Standard name | |
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Human Ortholog | |
Description | Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; overexpression of human AFG3L2 complements respiratory defect of yeast afg3 yta12 double null mutation, but overexpression of disease-associated AFG3L2 variants does not; expression of both human SPG7 (paraplegin) and AFG3L2 complements yeast yta12 afg3 double mutation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 2 | 1 | 2 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 1 | 0 | 5 | 2 | 0 | 1 | 9 | 11 | 10 | 8 | 11 | 15 |
Bud | 3 | 5 | 4 | 5 | 2 | 4 | 7 | 19 | 12 | 10 | 10 | 7 | 1 | 1 | 1 | 9 | 5 | 4 | 1 | 1 | 0 | 2 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 2 | 4 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 11 | 12 | 13 | 5 | 3 | 0 | 1 | 3 | 4 | 4 | 6 | 2 | 3 | 3 | 8 | 26 | 17 | 13 | 0 | 2 | 1 | 0 | 0 | 0 |
Cytoplasm | 1 | 4 | 7 | 6 | 12 | 7 | 8 | 16 | 3 | 7 | 6 | 5 | 3 | 3 | 6 | 37 | 14 | 17 | 0 | 1 | 0 | 0 | 2 | 0 |
Endoplasmic Reticulum | 1 | 4 | 2 | 2 | 4 | 5 | 2 | 4 | 3 | 3 | 9 | 23 | 1 | 1 | 6 | 7 | 8 | 10 | 1 | 1 | 3 | 0 | 2 | 1 |
Endosome | 5 | 0 | 9 | 4 | 12 | 3 | 5 | 7 | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 6 | 2 | 3 | 7 | 5 | 8 | 4 | 9 | 7 |
Golgi | 43 | 13 | 16 | 6 | 8 | 0 | 1 | 2 | 1 | 2 | 2 | 0 | 41 | 39 | 135 | 57 | 38 | 49 | 38 | 29 | 21 | 15 | 25 | 40 |
Mitochondria | 485 | 279 | 362 | 251 | 274 | 406 | 616 | 599 | 528 | 467 | 487 | 477 | 93 | 93 | 124 | 14 | 8 | 6 | 501 | 246 | 338 | 162 | 228 | 289 |
Nucleus | 2 | 1 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 3 | 0 | 1 | 1 | 1 | 4 | 2 | 1 | 2 | 1 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 23 | 14 | 33 | 17 | 33 | 38 | 88 | 63 | 75 | 62 | 49 | 62 | 4 | 4 | 5 | 20 | 5 | 5 | 4 | 4 | 3 | 0 | 2 | 2 |
Nucleolus | 24 | 12 | 16 | 13 | 26 | 67 | 76 | 80 | 66 | 54 | 40 | 31 | 0 | 0 | 1 | 7 | 6 | 8 | 2 | 4 | 0 | 0 | 1 | 5 |
Peroxisomes | 6 | 1 | 29 | 8 | 11 | 3 | 15 | 12 | 5 | 1 | 0 | 5 | 2 | 2 | 4 | 14 | 7 | 8 | 5 | 3 | 40 | 19 | 27 | 35 |
SpindlePole | 0 | 0 | 2 | 0 | 2 | 2 | 2 | 6 | 3 | 3 | 2 | 3 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 96 | 31 | 27 | 15 | 34 | 25 | 20 | 23 | 17 | 19 | 13 | 12 | 32 | 34 | 53 | 37 | 20 | 19 | 12 | 13 | 3 | 4 | 8 | 12 |
Unique Cell Count | 548 | 311 | 413 | 270 | 338 | 455 | 691 | 701 | 566 | 501 | 516 | 523 | 120 | 121 | 226 | 212 | 116 | 122 | 590 | 328 | 434 | 225 | 329 | 418 |
Labelled Cell Count | 701 | 378 | 521 | 334 | 422 | 562 | 846 | 839 | 722 | 641 | 628 | 633 | 184 | 183 | 354 | 240 | 132 | 146 | 590 | 328 | 434 | 225 | 329 | 418 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.5 | 13.1 | 10.3 | 10.5 | 10.8 | 9.2 | 9.3 | 9.4 | 9.1 | 8.5 | 8.8 | 9.0 | 12.6 | 12.6 | 12.2 | 8.9 | 9.7 | 11.6 | 12.6 | 13.4 | 14.5 |
Std Deviation (1e-4) | 2.5 | 2.6 | 2.1 | 2.5 | 2.6 | 2.4 | 2.4 | 2.5 | 2.5 | 2.5 | 2.5 | 3.2 | 2.3 | 2.3 | 1.9 | 1.8 | 2.2 | 2.8 | 2.7 | 2.9 | 3.0 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.07 | -0.16 | -0.14 | -0.12 | -0.17 | -0.28 | -0.23 | -0.2 | 0.3 | 0.3 | 0.24 | -0.2 | -0.08 | 0.18 | 0.29 | 0.38 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2.0 | 1.4 | 1.3 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.0 | -2.1 | -3.8 | -4.3 | -3.7 | -2.9 | -2.6 | -2.1 | -3.3 | 0 | 0 | 0.3 | 4.5 | 4.7 | 3.4 |
Cytoplasm | 0.5 | 1.6 | -0.2 | -0.7 | 0.7 | 0 | -0.4 | -0.7 | -1.0 | 0 | 0 | 0 | 7.3 | 0 | 5.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.7 | 1.1 | -1.9 | -2.1 | -1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0 | 0 |
Golgi | -1.2 | -1.2 | -4.2 | -4.9 | -4.6 | -4.4 | -3.8 | -3.8 | -4.5 | 9.5 | 9.0 | 15.9 | 8.5 | 9.1 | 10.8 |
Mitochondria | 2.2 | -2.5 | 0.7 | 0.8 | -1.0 | 3.0 | 2.9 | 3.6 | 1.8 | -2.8 | -2.9 | -9.3 | -19.6 | -16.8 | -17.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.8 | 0.9 | 0.2 | 2.4 | 0.6 | 2.6 | 2.2 | 0.8 | 1.9 | -1.8 | -1.8 | -3.0 | 0.6 | -1.4 | -1.5 |
Nucleolus | 0.6 | 2.3 | 5.4 | 4.1 | 4.3 | 4.3 | 3.9 | 2.5 | 1.4 | 0 | 0 | -2.6 | -0.4 | 0 | 1.3 |
Peroxisomes | -2.3 | -2.3 | -5.0 | -4.0 | -4.5 | -5.2 | -5.8 | -6.1 | -4.9 | -2.2 | -2.2 | -2.9 | -0.2 | -0.4 | -0.2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.5 | 1.8 | -0.6 | -2.9 | -2.5 | -2.6 | -1.9 | -3.0 | -3.2 | 6.2 | 6.6 | 6.2 | 4.3 | 3.6 | 3.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.3596 | 11.647 | 10.636 | 9.6717 | 10.0265 | 10.3415 | 12.0329 | 13.2142 | 12.6553 | 10.5194 | 11.7284 | 12.7137 | 13.0923 | 13.0242 | 13.1891 | 12.8436 | 12.9268 | 13.0501 |
Actin | 0.0094 | 0.0029 | 0.009 | 0.0013 | 0.0008 | 0.0024 | 0.0223 | 0.0097 | 0.0127 | 0.0009 | 0.0038 | 0.0074 | 0.0085 | 0.008 | 0.0037 | 0.0028 | 0.0003 | 0.0006 |
Bud | 0.0005 | 0.0006 | 0.0015 | 0.0002 | 0 | 0 | 0.0017 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0 |
Bud Neck | 0.0011 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Bud Periphery | 0.0012 | 0.0011 | 0.0019 | 0.0008 | 0.0001 | 0.0001 | 0.0029 | 0.0004 | 0.0006 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Bud Site | 0.001 | 0.0022 | 0.0031 | 0.0001 | 0.0001 | 0 | 0.0017 | 0.0073 | 0.0012 | 0.0001 | 0.0006 | 0 | 0.0008 | 0.0009 | 0 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0006 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0009 | 0 | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0011 | 0.0002 | 0.0006 | 0.0003 | 0.015 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0076 | 0.0012 | 0.0016 | 0.0003 | 0.0008 | 0.0017 | 0.0029 | 0.0038 | 0.003 | 0.0052 | 0.027 | 0.0005 | 0.0009 | 0.0034 | 0.0007 | 0.0005 | 0.0009 | 0.0002 |
Eisosomes | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0007 | 0.0002 | 0.0002 | 0 | 0.0004 | 0.0012 | 0.0008 | 0.0001 | 0.0012 | 0.0007 | 0.0017 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 |
Endosome | 0.0082 | 0.0065 | 0.0036 | 0.0074 | 0.007 | 0.0051 | 0.008 | 0.0117 | 0.009 | 0.0175 | 0.0254 | 0.0023 | 0.003 | 0.0086 | 0.0016 | 0.002 | 0.0023 | 0.0021 |
Golgi | 0.0149 | 0.0204 | 0.0062 | 0.0183 | 0.0241 | 0.0185 | 0.0139 | 0.0254 | 0.0226 | 0.0092 | 0.0215 | 0.0131 | 0.0085 | 0.0245 | 0.0105 | 0.0096 | 0.0518 | 0.0058 |
Lipid Particles | 0.0297 | 0.0122 | 0.0037 | 0.0004 | 0.0079 | 0.0026 | 0.0052 | 0.0125 | 0.0042 | 0.0129 | 0.0159 | 0.0055 | 0.0018 | 0.0143 | 0.0025 | 0.0005 | 0.0004 | 0.0062 |
Mitochondria | 0.8781 | 0.9231 | 0.9524 | 0.9664 | 0.8756 | 0.9599 | 0.9162 | 0.9103 | 0.9335 | 0.9266 | 0.8545 | 0.9597 | 0.9682 | 0.9142 | 0.9762 | 0.9821 | 0.9286 | 0.975 |
None | 0.0007 | 0 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0006 | 0.0002 | 0.0017 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0009 | 0.0004 | 0.0003 | 0 | 0.0203 | 0.0029 | 0.0031 | 0.0008 | 0.0005 | 0.0013 | 0.0061 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0006 |
Nucleolus | 0.0115 | 0.0006 | 0.0001 | 0 | 0.0045 | 0 | 0.0007 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0 |
Nucleus | 0.0029 | 0.0002 | 0.0002 | 0 | 0.0195 | 0.0002 | 0.0041 | 0.0001 | 0.0002 | 0.0002 | 0.0103 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 |
Peroxisomes | 0.0026 | 0.0045 | 0.0086 | 0.0014 | 0.0018 | 0.0014 | 0.0058 | 0.0066 | 0.0049 | 0.0034 | 0.0066 | 0.0058 | 0.0063 | 0.0106 | 0.0033 | 0.0011 | 0.0146 | 0.001 |
Punctate Nuclear | 0.002 | 0 | 0.0003 | 0 | 0.0054 | 0.0001 | 0.0026 | 0 | 0.0004 | 0.0175 | 0.0025 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0105 | 0.0056 | 0.0016 | 0.0004 | 0.008 | 0.0004 | 0.0027 | 0.0022 | 0.0011 | 0.0014 | 0.0021 | 0.0007 | 0.0002 | 0.0051 | 0.0002 | 0.0002 | 0.0002 | 0.001 |
Vacuole Periphery | 0.0144 | 0.0174 | 0.0042 | 0.0025 | 0.0227 | 0.0028 | 0.0023 | 0.008 | 0.0026 | 0.002 | 0.0031 | 0.0042 | 0.001 | 0.0084 | 0.0009 | 0.0006 | 0.0006 | 0.0067 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.8601 | 40.9995 | 46.9643 | 47.1853 | 41.5674 | 60.5246 | 52.3213 | 55.958 | 63.6657 | 57.1947 |
Translational Efficiency | 0.8454 | 0.6256 | 0.605 | 0.692 | 0.5711 | 0.5796 | 0.555 | 0.555 | 0.4614 | 0.4931 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
407 | 1322 | 1212 | 1464 | 1916 | 1673 | 203 | 1903 | 2323 | 2995 | 1415 | 3367 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1182.44 | 994.25 | 1361.03 | 1165.60 | 1035.40 | 1117.17 | 1369.24 | 1205.88 | 1061.16 | 1062.91 | 1362.21 | 1188.37 |
Standard Deviation | 180.56 | 200.75 | 232.87 | 196.38 | 189.27 | 192.63 | 190.87 | 176.34 | 195.91 | 205.53 | 227.34 | 186.39 |
Intensity Change Log 2 | — | -0.250086 | 0.202932 | -0.020694 | — | 0.109661 | 0.403187 | 0.219898 | — | -0.070942 | 0.299888 | 0.096632 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.005813 | 0.004398 | 0.002480 | 0.004392 | 0.000874 | 0.001656 | 0.004715 | 0.001556 | 0.001740 | 0.002866 | 0.002801 | 0.002789 |
Bud Neck | 0.001161 | 0.001453 | 0.002812 | 0.001706 | 0.000280 | 0.000758 | 0.007154 | 0.000638 | 0.000435 | 0.001065 | 0.003435 | 0.001103 |
Bud Site | 0.007314 | 0.003135 | 0.002849 | 0.008890 | 0.000617 | 0.001570 | 0.006889 | 0.002533 | 0.001790 | 0.002261 | 0.003429 | 0.005297 |
Cell Periphery | 0.000998 | 0.000294 | 0.000157 | 0.001416 | 0.000086 | 0.000089 | 0.000163 | 0.000424 | 0.000246 | 0.000179 | 0.000158 | 0.000855 |
Cytoplasm | 0.001015 | 0.000405 | 0.000508 | 0.000811 | 0.000282 | 0.000853 | 0.000742 | 0.000437 | 0.000410 | 0.000656 | 0.000541 | 0.000599 |
Cytoplasmic Foci | 0.032577 | 0.012983 | 0.035188 | 0.006867 | 0.007048 | 0.011326 | 0.041559 | 0.006260 | 0.011521 | 0.012058 | 0.036102 | 0.006524 |
Eisosomes | 0.000178 | 0.000196 | 0.000101 | 0.000154 | 0.000117 | 0.000095 | 0.000115 | 0.000063 | 0.000127 | 0.000140 | 0.000103 | 0.000102 |
Endoplasmic Reticulum | 0.003332 | 0.000276 | 0.000336 | 0.000662 | 0.000112 | 0.000114 | 0.000410 | 0.000228 | 0.000676 | 0.000185 | 0.000347 | 0.000416 |
Endosome | 0.053871 | 0.010285 | 0.016960 | 0.010186 | 0.003637 | 0.007243 | 0.026141 | 0.009903 | 0.012438 | 0.008586 | 0.018278 | 0.010026 |
Golgi | 0.222390* | 0.026693 | 0.068929 | 0.034734 | 0.012715 | 0.023369 | 0.040877 | 0.034925 | 0.049451 | 0.024837 | 0.064904 | 0.034842 |
Lipid Particles | 0.000957 | 0.004733 | 0.005575 | 0.000619 | 0.002903 | 0.003168 | 0.002401 | 0.000277 | 0.002562 | 0.003859 | 0.005119 | 0.000426 |
Mitochondria | 0.546538* | 0.906379* | 0.762538* | 0.891838* | 0.961064* | 0.932779* | 0.785372* | 0.920751* | 0.888437* | 0.921126* | 0.765814* | 0.908179* |
Mitotic Spindle | 0.009062 | 0.000903 | 0.010612 | 0.004533 | 0.000267 | 0.000991 | 0.019368 | 0.002689 | 0.001808 | 0.000952 | 0.011868 | 0.003491 |
None | 0.000704 | 0.000712 | 0.001749 | 0.000665 | 0.000704 | 0.000776 | 0.000733 | 0.000402 | 0.000704 | 0.000748 | 0.001603 | 0.000516 |
Nuclear Periphery | 0.001120 | 0.000078 | 0.004244 | 0.000161 | 0.000126 | 0.000078 | 0.001644 | 0.000097 | 0.000300 | 0.000078 | 0.003871 | 0.000125 |
Nuclear Periphery Foci | 0.001325 | 0.000233 | 0.002304 | 0.000138 | 0.000091 | 0.000119 | 0.001048 | 0.000088 | 0.000307 | 0.000169 | 0.002124 | 0.000110 |
Nucleolus | 0.000075 | 0.001836 | 0.001133 | 0.000145 | 0.000053 | 0.000227 | 0.002561 | 0.000076 | 0.000057 | 0.000938 | 0.001338 | 0.000106 |
Nucleus | 0.000089 | 0.000499 | 0.000232 | 0.000107 | 0.000024 | 0.000777 | 0.000506 | 0.000044 | 0.000035 | 0.000654 | 0.000272 | 0.000071 |
Peroxisomes | 0.003814 | 0.019540 | 0.006895 | 0.005338 | 0.005397 | 0.007021 | 0.026413 | 0.004960 | 0.005120 | 0.012547 | 0.009695 | 0.005124 |
Vacuole | 0.044316 | 0.002386 | 0.024760 | 0.011068 | 0.001035 | 0.003428 | 0.016725 | 0.005876 | 0.008618 | 0.002968 | 0.023608 | 0.008133 |
Vacuole Periphery | 0.063352 | 0.002583 | 0.049638 | 0.015570 | 0.002568 | 0.003561 | 0.014462 | 0.007774 | 0.013217 | 0.003129 | 0.044592 | 0.011164 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.78 | 1.88 | 0.78 | 0.00 | -2.20 | -2.75 | -2.66 | -2.84 | 0.32 | 2.17 | -2.46 | -1.77 | -2.34 | 0.17 | 0.02 |
Bud Neck | -0.77 | -4.84 | -1.59 | -0.55 | 2.58 | -2.50 | -4.57 | -2.42 | 0.59 | 4.33 | -3.23 | -8.61 | -3.99 | -0.15 | 6.29 |
Bud Site | 2.65 | 3.05 | -0.80 | -3.51 | -3.95 | -1.66 | -3.59 | -3.48 | -1.40 | 2.46 | -0.84 | -2.58 | -4.63 | -3.73 | -2.14 |
Cell Periphery | 4.28 | 5.86 | -1.30 | -3.73 | -4.34 | -0.20 | -3.35 | -5.70 | -5.73 | -4.31 | 1.41 | 2.47 | -4.58 | -4.99 | -5.29 |
Cytoplasm | 1.75 | 1.43 | 0.55 | -2.77 | -1.85 | -1.56 | -2.30 | -1.06 | 1.22 | 1.98 | -1.09 | -0.87 | -1.31 | 0.34 | -0.55 |
Cytoplasmic Foci | 5.23 | -0.63 | 7.08 | 3.97 | 11.65 | -3.24 | -4.94 | 0.80 | 4.14 | 5.06 | -0.45 | -10.40 | 4.77 | 5.75 | 13.21 |
Eisosomes | -0.32 | 1.43 | 0.41 | 1.53 | -2.10 | 1.19 | 0.06 | 3.30 | 3.31 | 3.29 | -0.70 | 1.43 | 1.30 | 2.81 | 0.03 |
Endoplasmic Reticulum | 5.40 | 5.32 | 4.62 | -2.64 | -2.35 | -0.12 | -2.91 | -2.46 | -2.27 | 1.66 | 4.60 | 3.06 | 2.15 | -3.30 | -0.97 |
Endosome | 9.46 | 7.91 | 9.53 | 0.06 | 4.18 | -4.42 | -5.25 | -6.36 | -2.45 | 3.74 | 3.38 | -3.67 | 2.07 | -1.63 | 5.79 |
Golgi | 13.33 | 10.21 | 12.70 | -2.17 | 6.96 | -4.68 | -3.99 | -7.57 | -3.50 | 0.80 | 6.99 | -3.23 | 3.90 | -4.06 | 7.38 |
Lipid Particles | -4.05 | -6.83 | 1.11 | 4.38 | 7.22 | -0.38 | 0.80 | 6.77 | 4.98 | 4.29 | -2.14 | -3.97 | 6.34 | 6.53 | 8.26 |
Mitochondria | -17.66 | -10.11 | -16.93 | 1.75 | -12.36 | 5.45 | 7.77 | 7.59 | 1.91 | -5.93 | -5.11 | 12.72 | -3.07 | 2.55 | -16.11 |
Mitotic Spindle | 3.72 | -0.57 | 1.97 | -4.80 | 3.27 | -2.85 | -4.30 | -4.55 | -2.88 | 3.73 | 1.99 | -6.08 | -2.88 | -5.42 | 5.04 |
None | -0.10 | -9.29 | 0.63 | 0.70 | 10.48 | -0.35 | -0.38 | 6.32 | 1.71 | 5.15 | -0.36 | -9.60 | 4.52 | 1.84 | 12.48 |
Nuclear Periphery | 2.32 | -3.51 | 2.14 | -3.60 | 5.32 | 1.91 | -3.30 | 1.02 | -0.97 | 3.37 | 2.72 | -5.36 | 2.13 | -3.17 | 5.66 |
Nuclear Periphery Foci | 2.33 | -1.58 | 2.56 | 1.27 | 5.32 | -1.15 | -4.27 | 0.06 | 1.17 | 4.28 | 1.54 | -5.06 | 2.35 | 1.65 | 5.76 |
Nucleolus | -2.11 | -2.39 | -0.71 | 2.02 | 2.19 | -1.76 | -1.24 | -0.66 | 1.45 | 1.22 | -2.37 | -2.69 | -1.06 | 2.23 | 2.57 |
Nucleus | -1.56 | -2.77 | -0.30 | 1.47 | 1.81 | -2.00 | -1.52 | -0.99 | 1.95 | 1.45 | -2.57 | -3.87 | -1.38 | 2.42 | 3.05 |
Peroxisomes | -5.94 | -2.01 | -0.96 | 5.54 | 1.13 | -1.20 | -3.90 | 0.37 | 1.49 | 3.97 | -5.31 | -3.46 | -0.01 | 5.39 | 3.52 |
Vacuole | 8.76 | 3.86 | 6.68 | -6.04 | 6.19 | -4.62 | -4.47 | -5.58 | -2.50 | 3.00 | 5.85 | -8.33 | 0.40 | -6.18 | 8.91 |
Vacuole Periphery | 11.23 | 2.26 | 8.58 | -9.44 | 11.11 | -2.03 | -4.55 | -7.21 | -5.32 | 2.49 | 9.06 | -11.87 | 1.59 | -10.72 | 13.31 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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