86 0.88 0.91 0.87 0.9 0.39 0.34 0.51 0.71 0.73 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0.07 0.1 0.07 0.24 0.14 0.44 0.21 0 0 0 0.06 0 0 0 0 0.11 0 0 0
Nucleus 0.38 0.46 0.16 0.14 0.16 0.1 0.09 0.15 0.1 0.14 0.11 0.1 0.05 0.12 0.12 0.05 0.08 0.05 0.33 0.41 0.17 0.12 0.08 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1 5 0 4 1
Bud 1 0 0 0 0 0 0 3 2 2 7 6 0 0 0 1 0 0 14 3 4 3 3 9
Bud Neck 1 1 12 6 12 7 7 9 7 7 9 14 0 1 1 1 1 4 2 0 2 0 0 2
Bud Site 0 0 0 0 2 0 1 0 0 2 2 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 1 2 0 2 1 2 0 1 2 2 1 2 1 4 12 5 0 0 5 0 1 1
Cytoplasm 217 92 247 201 314 317 311 303 199 266 201 273 153 269 370 310 379 343 154 73 138 147 175 281
Endoplasmic Reticulum 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 33 35 36 1 0 0 0 0 4
Endosome 1 0 0 0 1 1 0 0 1 2 0 0 0 3 0 2 2 3 5 1 4 2 4 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 2 4 1 0 0 2
Mitochondria 26 9 0 5 2 28 37 26 65 50 153 82 0 0 1 22 10 17 15 8 30 5 8 11
Nucleus 150 94 47 35 61 38 33 61 28 49 38 39 8 36 51 16 33 20 132 90 47 25 19 23
Nuclear Periphery 1 0 1 0 2 0 0 0 0 1 1 1 0 1 0 0 0 2 0 0 0 0 0 0
Nucleolus 47 23 1 8 2 4 7 6 6 10 15 15 1 2 1 2 11 3 27 19 7 7 5 23
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 1 2 2 3 4 8 9 7 11 6 13 0 0 0 2 3 2 23 3 3 3 4 13
Vac/Vac Membrane 2 4 3 1 0 0 5 3 1 9 3 3 1 0 2 5 7 5 7 9 6 5 4 16
Unique Cell Count 396 205 300 247 378 377 378 397 276 358 345 383 161 311 422 340 438 381 398 220 272 208 239 403
Labelled Cell Count 447 225 314 261 399 401 411 424 316 410 437 451 164 315 427 399 496 440 398 220 272 208 239 403


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 7.4 5.5 5.1 5.4 4.3 4.6 5.0 3.9 4.3 3.5 3.8 5.1 5.6 5.5 5.9 6.6 5.7 5.5 5.3 5.8
Std Deviation (1e-4) 2.5 3.3 1.9 1.9 2.1 1.5 2.0 1.8 1.4 1.7 1.4 1.5 1.7 1.9 1.9 2.2 2.9 2.2 2.1 1.7 2.2
Intensity Change (Log2) – – – -0.1 -0.01 -0.36 -0.23 -0.13 -0.49 -0.35 -0.65 -0.54 -0.09 0.04 0.02 0.12 0.27 0.05 0.01 -0.06 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck -1.0 -0.6 0 0 -1.3 0 0 0 0 0 -3.1 -3.7 -3.3 -3.8 -2.5
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0
Cytoplasm -0.3 0.3 0.6 0 -1.9 -2.9 -2.5 -6.6 -3.4 3.8 1.4 2.0 3.3 1.6 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 5.5 5.0 5.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 4.5 0 0 0 0 0 0 0 4.5 0 3.7
Nucleus -0.5 0.2 -2.2 -2.8 -0.1 -2.0 -0.7 -1.7 -2.1 -3.4 -1.5 -1.4 -4.6 -3.5 -4.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 1.4 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.307 42.6677 37.9935 33.4221 39.3145 37.0404 35.5281 30.3164 23.5138 32.0031
Translational Efficiency 1.1299 1.0172 0.9924 0.8188 0.8017 0.7073 0.8635 0.6888 0.8044 0.7097

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
320 1616 1145 1709 1466 1989 1186 1549 1786 3605 2331 3258

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 561.09 662.47 745.62 720.61 642.32 647.84 792.72 739.02 627.77 654.40 769.58 729.36
Standard Deviation 67.01 88.40 90.80 113.23 73.70 87.24 95.79 110.96 78.95 88.07 96.30 112.53
Intensity Change Log 2 — 0.239623 0.410208 0.360986 — 0.012345 0.303519 0.202321 — 0.122780 0.354246 0.278474

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000036 0.000605 0.000161 0.000420 0.000059 0.000329 0.000199 0.000695 0.000055 0.000452 0.000180 0.000550
Bud Neck 0.019940 0.045107 0.004237 0.013088 0.014953 0.047363 0.008027 0.014241 0.015846 0.046352 0.006165 0.013636
Bud Site 0.001537 0.015018 0.007869 0.046467 0.001441 0.025838 0.011689 0.036165 0.001458 0.020988 0.009813 0.041569
Cell Periphery 0.000304 0.000121 0.000112 0.000249 0.000141 0.000163 0.000134 0.000207 0.000170 0.000144 0.000123 0.000229
Cytoplasm 0.414526* 0.332872* 0.160672* 0.530104* 0.431460* 0.350207* 0.321611* 0.439771* 0.428426* 0.342437* 0.242557* 0.487155*
Cytoplasmic Foci 0.286122* 0.265952* 0.004708 0.017165 0.228108* 0.255901* 0.006921 0.013402 0.238502* 0.260407* 0.005834 0.015376
Eisosomes 0.000399 0.000146 0.000022 0.000037 0.000159 0.000148 0.000033 0.000036 0.000202 0.000147 0.000027 0.000037
Endoplasmic Reticulum 0.000777 0.000871 0.002236 0.000975 0.000588 0.000660 0.002119 0.001737 0.000622 0.000755 0.002177 0.001337
Endosome 0.005706 0.027835 0.000906 0.008459 0.011356 0.028270 0.002287 0.005643 0.010343 0.028075 0.001609 0.007120
Golgi 0.001727 0.007020 0.000045 0.004817 0.001799 0.006422 0.000147 0.003530 0.001786 0.006690 0.000097 0.004205
Lipid Particles 0.021102 0.009551 0.000775 0.000909 0.013249 0.009028 0.000924 0.000786 0.014656 0.009263 0.000851 0.000851
Mitochondria 0.007967 0.026804 0.000579 0.004015 0.004043 0.025432 0.001057 0.003868 0.004746 0.026047 0.000822 0.003945
Mitotic Spindle 0.000541 0.004955 0.001206 0.048786 0.001453 0.014996 0.002312 0.043058 0.001290 0.010495 0.001769 0.046063
None 0.003069 0.002203 0.004465 0.007487 0.004618 0.003618 0.006562 0.005119 0.004341 0.002983 0.005532 0.006361
Nuclear Periphery 0.000527 0.000705 0.001735 0.001344 0.000756 0.000717 0.001580 0.001823 0.000715 0.000712 0.001656 0.001572
Nuclear Periphery Foci 0.000990 0.000306 0.000315 0.000843 0.000282 0.000423 0.000608 0.001787 0.000409 0.000371 0.000464 0.001292
Nucleolus 0.002121 0.002256 0.000652 0.000961 0.001705 0.003626 0.000671 0.000685 0.001780 0.003012 0.000661 0.000830
Nucleus 0.205583* 0.140408* 0.795299* 0.232433* 0.236764* 0.129205 0.593913* 0.357234* 0.231177* 0.134227* 0.692835* 0.291769*
Peroxisomes 0.006456 0.026427 0.000120 0.001849 0.007422 0.024144 0.000354 0.001759 0.007249 0.025167 0.000239 0.001806
Vacuole 0.019736 0.087823 0.013750 0.076190 0.038682 0.069582 0.038448 0.066247 0.035287 0.077759 0.026317 0.071463
Vacuole Periphery 0.000833 0.003016 0.000134 0.003402 0.000964 0.003929 0.000403 0.002209 0.000940 0.003519 0.000271 0.002835

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.00 -8.51 -7.50 1.77 -2.69 -9.12 -5.87 -4.25 -1.54 -2.46 -6.06 -9.29 -6.76 0.45 -3.35
Bud Neck -7.30 5.88 3.29 13.34 -5.53 -12.79 4.59 1.61 14.39 -3.25 -15.32 7.26 2.99 19.79 -5.81
Bud Site -10.52 -3.06 -10.43 -4.73 -6.70 -11.50 -3.92 -8.76 0.94 -4.83 -15.03 -4.98 -13.70 -2.18 -8.22
Cell Periphery 5.83 5.57 3.93 -3.50 -2.95 -1.55 1.68 -0.88 0.32 -1.90 2.79 4.58 0.57 -1.80 -3.53
Cytoplasm 5.54 18.21 -1.56 -10.72 -30.87 8.76 15.25 6.44 -1.99 -9.00 11.32 27.27 2.92 -9.25 -26.97
Cytoplasmic Foci 1.69 23.87 23.03 43.64 -9.25 -3.56 40.13 39.07 46.50 -4.90 -3.25 46.38 44.81 63.68 -10.16
Eisosomes 5.00 7.36 7.02 17.99 -7.84 1.27 16.41 16.72 12.86 -0.17 4.77 15.33 14.58 20.10 -4.85
Endoplasmic Reticulum -0.45 -8.12 -3.33 -7.52 8.21 -0.84 -14.16 -12.76 -13.63 3.27 -1.64 -17.70 -13.21 -15.60 8.25
Endosome -13.15 6.99 1.07 13.59 -5.04 -10.13 9.56 7.69 16.94 -2.19 -13.71 11.17 7.15 21.41 -5.25
Golgi -6.25 4.58 -0.72 4.77 -3.62 -7.48 6.45 0.23 6.12 -2.90 -9.64 7.66 -0.43 7.55 -4.63
Lipid Particles 4.32 7.55 7.53 15.01 -0.51 3.98 13.35 13.60 13.13 1.82 5.68 15.37 15.46 19.50 0.89
Mitochondria -6.17 3.36 2.47 11.79 -4.79 -12.13 5.43 2.13 13.02 -2.45 -14.89 6.61 3.38 17.63 -4.71
Mitotic Spindle -4.39 -1.48 -9.95 -8.27 -9.62 -6.80 -1.21 -7.83 -3.21 -7.40 -7.32 -1.14 -12.23 -7.60 -12.04
None 1.17 -1.02 -3.55 -7.25 -3.35 1.10 -0.70 0.14 -1.67 1.26 1.86 -0.42 -1.39 -5.94 -1.48
Nuclear Periphery -2.06 -15.04 -16.40 -13.93 -2.10 0.25 -15.78 -7.31 -7.23 -2.12 -0.21 -19.85 -12.80 -12.44 -2.83
Nuclear Periphery Foci 1.43 1.23 0.75 -1.99 -1.37 -1.40 -7.01 -2.21 -1.89 -0.87 0.57 -1.90 -2.07 -2.50 -1.30
Nucleolus -0.04 2.23 2.00 5.70 -1.77 -3.77 3.59 5.65 6.54 1.03 -3.50 4.84 5.27 8.24 0.08
Nucleus 5.80 -48.57 -9.40 -21.86 49.66 15.87 -36.56 -19.65 -34.91 16.83 16.75 -61.17 -19.10 -39.76 43.54
Peroxisomes -10.30 7.01 5.76 14.83 -2.71 -10.10 8.24 6.79 15.91 -1.70 -13.62 9.67 8.17 21.79 -3.03
Vacuole -18.10 -0.88 -22.03 -7.74 -22.06 -10.02 -8.19 -15.91 -8.97 -6.94 -17.18 -4.98 -24.92 -12.17 -19.14
Vacuole Periphery -8.31 4.90 -2.16 2.68 -4.01 -6.88 4.73 -0.59 5.60 -2.96 -9.58 6.82 -1.96 5.81 -4.94
Standard name
Human Ortholog
Description RNA polymerase III subunit C82
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Rpc82

Rpc82


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpc82-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available