Standard name
Human Ortholog
Description Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.07 0 0 0 – – 0.1 0 0 – – – 0 0 0 0 0 0 0.24 0 0
Bud Neck 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 0.05 0
Bud Site 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – – 0.05 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.85 0.95 1.0 0.96 0.89 0.91 – – 0.95 0.89 0.82 – – – 0.63 0.77 0.75 0.27 0.33 0.33 0.45 0.45 0.2
Endoplasmic Reticulum 0.1 0.19 0 0 0.08 0.08 0.05 – – 0 0 0.06 – – – 0 0 0 0.33 0.34 0.11 0 0 0.11
Endosome 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – – 0 0.11 0.09 – – – 0 0 0 0 0 0.07 0 0 0.08
Nucleus 0 0 0 0 0 0 0 – – 0 0 0.12 – – – 0.05 0 0 0 0 0 0 0 0.15
Nuclear Periphery 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 0.08 0
Vac/Vac Membrane 0 0 0.05 0 0 0 0 – – 0 0 0 – – – 0.39 0.25 0.28 0.28 0.19 0.4 0.06 0.18 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 1 4 – – 0 1 0 – – – 1 0 0 2 2 0 0 0 0
Bud 2 0 0 2 0 0 2 – – 2 3 1 – – – 0 0 0 1 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 2 4 1 0 0 0
Bud Site 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 1 0 1 – – 1 1 1 – – – 1 3 2 1 0 0 0 0 0
Cytoplasm 154 86 18 27 25 55 86 – – 19 85 27 – – – 130 172 122 53 38 14 1 5 1
Endoplasmic Reticulum 18 19 0 0 2 5 5 – – 0 1 2 – – – 8 1 1 65 39 4 0 0 0
Endosome 0 0 0 0 0 0 0 – – 0 2 0 – – – 7 12 6 4 1 0 0 0 0
Golgi 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 1 2 0 0 0 0 0
Mitochondria 3 0 0 0 0 2 0 – – 0 10 3 – – – 0 2 0 6 2 2 0 0 0
Nucleus 0 0 0 0 0 0 1 – – 0 1 4 – – – 11 5 4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – – 0 0 1 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – – 0 0 0 – – – 0 0 0 0 0 0 0 1 0
Vac/Vac Membrane 2 0 1 0 0 0 0 – – 0 2 0 – – – 81 55 46 55 21 17 0 2 0
Unique Cell Count 174 101 19 27 26 62 94 20 95 33 206 222 163 199 116 45 4 12 5
Labelled Cell Count 180 106 19 29 28 63 99 22 106 39 239 250 182 199 116 45 4 12 5


Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 7.0 7.4 6.6 7.5 6.1 6.3 – – 5.4 4.8 4.9 – – – 8.9 9.3 8.6 – – –
Std Deviation (1e-4) 0.7 1.2 2.3 0.7 0.8 1.0 1.3 – – 0.5 0.9 1.4 – – – 1.5 1.6 1.5 – – –
Intensity Change (Log2) – – – -0.18 0.01 -0.28 -0.23 – – -0.47 -0.63 -0.61 – – – 0.26 0.32 0.21 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – – 0 0 0 – – – 0 0 0
Bud 0 0 0 0 – – 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 0 – – 0 0 0 – – – 0 0 0
Bud Site 0 0 0 0 – – 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 0 – – 0 0 0 – – – 0 0 0
Cytoplasm 1.2 0.2 -0.8 -0.5 – – 0 -0.7 -1.3 – – – -2.8 -1.8 -1.9
Endoplasmic Reticulum 0 0 0 0 – – 0 0 0 – – – 0 0 0
Endosome 0 0 0 0 – – 0 0 0 – – – 0 0 0
Golgi 0 0 0 0 – – 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 0 – – 0 0 0 – – – 0 0 0
Nucleus 0 0 0 0 – – 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 0 – – 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 0 – – 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 0 – – 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 0 – – 0 0 0 – – – 0 0 0
Vacuole 0 0 0 0 – – 0 0 0 – – – 3.0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6247 2.0165 1.6075 1.6942 1.3942 1.8246 1.4043 2.0542 1.8228 1.2987 1.7551 2.0478 1.0935 1.6405 1.3591 1.6848 1.3012 1.5435
Actin 0.0218 0.0054 0.0071 0.0079 0.0089 0.0107 0.0135 0.0016 0.0046 0.0027 0.0268 0.0159 0.0281 0.0006 0.0176 0.0066 0.1138 0.0147
Bud 0.0012 0.0128 0.0024 0.0018 0.0008 0.0017 0.0006 0.0003 0.0004 0.0003 0.0014 0.0007 0.0003 0.0015 0.0019 0.0005 0.0009 0.0112
Bud Neck 0.0065 0.002 0.0048 0.0034 0.0028 0.0066 0.0014 0.0008 0.0033 0.0058 0.002 0.0034 0.0012 0.0003 0.0014 0.0004 0.0009 0.0016
Bud Periphery 0.0027 0.0028 0.0037 0.0017 0.0013 0.0017 0.0015 0.0008 0.0009 0.0012 0.0032 0.0028 0.0003 0.0006 0.0036 0.0008 0.0012 0.0039
Bud Site 0.008 0.0311 0.0129 0.0009 0.0021 0.0009 0.0067 0.0119 0.0131 0.001 0.0033 0.001 0.0032 0.0243 0.0048 0.0008 0.0034 0.0007
Cell Periphery 0.0045 0.0036 0.0033 0.0012 0.0022 0.0011 0.0051 0.0095 0.0058 0.0085 0.0019 0.0021 0.0023 0.003 0.0022 0.0004 0.0011 0.0009
Cytoplasm 0.4717 0.5863 0.5276 0.6091 0.6218 0.5891 0.4546 0.5685 0.4875 0.602 0.5215 0.5575 0.489 0.616 0.5988 0.4394 0.5345 0.6661
Cytoplasmic Foci 0.0264 0.0213 0.0306 0.0293 0.0407 0.0456 0.0447 0.0225 0.0287 0.0185 0.0255 0.0228 0.033 0.0124 0.0201 0.0629 0.0096 0.0335
Eisosomes 0.0011 0.0006 0.0008 0.0002 0.0001 0.0001 0.0005 0.0002 0.0003 0.0002 0.0002 0.0002 0.0003 0.0001 0.0002 0.0001 0.0005 0.0001
Endoplasmic Reticulum 0.1462 0.12 0.1092 0.0566 0.0895 0.0982 0.1513 0.1524 0.145 0.1512 0.1158 0.1074 0.2049 0.1561 0.1517 0.1984 0.233 0.1365
Endosome 0.0544 0.0392 0.0501 0.0521 0.074 0.039 0.0568 0.0362 0.0357 0.0613 0.0804 0.0272 0.0226 0.0214 0.0186 0.0437 0.0359 0.0194
Golgi 0.0082 0.0023 0.0115 0.0095 0.0069 0.0067 0.0136 0.0023 0.0092 0.0093 0.0133 0.008 0.0067 0.0011 0.0087 0.021 0.0212 0.0119
Lipid Particles 0.0213 0.0121 0.0187 0.0166 0.0124 0.0173 0.0291 0.0235 0.0165 0.0072 0.0368 0.0087 0.0203 0.0056 0.0055 0.0504 0.0064 0.0172
Mitochondria 0.0132 0.0009 0.0089 0.0373 0.004 0.006 0.0116 0.0009 0.0094 0.014 0.0109 0.0131 0.0029 0.0026 0.0061 0.0519 0.0085 0.0036
None 0.1626 0.1217 0.1543 0.1168 0.0618 0.1455 0.1662 0.1364 0.2055 0.1025 0.0677 0.2044 0.1532 0.1336 0.1458 0.0842 0.0138 0.0585
Nuclear Periphery 0.0125 0.0077 0.0074 0.0053 0.0071 0.0032 0.0056 0.0046 0.0038 0.0024 0.0039 0.0059 0.0039 0.0035 0.003 0.0049 0.0051 0.0019
Nucleolus 0.0014 0.0003 0.001 0.0016 0.0019 0.0005 0.0007 0.0002 0.0013 0.0001 0.0011 0.0005 0.0003 0.0009 0.0001 0.0002 0.0001 0.0034
Nucleus 0.0065 0.0037 0.0062 0.0055 0.011 0.0036 0.0022 0.0025 0.0028 0.0015 0.0032 0.0019 0.0014 0.0023 0.001 0.0011 0.0011 0.0022
Peroxisomes 0.0051 0.0029 0.0148 0.005 0.0178 0.007 0.0111 0.0025 0.0091 0.0023 0.0236 0.0034 0.0149 0.0001 0.0015 0.0183 0.0015 0.0027
Punctate Nuclear 0.0063 0.0012 0.0072 0.0144 0.0034 0.0054 0.0035 0.001 0.0016 0.0006 0.0186 0.0031 0.0044 0.0008 0.0006 0.005 0.0009 0.0008
Vacuole 0.0169 0.0208 0.0159 0.0213 0.0258 0.0092 0.0179 0.0207 0.0144 0.0067 0.0355 0.0088 0.0063 0.0129 0.0062 0.0081 0.0055 0.0081
Vacuole Periphery 0.0017 0.0012 0.0015 0.0025 0.0037 0.0008 0.0017 0.0005 0.001 0.0007 0.0034 0.001 0.0004 0.0005 0.0005 0.0012 0.0011 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.6569 39.6533 34.3285 41.4292 33.5487 28.9399 44.6875 44.9127 40.8064 50.8998
Translational Efficiency 0.5987 0.5822 0.6428 0.4037 0.4726 0.8397 0.5576 0.4319 0.4676 0.4159

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
262 1332 200 1315 2355 1471 148 304 2617 2803 348 1619

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 759.19 780.53 1059.10 929.41 801.63 798.57 1058.59 1008.50 797.38 790.00 1058.88 944.26
Standard Deviation 121.14 128.16 115.18 128.97 93.89 110.28 113.69 125.21 97.79 119.45 114.55 131.94
Intensity Change Log 2 — 0.039993 0.480306 0.291854 — -0.005518 0.401136 0.331203 — 0.016798 0.440187 0.312197

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000338 0.000712 0.003182 0.008832 0.000244 0.001168 0.001417 0.005507 0.000253 0.000951 0.002431 0.008208
Bud Neck 0.030643 0.013491 0.010222 0.014816 0.011012 0.017863 0.004835 0.018729 0.012977 0.015786 0.007931 0.015550
Bud Site 0.016960 0.009063 0.022442 0.062836 0.003551 0.019203 0.009039 0.054546 0.004893 0.014384 0.016742 0.061280
Cell Periphery 0.000426 0.000329 0.001404 0.004030 0.000390 0.000405 0.001128 0.001581 0.000394 0.000369 0.001287 0.003570
Cytoplasm 0.494889* 0.371027* 0.257033* 0.344978* 0.459881* 0.348295* 0.263139* 0.270426* 0.463386* 0.359097* 0.259630* 0.330980*
Cytoplasmic Foci 0.178606 0.229714* 0.044769 0.056715 0.144735 0.189826* 0.049469 0.073546 0.148126 0.208781* 0.046768 0.059875
Eisosomes 0.000367 0.000341 0.000218 0.001518 0.000572 0.000777 0.000336 0.000730 0.000551 0.000570 0.000268 0.001370
Endoplasmic Reticulum 0.005259 0.019324 0.069432 0.102699* 0.017404 0.038631 0.047531 0.084638 0.016188 0.029456 0.060118 0.099308
Endosome 0.014302 0.037753 0.020400 0.009845 0.017900 0.042264 0.018613 0.015352 0.017540 0.040121 0.019640 0.010879
Golgi 0.001424 0.006290 0.000694 0.003416 0.002792 0.011162 0.000234 0.002421 0.002655 0.008847 0.000498 0.003230
Lipid Particles 0.008863 0.029223 0.017493 0.018406 0.022361 0.044215 0.020036 0.023644 0.021009 0.037091 0.018575 0.019389
Mitochondria 0.001004 0.002693 0.000494 0.002366 0.001072 0.007072 0.000217 0.001363 0.001065 0.004991 0.000377 0.002178
Mitotic Spindle 0.002744 0.001672 0.002327 0.006610 0.001093 0.004511 0.000817 0.009391 0.001258 0.003162 0.001685 0.007132
None 0.001195 0.002779 0.000624 0.001219 0.001112 0.001053 0.001228 0.004067 0.001120 0.001873 0.000881 0.001754
Nuclear Periphery 0.000736 0.001062 0.005642 0.000923 0.001417 0.001854 0.004202 0.001509 0.001349 0.001478 0.005030 0.001033
Nuclear Periphery Foci 0.001695 0.003673 0.052343 0.015133 0.001532 0.001669 0.052158 0.024099 0.001548 0.002621 0.052265 0.016817
Nucleolus 0.001506 0.001544 0.000336 0.000301 0.000662 0.000913 0.000280 0.000380 0.000746 0.001213 0.000313 0.000315
Nucleus 0.112312 0.066056 0.204917* 0.108865 0.137253* 0.064626 0.106896 0.055976 0.134756* 0.065306 0.163230* 0.098934
Peroxisomes 0.003969 0.005311 0.000816 0.003292 0.002420 0.006708 0.000631 0.003196 0.002575 0.006044 0.000737 0.003274
Vacuole 0.121702 0.195586 0.284630* 0.231490* 0.171836 0.193933 0.417597* 0.345905* 0.166817 0.194719 0.341179* 0.252974*
Vacuole Periphery 0.001061 0.002356 0.000581 0.001709 0.000762 0.003851 0.000196 0.002992 0.000792 0.003140 0.000417 0.001950

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.22 -5.01 -8.61 -8.14 -4.45 -4.76 -4.66 -4.05 -2.97 -2.84 -6.06 -6.22 -9.44 -8.35 -5.87
Bud Neck 2.20 2.56 2.15 -0.28 -1.93 -5.16 2.73 -1.27 0.81 -2.50 -2.51 2.87 -1.00 1.37 -3.76
Bud Site 1.75 -0.99 -6.97 -11.42 -5.32 -7.31 -3.67 -5.78 -3.02 -4.45 -7.38 -4.32 -13.72 -10.40 -7.84
Cell Periphery 1.23 -5.31 -7.99 -8.31 -4.89 -0.48 -4.43 -4.62 -4.50 -1.04 0.85 -7.02 -8.55 -8.63 -5.50
Cytoplasm 5.71 10.58 8.23 4.90 -5.34 14.35 11.62 14.30 6.81 -0.69 15.82 18.18 19.89 6.65 -5.67
Cytoplasmic Foci -4.89 12.04 11.49 32.30 -2.53 -8.74 20.75 13.72 18.76 -2.47 -13.94 27.55 27.37 40.56 -3.71
Eisosomes 0.09 1.05 -4.10 -5.18 -6.00 -2.20 5.40 0.76 2.25 -2.16 -0.33 7.28 -3.70 -3.48 -6.11
Endoplasmic Reticulum -4.83 -8.76 -13.19 -10.91 -1.63 -6.82 -2.89 -3.82 -1.20 -1.32 -6.37 -7.64 -12.44 -9.07 -3.18
Endosome -9.97 -1.86 2.88 17.03 5.55 -13.98 1.77 5.28 15.86 2.40 -17.57 0.35 9.43 23.93 6.35
Golgi -6.48 1.75 -1.66 4.80 -3.61 -8.37 10.52 1.76 7.83 -1.39 -9.74 9.20 0.93 9.10 -4.16
Lipid Particles -10.01 -3.89 -4.90 7.01 -0.08 -9.86 3.41 2.29 9.14 -0.57 -10.42 3.77 4.05 13.23 -0.41
Mitochondria -3.34 1.68 -1.18 1.26 -2.07 -7.35 11.88 0.68 6.97 -1.55 -8.16 8.75 -1.02 4.97 -2.40
Mitotic Spindle 0.65 0.07 -1.77 -2.76 -1.59 -2.76 -0.06 -2.48 -1.40 -2.46 -2.29 -0.62 -3.96 -2.44 -2.87
None -1.64 2.28 0.28 1.80 -2.89 0.08 0.41 -1.12 -1.11 -1.20 -1.61 1.84 -0.96 0.85 -2.12
Nuclear Periphery -1.34 -5.95 -1.66 0.17 5.71 -1.61 -4.61 -0.23 1.24 4.37 -0.65 -6.72 1.48 2.38 7.38
Nuclear Periphery Foci -4.03 -8.87 -13.18 -10.90 6.40 -0.96 -7.12 -8.99 -8.91 3.65 -5.80 -11.43 -17.03 -15.19 7.63
Nucleolus 0.03 1.81 1.85 5.75 0.56 -2.47 8.21 3.21 4.21 -0.89 -3.47 5.37 5.07 7.47 -0.12
Nucleus 4.88 -5.14 0.32 -9.84 6.42 20.42 2.26 12.43 0.28 4.09 21.70 -2.97 7.66 -10.37 6.14
Peroxisomes -2.02 4.55 1.42 4.08 -3.03 -6.47 10.76 0.52 5.64 -3.23 -8.57 10.98 0.12 5.83 -3.72
Vacuole -4.21 -8.84 -11.38 -11.55 1.31 -3.61 -13.93 -15.64 -14.18 0.93 -5.38 -15.37 -20.66 -17.08 4.02
Vacuole Periphery -3.69 1.67 -0.39 2.01 -1.39 -5.60 15.90 -0.61 1.55 -1.05 -7.43 4.93 -1.05 3.24 -1.91
Standard name
Human Ortholog
Description Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum
Localization
Cell Percentages ER (19%), vacuole (14%), mixed (49%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Yps7

Yps7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yps7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available